Stem cell ultrasonic disruption device and method

Abstract

The invention relates to the field of ultrasonic cell disruption, and particularly discloses an ultrasonic stem cell disruption device and an ultrasonic stem cell disruption method. Comprising a control cabinet body, an ultrasonic generator arranged at the upper end of the control cabinet body, a cell sap container body arranged on the inner side of the control cabinet body and an ultrasonic probe arranged at the bottom of the ultrasonic generator and extending into an inner cavity of the cell sap container body. A top cover is arranged on the top of the cell sap container body in a pressing mode, an anti-deposition mechanism extending to an inner cavity of the cell sap container body is arranged at the bottom of the top cover, and a lifting mechanism used for driving the cell sap container body to ascend and descend is arranged on the inner side of the control cabinet body. According to the stem cell ultrasonic disruption device, the anti-deposition mechanism is matched with the lifting of the cell sap container body, so that bottom deposited cell sap can be automatically conveyed to the direction of the ultrasonic probe, and meanwhile, due to the lifting of the cell sap container body, the position of the ultrasonic probe in the cell sap container body is continuously changed; and the cell disruption effect and efficiency can be improved.

Description

Technical Field

[0001] The present invention relates to the field of ultrasonic cell disruption, and in particular to a device and method for ultrasonic cell disruption. Background Art

[0002] The ultrasonic disruption device for stem cells is a device that uses ultrasonic energy to disrupt cells and is widely used in biomedical research, cell engineering and other fields. Existing ultrasonic disruption devices are usually composed of core components such as ultrasonic generators, ultrasonic probes, and control systems. The ultrasonic probe produces a cavitation effect through high-frequency vibrations, which can effectively disrupt cells in the cell fluid in a local area.

[0003] However, in existing devices, the size of the cell fluid container is usually much larger than the ultrasonic probe, and the effective range of the ultrasonic probe is limited. It can only produce a significant fragmentation effect on the cell fluid near the probe. For areas far away from the probe, especially cells at the bottom or edge of the container, the fragmentation effect of ultrasound is significantly reduced. This limitation causes some cells to be unable to be effectively processed, especially when the cells are deposited at the bottom of the container or in a peripheral dead corner, the fragmentation effect is poor. Some existing devices are usually equipped with a local lifting function, and the ultrasonic probe can be lifted and lowered along the axis of the container. However, this lifting function is usually linear or uniaxial movement, and the probe can only move along a fixed path, and cannot flexibly cover the entire area of ​​the container. For cells deposited at the bottom or distributed around the periphery of the container, the fragmentation effect is poor because the probe is difficult to reach.

[0004] Therefore, it is necessary to propose a stem cell ultrasonic fragmentation device and method to solve the above problems. Summary of the invention

[0005] The main purpose of the present invention is to provide a stem cell ultrasonic fragmentation device and method, which can effectively solve the problems in the background technology.

[0006] To achieve the above object, the technical solution adopted by the present invention is: A stem cell ultrasonic pulverization device comprises a control cabinet, an ultrasonic generator arranged at the upper end of the control cabinet, a cell fluid container body arranged inside the control cabinet, and an ultrasonic probe arranged at the bottom of the ultrasonic generator and extending to the inner cavity of the cell fluid container body; The top of the cell fluid container body is tightly provided with a top cover, and the bottom of the top cover is provided with an anti-deposition mechanism extending to the inner cavity of the cell fluid container body, the anti-deposition mechanism includes a cylinder fixed at the bottom of the top cover, a piston is movably provided on the inner side of the cylinder, a piston rod extending to the top of the top cover is provided on the top of the piston, a third one-way valve is provided on the inner side of the lower end of the cylinder, the bottom of the cylinder is connected with a suction nozzle, the end of the cylinder close to the ultrasonic probe is connected with a first connecting pipe, the first connecting pipe is provided with a first one-way valve, the end of the first connecting pipe away from the cylinder is connected with a nozzle, the side of the cylinder is connected with a second connecting pipe, the second connecting pipe is provided with a second one-way valve, the lower end of the second connecting pipe is located below the third one-way valve, the inner upper end of the control cabinet is provided with a top plate located on the top of the top cover, the upper end of the piston rod is fixed to the bottom wall of the top plate, and the inner side of the control cabinet is provided with a lifting mechanism for driving the cell fluid container body to rise and fall.

[0007] Preferably, the lifting mechanism includes a floating seat arranged at the bottom inner side of the control cabinet, the bottom of the floating seat is provided with a second driving source for driving the floating seat to lift and lower, the periphery of the floating seat is provided with a connecting frame, and the upper end of the connecting frame is fixedly connected to the outer side wall of the top cover.

[0008] Preferably, the second one-way valve is configured so that the cell fluid can only enter the cylinder from the second connecting pipe; The first one-way valve is configured so that the cell fluid can only enter the first connecting tube from the cylinder; The third one-way valve is configured so that the cell fluid can only enter the cylinder from the suction nozzle.

[0009] Preferably, a cooling circulation pipe located outside the cell liquid container body is provided on the upper end side of the connecting frame, a refrigeration compressor is provided on one side of the control cabinet, and both ends of the cooling circulation pipe are connected to the refrigeration compressor through a hose.

[0010] Preferably, a feeding mechanism is provided on the side of the cylinder, and the feeding mechanism includes a hollow plate arranged on the side of the cylinder away from the ultrasonic probe, the inner side of the hollow plate is vertically movably connected with a lifting frame, the top of the lifting frame is provided with a connecting rod extending to above the top cover and connected to the bottom wall of the top plate, the side of the hollow plate away from the cylinder is rotatably provided with a transversely arranged rotating shaft, the end of the rotating shaft away from the cylinder is provided with a feeding paddle, the end of the rotating shaft close to the cylinder extends to the inner side of the hollow plate and is provided with a pair of gears, a one-way bearing is fixed at the corresponding position of the outer wall of the rotating shaft and the gear, the two one-way bearings on the rotating shaft rotate in opposite directions, the gear is fixed on the one-way bearing, and a pair of oppositely arranged toothed plates are provided on the side of the lifting frame close to the rotating shaft, and the gear teeth on the two toothed plates correspond to and mesh with the two gears respectively.

[0011] Preferably, the lower end of the cylinder is provided with an adjustment component for adjusting the angle of the suction nozzle, the adjustment component includes a connecting seat arranged at the bottom of the cylinder, the connecting seat is a hollow structure and is connected to the cylinder, the third one-way valve is located at the upper end of the connecting seat, the lower end of the second connecting pipe is connected to the connecting seat, a universal ball is movably provided on the inner side of the lower end of the connecting seat, the suction nozzle is fixed to the bottom of the universal ball, the universal ball is provided with a through hole connected with the suction nozzle and the connecting seat, an arc-shaped force-bearing frame is provided on one side of the suction nozzle, and a driving rod extending to the bottom of the hollow plate and corresponding to the force-bearing frame is provided at the bottom of the lifting frame.

[0012] Preferably, a reset assembly is provided on the side of the universal ball, and the reset assembly includes a fixed sleeve arranged on the side wall of the connecting seat, and the side wall of the universal ball is provided with a connecting pin movably connected to the inner side of the fixed sleeve, and a torsion spring is fixed between one end of the connecting pin close to the fixed sleeve and the inner wall of the fixed sleeve.

[0013] Preferably, a pushing and clamping mechanism is provided at the bottom of the cell fluid container body, and the pushing and clamping mechanism includes a support plate which is vertically movably arranged between the floating seat and the cell fluid container body and is used to support the bottom of the cell fluid container body, and a first driving source for driving the support plate to rise and fall is provided on the top of the floating seat.

[0014] Preferably, a limiting mechanism for limiting the cell fluid container body is provided on the support plate, and the limiting mechanism includes a groove arranged on the top of the support plate, a supporting ring corresponding to the cell fluid container body is vertically movably provided on the inner side of the groove, and a groove connected to the groove is provided on the periphery of the support plate, and a positioning arm in an "L" shape and used for clamping the lower end side wall of the cell fluid container body is rotatably provided on the inner side of the groove, and the lower end of the positioning arm is located below the supporting ring, and a tension spring is fixed between the bottom of one end of the positioning arm and the bottom wall of the groove.

[0015] Another embodiment is also provided, specifically a method for ultrasonically disrupting stem cells, comprising the following steps: S1: The cell fluid is loaded into the cell fluid container body, and the cell fluid container body is placed on the top of the support plate; S2: Pressing the cell solution container body by pushing and pressing the mechanism in cooperation with the top cover; S3: The lifting mechanism controls the overall lifting of the cell solution container body, the top cover, and the support plate. According to the lifting, the anti-deposition mechanism automatically transports the cell solution deposited at the bottom of the cell solution container body to the vicinity of the ultrasonic probe.

[0016] Compared with the prior art, the present invention provides a stem cell ultrasonic fragmentation device and method, which has the following beneficial effects: The stem cell ultrasonic fragmentation device can automatically transport the cell fluid deposited at the bottom toward the ultrasonic probe by cooperating with the lifting of the cell fluid container body through the anti-deposition mechanism. At the same time, due to the lifting of the cell fluid container body, the ultrasonic probe constantly changes its position in the cell fluid container body, which can improve the cell fragmentation effect and efficiency.

[0017] The stem cell ultrasonic pulverization device can transport the cell fluid in the middle of the cell fluid container body to the periphery according to the lifting and lowering of the cell fluid container body through the provided feeding mechanism, so as to facilitate the uniform cooling of the cooling circulation pipe, solve the shortcoming of the prior art that it can only cool the periphery locally, and avoid the high-temperature inactivation of cells. At the same time, the feeding mechanism cooperates with the adjustment component to continuously change the angle of the suction nozzle during feeding, thereby increasing the suction range of the suction nozzle. The structure is compact, the linkage is high, and it is easy to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the structure of the present invention; Figure 2 It is a structural schematic diagram of the present invention in a state where the control cabinet is removed; Figure 3 It is a schematic diagram of the structure of the present invention when the floating seat and the guide cylinder are separated; Figure 4 The present invention Figure 3 A structural diagram from another perspective based on the above; Figure 5 It is a schematic diagram of the structure of the present invention in which the top cover and the cell fluid container body are separated; Figure 6 It is a schematic diagram of the structure of the top cover of the present invention; Figure 7 It is a schematic diagram of the overall structure of the cylinder and the hollow plate of the present invention; Figure 8 It is a schematic diagram of the structure inside the cylinder of the present invention; Fig. 9 It is a schematic diagram of the structure of the suction nozzle and the connecting base of the present invention in a separated state; Fig.10 It is a schematic diagram of the structure of the lifting frame, hollow plate and rotating shaft of the present invention in a disassembled state; Fig.11 It is a schematic diagram of the structure of the tooth plate and the gear of the present invention after being disassembled from above; Fig.12 It is a schematic diagram of the cross-sectional structure of the support plate of the present invention.

[0019] In the figure: 1, control cabinet; 2, refrigeration compressor; 3, ultrasonic generator; 4, cell liquid container body; 5, cooling circulation pipe; 6, floating seat; 7, connecting frame; 8, first driving source; 9, top cover; 10, top plate; 11, fixing frame; 12, supporting plate; 13, tension spring; 14, guide cylinder; 15, guide groove; 16, guide block; 17, second driving source; 18, piston rod; 19, connecting rod; 20, cylinder; 21, hollow plate; 22, suction nozzle; 23, feeding paddle; 2 4. Nozzle; 25. First connecting pipe; 26. First one-way valve; 27. Second connecting pipe; 28. Second one-way valve; 29. ​​Piston; 30. Force frame; 31. Universal ball; 32. Connecting seat; 33. Third one-way valve; 34. Through hole; 35. Fixed sleeve; 36. Torsion spring; 37. Connecting pin; 38. Lifting frame; 39. Rotating shaft; 40. Gear; 41. Tooth plate; 42. Groove; 43. Support ring; 44. Slot; 45. Positioning arm; 46. Ultrasonic probe; 47. Driving rod. DETAILED DESCRIPTION

[0020] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0021] like Figure 1-Figure 6 As shown, a stem cell ultrasonic pulverization device comprises a control cabinet 1, an ultrasonic generator 3 arranged at the upper end of the control cabinet 1, a cell fluid container body 4 arranged inside the control cabinet 1, and an ultrasonic probe 46 arranged at the bottom of the ultrasonic generator 3 and extending to the inner cavity of the cell fluid container body 4, a top cover 9 is tightly arranged on the top of the cell fluid container body 4, a floating seat 6 is arranged at the bottom of the inner side of the control cabinet 1, and a second driving source 17 for driving the floating seat 6 to rise and fall is arranged at the bottom of the floating seat 6, and the second driving source 17 is preferably a linear motor or a cylinder, and the periphery of the floating seat 6 is arranged There is a connecting frame 7, the upper end of the connecting frame 7 is fixedly connected to the outer side wall of the top cover 9, and a pushing and pressing mechanism is arranged at the bottom of the cell liquid container body 4, and the pushing and pressing mechanism includes a supporting plate 12 which is vertically movably arranged between the floating seat 6 and the cell liquid container body 4 and is used to support the bottom of the cell liquid container body 4, and a first driving source 8 for driving the supporting plate 12 to rise and fall is arranged on the top of the floating seat 6, and the first driving source 8 is preferably a cylinder, and a hole corresponding to the ultrasonic probe 46 is arranged on the top cover 9, and the ultrasonic probe 46 extends to the inner cavity of the cell liquid container body 4 through the hole on the top cover 9; like Fig.12As shown, in order to ensure the radial stability of the cell liquid container body 4, a limiting mechanism for limiting the cell liquid container body 4 is provided on the support plate 12, and the limiting mechanism includes a groove 42 provided on the top of the support plate 12, and a supporting ring 43 corresponding to the cell liquid container body 4 is vertically movably provided inside the groove 42, and a groove 44 connected to the groove 42 is provided on the periphery of the support plate 12, and a positioning arm 45 in an "L" shape and used for clamping the lower end side wall of the cell liquid container body 4 is rotatably provided inside the groove 44, and the lower end of the positioning arm 45 is located below the supporting ring 43, and a tension spring 13 is fixed between the bottom of one end of the positioning arm 45 and the bottom wall of the groove 44, and the tension spring 13 is used for resetting the positioning arm 45 and the supporting ring 43; like Figure 6-Figure 8 As shown, the bottom of the top cover 9 is provided with an anti-deposition mechanism extending to the inner cavity of the cell fluid container body 4, the anti-deposition mechanism includes a cylinder 20 uniformly surrounded and fixed on the outer periphery of the bottom of the top cover 9, the cylinder 20 is vertically arranged, and a piston 29 is vertically and movably arranged inside the cylinder 20, and a piston rod 18 extending to the top of the top cover 9 is arranged on the top of the piston 29, and the piston rod 18 and the top cover 9 are movably guided, and a third one-way valve 33 is arranged on the inner side of the lower end of the cylinder 20, and the third one-way valve 33 is configured so that the cell fluid can only enter the cylinder 20 from the suction nozzle 22, and the bottom of the cylinder 20 is connected to the suction nozzle 22 in a trumpet shape, and the end of the cylinder 20 on one side close to the ultrasonic probe 46 is connected to the first connecting pipe 25, and the first connecting pipe 25 is provided with a first single The first one-way valve 26 is configured so that the cell fluid can only enter the first connecting tube 25 from the cylinder 20. The end of the first connecting tube 25 away from the cylinder 20 is connected with a nozzle 24 corresponding to the ultrasonic probe 46. The side of the cylinder 20 is connected with a second connecting tube 27. The upper end of the second connecting tube 27 is connected with the upper end of the cylinder 20, and the lower end is connected with the lower end of the cylinder 20. The second connecting tube 27 is provided with a second one-way valve 28. The second one-way valve 28 is configured so that the cell fluid can only enter the cylinder 20 from the second connecting tube 27. The lower end of the second connecting tube 27 is located below the third one-way valve 33. The upper inner end of the control cabinet 1 is provided with a top plate 10 located on the top of the top cover 9, and the upper end of the piston rod 18 is fixed to the bottom wall of the top plate 10.

[0022] like Figure 1-Figure 2 , Figure 6-Figure 7 , Figure 10-11As shown, in order to cool down the heat generated during the crushing process, a cooling circulation pipe 5 located outside the cell liquid container body 4 is arranged on the upper end side of the connecting frame 7, and a refrigeration compressor 2 is arranged on one side of the control cabinet 1. Both ends of the cooling circulation pipe 5 are connected to the refrigeration compressor 2 through a hose. In order to make the cooling uniform, a feeding mechanism is arranged on the side of the cylinder 20, and the feeding mechanism includes a hollow plate 21 arranged on the side of the cylinder 20 away from the ultrasonic probe 46. The inner side of the hollow plate 21 is vertically and movably connected with a lifting frame 38. The top of the lifting frame 38 is provided with a connecting rod 19 extending to the top of the top cover 9 and connected to the bottom wall of the top plate 10, and the connecting rod 19 and the top cover 9 are movable guides. A transversely arranged rotating shaft 39 is rotatably arranged on the side of the cylinder 21 away from the cylinder 20, a feeding paddle 23 is arranged on the end of the rotating shaft 39 away from the cylinder 20, and an end of the rotating shaft 39 close to the cylinder 20 extends to the inner side of the hollow plate 21 and is provided with a pair of gears 40, a one-way bearing is fixed at the corresponding position of the outer wall of the rotating shaft 39 and the gear 40, the two one-way bearings on the rotating shaft 39 rotate in opposite directions, the gear 40 is fixed on the one-way bearing, and a pair of oppositely arranged toothed plates 41 are arranged on the side of the lifting frame 38 close to the rotating shaft 39, the gear teeth on the two toothed plates 41 respectively correspond to and mesh with the two gears 40, so that the rotating shaft 39 can always rotate in one direction when the lifting frame 38 is lifted and lowered relative to the hollow plate 21.

[0023] like Figure 8-Figure 10 As shown in the figure, as an embodiment, different from the above, in order to increase the suction range of the suction nozzle 22, an adjustment component for adjusting the angle of the suction nozzle 22 is provided at the lower end of the cylinder 20, and the adjustment component includes a connecting seat 32 arranged at the bottom of the cylinder 20, the connecting seat 32 is a hollow structure and is connected to the cylinder 20, the third one-way valve 33 is located at the upper end of the connecting seat 32, the lower end of the second connecting pipe 27 is connected to the connecting seat 32, and a universal ball 31 is movably provided on the inner side of the lower end of the connecting seat 32, the suction nozzle 22 is fixed to the bottom of the universal ball 31, and the universal ball 31 is provided with a suction A through hole 34 connecting the nozzle 22 and the connecting seat 32, an arc-shaped force frame 30 is arranged on one side of the suction nozzle 22, and a driving rod 47 extending to the bottom of the hollow plate 21 and corresponding to the force frame 30 is arranged at the bottom of the lifting frame 38. In order to facilitate the resetting of the suction nozzle 22, a reset component is arranged on the side of the universal ball 31, and the reset component includes a fixed sleeve 35 arranged on the side wall of the connecting seat 32, and a connecting pin 37 movably connected to the inner side of the fixing sleeve 35 is arranged on the side wall of the universal ball 31, and a torsion spring 36 is fixed between one end of the connecting pin 37 close to the fixing sleeve 35 and the inner wall of the fixing sleeve 35.

[0024] In addition, if Figure 3-Figure 4As shown, in order to further increase the flow complexity of the cell fluid in the cell fluid container body 4, a guide cylinder 14 located on the outside of the floating seat 6 is provided at the lower end of the inner side of the control cabinet 1, and the floating seat 6 is movably guided by a guide block 16. A spiral guide groove 15 is provided on the side of the floating seat 6, and a guide block 16 movably guided by the guide groove 15 is provided on the inner wall of the guide cylinder 14. Therefore, when the floating seat 6 is raised and lowered, it will also drive the cell fluid container body 4 to reciprocate, and in order to make the top plate 10 adapt to the rotation of the cell fluid container body 4, a fixing frame 11 is fixed to the upper end of the inner side of the control cabinet 1, and the top plate 10 is rotatably set on the fixing frame 11 so that it can follow the rotation. It should be noted that the cooling circulation pipe 5 is connected to the refrigeration compressor 2 through a hose and will not be affected by the rotation. The present application also includes an embodiment, specifically a stem cell ultrasonic fragmentation method, using the above-mentioned stem cell ultrasonic fragmentation device, comprising the following steps: Step 1: The cell fluid is loaded into the cell fluid container body 4, and the cell fluid container body 4 is placed on the top of the support plate 12; Step 2: Press the cell liquid container body 4 by pushing and pressing the mechanism in cooperation with the top cover 9; Step 3: The lifting mechanism controls the overall lifting of the cell fluid container body 4 , the top cover 9 , and the support plate 12 . According to the lifting, the anti-deposition mechanism automatically transports the cell fluid deposited at the bottom of the cell fluid container body 4 to the vicinity of the ultrasonic probe 46 .

[0025] Working principle: when in use, the cell fluid to be crushed is loaded into the cell fluid container body 4, the cell fluid container body 4 is placed on the top of the support plate 12, the first driving source 8 is controlled to drive the support plate 12 to rise, and the support plate 12 drives the cell fluid container body 4 to rise until the top cover 9 presses the top of the cell fluid container body 4. At this time, the ultrasonic probe 46 and the cylinder 20 are both located in the inner cavity of the cell fluid container body 4, and because the cell fluid container body 4 is under pressure, the support ring 43 will be compressed downward, and the bottom edge of the support ring 43 will press the positioning arm 45 to rotate, and the other end of the positioning arm 45 will flip toward the direction of the cell fluid container body 4, thereby clamping the lower end side wall of the cell fluid container body 4, increasing the radial stability of the cell fluid container body 4, and avoiding displacement caused by subsequent lifting. After the cutting is ready, the second driving source 17 is controlled to drive the floating seat 6, the cell liquid container body 4, the support plate 12, and the top cover 9 to reciprocate and rise and fall, and the top plate 10 is located above the top cover 9 and the height remains unchanged, and the cylinder 20 is fixed to the bottom wall of the top cover 9, and the upper ends of the piston rod 18 and the connecting rod 19 are fixed to the bottom wall of the top plate 10. Therefore, when the cell liquid container body 4 and the top cover 9 are lifted and lowered, the cylinder 20 will be relatively displaced with the piston rod 18 and the piston 29, and the hollow plate 21 will be relatively displaced with the connecting rod 19 and the lifting frame 38. Due to the action of the first one-way valve 26, the second one-way valve 28, and the third one-way valve 33, when the piston 29 moves downward relative to the cylinder 20, negative pressure is generated above the piston 29, and the second connecting pipe 27 sucks the cell liquid into the cylinder 2 through the suction nozzle 22. 0, and the cell fluid at the lower end of the inner cavity of the cylinder 20 is pushed out by the piston 29 into the first connecting tube 25 at the lower end, and then sprayed out from the first connecting tube 25 to the vicinity of the ultrasonic probe 46. When the piston 29 moves upward relative to the cylinder 20, negative pressure is generated below the piston 29, and the suction nozzle 22 adsorbs the cell fluid to the bottom of the piston 29 inside the cylinder 20. The cell fluid at the upper end of the inner cavity of the cylinder 20 is pushed out by the piston 29 from the first connecting tube 25 at the upper end, and finally sprayed out from the nozzle 24 to the vicinity of the ultrasonic probe 46. Regardless of rising or falling, the deposited cells can be continuously transported to the vicinity of the ultrasonic probe 46. During this period, when the lifting frame 38 and the hollow plate 21 are relatively displaced, the tooth plate 41 and the gear 40 are continuously driven to cooperate. The rotating shaft 39 rotates, and since the gear 40 is fixed to the outer wall of the rotating shaft 39 through a one-way bearing, no matter whether the lifting frame 38 moves upward or downward relative to the hollow plate 21, the two tooth plates 41 can alternately drive the rotating shaft 39 to rotate through the corresponding gears 40, and then the feeding blades 23 always rotate in one direction, thereby transporting the material in the middle of the cell liquid container body 4 to the periphery. The material transported to the periphery contacts the side wall of the cell liquid container body 4, and then can be cooled by the cooling circulation pipe 5, which increases the contact opportunity between the cell liquid and the outer wall of the cell liquid container body 4, facilitates uniform cooling, avoids overheating during ultrasonic fragmentation and causes cell inactivation, and part of the cell liquid that collides with the outer wall of the cell liquid container body 4 will flow to the bottom of the inner cavity of the cell liquid container body 4.Then the suction nozzle 22 continues to suck the ultrasonic probe 46, and this cycle not only improves the crushing effect and efficiency, but also increases the cooling effect; In addition, when the lifting frame 38 is lifted or lowered relative to the hollow plate 21, the driving rod 47 will continuously push the force frame 30. Under the action of the universal ball 31, the suction nozzle 22 will continuously change its inclination angle, thereby increasing the suction range. Moreover, when the universal ball 31 rotates, it will torsion spring 36 through connecting pin 37. When the driving rod 47 is separated from the force frame 30, the torsion spring 36 will reset, and then the universal ball 31 will drive the suction nozzle 22 to reset. In this way, the lifting and lowering of the lifting frame 38 can be guided by the driving rod 47 and the connecting rod 19, thereby increasing stability. In order to further increase the flow complexity of the cell fluid, when the second driving source 17 drives the floating seat 6 to rise and fall, due to the action of the guide block 16 and the guide groove 15, the floating seat 6, the top cover 9, the support plate 12, and the cell fluid container body 4 will also reciprocate as a whole, further increasing the flow complexity of the cell fluid, which is beneficial to crushing and cooling.

[0026] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A stem cell ultrasonic disruption device, characterized in that: It includes a control cabinet, an ultrasonic generator arranged at the upper end of the control cabinet, a cell fluid container body arranged inside the control cabinet, and an ultrasonic probe arranged at the bottom of the ultrasonic generator and extending to the inner cavity of the cell fluid container body; The top of the cell fluid container body is tightly provided with a top cover, and the bottom of the top cover is provided with an anti-deposition mechanism extending to the inner cavity of the cell fluid container body, the anti-deposition mechanism includes a cylinder fixed at the bottom of the top cover, a piston is movably provided on the inner side of the cylinder, a piston rod extending to the top of the top cover is provided on the top of the piston, a third one-way valve is provided on the inner side of the lower end of the cylinder, the bottom of the cylinder is connected with a suction nozzle, the end of the cylinder close to the ultrasonic probe is connected with a first connecting pipe, the first connecting pipe is provided with a first one-way valve, the end of the first connecting pipe away from the cylinder is connected with a nozzle, the side of the cylinder is connected with a second connecting pipe, the second connecting pipe is provided with a second one-way valve, the lower end of the second connecting pipe is located below the third one-way valve, the inner upper end of the control cabinet is provided with a top plate located on the top of the top cover, the upper end of the piston rod is fixed to the bottom wall of the top plate, and the inner side of the control cabinet is provided with a lifting mechanism for driving the cell fluid container body to rise and fall.

2. A stem cell ultrasonic disruption device according to claim 1, characterized in that: The lifting mechanism includes a floating seat arranged at the bottom inner side of the control cabinet, a second driving source for driving the floating seat to lift is arranged at the bottom of the floating seat, a connecting frame is arranged at the periphery of the floating seat, and the upper end of the connecting frame is fixedly connected to the outer side wall of the top cover.

3. The stem cell ultrasonic disruption device according to claim 1, characterized in that: The second one-way valve is configured so that the cell fluid can only enter the cylinder from the second connecting pipe; The first one-way valve is configured so that the cell fluid can only enter the first connecting tube from the cylinder; The third one-way valve is configured so that the cell fluid can only enter the cylinder from the suction nozzle.

4. The stem cell ultrasonic disruption device according to claim 2, characterized in that: A cooling circulation pipe located outside the cell liquid container body is arranged on the upper end side of the connecting frame, a refrigeration compressor is arranged on one side of the control cabinet, and both ends of the cooling circulation pipe are connected to the refrigeration compressor through a hose.

5. The stem cell ultrasonic disruption device according to claim 4, characterized in that: A feeding mechanism is provided on the side of the cylinder, and the feeding mechanism includes a hollow plate arranged on the side of the cylinder away from the ultrasonic probe, a lifting frame is vertically and movably connected to the inner side of the hollow plate, a connecting rod extending to the top of the top cover and connected to the bottom wall of the top plate is provided on the top of the lifting frame, a horizontally arranged rotating shaft is rotatably provided on the side of the hollow plate away from the cylinder, a feeding paddle is provided on the end of the rotating shaft away from the cylinder, an end of the rotating shaft close to the cylinder extends to the inner side of the hollow plate and is provided with a pair of gears, a one-way bearing is fixed at the corresponding position of the outer wall of the rotating shaft and the gear, the two one-way bearings on the rotating shaft rotate in opposite directions, the gear is fixed on the one-way bearing, and a pair of oppositely arranged toothed plates are provided on the side of the lifting frame close to the rotating shaft, and the gear teeth on the two toothed plates correspond to and mesh with the two gears respectively.

6. The stem cell ultrasonic disruption device according to claim 5, characterized in that: The lower end of the cylinder is provided with an adjustment component for adjusting the angle of the suction nozzle, the adjustment component includes a connecting seat arranged at the bottom of the cylinder, the connecting seat is a hollow structure and is connected to the cylinder, the third one-way valve is located at the upper end of the connecting seat, the lower end of the second connecting pipe is connected to the connecting seat, a universal ball is movably provided on the inner side of the lower end of the connecting seat, the suction nozzle is fixed to the bottom of the universal ball, the universal ball is provided with a through hole connected with the suction nozzle and the connecting seat, an arc-shaped force-bearing frame is provided on one side of the suction nozzle, and a driving rod extending to the bottom of the hollow plate and corresponding to the force-bearing frame is provided at the bottom of the lifting frame.

7. The stem cell ultrasonic disruption device according to claim 6, characterized in that: A reset assembly is arranged on the side of the universal ball, and the reset assembly includes a fixed sleeve arranged on the side wall of the connecting seat. The side wall of the universal ball is provided with a connecting pin movably connected to the inner side of the fixed sleeve. A torsion spring is fixed between one end of the connecting pin close to the fixed sleeve and the inner wall of the fixed sleeve.

8. The stem cell ultrasonic disruption device according to claim 2, characterized in that: A pushing and clamping mechanism is arranged at the bottom of the cell fluid container body, and the pushing and clamping mechanism includes a support plate which is vertically movably arranged between the floating seat and the cell fluid container body and is used to support the bottom of the cell fluid container body. A first driving source for driving the support plate to rise and fall is arranged on the top of the floating seat.

9. The stem cell ultrasonic disruption device according to claim 8, characterized in that: The support plate is provided with a limiting mechanism for limiting the cell fluid container body, and the limiting mechanism includes a groove arranged on the top of the support plate, and a supporting ring corresponding to the cell fluid container body is vertically movably arranged on the inner side of the groove, and a groove connected to the groove is arranged on the periphery of the support plate, and a positioning arm in an "L" shape and used for clamping the lower end side wall of the cell fluid container body is rotatably arranged on the inner side of the groove, and the lower end of the positioning arm is located below the supporting ring, and a tension spring is fixed between the bottom of one end of the positioning arm and the bottom wall of the groove.

10. A method for ultrasonically disrupting stem cells, implemented using the ultrasonic disrupting device for stem cells according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: The cell fluid is loaded into the cell fluid container body, and the cell fluid container body is placed on the top of the support plate; S2: Pressing the cell solution container body by pushing and pressing the mechanism in cooperation with the top cover; S3: The lifting mechanism controls the overall lifting of the cell solution container body, the top cover, and the support plate. According to the lifting, the anti-deposition mechanism automatically transports the cell solution deposited at the bottom of the cell solution container body to the vicinity of the ultrasonic probe.

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