Cell detection device

Abstract

The invention discloses a cell detection device, and belongs to the technical field of cell culture detection, the cell detection device comprises an incubator, a driving mechanism is fixedly connected in the incubator, a movable supporting plate is arranged on the driving mechanism, a plurality of culture racks are fixedly connected in the incubator, and a plurality of culture plates are arranged on the culture racks. Through the use of the driving mechanism and the culture mechanism, when the culture plate needs to be taken, the driving mechanism drives the movable supporting plate to move to the position below the culture plate, then the movable supporting plate is jacked upwards, so that the culture plate is separated from the fixing frame, and then under the driving of the driving mechanism, the movable supporting plate drives the culture plate to move to the position close to the turning plate; the culture plate can be taken out by opening the turning plate through the handle, the incubator does not need to be opened for a long time, the internal environment of the incubator is more stable, meanwhile, manual searching is not needed, the possibility that cells are polluted is greatly reduced, and the detection effect is more accurate.

Description

Technical Field

[0001] The invention belongs to the technical field of cell culture detection, and in particular relates to a cell detection device. Background Art

[0002] Cells need to be cultured before testing, mainly to ensure that they are in a state suitable for the experiment. Most cells need to be in a suitable environment to maintain vitality and physiological functions. The culture process helps cells maintain their activity and ensures the reliability of experimental results by providing nutrients, temperature, humidity, pH and other conditions suitable for cell growth. Many experiments require a certain number of cells to obtain sufficient experimental data. Through culture, researchers can expand the number of cells to meet subsequent experimental needs. If the cells come from frozen storage or passaging, they may experience a period of dormancy or damage before certain experiments. Through culture, cells can restore their normal growth state to ensure that they are in the best condition for experiments. In some experiments, researchers need specific stimulation or treatment (such as drugs, hormones, environmental changes, etc.) to change the state of cells. The culture stage provides time and space conditions for this specific induction, so culture before cell testing is an essential step.

[0003] However, when a large number of cells need to be tested, the cells need to be frequently removed and replaced from the culture device, which requires the hatch of the incubator to be opened for a long time. At the same time, the manual search process is likely to contaminate the cell culture process. The long-term opening of the hatch causes the environment inside the incubator to be unstable, which reduces cell activity and affects the detection effect. Summary of the invention

[0004] The technical problem to be solved by the present invention is to overcome the disadvantages of the above-mentioned prior art and provide a cell detection device.

[0005] The technical solution adopted to solve the above technical problems is: a cell detection device, comprising an incubator, a driving mechanism is fixedly connected in the incubator, a movable support plate is arranged on the driving mechanism, a plurality of culture racks are fixedly connected in the incubator, a plurality of culture plates are arranged on the culture racks, a plurality of flaps are rotatably connected to one side of the incubator, and a handle is fixedly connected to each of the flaps;

[0006] The culture rack includes a fixed frame fixedly connected in the incubator, a plurality of fixed sleeves are fixedly connected to the lower surface of the fixed frame, a wedge is slidably connected in the fixed sleeve, and the culture plate includes a base arranged below the fixed sleeve, a fixed column is fixedly connected to the base, a thin rod is fixedly connected to the upper end of the fixed column, a frustum is fixedly connected to the upper end of the thin rod, and a wedge-shaped ring is slidably connected through the outside of the thin rod.

[0007] Through the above technical scheme, through the use of the driving mechanism and the culture mechanism, when the culture plate needs to be taken, the driving mechanism drives the movable support plate to move to the bottom of the culture plate, and then the movable support plate is pushed upward to separate the culture plate from the fixed frame. Then, driven by the driving mechanism, the movable support plate drives the culture plate to move to a position close to the flap, and the culture plate can be taken out by opening the flap by the handle. There is no need to open the incubator for a long time. The driving mechanism is used for taking and placing. The flap is closed during the whole process, which makes the environment inside the incubator more stable. At the same time, there is no need for manual searching, which greatly reduces the possibility of cell contamination and makes the detection effect more accurate.

[0008] Furthermore, a display screen is fixedly connected to the incubator, a plurality of buttons are fixedly connected to the incubator near the display screen, a plurality of baffles are fixedly connected inside the incubator, a side panel is fixedly connected to one side of the incubator, a plurality of strip holes are formed on the side panel, and a plurality of foot pads are fixedly connected to the lower surface of the incubator.

[0009] Through the above technical solution, the baffle can isolate the culture dish from the driving mechanism above, preventing debris generated by the driving mechanism from falling into the culture dish during operation.

[0010] Furthermore, the driving mechanism includes a first motor fixedly connected in the incubator, a plurality of sliding rods are fixedly connected in the incubator, a first screw rod is fixedly connected to the output end of the first motor, a movable frame is slidably connected to the sliding rod, a second motor is fixedly connected to one side of the movable frame, and the second motor passes through a sliding connection bar hole.

[0011] Through the above technical solution, the first motor drives the first screw rod to rotate, the second motor drives the second screw rod to rotate, and the first screw rod cooperates with the moving frame thread, thereby driving the moving frame to move along the sliding rod.

[0012] Furthermore, the first screw rod passes through a threaded connection to the moving frame, an output end of the second motor is fixedly connected to a second screw rod, and a sliding groove is provided on an upper surface of the moving frame.

[0013] Through the above technical solution, the second screw rod cooperates with the moving block thread, thereby driving the moving block to move along the sliding groove.

[0014] Furthermore, the movable support plate includes a movable block which is threadedly connected to a second screw rod, a slider is fixedly connected to the lower surface of the movable block, the slider and the slide groove slide in cooperation with each other, both sides of the movable block are fixedly connected to a cylinder, the output end of the cylinder is fixedly connected to a lifting support plate, the upper surface of the lifting support plate is fixedly connected to an electromagnet, and the edge of the upper surface of the lifting support plate is chamfered.

[0015] Through the above technical solution, the culture plate can be adsorbed by the electromagnet to make the culture plate more stable. At the same time, the chamfer setting of the lifting support plate can make the culture plate more convenient to remove, and the cylinder can push the lifting support plate to rise or fall.

[0016] Furthermore, a plurality of first grooves are provided in the fixing sleeve, a first spring is fixedly connected in the first groove, one end of the first spring away from the first groove is fixedly connected to the wedge block, a second groove is provided on the lower surface of the fixing sleeve, a second spring is fixedly connected in the second groove, a sleeve is fixedly connected at the lower end of the second spring, and the sleeve is made of silicone.

[0017] Through the above technical solution, the truncated table is inserted into the fixed sleeve, at which time the truncated table pushes away the wedge block, and then the wedge block enters the position between the truncated table and the wedge-shaped ring under the push of the first spring to clamp the truncated table.

[0018] Furthermore, a plurality of culture dishes are fixedly connected to the upper surface of the base, the fixed column, thin rod, frustum, wedge-shaped ring and fixed sleeve are all concentrically arranged, an iron block is fixedly connected inside the base, the upper surface of the frustum is inclined, and the lower surface of the wedge-shaped ring is inclined.

[0019] Through the above technical solution, the lifting support plate moves to the bottom of the culture plate and rises to lift the culture plate. At this time, the wedge-shaped ring is equipped with a fixed column to lift and push the wedge block, and the electromagnet is started to adsorb and fix the culture plate. The lifting support plate descends, and under the joint action of the second spring pushing the sleeve, the culture plate moves downward. At this time, the wedge-shaped ring pushes the wedge block through the inclined surface of the lower surface, so that the wedge block cannot be stuck on the round table, and the culture plate will not be stuck by the fixed sleeve.

[0020] Furthermore, the fixing sleeve and the base correspond one to one, the diameter of the fixing column is larger than the thin rod, and the diameter of the wedge-shaped collar is larger than the diameter of the frustum.

[0021] The beneficial effects of the present invention are as follows: (1) The present invention uses a driving mechanism and a culture mechanism. When the culture plate needs to be taken out, the driving mechanism drives the movable support plate to move to the bottom of the culture plate, and then the movable support plate is lifted up to separate the culture plate from the fixed frame. Then, driven by the driving mechanism, the movable support plate drives the culture plate to move to a position close to the flap. The culture plate can be taken out by opening the flap by the handle. There is no need to open the incubator for a long time. The drive mechanism is used to take and place the culture plate. The flap is closed during the whole process, making the environment inside the incubator more stable. At the same time, no manual search is required, which greatly reduces the possibility of cell contamination and makes the detection effect more accurate. (2) The present invention uses an electromagnet on the movable support plate and a second spring and a sleeve in the fixed sleeve. When the culture plate is fixed on the support plate by the electromagnet and when the culture plate is pushed on the fixed frame by the sleeve, the engagement between the truncated cone and the wedge block is tighter, avoiding the shaking of the culture plate, making the culture plate more stable whether on the mobile or fixed frame, and avoiding the cells in the culture dish from spilling out due to shaking. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is the overall structural diagram of the present invention;

[0023] Figure 2 It is the internal structure diagram of the present invention;

[0024] Figure 3 It is a rear side internal structure diagram of the present invention;

[0025] Figure 4 It is a structural diagram of the driving mechanism and the cultivation mechanism of the present invention;

[0026] Figure 5 is a three-dimensional diagram of the driving mechanism of the present invention;

[0027] Figure 6 It is a structural diagram of a movable support plate of the present invention;

[0028] Figure 7 is a stereoscopic diagram of the culture mechanism of the present invention;

[0029] Figure 8 is an exploded view of the culture mechanism of the present invention;

[0030] Fig. 9 is a cross-sectional view of a culture plate of the present invention;

[0031] Fig.10 yes Fig. 9 Enlarged view of point A.

[0032] 1. Incubator; 2. Foot pad; 3. Flip plate; 4. Handle; 5. Driving mechanism; 51. First motor; 52. First screw rod; 53. Slide rod; 54. Moving frame; 55. Slide groove; 56. Second screw rod; 57. Second motor; 6. Moving support plate; 61. Moving block; 62. Cylinder; 63. Lifting support plate; 64. Slide block; 65. Electromagnet; 7. Incubator; 71. Fixed frame; 72. Fixed sleeve; 73. First groove; 74. First spring; 75. Wedge block; 76. Second groove; 77. Second spring; 78. Sleeve; 8. Incubator plate; 81. Base; 82. Fixed column; 83. Thin rod; 84. Cone; 85. Wedge-shaped collar; 86. Incubator dish; 9. Display screen; 10. Button; 11. Side panel; 12. Strip hole; 13. Baffle. DETAILED DESCRIPTION

[0033] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0034] like Figure 1-Figure 10 As shown, a cell detection device of this embodiment includes an incubator 1, one side of the incubator 1 is rotatably connected to a plurality of flaps 3, and a handle 4 is fixedly connected to each of the plurality of flaps 3, and the flaps 3 are opened by the handle 4.

[0035] A display screen 9 is fixedly connected to the incubator 1, a plurality of buttons 10 are fixedly connected to the incubator 1 near the display screen 9, a plurality of baffles 13 are fixedly connected inside the incubator 1, a side panel 11 is fixedly connected to one side of the incubator 1, a plurality of strip holes 12 are formed on the side panel 11, a plurality of foot pads 2 are fixedly connected to the lower surface of the incubator 1, and the use of the baffles 13 can prevent debris generated during the movement of the driving mechanism 5 from falling into the culture dish 86.

[0036] like Figure 3-Figure 6As shown, a driving mechanism 5 is fixedly connected in the incubator 1, and the driving mechanism 5 includes a first motor 51 fixedly connected in the incubator 1, and a plurality of sliding rods 53 are fixedly connected in the incubator 1. The output end of the first motor 51 is fixedly connected to a first screw rod 52, and a moving frame 54 is slidably connected to the sliding rod 53. A second motor 57 is fixedly connected to one side of the moving frame 54, and the second motor 57 passes through the sliding connection bar hole 12, and the first screw rod 52 passes through the threaded connection moving frame 54, and the output end of the second motor 57 is fixedly connected to a second screw rod 56. A slide groove 55 is provided on the upper surface of the movable frame 54, the first motor 51 drives the first screw rod 52 to rotate, the second motor 57 drives the second screw rod 56 to rotate, the first screw rod 52 is threadedly matched with the movable frame 54, thereby driving the movable frame 54 to move along the slide rod 53, and the second screw rod 56 is threadedly matched with the movable block 61, thereby driving the movable block 61 to move along the slide groove 55. Driven by the first motor 51 and the second motor 57, the movable block 61 can move freely with two degrees of freedom in the plane, thereby driving the lifting support plate 63 to move freely.

[0037] A movable support plate 6 is provided on the driving mechanism 5. The movable support plate 6 includes a movable block 61 which is threadedly connected to the second screw rod 56. A slider 64 is fixedly connected to the lower surface of the movable block 61. The slider 64 and the slide groove 55 slide with each other. Cylinders 62 are fixedly connected to both sides of the movable block 61. The output end of the cylinder 62 is fixedly connected to a lifting support plate 63. The upper surface of the lifting support plate 63 is fixedly connected to an electromagnet 65. The upper surface edge of the lifting support plate 63 is chamfered. The cylinder 62 pushes the lifting support plate 63 to rise, and the electromagnet 65 is started to adsorb the base 81.

[0038] like Figure 7-Figure 10 As shown, a plurality of culture racks 7 are fixedly connected in the incubator 1, and the culture racks 7 include a fixed rack 71 fixedly connected in the incubator 1, a plurality of fixed sleeves 72 are fixedly connected to the lower surface of the fixed rack 71, a wedge 75 is slidably connected in the fixed sleeve 72, and a round table 84 is inserted into the fixed sleeve 72. At this time, the round table 84 pushes away the wedge 75, and then the wedge 75 enters the position between the round table 84 and the wedge ring 85 under the push of the first spring 74, and the round table 84 is clamped.

[0039] A plurality of first grooves 73 are provided in the fixing sleeve 72, and a first spring 74 is fixedly connected in the first groove 73. One end of the first spring 74 away from the first groove 73 is fixedly connected to the wedge block 75. A second groove 76 is provided on the lower surface of the fixing sleeve 72, and a second spring 77 is fixedly connected in the second groove 76. A sleeve 78 is fixedly connected at the lower end of the second spring 77. The sleeve 78 is made of silicone. The culture plate 8 is lifted up. At this time, the wedge-shaped ring 85 is lifted up by the fixing column 82 and pushes away the wedge block 75. The electromagnet 65 is started to adsorb and fix the culture plate 8, and the lifting support plate 63 descends. Under the joint action of the second spring 77 pushing the sleeve 78, the culture plate 8 moves downward. At this time, the wedge-shaped ring 85 pushes away the wedge block 75 through the inclined surface of the lower surface, so that the wedge block 75 cannot get stuck on the round table 84, so that the culture plate 8 will not be stuck by the fixing sleeve 72.

[0040] A number of culture plates 8 are arranged on the culture rack 7. The culture plates 8 include a base 81 arranged below the fixed sleeve 72. A fixed column 82 is fixedly connected to the base 81. A thin rod 83 is fixedly connected to the upper end of the fixed column 82. A round table 84 is fixedly connected to the upper end of the thin rod 83. A wedge-shaped ring 85 is slidably connected to the outside of the thin rod 83. A number of culture dishes 86 are fixedly connected to the upper surface of the base 81. The fixed column 82, the thin rod 83, the round table 84, the wedge-shaped ring 85 and the fixed sleeve 72 are all concentrically arranged. An iron block is fixedly connected inside the base 81. The upper surface of the round table 84 is inclined, and the lower surface of the wedge-shaped ring 85 is inclined.

[0041] The fixing sleeve 72 and the base 81 correspond to each other one by one. The diameter of the fixing column 82 is larger than the thin rod 83 , and the diameter of the wedge-shaped collar 85 is larger than the diameter of the truncated cone 84 .

[0042] The working principle of this embodiment is as follows: the first motor 51 drives the first screw rod 52 to rotate, the second motor 57 drives the second screw rod 56 to rotate, the first screw rod 52 is threadedly matched with the moving frame 54, thereby driving the moving frame 54 to move along the slide rod 53, and the second screw rod 56 is threadedly matched with the moving block 61, thereby driving the moving block 61 to move along the slide groove 55. Driven by the first motor 51 and the second motor 57, the moving block 61 can move freely with two degrees of freedom in a plane, thereby driving the lifting support plate 63 to move freely.

[0043] When cells need to be cultured, the staff adds cells and culture fluid into the culture dish 86 on the base 81, and then controls the driving mechanism 5 through the button 10, drives the lifting plate 63 to move to a position close to the flap 3 through the driving mechanism 5, opens the flap 3 through the handle 4 and places the culture plate 8 on the lifting plate 63, and then starts the electromagnet 65 to adsorb the base 81.

[0044] Then, the culture plate 8 is moved to a predetermined position through the driving mechanism 5, and the cylinder 62 pushes the lifting support plate 63 to rise, so that the round table 84 is inserted into the fixed sleeve 72. At this time, the round table 84 pushes away the wedge block 75, and then the wedge block 75 enters the position between the round table 84 and the wedge ring 85 under the push of the first spring 74, and clamps the round table 84, thereby fixing the culture plate 8 on the fixed frame 71, and then the electromagnet 65 is closed, and the lifting support plate 63 descends and resets.

[0045] When the culture plate 8 needs to be taken out, the lifting support plate 63 moves to the bottom of the culture plate 8 and rises to lift the culture plate 8. At this time, the wedge-shaped ring 85 is lifted by the fixed column 82 and pushes away the wedge block 75. The electromagnet 65 is started to adsorb and fix the culture plate 8. The lifting support plate 63 descends. Under the joint action of the second spring 77 pushing the sleeve 78, the culture plate 8 moves downward. At this time, the wedge-shaped ring 85 pushes away the wedge block 75 through the inclined surface of the lower surface, so that the wedge block 75 cannot be stuck on the round table 84, so that the culture plate 8 will not be stuck by the fixed sleeve 72. Afterwards, driven by the driving mechanism 5, the culture plate 8 moves to a position close to the flip plate 3, and the staff opens the flip plate 3 through the handle 4 to take out the culture plate 8.

[0046] The above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention.

Claims

1. A cell detection device, comprising an incubator (1), characterized in that: A driving mechanism (5) is fixedly connected inside the incubator (1), a movable support plate (6) is arranged on the driving mechanism (5), a plurality of culture racks (7) are fixedly connected inside the incubator (1), a plurality of culture plates (8) are arranged on the culture racks (7), a plurality of flaps (3) are rotatably connected to one side of the incubator (1), and a handle (4) is fixedly connected to each of the flaps (3); The culture rack (7) comprises a fixed frame (71) fixedly connected to the culture box (1), a plurality of fixed sleeves (72) fixedly connected to the lower surface of the fixed frame (71), a wedge (75) slidably connected inside the fixed sleeve (72), and the culture plate (8) comprises a base (81) arranged below the fixed sleeve (72), a fixed column (82) fixedly connected to the base (81), a thin rod (83) fixedly connected to the upper end of the fixed column (82), a round table (84) fixedly connected to the upper end of the thin rod (83), and a wedge-shaped collar (85) slidably connected to the outside of the thin rod (83).

2. A cell detection device according to claim 1, characterized in that: The incubator (1) is fixedly connected to a display screen (9); a plurality of buttons (10) are fixedly connected to the incubator (1) at a position close to the display screen (9); a plurality of baffles (13) are fixedly connected inside the incubator (1); a side plate (11) is fixedly connected to one side of the incubator (1); a plurality of strip holes (12) are formed on the side plate (11); and a plurality of foot pads (2) are fixedly connected to the lower surface of the incubator (1).

3. A cell detection device according to claim 2, characterized in that: The driving mechanism (5) comprises a first motor (51) fixedly connected in the incubator (1), a plurality of sliding rods (53) fixedly connected in the incubator (1), an output end of the first motor (51) fixedly connected to a first screw rod (52), a moving frame (54) slidably connected to the sliding rod (53), a second motor (57) fixedly connected to one side of the moving frame (54), and the second motor (57) passes through the sliding connection bar hole (12).

4. A cell detection device according to claim 3, characterized in that: The first screw rod (52) is threadedly connected to the moving frame (54); the output end of the second motor (57) is fixedly connected to the second screw rod (56); and a sliding groove (55) is provided on the upper surface of the moving frame (54).

5. A cell detection device according to claim 4, characterized in that: The movable support plate (6) comprises a movable block (61) threadedly connected to a second screw rod (56); a slider (64) is fixedly connected to the lower surface of the movable block (61); the slider (64) and the slide groove (55) are slidably matched with each other; both sides of the movable block (61) are fixedly connected to a cylinder (62); the output end of the cylinder (62) is fixedly connected to a lifting support plate (63); the upper surface of the lifting support plate (63) is fixedly connected to an electromagnet (65); and the upper surface edge of the lifting support plate (63) is chamfered.

6. A cell detection device according to claim 1, characterized in that: The fixing sleeve (72) is provided with a plurality of first grooves (73), a first spring (74) is fixedly connected to the first groove (73), one end of the first spring (74) away from the first groove (73) is fixedly connected to the wedge block (75), a second groove (76) is provided on the lower surface of the fixing sleeve (72), a second spring (77) is fixedly connected to the second groove (76), a sleeve (78) is fixedly connected to the lower end of the second spring (77), and the sleeve (78) is made of silicone.

7. A cell detection device according to claim 6, characterized in that: A plurality of culture dishes (86) are fixedly connected to the upper surface of the base (81); the fixed column (82), the thin rod (83), the truncated cone (84), the wedge-shaped collar (85) and the fixed sleeve (72) are all arranged concentrically; an iron block is fixedly connected inside the base (81); the upper surface of the truncated cone (84) is arranged obliquely; and the lower surface of the wedge-shaped collar (85) is arranged obliquely.

8. A cell detection device according to claim 7, characterized in that: The fixing sleeve (72) and the base (81) correspond one to one, the diameter of the fixing column (82) is larger than the thin rod (83), and the diameter of the wedge-shaped collar (85) is larger than the diameter of the truncated cone (84).

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