Mesenchymal stem cell culture device

Abstract

The invention discloses a mesenchymal stem cell culture device, and relates to the field of stem cell culture devices.The mesenchymal stem cell culture device comprises a culture bottle, the surface of the culture bottle is fixedly connected with an anti-skid sleeve, the inner wall of the culture bottle is fixedly connected with a feeding barrel, the surface of the feeding barrel is in threaded connection with a nut, and the surface of the culture bottle is provided with a positioning hole; a supporting seat is fixedly connected to the surface of the culture bottle, an observation window is fixedly connected to the inner wall of the culture bottle, and lightweight holes are formed in the surface of the supporting seat. When the culture bottle is vertically placed through the balancing weight, the gravity center of the culture bottle is kept to be located below the culture bottle, the phenomenon that the culture bottle turns on one side during operation is prevented, the culture bottle is attracted and fixed to an external metal workbench through the multiple magnetic attraction blocks, and the stability of the culture bottle during operation is further kept; a plurality of arc plates are used for clamping and positioning objects in the positioning holes, so that the culture bottle is prevented from moving in an environment without magnetic attraction of the magnetic attraction blocks.

Description

Technical Field

[0001] The present invention relates to the field of stem cell culture devices, and in particular to a mesenchymal stem cell culture device. Background Art

[0002] Mesenchymal stem cells are a type of pluripotent stem cells that have all the common properties of stem cells, namely, self-renewal and multidirectional differentiation capabilities. They are also the most widely used in clinical applications. When used in combination with hematopoietic stem cells, they can improve the success rate of transplantation and accelerate hematopoietic reconstruction. When patients receive high-dose chemotherapy, the infusion of mesenchymal stem cells together with hematopoietic stem cells can significantly accelerate the recovery time of patients' blood cells, and is safe and has no adverse reactions. Mesenchymal stem cells exist not only in the bone marrow, but also in skeletal muscle, periosteum, and trabecular bone.

[0003] Common mesenchymal stem cell culture media include DMEM / F12, α-MEM, RPMI1640, etc. These culture media contain appropriate amounts of nutrients, growth factors, serum and other elements to provide the cells with the necessary growth environment. The inventor believes that there are certain problems. On the one hand, the stability of traditional culture medium bottles is poor when injecting and culturing cells, and the culture device is easily shaken when operating the culture device. On the other hand, general culture bottles can only culture cells in a single layer, which makes the cell culture attachment area in the culture bottle low and inconvenient for culture. Summary of the invention

[0004] The object of the present invention is to provide a mesenchymal stem cell culture device to solve the problems raised in the above-mentioned background technology. On the one hand, the traditional culture medium bottle has poor stability when injecting and culturing cells, and the culture device is easily shaken when operating the culture device. On the other hand, the general culture bottle can only culture cells in a single layer, so that the culture attachment area of ​​the cells in the culture bottle is low, which is not convenient for culture.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a mesenchymal stem cell culture device, comprising a culture bottle, a non-slip sleeve is fixedly connected to the surface of the culture bottle, a feed barrel is fixedly connected to the inner wall of the culture bottle, a nut is threadedly connected to the surface of the feed barrel, a positioning hole is opened on the surface of the culture bottle, a support seat is fixedly connected to the surface of the culture bottle, an observation window is fixedly connected to the inner wall of the culture bottle, a light-weight hole is opened on the surface of the support seat, and a stable ventilation mechanism is fixedly connected to the inner wall of the culture bottle;

[0006] The stable ventilation mechanism includes an upper plate, a middle plate and a bottom plate fixedly connected to the inner wall of the culture bottle, the surfaces of the upper plate, the middle plate and the bottom plate are each provided with a plurality of through holes, the surface of the culture bottle is fixedly connected to a friction sleeve, the surface of the culture bottle is fixedly connected to a plurality of magnetic blocks, the surface of the culture bottle is fixedly connected to a counterweight block, and the counterweight block is installed at the center position of the plurality of magnetic blocks.

[0007] The inner wall of the positioning hole is fixedly connected with a telescopic rod, one end of the telescopic rod is fixedly connected with an arc plate, and a spring is sleeved on the surface of the telescopic rod.

[0008] An isolation plate is fixedly connected to the inner wall of the culture bottle, a sealing ring is fixedly connected to the surface of the isolation plate, and a plurality of air holes are opened on the upper surface of the isolation plate.

[0009] The inner wall of the culture bottle is rotatably connected with a pressing cover, the inner wall of the culture bottle is provided with a card slot, one side of the pressing cover is fixedly connected with a plastic card head, and the plastic card head is card-engaged with the card slot.

[0010] The inner wall of the culture bottle is fixedly connected with a water tank, the inner wall of the water tank is fixedly connected with a downpipe, and the inner wall of the downpipe is fixedly connected with a threaded cap.

[0011] The surface of the culture bottle is fixedly connected with a micro electric telescopic rod, the output end of the micro electric telescopic rod is fixedly connected with a sealing plate, the surface of the sealing plate is slidably connected to the inner wall of the culture bottle, and the lower surface of the sealing plate is in contact with the inner bottom wall of the water tank.

[0012] The inner bottom wall of the water tank is provided with a plurality of water dripping holes, and the upper plate, the middle plate and the bottom plate are arranged in sequence from top to bottom.

[0013] A first avoidance hole and a second avoidance hole are formed on the upper surface of the upper plate, and a third avoidance hole is formed on the upper surface of the middle plate. The third avoidance hole corresponds to the position of the first avoidance hole.

[0014] In summary, the technical effects and advantages of the present invention are as follows:

[0015] 1. In the present invention, the counterweight block allows the center of gravity of the culture bottle to be kept below the culture bottle when the culture bottle is placed vertically, preventing the culture bottle from tipping over during operation. The culture bottle is fixed to an external metal workbench by multiple magnetic blocks, further maintaining stability during operation. Multiple arc plates clamp and position objects in the positioning holes, preventing the culture bottle from moving in an environment without magnetic blocks.

[0016] 2. In the present invention, multiple drip holes are used to supply nutrients to the upper plate, the middle plate and the bottom plate. The first avoidance hole and the second avoidance hole avoid the nutrients dripping from the multiple rows of drip holes, so that they drip onto the middle plate, pass through the first avoidance hole, and then drip onto the bottom plate through the third avoidance hole. Nutrients are supplied to the cells on the upper plate, the middle plate and the bottom plate. By setting the upper plate, the middle plate and the bottom plate, the cells are cultured in layers. This has the advantage of increasing the culture attachment area of ​​the cells in the culture bottle.

[0017] 3. In the present invention, the plastic card head has toughness and can be deformed and sealed during the card connection with the card slot. The advantage of this is that it can be sealed at the same time as the nut, which is convenient for temporary carrying of the culture bottle and prevents the cells cultured inside from flowing out. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0019] Figure 1 It is a schematic diagram of the three-dimensional structure of an embodiment of the present invention;

[0020] Figure 2 It is a schematic diagram of the three-dimensional structure of the friction sleeve in an embodiment of the present invention;

[0021] Figure 3 This is a schematic cross-sectional view of a culture bottle in an embodiment of the present invention;

[0022] Figure 4 For the embodiment of the present invention Figure 3 A schematic diagram of the enlarged structure at A in the middle;

[0023] Figure 5 It is a schematic cross-sectional structural diagram of a water tank in an embodiment of the present invention;

[0024] Figure 6 Schematic diagram of the three-dimensional structure of the drip hole in the embodiment of the present invention;

[0025] Figure 7 It is a schematic diagram of the three-dimensional structure of the upper plate, the middle plate and the bottom plate in an embodiment of the present invention.

[0026] In the figure: 1. culture bottle; 2. nut; 3. push cover; 4. support seat; 5. observation window; 6. anti-slip cover; 7. micro electric telescopic rod; 8. positioning hole; 9. telescopic rod; 10. spring; 11. arc plate; 12. friction sleeve; 13. counterweight; 14. threaded cap; 15. downpipe; 16. sealing plate; 17. water tank; 18. upper plate; 19. middle plate; 20. bottom plate; 21. isolation plate; 22. air vent; 23. sealing ring; 24. plastic card head; 25. through hole; 26. third avoidance hole; 27. second avoidance hole; 28. first avoidance hole; 29. ​​card slot; 30. feed barrel; 31. lightweight hole; 32. magnetic suction block; 33. drip hole. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Example: Reference Figure 1-Figure 7 A mesenchymal stem cell culture device shown includes a culture bottle 1, a non-slip cover 6 is fixedly connected to the surface of the culture bottle 1, a feed barrel 30 is fixedly connected to the inner wall of the culture bottle 1, a nut 2 is threadedly connected to the surface of the feed barrel 30, a positioning hole 8 is opened on the surface of the culture bottle 1, a support seat 4 is fixedly connected to the surface of the culture bottle 1, an observation window 5 is fixedly connected to the inner wall of the culture bottle 1, a light hole 31 is opened on the surface of the support seat 4, and a stable ventilation mechanism is fixedly connected to the inner wall of the culture bottle 1;

[0029] The stable ventilation mechanism includes an upper plate 18, a middle plate 19 and a bottom plate 20 fixedly connected to the inner wall of the culture bottle 1, and a plurality of through holes 25 are opened on the surfaces of the upper plate 18, the middle plate 19 and the bottom plate 20. A friction sleeve 12 is fixedly connected to the surface of the culture bottle 1, a plurality of magnetic blocks 32 are fixedly connected to the surface of the culture bottle 1, and a counterweight block 13 is fixedly connected to the surface of the culture bottle 1. The counterweight block 13 is installed at the center position of the plurality of magnetic blocks 32.

[0030] By means of the above structure, the nut 2 is provided to seal the opening position of the feed barrel 30. By providing the support seat 4, when the culture bottle 1 is placed horizontally, the lowest surface height of the support seat 4 can maintain the left and right balance of the culture bottle 1, and support the culture bottle 1 to be placed horizontally. By providing the anti-slip sleeve 6, the contact friction of the user is increased. By providing the positioning hole 8, it is convenient to fix the culture bottle 1 as a whole through the positioning hole 8 when operating the culture bottle 1. By providing the lightweight hole 31, the weight of the support seat 4 is reduced. By providing the observation window 5, the material of the observation window 5 is transparent. The flask 1 is made of a material and is used to observe changes in the situation inside the culture bottle 1. The upper plate 18, the middle plate 19 and the bottom plate 20 are provided to culture cells in layers. The multiple through holes 25 are provided to allow air to flow through the multiple through holes 25. The counterweight 13 is provided to keep the center of gravity below the culture bottle 1 when the culture bottle 1 is placed vertically, thereby preventing the culture bottle 1 from tipping over during operation. The multiple magnetic blocks 32 are provided to fix the culture bottle 1 to an external metal workbench through adsorption, thereby further maintaining the stability of the culture bottle 1 during operation.

[0031] As a preferred implementation in this embodiment, a telescopic rod 9 is fixedly connected to the inner wall of the positioning hole 8, an arc plate 11 is fixedly connected to one end of the telescopic rod 9, and a spring 10 is sleeved on the surface of the telescopic rod 9.

[0032] By setting the telescopic rod 9 and fixing the arc plate 11, multiple arc plates 11 can clamp and position the object in the positioning hole 8. In the absence of magnetic attraction of the magnetic block 32, the culture bottle 1 is prevented from moving. By setting the spring 10, the multiple arc plates 11 are driven to clamp in the middle.

[0033] As a preferred implementation in this embodiment, an isolation plate 21 is fixedly connected to the inner wall of the culture bottle 1, a sealing ring 23 is fixedly connected to the surface of the isolation plate 21, and a plurality of air holes 22 are opened on the upper surface of the isolation plate 21.

[0034] By providing a plurality of air holes 22, after the culture bottle 1 is placed in the culture device, ventilation is performed through the plurality of air holes 22 so that the cells can adapt to the air environment in the culture device. By providing a sealing ring 23, when the culture bottle 1 is used, the sealing ring 23 is pressed into contact with the surface of the pressing cover 3 to perform sealing. The advantage of this is that it can be sealed simultaneously with the nut 2, making it convenient to temporarily carry the culture bottle 1 so that the cells cultured inside will not flow out.

[0035] As a preferred implementation in this embodiment, the inner wall of the culture bottle 1 is rotatably connected with a pressing cover 3, the inner wall of the culture bottle 1 is provided with a card slot 29, and one side of the pressing cover 3 is fixedly connected with a plastic clamp head 24, which is engaged with the card slot 29.

[0036] By providing the card slot 29 , space is provided for the plastic card head 24 to engage with the card slot 29 . By providing the plastic card head 24 , the plastic card head 24 has toughness and can be deformed during the engagement with the card slot 29 .

[0037] As a preferred implementation in this embodiment, the inner wall of the culture bottle 1 is fixedly connected to a water tank 17 , the inner wall of the water tank 17 is fixedly connected to a downpipe 15 , and the inner wall of the downpipe 15 is fixedly connected to a threaded cap 14 .

[0038] By setting up a water tank 17, nutrients or growth factors for cell growth are stored, and by setting up a threaded cap 14, the top of the downcomer 15 is sealed. By setting up the downcomer 15, nutrients or growth factors can be added to the water tank 17 through the downcomer 15.

[0039] As a preferred implementation manner in this embodiment, a micro electric telescopic rod 7 is fixedly connected to the surface of the culture bottle 1, and a sealing plate 16 is fixedly connected to the output end of the micro electric telescopic rod 7. The surface of the sealing plate 16 is slidingly connected to the inner wall of the culture bottle 1, and the lower surface of the sealing plate 16 is in contact with the inner bottom wall of the water tank 17.

[0040] By setting up the micro electric telescopic rod 7 and starting the micro electric telescopic rod 7 , the output end of the micro electric telescopic rod 7 drives the sealing plate 16 to slide in the water tank 17 to adjust the position, thereby changing the relative position of the sealing plate 16 and the plurality of drip holes 33 .

[0041] In this embodiment, a plurality of drip holes 33 are provided on the inner bottom wall of the water tank 17, and the upper plate 18, the middle plate 19 and the bottom plate 20 are arranged in sequence from top to bottom.

[0042] By setting a plurality of drip holes 33, nutrients are supplied to the upper plate 18, the middle plate 19 and the bottom plate 20. By setting the upper plate 18, the middle plate 19 and the bottom plate 20 in sequence from top to bottom, cells are cultured in layers. This has the advantage of increasing the culture attachment area of ​​the cells in the culture bottle 1.

[0043] As a preferred implementation in this embodiment, a first avoidance hole 28 and a second avoidance hole 27 are opened on the upper surface of the upper plate 18, and a third avoidance hole 26 is opened on the upper surface of the middle plate 19, and the position of the third avoidance hole 26 corresponds to that of the first avoidance hole 28.

[0044] By setting the first avoidance hole 28 and the second avoidance hole 27, the nutrients dripping from the multiple rows of drip holes 33 are avoided, so that they drip onto the middle plate 19, pass through the first avoidance hole 28, and then drip onto the bottom plate 20 through the third avoidance hole 26, thereby supplying nutrients to the cells on the upper plate 18, the middle plate 19 and the bottom plate 20.

[0045] The working principle of the present invention is: a mesenchymal stem cell culture device, when culturing cells in a culture bottle 1, it is necessary to turn the nut 2 to open the feed barrel 30, and the operator injects the cells through the injection test tube and attaches them to the surfaces of the upper plate 18, the middle plate 19 and the bottom plate 20. At this time, the operator can choose to place it vertically or horizontally for injection operation. When the culture bottle 1 is placed horizontally, the lowest surface height of the support seat 4 can maintain the left and right balance of the culture bottle 1 and support the culture bottle 1 to be placed horizontally. When the culture bottle 1 is placed vertically, the counterweight block 13 keeps the center of gravity below the culture bottle 1 when the culture bottle 1 is placed vertically, so as to prevent the culture bottle 1 from being moved during operation. , the phenomenon of overturning occurs. By setting a plurality of magnetic blocks 32, the plurality of magnetic blocks 32 are fixed by adsorption with the external metal workbench to further maintain the stability of the culture bottle 1 during operation. At the same time, the external tools can be inserted into the positioning hole 8, and the positioning stability is maintained by the clamping of the plurality of arc plates 11. After the injection is completed, the operator can turn the nut 2. In the process of injecting cells, the surface of the pressing cover 3 always squeezes the surface of the sealing ring 23 to maintain stability and prevent the cells from flowing out, avoiding the traditional method of directly opening a plurality of ventilation holes on the nut 2. The advantage of this is that it can be sealed at the same time as the nut 2, which is convenient for temporary carrying of the culture bottle 1, so that the cells cultured inside will not Before sending the culture bottle 1 to the external culture equipment, open the threaded cap 14, inject nutrient solution or growth factor into the downpipe 15 and seal it. When sending the culture bottle 1 to the external culture equipment, it is necessary to push the plastic clamp 24, which has toughness and can be bent and deformed. At this time, the plastic clamp 24 is pushed out from the card slot 29, so that the multiple air holes 22 are ventilated with the air in the external culture equipment. After a certain period of culture, through the observation window 5, when it is necessary to provide nutrition to the cells on the upper plate 18, the middle plate 19 and the bottom plate 20, start the micro-electric telescopic rod 7, and its output end drives the sealing plate 16 to slide in the water tank 17 to position. The position is adjusted to change the relative position of the sealing plate 16 and the multiple drip holes 33, and the nutrients drip from the water tank 17 through the multiple drip holes 33 to supply nutrients to the upper plate 18, the middle plate 19 and the bottom plate 20. The first avoidance hole 28 and the second avoidance hole 27 avoid the nutrients dripping from the multiple rows of drip holes 33, so that they drip on the middle plate 19, pass through the first avoidance hole 28, and then drip on the bottom plate 20 through the third avoidance hole 26 to supply nutrients to the cells on the upper plate 18, the middle plate 19 and the bottom plate 20. The above structure can prevent the culture bottle 1 from tipping over when operating, and at the same time, it can be observed that nutrients are provided to the cells growing inside without destroying the culture environment.

[0046] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A mesenchymal stem cell culture device, comprising a culture bottle (1), characterized in that: The surface of the culture bottle (1) is fixedly connected with an anti-slip sleeve (6), the inner wall of the culture bottle (1) is fixedly connected with a feed barrel (30), the surface of the feed barrel (30) is threadedly connected with a nut (2), the surface of the culture bottle (1) is provided with a positioning hole (8), the surface of the culture bottle (1) is fixedly connected with a support seat (4), the inner wall of the culture bottle (1) is fixedly connected with an observation window (5), the surface of the support seat (4) is provided with a light-weight hole (31), and the inner wall of the culture bottle (1) is fixedly connected with a stable ventilation mechanism; The stable ventilation mechanism comprises an upper plate (18), a middle plate (19) and a bottom plate (20) fixedly connected to the inner wall of a culture bottle (1); the surfaces of the upper plate (18), the middle plate (19) and the bottom plate (20) are all provided with a plurality of through holes (25); the surface of the culture bottle (1) is fixedly connected to a friction sleeve (12); the surface of the culture bottle (1) is fixedly connected to a plurality of magnetic blocks (32); the surface of the culture bottle (1) is fixedly connected to a counterweight block (13); and the counterweight block (13) is installed at the center position of the plurality of magnetic blocks (32).

2. A mesenchymal stem cell culture device according to claim 1, characterized in that: A telescopic rod (9) is fixedly connected to the inner wall of the positioning hole (8), an arc plate (11) is fixedly connected to one end of the telescopic rod (9), and a spring (10) is sleeved on the surface of the telescopic rod (9).

3. The mesenchymal stem cell culture device according to claim 1, characterized in that: An isolation plate (21) is fixedly connected to the inner wall of the culture bottle (1), a sealing ring (23) is fixedly connected to the surface of the isolation plate (21), and a plurality of air holes (22) are provided on the upper surface of the isolation plate (21).

4. The mesenchymal stem cell culture device according to claim 1, characterized in that: The inner wall of the culture bottle (1) is rotatably connected to a pressing cover (3), and the inner wall of the culture bottle (1) is provided with a clamping groove (29).

5. A mesenchymal stem cell culture device according to claim 4, characterized in that: A plastic clamp (24) is fixedly connected to one side of the pressing cover (3), and the plastic clamp (24) is clamped and matched with the clamping groove (29).

6. The mesenchymal stem cell culture device according to claim 1, characterized in that: The inner wall of the culture bottle (1) is fixedly connected to a water tank (17), the inner wall of the water tank (17) is fixedly connected to a downpipe (15), and the inner wall of the downpipe (15) is fixedly connected to a threaded cap (14).

7. The mesenchymal stem cell culture device according to claim 1, characterized in that: A micro electric telescopic rod (7) is fixedly connected to the surface of the culture bottle (1), and a sealing plate (16) is fixedly connected to the output end of the micro electric telescopic rod (7).

8. The mesenchymal stem cell culture device according to claim 7, characterized in that: The surface of the sealing plate (16) is slidably connected to the inner wall of the culture bottle (1), and the lower surface of the sealing plate (16) is in contact with the inner bottom wall of the water tank (17).

9. The mesenchymal stem cell culture device according to claim 6, characterized in that: The inner bottom wall of the water tank (17) is provided with a plurality of water dripping holes (33), and the upper plate (18), the middle plate (19) and the bottom plate (20) are arranged in sequence from top to bottom.

10. The mesenchymal stem cell culture device according to claim 1, characterized in that: The upper surface of the upper plate (18) is provided with a first avoidance hole (28) and a second avoidance hole (27), and the upper surface of the middle plate (19) is provided with a third avoidance hole (26), wherein the position of the third avoidance hole (26) corresponds to that of the first avoidance hole (28).

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