Bifidobacterium animalis subsp. Lactis B08 probiotic preparation capable of colonizing intestinal function and preparation method of bifidobacterium animalis subsp. Lactis B08 probiotic preparation

Abstract

The invention discloses an animal bifidobacterium subsp. Lactis B08 probiotic preparation with an intestinal colonization function and a preparation method. The animal bifidobacterium subsp. Lactis B08 probiotic preparation is prepared from the following raw materials in parts by weight: 1 part of bifidobacterium subsp. Lactis B08, 3 parts of milk powder, 0.5 part of a yeast extract, 1 part of lactose, 0.5 part of trehalose, 2 parts of microcrystalline cellulose and 0.1 part of a stabilizing agent. The preparation can promote intestinal health and improve the digestive function of animals, mixing equipment is optimally used in the preparation process of the preparation, the feeding position can be closed through the feeding structure after materials are put into the preparation, dust leakage is prevented, the mixing and stirring structure is combined with the overturning and stirring mixing technology, and the production efficiency is improved. The materials can move in multiple directions in the mixing process, so that the contact among the materials is increased, and the mixing uniformity and the mixing efficiency are improved.

Description

Technical Field

[0001] The invention relates to the technical field of biological bacteria preparations, in particular to an animal Bifidobacterium lactis subspecies B08 probiotic preparation with intestinal colonization function and a preparation method thereof. Background Art

[0002] Probiotics can enter the human body and colonize the intestines to form a healthy intestinal bacterial film to prevent the colonization of harmful bacteria. Probiotics produce substances that inhibit or control the growth of intestinal pathogenic microorganisms, rebuild the balance of intestinal flora, activate the immune system and improve immunity. Bifidobacterium lactis subspecies B08 is an important probiotic with multiple health benefits, especially in promoting animal intestinal health and enhancing immune function.

[0003] At present, Chinese patent application number: CN200710059434.9 discloses a probiotic preparation with yogurt fermentation and intestinal colonization functions and a preparation method thereof, wherein the preparation contains probiotics, thermophilic streptococci for fermentation and / or bulgaricus lactobacillus as effective ingredients; the ratio of probiotics to thermophilic streptococci, bulgaricus lactobacillus or one of them is 1×10 6 cfu-5x10 10 cfu:1X10 6 cfu-1X10 10 cfu; the preparation contains 1-80% by weight of the above-mentioned active ingredient; 1-15% by weight of prebiotics; the balance is added with auxiliary materials, and 0.0001%-3% by weight of flavor and 0.0001%-2% by weight of medium-chain triglyceride can also be added.

[0004] However, the existing probiotic preparations for colonizing intestinal function have weak colonization ability, which makes the survival time of the strains in the intestines short, the effect is limited, and there is a lack of obvious immunomodulatory effect, resulting in poor effect in enhancing animal health. In addition, during the preparation process of the probiotic preparation, the powder is easily lifted up and leaked when it is added, and the mixing effect of the powder is poorly uniform, thereby affecting the mixing efficiency. Summary of the invention

[0005] The object of the present invention is to provide an animal Bifidobacterium lactis subspecies B08 probiotic preparation with intestinal colonization function and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0006] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a probiotic preparation of animal Bifidobacterium lactis subsp. lactis with intestinal colonization function, the animal Bifidobacterium lactis subsp. B08 probiotic was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on July 22, 2022, and its classification name is: Bifidobacterium animalis subsp. lactis, and its preservation number is CGMCC NO: 25376. The Bifidobacterium lactis subsp. B08 probiotic preparation comprises the following raw materials by weight: 1 part of Bifidobacterium lactis subsp. B08, 3 parts of milk powder, 0.5 parts of yeast extract, 1 part of lactose, 0.5 parts of trehalose, 2 parts of microcrystalline cellulose and 0.1 parts of stabilizer.

[0007] Preferably, the stabilizer is selected from one or both of gelatin and carrageenan.

[0008] Preferably, the total colony count per unit mass of the Bifidobacterium lactis subspecies B08 is 10 8 -10 10 CFU / g.

[0009] In addition, the present invention also provides a method for preparing an animal Bifidobacterium lactis subspecies B08 probiotic preparation having intestinal colonization function, the preparation method comprising the following steps:

[0010] S1, select Bifidobacterium lactis subsp. B08 strain, inoculate it in a liquid culture medium made of milk powder, yeast extract and lactose, and culture it at 37°C for 24-48 hours until it reaches the logarithmic growth phase;

[0011] S2. Separate the bacteria in the liquid culture medium by centrifugation at 4000-6000 rpm for 10-15 minutes, and collect the precipitated bacteria;

[0012] S3. Wash the bacterial precipitate 2-3 times with sterile saline to remove residual substances in the culture medium;

[0013] S4. Resuspend the washed cells in PBS buffer and adjust the concentration to 10 8 -10 10 CFU / ml;

[0014] S5, adding freeze-drying protective agent trehalose to the bacterial suspension, and then filling the bacterial suspension into freeze-drying bottles, and freeze-drying to prepare a powdered probiotic preparation;

[0015] S6. The freeze-dried probiotic powder is mixed evenly with microcrystalline cellulose and a stabilizer through a mixing device to form a final preparation form, and then the preparation is aseptically packaged using microencapsulation technology to protect the probiotics from the effects of gastric acid and bile salts.

[0016] Preferably, the mixing equipment includes a silo, a support frame fastened to the top side of the front of the silo, a driving motor fastened to the front side of the support frame, a reduction gear box connected to the top output end of the driving motor, an inspection cover hinged to the middle side of the front of the silo, a buckle arranged on the left side of the front of the inspection cover, a fastening bolt inserted and locked on the inner side of the buckle, a feeding structure fixed to the upper right side of the silo, a mixing structure connected to the rear side of the bottom of the reduction gear box, and a discharge port arranged on the bottom side of the left part of the silo, the reduction gear box is installed on the top side of the silo, the rear side of the fastening bolt is rotatably connected to the silo, the mixing structure is arranged on the middle side of the inside of the silo, a switch solenoid valve is arranged on the inner side of the discharge port, and three buckles and three fastening bolts are provided.

[0017] Preferably, the feeding structure includes a silo cover fixed to the silo barrel on the left side, a driving assembly arranged on the upper right side of the rear part of the silo cover, a lifting rod connected to the top side of the front part of the driving assembly, a cover plate rotatably connected to the top side of the rear part of the lifting rod, a support rod rotatably connected to the left part of the bottom side of the cover plate, a support seat rotatably connected to the rear part of the middle and lower side of the support rod, a material tray rotatably connected to the bottom end part of the support rod, and a pad embedded in the upper and middle side of the right side wall of the silo barrel, the cover plate is arranged on the upper right side of the silo cover, the rear side of the support seat is fixed to the silo cover, and the left part of the material tray is provided with an upward inclination angle.

[0018] Preferably, the drive assembly includes a rectangular seat fixed to the bin cover on the front side, a servo motor fastened to the lower right side of the rectangular seat, a worm connected to the top output end of the servo motor, two support blocks arranged on the upper and lower sides of the worm, a worm wheel meshingly transmitted to the left side of the worm, a support frame arranged on the front side of the worm wheel, and a shaft column fixed to the middle side of the front part of the worm wheel, the right side of the support block is fixed to the rectangular seat, the left side of the support frame is fixed to the rectangular seat, the shaft column passes through and rotates on the inner side of the support frame, and the front end of the shaft column is fastened to the lifting rod.

[0019] Preferably, the mixing structure includes a rotating rod connected to the reduction gear box at the top end, a bearing block arranged on the outer surface of the rotating rod, a first stirring assembly connected to the bottom end of the rotating rod, an arc block fixed to the outer side of the bottom of the first stirring assembly, a cross fixedly connected to the inner side of the arc block, a second stirring assembly connected to the bottom side of the arc block, and a positioning column connected to the middle side of the top of the second stirring assembly, the rear side of the bearing block is fixed to the silo, the outer sides of the first stirring assembly, the arc block and the second stirring assembly are all in contact with the inner wall of the silo, the middle side of the bottom of the second stirring assembly is fixed to the silo, the top side of the positioning column is connected to the first stirring assembly, and the first stirring assembly and the second stirring assembly have the same structure and size.

[0020] Preferably, four arc blocks and four crosses are provided, and the upper and lower sides thereof are respectively connected to the first stirring component and the second stirring component.

[0021] Preferably, the first stirring assembly includes a support tube, four rotating frames rotatably connected to the four axial positions of the outer surface of the support tube, a scraper frame fixed to the outside of the rotating frame, three blades rotating through the inner side of the rotating frame, a rotating piece fixedly connected to the side of the blade close to the support tube, a convex ball rod fixedly connected to the end of the rotating piece away from the blade connection, and three guide grooves connected in a stepped manner and arranged on the outer surface of the support tube, the middle side of the bottom of the support tube is fixed to the positioning column, the outer side of the scraper frame is in contact with the inner wall of the silo, and the bottom side of the scraper frame is fixed to the arc block and the cross, and the spherical end of the convex ball rod is inserted and slid into the inner side of the guide groove.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The bifidobacterium lactis subspecies B08 probiotic preparation of the present invention comprises the following raw materials by weight: 1 part of bifidobacterium lactis subspecies B08, 3 parts of milk powder, 0.5 parts of yeast extract, 1 part of lactose, 0.5 parts of trehalose, 2 parts of microcrystalline cellulose and 0.1 parts of a stabilizer, wherein the stabilizer is selected from one or both of gelatin and carrageenan, and the total colony count per unit mass of bifidobacterium lactis subspecies B08 is 10 8 -10 10 CFU / g, the preparation can promote intestinal health and improve the digestive function of animals.

[0024] In the preparation process of the bifidobacterium lactis subspecies B probiotic preparation, the present invention optimizes the use of a mixing device, the feeding place can be closed after the material is put in through the feeding structure to prevent dust leakage, the reduction box drives the mixing structure to move inside the silo through the action of the driving motor to complete the mixing of the powder, the mixing structure is combined with the mixing technology of flipping and stirring, so that the material can move in multiple directions during the mixing process, the contact between the materials is increased to improve the uniformity of the mixing, and the side wall of the silo can be scraped to improve the mixing efficiency of the powder, the buckle and the fastening bolt are both provided with three, the inspection cover can be opened or closed on the silo through the cooperation of the fastening bolt and the buckle, the cleaning and maintenance of the inside of the silo is convenient, and the risk of cross contamination is reduced.

[0025] The invention drives the arc block and the cross to move synchronously when the scraper frame rotates, the spherical end of the convex ball rod is inserted and slid inside the guide groove, and drives the convex ball rod to move in the guide groove of the support tube during the rotation of the rotating frame. When the convex ball rod moves to the lowest side position in the guide groove, the convex ball rod drives the blade to rotate to a vertical state. When the convex ball rod moves to the highest side position in the guide groove, the convex ball rod drives the blade to rotate to a horizontal state, so that the blade performs a flipping action of the rotation position, so as to improve the mixing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a flowchart of the preparation method of the present invention;

[0027] Figure 2 It is a structural schematic diagram of the mixing device of the present invention;

[0028] Figure 3 This is a schematic diagram of the structure inside the silo of the present invention;

[0029] Figure 4 It is a structural schematic diagram of the feeding structure of the present invention;

[0030] Figure 5 It is a schematic diagram of the structure of the driving assembly of the present invention;

[0031] Figure 6 It is a structural schematic diagram of the mixing structure of the present invention;

[0032] Figure 7 It is a schematic structural diagram of the first stirring component of the present invention.

[0033] In the figure: silo-1, cushion frame-2, drive motor-3, reduction box-4, inspection cover-5, buckle-6, fastening bolt-7, feeding structure-8, mixing structure-9, discharge port-10, silo cover-81, drive assembly-82, lifting rod-83, cover plate-84, support rod-85, support-86, material tray-87, cushion block-88, rectangular seat-821, servo motor-822, worm-82 3. Support block 824, worm gear 825, support frame 826, rotating shaft column 827, rotating rod 91, bearing block 92, first stirring assembly 93, arc block 94, cross 95, second stirring assembly 96, positioning column 97, support tube 931, rotating frame 932, scraping frame 933, blade 934, rotating piece 935, convex ball rod 936, guide groove 937. DETAILED DESCRIPTION

[0034] In order to further explain the technical solution of the present invention, it is described in detail below through specific embodiments.

[0035] The present invention provides an animal Bifidobacterium lactis subspecies B08 probiotic preparation with intestinal colonization function. The animal Bifidobacterium lactis subspecies B08 probiotic was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee on July 22, 2022, and its deposit number is CGMCC NO: 25376. The Bifidobacterium lactis subspecies B08 probiotic preparation comprises the following raw materials by weight: 1 part of Bifidobacterium lactis subspecies B08, 3 parts of milk powder, 0.5 parts of yeast extract, 1 part of lactose, 0.5 parts of trehalose, 2 parts of microcrystalline cellulose and 0.1 parts of a stabilizer, wherein the stabilizer is selected from one or both of gelatin and carrageenan, and the total colony count per unit mass of the Bifidobacterium lactis subspecies B08 is 10 8 -10 10CFU / g, the preparation can promote intestinal health and improve the digestive function of animals.

[0036] See also Figure 1-Figure 7 The present invention provides a method for preparing a probiotic preparation of animal Bifidobacterium lactis subspecies B08 with intestinal colonization function, comprising the following steps:

[0037] S1, select Bifidobacterium lactis subsp. B08 strain, inoculate it in a liquid culture medium made of milk powder, yeast extract and lactose, and culture it at 37°C for 24-48 hours until it reaches the logarithmic growth phase;

[0038] S2, separating the bacteria in the liquid culture medium by centrifugation at 6000 rpm for 12 minutes, and collecting the precipitated bacteria;

[0039] S3, wash the bacterial precipitate 3 times with sterile saline to remove residual substances in the culture medium;

[0040] S4. Resuspend the washed cells in PBS buffer and adjust the concentration to 10 9 CFU / ml;

[0041] S5, adding freeze-drying protective agent trehalose to the bacterial suspension, and then filling the bacterial suspension into freeze-drying bottles, and freeze-drying to prepare a powdered probiotic preparation;

[0042] S6. The freeze-dried probiotic powder is mixed evenly with microcrystalline cellulose and carrageenan through a mixing device to form the final preparation form, and then the preparation is aseptically packaged using microencapsulation technology to protect the probiotics from the effects of gastric acid and bile salts.

[0043] The mixing equipment includes a silo 1, a support frame 2 fastened to the top side of the front of the silo 1, a driving motor 3 fastened to the front side of the support frame 2, a reduction gearbox 4 connected to the top output end of the driving motor 3, an inspection cover 5 hinged to the middle side of the front of the silo 1, a buckle 6 arranged on the left side of the front of the inspection cover 5, a fastening bolt 7 inserted and locked on the inner side of the buckle 6, a feeding structure 8 fixed to the upper right side of the silo 1, a mixing structure 9 connected to the bottom rear side of the reduction gearbox 4 and a discharge port 10 arranged on the bottom left side of the silo 1. The reduction gearbox 4 is installed on the top side of the silo 1, and the rear side of the fastening bolt 7 is rotatably connected to the silo 1. The mixing structure 9 is arranged on the middle side of the inside of the silo 1. The feeding structure 8 can feed the material after the material is put in. The material is closed to prevent dust from leaking out. Under the action of the driving motor 3, the reduction box 4 drives the mixing structure 9 to move inside the silo 1 to complete the mixing of the powder. The mixing structure 9 combines the mixing technology of flipping and stirring, so that the material can move in multiple directions during the mixing process, increasing the contact between the materials to improve the uniformity of the mixing. At the same time, the side wall of the silo 1 can be scraped to improve the mixing efficiency of the powder. A switch solenoid valve is provided on the inner side of the discharge port 10, and three buckles 6 and fastening bolts 7 are provided. The inspection cover 5 can be opened or closed on the silo 1 by cooperating with the fastening bolts 7 and the buckle 6, which is convenient for cleaning and maintenance of the inside of the silo 1 and reduces the risk of cross contamination.

[0044] The feeding structure 8 includes a silo cover 81 fixed to the silo 1 on the left side, a driving assembly 82 arranged on the upper right side of the rear part of the silo cover 81, a lifting rod 83 connected to the top side of the front part of the driving assembly 82, and a cover plate 84 rotatably connected to the top side of the rear part of the lifting rod 83. Under the action of the driving assembly 82, the lifting rod 83 drives the cover plate 84 to flip, and the support rod 85 rotatably connected to the left part of the bottom side of the cover plate 84, the support seat 86 rotatably connected to the rear part of the lower middle side of the support rod 85, and the bottom end of the support rod 85 rotatably connected to the bottom end of the support rod 85. The material tray 87 and the cushion block 88 embedded in the upper middle side of the right side wall of the silo 1, the cover plate 84 is arranged on the upper right side of the silo cover 81, and the rear side of the support 86 is fixed to the silo cover 81. The cover plate 84 can be turned downward by the support of the support rod 85 to enter the silo cover 81 in an inclined state. At the same time, the material tray 87 is lifted upward and moved outward under the support of the cushion block 88 through the rotation of the support rod 85, so as to facilitate the placement of materials. The left part of the material tray 87 is provided with an upward tilt angle to shield the materials. In order to prevent the material from slipping out when placing, the driving assembly 82 includes a rectangular seat 821 fixed to the bin cover 81 at the front side, a servo motor 822 fastened to the lower right side of the rectangular seat 821, a worm 823 connected to the output end of the top of the servo motor 822, two support blocks 824 arranged on the upper and lower sides of the worm 823, a worm wheel 825 meshingly driven on the left side of the worm 823, a support frame 826 arranged on the front side of the worm wheel 825, and a rotating shaft column 827 fixed to the middle side of the front part of the worm wheel 825, and the support block 824 is provided. The right side of 24 is fixed to the rectangular seat 821, the left side of the support frame 826 is fixed to the rectangular seat 821, the shaft column 827 penetrates and rotates inside the support frame 826, and the front end of the shaft column 827 is fastened to the lifting rod 83. Under the action of the servo motor 822, the worm 823 drives the worm wheel 825 to rotate stably in the support frame 826, so that the shaft column 827 drives the lifting rod 83 to move when the worm wheel 825 rotates, so that the cover plate 84 is in an open or closed state on the bin cover 81.

[0045] The mixing structure 9 includes a rotating rod 91 connected to the reduction box 4 at the top end, a bearing block 92 arranged on the outer surface of the rotating rod 91, and a first stirring component 93 connected to the bottom end of the rotating rod 91. The reduction box 4 drives the rotating rod 91 to rotate inside the bearing block 92 through the action of the driving motor 3. An arc block 94 is fixed to the outer side of the bottom of the first stirring component 93, a cross 95 is fixedly connected to the inner side of the arc block 94, a second stirring component 96 connected to the bottom side of the arc block 94, and a positioning column 97 connected to the middle side of the top of the second stirring component 96. The rear side of the bearing block 92 is fixed to the silo 1, and the outer sides of the first stirring component 93, the arc block 94 and the second stirring component 96 are all in contact with the inner wall of the silo 1. The middle side of the bottom of the stirring assembly 96 is fixed to the silo 1, and the top side of the positioning column 97 is connected to the first stirring assembly 93, so that the first stirring assembly 93 is supported and positioned through the positioning column 97. The first stirring assembly 93 and the second stirring assembly 96 have the same structure and size. Four arc blocks 94 and crosses 95 are provided, and the upper and lower sides are respectively connected to the first stirring assembly 93 and the second stirring assembly 96, so that when the first stirring assembly 93 is in motion, the arc blocks 94 and the crosses 95 drive the second stirring assembly 96 to rotate. The first stirring assembly 93 includes a support tube 931, four rotating racks 932 respectively connected to the outer surface of the support tube 931 at four axial positions, and fixed to the outer side of the rotating rack 932. The scraper frame 933, three blades 934 that rotate through the inner side of the rotating frame 932, a rotating piece 935 fixedly connected to the side of the blade 934 close to the support tube 931, a convex ball rod 936 fixedly connected to the end of the rotating piece 935 away from the blade 934, and three guide grooves 937 arranged on the outer surface of the support tube 931 in a stepped manner. The top side of the rotating frame 932 is connected to the rotating rod 91. When the rotating rod 91 rotates, the rotating frame 932 and the scraper frame 933 are driven to rotate outside the support tube 931. It is fixed to the positioning column 97 at the middle side of the bottom of the support tube 931 to ensure the stability of the support tube 931. The outer side of the scraper frame 933 contacts the inner wall of the silo 1, and the bottom side of the scraper frame 933 is connected to the arc block 94 and the cross 9 5 are fixed to drive the arc block 94 and the cross 95 to move synchronously when the scraper frame 933 rotates. The spherical end of the convex ball rod 936 is inserted and slid inside the guide groove 937. During the rotation of the rotating frame 932, the convex ball rod 936 is driven to move in the guide groove 937 of the support tube 931. When the convex ball rod 936 moves to the lowest side position in the guide groove 937, the convex ball rod 936 drives the blade 934 to rotate to a vertical state. When the convex ball rod 936 moves to the highest side position in the guide groove 937, the convex ball rod 936 drives the blade 934 to rotate to a horizontal state, so that the blade 934 performs a flipping action of the rotation position, so that the material can move in multiple directions during the mixing process to improve the uniformity of the mixing.

[0046] The present invention provides an animal Bifidobacterium lactis subspecies B08 probiotic preparation with intestinal colonization function and a preparation method thereof. The working principle of the mixing equipment required to be used in the preparation process is as follows:

[0047] First, the present invention is placed at a position where the probiotic powder, microcrystalline cellulose and carrageenan need to be mixed through the silo 1 to use the present invention;

[0048] Second, control and start the servo motor 822. Under the action of the servo motor 822, the worm 823 drives the worm wheel 825 to rotate stably in the support frame 826, so that the shaft column 827 drives the lifting rod 83 to lift upward through the rotation of the worm wheel 825, so that the cover plate 84 tilts downward in the bin cover 81 to be in an open state. When the cover plate 84 tilts downward, the cover plate 84 rotates the support rod 85 to make the material tray 87 tilt upward and move outward under the support of the cushion block 88. Then the user places the probiotic powder, microcrystalline cellulose and carrageenan on the material tray 87, and controls the servo motor 822 to reset the cover plate 84. During the reset process of the cover plate 84, the material tray 87 moves back into the bin cover 81 and tilts downward, so that the probiotic powder, microcrystalline cellulose and carrageenan are poured into the bin tube 1.

[0049] Third, after the material enters the silo 1, the user starts the drive motor 3, and the drive motor 3 causes the rotating rod 91 to rotate inside the bearing block 92 through the reduction gear box 4. When the rotating rod 91 rotates, it drives the rotating frame 932 and the scraping frame 933 of the first stirring assembly 93 to rotate outside the support tube 931. When the scraping frame 933 of the first stirring assembly 93 rotates, it drives the arc block 94 and the cross 95 to move synchronously, so that the arc block 94 and the cross 95 move synchronously to drive the scraping frame 933 and the rotating frame 932 of the second stirring assembly 96 to rotate outside the support tube 931. During the rotation of the rack 932, the convex ball rod 936 is driven to move in the guide groove 937 of the support tube 931. When the convex ball rod 936 moves to the lowest side position in the guide groove 937, the convex ball rod 936 drives the blade 934 to rotate to a vertical state. When the convex ball rod 936 moves to the highest side position in the guide groove 937, the convex ball rod 936 drives the blade 934 to rotate to a horizontal state, so that the three blades 934 on the four rotating racks 932 of the first stirring assembly 93 and the second stirring assembly 96 perform a flipping action of the rotation position, so that the materials can move in multiple directions during the mixing process to improve the uniformity of the mixing;

[0050] Fourth, after the powders are evenly mixed, the switch solenoid valve in the discharge port 10 can be opened to discharge and collect the mixed materials.

[0051] The above description is only a preferred example of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A probiotic preparation of animal Bifidobacterium lactis subspecies B08 with intestinal colonization function, wherein the probiotic preparation of animal Bifidobacterium lactis subspecies B08 was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on July 22, 2022, and its deposit number is CGMCC NO: 25376, characterized in that: The Bifidobacterium lactis subspecies B08 probiotic preparation comprises the following raw materials in weight fractions: 1 part of Bifidobacterium lactis subspecies B08, 3 parts of milk powder, 0.5 parts of yeast extract, 1 part of lactose, 0.5 parts of trehalose, 2 parts of microcrystalline cellulose and 0.1 parts of stabilizer.

2. The probiotic preparation of Bifidobacterium lactis subspecies B08 for colonizing intestinal function according to claim 1, characterized in that: The stabilizer is selected from one or both of gelatin and carrageenan.

3. The probiotic preparation of Bifidobacterium lactis subspecies B08 for colonizing intestinal function according to claim 1, characterized in that: The total colony count per unit mass of the Bifidobacterium lactis subspecies B08 is 108-1010 CFU / g.

4. The method for preparing a probiotic preparation of Bifidobacterium lactis subspecies B08 for colonizing intestinal function according to any one of claims 1 to 3, characterized in that: The preparation method of the animal Bifidobacterium lactis subspecies B08 probiotic preparation comprises the following steps: S1, select Bifidobacterium lactis subsp. B08 strain, inoculate it in a liquid culture medium made of milk powder, yeast extract and lactose, and culture it at 37°C for 24-48 hours until it reaches the logarithmic growth phase; S2. Separate the bacteria in the liquid culture medium by centrifugation at 4000-6000 rpm for 10-15 minutes, and collect the precipitated bacteria; S3. Wash the bacterial precipitate 2-3 times with sterile saline to remove residual substances in the culture medium; S4. Resuspend the washed bacteria in PBS buffer and adjust the concentration to 108-1010 CFU / ml; S5, adding freeze-drying protective agent trehalose to the bacterial suspension, and then filling the bacterial suspension into freeze-drying bottles, and freeze-drying to prepare a powdered probiotic preparation; S6. The freeze-dried probiotic powder is mixed evenly with microcrystalline cellulose and a stabilizer through a mixing device to form a final preparation form, and then the preparation is aseptically packaged using microencapsulation technology to protect the probiotics from the effects of gastric acid and bile salts.

5. The method for preparing a probiotic preparation of Bifidobacterium lactis subspecies B08 for colonizing intestinal function according to claim 4, characterized in that: The mixing device comprises a silo (1), a support frame (2) fastened to the top side of the front of the silo (1), a driving motor (3) fastened to the front side of the support frame (2), a reduction gear box (4) connected to the top output end of the driving motor (3), an inspection cover (5) hinged to the middle side of the front of the silo (1), a buckle (6) arranged on the left side of the front of the inspection cover (5), a fastening bolt (7) inserted and locked inside the buckle (6), a feeding structure (8) fixed to the upper right side of the silo (1), a mixing structure (9) connected to the rear side of the bottom of the reduction gear box (4), and a discharge port (10) arranged on the bottom side of the left side of the silo (1), wherein the reduction gear box (4) is installed on the top side of the silo (1), the rear side of the fastening bolt (7) is rotatably connected to the silo (1), the mixing structure (9) is arranged on the middle side of the inside of the silo (1), and a switch is arranged on the inside of the discharge port (10). The electromagnetic valve, the buckle (6) and the fastening bolt (7) are both provided in three numbers, the feeding structure (8) comprises a silo cover (81) fixed to the silo barrel (1) on the left side, a driving assembly (82) arranged on the upper right side of the rear part of the silo cover (81), a lifting rod (83) connected to the top side of the front part of the driving assembly (82), a cover plate (84) rotatably connected to the top side of the rear part of the lifting rod (83), a support rod (85) rotatably connected to the left part of the bottom side of the cover plate (84), a support seat (86) rotatably connected to the rear part of the middle and lower side of the support rod (85), a material tray (87) rotatably connected to the bottom end of the support rod (85), and a cushion block (88) embedded in the upper middle side of the right side wall of the silo barrel (1), the cover plate (84) is arranged on the upper right side of the silo cover (81), the rear side of the support seat (86) is fixed to the silo cover (81), and the left part of the material tray (87) is provided with an upward inclination angle.

6. The method for preparing a probiotic preparation of Bifidobacterium lactis subspecies B08 for colonizing intestinal function according to claim 5, characterized in that: The driving assembly (82) comprises a rectangular seat (821) fixed to the bin cover (81) at the front side, a servo motor (822) fastened to the lower right side of the rectangular seat (821), a worm (823) connected to the top output end of the servo motor (822), two support blocks (824) arranged at the upper and lower sides of the worm (823), a worm wheel (825) meshingly driven on the left side of the worm (823), a support frame (826) arranged at the front side of the worm wheel (825), and a rotating shaft column (827) fixed to the middle side of the front part of the worm wheel (825), the right side of the support block (824) is fixed to the rectangular seat (821), the left side of the support frame (826) is fixed to the rectangular seat (821), the rotating shaft column (827) penetrates and rotates on the inner side of the support frame (826), and the front end of the rotating shaft column (827) is fastened to the lifting rod (83).

7. The method for preparing a probiotic preparation of Bifidobacterium lactis subspecies B08 for colonizing intestinal function according to claim 5, characterized in that: The mixing structure (9) comprises a rotating rod (91) whose top end is connected to a reduction box (4), a bearing block (92) arranged on the outer surface of the rotating rod (91), a first stirring component (93) connected to the bottom end of the rotating rod (91), an arc block (94) fixed to the outside of the bottom of the first stirring component (93), a cross (95) fixedly connected to the inside of the arc block (94), a second stirring component (96) connected to the bottom side of the arc block (94), and a bearing block (92) connected to the second stirring component (96). A positioning column (97) is provided at the middle of the top, the rear side of the bearing block (92) is fixed to the silo (1), the outer sides of the first stirring component (93), the arc block (94) and the second stirring component (96) are all in contact with the inner wall of the silo (1), the middle side of the bottom of the second stirring component (96) is fixed to the silo (1), the top side of the positioning column (97) is connected to the first stirring component (93), and the first stirring component (93) and the second stirring component (96) have the same structure and size.

8. The method for preparing a probiotic preparation of Bifidobacterium lactis subspecies B08 for colonizing intestinal function according to claim 7, characterized in that: Four of the arc-shaped blocks (94) and the crosses (95) are provided, and the upper and lower sides thereof are respectively connected to the first stirring component (93) and the second stirring component (96).

9. The method for preparing a probiotic preparation of Bifidobacterium lactis subspecies B08 for colonizing intestinal function according to claim 7, characterized in that: The first stirring assembly (93) comprises a support tube (931), four rotating racks (932) respectively rotatably connected to the outer surface of the support tube (931) at four axial positions, a scraping rack (933) fixed to the outside of the rotating rack (932), three blades (934) penetrating and rotatably passing through the inside of the rotating rack (932), a rotating piece (935) fixedly connected to a side of the blades (934) close to the support tube (931), and a rotating piece (935) fixedly connected to the rotating piece (935) away from the blades (934). A convex ball rod (936) at one end of the joint and three guide grooves (937) arranged in a stepped manner on the outer surface of the support tube (931) are connected. The middle side of the bottom of the support tube (931) is fixed to the positioning column (97). The outer side of the scraper frame (933) contacts the inner wall of the silo (1), and the bottom side of the scraper frame (933) is fixed to the arc block (94) and the cross (95). The spherical end of the convex ball rod (936) is inserted and slidable inside the guide groove (937).

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