Rabies virus vaccine Vero cell DNA separation and extraction method

Abstract

The invention relates to the technical field of detection of DNA proportions of various components of rabies virus vaccine cells, in particular to a rabies virus vaccine Vero cell DNA separation and extraction method which comprises the following steps: cell lysis, combination, separation, washing and dissociation. The traditional test tube horizontal rotary oscillation is adjusted into test tube inclined rotary swing oscillation, so that the magnetic beads at the bottom of the test tube are floated along with oscillation and then are adsorbed by the glass rod sleeve, the Vero cell DNA is adsorbed, extracted and purified as much as possible, the detection precision of the Vero cell DNA residue is improved, and the deviation of the detection result is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of DNA ratio detection of various components of rabies virus vaccine cells, in particular to a method for separating and extracting DNA of Vero cells of rabies virus vaccine. Background Art

[0002] Rabies is an acute zoonotic infectious disease caused by a virus of the genus Lyssavirus. It usually leads to acute encephalitis or meningitis, with a mortality rate of almost 100%. Post-exposure management mainly includes the use of rabies vaccine and rabies immunoglobulin, which is the only effective means to prevent rabies after exposure.

[0003] At present, the cell matrices used in the production of human rabies vaccines that have been marketed in China mainly include primary cell lines (PCLs), continuous cell lines (CCLs) and diploid cell lines (DCLs). The representative cell matrices are primary hamster kidney cells, Vero cells and MRC-5 cells, among which Vero cell bioreactor fermentation production is the main method. However, the residual DNA of Vero cells has safety risks such as tumorigenicity, transmission of exogenous factors and teratogenicity. In order to ensure product quality and ensure the safety of patients' medication, it is necessary to confirm the amount of residual DNA in the production process.

[0004] The third volume of the Chinese Pharmacopoeia includes the detection of residual Vero cell DNA by RT-PCR technology. The fluorescence method can detect double-stranded DNA from human and expression host samples. It is convenient and fast, but it is easily interfered by RNA, ssDNA, and dsDNA, and is easily affected by standard products. This influence mainly refers to the degradation and inactivation of standard products due to improper handling during transportation and storage.

[0005] The Chinese patent application number CN202311019339.1 specifically discloses a method for detecting rabies vaccine DNA residues using digital PCR, including the following steps: extracting DNA from human rabies vaccine stock samples; establishing a digital PCR system; preparing droplets; performing digital PCR amplification; and calculating the concentration of the residual copy number of Vero cell DNA in human rabies vaccine samples obtained by digital PCR amplification as a national standard unit value. Primers and probes were designed based on the GP gene sequence of rabies virus, and the reaction conditions and reaction procedures were optimized. The establishment of a digital PCR method was evaluated through specificity and repeatability tests, thereby establishing a method for detecting human rabies vaccine DNA residues using digital PCR.

[0006] However, when the above-mentioned detection method is used to pre-purify the DNA of rabies vaccine Vero cells, since the bottom of the test tube is hemispherical, when the DNA is extracted by the magnetic bead method, the magnetic beads will be deposited on the hemispherical bottom of the test tube, and the glass rod sleeve that absorbs the magnetic beads cannot extend to the bottom. At the same time, the horizontal oscillation mode of the test tube also causes the magnetic beads deposited at the bottom of the test tube to not be well shaken and floated, so that the DNA extraction cannot reach the preset extraction level, which in turn causes deviations in the test results. Summary of the invention

[0007] In view of the above problems, the present invention provides a method for separating and extracting Vero cell DNA of rabies virus vaccine. When adsorbing and extracting magnetic beads, the oscillation mode of the test tube is changed from the traditional horizontal cyclonic oscillation of the test tube to the tilted rotation swinging oscillation of the test tube, so that the magnetic beads at the bottom of the test tube are floated with the oscillation and then adsorbed by the glass rod sleeve, so that as much Vero cell DNA as possible can be adsorbed, extracted and purified, thereby improving the accuracy of Vero cell DNA residue detection and avoiding deviations in detection results.

[0008] To achieve the above object, the present invention provides the following technical solutions:

[0009] A method for separating and extracting rabies virus vaccine Vero cell DNA comprises the following steps:

[0010] Step s1, cell lysis, using a mixture containing a lysis buffer to break the Vero cells and release the nucleic acids and proteins therein;

[0011] Step s2, combining, mixing the lysed sample with the magnetic beads and loading them into a test tube, vertically inserting the test tube into an oscillating device, inserting a glass rod sleeve into the test tube, and when the oscillating device drives the test tube to oscillate, the magnetic beads are evenly distributed in the test tube through the stirring of the glass rod sleeve, and the magnetic beads are combined with the DNA through the DNA-affinity molecules coated on the surface;

[0012] Step s3, separation, inserting a magnetic rod into the glass rod sleeve, the oscillation device drives the test tube to swing, so that the test tube is inclined, and the central axis thereof is swung around the circumference of the glass rod sleeve, the magnetic rod absorbs the magnetic beads, and the DNA separation process is completed;

[0013] Step s4, washing, adding washing buffer to the magnetic beads to remove non-specific binding or residual impurities, and repeating the washing step using magnetic force, removing the washing solution after each washing;

[0014] Step s5, detachment: After the DNA is fully washed, an ion solution or a low-salt buffer is added to make the DNA fall off the magnetic beads and enter the solution.

[0015] As an improvement, in step s1, the mixture containing the lysis buffer includes a buffer, a non-ionic detergent, a salt, a protease inhibitor, a nuclease inhibitor, a stabilizer, an antioxidant and an energy substance.

[0016] As an improvement, in step s2, the DNA-affinity molecules coated on the surface of the magnetic beads include one or more of oligodeoxynucleotides, streptavidin, protein A, protein G or histones.

[0017] As an improvement, in step s2 and step s3, the test tube includes a straight tube portion and a hemispherical bottom portion at the bottom of the tube portion, and the lower end portion of the glass rod sleeve is located at the connection position between the tube portion and the bottom portion.

[0018] As an improvement, in step s2 and step s3, the oscillating device includes a test tube rack, a test tube ring, a lifting component and a driving component;

[0019] The test tube rack is provided with a plurality of insertion holes for inserting test tubes, and insertion tubes are correspondingly arranged below the insertion holes;

[0020] The test tube ring is arranged at the insertion hole, the test tube ring is slidably arranged relative to the insertion hole, and the test tube ring is arranged around the tube portion of the test tube, and initially, the bottom of the test tube ring is arranged to abut against the top of the insertion tube;

[0021] The lifting assembly is installed at the lower part of the test tube collar, and the lifting assembly lifts the test tube collar;

[0022] The driving component is arranged below the test tube ring, and the driving component drives the test tube to swing.

[0023] As an improvement, the test tube ring includes an elastic ring and an air bag;

[0024] The elastic ring is located in the inner ring of the test tube ring, and the elastic ring is elastically expandable and contractible;

[0025] The air bag is arranged on the outer ring of the test tube sleeve, and the air bag is inserted and matched with the insertion hole.

[0026] As an improvement, the lifting assembly includes a lifting plate and an electric push cylinder;

[0027] The lifting plate is arranged horizontally, and is lifted and lowered in a vertical direction by an electric push cylinder arranged vertically below the lifting plate.

[0028] As an improvement, the driving assembly includes a driving bevel ring, a transmission gear, a driving gear, a driving rack and a driving motor;

[0029] The driving oblique ring is rotatably mounted on the lifting plate, the driving oblique ring is coaxially arranged with the test tube ring, and the upper end surface of the driving oblique ring is arranged as an inclined surface;

[0030] The transmission gear is sleeved on the driving oblique ring, and along the setting direction of the driving rack, the transmission gears on the adjacent driving oblique rings are meshed with each other;

[0031] The driving gear is coaxially arranged with the transmission gear, and the driving gear is located above the transmission gear;

[0032] The driving rack is horizontally arranged, and the driving rack is telescopically moved horizontally, meshing with the adjacent driving gear, and driving the driving oblique ring to rotate;

[0033] The driving motor is connected to the driving rack through a connecting rod group, and the driving motor drives the driving rack to move telescopically.

[0034] As an improvement, in step s2 and step s3, a sealing airbag is provided on the top of the glass rod sleeve, and after the glass rod sleeve is inserted into the test tube, the sealing airbag is inflated to seal the top opening of the test tube.

[0035] As an improvement, in step s4, the components of the washing buffer include one or more of ethanol or isopropanol.

[0036] The beneficial effects of the present invention are:

[0037] (1) When the magnetic beads are adsorbed and extracted, the present invention changes the oscillation mode of the test tube from the traditional horizontal cyclotron oscillation of the test tube to the tilted rotation swing oscillation of the test tube, so that the magnetic beads at the bottom of the test tube are floated with the oscillation and then adsorbed by the glass rod sleeve, so that as much Vero cell DNA as possible is adsorbed, extracted and purified, thereby improving the accuracy of Vero cell DNA residue detection and avoiding deviation in the detection results;

[0038] (2) When the test tube is driven to oscillate by the oscillation device of the present invention, a sealing airbag is arranged at the top opening of the test tube, and the top of the test tube is sealed by inflating the sealing airbag, so as to prevent the liquid inside from splashing out due to the tilted setting of the test tube when the test tube is oscillated, thereby ensuring the stability of the oscillation;

[0039] (3) When the test tube is driven to oscillate by the oscillation device of the present invention, the test tube is always in an inclined setting, so that during the oscillation process, the test tube oscillates more violently than the original vertical and horizontal oscillations, and the liquid inside the test tube oscillates more violently. In addition, since the test tube is tilted and unbalanced on the left and right, the magnetic beads can be dispersed during the oscillation, and the magnetic beads settled at the bottom of the test tube can be better floated. In addition, the distance from the bottom of the tilted test tube to the bottom of the glass rod sleeve is shortened, which is more conducive to the glass rod sleeve adsorbing the magnetic beads and completing the adsorption and extraction of DNA.

[0040] In summary, the present invention has the advantages of high degree of Vero cell DNA adsorption and extraction, high detection accuracy, etc., and is particularly suitable for the technical field of Vero cell DNA residue detection for rabies virus. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a schematic diagram of the three-dimensional structure of the DNA separation and extraction system of the present invention;

[0042] Figure 2 It is a schematic diagram of the three-dimensional structure of the test tube of the present invention;

[0043] Figure 3 This is a schematic diagram of the matching structure of the test tube and the glass rod sleeve of the present invention;

[0044] Figure 4 This is a schematic diagram of the test tube oscillation state of the present invention;

[0045] Figure 5 It is a schematic diagram of the three-dimensional structure of the oscillation device of the present invention;

[0046] Figure 6 This is a schematic diagram of the three-dimensional structure of the test tube rack of the present invention;

[0047] Figure 7 The sectional structure diagram of the oscillating device of the present invention is shown in FIG. Figure 1 ;

[0048] Figure 8 The sectional structure diagram of the oscillating device of the present invention is shown in FIG. Figure 2 ;

[0049] Fig. 9 A schematic diagram of the partial structure of the oscillating device of the present invention;

[0050] Fig.10 It is a schematic diagram of a partial cross-sectional structure of a test tube ring of the present invention;

[0051] Fig.11 It is a schematic diagram of the three-dimensional structure of the lifting plate of the present invention;

[0052] Fig.12 This is a schematic diagram of the driving oblique ring cross-section structure of the present invention;

[0053] Fig.13 It is a schematic diagram of the three-dimensional structure of the sealing airbag of the present invention.

[0054] In the figure: 1. test tube, 11. tube part, 12. bottom, 2. oscillating device, 21. test tube rack, 211. socket, 212. cannula, 22. test tube ring, 221. elastic ring, 222. air bag, 23. lifting assembly, 231. lifting plate, 232. electric push cylinder, 24. driving assembly, 241. driving oblique ring, 242. transmission gear, 243. driving gear, 244 driving rack, 245. driving motor, 246. connecting rod group, 3. glass rod sleeve, 31. sealing air bag, 32. pipeline, 33. air supply joint, 4. magnetic rod. DETAILED DESCRIPTION

[0055] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0056] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0057] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0058] Embodiment 1:

[0059] A method for separating and extracting rabies virus vaccine Vero cell DNA, characterized in that it comprises the following steps:

[0060] Step s1, cell lysis, using a mixture containing a lysis buffer to break the Vero cells and release the nucleic acids and proteins therein;

[0061] Step s2, combining, mixing the lysed sample with the magnetic beads and loading them into a test tube 1, vertically inserting the test tube 1 on an oscillating device 2, inserting a glass rod sleeve 3 into the test tube 1, and when the oscillating device 2 drives the test tube 1 to oscillate, the magnetic beads are evenly distributed in the test tube 1 through the stirring of the glass rod sleeve 3, and the magnetic beads are combined with DNA through the DNA-affinity molecules coated on the surface;

[0062] Step s3, separation, inserting the magnetic rod 4 into the glass rod sleeve 3, the oscillating device 2 drives the test tube 1 to swing, so that the test tube 1 is inclined, and its central axis is set to swing around the glass rod sleeve 3, the magnetic rod 4 absorbs the magnetic beads, and the DNA separation process is completed;

[0063] Step s4, washing, adding washing buffer to the magnetic beads to remove non-specific binding or residual impurities, and repeating the washing step using magnetic force, removing the washing solution after each washing;

[0064] Step s5, detachment: After the DNA is fully washed, an ion solution or a low-salt buffer is added to make the DNA fall off the magnetic beads and enter the solution.

[0065] Wherein, in step s1, the mixture containing the lysis buffer includes a buffer, a non-ionic detergent, a salt, a protease inhibitor, a nuclease inhibitor, a stabilizer, an antioxidant and an energy substance.

[0066] Furthermore, in step s2, the DNA-affinity molecules coated on the surface of the magnetic beads include one or more of oligodeoxynucleotides, streptavidin, protein A, protein G or histones.

[0067] Furthermore, in step s4, the components of the washing buffer include one or more of ethanol or isopropanol.

[0068] It is further explained that, when the Vero cell DNA is adsorbed, extracted and purified, the test tube carrying the Vero cell DNA is placed at an angle so that the glass rod sleeve that adsorbs the magnetic beads can be closer to the bottom of the test tube, and the test tube is shaken to facilitate the glass rod sleeve to capture the magnetic beads through the magnetic rod. The magnetic beads float up with the shaking with greater intensity, and the magnetic beads settled at the bottom of the test tube are more easily floated up and captured.

[0069] Embodiment 2:

[0070] In combination with the first embodiment, a system for separating and extracting DNA from Vero cells according to the second embodiment of the present invention is described.

[0071] like Figures 1 to 12As shown, specifically, the test tube 1 includes a straight tube portion 11 and a hemispherical bottom portion 12 at the bottom of the tube portion 11 , and the lower end portion of the glass rod sleeve 3 is located at the connection position between the tube portion 11 and the bottom portion 12 .

[0072] The oscillating device 2 includes a test tube rack 21, a test tube ring 22, a lifting component 23 and a driving component 24;

[0073] The test tube rack 21 is provided with a plurality of insertion holes 211 for inserting the test tubes 1, and the insertion tubes 212 are correspondingly arranged below the insertion holes 211. When inserting the test tubes, the lower part of the test tubes 1 is inserted into the insertion tubes 212, and the upper part of the test tubes 1 is in the insertion holes 211. The diameters of the insertion holes 211 and the insertion tubes 212 are larger than the diameter of the test tubes 1, so that the test tubes 212 can be placed obliquely in the insertion holes 211 and the insertion tubes 212.

[0074] The test tube ring 22 is arranged at the insertion hole 211, and the test tube ring 22 is slidably arranged relative to the insertion hole 211, and the test tube ring 22 is arranged around the tube portion 11 of the test tube 1, and initially, the bottom of the test tube ring 22 is arranged to abut against the top of the insertion tube 212, and the test tube 1 is placed in a vertical state through the horizontal support of the top of the insertion tube 212, which is convenient for the subsequent insertion of the glass rod sleeve 3;

[0075] The test tube ring 22 includes an elastic ring 221 and an air bag 222; the elastic ring 221 is located in the inner ring of the test tube ring 22, and the elastic ring 221 is elastically expandable and contractible; the air bag 222 is arranged in the outer ring of the test tube ring 22, and the air bag 222 is arranged to be interlaced and matched with the insertion hole 211. The diameter of the elastic ring 221 is smaller than the diameter of the test tube 1. After the test tube 1 is inserted into the elastic ring 221, the elastic ring 221 will expand and hold the test tube 1 tightly, while the air bag 222 keeps the test tube 1 always located at the center of the insertion hole 211, and the setting structure of the air bag 222 makes the test tube 1 both in a tightly held state and can be tilted and adjusted;

[0076] The lifting assembly 23 is installed under the test tube ring 22, and the lifting assembly 23 lifts the test tube ring 22. The lifting assembly 23 includes a lifting plate 231 and an electric push cylinder 232; the lifting plate 231 is horizontally arranged, and the lifting plate 231 is lifted and lowered in the vertical direction by the electric push cylinder 232 vertically arranged below it;

[0077] The driving assembly 24 is arranged below the test tube ring 22, and the driving assembly 24 drives the test tube 1 to swing. The driving assembly 24 includes a driving oblique ring 241, a transmission gear 242, a driving gear 243, a driving rack 244 and a driving motor 245; the driving oblique ring 241 is rotatably mounted on the lifting plate 231, and the driving oblique ring 241 is coaxially arranged with the test tube ring 22, and the upper end surface of the driving oblique ring 241 is an inclined surface. When the lifting assembly 23 lifts the lifting plate 231, the driving oblique ring 241 The test tube 1 is also lifted up, and the upper end surface of the driving oblique ring 241 contacts with the lower end surface of the test tube ring 22, so that the test tube 1 is lifted up and separated from the support of the top of the cannula 212. Due to the flexible setting of the airbag 222 on the test tube ring 22 and the inclined setting of the upper end surface of the driving oblique ring 241, the test tube 1 is tilted and supported on the upper end surface of the driving oblique ring 241 in cooperation with the gravity of the test tube 1 itself, and at this time, the bottom of the test tube 1 just contacts with the inner side wall of the cannula 212. When the test tube 1 oscillates and swings, the bottom of the test tube 1 is just supported by the inner side wall of the cannula 212.

[0078] The transmission gear 242 is sleeved on the driving oblique ring 241, and along the setting direction of the driving rack 244, the transmission gears 242 on adjacent driving oblique rings 241 are meshed with each other; the driving gear 243 is coaxially arranged with the transmission gear 242, and the driving gear 243 is located above the transmission gear 242, and the driving gear 243 is only arranged above the transmission gear 242 close to the driving rack 244; the driving rack 244 is horizontally arranged, and the driving rack 244 is horizontally telescopically moved, meshing with the adjacent driving gear 243, and driving the driving oblique ring 241 to rotate; the driving motor 245 is connected to the driving rack 244 through a connecting rod group 246, and the driving motor 245 drives the driving rack 244 to telescopically move Specifically, after the lifting plate 231 is lifted, the driving gear 243 meshes with the driving rack 242, and the connecting rod group 246 transmits power to the driving rack 244 through the drive of the driving motor 245. The driving rack 244 pushes back and forth horizontally, driving the driving gear 243 to rotate, so that the corresponding driving oblique ring 241 rotates. During the rotation of the driving oblique ring 241, the supporting point A of the test tube 1 supported by the driving oblique ring 241 changes continuously, so that the central axis of the test tube 1 swings around the glass rod sleeve 3, so that the magnetic beads settled at the bottom of the test tube 1 are constantly agitated and floated, and are absorbed by the glass rod sleeve 3, and are transmitted through the meshing of the transmission gear 242, so that all the driving oblique rings 241 rotate and swing synchronously, driving all the test tubes 1 to rotate and oscillate synchronously.

[0079] The magnetic bar 4 in the present invention is preferably a rare earth magnet such as sintered neodymium iron boron (NdFeB), which can provide a strong magnetic field to quickly and reliably separate samples with magnetic beads.

[0080] In addition, it should be described that the lifting structures of the glass rod sleeve 3 and the magnetic rod 4 are both independently arranged screw lifting mechanisms. The screw lifting mechanism is a prior art and will not be described in detail. The glass rod sleeve 3 is located below the magnetic rod 4. The magnetic rod 4 can be inserted into the glass rod sleeve 3, so that the surface of the glass rod sleeve 3 forms a magnetic force that adsorbs the magnetic beads. After the magnetic rod 4 is separated from the glass rod sleeve 3, the magnetic beads can be separated from the adsorption of the glass rod sleeve 3 by washing.

[0081] Embodiment 3:

[0082] The distinguishing technical features of Example 3 of the present invention are described with reference to Example 2:

[0083] like Figure 3 , Figure 4 and Fig.13 As shown, the top of the glass rod sleeve 3 is provided with a sealing air bag 31 . After the glass rod sleeve 3 is inserted into the test tube 1 , the sealing air bag 31 is inflated to seal the top opening of the test tube 1 .

[0084] It should be noted that in order to ensure that the liquid in the test tube 1 does not leak when the test tube 1 is oscillating, after the glass rod sleeve 3 is inserted into the test tube 1, the sealing airbag 31 moves with the glass rod sleeve 3 to the opening at the top of the test tube 1, and then gas is filled into the sealing airbag 31 through an external inflation device, so that the sealing airbag 31 expands, thereby forming a seal on the opening of the test tube 1, and the sealing airbag 31 is flexibly arranged, so that even if the test tube 1 is tilted later, the sealing airbag 31 can still form a good seal on the opening of the test tube 1.

[0085] In addition, different sealing airbags 31 are connected to each other through a pipe 32 , and the pipe 32 is connected to an external air supply device through an air supply connector 33 .

[0086] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for separating and extracting rabies virus vaccine Vero cell DNA, characterized in that: The following steps are involved: Step s1, cell lysis, using a mixture containing a lysis buffer to break the Vero cells and release the nucleic acids and proteins therein; Step s2, combining, mixing the lysed sample with the magnetic beads and loading them into a test tube (1), vertically inserting the test tube (1) on an oscillating device (2), inserting a glass rod sleeve (3) into the test tube (1), and when the oscillating device (2) drives the test tube (1) to oscillate, the magnetic beads are evenly distributed in the test tube (1) through the stirring of the glass rod sleeve (3), and the magnetic beads are combined with DNA through the DNA-affinity molecules coated on the surface; Step s3, separation, inserting a magnetic rod (4) into the glass rod sleeve (3), the oscillating device (2) drives the test tube (1) to swing, so that the test tube (1) is tilted, and the central axis thereof is swung in a circle around the glass rod sleeve (3), the magnetic rod (4) adsorbs the magnetic beads, and the DNA separation process is completed; Step s4, washing, adding washing buffer to the magnetic beads to remove non-specific binding or residual impurities, and repeating the washing step using magnetic force, removing the washing solution after each washing; Step s5, detachment: After the DNA is fully washed, an ion solution or a low-salt buffer is added to make the DNA fall off the magnetic beads and enter the solution.

2. A method for detecting residual DNA of Vero cells of rabies virus vaccine according to claim 1, characterized in that: In step s1, the mixture containing the lysis buffer includes a buffer, a non-ionic detergent, a salt, a protease inhibitor, a nuclease inhibitor, a stabilizer, an antioxidant and an energy substance.

3. A method for detecting residual DNA of Vero cells of rabies virus vaccine according to claim 1, characterized in that: In step s2, the DNA-affinity molecules coated on the surface of the magnetic beads include one or more of oligodeoxynucleotides, streptavidin, protein A, protein G or histones.

4. A method for detecting residual DNA of Vero cells of rabies virus vaccine according to claim 1, characterized in that: In the steps s2 and s3, the test tube (1) comprises a straight tube portion (11) and a hemispherical bottom portion (12) located at the bottom of the tube portion (11), and the lower end portion of the glass rod sleeve (3) is located at the connection position between the tube portion (11) and the bottom portion (12).

5. A method for detecting residual Vero cell DNA of rabies virus vaccine according to claim 4, characterized in that: In the step s2 and the step s3, the oscillating device (2) comprises a test tube rack (21), a test tube ring (22), a lifting component (23) and a driving component (24); The test tube rack (21) is provided with a plurality of insertion holes (211) for inserting the test tubes (1), and insertion tubes (212) are correspondingly arranged below the insertion holes (211); The test tube ring (22) is arranged at the insertion hole (211), the test tube ring (22) is slidably arranged relative to the insertion hole (211), and the test tube ring (22) is arranged around the tube portion (11) of the test tube (1) to hold it tightly. Initially, the bottom of the test tube ring (22) is arranged to abut against the top of the insertion tube (212); The lifting component (23) is installed at the bottom of the test tube ring (22), and the lifting component (23) lifts the test tube ring (22) to set; The driving component (24) is arranged below the test tube ring (22), and the driving component (24) drives the test tube (1) to swing.

6. A method for detecting residual Vero cell DNA of rabies virus vaccine according to claim 5, characterized in that: The test tube ring (22) comprises an elastic ring (221) and an air bag (222); The elastic ring (221) is located in the inner ring of the test tube ring (22), and the elastic ring (221) is elastically expandable and contractible; The air bag (222) is arranged on the outer ring of the test tube ring (22), and the air bag (222) and the insertion hole (211) are arranged in an interlaced and matched manner; 7. The method for detecting residual Vero cell DNA of rabies virus vaccine according to claim 5, characterized in that: The lifting assembly (23) comprises a lifting plate (231) and an electric push cylinder (232); The lifting plate (231) is arranged horizontally, and the lifting plate (231) is lifted and lowered in the vertical direction by an electric push cylinder (232) arranged vertically below the lifting plate (231).

8. The method for detecting residual Vero cell DNA of rabies virus vaccine according to claim 7, characterized in that: The driving assembly (24) comprises a driving oblique ring (241), a transmission gear (242), a driving gear (243), a driving rack (244) and a driving motor (245); The driving oblique ring (241) is rotatably mounted on the lifting plate (231), the driving oblique ring (241) is coaxially arranged with the test tube ring (22), and the upper end surface of the driving oblique ring (241) is arranged as an inclined surface; The transmission gear (242) is sleeved on the driving oblique ring (241), and along the setting direction of the driving rack (244), the transmission gears (242) on adjacent driving oblique rings (241) are meshed with each other; The driving gear (243) is coaxially arranged with the transmission gear (242), and the driving gear (243) is located above the transmission gear (242); The driving rack (244) is arranged horizontally, and the driving rack (244) moves horizontally in telescopic manner, meshes with the adjacent driving gear (243), and drives the driving oblique ring (241) to rotate; The driving motor (245) is connected to the driving rack (244) via a connecting rod group (246), and the driving motor (245) drives the driving rack (244) to move telescopically.

9. The method for detecting residual DNA of Vero cells of rabies virus vaccine according to claim 1, characterized in that: In the steps s2 and s3, the top of the glass rod sleeve (3) is provided with a sealing airbag (31). After the glass rod sleeve (3) is inserted into the test tube (1), the sealing airbag (31) is inflated to seal the top opening of the test tube (1).

10. The method for detecting residual DNA of Vero cells of rabies virus vaccine according to claim 1, characterized in that: In step s4, the components of the washing buffer include one or more of ethanol or isopropanol.

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