Acne-removing compositions that prevent acne scars and their applications, cosmetics

Abstract

This application relates to the field of cosmetics, specifically an acne-reducing composition for blocking acne scars and its application in cosmetics. The composition comprises, by weight, 0.5-2.5 parts maltodextrin, 0.2-1.5 parts arginine, and 1-10 parts peony root extract. Maltodextrin can reduce inflammatory responses, decrease inflammatory cell infiltration around hair follicles, and inhibit the release of inflammatory mediators such as interleukins. Arginine can improve skin blood circulation; acne is partly caused by blockage of the hair follicle sebaceous gland duct, leading to poor blood circulation. The NO produced by arginine can dilate blood vessels and increase blood perfusion to the skin. This helps deliver nutrients and immune cells to the site of skin inflammation. Peony root extract can regulate inflammation-related signaling pathways and reduce the production of inflammatory factors; thus, it can further synergistically enhance the effects of arginine and maltodextrin to reduce acne scars.

Description

Technical Field

[0001] This application relates to the field of cosmetics, and more specifically, to an acne-removing composition for preventing acne scars, its application, and cosmetics. Background Technology

[0002] As people pay more attention to skin health and appearance, the market for acne and acne scar removal products continues to expand, and the technology has gone through several stages of development.

[0003] Early products often used natural herbs, such as tea tree oil, to alleviate mild acne due to its anti-inflammatory and antibacterial properties, but were less effective for moderate to severe acne. Later, chemically synthesized ingredients became mainstream, such as salicylic acid and fruit acids, which can regulate keratin metabolism and unclog pores, but are highly irritating, especially to sensitive skin, limiting their application. Regarding acne scar removal, current methods typically rely on whitening ingredients such as vitamin C and its derivatives to inhibit melanin synthesis. However, these have limited effectiveness on deeper acne scars. Therefore, existing products suffer from the problem of irritating sensitive skin and failing to provide a comprehensive, effective, and gentle solution for acne and acne scar removal. Summary of the Invention

[0004] The purpose of this application is to provide an acne-removing composition for blocking acne scars, its application, and cosmetics.

[0005] In a first aspect, this application provides an acne-removing composition for blocking acne scars, wherein, by weight parts, the acne-removing composition for blocking acne scars comprises:

[0006] Maltodextrin 0.5-2.5 parts, arginine 0.2-1.5 parts and peony root extract 1-10 parts.

[0007] In the above technical solution, maltodextrin, arginine, and peony root extract are combined to achieve a synergistic effect, effectively improving the treatment of acne and acne scars. The three ingredients work synergistically to reduce the transmission of TNF-α inflammatory factors, minimize the impact on blood vessels and melanocytes, inhibit acne worsening, and prevent acne scar formation, thus greatly enhancing the treatment of acne and acne scars. Applying this composition to cosmetics helps solve the problems of existing products irritating sensitive skin and failing to provide comprehensive, effective, and gentle treatment for acne and acne scars.

[0008] Furthermore, maltodextrin in the above composition can alleviate inflammatory responses by reducing inflammatory cell infiltration around hair follicles and inhibiting the release of inflammatory mediators such as interleukins (IL-1β, IL-6, etc.). Arginine can improve skin blood circulation; and acne is partly caused by blockage of the hair follicle sebaceous gland duct, leading to poor blood circulation. NO produced by arginine can dilate blood vessels and increase blood perfusion to the skin. This helps deliver nutrients and immune cells to the site of skin inflammation. Combining arginine with maltodextrin can achieve a synergistic effect, accelerating the reduction of TNF-α inflammatory factor transmission while significantly reducing acne scars. Peony root extract can regulate inflammation-related signaling pathways and reduce the production of inflammatory factors; thus, it can further synergize with arginine and maltodextrin, enhancing the effects of acne treatment and acne scar removal from different targets.

[0009] In other embodiments of this application, the acne-removing composition for blocking acne scars, by weight, comprises:

[0010] Maltodextrin 1.0-2.0 parts, arginine 0.40-1.0 parts and peony root extract 5-8 parts.

[0011] In other embodiments of this application, in the acne-removing composition for blocking acne scars, the mass ratio of maltodextrin to arginine is (2~5):1.

[0012] In other embodiments of this application, in the acne-removing composition for blocking acne scars, the mass ratio of maltodextrin to arginine is (2.5~4):1.

[0013] In other embodiments of this application, the pH value of the acne-removing composition for blocking acne scars is 3.0-5.5.

[0014] In other embodiments of this application, the pH value of the acne-removing composition for blocking acne scars is 4.0-5.5.

[0015] In other embodiments of this application, the optical rotation of arginine ranges from 26.0° to 27.9°.

[0016] In other embodiments of this application, peony root extract is added to the composition in solution form.

[0017] In other embodiments of this application, the peony root extract solution contains ≥8% peony root extract by mass percentage.

[0018] In other embodiments of this application, the paeoniflorin active ingredient in the peony root extract is ≥1% by mass percentage.

[0019] In other embodiments of this application, the peony root extract solution contains peony root extract and a solvent; optionally, the solvent includes butanediol, water, and 1,2-hexanediol.

[0020] Secondly, this application provides the application of an acne-removing composition that blocks acne scars in the preparation of acne-removing cosmetics.

[0021] In other embodiments of this application, the volume concentration of the acne-removing composition for blocking acne marks in the aqueous solution is 0.0032%-2% by volume percentage.

[0022] Thirdly, this application provides a cosmetic product comprising the acne-removing composition for blocking acne scars provided in any of the first aspects.

[0023] In other embodiments of this application, the amount of maltodextrin added to the cosmetic is 0.5%-2.5% by mass percentage.

[0024] In other embodiments of this application, the amount of arginine added to the cosmetic is 0.2%-1.5% by mass percentage.

[0025] In other embodiments of this application, the amount of peony root extract added to the cosmetic is 1%-10% by mass percentage. Attached Figure Description

[0026] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0027] Figure 1 A diagram showing the skin condition of the subjects before they used the sample from Example 2;

[0028] Figure 2 This is a diagram of the skin condition of the subjects 3 days after using the sample from Example 2;

[0029] Figure 3 A diagram showing the skin condition of the subjects before they used the sample from Example 2;

[0030] Figure 4 This is a diagram of the skin condition of the subjects 3 days after using the sample from Example 2;

[0031] Figure 5 The ITA° values ​​were measured and analyzed by VISA imaging after subjects used Example 2 and a commercially available competing product for 7 days.

[0032] Figure 6 The L was measured and analyzed by VISA imaging after subjects used Example 2 and a commercially available competing product for 7 days. value;

[0033] Figure 7 The subjects used Example 2 and a commercially available competing serum for 7 days, and the results were analyzed using VISA imaging to test a. value;

[0034] Figure 8 This is a radar chart of sensory evaluation tests of the sample and commercially available product in Example 2. Detailed Implementation

[0035] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions in the embodiments of this application will be clearly and completely described below. Obviously, the described embodiments are some embodiments of this application, but not all embodiments.

[0036] Therefore, the following detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments of the application. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

[0037] Research has revealed that the formation mechanism of acne is complex, with inflammation playing a crucial role in its development and the formation of acne scars. Activation of inflammatory factors triggers vasodilation, alters blood circulation, increases vascular permeability, and stimulates melanocytes to produce large amounts of melanin, ultimately leading to acne scars. Further research has found that a key factor is reducing the transmission of TNF-α inflammatory factors during acne formation, minimizing their impact on blood vessels and melanocytes, inhibiting acne worsening, and preventing acne scar formation. This pathway provides direction for addressing the problems of existing products irritating sensitive skin and the lack of comprehensive, effective, and gentle acne scar removal solutions, and also lays the foundation for the development of novel acne scar removal compositions.

[0038] Based on this, the present application provides an acne-removing composition for blocking acne scars, wherein the acne-removing composition for blocking acne scars comprises, by weight parts:

[0039] Maltodextrin 0.5-2.5 parts, arginine 0.2-1.5 parts and peony root extract 1-10 parts.

[0040] In the above technical solution, maltodextrin, arginine, and peony root extract are combined to achieve a synergistic effect, effectively improving the treatment of acne and acne scars. The three ingredients work synergistically to reduce the transmission of TNF-α inflammatory factors, minimize the impact on blood vessels and melanocytes, inhibit acne worsening, and prevent acne scar formation, thus greatly enhancing the treatment of acne and acne scars. Applying this composition to cosmetics helps solve the problems of existing products irritating sensitive skin and failing to provide comprehensive, effective, and gentle treatment for acne and acne scars.

[0041] Further optionally, and exemplaryly, in some embodiments of this application, the acne-removing composition for blocking acne scars, by weight parts, includes: maltodextrin 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, 1.9, 2.0, 2.1, 2.2, 2.3, 2.4, 2.5 or any two of the aforementioned values; arginine 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9, 1.0, 1.1, 1.2, 1.3, 1.4, 1.5 or any two of the aforementioned values; and peony root extract 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 or any two of the aforementioned values.

[0042] In the above technical solution, maltodextrin, arginine, and peony root extract are combined to achieve a synergistic effect, effectively improving the effect of acne scar removal. Maltodextrin in the above composition is easily soluble in water, producing a clear and transparent aqueous solution with a pH value (10% aqueous solution) of 1.0-3.0. It has excellent moisturizing, antibacterial, and gentle effects in promoting keratin renewal and skin metabolism. When combined with other ingredients, it can regulate skin metabolism and inflammation, prevent excessive degradation of the extracellular matrix around hair follicles, maintain the integrity of hair follicle structure, and help reduce skin damage caused by acne, thus reducing the formation of acne scars. Simultaneously, since the occurrence of acne is closely related to inflammation, which stimulates excessive sebum secretion from the sebaceous glands and also causes inflammatory reactions in the tissues surrounding the hair follicles, the antioxidant effect of maltodextrin can alleviate these inflammatory reactions. It can reduce the infiltration of inflammatory cells around hair follicles and inhibit the release of inflammatory mediators such as interleukins (IL-1β, IL-6, etc.). Maltose can also affect pH and has a certain antibacterial effect, inhibiting pathogens that cause acne, such as Propionibacterium acnes, thus achieving the effect of acne removal from multiple aspects.

[0043] Furthermore, the arginine in the above composition, with a pH value (10% aqueous solution) of 10.0-12.0, has moisturizing and pH-regulating effects, providing nutrients to skin cells, promoting cell metabolism, and helping to repair damaged skin tissue. This helps improve skin texture, reduce fine lines and wrinkles, and make the skin firmer and smoother. The effect of arginine is manifested in its improvement of skin blood circulation. Acne is partly caused by blockage of the hair follicle sebaceous gland duct, resulting in poor blood circulation. The NO produced by arginine can dilate blood vessels and increase blood perfusion to the skin. This helps deliver nutrients and immune cells to the site of skin inflammation, accelerating the resolution of inflammation and significantly reducing acne scars. The above technical solution, by combining arginine with other ingredients, can be combined with maltose to regulate pH, improve antibacterial effects, and enhance the inhibition of inflammatory mediators.

[0044] Furthermore, the peony root extract in the above composition, with a 1% aqueous solution pH of 5.0-7.5, possesses skin-care effects such as moisturizing, anti-oxidation, and brightening. At a concentration of 2%, it inhibits hyaluronidase activity by 21.61±1.56%. Peony root extract can inhibit the activation of inflammatory cells such as macrophages and neutrophils, and reduce the release of inflammatory mediators such as prostaglandin E2 (PGE2) and histamine. Peony root extract contains high levels of active ingredients, such as the effective ingredient paeoniflorin. Paeoniflorin can regulate inflammation-related signaling pathways, such as the JAK-STAT (Janus kinase-signal transduction and transcription activator) pathway. Under inflammatory stimulation, the JAK-STAT pathway is activated, leading to the expression of a series of inflammatory factors, including factors that can induce TNF-α. Peony root extract indirectly reduces the formation of acne scars by inhibiting the overactivation of the JAK-STAT pathway and reducing the production of inflammatory factors. The peony root extract mentioned above can synergistically enhance the acne-removing effect by working with arginine and maltodextrin, targeting different points.

[0045] Further, optionally, in some embodiments of this application, the acne-removing composition for blocking acne scars, by weight, includes:

[0046] Maltodextrin 1.0-2.0 parts, arginine 0.40-1.0 parts and peony root extract 5-8 parts.

[0047] Furthermore, in some embodiments of this application, in the acne-removing composition for blocking acne scars, the mass ratio of maltodextrin to arginine is (2~5):1.

[0048] For example, in some embodiments of this application, in the acne-removing composition for blocking acne scars, the mass ratio of maltodextrin to arginine is 2:1, 2.2:1, 2.3:1, 2.4:1, 2.5:1, 2.6:1, 2.7:1, 2.8:1, 2.9:1, 3:1, 3.2:1, 3.5:1, 3.8:1, 4:1, 4.2:1, 4.5:1, 4.8:1, 5:1, or any range between any two of the aforementioned ratios.

[0049] In the above technical solution, by setting the mass ratio of maltodextrin to arginine in the acne-removing composition for blocking acne scars to (2~5):1, the pH value of the entire acne-removing composition for blocking acne scars can be 1.0-3.0; within this range, it is more conducive to blocking acne scars.

[0050] Further optionally, in some embodiments of this application, in the acne-removing composition for blocking acne scars, the mass ratio of maltodextrin to arginine is (2.5~4):1.

[0051] Further, alternatively, in some embodiments of this application, maltodextrin and arginine are added to the composition in powder form.

[0052] Further, alternatively, in some embodiments of this application, peony root extract is added to the composition in solution form.

[0053] Further, optionally, in some embodiments of this application, the peony root extract solution contains peony root extract and a solvent.

[0054] Further, alternatively, in some embodiments of this application, the solvents mentioned above include butanediol, water, and 1,2-hexanediol.

[0055] For example, in some embodiments of this application, the solvents mentioned above include butanediol, water, and 1,2-hexanediol.

[0056] For example, in some embodiments of this application, the composition of the peony root extract solution is: peony root extract, butylene glycol, water, and 1,2-hexanediol. The above solvents can be mixed in any proportion.

[0057] In the above-mentioned technical solution, by mass percentage, the peony root extract solution contains ≥8% peony root extract, with the remaining components being butylene glycol, hexanediol, and aqueous solution. In the above-mentioned technical solution, the peony root extract contains ≥1% paeoniflorin, an effective substance. Exemplarily, in some embodiments of this application, by mass percentage, the peony root extract solution contains 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, or any range between two of the aforementioned values.

[0058] For example, in some embodiments of this application, the paeoniflorin active ingredient in the peony root extract is 1%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, 2.6%, 2.7%, 2.8%, 2.9%, 3.0%, or any two of the aforementioned values, by mass percentage.

[0059] Furthermore, in some embodiments of this application, the pH value of the acne-removing composition for blocking acne scars is 3.0-5.5.

[0060] A low pH (3.0-5.5) can enhance the antibacterial activity of maltodextrin and inhibit Propionibacterium acnes; at the same time, an acidic environment helps promote keratin metabolism and unclog pores; in the above technical solution, the pH value of the acne-removing composition for blocking acne marks is 3.0-5.5; within this range, it is beneficial to block acne marks.

[0061] For example, in some embodiments of this application, the pH value of the acne-removing composition for blocking acne scars is 3.0, 3.1, 3.2, 3.5, 3.8, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5 or a range between any two of the aforementioned values.

[0062] Further optionally, in some embodiments of this application, the pH value of the acne-removing composition for blocking acne scars is 4.0-5.5.

[0063] Low pH can enhance the antibacterial activity of maltodextrin and inhibit Propionibacterium acnes; at the same time, an acidic environment helps promote keratin metabolism and unclog pores; under neutral to weakly acidic conditions, it can enhance the stability of paeoniflorin and combine with arginine to avoid acid-base neutralization leading to component inactivation. However, excessive arginine may lead to an increase in pH, weakening the antibacterial effect of maltodextrin, so the ratio needs to be controlled. Considering the above, the optimal pH value of the acne-removing composition for blocking acne scars in the above technical solution is 4.0-5.5. In this preferred pH range, the composition greatly enhances (1) antibacterial properties: the acidic environment inhibits the growth of Propionibacterium acnes (lower minimum inhibitory concentration MIC value); (2) keratin metabolism: promotes the shedding of old keratin and reduces hair follicle blockage; (3) stability: arginine and maltodextrin can form ion pairs in this pH range, enhancing transdermal absorption. It can greatly improve the efficacy of acne removal and acne scar blocking.

[0064] Furthermore, in some embodiments of this application, the optical rotation of arginine ranges from 26.0° to 27.9°.

[0065] In the above technical solution, the optical rotation range of arginine is 26.0°~27.9°; when it is combined with other ingredients, it is beneficial to the effect of the composition on removing acne scars.

[0066] For example, in some embodiments of this application, the optical rotation of arginine is in the range of 26.0°, 26.1°, 26.2°, 26.3°, 26.4°, 26.5°, 26.6°, 26.7°, 26.8°, 26.9°, 27.0°, 27.1°, 27.2°, 27.3°, 27.4°, 27.5°, 27.6°, 27.7°, 27.8°, 27.9° or any two of the aforementioned values.

[0067] Some embodiments of this application provide the use of an acne-removing composition that blocks acne scars in the preparation of acne-removing cosmetics.

[0068] Furthermore, in some embodiments of this application, the volume concentration of the acne-removing composition for blocking acne marks in the aqueous solution is 0.0032%-2% by volume percentage.

[0069] For example, in some embodiments of this application, the volume concentration of the acne-removing composition for blocking acne scars in the aqueous solution is 0.0032%, 0.0033%, 0.0034%, 0.0035%, 0.0036%, 0.0038%, 0.0040%, 0.0042%, 0.0045%, 0.0048%, 0.0050%, 0.0052%, 0.0055%, 0.01%, 0.05%, 0.1%, 0.5%, 1%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2%, or any two of the aforementioned values, by volume percentage.

[0070] Some embodiments of this application provide a cosmetic product comprising the acne-removing composition for blocking acne scars provided in any of the foregoing embodiments.

[0071] Furthermore, in some embodiments of this application, the amount of maltodextrin added to the cosmetic is 0.5%-2.5% by mass percentage.

[0072] For example, in some embodiments of this application, the amount of maltodextrin added to the cosmetic, by mass percentage, is 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 1.9%, 2.0%, 2.1%, 2.2%, 2.3%, 2.4%, 2.5%, or any two of the aforementioned values.

[0073] Further, optionally, in some embodiments of this application, the amount of arginine added to the cosmetic is 0.2%-1.5% by mass percentage.

[0074] For example, in some embodiments of this application, the amount of arginine added to the cosmetic, by mass percentage, is 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1.0%, 1.1%, 1.2%, 1.3%, 1.4%, 1.5%, or any two of the aforementioned values.

[0075] Further, optionally, in some embodiments of this application, the amount of peony root extract added to the cosmetic is 1%-10% by mass percentage.

[0076] For example, in some embodiments of this application, the amount of peony root extract added to the cosmetic, by mass percentage, is 1%, 1.2%, 1.3%, 1.4%, 1.5%, 1.6%, 1.7%, 1.8%, 2.0%, 2.5%, 3.0%, 3.5%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 9.5%, 10%, or any two of the aforementioned values.

[0077] Further, alternatively, in some embodiments of this application, the maltodextrin and arginine in the above-mentioned cosmetics are added to the cosmetics in powder form.

[0078] Further, alternatively, in some embodiments of this application, the peony root extract described above is added to the cosmetic in solution form.

[0079] Exemplary examples, in some embodiments of this application, the peony root extract solution comprises peony root extract, butylene glycol, water, and 1,2-hexanediol. Optionally, the peony root extract in the peony root extract solution comprises 80% or more by mass.

[0080] It should be noted that the 1%-10% addition of peony root extract in the aforementioned cosmetics is calculated based on the content of peony root extract; for example, when added to the cosmetics in the form of a peony root extract solution with a mass percentage of 80%, the mass percentage of the added peony root extract is 1.25%~12.5%.

[0081] Further optionally, in some embodiments of this application, the above-mentioned cosmetics also include: mandelic acid, nicotinamide, butylene glycol and 10-hydroxydecanoic acid, salicylic acid and betaine, sodium hydroxide, capryloyl hydroxamic acid and 1,3-propanediol and 1,2-hexanediol, xanthan gum, PEG-40 hydrogenated castor oil, allantoin, Melaleuca alternifolia leaf oil, disodium EDTA, and water.

[0082] Further optionally, in some embodiments of this application, the above-mentioned cosmetic further includes: 1%-5% mandelic acid, 1%-5% nicotinamide, 1%-5% butylene glycol and 10-hydroxydecanoic acid, 1%-5% salicylic acid and betaine, 0.5%-3% sodium hydroxide, 0.5%-5% capryloyl hydroxamic acid and 1,3-propanediol and 1,2-hexanediol, 0.1%-1.0% xanthan gum, 0.1%-5% PEG-40 hydrogenated castor oil, 0.01%-0.50% allantoin, 0.01%-0.10% Melaleuca alternifolia leaf oil, 0.01%-0.50% disodium EDTA, and the balance being water.

[0083] The features and performance of this application will be further described in detail below with reference to embodiments:

[0084] Examples and Comparative Examples

[0085] An acne-removing composition for preventing acne scars is provided, the composition of which is shown in Table 1.

[0086] Table 1

[0087]

[0088] Experimental Example

[0089] (1) Sample preparation

[0090] Acne-removing composition: Dilute each example or comparative sample with pure water to a volume fraction of 2%, then filter with a 0.22μm filter, and collect the filtrate as the sample stock solution.

[0091] Negative controls: basal culture medium (cell viability assay); basal culture medium containing LPS (TNF-α inflammatory factor content assay).

[0092] (2) Experimental operation procedures

[0093] Cell viability assay: RAW264.7 cells were seeded in 96-well plates. After 24 hours, the culture medium was aspirated, and basal medium containing different concentrations of the test samples was added. After another 24 hours, the OD490 mm was measured using the MTT assay, and the effect of the test samples on the viability of RAW264.7 cells was analyzed using a t-test. (RAW264.7 is a mouse mononuclear macrophage cell line with typical macrophage functional characteristics, capable of mimicking key immune processes in skin inflammatory responses).

[0094] This is embodied through the aforementioned technical solutions.

[0095] TNF-α inflammatory factor content assay: RAW264.7 cells were seeded in 96-well plates and cultured for 24 h. The culture medium was then replaced with basal medium containing different concentrations of the experimental samples. After 24 h, the cell supernatant was collected and centrifuged. The supernatant was then aspirated, and the TNF-α content in the cell culture supernatant was measured using an ELISA kit to determine the effect of the experimental samples on the TNF-α content of macrophages.

[0096] (3) Calculation formula

[0097] Relative TNF-α content (%) = (TNF-α content in sample / TNF-α content in negative control) 100%

[0098] Cellular testing was conducted to verify the anti-inflammatory effects of the composition.

[0099] Before testing the TNF-α inflammatory factor content in the sample cells, cell viability screening was performed to determine the optimal test concentration.

[0100] (4) Detection of TNF-α inflammatory factor content in cells

[0101] After screening for cell viability and determining the optimal concentration (0.0032%), the TNF-α inflammatory factor content in RAW264.7 cells was measured to determine the effect of the sample on the TNF-α inflammatory factor content in RAW264.7 cells.

[0102] Following the laboratory method (HMC-WI-052 in vitro TNF-α inflammatory factor content determination), the overall cell activity and cellular TNF-α inflammatory factor content were tested when the sample concentration was 0.0032%.

[0103] The test results are shown in Table 2.

[0104] Table 2

[0105]

[0106] As can be seen from the data in the table above, the 0.0032% test sample has a significant effect on inhibiting the content of TNF-α inflammatory factor in cells (statistical P<0.0001). That is, at a concentration of 0.0032%, the test sample of the examples has a significant ability to inhibit the content of TNF-α inflammatory factor in cells, while the comparative examples have a less effective ability to inhibit the content of TNF-α inflammatory factor in cells than Examples 1-3.

[0107] Among them, Comparative Example 4 showed poor cell viability. The toxicity test of maltobionic acid alone showed that the cytotoxic risk of maltobionic acid was controllable. This indicates that in Comparative Example 4, the lower pH under high concentration of maltobionic acid had a greater impact on cell viability, because low pH may damage cell membrane stability, interfere with enzyme activity and cell metabolism, leading to cell death or abnormal function. This suggests that Comparative Example 4 may have increased the content of TNF-α inflammatory factor.

[0108] Experiment Example 2: Application of the Product

[0109] Cosmetics were made using the compositions provided in the foregoing examples and comparative examples. The composition and proportions of the cosmetics are shown in Table 3.

[0110] Table 3 (wt%)

[0111]

[0112] The performance of each of the above cosmetic samples was tested:

[0113] 1. Skin occlusive patch test

[0114] Test samples: Samples from Examples 1-3 and Comparative Examples 1-4, totaling 7 samples, were tested on 14 volunteers with oily, acne-prone, and sensitive skin. Each volunteer was tested on a different sample. Test site: Normal skin on the flexor surface of the forearm.

[0115] The specific steps are as follows: Clean the skin at the test site, take 0.025g of sample, place it in the small chamber of the spot tester (each tester has 10 chambers, that is, 5 points per person), stick the skin spot test paper firmly and flat from bottom to top, and press it gently with your hand a few times to expel air.

[0116] Patch test duration: 24 hours.

[0117] Observation time: 24 hours after application, remove the test material from the skin spot and observe the results at least 30 minutes later.

[0118] Result judgment:

[0119] Phenomenon 1: (+-)- Suspicious reaction: Only faint (unclear) erythema.

[0120] Phenomenon 2: (+) -- Weak (non-bubble) positive reaction: erythema, infiltration, possibly small red rashes.

[0121] Phenomenon 3: (++) -- Strong (vesicular) positive reaction: erythema, infiltration, papules, not small vesicles.

[0122] Phenomenon 4: (+++) -- Extremely positive reaction: redness and swelling with large blisters.

[0123] Phenomenon 5: (-) -- No response.

[0124] The results are shown in Table 4.

[0125] Table 4

[0126]

[0127] Positive rate (%) = Sum of phenomena (1-5) / Total number of people 100%

[0128] Table 4 shows the test results. Among the subjects in Examples 1-3 and Comparative Examples 1-4, Comparative Examples 2, 3, and 4 showed suspicious reactions, while the rest did not show suspicious or positive reactions. According to the "Cosmetic Safety Technical Specifications" (2015 edition), this test substance will not cause adverse skin reactions in this batch of subjects. This indicates that the acne-removing composition for blocking acne scars provided in the examples of this application is mild and safe, does not irritate the skin, and is suitable for sensitive, oily, and acne-prone skin.

[0129] 2. Sensitive, oily, acne-prone skin a - Short-term effect test on acne treatment, smoothing, pore minimizing, brightening, and reducing redness on cheeks.

[0130] For efficacy testing on sensitive, oily, and acne-prone skin, a human trial was conducted. A specific experimental population was formed as the test group. Changes in the surface skin of the subjects' faces before and after using the sample were measured using a polarized light source to evaluate sample a. -Effects acne treatment, smoothing, minimizing pores, brightening, and reducing redness on cheeks.

[0131] A total of 10 participants with oily and acne-prone skin were included in the study. The testing period lasted for 3 days, during which participants applied the sample to their face once daily. Two samples were used on each participant's left and right cheeks for testing.

[0132] Experimental instrument: Skin imaging system

[0133] (1) The images obtained using the above-mentioned experimental instruments are shown in the attached instruction manual. Figures 1-4 .

[0134] in, Figure 1 , Figure 3The skin condition of the subjects before using the sample of Example 2 shows that there were obvious pimples, severe erythema, and large acne scars. Figure 2 , Figure 4 The results show the skin condition of the subjects after using the sample of Example 2 for 3 days. It can be seen that the acne area was significantly reduced, the skin erythema was significantly improved, the acne scar area was also greatly reduced, and there was no allergic reaction. This indicates that the cosmetic with the acne-removing composition that blocks acne scars of this application can effectively improve the problems of acne, erythema and acne scars of oily, acne-prone and sensitive skin, and has low irritation.

[0135] (2) Data was exported from the skin testing instrument, and the instrument test analysis data is as follows:

[0136] Table 5

[0137]

[0138] The experimental results in the table above show that:

[0139] 1. a - Acne treatment: The area of ​​redness in acne decreased by 12.15%, indicating effective improvement in acne redness and swelling. 2. Skin smoothness in acne-affected areas increased by 25%, indicating a good effect on smoothing closed comedones. 3. Enlarged pores on the face decreased by 17.24%, indicating an effect on refining pores. 4. Facial skin brightness improved by 5.32%, indicating a good effect on improving red and dark acne marks. 5. The area of ​​redness on the cheeks decreased by 6.26%, indicating a good effect on improving erythema, preventing inflammation, and reducing the possibility of leaving marks from erythema.

[0140] This fully demonstrates that the composition of this application can achieve the effects of acne removal and acne scar removal from different angles and in multiple ways.

[0141] 3. Comparison with competitor testing

[0142] We selected several well-known commercially available products with good acne scar fading effects, including a certain essence from Oushiman (a product with strong acne scar fading and dark spot removal effects, based on market reviews). We compared these products with six participants with oily, acne-prone, and sensitive skin. The tests were conducted over seven days to assess the effects on acne and acne scar fading. The ITA° value and a were analyzed using VISA imaging. The values ​​are calculated by taking the average value, which corresponds to the improvement of acne scars and the reduction of redness in acne, respectively.

[0143] For efficacy testing on sensitive, oily, and acne-prone skin, a human trial was conducted. A specific experimental group comprised the test subjects. Changes in the skin surface before and after using the sample were measured using a polarized light source. Test values, including ITA° value and L, were recorded. value, a Values ​​are used to evaluate the fading of acne scars, acne marks, and a in samples. - Improvement of redness on acne-prone cheeks. Test results are shown in Table 6 below and attached to the instruction manual. Figures 5-7 :

[0144] Table 6

[0145]

[0146] From Table 6 above and the appendix to the instruction manual Figures 5-7 It can be seen that:

[0147] 1. ITA° reported a 3.00% increase in skin brightness in areas with faded acne scars, indicating a significant improvement in the color of these areas. 2. L The feedback value assesses the change in skin brightness, i.e., the improvement effect on acne scars. A 1.00% increase in skin brightness indicates a significant improvement in red and dark acne scars compared to competing products. 3. a The feedback value reflects the area of ​​redness on the cheeks, i.e., the improvement in the acne area. It decreased by 3.04%, indicating that it significantly improves the redness on the cheeks compared to competing products.

[0148] Based on comprehensive experimental data, it is shown that compared with competing products, this product has a better effect on improving erythema, intercepting the production of inflammation, and reducing the marks left by skin erythema.

[0149] 4. Sensory evaluation test

[0150] The samples prepared in Example 2 were tested, and a blind sensory evaluation was conducted by 10 professional evaluators. The evaluators used the product continuously for 7 days, and the test items included rapid absorption and non-stickiness, skin hydration, fading of skin marks, improvement of enlarged pores, improvement of skin erythema, product irritation, and overall evaluation. The scores for each test were statistically analyzed, with a total score of 10 points. A higher score indicates a higher level of acceptance of the product by the test subjects.

[0151] The scoring results are shown in Table 7 below.

[0152] Table 7

[0153]

[0154] The test results above show that the acne-removing composition for preventing acne scars presented in this application received high sensory approval from the testers. The average data for the test items in the table above was calculated, and test data for the aforementioned commercially available acne-removing products (such as a certain essence from Oushiman) were retrieved from historical databases and compared using a radar chart. The results are as follows. Figure 8 As shown. From Figure 8It is evident that the acne-removing composition of this application, which has the function of blocking acne scars, has a higher sensory appeal than existing products.

[0155] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. An acne-removing composition for preventing acne scars, characterized in that, The acne scar-blocking composition comprises, by weight parts: Maltodextrin 0.5-2.5 parts, arginine 0.2-1.5 parts and peony root extract 1-10 parts.

2. The acne-removing composition for preventing acne scars according to claim 1, characterized in that, The acne scar-blocking composition comprises, by weight parts: Maltodextrin 1.0-2.0 parts, arginine 0.40-1.0 parts and peony root extract 5-8 parts.

3. The acne-removing composition for preventing acne scars according to claim 1, characterized in that, In the acne-removing composition for blocking acne scars, the mass ratio of maltodextrin to arginine is (2~5):

1.

4. The acne-removing composition for preventing acne scars according to claim 1, characterized in that, In the acne-removing composition for blocking acne scars, the mass ratio of maltodextrin to arginine is (2.5~4):

1.

5. The acne-removing composition for blocking acne scars according to any one of claims 1-4, characterized in that, The pH value of the acne-removing composition for blocking acne scars is 3.0-5.

5.

6. The acne-removing composition for blocking acne scars according to any one of claims 1-4, characterized in that, The pH value of the acne-removing composition for blocking acne scars is 4.0-5.

5.

7. The acne-removing composition for blocking acne scars according to any one of claims 1-4, characterized in that, The optical rotation of the arginine is in the range of 26.0° to 27.9°.

8. The acne-removing composition for blocking acne scars according to any one of claims 1-4, characterized in that, The peony root extract is added to the composition in solution form.

9. The acne-removing composition for preventing acne scars according to claim 8, characterized in that, By mass percentage, the peony root extract solution contains ≥8% peony root extract.

10. The acne-removing composition for preventing acne scars according to claim 8, characterized in that, By mass percentage, the paeoniflorin content in the peony root extract is ≥1%.

11. The acne-removing composition for preventing acne scars according to claim 8, characterized in that, The peony root extract solution contains peony root extract and solvents; the solvents include butanediol, water, and 1,2-hexanediol.

12. The use of the acne-removing composition for blocking acne scars according to any one of claims 1-11 in the preparation of acne-removing cosmetics.

13. The application according to claim 12, characterized in that, The volume concentration of the acne-removing composition for blocking acne scars in the aqueous solution is 0.0032%-2% by volume percentage.

14. A cosmetic product, characterized in that, The cosmetic product includes the acne-removing composition for blocking acne scars as described in any one of claims 1-11; The amount of maltodextrin added to the cosmetic product is 0.5%-2.5% by weight. The amount of arginine added to the cosmetic product is 0.2%-1.5% by weight. The amount of peony root extract added to the cosmetic product is 1%-10% by weight.

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