Sunscreen composition, application thereof and skin care product

Abstract

The invention belongs to the technical field of cosmetics, and discloses a sunscreen composition, which is prepared from the following components in parts by mass: 20 to 35 parts of pyracantha fortuneana fruit extract, 65 to 80 parts of modified silk coated sunscreen agent and 10 to 15 parts of coated freshener. Through the synergistic effect of the pyracantha fortuneana fruit extract, the modified silk wrapped sun-screening agent and the wrapped freshener, the sunscreen cream can play more efficient sun-screening, anti-oxidation, anti-aging and black fading effects, and is mild and non-irritating to skin. Meanwhile, the invention further discloses application of the sunscreen composition and a skin care product.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and discloses a sunscreen composition and application thereof, and a skin care product. Background Art

[0002] As people's understanding of the hazards of ultraviolet (UV) radiation continues to deepen, sun protection has become a key part of daily skin care and protection. Ultraviolet rays, especially medium-wave ultraviolet rays (UVB, wavelength 280-320nm) and long-wave ultraviolet rays (UVA, wavelength 320-400nm), can penetrate the atmosphere and cause various damages to the skin. UVB can cause sunburn, erythema, and inflammation of the skin, and long-term exposure can increase the risk of skin cancer; UVA can penetrate into the dermis of the skin, destroy collagen and elastic fibers, accelerate skin aging, and cause wrinkles, sagging, and pigmentation. Therefore, sunscreen products are also receiving more and more attention.

[0003] There are many types of existing sunscreen products, mainly including chemical sunscreens and physical sunscreens. Physical sunscreen products are often thick in texture, which may make the skin look white after application, and consumers have a bad experience of using them; chemical sunscreen products are more irritating to the skin, may cause skin allergic reactions, and are not friendly to people with sensitive skin. Prior art CN115869201A discloses a sunscreen composition with moisturizing ability and a preparation method, the composition includes a first phase, a second phase and a third phase, the first phase includes diethylaminohydroxybenzoyl hexyl benzoate and phenethyl benzoate, the second phase includes ethanol and essence, and the third phase includes a silk protein solution encapsulating octocrylene, a silk protein solution encapsulating ethylhexyl methoxycinnamate, glycerol, butylene glycol, trehalose, glyceryl glucoside, PEG-7 glyceryl cocoate, a mixture of phenoxyethanol and 1,2-octanediol, and water. It uses silk protein to wrap sunscreen, avoiding the harm of sunscreen to the skin; further observation of this solution shows that although it can reduce the irritation and harm of sunscreen to the skin, after using it for a period of time, it cannot avoid the problem of decreased sunscreen effect, and it cannot play a good repair effect on the damage caused by ultraviolet rays.

[0004] Therefore, the technical problem to be solved by the present invention is: how to obtain a sunscreen composition which is mild and non-irritating to the skin, has strong durability, and has a repairing effect on the skin. Summary of the invention

[0005] The object of the present invention is to provide a sunscreen composition, comprising a pyracantha fruit extract, a modified silk-wrapped sunscreen agent, and a wrapped cooling agent. Through the synergistic effect of the three, it can exert more efficient sunscreen, anti-oxidation, anti-aging, and melanin-fading effects while being gentle and non-irritating to the skin.

[0006] At the same time, the invention also provides the use of the sunscreen composition and skin care products.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A sunscreen composition comprises the following components: a pyracantha fruit extract and a modified silk-wrapped sunscreen agent, wherein the mass portion of the pyracantha fruit extract is 20 to 35 parts, and the mass portion of the modified silk-wrapped sunscreen agent is 65 to 80 parts.

[0009] In some studies of the present invention, it is found that the active components of flavonoids and tannins in the extract of Pyracantha fruit can effectively remove oxidative free radicals caused by ultraviolet rays in cells and improve the collagen damage caused by ultraviolet radiation to the skin; modified silk protein encapsulation technology is used to control the degree of cross-linking to construct a three-dimensional confined space, stably bind chemical sunscreen molecules in the carrier network to avoid harm to the skin, and use the viscoelasticity of silk protein to form a directional protective film on the surface of the skin, which can be verified by in vitro transdermal experiments to achieve targeted retention in the stratum corneum. The tannins in the extract of Pyracantha fruit and silk protein form a porous mesh membrane through non-covalent cross-linking (hydrogen bonding / hydrophobic interaction). The network has a molecular sieve effect, allowing small molecules (such as flavonoids) to diffuse freely, while trapping silk microcapsules. The low molecular weight antioxidants in the extract of Pyracantha fruit penetrate quickly, while the porous mesh membrane is slowly formed, the medium molecular weight active substances are slowly released through the network pores, and the silk microcapsules stay in the stratum corneum to continuously absorb ultraviolet rays.

[0010] Preferably, the sunscreen composition further comprises 10 to 15 parts by weight of an encapsulated cooling agent.

[0011] In further research of the present invention, it was found that encapsulating the cooling agent can promote the transmission of cooling ingredients to the sensory nerve endings in the lower epidermis, and these cooling ingredients are slowly released from the inner core, thereby obtaining a lasting refreshing feeling without irritation to the skin, calming the skin, quickly fading redness, and relieving pain after sun exposure; the modified silk protein shell and the shell encapsulating the cooling agent have an interface effect, the silk protein β-folding structure and the amide group of the shell encapsulating the cooling agent form a π-π stack, the submicron microsphere surface and the silk microcapsule produce a mechanical interlocking effect, and synergistically and stably reside on the skin surface to form a composite structure of an outer layer of sunscreen agent-an inner layer of cooling agent, effectively increasing the durability of the sunscreen product, and synergistically forming a porous mesh membrane formed by the pyracantha fruit extract and the silk microcapsule to form a stable complex, which on the one hand enhances the retention capacity of the silk protein microcapsule-cooling agent composite structure, and further, the microsphere shell gradually dissolves under the triggering of skin temperature (32°C), and releases the cooling agent in segments, and such a molecular cross-linking system enhances the stability of the sustained-release system.

[0012] More preferably, the specific components of the modified silk-wrapped sunscreen include, by mass percentage: 10wt% to 70wt% sunscreen, 5wt% to 10wt% polysilicone-14, 2wt% butylene glycol, 2wt% phenoxyethanol, 0.2wt% EDTA-2Na, and the remainder water.

[0013] More preferably, the sunscreen is at least one of ethylhexyl methoxycinnamate, octocrylene, butyl methoxydibenzoylmethane, and diethylaminohydroxybenzoyl hexyl benzoate.

[0014] Preferably, the specific ingredients of the encapsulated cooling agent include, by mass percentage: 2.5wt% to 7wt% menthol lactate, 2.5wt% to 7wt% methyl menthane carboxamide, 2.5wt% to 7wt% methyl diisopropyl amide, 2.5wt% to 7wt% hydroxypropyl cellulose, 1wt% to 5wt% isopropyl palmitate, 0.3wt% to 0.75wt% phenoxyethanol, 0.3wt% to 0.75wt% ethylhexylglycerin, and the balance is propylene glycol.

[0015] In addition, the present invention also discloses the use of the sunscreen composition as described above in preparing skin care products.

[0016] Preferably, the skin care product is a skin care product for sun protection.

[0017] Finally, the present invention also discloses a skin care product, wherein the skin care product contains 10 wt % to 40 wt % of the sunscreen composition described above.

[0018] Preferably, the skin care products include lotions, creams, sprays, essences, essential oils, cleansers, lotions, facial masks, and gels.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] The sunscreen composition provided by the present invention comprises a pyracantha fruit extract, a modified silk-wrapped sunscreen agent, and a wrapped cooling agent.

[0021] Pyracantha fruit extract contains active ingredients of flavonoids and tannins, which can effectively remove oxidative free radicals caused by ultraviolet rays in cells and improve collagen damage caused by ultraviolet radiation to the skin; modified silk protein encapsulation technology is used to control the degree of cross-linking to construct a three-dimensional confined space, stably bind chemical sunscreen molecules in the carrier network to avoid harm to the skin, and use the viscoelasticity of silk protein to form a directional protective film on the skin surface. In vitro transdermal experiments have verified that it can achieve targeted retention in the stratum corneum. The tannins in the Pyracantha fruit extract and silk protein form a porous mesh membrane through non-covalent cross-linking (hydrogen bonding / hydrophobic interaction). The network has a molecular sieve effect, allowing small molecules (such as flavonoids) to diffuse freely while trapping silk microcapsules. The low molecular weight antioxidants in the Pyracantha fruit extract penetrate quickly, while the porous mesh membrane is slowly formed, and the medium molecular weight active substances are slowly released through the network pores, and the silk microcapsules stay in the stratum corneum to continuously absorb ultraviolet rays. The modified silk protein shell has an interface effect with the shell that wraps the cooling agent. The β-folded structure of silk protein and the amide group of the shell that wraps the cooling agent form a π-π stack. The submicron microsphere surface and the silk microcapsule produce a mechanical interlocking effect, which synergistically and stably reside on the skin surface to form a composite structure of an outer layer of sunscreen agent and an inner layer of cooling agent, effectively increasing the durability of sunscreen products. The porous mesh membrane formed by the synergistic Pyracantha fruit extract and the silk microcapsule forms a stable complex. On the one hand, the retention capacity of the silk protein microcapsule-cooling agent composite structure is enhanced. Furthermore, the microsphere shell gradually dissolves under the triggering of skin temperature (32°C) and releases the cooling agent in segments. Such a molecular cross-linking system enhances the stability of the sustained-release system. DETAILED DESCRIPTION

[0022] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] Product information:

[0024] Package coolant: the trade name is (HydroSal TM 2Salcool), purchased from Dongfeng Chemical (Hangzhou) Co., Ltd., which includes, by mass percentage: 5wt% menthol lactate, 5wt% methyl menthane carboxamide, 5wt% methyl diisopropyl amide, 5wt% hydroxypropyl cellulose, 3wt% isopropyl palmitate, 0.5wt% phenoxyethanol, 0.5wt% ethylhexylglycerin, and the balance propylene glycol.

[0025] Silk protein-wrapped sunscreen 1: The trade name is Silasoma MEA (S), the mass ratio of its shell material to the content is 10:90, and the content includes, by mass percentage: 40wt% octocrylene, 13wt% butyl methoxydibenzoylmethane, 7wt% polysilicone-14, 2wt% butylene glycol, 2wt% phenoxyethanol, 0.2wt% EDTA-2Na, and the balance water.

[0026] Silk protein-wrapped sunscreen 2: The trade name is Silasoma EP (S), and the mass ratio of its shell material to the content is 10:90. The contents include, by mass percentage: 3wt% ethylhexyl methoxycinnamate, 7wt% diethylamine hydroxybenzoyl hexyl benzoate, 5wt% polysilicone-14, 2wt% butylene glycol, 2wt% phenoxyethanol, 0.2wt% EDTA-2Na, and the balance water.

[0027] Silk protein-wrapped sunscreen 3: The trade name is Silasoma REA (E), and the mass ratio of its shell material to the content is 10:90. The content includes, by mass percentage: 40wt% octocrylene, 30wt% butyl methoxydibenzoylmethane, 10wt% polysilicone-14, 2wt% butylene glycol, 2wt% phenoxyethanol, 0.2wt% EDTA-2Na, and the balance water.

[0028] Pyracantha fruit extract: trade name Phytocine TM NOVO, purchased from BASF, comprises, by mass percentage, 85-95 wt % maltodextrin and 5-15 wt % pyracantha fruit extract.

[0029] Butyl methoxydibenzoylmethane, octocrylene, diethylaminohydroxybenzoyl hexyl benzoate: purchased from Chenghe Cosmetics Chemicals (Shanghai) Co., Ltd.;

[0030] Ethylhexyl methoxycinnamate: purchased from Guangzhou Kesa Road Trading Co., Ltd.;

[0031] Menthyl lactate: purchased from Guangzhou Minghui Chemical Technology Co., Ltd.

[0032] Example

[0033] The formula of the sunscreen composition of each embodiment and comparative example is shown in Table 1;

[0034] Table 1 Sunscreen composition formula (mass ratio)

[0035]

[0036]

[0037] Among them, the modified silk-wrapped sunscreen agent of Examples 1 to 7 is modified silk-wrapped sunscreen agent 1.

[0038] The chemical sunscreens of Comparative Examples 1, 3 and 4 consist of 75.5 wt % of octocrylene and 24.5 wt % of butyl methoxydibenzoylmethane.

[0039] Example 8

[0040] It is generally the same as Example 1, except that the modified silk-wrapped sunscreen agent is modified silk-wrapped sunscreen agent 2.

[0041] Example 9

[0042] It is generally the same as Example 1, except that the modified silk-wrapped sunscreen agent is modified silk-wrapped sunscreen agent 3.

[0043] The compositions obtained in the above-mentioned embodiments and comparative examples were prepared into sunscreen lotions according to the formula in Table 2, and the specific steps were as follows:

[0044] (1) Add cyclopentasiloxane, dibutyl adipate, and polyacrylate cross-linked polymer-6 into an oil phase pot, stir and heat to 90° C., and keep warm for later use;

[0045] (2) Add water, butanediol, p-hydroxyacetophenone, and 1,2-hexanediol into a water phase pot, stir and heat to 80°C, stir and dissolve, and keep warm for later use;

[0046] (3) The material in the oil phase pot is pumped into the water phase pot, homogenized for 5 minutes, stirred and cooled to 30° C., other materials and the sunscreen composition are added, stirred for 10 minutes, and then discharged.

[0047] Table 2 Sunscreen lotion formula

[0048] raw material Content (wt%) water TO 100 Cyclopentasiloxane 5 Dibutyl adipate 6 Ethanol 8 Sunscreen composition 30 Butanediol 3 Polyacrylate Crosspolymer-6 1 Aminomethyl propanol 0.1 p-Hydroxyacetophenone 0.3 1.2-Hexanediol 0.6 Phenoxyethanol 0.4

[0049] The sunscreen lotions corresponding to Examples 1 to 9 are Sunscreen Lotions 1 to 9, and the sunscreen lotions corresponding to Comparative Examples 1 to 4 are Sunscreen Lotions 10 to 13.

[0050] Performance Testing

[0051] Part I

[0052] Skin patch testing

[0053] Taking the 2015 "Technical Specification for Safety of Cosmetics" as the reference standard, the cosmetic irritation evaluation was conducted on Sunscreen Lotion 1, Sunscreen Lotion 4, Sunscreen Lotion 10 and Sunscreen Lotion 11. The test method was a skin patch test, and the test was conducted on 30 randomly distributed people aged 16 to 65 years old.

[0054] Test method: Put the test substance into the patch tester, the dosage is 0.020g~0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test. The test results refer to Table 3.

[0055] Evaluation criteria:

[0056] Grade 0: negative reaction;

[0057] Grade 1: Suspicious reaction, only slight erythema;

[0058] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0059] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0060] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and reaction beyond the test area.

[0061] Table 3 Skin patch test results

[0062] Sample source 24h 48h 72h Sunscreen 1 0 0 0 Sunscreen 4 0 0 0 Sunscreen 10 2 2 1 Sunscreen 11 1 1 1

[0063] It can be seen from Table 3 that sunscreen lotion 1 and sunscreen lotion 4 both contain Pyracantha fortuneana fruit extract and modified silk to encapsulate sunscreen, and the results for 24h, 48h and 72h are all negative, while sunscreen lotion 11 and sunscreen lotion 12 do not use modified silk to encapsulate the sunscreen. It can be seen that after applying the patch, weak erythema appeared at 24h, 48h and 72h, and sunscreen lotion 11 even showed erythema, infiltration, edema and the like, which is very likely that the sunscreen penetrated into the skin and caused adverse reactions. This shows that the composition of the technical solution of the present invention is safe and non-irritating to the skin, can effectively prevent the sunscreen from penetrating into the skin and causing reactions, and is highly safe.

[0064] SPF value determination

[0065] According to the 2015 edition of the "Technical Specifications for Safety of Cosmetics", Chapter 8, Section 2, Sunscreen Cosmetics Sun Protection Index (SPF Value), the sunscreen lotion's sun protection index (SPF value) was measured, and sunscreen lotions 1 to 13 were tested. The test results are shown in Table 4:

[0066] Table 4 SPF data table

[0067] Sample source SPF value Sunscreen 1 43 Sunscreen 2 38 Sunscreen 3 42 Sunscreen 4 55 Sunscreen 5 52 Sunscreen 6 48 Sunscreen 7 44 Sunscreen 8 39 Sunscreen 9 43 Sunscreen 10 29 Sunscreen 11 32 Sunscreen 12 27 Sunscreen 13 30 Sunscreen 14 1

[0068] From the analysis of Table 4, it can be seen that sunscreen lotions 1 to 9 all have a relatively high sun protection index. Among them, the sunscreen compositions of sunscreen lotions 4 to 6 are further enhanced by adding encapsulated cooling agents on the basis of sunscreen lotions 1 to 3, and the effect thereof is further enhanced. The SPF value of sunscreen lotion 4 is as high as 55.

[0069] When sunscreen lotion 15 uses pyracantha fruit alone, it has almost no sunscreen effect; sunscreen lotion 11 uses modified silk to encapsulate sunscreen alone, and its SPF value is 32. When sunscreen lotion 1 uses the two together, its sunscreen effect is qualitatively improved.

[0070] Sunscreen 12 uses unwrapped ethylhexyl methoxycinnamate sunscreen alone, and its SPF value is 27. When sunscreen 10 is used in combination with Pyracantha fortuneana fruit extract on the basis of sunscreen 12, the SPF value is 29, indicating that Pyracantha fortuneana fruit extract and ordinary ethylhexyl methoxycinnamate sunscreen do not have a synergistic effect, and the sunscreen effect is much lower than that of sunscreen 1; Sunscreen 13 adds ordinary cooling agent menthol lactate on the basis of sunscreen 10, and its sunscreen effect is only slightly improved compared with sunscreen 10; the value of sunscreen 7 is close to that of sunscreen 1, indicating that there is no synergistic effect between the unwrapped cooling agent and Pyracantha fortuneana fruit extract and modified silk-wrapped sunscreen.

[0071] The above description shows that only when Pyracantha fruit extract and modified silk-wrapped sunscreen are used together, there is a synergistic effect. Further adding a wrapped cooling agent also has a synergistic effect, maximizing the sunscreen effect. The tannins in the Pyracantha fruit extract and silk protein form a porous mesh membrane through non-covalent cross-linking (hydrogen bonding / hydrophobic interaction). The network has a molecular sieve effect, allowing small molecules (such as flavonoids) to diffuse freely while trapping silk microcapsules. The low molecular weight antioxidants in the Pyracantha fruit extract penetrate quickly, while the porous mesh membrane is slowly formed, and the medium molecular weight active substances are slowly released through the network pores. The silk microcapsules stay in the stratum corneum and continue to absorb ultraviolet rays. The modified silk protein shell has an interface effect with the shell that wraps the cooling agent. The β-folded structure of silk protein and the amide group of the shell that wraps the cooling agent form a π-π stack. The submicron microsphere surface and the silk microcapsule produce a mechanical interlocking effect, which synergistically and stably reside on the skin surface to form a composite structure of an outer layer of sunscreen agent and an inner layer of cooling agent, effectively increasing the durability of sunscreen products. The porous mesh membrane formed by the synergistic Pyracantha fruit extract and the silk microcapsule forms a stable complex. On the one hand, it enhances the retention capacity of the silk protein microcapsule-cooling agent composite structure. Furthermore, the microsphere shell gradually dissolves under the triggering of skin temperature (32°C) and releases the cooling agent in segments. Such a molecular cross-linking system enhances the stability of the sustained-release system and exerts the most efficient sunscreen effect.

[0072] Part 2

[0073] Because the composition containing encapsulated cooling agents, ethylhexyl methoxycinnamate, and menthol lactate is not suitable for direct action on cells, cell tests were not performed;

[0074] Determination of intracellular melanin content

[0075] B16-F10 cells in the logarithmic growth phase were selected, digested with 0.25% trypsin, and inoculated into 6-well culture plates with 2 mL of culture medium per well. The plates were placed at 37°C and 5% CO 2 Incubator. On the second day after inoculation, add the test solution and α-MSH (1 μM), and set up a control group (containing only 1 μM α-MSH), 2 mL per well. 37°C, 5% CO 2 After incubation for 48 hours, the cells were harvested, 1 mL of 1 mol / L NaOH solution (containing 10% by mass DMSO) was added, and the cells were incubated in a water bath at 80° C. for 30 minutes, and then the absorbance was measured at 490 nm using an ELISA reader. The test results are shown in Table 5.

[0076] According to preliminary tests, the best concentration of active ingredients in the test of intracellular melanin content is 0.3%. The compositions obtained in Example 1, Comparative Example 2 and Comparative Example 5 of the present invention are diluted with water to this concentration for testing.

[0077] Table 5 Melanin content

[0078] Sample source Melanin content / % Example 1 65.31 Comparative Example 2 90.47 Comparative Example 5 86.53 Control group 100

[0079] The data analysis of Table 5 shows that in terms of inhibiting melanin, the composition of Example 1 has an excellent inhibitory effect on melanin, and the melanin content is only 65.31%; the composition of Comparative Example 2 contains only the component of modified silk wrapped sunscreen. While the modified silk wraps the sunscreen, its silk protein also has a certain inhibitory effect on melanin, but the effect is small. The melanin content of Comparative Example 2 is 90.47%. The composition of Comparative Example 5 contains only Pyracantha fortuneana fruit extract, and its melanin content is 89.53%. Theoretically, the inhibitory effect of Comparative Example 2 is The inhibitory effect of Comparative Example 5 should be close to that of Example 1, but after verification, its melanin inhibition rate is 23%, and its inhibitory effect is much lower than 34.69% of Example 1, indicating that when the Pyracantha fruit extract and the modified silk-wrapped sunscreen are used together, a significant synergistic effect is exerted, and a more efficient inhibitory effect on melanin is achieved, which exceeds the theoretical effect; the tannins in the Pyracantha fruit extract and the silk protein are non-covalently cross-linked to form a porous mesh membrane, which has a molecular sieve effect, allowing small molecules (such as flavonoids) to diffuse freely, while trapping silk microcapsules. The low molecular weight antioxidants in the Pyracantha fruit extract penetrate quickly, while the porous mesh membrane is slowly formed, and the medium molecular weight active substances are slowly released through the network pores, so that the sunscreen composition has a better sunscreen effect.

[0080] Effects of the assay composition on reactive oxygen species levels in cells

[0081] NHDF cells were inoculated into 6-well culture plates with 2 mL of culture medium per well and placed in a 37°C, 5% CO 2 Incubate in an incubator for 12 hours. Then add the test solution and incubate for 72 hours. 2 UVA irradiation was performed for 30 min. After irradiation, the cells were harvested and the reactive oxygen species level was detected using a DCFH-DA probe. The detection results are shown in Table 6.

[0082] According to preliminary tests, in the test of cell active oxygen content, the best test concentration of the active ingredient is 0.1%. The compositions obtained in Example 1, Comparative Example 2 and Comparative Example 5 of the present invention are diluted with water to this concentration for testing.

[0083] Table 6 Cellular reactive oxygen content

[0084] Sample source Active oxygen content / % Example 1 32.76 Comparative Example 2 81.22 Comparative Example 5 75.49 Control group 100

[0085] In terms of inhibiting the active oxygen content in cells, the composition of Example 1 has an excellent antioxidant effect, with the active oxygen content in its cells being 32.76%, the active oxygen content in Comparative Example 2 being 81.22%, and the active oxygen content in Comparative Example 5 being 75.49%. In terms of the inhibitory effect on the active oxygen content, the inhibition rate of Comparative Example 2 plus Comparative Example 5 is 43.29%. Similarly, its inhibition rate is also much lower than 67.24% in Example 1. That is to say, in terms of antioxidant effect, the Pyracantha fortuneana fruit extract and the modified silk-wrapped sunscreen in this case also have obvious synergistic effects and have better antioxidant effects.

[0086] Effect of the test composition on the content of collagen I and IV

[0087] NHDF cells were inoculated into 6-well culture plates with 2 mL of culture medium per well and placed in a 37°C, 5% CO 2 Incubate in an incubator for 12 hours. Then add the test solution and incubate for 72 hours. 2 UVA irradiation was performed for 30 min. After irradiation, the cells were harvested and the contents of collagen I and collagen IV were detected by ELISA. The test results are shown in Table 7.

[0088] According to preliminary tests, in the test of collagen content, the best active ingredient test concentration is 0.1%. The compositions obtained in Example 1, Comparative Example 2 and Comparative Example 5 of the present invention are diluted with water to this concentration for testing.

[0089] Table 7 Collagen Ⅰ and Ⅳ content

[0090]

[0091]

[0092] It can be seen from Table 7 that in terms of promoting collagen synthesis, whether it is collagen I or collagen IV, the composition of Example 1 has an obvious effect of promoting synthesis, and the contents of collagen I and collagen IV are 55.16 and 60.48, respectively, which are 45.16 and 53.48 higher than that of the control group; while Comparative Examples 2 and 5 also have a certain effect of promoting synthesis relative to the control group, but the promotion effect is relatively low. Even if the results of Comparative Examples 2 and 5 are added together, collagen I is only 38.70 and collagen IV is 34.27, which are far less than Example 1, indicating that the Pyracantha fortuneana fruit extract and modified silk-wrapped sunscreen in the composition of Example 1 of this case also have a synergistic effect in promoting collagen synthesis, and can exert a high-efficiency anti-aging effect.

[0093] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

Claims

1. A sunscreen composition, characterized in that: The sunscreen composition comprises the following components: pyracantha fruit extract and modified silk-wrapped sunscreen agent, wherein the mass portion of the pyracantha fruit extract is 20 to 35 parts, and the mass portion of the modified silk-wrapped sunscreen agent is 65 to 80 parts.

2. The sunscreen composition according to claim 1, characterized in that The sunscreen composition further comprises 10 to 15 parts by weight of an encapsulated cooling agent.

3. The sunscreen composition according to claim 2, characterized in that The specific components of the modified silk-wrapped sunscreen include, by mass percentage, 10wt% to 70wt% sunscreen, 5wt% to 10wt% polysiloxane-14, 2wt% butylene glycol, 2wt% phenoxyethanol, 0.2wt% EDTA-2Na, and the balance water.

4. The sunscreen composition according to claim 3, characterized in that The sunscreen is at least one of ethylhexyl methoxycinnamate, octocrylene, butyl methoxydibenzoylmethane, and diethylaminohydroxybenzoyl hexyl benzoate.

5. The sunscreen composition according to claim 2, characterized in that The specific components of the encapsulated cooling agent include, by mass percentage: 2.5wt% to 7wt% menthol lactate, 2.5wt% to 7wt% methyl menthane carboxamide, 2.5wt% to 7wt% methyl diisopropylamide, 2.5wt% to 7wt% hydroxypropyl cellulose, 1wt% to 5wt% isopropyl palmitate, 0.3wt% to 0.75wt% phenoxyethanol, 0.3wt% to 0.75wt% ethylhexylglycerin, and the balance is propylene glycol.

6. Use of the sunscreen composition according to any one of claims 1 to 5 in preparing skin care products.

7. The sunscreen composition according to claim 6, characterized in that The skin care product is a skin care product used for sun protection.

8. A skin care product, characterized in that: The skin care product contains 10wt% to 40wt% of the sunscreen composition according to any one of claims 1 to 5.

9. The skin care product according to claim 8, characterized in that: The skin care products include lotions, creams, sprays, essences, essential oils, cleansers, lotions, facial masks, and gels.

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