Natural component composition with anti-allergy and red-removing effects as well as preparation method and application of natural component composition

Abstract

The invention provides a natural component composition with anti-allergy and red-removing effects as well as a preparation method and application thereof. The natural component composition comprises a paris polyphylla extract, a radix sophorae flavescentis extract, a cactus extract and a Sinkiang lithospermum extract. The invention creatively finds that the paris polyphylla extract, the sophora flavescens extract, the cactus extract and the Sinkiang lithospermum extract are matched for use, have a synergistic effect in the aspects of resisting allergy, relieving and removing redness, not only can reduce the phenomenon of skin redness, but also can inhibit inflammatory factors, and further has a remarkable effect of enhancing the skin barrier.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and specifically relates to a natural ingredient composition with anti-allergic and redness-removing effects, a preparation method and application thereof. Background Art

[0002] Sensitive skin has become the biggest challenge modern people face in skin care. The most obvious manifestation is that the skin becomes itchy and red, causing serious discomfort in work and life.

[0003] The reason why more and more people have sensitive skin is closely related to lifestyle habits, skin care materials and environmental changes, in addition to a small number of congenital factors. From the analysis of raw material properties, most synthetic ingredients have a greater risk of irritation, and long-term use will increase skin sensitivity; from the analysis of lifestyle habits, modern people often stay up late and have irregular sleep, and face electronic devices for a long time; from the analysis of environmental changes, industrialization has caused certain pollution to air and water quality, and long-term contact with unclean or harmful bacteria-contaminated air and water is also one of the causes of sensitive skin.

[0004] Mechanisms causing skin sensitivity and redness: 1. Damage to the skin barrier function: Weak skin barrier function increases the permeability of the stratum corneum, making it easier for external substances to enter the skin and cause irritation. Main manifestations: fragile, thin, dry, peeling skin, and high TEWL index. 2. Inflammatory response intensifies: External irritants penetrate, and the skin releases a large amount of inflammatory mediators, leading to vasodilation, increased permeability, and typical symptoms of redness, swelling, heat, and pain. Main manifestations: Skin inflammation and redness. 3. High reactivity of nerve endings: Inflammatory agents are released after inflammation and tissue damage to stimulate primary sensory neurons. Skin sensitivity and neurogenic inflammation (more susceptible to pain signals mediated by inflammatory factors). Main manifestations: itching and tingling of the skin.

[0005] From the above analysis of the causes of skin sensitivity and redness, the way to solve the problem of sensitive skin is to improve anti-allergy, anti-inflammatory and repair capabilities. Skin care products made with natural ingredients as raw materials are expected to completely and permanently solve this problem and are also the most popular among consumers. my country is the country with the longest history of applying herbal medicines, and at the same time has advanced technology in the field of natural ingredient extraction, which provides a solid foundation for the preparation of natural skin care raw materials.

[0006] Plants contain a wide variety of active ingredients such as glycosides, polysaccharides, polyphenols, alkaloids, etc. Some of these raw materials have been used in various skin care products, but there is still a lack of products with significant efficacy in the field of skin allergy and redness removal. Summary of the invention

[0007] In view of the deficiencies of the prior art, the present invention provides a natural ingredient composition with anti-allergic and anti-redness effects, and a preparation method and application thereof. The composition is a pure natural plant composition, which is used to solve the problem of anti-allergic and anti-redness of sensitive skin and can be applied on a large scale.

[0008] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0009] In a first aspect, the present invention provides a natural ingredient composition with anti-allergic and redness-removing effects, wherein the natural ingredient composition comprises a seven-leaf parsley extract, a sophora flavescens extract, a cactus extract and a lithospermum officinale extract.

[0010] The extract of Parsley in the composition of the present invention contains total saponins I, II and VII of Paris polyphylla, which have significant analgesic and sedative effects; the extract of Sophora flavescens contains matrine and oxymatrine, which have inhibitory effects on Escherichia coli, Staphylococcus aureus and Candida albicans; the extract of Lithospermum erythrorhizon has both anti-inflammatory and detumescent effects and can also be used as a natural pigment; and the cactus extract is a polysaccharide substance, which has a significant effect on hyaluronidase inhibition and has obvious anti-allergic activity. The present invention creatively discovered that the combination of the four has a synergistic effect in anti-allergic, soothing and removing redness, which can not only reduce skin redness, but also inhibit inflammatory factors, thereby significantly enhancing the skin barrier.

[0011] Preferably, the natural ingredient composition comprises, by weight, 1-30 parts of Paridis aestivus extract, 1-20 parts of Sophora flavescens extract, 1-10 parts of Opuntia dioica extract and 1-30 parts of Lithospermum officinale extract.

[0012] The amount of the extract of the seven-leaf gentiana extract in the natural ingredient composition with anti-allergic and redness-removing effects of the present invention is 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 17 parts, 19 parts, 20 parts, 21 parts, 23 parts, 25 parts, 27 parts or 29 parts, etc.;

[0013] The added amount of the Sophora flavescens extract is 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 17 parts or 19 parts, etc.;

[0014] The added amount of the cactus extract can be 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts or 9 parts, etc.;

[0015] The added amount of the Xinjiang lithospermum extract is 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, 17 parts, 19 parts, 20 parts, 21 parts, 23 parts, 25 parts, 27 parts or 29 parts, etc.

[0016] Preferably, the natural ingredient composition comprises, by weight, 5-25 parts of Paridis aestivus extract, 5-18 parts of Sophora flavescens extract, 2-8 parts of Opuntia dioica extract and 5-25 parts of Lithospermum officinale extract.

[0017] Preferably, the natural ingredient composition also includes Ophiopogon japonicus extract.

[0018] The Ophiopogon japonicus extract has a strong ability to scavenge hydroxyl free radicals, and has a certain inhibitory effect on Gram-negative bacteria Escherichia coli and fungus Candida. By adding the Ophiopogon japonicus extract to the composition of the present invention, the anti-allergic, soothing and redness-removing effects of the composition can be further improved.

[0019] Preferably, the mass ratio of the Herba Lysimachiae extract to the Herba Ophiopogonis extract is 1:(0.1-1), for example, it may be 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7, 1:0.8 or 1:0.9, etc.

[0020] Preferably, the mass ratio of the Herba Lysimachiae extract to the Herba Ophiopogonis extract is 1:(0.2-0.5).

[0021] Preferably, the Aesculus multiflorus extract, Sophora flavescens extract or Lithospermum erythrorhizon extract is prepared by the following method: crushing Aesculus multiflorus, Sophora flavescens or Lithospermum erythrorhizon and adding them to polyol, extracting under ultrasound, filtering the extract and taking the clear liquid to obtain the Aesculus multiflorus extract, Sophora flavescens extract or Lithospermum erythrorhizon extract.

[0022] The preparation method of the Sophora flavescens extract or the Xinjiang Lithospermum officinale extract is the same as the preparation method of the Seven-leaved Parsley extract except for the different raw materials, and the other steps are the same, and the process parameters are independently selected within the following ranges.

[0023] Preferably, the Herba Polygoni Multiflori, Sophorae Flavescentis or Lithospermum Officinale are independently crushed to 50-80 mesh, for example, 52 mesh, 55 mesh, 58 mesh, 60 mesh, 62 mesh, 65 mesh or 68 mesh.

[0024] Preferably, the mass ratio of the seven-leaf parsley, sophora flavescens or lithospermum officinale to the polyol is independently 1:(4-7), for example, it can be 1:4.2, 1:4.5, 1:4.8, 1:5, 1:5.3, 1:5.5, 1:5.8, 1:6, 1:6.2, 1:6.5 or 1:6.8, etc.

[0025] Preferably, the polyols each independently include any one of glycerol, 1,2-hexanediol, butanediol or 1,2-pentanediol, or a combination of at least two thereof.

[0026] Preferably, the polyols are glycerol and 1,2-hexanediol.

[0027] Preferably, the mass ratio of glycerol to 1,2-hexanediol is 1:(0.1-0.5), for example, it can be 1:0.1.5, 1:0.2, 1:0.25, 1:0.3, 1:0.35, 1:0.4 or 1:0.45, etc.

[0028] Preferably, the extraction temperature is independently 40-50°C (for example, 41°C, 43°C, 45°C, 47°C or 49°C, etc.), and the extraction time is independently 40-80min (for example, 45min, 50min, 55min, 60min, 65min, 70min or 75min, etc.).

[0029] Preferably, the frequencies of the ultrasound are each independently 30-50 KHz, for example, 31 KHz, 33 KHz, 35 KHz, 37 KHz, 40 KHz, 42 KHz, 45 KHz, 47 KHz or 49 KHz, etc.

[0030] Preferably, the particle size of each filter element used for the filtration is independently 0.15-0.3 μm, for example, it can be 0.18 μm, 0.2 μm, 0.22 μm, 0.25 μm, 0.27 μm or 0.29 μm, etc.

[0031] Preferably, the cactus extract is prepared by the following method: crushing the cactus and mixing it with water, extracting it under heating, filtering the extract and taking the clear liquid as the cactus extract.

[0032] Preferably, the crushing is to 50-80 mesh, for example, 52 mesh, 55 mesh, 58 mesh, 60 mesh, 62 mesh, 65 mesh or 68 mesh.

[0033] Preferably, the mass ratio of the cactus to water is 1:(5-6), for example, it can be 1:5.1, 1:5.3, 1:5.5, 1:5.7 or 1:5.9.

[0034] Preferably, the heating is to 90-120°C, for example, 92°C, 95°C, 98°C, 100°C, 102°C, 105°C, 108°C, 110°C, 112°C, 115°C or 118°C, etc.

[0035] Preferably, the extraction time is 1.5-3 h, for example, it can be 1.6 h, 1.8 h, 2 h, 2.2 h, 2.5 h, 2.7 h or 2.9 h.

[0036] Preferably, the particle size of the filter element used for the filtration is 0.15-0.3 μm, for example, it can be 0.18 μm, 0.2 μm, 0.22 μm, 0.25 μm, 0.27 μm or 0.29 μm, etc.

[0037] The preparation method of the Ophiopogon japonicus extract and the preparation method of the cactus extract are the same in steps except for the different raw materials, and the process parameters are independently selected within the following ranges.

[0038] Preferably, the Ophiopogon japonicus extract is prepared by the following method: crushing the Ophiopogon japonicus and mixing it with water, extracting it under heating, filtering the extract and taking the clear liquid as the cactus extract.

[0039] Preferably, the crushing is to 50-80 mesh, for example, 52 mesh, 55 mesh, 58 mesh, 60 mesh, 62 mesh, 65 mesh or 68 mesh.

[0040] Preferably, the mass ratio of the Ophiopogon japonicus to water is 1:(5-6), for example, it can be 1:5.1, 1:5.3, 1:5.5, 1:5.7 or 1:5.9, etc.

[0041] Preferably, the heating is to 90-120°C, for example, 92°C, 95°C, 98°C, 100°C, 102°C, 105°C, 108°C, 110°C, 112°C, 115°C or 118°C, etc.

[0042] Preferably, the extraction time is 1.5-3 h, for example, it can be 1.6 h, 1.8 h, 2 h, 2.2 h, 2.5 h, 2.7 h or 2.9 h.

[0043] Preferably, the particle size of the filter element used for the filtration is 0.15-0.3 μm, for example, it can be 0.18 μm, 0.2 μm, 0.22 μm, 0.25 μm, 0.27 μm or 0.29 μm, etc.

[0044] Preferably, the total content of paris saponin I, paris saponin II and paris saponin VII in the seven-leaf parsley extract of the present invention is ≥0.05%, the total content of matrine and oxymatrine in the sophora flavescens extract is ≥0.2%; the total polysaccharide content in the cactus extract is ≥1%; the β,β'-dimethylacryloyl akanin content of the lithospermum erythrorhizon extract is ≥0.03%; and the total polysaccharide content of the ophiopogon japonicus extract is ≥5%.

[0045] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0046] In a second aspect, the present invention provides a method for preparing a natural ingredient composition with anti-allergic and redness-removing effects as described in the first aspect, the preparation method comprising: mixing a seven-leaf parsley extract, a sophora flavescens extract, a cactus extract and a lithospermum officinale extract, and fusing them through high-pressure microjetting to obtain the natural ingredient composition.

[0047] Preferably, the mixing further comprises adding Ophiopogon japonicus extract.

[0048] Preferably, the process conditions of the high-pressure microfluidization are: temperature of 30-45°C (for example, 31°C, 33°C, 35°C, 37°C, 40°C, 42°C or 44°C, etc.), pressure of 18000-25000PSI (for example, 19000PSI, 20000PSI, 21000PSI, 22000PSI, 23000PSI or 24000PSI, etc.).

[0049] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0050] In a third aspect, the present invention provides a use of the natural ingredient composition with anti-allergic and anti-redness effects as described in the first aspect in the preparation of cosmetics.

[0051] In a fourth aspect, the present invention provides a cosmetic, wherein the raw material composition of the cosmetic comprises the natural ingredient composition with anti-allergic and redness-removing effects as described in the first aspect.

[0052] Preferably, the cosmetic is a lotion, a serum or a facial mask.

[0053] Preferably, the amount of the natural ingredient composition with anti-allergic and anti-redness effects added to cosmetics is 0.1-20% (for example, 0.1%, 0.5%, 1%, 2%, 5%, 8%, 10%, 15%, 17% or 19%, etc.), preferably 1-10%.

[0054] Preferably, the raw material composition of the cosmetic further includes additives, and the additives include any one or a combination of at least two of solvents, emulsifiers, moisturizers, preservatives or thickeners.

[0055] Any specific point value within the above numerical range can be selected, and will not be described in detail here.

[0056] Compared with the prior art, the present invention has the following beneficial effects:

[0057] (1) The present invention creatively discovered that the combination of Aesculus quinquefolius extract, Sophora flavescens extract, Opuntia cactus extract and Lithospermum officinale extract has a synergistic effect in anti-allergic, soothing and removing redness, which can not only reduce skin redness, but also inhibit inflammatory factors, thereby significantly enhancing the skin barrier.

[0058] (2) The present invention specifies different extraction methods according to the performance of different components, which can not only better extract the active ingredients of each component, but also avoid the oxidation or hydrolysis of each active ingredient through different process conditions. DETAILED DESCRIPTION

[0059] The technical solution of the present invention is further described below by specific implementation methods. It should be understood by those skilled in the art that the embodiments are only to help understand the present invention and should not be regarded as specific limitations of the present invention.

[0060] Preparation Example 1.1

[0061] This preparation example provides a Herba Lycopodii Extract, and the preparation method of the Herba Lycopodii Extract is as follows:

[0062] Take 200g of dried Polygonum multiflorum and crush it into 60 mesh, put it into an ultrasonic extractor, add 1kg of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.3), extract at 45℃ and 40KHz for 1 hour, and filter with a 0.2μm PP filter to obtain the clear liquid, which is the Polygonum multiflorum extract.

[0063] Preparation Example 1.2

[0064] This preparation example provides a Herba Lycopodii Extract, and the preparation method of the Herba Lycopodii Extract is as follows:

[0065] Take 200g of dried Polygonum multiflorum and crush it into 50 mesh, put it into an ultrasonic extractor, add 800g of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.5), extract for 70min at 40℃ and 45KHz ultrasonic frequency, and filter with a 0.18μm PP filter to obtain the clear liquid, which is the Polygonum multiflorum extract.

[0066] Preparation Example 1.3

[0067] This preparation example provides a Herba Lycopodii Extract, and the preparation method of the Herba Lycopodii Extract is as follows:

[0068] Take 200g of dried Polygonum multiflorum and crush it into 70 mesh, put it into an ultrasonic extractor, add 1.2kg of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.4), extract for 50min at 50℃ and 35KHz ultrasonic frequency, and filter with a 0.28μm PP filter to obtain the clear liquid, which is the Polygonum multiflorum extract.

[0069] Preparation Example 1.4

[0070] This preparation example provides a Herba Lycopodii Extract, the preparation method of which is different from that of Preparation Example 1.1 in that the polyol is replaced with an equal amount of water, and the remaining steps and process parameters are consistent with those of Preparation Example 1.1.

[0071] Preparation Example 1.5

[0072] This preparation example provides a seven-leaf smilax china extract. The preparation method of the seven-leaf smilax china extract is different from that of Preparation Example 1.1 only in that the polyol is only propylene glycol and the amount of the polyol is kept unchanged. The remaining steps and process parameters are consistent with those of Preparation Example 1.1.

[0073] Preparation Example 1.6

[0074] This preparation example provides a seven-leaf smilax china extract. The preparation method of the seven-leaf smilax china extract is different from that of Preparation Example 1.1 only in that the polyol is only 1,2-hexanediol and the amount of the polyol is kept unchanged. The other steps and process parameters are consistent with those of Preparation Example 1.1.

[0075] Preparation Example 2.1

[0076] This preparation example provides a Xinjiang lithospermum extract, and the preparation method of the Xinjiang lithospermum extract is:

[0077] Take 200g of dried Xinjiang lithospermum officinale, crush it into 50 mesh, put it into an ultrasonic extractor, add 800g of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.5), extract for 70min at 40℃ and 45KHz ultrasonic frequency, and filter with a 0.2μm PP filter to obtain the clear liquid, which is the Xinjiang lithospermum officinale extract.

[0078] Preparation Example 2.2

[0079] This preparation example provides a Xinjiang lithospermum extract, and the preparation method of the Xinjiang lithospermum extract is:

[0080] Take 200g of dried Xinjiang lithospermum officinale, grind it into 50 mesh, put it into an ultrasonic extractor, add 800g of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.4), extract for 70min at 40℃ and 45KHz ultrasonic frequency, and filter with a 0.25μm PP filter to obtain the clear liquid, which is the Xinjiang lithospermum officinale extract.

[0081] Preparation Example 2.3

[0082] This preparation example provides a Xinjiang lithospermum extract, and the preparation method of the Xinjiang lithospermum extract is:

[0083] Take 200g of dried Xinjiang lithospermum, grind it into 70 mesh, put it into an ultrasonic extractor, add 1.2kg of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.3), extract at 50℃ and 35KHz for 50min, and filter with a 0.3μm PP filter to obtain a clear liquid, which is the Xinjiang lithospermum extract.

[0084] Preparation Example 2.4

[0085] This preparation example provides a Xinjiang lithospermum extract. The preparation method of the Xinjiang lithospermum extract is different from that of Preparation Example 2.1 only in that the polyol is replaced by an equal amount of water, and the remaining steps and process parameters are consistent with those of Preparation Example 2.1.

[0086] Preparation Example 3.1

[0087] This preparation example provides a Sophora flavescens extract, and the preparation method of the Sophora flavescens extract is as follows:

[0088] Take 200g of dried Sophora flavescens, grind it into 70 mesh, put it into an ultrasonic extractor, add 1.2kg of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.4), extract at 50℃ and 35KHz for 50min, and filter with a 0.2μm PP filter to obtain the clear liquid, which is the Sophora flavescens extract.

[0089] Preparation Example 3.2

[0090] This preparation example provides a Sophora flavescens extract, and the preparation method of the Sophora flavescens extract is as follows:

[0091] Take 200g of dried Sophora flavescens and crush it into 50 mesh, put it into an ultrasonic extractor, add 800g of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.5), extract at 40℃ and 45KHz for 70min, and filter with a 0.3μm PP filter to obtain the clear liquid, which is the Sophora flavescens extract.

[0092] Preparation Example 3.3

[0093] This preparation example provides a Xinjiang lithospermum extract, and the preparation method of the Xinjiang lithospermum extract is:

[0094] Take 200g of dried Xinjiang lithospermum, grind it into 70 mesh, put it into an ultrasonic extractor, add 1.2kg of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.3), extract at 50℃ and 35KHz for 50min, and filter with a 0.15μm PP filter to obtain a clear liquid, which is the Xinjiang lithospermum extract.

[0095] Preparation Example 3.4

[0096] This preparation example provides a Sophora flavescens extract, and the preparation method of the Sophora flavescens extract is different from that of Preparation Example 3.1 only in that the polyol is replaced by an equal amount of water, and the remaining steps and process parameters are consistent with those of Preparation Example 3.1.

[0097] Preparation Example 4.1

[0098] This preparation example provides a cactus extract, and the preparation method of the cactus extract is as follows:

[0099] Take 200g of dried cactus and crush it into 60 mesh, put it into a heating extraction tank, add 1kg of pure water, extract at 100℃ for 2 hours, and filter with a 0.2μm PP filter to obtain the clear liquid, which is the cactus extract.

[0100] Preparation Example 4.2

[0101] This preparation example provides a cactus extract, and the preparation method of the cactus extract is as follows:

[0102] Take 200g of dried cactus and crush it into 50 mesh, put it into a heating extraction tank, add 1.1kg of pure water, extract at 90℃ for 3 hours, and filter with a 0.25μm PP filter to obtain the clear liquid, which is the cactus extract.

[0103] Preparation Example 4.3

[0104] This preparation example provides a cactus extract, and the preparation method of the cactus extract is as follows:

[0105] Take 200g of dried cactus and crush it into 80 mesh, put it into a heating extraction tank, add 1.2kg of pure water, extract at 120℃ for 1.5 hours, and filter with a 0.15μm PP filter to obtain the clear liquid, which is the cactus extract.

[0106] Preparation Example 4.4

[0107] This preparation example provides a cactus extract. The preparation method of the cactus extract differs from that of Preparation Example 4.1 only in that water is replaced with an equal amount of polyol (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.5), and the remaining steps and process parameters are consistent with those of Preparation Example 4.1.

[0108] Preparation Example 5.1

[0109] This preparation example provides a Radix Ophiopogonis extract, and the preparation method of the Radix Ophiopogonis extract is as follows:

[0110] Take 200g of dried Ophiopogon japonicus, grind it into 50 mesh, put it into a heating extraction tank, add 1.2kg of pure water, extract at 110℃ for 2.5 hours, and filter with a 0.2μm PP filter to obtain the clear liquid, which is the Ophiopogon japonicus extract.

[0111] Preparation Example 5.2

[0112] This preparation example provides a Radix Ophiopogonis extract, and the preparation method of the Radix Ophiopogonis extract is as follows:

[0113] Take 200g of dried Ophiopogon japonicus, grind it into 50 mesh, put it into a heating extraction tank, add 1.1kg of pure water, extract at 90℃ for 3 hours, and filter with a 0.3μm PP filter to obtain the clear liquid, which is the Ophiopogon japonicus extract.

[0114] Preparation Example 5.3

[0115] This preparation example provides a Radix Ophiopogonis extract, and the preparation method of the Radix Ophiopogonis extract is as follows:

[0116] Take 200g of dried Ophiopogon japonicus, grind it into 70 mesh, put it into a heating extraction tank, add 1.2kg of pure water, extract at 120℃ for 2.5 hours, and filter with a 0.15μm PP filter to obtain the clear liquid, which is the Ophiopogon japonicus extract.

[0117] Preparation Example 5.4

[0118] This preparation example provides an Ophiopogon japonicus extract, the preparation method of which is different from that of Preparation Example 5.1 in that water is replaced with an equal amount of polyols (propylene glycol and 1,2-hexanediol in a mass ratio of 1:0.5), and the remaining steps and process parameters are consistent with those of Preparation Example 5.1.

[0119] Examples 1-8

[0120] This embodiment provides eight natural ingredient compositions with anti-allergic and anti-redness effects, and the components of the natural ingredient compositions are shown in Tables 1.1-1.2 below:

[0121] Table 1.1

[0122]

[0123] Table 1.2

[0124]

[0125]

[0126] The preparation method of the natural ingredient composition with anti-allergic and redness-removing effects described in Example 1 is as follows:

[0127] The extract of Aesculus multiflorus, Sophora flavescens extract, Opuntia cactus extract, Ophiopogon japonicus extract and Lithospermum officinale extract are mixed in proportion, then heated to 40°C and fused by high-pressure microfluidization (pressure of 20,000 PSI) to obtain the natural ingredient composition with anti-allergic and redness-removing effects.

[0128] The preparation methods of Examples 2-8 refer to Example 1.

[0129] Comparative Example 1

[0130] This comparative example provides a natural ingredient composition with anti-allergic and redness-removing effects. The only difference between the composition and Example 1 is that the composition does not contain the seven-leaf flos spatholobi extract, and the reduced amount is distributed to the sophora flavescens extract, the cactus extract and the lithospermum officinale extract in proportion to the number of parts. The other components and amounts remain unchanged, and the preparation method refers to Example 1.

[0131] Comparative Example 2

[0132] This comparative example provides a natural ingredient composition with anti-allergic and redness-removing effects. The only difference between the composition and Example 1 is that the Sophora flavescens extract is not included in the components, and the reduced amount is distributed to the Seven-leaf Parsley extract, the cactus extract and the Xinjiang Lithospermum officinale extract in proportion to the number of parts. The other components and amounts remain unchanged, and the preparation method refers to Example 1.

[0133] Comparative Example 3

[0134] This comparative example provides a natural ingredient composition with anti-allergic and redness-removing effects. The only difference between the composition and Example 1 is that the cactus extract is not included in the components, and the reduced amount is distributed to the seven-leaf parsnip extract, sophora flavescens extract and Xinjiang lithospermum extract according to the proportion of parts. The other components and amounts remain unchanged, and the preparation method refers to Example 1.

[0135] Comparative Example 4

[0136] This comparative example provides a natural ingredient composition with anti-allergic and redness-removing effects. The only difference between the composition and Example 1 is that the Xinjiang Lithospermum officinale extract is not included in the components, and the reduced amount is distributed to the Seven-leaf Parsley Extract, Sophora flavescens Extract and Cactus Extract in proportion to the number of parts. The other components and amounts remain unchanged, and the preparation method refers to Example 1.

[0137] Application Examples 1-3

[0138] This application example provides three kinds of essences with anti-allergic and anti-redness effects. The components of the essences are shown in Table 2 below. The dosage of each component in the table is in parts:

[0139] Table 2

[0140]

[0141] The preparation method of the essence with anti-allergic and anti-redness effects is as follows: water, a moisturizer, and a thickener are added into a reaction kettle, heated to 80° C., mixed and dispersed, and then an emulsifier is added for mixing and dispersion, cooled to 55° C., and a fragrance and a natural ingredient composition with anti-allergic and anti-redness effects are added, and the essence is obtained after uniform dispersion.

[0142] Application Examples 4-8

[0143] The application example provides five essences with anti-allergic and redness-removing effects. The only difference between the application example and application example 1 is that the natural ingredient composition with anti-allergic and redness-removing effects in Example 1 is replaced by the natural ingredient composition with anti-allergic and redness-removing effects in Examples 4-8 in equal amounts, and the other components and amounts remain unchanged. The preparation method refers to that in Application Example 1.

[0144] Comparative Application Examples 1-4

[0145] The comparative application example provides four essences with anti-allergic and redness-removing effects. The only difference between them and Application Example 1 is that the natural ingredient composition with anti-allergic and redness-removing effects of Example 1 is replaced by the natural ingredient composition with anti-allergic and redness-removing effects of Comparative Examples 1-4 in equal amounts, and the other components and amounts remain unchanged. The preparation method refers to Application Example 1.

[0146] Comparative Application Example 5

[0147] The comparative application example provides an essence with anti-allergic and anti-redness effects, which differs from Application Example 1 only in that it does not include the natural ingredient composition with anti-allergic and anti-redness effects of Example 1, and the other components and amounts remain unchanged. The preparation method refers to Application Example 1.

[0148] Test Example 1: Active ingredient detection

[0149] The total contents of Paris polyphylla saponins I, II and VII were determined in the Paridis chinensis extracts obtained in Preparation Examples 1.1 and 1.4-1.6; the β,β'-dimethylacryloyl akanin content of the Lithospermum officinale extracts obtained in Preparation Examples 2.1 and 2.4 was determined; the total contents of matrine and oxymatrine in the Sophora flavescens extracts obtained in Preparation Examples 3.1 and 3.4 were determined; the total content of total polysaccharides in the cactus extracts obtained in Preparation Examples 4.1 and 4.4 was determined; and the total polysaccharide content in the Ophiopogon japonicus extracts obtained in Preparation Examples 5.1 and 5.4 was determined.

[0150] The total polysaccharide content is determined by sulfuric acid-phenol colorimetry and ultraviolet spectrophotometry.

[0151] Test method:

[0152] (1) Preparation of reference solution: Accurately weigh 10 mg of anhydrous glucose dried to constant weight at 120°C and place it in a 100 ml volumetric flask. Add pure water to the mark and shake well to prepare a 0.1 mg / mL reference solution.

[0153] (2) Preparation of standard curve: Prepare glucose standard solutions with concentrations of 0.02, 0.04, 0.06, 0.08, and 0.10 mg / mL. Accurately pipette 1 mL of the standard solution into a test tube, add 1 mL of a 5% phenol solution, then add 5 mL of concentrated sulfuric acid, shake well, place in a boiling water bath for 15 min, cool to room temperature, and measure the absorbance at a wavelength of 485 nm. Draw a standard curve with concentration as the horizontal axis and absorbance as the vertical axis. The result requires R2 ≥ 0.999.

[0154] (3) Determination method: Take 1 mL of the filtered extract, add 1 mL of 5% phenol solution (phenol must be prepared before use), then add 5 mL of concentrated sulfuric acid to the test tube, boil in water for 15 min, cool to room temperature, and measure with a UV spectrophotometer.

[0155] The content of total saponins I, II, and VII of Paris polyphylla is determined according to the high performance liquid chromatography method (General Rule 0512) of the 2020 edition of the Chinese Pharmacopoeia;

[0156] (1) Chromatographic conditions: octadecylsilane bonded silica gel as filler; acetonitrile as mobile phase A, water as mobile phase B, gradient elution as specified in the table below; detection wavelength is 203 nm. The theoretical plate number calculated based on the Paris polyphylla saponin I peak should be no less than 4000.

[0157] Time (minutes) Mobile phase A (%) Mobile phase B (%) 0-40 30-60 70-40 40-50 60-30 40-70

[0158] (2) Preparation of reference solution: Take appropriate amounts of Paris saponin I reference substance, Paris saponin II reference substance and Paris saponin VII reference substance, weigh them accurately, and add methanol to prepare a mixed solution containing 0.4 mg of each reference substance per ml.

[0159] (3) Determination method: Accurately pipette 10 μl of reference solution and extract solution respectively, inject into liquid chromatograph, and determine.

[0160] The contents of matrine and oxymatrine are determined according to the high performance liquid chromatography method (General Rule 0512) of the 2020 edition of the Chinese Pharmacopoeia.

[0161] (1) Chromatographic conditions: Octadecylsilane bonded silica gel as filler; acetonitrile-[0.01 mol / L ammonium acetate solution (adjusted to pH 8.1 with concentrated ammonia test solution)] (3:2) as mobile phase A, 0.01 mol / L ammonium acetate solution (adjusted to pH 8.1 with concentrated ammonia test solution) as mobile phase B, gradient elution as specified in the following table; detection wavelength of 225 nm, theoretical plate number calculated based on the oxymatrine peak should be no less than 4000.

[0162]

[0163] (2) Preparation of reference solution: Take appropriate amount of matrine reference substance and oxymatrine reference substance, weigh accurately, add ethanol to prepare solutions containing 50 μg of matrine and 0.15 mg of oxymatrine per 1 ml.

[0164] (3) Determination method: Accurately pipette 5 μl of each of the two reference solutions and 5-10 μl of the extract, inject into liquid chromatograph, and determine.

[0165] The β,β'-dimethylacryloyl akanin content is determined according to the high performance liquid chromatography method (General Rule 0512) of the 2020 edition of the Chinese Pharmacopoeia.

[0166] (1) Chromatographic conditions: Octadecylsilane bonded silica gel as filler; acetonitrile-water-formic acid (70:30:0.05) as mobile phase; detection wavelength at 275 nm. The theoretical plate number calculated based on the β,β'-dimethylacryloyl akanin peak should be no less than 2000.

[0167] (2) Preparation of reference solution: Take an appropriate amount of β,β'-dimethylacryloyl akanin reference substance, accurately weigh it, and add ethanol to make a solution containing 0.1 mg per 1 ml.

[0168] (3) Determination method: Accurately pipette 10 μl of reference solution and extract solution respectively, inject into liquid chromatograph, and determine.

[0169] The results are shown in Table 3.

[0170] Table 3

[0171]

[0172] From the results in Table 3, it can be seen that the content of active ingredients in the composition of the present invention is higher only when the extraction method of the Herba Lysimachiae extract, Sophorae Flavescentis extract, Opuntia cactus extract, Lithospermum officinale extract and Ophiopogon japonicus extract is extracted by the method defined in the present invention, and the selection of polyols also has a certain influence on the extraction of the active ingredient content.

[0173] Test Example 2: Sodium hyaluronate inhibition rate test

[0174] Hyaluronidase inhibition rate. The higher the hyaluronidase inhibition rate, the better the soothing effect.

[0175] Test objects: compositions obtained from Examples 1-8 and Comparative Examples 1-4.

[0176] The test method is as follows:

[0177] Hyaluronidase is a specific cleavage enzyme of hyaluronic acid and is often used to evaluate in vitro anti-allergic activity. Hyaluronic acid plays a major inhibitory role in the development and regulation of the human body (such as cell adhesion, organ formation, wound healing, tumorigenesis, and angiogenesis). Inhibiting the activity of hyaluronidase can prevent sodium hyaluronate from being decomposed and maintain normal physiological functions. Therefore, the hyaluronidase inhibition rate is used as the anti-allergic standard. The specific grading standards are shown in Table 4.

[0178] Table 4

[0179] Inhibition rate Grading ≧70％ Strong anti-allergic effect ≧50％ Moderate anti-allergic effect [30％,50％] Weak anti-allergic effect ﹤30％ No anti-allergic effect

[0180] 0.1 mL of 0.25 mmol / L CaCL2 solution, 0.5 mL of 500 U / mL hyaluronidase solution and an appropriate amount of acetate buffer (pH = 5.6) were respectively transferred into 20 conical flasks, gently shaken and mixed, and placed in a 37 ° C constant temperature incubator for 20 min, and then 0.5 mL of deionized water and 0.5 mL of sample solution (prepared by the composition obtained in Example 1-8 and Comparative Example 1-4, concentration: 50 mg / mL) were added in sequence, and placed at 37 ° C for 20 min, and then 0.5 mL of 0.5 mg / mL hyaluronidase potassium solution and an appropriate amount of acetate buffer (pH = 5.6) were added, and placed at 37 ° C for 40 min, and then placed at room temperature for 10 min, 0.5 mL of deionized water, 1 drop of 5 mol / L NaOH solution and 1 mL of acetylacetone solution were added, and placed in a boiling water bath for 15 min, immediately cooled with ice water for 5 min, placed at room temperature for 10 min, and then 1 mL was added. P-DAB color developer and an appropriate amount of anhydrous ethanol were placed at room temperature for 30 minutes for color development, and then the absorbance of the sample at 530 nm was measured using a spectrophotometer. The inhibition rate of each group of samples on hyaluronidase was calculated, and a 5% mass fraction of dipotassium glycyrrhizinate solution was used as a positive control. The calculation formula is shown in (1-1), and the calculation results are shown in Table 5.

[0181]

[0182] Where: I is the hyaluronidase inhibition rate;

[0183] A1 is the absorbance value of the (hyaluronidase + sample + hyaluronidase potassium) solution;

[0184] A2 is the absorbance value of the (acetate buffer + sample + acetate buffer) solution;

[0185] A3 is the absorbance value of the (hyaluronidase + deionized water + hyaluronidase potassium) solution;

[0186] A4 is the absorbance value of the (acetic acid buffer + deionized water) solution.

[0187] The results are shown in Table 5.

[0188] Table 5

[0189]

[0190]

[0191] Test Example 3: Skin hemoglobin content test

[0192] The greater the hemoglobin decrease rate, the better the redness-removing effect.

[0193] Test objects: compositions obtained from Examples 1-8 and Comparative Examples 1-4.

[0194] The test method is as follows:

[0195] After the redness model was established on the forearm of the subject by preparing a 1% histamine solution, the skin hemoglobin value of the test area before and after the use of the composition was measured using each composition, T0 is the hemoglobin value when the modeling medication is less than 1 minute, T20 is the hemoglobin value when the modeling medication is 20 minutes, T40 is the hemoglobin value when the modeling medication is 40 minutes, T60 is the hemoglobin value when the modeling medication is 60 minutes, and T blank is the hemoglobin value before modeling. The skin melanin and hemoglobin tester is (MexameterMX18, CK, Germany), and the hemoglobin value at each time point is recorded.

[0196] Hemoglobin decrease rate (%) = [1-(T60-Tblank) ÷ (T0-Tblank)] × 100.

[0197] The results are shown in Table 6.

[0198] Table 6

[0199]

[0200]

[0201] It can be seen from Tables 5 and 6 that the hyaluronidase inhibition rate of the natural ingredient composition with anti-allergic and anti-redness efficacy prepared in Examples 1-3 of the present invention can reach more than 70%, and the hemoglobin reduction rate is more than 70%, indicating that the composition has very good anti-allergic, soothing and anti-redness effects.

[0202] The hyaluronidase inhibition rate in Example 4 was reduced, indicating that adding Ophiopogon japonicus extract to the composition can further improve the anti-allergic, soothing and redness-removing effects.

[0203] It can be seen from Examples 5 and 6 that when the dosage of Ophiopogon japonicus extract is too high, the anti-allergic, soothing and redness-removing effects of the composition cannot be significantly improved, while when the dosage is too low, the anti-allergic and redness-removing effects of the composition will decrease. This shows that only when the dosage of Ophiopogon japonicus extract is within the specified range of the present invention can the performance of the composition be better improved without increasing the cost.

[0204] It can be seen from Examples 7 and 8 that the product with better performance can only be obtained when the extraction method of the Aesculus chinensis extract, Sophora flavescens extract, Opuntia cochinchinensis extract, Lithospermum officinale extract and Ophiopogon japonicus extract in the composition of the present invention is the method defined in the present invention.

[0205] It can be seen from Comparative Examples 1-4 that the Aesculus quinquefolius extract, Sophora flavescens extract, Opuntia cactus extract and Lithospermum officinale extract in the composition of the present invention have a synergistic effect, and the lack of any one of the four will reduce the anti-allergic, soothing and redness-removing properties of the composition to a certain extent.

[0206] Test Example 4: Crowd Trial Evaluation Test:

[0207] Test object: Soothing test was conducted on the essence prepared from Example 1-8 and Comparative Application Example 1-5:

[0208] 78 volunteers aged 20 to 45 were randomly selected as research subjects, including 39 males and 39 females. The tested parts had no skin problems in the past month and no history of systemic diseases. The volunteers were randomly divided into 13 groups, 6 in each group, half male and half female. The subjects were not allowed to wear makeup or use skin care products on the morning of the test day. After cleaning the facial skin with clean water, wait for 30 minutes in a constant temperature and humidity room. Then, 10% lactic acid was applied to the nasolabial groove on either side of the volunteers at room temperature. After 5 minutes of application, the essences of application examples 1-8 and comparative application examples 1-4 were used respectively. After 30 minutes of use, the volunteers were asked to evaluate and score their feelings of use. A 5-point system was used: 4.5 points and above, indicating very good and very satisfied; 4-4.5 points are good; 3-4 points are moderately acceptable; and below 3 points are not good and unacceptable. The scores of each group are the average of 6 people, and the results are shown in Table 7.

[0209] Table 7

[0210]

[0211]

[0212] It can be seen from Table 7 that, compared with Comparative Application Example 5, the essence prepared in Application Examples 1-3 can quickly regulate and relieve skin discomfort symptoms, has excellent immediate soothing effect, and the volunteers have a good experience after use, indicating that the composition provided by the present invention has a rapid soothing effect. By comparing the products of Application Examples 1-4, it can be seen that the combination of the Herba Lycopodii extract, the Herba Sophorae Flavescentis extract, the Herba Opuntiae extract and the Herba Lithospermi sibiricum extract in the present invention has a more significant synergistic effect in soothing and removing redness.

[0213] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate a natural ingredient composition with anti-allergic and redness-removing effects and its preparation method and application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of the raw materials of the product of the present invention, addition of auxiliary ingredients, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0214] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0215] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A natural ingredient composition with anti-allergic and redness-removing effects, characterized in that: The natural ingredient composition comprises a seven-leaf parsley extract, a sophora flavescens extract, a cactus extract and a Xinjiang lithospermum officinale extract.

2. The natural ingredient composition according to claim 1, wherein The natural ingredient composition comprises, by weight, 1-30 parts of aesculus quinquefolius extract, 1-20 parts of sophora flavescens extract, 1-10 parts of cactus extract and 1-30 parts of lithospermum officinale extract; Preferably, the natural ingredient composition comprises, by weight, 5-25 parts of Paridis aestivus extract, 5-18 parts of Sophora flavescens extract, 2-8 parts of Opuntia dioica extract and 5-25 parts of Lithospermum officinale extract.

3. The natural ingredient composition according to claim 1, wherein The natural ingredient composition also includes ophiopogon japonicus extract; Preferably, the mass ratio of the Herba Lysimachiae extract to the Herba Ophiopogonis extract is 1:(0.1-1); Preferably, the mass ratio of the Herba Lysimachiae extract to the Herba Ophiopogonis extract is 1:(0.2-0.5).

4. The natural ingredient composition according to any one of claims 1 to 3, characterized in that The seven-leaf parsley extract, sophora flavescens extract or Xinjiang lithospermum extract is prepared by the following method: seven-leaf parsley, sophora flavescens or Xinjiang lithospermum is crushed and added into polyol, extracted under ultrasound, and the extract is filtered and the clear liquid is collected to obtain the seven-leaf parsley extract, sophora flavescens extract or Xinjiang lithospermum extract.

5. The natural ingredient composition according to claim 4, characterized in that The seven-leaf parsley, sophora flavescens or Xinjiang purple grass are crushed into 50-80 meshes respectively; Preferably, the mass ratio of the Herba Lysimachiae, Sophorae Flavescentis or Lithospermum officinale to the polyol is independently 1:(4-7); Preferably, the polyols each independently include any one of glycerol, 1,2-hexanediol, butanediol or 1,2-pentanediol, or a combination of at least two thereof; Preferably, the polyols are glycerol and 1,2-hexanediol; Preferably, the extraction temperature is independently 40-50°C, and the extraction time is independently 40-80min; Preferably, the frequency of the ultrasound is independently 30-50 KHz; Preferably, the particle sizes of the filter elements used for the filtration are independently 0.15-0.3 μm.

6. The natural ingredient composition according to any one of claims 1 to 5, characterized in that The cactus extract is prepared by the following method: crushing the cactus and mixing it with water, extracting it under heating, filtering the extract and taking the clear liquid as the cactus extract; Preferably, the crushing is to 50-80 mesh; Preferably, the mass ratio of the cactus to water is 1:(5-6); Preferably, the heating is to 90-120°C; Preferably, the extraction time is 1.5-3h; Preferably, the particle size of the filter element used for the filtration is 0.15-0.3 μm.

7. The natural ingredient composition according to any one of claims 3 to 6, characterized in that The Ophiopogon japonicus extract is prepared by the following method: crushing Ophiopogon japonicus and mixing with water, extracting under heating, filtering the extract and taking the clear liquid as the Ophiopogon japonicus extract; Preferably, the crushing is to 50-80 mesh; Preferably, the mass ratio of the ophiopogon japonicus to water is 1:(5-6); Preferably, the heating is to 90-120°C; Preferably, the extraction time is 1.5-3h; Preferably, the particle size of the filter element used for the filtration is 0.15-0.3 μm.

8. A method for preparing a natural ingredient composition having anti-allergic and anti-redness effects as claimed in any one of claims 1 to 7, characterized in that: The preparation method comprises: mixing a seven-leaf parsley extract, a sophora flavescens extract, a cactus extract and a Xinjiang lithospermum officinale extract, and fusing them by high-pressure micro-jetting to obtain the natural ingredient composition.

9. The preparation method according to claim 8, characterized in that: The mixing also includes adding ophiopogon japonicus extract; Preferably, the process conditions of the high-pressure microfluidization are: temperature of 30-45° C. and pressure of 18000-25000 PSI.

10. A cosmetic, characterized in that: The raw material composition of the cosmetics comprises the natural ingredient composition with anti-allergic and anti-redness effects as described in any one of claims 1 to 7; Preferably, the cosmetic is a lotion, a serum or a facial mask; Preferably, the natural ingredient composition with anti-allergic and anti-redness effects is added in an amount of 0.1-20%, preferably 1-10% in cosmetics; Preferably, the raw material composition of the cosmetic further includes additives, and the additives include any one or a combination of at least two of solvents, emulsifiers, moisturizers, preservatives or thickeners.

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