A 377 whitening antioxidant composition and its use in cosmetics

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to a 377 whitening and antioxidant composition and application thereof in cosmetics. In the composition, 377, 577, arbutin, nicotinamide and other components have a whitening effect, and vitamin E, coenzyme Q10 and other components have an antioxidant effect; the lactic acid bacteria fermentation product and the extract of morel have both whitening and antioxidant effects, and the antioxidant effect thereof can be multiplied after being used in combination. Other auxiliary materials such as glycerol and butanediol are used for moisturizing, hydrolyzed collagen is used for repairing, and ethylhexylglycerin and phenoxyethanol are used as preservatives, so as to ensure the stability of the product. These components jointly act to inhibit the formation of melanin in multiple paths, and have good whitening and antioxidant effects.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and specifically relates to a 377 whitening and antioxidant composition and its application in cosmetics. BACKGROUND

[0002] With the increasing attention to skin health, whitening and antioxidant have become a popular research direction in the field of cosmetics. Whitening and antioxidant of the skin not only can improve skin color, but also can delay skin aging, reduce wrinkle and spot formation. Phenylethyl resorcinol (377 for short) as a highly effective whitening ingredient has been widely used in cosmetics. 377 can inhibit the activity of tyrosinase to reduce the generation of melanin, thereby achieving the effect of whitening. In addition, 377 also has good antioxidant performance, which can neutralize free radicals and protect the skin from oxidative damage.

[0003] However, a single whitening ingredient often cannot meet the needs of consumers for comprehensive skin care. Therefore, researchers have begun to explore the compounding of 377 with other whitening and antioxidant ingredients to enhance its efficacy. For example, Chinese invention patent CN11823385B discloses a composition with anti-aging and whitening effects, which includes a plurality of natural plant extracts such as verbascoside, isoverbascoside, forsythia ester glycoside, etc. These ingredients have the effects of whitening and anti-radiation.

[0004] In terms of preparation method, the existing whitening and antioxidant composition mostly adopts a simple mixing process, but this method often cannot fully exert the synergistic effect of each ingredient. Therefore, it is of important practical application value to develop a composition capable of effectively compounding 377 with other whitening and antioxidant ingredients and a preparation method thereof. SUMMARY

[0005] The purpose of the present application is to provide a whitening and antioxidant composition which can significantly improve the whitening effect while exerting higher antioxidant effect, effectively resist the damage of free radicals to the skin, delay skin aging, and more persistently and deeply improve the skin state.

[0006] The present application provides a whitening antioxidant composition, comprising the following components: 377 0.5wt%-2wt%, 577 0.1wt%-1wt%, arbutin 0.2wt%-7wt%, nicotinamide 2wt%-10wt%, lactic acid bacteria fermentation product 1wt%-5wt%, morchella extract 0.1wt%-2wt%, vitamin E 0.1wt%-1wt%, coenzyme Q10 0.01wt%-0.1wt%, glycerol 5wt%-15wt%, butanediol 3wt%-8wt%, sodium hyaluronate 0.05wt%-0.3wt%, polyglutamic acid 0.1wt%-1wt%, betaine 0.5wt%-3wt%, hydrolyzed collagen 1wt%-5wt%, squalane 2wt%-8wt%, ethylhexylglycerin 0.2wt%-1wt%, phenoxyethanol 0.3wt%-1wt% and the balance of water.

[0007] Preferably, the composition comprises the following components: 377 1wt%-2wt%, 577 0.5wt%-1wt%, arbutin 3wt%-5wt%, nicotinamide 5wt%-8wt%, lactic acid bacteria fermentation product 2wt%-4wt%, morchella extract 0.5wt%-1.5wt%, vitamin E 0.2wt%-0.5wt%, coenzyme Q10 0.02wt%-0.05wt%, glycerol 8wt%-12wt%, butanediol 5wt%-7wt%, sodium hyaluronate 0.1wt%-0.2wt%, polyglutamic acid 0.3wt%-0.5wt%, betaine 1wt%-2wt%, hydrolyzed collagen 2wt%-4wt%, squalane 4wt%-6wt%, ethylhexylglycerin 0.5wt%-0.8wt%, phenoxyethanol 0.5wt%-0.8wt% and the balance of water.

[0008] Preferably, the arbutin is alpha-arbutin and / or beta-arbutin.

[0009] Preferably, the alpha-arbutin is added in an amount of 0.2wt%-2wt%, and the beta-arbutin is added in an amount of 1wt%-7wt%.

[0010] The present application also provides the use of the composition described in the above technical solution in the preparation of a whitening antioxidant product.

[0011] The present application also provides a whitening antioxidant product, comprising the composition described in the above technical solution.

[0012] The present application also provides a preparation method of the product described in the above technical solution, comprising the following steps:

[0013] S1, add 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 into butanediol, stir until completely dissolved; then add glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen, mix evenly, then add the remaining water, stir evenly, centrifuge to remove bubbles, and obtain a viscous liquid;

[0014] S2, the viscous liquid is injected into a microneedle mold by vacuumizing, and the liquid is uniformly distributed; after vacuumizing, the excess viscous liquid on the surface of the mold is recovered, and a soluble microcrystalline needle tip is obtained;

[0015] S3, 2-10 parts of chitosan and 4-20 parts of hyaluronic acid solution are stirred evenly, and a base solution is obtained after centrifugation to remove bubbles;

[0016] S4, the base solution is added to the soluble microcrystalline needle tip, and the base solution uniformly covers the needle tip;

[0017] S5, dry at a constant temperature of 35-40 DEG C and a humidity of 15-25% for 12-24 hours, demold after drying, and obtain a soluble microcrystalline with whitening and antioxidant functions.

[0018] Preferably, the stirring rate is 500 rpm / min, and the stirring time is 15-40 min.

[0019] Preferably, the step of centrifuging to remove bubbles is: centrifuging at 4000 rpm / min for 10 min at 4 DEG C.

[0020] Preferably, the molecular weight of the chitosan is 240,000 Da, and the molecular weight of the sodium hyaluronate is 100,000 Da.

[0021] The amount of the base solution is not strictly required, and the amount can be used to uniformly cover the needle tip.

[0022] The beneficial effects of the present application are:

[0023] 377, 577 (4-Butylresorcinol, 4-Butylresorcinol, 577 for short), arbutin, nicotinamide and other ingredients have whitening effect, vitamin E, coenzyme Q10 and other ingredients have antioxidant effect; lactic acid bacteria fermentation product and morchella extract have both whitening and antioxidant effect, and the antioxidant effect can be doubled after the two are used together. Other accessories such as glycerol and butanediol are used for moisturizing, hydrolyzed collagen is used for repair, ethylhexylglycerin and phenoxyethanol are used as preservatives to ensure the stability of the product. These ingredients work together to inhibit the formation of melanin in multiple ways, and have good whitening and antioxidant effect.

[0024] The application embeds the whitening antioxidant composition in the soluble microcrystal needle body compounded by hyaluronic acid and chitin by using microcrystal technology, which is beneficial to the composition to break through the barrier effect and reach the dermis layer, improve the transdermal absorption efficiency, lock the skin moisture, and further improve the whitening and antioxidant effect. DETAILED DESCRIPTION

[0025] In order to further illustrate the application, the application of a 377 whitening antioxidant composition and its application in cosmetics are described in detail below in combination with examples, but they cannot be understood as limiting the protection scope of the application.

[0026] Each substance used in the embodiment of the application is an ingredient recorded in INCI, and the record number information of each component is as follows: 377 (Phenylethyl Resorcinol), 577 (4-Butylresorcinol), Arbutin, Niacinamide, Lactobacillus Ferment Lysate, Morchella Extract, Tocopherol, Ubiquinone, Glycerin, Butylene Glycol, Sodium Hyaluronate, Polyglutamic Acid, Betaine, Hydrolyzed Collagen, Squalane, Ethylhexylglycerin, Phenoxyethanol, Chitosan, Hyaluronic Acid, and Aqua.

[0027] Example 1

[0028] Each component is weighed according to the following amount:

[0029] 377 1g, 577 0.5g, Arbutin 3.5g, Niacinamide 6g, Lactobacillus Ferment Lysate 3g, Morchella Extract 1g, Tocopherol 0.5g, Ubiquinone 0.05g, Glycerin 10g, Butylene Glycol 5.5g, Sodium Hyaluronate 0.15g, Polyglutamic Acid 0.5g, Betaine 1.5g, Hydrolyzed Collagen 3g, Squalane 5g, Ethylhexylglycerin 0.6g, Phenoxyethanol 0.6g, and Aqua 57.6g.

[0030] The 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 are added into butanediol, stirred until completely dissolved; then glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen are added, mixed uniformly, then the remaining water is added, stirred at 500 rpm / min for 20 min, centrifuged at 4℃, 4000 rpm / min for 10 min to remove bubbles, to obtain a viscous liquid;

[0031] The viscous liquid is injected into the microneedle mold by vacuum extraction, ensuring uniform distribution of the liquid; after vacuum extraction, the excess viscous liquid on the surface of the mold is recovered, to obtain soluble microcrystalline needle tips;

[0032] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir uniformly, centrifuge at 4℃, 4000 rpm / min for 10 min to remove bubbles, to obtain a base solution;

[0033] The base solution is added to the soluble microcrystalline needle tips, so that the base solution uniformly covers the needle tips;

[0034] Dry at a constant temperature of 35℃~40℃ and a humidity of 15%~25% for 18h, demold after drying, to obtain soluble microcrystals with whitening and antioxidant functions.

[0035] Example 2

[0036] Each component is weighed according to the following amount:

[0037] 377 1.5g, 577 0.75g, arbutin 4g, nicotinamide 6.5g, lactic acid bacteria fermentation product 3g, morchella extract 1g, vitamin E 0.35g, coenzyme Q10 0.03g, glycerol 10g, butanediol 6g, sodium hyaluronate 0.15g, polyglutamic acid 0.4g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.65g, phenoxyethanol 0.65g, water 55.52g.

[0038] The 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 are added into butanediol, stirred until completely dissolved; then glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen are added, mixed uniformly, then the remaining water is added, stirred at 500 rpm / min for 20 min, centrifuged at 4℃, 4000 rpm / min for 10 min to remove bubbles, to obtain a viscous liquid;

[0039] The viscous liquid is injected into the microneedle mold by vacuum extraction, ensuring uniform distribution of the liquid; after vacuum extraction, the excess viscous liquid on the surface of the mold is recovered, to obtain soluble microcrystalline needle tips;

[0040] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, remove bubbles at 4℃, 4000 rpm / min centrifugation for 10 min, to get the base solution;

[0041] Add the base solution to the soluble microcrystalline needle tip, so that the base solution uniformly covers the needle tip;

[0042] Dry at constant temperature 35℃~40℃, humidity 15%~25% for 18h, demold after drying, to get soluble microcrystalline with whitening and antioxidant function.

[0043] Example 3

[0044] According to the following usage, respectively take each component:

[0045] 377 1g, 577 0.5g, arbutin 3g, nicotinamide 5g, lactic acid bacteria fermentation product 2g, morchella extract 0.5g, vitamin E 0.2g, coenzyme Q10 0.02g, glycerol 8g, butanediol 5g, sodium hyaluronate 0.1g, polyglutamic acid 0.3g, betaine 1g, hydrolyzed collagen 2g, squalane 4g, ethylhexylglycerin 0.5g, phenoxyethanol 0.5g, water 66.38g.

[0046] Add 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 to butanediol, stir until completely dissolved; then add glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen, mix evenly, then add the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4℃, 4000 rpm / min for 10 min to remove bubbles, to get viscous liquid;

[0047] Inject the viscous liquid into the microneedle mold by vacuum extraction, ensure uniform distribution of the liquid; after vacuum extraction, recover the excess viscous liquid on the surface of the mold, to get soluble microcrystalline needle tip;

[0048] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, remove bubbles at 4℃, 4000 rpm / min centrifugation for 10 min, to get the base solution;

[0049] Add the base solution to the soluble microcrystalline needle tip, so that the base solution uniformly covers the needle tip;

[0050] Dry at constant temperature 35℃~40℃, humidity 15%~25% for 18h, demold after drying, to get soluble microcrystalline with whitening and antioxidant function.

[0051] Example 4

[0052] According to the following usage, respectively take each component:

[0053] 377 2g, 577 1g, arbutin 5g, nicotinamide 8g, lactic acid bacteria fermentation product 4g, morchella extract 1.5g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerol 12g, butanediol 7g, sodium hyaluronate 0.2g, polyglutamic acid 0.5g, betaine 2g, hydrolyzed collagen 4g, squalane 6g, ethylhexylglycerin 0.8g, phenoxyethanol 0.8g, and water 44.65g.

[0054] Add 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E, and coenzyme Q10 to butanediol, and stir until completely dissolved; then add glycerol, sodium hyaluronate, polyglutamic acid, betaine, and hydrolyzed collagen, mix well, and then add the remaining water, stir at 500rpm / min for 20min, centrifuge at 4℃, 4000rpm / min for 10min to remove bubbles, and obtain a viscous liquid;

[0055] Inject the viscous liquid into a microneedle mold by vacuum extraction, and ensure that the liquid is evenly distributed; after vacuum extraction, recover the excess viscous liquid on the surface of the mold, and obtain soluble microcrystalline needle tips;

[0056] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir well, centrifuge at 4℃, 4000rpm / min for 10min to remove bubbles, and obtain a base solution;

[0057] Add the base solution to the soluble microcrystalline needle tips, so that the base solution uniformly covers the needle tips;

[0058] Dry at a constant temperature of 35℃~40℃ and a humidity of 15%~25% for 18h, demold after drying, and obtain soluble microcrystals with whitening and antioxidant functions.

[0059] Example 5

[0060] According to the following usage, respectively weigh each component:

[0061] 377 1.2g, 577 0.6g, arbutin 3.5g, nicotinamide 6g, lactic acid bacteria fermentation product 2.5g, morchella extract 0.75g, vitamin E 0.3g, coenzyme Q10 0.03g, glycerol 9g, butanediol 5.5g, sodium hyaluronate 0.12g, polyglutamic acid 0.35g, betaine 1.25g, hydrolyzed collagen 2.5g, squalane 4.5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, and water 60.7g.

[0062] The 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 are added into butanediol, stirred until completely dissolved; then glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen are added, mixed uniformly, then the remaining water is added, stirred at 500 rpm / min for 20 min, centrifuged at 4℃, 4000 rpm / min for 10 min to remove bubbles, to obtain a viscous liquid;

[0063] The viscous liquid is injected into the microneedle mold by vacuum extraction, ensuring uniform distribution of the liquid; after vacuum extraction, the excess viscous liquid on the surface of the mold is recovered, to obtain soluble microcrystalline needle tips;

[0064] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir uniformly, centrifuge at 4℃, 4000 rpm / min for 10 min to remove bubbles, to obtain a base solution;

[0065] The base solution is added to the soluble microcrystalline needle tips, so that the base solution uniformly covers the needle tips;

[0066] Dry at a constant temperature of 35℃~40℃ and a humidity of 15%~25% for 18h, demold after drying, to obtain soluble microcrystals with whitening and antioxidant functions.

[0067] Example 6

[0068] Each component is weighed according to the following amount:

[0069] 377 1.8g, 577 0.85g, arbutin 4.5g, nicotinamide 7g, lactic acid bacteria fermentation product 3.5g, morchella extract 1.25g, vitamin E 0.4g, coenzyme Q10 0.04g, glycerol 11g, butanediol 6.5g, sodium hyaluronate 0.17g, polyglutamic acid 0.45g, betaine 1.75g, hydrolyzed collagen 3.5g, squalane 5.5g, ethylhexylglycerin 0.7g, phenoxyethanol 0.7g, water 50.39g.

[0070] The 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 are added into butanediol, stirred until completely dissolved; then glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen are added, mixed uniformly, then the remaining water is added, stirred at 500 rpm / min for 20 min, centrifuged at 4℃, 4000 rpm / min for 10 min to remove bubbles, to obtain a viscous liquid;

[0071] The viscous liquid is injected into the microneedle mold by vacuum extraction, ensuring uniform distribution of the liquid; after vacuum extraction, the excess viscous liquid on the surface of the mold is recovered, to obtain soluble microcrystalline needle tips;

[0072] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, 4℃, 4000 rpm / min centrifugal 10 min to remove bubbles, get base solution;

[0073] The base solution is added to the soluble microcrystalline needle tip, so that the base solution uniformly covers the needle tip;

[0074] Dry at constant temperature 35℃~40℃, humidity 15%~25% for 18h, after drying, demoulding, get soluble microcrystalline with whitening and antioxidant function.

[0075] Example 7

[0076] According to the following dosage, respectively take each component:

[0077] 377 2g, 577 1g, arbutin 6.5g, nicotinamide 8g, lactic acid bacteria fermentation product 4g, morchella extract 1.5g, vitamin E 0.9g, coenzyme Q10 0.09g, glycerol 12g, butanediol 7g, sodium hyaluronate 0.25g, polyglutamic acid 0.9g, betaine 2.5g, hydrolyzed collagen 4g, squalane 7g, ethylhexyl glycerin 1g, phenoxyethanol 0.8g, water 40.56g.

[0078] Add 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 to butanediol, stir until completely dissolved; then add glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen, mix evenly, then add the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4℃, 4000 rpm / min for 10 min to remove bubbles, get viscous liquid;

[0079] The viscous liquid is injected into the microneedle mold by vacuum pumping, ensuring uniform distribution of the liquid; after vacuum pumping, the excess viscous liquid on the surface of the mold is recovered, and the soluble microcrystalline needle tip is obtained;

[0080] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, 4℃, 4000 rpm / min centrifugal 10 min to remove bubbles, get base solution;

[0081] The base solution is added to the soluble microcrystalline needle tip, so that the base solution uniformly covers the needle tip;

[0082] Dry at constant temperature 35℃~40℃, humidity 15%~25% for 18h, after drying, demoulding, get soluble microcrystalline with whitening and antioxidant function.

[0083] Example 8

[0084] According to the following dosage, respectively take each component:

[0085] 377 1.5g, 577 0.7g, arbutin 5g, nicotinamide 7g, lactic acid bacteria fermentation product 3.5g, morchella extract 1.2g, vitamin E 0.7g, coenzyme Q10 0.07g, glycerol 11g, butanediol 6g, sodium hyaluronate 0.2g, polyglutamic acid 0.7g, betaine 2g, hydrolyzed collagen 3.5g, squalane 6g, ethylhexylglycerin 0.8g, phenoxyethanol 0.7g, water 49.43g.

[0086] Add 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 to butanediol, stir until completely dissolved; then add glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen, mix well, then add the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4℃, 4000 rpm / min for 10 min to remove bubbles, to obtain a viscous liquid;

[0087] The viscous liquid is injected into the microneedle mold by vacuum extraction, ensuring uniform distribution of the liquid; after vacuum extraction, the excess viscous liquid on the surface of the mold is recovered to obtain soluble microcrystalline needle tips;

[0088] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir well, centrifuge at 4℃, 4000 rpm / min for 10 min to remove bubbles, to obtain a base solution;

[0089] Add the base solution to the soluble microcrystalline needle tips so that the base solution uniformly covers the needle tips;

[0090] Dry at a constant temperature of 35℃~40℃ and a humidity of 15%~25% for 18h, then demold to obtain soluble microcrystals with whitening and antioxidant functions.

[0091] Comparative Example 1

[0092] Each component is weighed according to the following amount:

[0093] 377 1g, 577 0.5g, arbutin 3.5g, nicotinamide 6g, lactic acid bacteria fermentation product 4g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerol 10g, butanediol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0094] The 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 are added into butanediol, stirred until completely dissolved; then glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen are added, mixed uniformly, then the remaining water is added, stirred at 500 rpm / min for 20 min, centrifuged at 4℃, 4000 rpm / min for 10 min to remove bubbles, and a viscous liquid is obtained;

[0095] The viscous liquid is injected into the microneedle mold by vacuum extraction, ensuring uniform distribution of the liquid; after vacuum extraction, the excess viscous liquid on the surface of the mold is recovered, and a soluble microcrystalline needle tip is obtained;

[0096] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir uniformly, centrifuge at 4℃, 4000 rpm / min for 10 min to remove bubbles, and a base solution is obtained;

[0097] The base solution is added to the soluble microcrystalline needle tip, so that the base solution uniformly covers the needle tip;

[0098] Dry at a constant temperature of 35℃~40℃ and a humidity of 15%~25% for 18h, demold after drying, and obtain a soluble microcrystalline with whitening and antioxidant functions.

[0099] Comparative Example 2

[0100] Each component is weighed according to the following amount:

[0101] 377 1g, 577 0.5g, arbutin 3.5g, nicotinamide 6g, morchella extract 4g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerol 10g, butanediol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0102] The 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 are added into butanediol, stirred until completely dissolved; then glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen are added, mixed uniformly, then the remaining water is added, stirred at 500 rpm / min for 20 min, centrifuged at 4℃, 4000 rpm / min for 10 min to remove bubbles, and a viscous liquid is obtained;

[0103] The viscous liquid is injected into the microneedle mold by vacuum extraction, ensuring uniform distribution of the liquid; after vacuum extraction, the excess viscous liquid on the surface of the mold is recovered, and a soluble microcrystalline needle tip is obtained;

[0104] Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir uniformly, centrifuge at 4℃, 4000rpm / min for 10min to remove bubbles, and obtain the base solution;

[0105] Add the base solution to the soluble microcrystalline needle tip, so that the base solution uniformly covers the needle tip;

[0106] Dry at a constant temperature of 35℃~40℃ and a humidity of 15%~25% for 18h, demold after drying, and obtain the soluble microcrystalline with whitening and antioxidant functions.

[0107] Comparative Example 3

[0108] Each component is weighed according to the following amount:

[0109] 377 1g, 577 0.5g, arbutin 3.5g, nicotinamide 6g, lactic acid bacteria fermentation product 3g, morchella extract 1g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerol 10g, butanediol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0110] Add 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 to butanediol, stir until completely dissolved; then add glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen, mix uniformly, and then add the remaining water, stir uniformly, and obtain the mixed solution.

[0111] Comparative Example 4

[0112] Each component is weighed according to the following amount:

[0113] 377 1g, 577 0.5g, arbutin 3.5g, nicotinamide 6g, lactic acid bacteria fermentation product 3g, morchella extract 1g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerol 10g, butanediol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0114] Add 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E and coenzyme Q10 to butanediol, stir until completely dissolved; then add glycerol, sodium hyaluronate, polyglutamic acid, betaine, hydrolyzed collagen, mix uniformly, and then add the remaining water, stir uniformly, and obtain the mixed solution.

[0115] The mixture was heated to 80℃, and kept for 2h to make all ingredients fully dissolved, then squalane was added, the temperature of the system was reduced to room temperature after stirring evenly, then ethylhexylglycerin and phenoxyethanol were added, and stirred evenly.

[0116] After the system was cooled to room temperature, the pH value was adjusted to 5.5-6.5, and the homogenizer was used at 5000 rpm for 10 min to obtain the whitening antioxidant essence.

[0117] Test Example 1

[0118] The products prepared in Examples 1-8 and Comparative Examples 1-4 were subjected to safety tests, and the specific method was as follows:

[0119] The patch tester with the test substance was attached to the flexor side of the forearm of the subject with a non-irritating adhesive tape, and the skin was pressed evenly with the palm for 24h. Observation was made immediately after application, and repeated observation and scoring were made at 24h, 48h and 72h, and the symptoms such as erythema, edema and their degrees were recorded.

[0120] The scoring criteria for skin irritation test were as follows:

[0121] Erythema and eschar formation:

[0122] No erythema: 0 points

[0123] Mild erythema (barely visible): 1 point

[0124] Obvious erythema: 2 points

[0125] Moderate-severe erythema: 3 points

[0126] Severe erythema (purple red) to mild eschar formation: 4 points

[0127] Edema formation:

[0128] No edema: 0 points

[0129] Mild edema (barely visible): 1 point

[0130] Mild edema (skin elevation profile clear): 2 points

[0131] Moderate edema (skin elevation about 1mm): 3 points

[0132] Severe edema (skin elevation more than 1mm, range expansion): 4 points

[0133] The maximum score for skin irritation reaction score was 8 points. According to the average of the highest score at 24h, 48h and 72h observation time points, the skin irritation intensity was determined:

[0134] Score average 6.0-8.0: strong irritation

[0135] Integral mean value of 2.0<6.0: moderate irritation

[0136] Integral mean value of 0.5<2.0: mild irritation

[0137] Integral mean value of 0<0.5: no irritation

[0138] The results show that the skin reactions of the subjects are all no irritation, indicating that the products involved in the present application are all mild and safe.

[0139] Test Example 2

[0140] The products prepared in Examples 1 to 8 and Comparative Examples 1 to 4 were used as samples, and sample solutions of different concentrations were prepared. In a 96-well plate, 100 μL of the sample solution was added to each well. 100 μL of tyrosinase solution (0.1 U / mL) was added to each well. 100 μL of substrate solution (2 mM L-tyrosine or L-dopa) was added to each well. Incubation was performed at 37°C for 30 min. The absorbance value was measured at a wavelength of 475 nm, and the tyrosinase inhibition rate was calculated according to the following formula;

[0141] Inhibition rate (%) = (1 - sample solution absorbance value / control group absorbance value) x 100%

[0142] Melanoma cells were seeded in a 96-well plate at 5000 cells per well. Different concentrations of the test substance solution were added. Incubation was performed at 37°C, 5% CO2 for 48 hours. Spectrophotometry or image analysis technology was used to determine the intracellular melanin content, and the melanin content reduction rate was calculated according to the following formula, and the results are shown in Table 1.

[0143] Reduction rate (%) = (1 - sample group melanin content / control group melanin content) x 100%

[0144] Table 1 Whitening effect verification test results

[0145]

[0146] As can be seen from Table 1, compared with Comparative Examples 1 to 3, the products of Example 1 and Comparative Example 4 have higher tyrosinase inhibition rate and melanin reduction rate, indicating that the combination of lactic acid bacteria fermentation product and Morchella extract has better whitening effect.

[0147] Test Example 3

[0148] 1. DPPH free radical scavenging experiment

[0149] The products prepared in Examples 1 to 8 and Comparative Examples 1 to 4 were used as samples, and sample solutions of different concentrations were prepared.

[0150] In the 96-well plate, 100 μL of sample solution was added to each well, and then 100 μL of DPPH solution was added to each well.

[0151] After incubation at room temperature for 30 min in the dark, the absorbance value was measured at a wavelength of 517 nm, and the DPPH free radical scavenging rate was calculated according to the following formula; the results are shown in Table 2.

[0152] Scavenging rate (%) = (1 - absorbance value of test sample / absorbance value of control group) x 100%

[0153] 2. Oxygen radical absorbance capacity experiment

[0154] The products prepared in Examples 1 to 8 and Comparative Examples 1 to 4 were used as samples, and sample solutions of different concentrations were prepared.

[0155] In the 96-well plate, 25 μL of sample solution was added to each well, followed by the addition of 150 μL of AAPH solution (153 mM) to each well, and then 25 μL of fluorescein solution (81 nM) was added to each well.

[0156] After incubation at 37°C in the dark, the fluorescence intensity was measured at an excitation wavelength of 485 nm and an emission wavelength of 528 nm every 15 min for a total of 120 min.

[0157] The reduction rate of fluorescence intensity was calculated according to the following formula, and the results are shown in Table 2.

[0158] Reduction rate (%) = (1 - fluorescence intensity of test sample group / fluorescence intensity of control group) x 100%

[0159] Table 2. Results of antioxidant test

[0160]

[0161] As can be seen from Table 2, compared to Comparative Examples 1 to 2, the product of Example 1 has a higher DPPH free radical scavenging rate and a lower fluorescence intensity reduction rate, indicating that the composition and product provided by the present application have better antioxidant effect.

[0162] Test Example 4

[0163] Transdermal absorption test

[0164] Volunteers were recruited: 120 people, female, aged 18-50 years old, healthy, no medication.

[0165] Grouping: the volunteers were randomly divided into 12 groups, 10 people in each group. Test groups 1-12 were given products prepared in Examples 1 to 8 and Comparative Examples 1 to 4, respectively, and a blank control area was set.

[0166] Test site: face.

[0167] Test instrument: skin test instrument, skin moisture loss (TEWL) test probe.

[0168] Test sample and blank control: sample application area and blank control area are randomly distributed on the left and right faces.

[0169] Measurement method: first measure the blank value of each test area, then apply the product to the test area according to the specified usage and dosage.

[0170] Test time: 4 weeks. Before use, 2 weeks, 4 weeks, respectively measure the change of skin moisture loss content of test area and blank control area, by measuring the TEWL value before and after use, calculate the TEWL change rate according to the following formula to evaluate the moisturizing and barrier repair effect of the product, the results are shown in Table 3.

[0171] TEWL change rate (%) = (1-TEWL value after use / TEWL value before use) x 100%

[0172] Table 3 TEWL change rate

[0173]

[0174] From Table 3, compared with Comparative Example 3 and Comparative Example 4, the product prepared by Example 1-Example 8 has a higher TEWL change rate, which shows that the product provided by the present application can effectively inhibit the skin moisture loss, and the present application through the microcrystalline technology not only significantly improves the absorption efficiency of the product, but also endows the product with excellent moisturizing performance, and also performs excellent in repairing skin barrier.

[0175] Although the above examples make a detailed description of the present application, it is only a part of the embodiments of the present application, not all the embodiments, and people can also obtain other embodiments according to the present embodiment without creativity, which all belong to the protection scope of the present application.

Claims

1. A whitening antioxidant composition characterized in that, The composition comprises the following components: 377 0.5wt%-2wt%, 577 0.1wt%-1wt%, arbutin 0.2wt%-7wt%, nicotinamide 2wt%-10wt%, lactic acid bacteria fermentation product 1wt%-5wt%, morchella extract 0.1wt%-2wt%, vitamin E 0.1wt%-1wt%, coenzyme Q10 0.01wt%-0.1wt%, glycerol 5wt%-15wt%, butanediol 3wt%-8wt%, sodium hyaluronate 0.05wt%-0.3wt%, polyglutamic acid 0.1wt%-1wt%, betaine 0.5wt%-3wt%, hydrolyzed collagen 1wt%-5wt%, squalane 2wt%-8wt%, ethylhexylglycerin 0.2wt%-1wt%, phenoxyethanol 0.3wt%-1wt%, and the balance of water.

2. The composition of claim 1, wherein, The composition comprises the following components: 377 1wt%-2wt%, 577 0.5wt%-1wt%, arbutin 3wt%-5wt%, nicotinamide 5wt%-8wt%, lactic acid bacteria fermentation product 2wt%-4wt%, morchella extract 0.5wt%-1.5wt%, vitamin E 0.2wt%-0.5wt%, coenzyme Q10 0.02wt%-0.05wt%, glycerol 8wt%-12wt%, butanediol 5wt%-7wt%, sodium hyaluronate 0.1wt%-0.2wt%, polyglutamic acid 0.3wt%-0.5wt%, betaine 1wt%-2wt%, hydrolyzed collagen 2wt%-4wt%, squalane 4wt%-6wt%, ethylhexylglycerin 0.5wt%-0.8wt%, phenoxyethanol 0.5wt%-0.8wt%, and the balance of water.

3. The composition according to claim 1 or 2, wherein The arbutin is α-arbutin and / or β-arbutin.

4. The composition of claim 3, wherein, The addition amount of the α-arbutin is 0.2wt%-2wt%, and the addition amount of the β-arbutin is 1wt%-7wt%.

5. Use of the composition of any one of claims 1-4 in the preparation of a whitening antioxidant product.

6. A whitening antioxidant product, characterized by, The product comprises the composition of any one of claims 1-4.

7. Process for the preparation of the product according to claim 6, characterized in that, The method comprises the following steps: S1, adding 377, 577, arbutin, nicotinamide, lactic acid bacteria fermentation product, morchella extract, vitamin E, and coenzyme Q10 into butanediol, stirring until completely dissolved; then adding glycerol, sodium hyaluronate, polyglutamic acid, betaine, and hydrolyzed collagen into the remaining water, stirring uniformly, removing bubbles by centrifugation, and obtaining a viscous liquid; S2, injecting the viscous liquid into a microneedle mold through vacuum extraction, ensuring uniform distribution of the liquid; recovering the excess viscous liquid on the surface of the mold after vacuum extraction, and obtaining soluble microcrystalline needle tips; S3, taking 2-10 parts of chitosan and 4-20 parts of hyaluronic acid solution, stirring uniformly, removing bubbles by centrifugation, and obtaining a base solution; S4, adding the base solution to the soluble microcrystalline needle tips, and making the base solution uniformly cover the needle tips; S5, drying under the condition of constant temperature 35-40 DEG C, humidity 15-25%, for 12-24h, demolding after drying, to obtain soluble microcrystalline with whitening and antioxidant function.

8. The preparation method according to claim 7, characterized in that, The stirring rate is 500 rpm / min, and the stirring time is 15-40 min.

9. The preparation method according to claim 7, characterized in that, The step of removing bubbles by centrifugation is centrifugation at 4000 rpm / min for 10 min at 4 DEG C.

10. The preparation method according to claim 7, characterized in that, The molecular weight of the chitosan is 240,000 Da, and the molecular weight of the sodium hyaluronate is 100,000 Da.

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