Traditional Chinese medicine composition for preventing or treating diarrhea as well as preparation method and application thereof

Abstract

The invention discloses a traditional Chinese medicine composition for preventing or treating diarrhea of livestock and poultry. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-20 parts of gallnut, 6-15 parts of scutellaria baicalensis, 6-15 parts of humifuse euphorbia herb, 4-10 parts of sophora flower, 10-15 parts of codonopsis pilosula, 6-15 parts of poria cocos, 5-10 parts of fructus psoraleae and 3-5 parts of liquorice. The traditional Chinese medicine composition provided by the invention has the effects of clearing away heat and toxic materials, eliminating dampness and stopping diarrhea, cooling blood and stopping bleeding, promoting diuresis and tonifying spleen, and tonifying qi and strengthening body resistance; meanwhile, the medicine is safe, free of toxic and side effects and capable of strengthening body resistance and eliminating pathogenic factors. The traditional Chinese medicine composition provided by the invention is remarkable in curative effect, low in cost and convenient to use, the diarrhea condition is remarkably relieved after the traditional Chinese medicine composition is fed for 3-7 days, and the traditional Chinese medicine composition can effectively replace antibiotic drugs.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese veterinary medicine, and specifically relates to a traditional Chinese medicine composition and a preparation method thereof and application thereof in preventing or treating diarrhea. Background Art

[0002] At present, in animal husbandry, animal digestive tract syndrome occurs frequently, especially diarrhea characterized by increased defecation frequency and changes in feces shape, which leads to stunted growth and development, low immunity, increased mixed infection and secondary infection, and even death in severe cases, causing huge economic losses to the breeding industry. Antibiotics have a certain effect on them, but long-term and widespread use has led to serious drug resistance problems, which has brought great difficulties to the prevention and treatment of diseases; and excessive drug residues in animal products have affected the quality and safety of products, threatening the health of consumers. Drug-resistant bacteria and drug residues also pollute the ecological environment, seriously affecting the health of humans and animals. Traditional Chinese medicine is mostly derived from green natural plants. It has many advantages such as safety, no drug residues, and not easy to produce drug resistance. It is a preferred alternative to antibiotics.

[0003] How to develop highly effective, safe, and low-drug residue green and environmentally friendly drugs has become a focal issue that needs to be urgently addressed in the prevention and treatment of animal diarrhea. Summary of the invention

[0004] Purpose of the invention: In view of the deficiencies in the prior art, the present invention provides a Chinese medicine composition for preventing or treating diarrhea, which has the effects of clearing away heat and detoxifying, drying dampness and stopping diarrhea, cooling blood and stopping bleeding, promoting dampness and strengthening the spleen, and invigorating qi and strengthening the body. It is safe, has no toxic side effects, strengthens the body and eliminates evil, and treats both the symptoms and the root causes. It can consolidate the exterior and stop diarrhea, improve digestive tract function, and reduce the drug resistance problem caused by the use of antibiotics.

[0005] In order to solve the above technical problems, the present invention discloses a traditional Chinese medicine composition for preventing or treating diarrhea in livestock and poultry, comprising the following components in parts by weight: 10-20 parts of Galla chinensis, 6-15 parts of Scutellaria baicalensis, 6-15 parts of Euphorbia pulegosae, 4-10 parts of Sophora japonicae, 10-15 parts of Codonopsis pilosula, 6-15 parts of Poria cocos, 5-10 parts of Psoralea corylifolia and 3-5 parts of Licorice.

[0006] Preferably, the traditional Chinese medicine composition comprises the following components in parts by weight: 12-16 parts of Galla chinensis, 6-10 parts of Scutellaria baicalensis, 6-10 parts of Euphorbia pulegosa, 4-8 parts of Sophora japonica, 12-15 parts of Codonopsis pilosula, 10-13 parts of Poria cocos, 5-8 parts of Psoralea corylifolia, and 3-5 parts of Licorice.

[0007] In a preferred embodiment, the Chinese medicine composition comprises the following components in parts by weight: 15 parts of Galla chinensis, 8 parts of Scutellaria baicalensis, 8 parts of Euphorbia pulegosa, 6 parts of Sophora japonica, 12 parts of Codonopsis pilosula, 12 parts of Poria cocos, 6 parts of Boneless Bone Nodule, and 3 parts of Licorice.

[0008] Wherein, the dosage form of the Chinese medicine composition is any one of decoction, powder, granule, powder, pill or capsule.

[0009] The present invention further proposes a method for preparing the above-mentioned Chinese medicine composition, comprising the following steps: weighing each component by weight, soaking in water, then decocting twice, filtering and combining the medicinal liquid.

[0010] The above preparation method may further include a concentration process and / or a drying process according to the requirements of the dosage form, or further add common auxiliary materials to prepare powders, granules, powders, pills or capsules.

[0011] Preferably, the soaking in water is to add 8-10 times the total weight of the drug in water and soak at 4-6°C for 10-12 hours.

[0012] The present invention further proposes the use of the above-mentioned traditional Chinese medicine composition in the preparation of veterinary drugs, feed additives or feed for preventing or treating diarrhea.

[0013] Specifically, when preparing feed for preventing or treating diarrhea, the Chinese medicine composition is prepared into a commonly used dosage form and added into the feed to obtain the medicated feed.

[0014] Preferably, the animal used for the Chinese medicine composition is cattle, sheep or pig.

[0015] Specifically, when preparing a feed additive or feed for preventing or treating diarrhea, the dosage of the Chinese medicine composition is 3-40 g / kg of feed based on complete feed; when preparing a Chinese medicine preparation for preventing or treating diarrhea, the daily direct feeding dose of the Chinese medicine composition is 0.2-0.5 g / kg body weight, which can be taken in divided doses or all at once.

[0016] The functional characteristics of each raw material in the Chinese medicine composition of the present invention are as follows:

[0017] Gallnut: tastes sour, astringent and cold; enters the lung, kidney and large intestine meridians; has the effects of astringing the intestines to stop diarrhea, astringing and stopping bleeding, astringing the lungs to reduce fire, and astringing dampness and healing sores; used for long-term diarrhea, dysentery with blood, long-term cough due to lung deficiency, sores, swelling and infection, and wet and rotten skin.

[0018] Scutellaria baicalensis: bitter and cold. Enters the lung, gallbladder, spleen, large intestine and small intestine meridians; has the functions of clearing away heat and dampness, purging fire and detoxifying, stopping bleeding and calming the fetus; used for damp-heat diarrhea, damp-heat stranguria, lung-heat cough, bloody stool, carbuncle, swelling and ulcer.

[0019] Herba Dijincao: spicy and neutral in taste; enters the liver and large intestine meridians; has the effects of clearing away heat and detoxifying, cooling blood and stopping bleeding; used for diarrhea, bloody stool, hematuria, edema and malignant ulcers.

[0020] Sophora flower: bitter and slightly cold; enters the liver and large intestine meridians; has the effects of cooling blood, stopping bleeding, stopping dysentery, clearing the liver and purging fire; used for bloody stools and red eyes.

[0021] Codonopsis: sweet and neutral in taste; enters the spleen and lung meridians; has the effects of tonifying the middle and replenishing qi, strengthening the spleen and benefiting the lungs; used for diarrhea due to spleen deficiency, cough due to lung deficiency, and collapsing due to qi deficiency.

[0022] Poria: sweet, light, and neutral. It enters the heart, lung, spleen, and kidney meridians. It has the effects of promoting diuresis and eliminating dampness, strengthening the spleen, and calming the mind. It is used for edema, oliguria, phlegm and fluid, palpitations, spleen deficiency, poor appetite, loose stools, restlessness, palpitations, and insomnia.

[0023] Psoralea corylifolia: It tastes pungent, bitter and warm; it enters the kidney and spleen meridians; it has the effects of warming the kidney and strengthening yang, stopping diarrhea and absorbing qi; it is used for spermatorrhea, frequent urination, stopping diarrhea and relieving cough and asthma.

[0024] Licorice: sweet and neutral in taste; enters the heart, lung, spleen, and stomach meridians; has the functions of tonifying the spleen and replenishing Qi, removing phlegm and relieving cough, harmonizing the stomach and relieving urgency, detoxifying, alleviating drug toxicity and potency, and harmonizing various medicines.

[0025] The present invention is formulated according to the principle of syndrome differentiation and treatment in traditional Chinese veterinary medicine. Among them, the main drug is gallnut, which can astringe the intestines and stop diarrhea, astringe and stop bleeding; the ministerial drugs are scutellaria, herba euphorbiae, and flos sophorae, which can clear away heat and detoxify, dry dampness and stop diarrhea, cool blood and stop bleeding, stop diarrhea and stop dysentery; the auxiliary drugs are codonopsis, tuckahoe and psoralea corylifolia, which can invigorate qi and strengthen the spleen, promote dampness and stop diarrhea; and the guiding drug is liquorice, which can harmonize all the drugs.

[0026] Beneficial effects: The Chinese medicine composition provided by the present invention has the effects of clearing away heat and detoxifying, drying dampness and stopping diarrhea, cooling blood and stopping bleeding, promoting dampness and strengthening the spleen, and invigorating qi and strengthening the body; at the same time, it is safe, has no toxic side effects, strengthens the body and eliminates evil, and treats both the symptoms and the root causes. The Chinese medicine composition provided by the present invention has significant efficacy, low cost, and is easy to use. After taking the medicine, the diarrhea situation is significantly alleviated, and it can effectively replace antibiotics. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The single drug sensitivity test results in Example 1;

[0028] Figure 2 The results of the drug sensitivity test of the Chinese medicine composition in Example 2;

[0029] Figure 3 The clinical characteristics of mice in Example 3;

[0030] Figure 4 This is a picture of the feces of mice in Example 4;

[0031] Figure 5 The percentage reduction of loose stool rate of mice in Example 4. Note: One-way ANOVA was used for significant difference test. Different letters indicate significant difference between groups at the same time point.

[0032] Figure 6 These are the pathological changes in the intestinal tract of mice in Example 4. DETAILED DESCRIPTION

[0033] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more clear.

[0034] Example 1 Screening of in vitro antibacterial Chinese medicine.

[0035] 1.1 Test strains and culture medium

[0036] Pathogenic Escherichia coli was isolated, cultured, identified and selected from clinical calf diarrhea cases. The culture medium was common broth medium, nutrient agar medium, MacConkey medium, and eosin-methylene blue agar medium.

[0037] 1.2 Preparation of experimental medicinal materials and drug sensitivity papers

[0038] Weigh 20 g each of rhubarb, ebony, gallnut, dandelion, scutellaria, sophora japonica, coptis chinensis, herba euphorbiae, and honeysuckle, add 8 times the total mass of water, soak at 4°C for 10 h, decoct twice, filter the liquid, combine, concentrate to 1 g / mL, sterilize under high pressure, and store in a refrigerator at 4°C for later use.

[0039] Place 50 sterile blank drug-susceptibility papers in a flat dish under sterile conditions, use a pipette to drop 1 mL of the above-mentioned Chinese medicine solution onto the drug-susceptibility papers, then place them in a 37°C oven to dry for 48 hours, take them out and put them into a sterile brown small glass bottle, cover the bottle cap tightly, and store them at 4°C for later use.

[0040] 1.3 Drug sensitivity test

[0041] In the clean bench, prepare the calf diarrhea Escherichia coli isolate into a 0.5 McFarland turbidity bacterial suspension, take 200 μL and use a sterile cotton test to evenly spread the bacterial solution on the nutrient agar medium using a four-step method of horizontal, vertical, oblique and two peripheral circles, and let it stand for 3-5 minutes. Use sterile tweezers to stick the drug-sensitive paper on the surface of the culture medium, press the paper lightly, let it stand for 15 minutes, then invert the plate and place it in a 37°C constant temperature culture. After 18-24 hours of culture, observe whether there is an antibacterial circle around the drug-sensitive paper, measure the diameter of the antibacterial circle, make a record, and repeat three times.

[0042] 1.4 Criteria for determining the antibacterial effect of a single Chinese herbal medicine

[0043] At present, there is still no unified judgment standard for Chinese medicine sensitivity test at home and abroad. Therefore, the antibacterial effect is judged based on the judgment standards reported in the literature of similar tests. The diameter of the inhibition zone ≥20mm is extremely sensitive, 15-20mm is highly sensitive, 10-15mm is moderately sensitive, and less than 10mm is low sensitive.

[0044] 1.5 Results of antibacterial effects of single drugs

[0045] Black plum, dandelion and honeysuckle did not form an antibacterial zone; while rhubarb, gallnut, scutellaria, sophora japonica, coptis chinensis and ground ivy all formed an antibacterial zone.

[0046] Depend on Figure 1 It can be seen that rhubarb, gallnut, scutellaria, sophora japonica, coptis chinensis and herbaceous wormwood all have antibacterial effects in vitro, and the average diameters of the inhibition zones are 8.00mm, 23.33mm, 16.67mm, 11.00mm, 12.67mm and 15.33mm, respectively. Among them, gallnut has the strongest antibacterial effect and is extremely sensitive; scutellaria and herbaceous wormwood have relatively strong antibacterial effects and are highly sensitive; coptis chinensis and sophora japonica have relatively weak antibacterial effects and are moderately sensitive; and rhubarb has the weakest antibacterial effect and is hyposensitive.

[0047] Example 2 Screening of antibacterial effects of Chinese medicine compositions

[0048] On the basis of the above-mentioned Chinese medicine components with good antibacterial effects, in order to further explore the in vitro antibacterial effects of the above-mentioned Chinese medicine components under the synergistic effect, Chinese medicine components with strong antibacterial effects such as Galla chinensis, Scutellaria baicalensis, Sophora japonica, Coptis chinensis and Herba Euphorbiae were selected according to the antibacterial effects of the above-mentioned single drugs for compatibility and combination research. The weight proportions of the Chinese medicine composition are as follows:

[0049] Chinese medicine composition 1: 15 parts of Galla chinensis, 8 parts of Scutellaria baicalensis, and 8 parts of Coptis chinensis.

[0050] Weigh each component according to the weight ratio, add water 8 times the total weight of the drug, soak at 4°C for 10 h, decoct twice, filter and combine the drug solution, concentrate to a concentration of 1 g / mL, and sterilize by high pressure.

[0051] Chinese medicine composition 2: 15 parts of Galla chinensis, 8 parts of Scutellaria baicalensis, and 8 parts of Herba Euphorbiae.

[0052] Weigh each component according to the weight ratio, add water 8 times the total weight of the drug, soak at 4°C for 10 h, decoct twice, filter and combine the drug solution, concentrate to a concentration of 1 g / mL, and sterilize by high pressure.

[0053] Chinese medicine composition 3: 15 parts of Galla chinensis, 8 parts of Scutellaria baicalensis, 8 parts of Coptis chinensis, and 6 parts of Sophora japonica.

[0054] Weigh each component according to the weight ratio, add water 8 times the total weight of the drug, soak at 4°C for 10 h, decoct twice, filter and combine the drug solution, concentrate to a concentration of 1 g / mL, and sterilize by high pressure.

[0055] Chinese medicine composition 4: 15 parts of Galla chinensis, 8 parts of Scutellaria baicalensis, 8 parts of Euphorbia pulcherrima, and 6 parts of Sophora japonica.

[0056] Weigh each component according to the weight ratio, add water 8 times the total weight of the drug, soak at 4°C for 10 h, decoct twice, filter and combine the drug solution, concentrate to a concentration of 1 g / mL, and sterilize by high pressure.

[0057] Investigation on the in vitro antibacterial effect of each group of Chinese medicine compositions:

[0058] The in vitro antibacterial effect was determined by the Oxford cup method. The steps of the Oxford cup method are as follows: the isolated Escherichia coli from calf diarrhea was prepared into a 0.5 McFarland turbidity bacterial suspension, 200 μL was taken and the bacterial suspension was evenly spread on the nutrient agar medium using a sterile cotton test piece using a four-step method of horizontal, vertical, oblique and two peripheral circles, and the clean bench was left to stand for 3-5 minutes. Use sterile tweezers to clamp the sterile Oxford cup vertically on the nutrient agar medium coated with the bacterial solution, and press the cup hard to make it completely close to the bottom of the plate. Use a pipette to add 20 μL of the drug solution to the Oxford cup, place it in a 37°C constant temperature incubator for constant temperature culture for 18-24 hours, and observe and measure the diameter of the antibacterial circle around the Oxford cup. Repeat 3 times.

[0059] Depend on Figure 2 It can be seen that Chinese medicine compositions 1-4 have different in vitro antibacterial effects. The average diameters of the antibacterial zones are 14.67 mm, 18.00 mm, 12.33 mm, and 20.33 mm, respectively, among which Chinese medicine composition 4 has the strongest antibacterial effect.

[0060] Example 3 Prescription and preparation of a Chinese medicine composition for preventing and treating diarrhea

[0061] Based on the screening results of the antibacterial effect of the Chinese medicine composition in Example 2, the Chinese medicine composition 4 with the best antibacterial efficacy was selected. At the same time, following the treatment principle of treating both the symptoms and the root causes, and giving equal importance to strengthening the body and eliminating evil in the theory of traditional Chinese veterinary medicine, the Chinese medicine composition was scientifically formulated with Codonopsis pilosula, Poria cocos, Psoralea corylifolia and Licorice, which have the effects of invigorating qi, removing dampness and strengthening the spleen, according to the principle of monarch, minister, assistant and messenger prescription, to achieve mutual synergy between the components. At the same time, by adjusting the dosage ratio of each component, the effects of different monarch, minister, assistant and messenger compatibility schemes on the diarrhea model were further explored. The selected components of the Chinese medicine composition and their weight proportions are as follows:

[0062] Chinese medicine composition 5: 10 parts of Galla chinensis, 12 parts of Scutellaria baicalensis, 12 parts of Euphorbia pulegosae, 8 parts of Sophora japonica, 10 parts of Codonopsis pilosula, 10 parts of Poria cocos, 6 parts of Psoralea corylifolia, and 3 parts of Licorice.

[0063] This prescription, based on Chinese medicine composition 4, adds Codonopsis pilosula, Poria cocos, Psoralea corylifolia and Licorice, while increasing the dosage of Scutellaria baicalensis, Herba Euphorbiae Herba and Flos Sophorae, and reducing the dosage of Galla chinensis.

[0064] Weigh each component in proportion to its weight, add water 8 times the total weight of the drug, soak at 4°C for 10 hours, decoct twice, filter and combine the drug solution, concentrate to a concentration of 1 g / mL, and sterilize under high pressure. The formula is easy to prepare and use.

[0065] Chinese medicine composition 6: 15 parts of Galla chinensis, 8 parts of Scutellaria baicalensis, 8 parts of Herba Euphorbiae Ichthyophthirius, 6 parts of Sophora japonicae, 12 parts of Codonopsis pilosula, 12 parts of Poria cocos, 6 parts of Psoralea corylifolia, and 3 parts of Licorice.

[0066] This prescription, on the basis of Chinese medicine composition 4, adds Codonopsis pilosula, Poria cocos, Psoralea corylifolia and Licorice, and at the same time increases the dosage of Codonopsis pilosula and Poria cocos compared to Chinese medicine composition 5.

[0067] Weigh each component in proportion to its weight, add water 8 times the total weight of the drug, soak at 4°C for 10 hours, decoct twice, filter and combine the drug solution, concentrate to a concentration of 1 g / mL, and sterilize under high pressure. The formula is easy to prepare and use.

[0068] Chinese medicine composition 7: 20 parts of Galla chinensis, 6 parts of Scutellaria baicalensis, 6 parts of Euphorbia pulegosae, 4 parts of Sophora japonica, 15 parts of Codonopsis pilosula, 15 parts of Poria cocos, 10 parts of Psoralea corylifolia, and 3 parts of Licorice.

[0069] This prescription, based on Chinese medicine composition 4, adds Codonopsis pilosula, Poria cocos, Psoralea corylifolia and Licorice, and at the same time increases the dosage of Galla chinensis, reduces the dosage of Scutellaria baicalensis, Herba Euphorbiae Iris and Sophora japonicae, and increases the dosage of Codonopsis pilosula, Poria cocos and Psoralea corylifolia compared to Chinese medicine composition 6.

[0070] Weigh each component according to the weight ratio, add water 8 times the total weight of the drug, soak at 4°C for 10 h, decoct twice, filter and combine the drug solution, concentrate to a concentration of 1 g / mL, sterilize under high pressure, and store for later use.

[0071] Example 4 Effects of each Chinese medicine composition in preventing and treating diarrhea

[0072] 4.1 Animal experiments

[0073] Six-week-old healthy mice (half male and half female) were selected and randomly divided into 5 groups, namely control group, model group, Chinese medicine group 1, Chinese medicine group 2 and Chinese medicine group 3. There were 10 mice in each group (half male and half female), which were numbered, weighed, fed and drank freely, and managed according to routine procedures. Mice in the model group and Chinese medicine group were intraperitoneally injected with 5×10 8 CFU / mL calf diarrhea Escherichia coli isolate suspension 0.2mL, the control group was injected with double distilled water 0.2mL. 1h after infection, Chinese medicine group 1, Chinese medicine group 2 and Chinese medicine group 3 were respectively gavaged with Chinese medicine composition 5, Chinese medicine composition 6 and Chinese medicine composition 7 in Example 3, 0.2mL / mouse / day, once a day, for 3 consecutive days. The clinical symptoms, fecal characteristics, number of defecation and mortality of mice were observed every day, and the percentage reduction of loose stool rate in each group of mice after medication was calculated. After the experiment, the pathological changes in the intestinal tract of mice were observed by dissection.

[0074] According to the state of mouse feces, they are divided into: dry feces, normal-looking but watery feces, abnormal-looking soft feces, mucous feces and watery feces. Dry feces and normal-looking but watery feces are normal feces, while abnormal-looking soft feces, mucous feces and watery feces are loose feces.

[0075]

[0076] 4.2 Changes in clinical characteristics of mice

[0077] The mice in the control group maintained a good mental state, with shiny fur and quick reactions. Six hours after infection, the mice in the model group showed huddling, messy fur, dullness, squinting, and mental fatigue. After 36 hours, the symptoms worsened, and eye discharge, anal congestion, and slow reaction appeared. Three mice died, with a mortality rate of 30%. Six hours after infection, the mice in the Chinese medicine group 1 showed messy fur, mental fatigue, and huddling. After 24 hours, some mice trembled and squinted. Two mice died, with a mortality rate of 20%. Six hours after infection, the mice in the Chinese medicine group 2 showed ruffled fur, curled up, and some squinted. After 36 hours, one mouse died (mortality rate of 10%). After 54 hours, the huddling situation was alleviated, and the mental state and reaction were basically normal. Six hours after infection, the mice in the Chinese medicine group 3 showed mental fatigue, messy fur, and huddling. After 30 hours, some mice were dull and squinted. One mouse died, with a mortality rate of 10% ( Figure 3 ).

[0078] 4.3 Diarrhea in mice

[0079] like Figure 4 As shown in the figure, the feces of mice in the control group were normal in appearance, granular, and without loose stools. The number of defecations of mice in the model group gradually increased, and the feces gradually showed abnormal loose and soft stools, or loose and unformed feces. The number of defecations of mice in the TCM group increased on the first day, and then gradually slowed down. The feces of mice in TCM group 1 were loose, soft and deformable, and some feces were unformed; the feces of mice in TCM group 2 gradually hardened, and the appearance was basically normal, granular, and some were moist and easy to stick; some feces of mice in TCM group 3 gradually became granular in appearance, and some became loose and soft and deformed.

[0080] Depend on Figure 5 It can be seen that on the 1st, 2nd and 3rd days, compared with the model group, the average loose stool rate of mice in the Chinese medicine group 1 decreased by 10.9%, 25.0% and 31.6%, respectively, the average loose stool rate of mice in the Chinese medicine group 2 decreased by 25.8%, 56.4% and 72.6%, respectively, and the average loose stool rate of mice in the Chinese medicine group 3 decreased by 15.5%, 29.9% and 39.4%, respectively. The results showed that the loose stool rate of mice in the Chinese medicine group 2 decreased significantly, which was significantly higher than that of the Chinese medicine groups 1 and 3 (P<0.05), indicating that the Chinese medicine composition 6 has a significant improvement effect on diarrhea in mice and has the best efficacy.

[0081] 4.4 Intestinal pathological changes in mice

[0082] Depend on Figure 6 It can be seen that the intestinal contents of the mice in the control group were normal and granular, and there was no bleeding or congestion in the intestinal mucosa. The intestinal mucosa of the mice in the model group was congested and bleeding, and the intestinal contents were light yellow and thin and watery. The intestinal contents of the mice in the Chinese medicine group 1 were yellow and thin, and some were thin and soft. There were no obvious bleeding lesions in the intestines of the mice in the Chinese medicine group 2, and the intestinal contents were normal and granular. The intestinal bleeding of the mice in the Chinese medicine group 3 was accompanied by bubbles, and part of the contents were yellow and thin. The results show that the Chinese medicine composition 6 can effectively repair the intestinal lesions of the diseased mice, improve the digestive function, and has the best therapeutic effect on diarrhea.

[0083] In summary, the Chinese medicine composition of the present application has a good therapeutic effect for diarrhea, especially the Chinese medicine composition of the best antibacterial effect and the best antidiarrhea, heat-clearing and damp-drying, astringent and hemostatic effect of the composition 4, which is compatible with Codonopsis pilosula, Poria cocos and Psoralea corylifolia, has achieved a better effect. In the process of exploring the dosage of Chinese medicine components, we found that the drug components in the Chinese medicine prescription remain unchanged, only the dosage of certain medicinal flavors is changed, so that the monarch-minister relationship of the Chinese medicinal flavors in the prescription changes, and the function and main treatment of the whole prescription will also change accordingly. Although different Chinese medicine compositions in the present invention have improved the diarrhea of ​​mice to a certain extent, the difference in the dosage of each component has also produced a more obvious effect on the drug effect. For the appropriate increase in the dosage of Galla chinensis, while increasing the dosage of drugs such as Codonopsis pilosula, Poria cocos, Psoralea corylifolia, etc. that have tonifying the middle qi and enhancing immunity, the drug effect can be improved to a certain extent (Chinese medicine composition 6), but too much increase (such as Chinese medicine composition 7) has not been able to further improve the overall efficacy of the prescription. The Chinese medicine composition of the present application is mainly used for treating bacterial diarrhea, with bacteria being extremely sensitive, astringent to stop diarrhea, clearing away heat, drying dampness and detoxifying, cooling blood and stopping diarrhea as the main, and then assisting in supporting the middle and replenishing qi, promoting dampness and strengthening the spleen, so as to achieve both suppressing and killing pathogens, and relieving the diarrhea symptoms caused by bacterial infection, while improving the body's resistance, and combining strengthening the body and removing evil. Reliable prescriptions should not only be targeted and just in the right condition, but also be rigorous in formulating, with clear priorities in medication, and good at clever compatibility. Therefore, Chinese medicine composition 6 of the present invention has unexpected effects.

[0084] Finally, it should be noted that the above is only an example of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned implementation, it is still possible for a person skilled in the art to modify the technical solution recorded in the aforementioned implementation, or to perform equivalent replacement of part thereof. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention. Although the above describes the specific implementation mode of the present invention, it is not intended to limit the protection scope of the present invention. A person skilled in the art should understand that, based on the technical solution of the present invention, various modifications or deformations that can be made by those skilled in the art without creative labor are still within the protection scope of the present invention.

Claims

1. A Chinese medicine composition for preventing or treating diarrhea in livestock and poultry, characterized in that: The invention comprises the following components in parts by weight: 10-20 parts of gallnut, 6-15 parts of scutellaria, 6-15 parts of euphorbia pulex, 4-10 parts of sophora japonica, 10-15 parts of codonopsis pilosula, 6-15 parts of tuckahoe, 5-10 parts of psoralea corylifolia and 3-5 parts of liquorice.

2. The Chinese medicine composition according to claim 1, characterized in that: The invention comprises the following components in parts by weight: 12-16 parts of gallnut, 6-10 parts of scutellaria, 6-10 parts of herba euphorbiae, 4-8 parts of sophora japonica, 12-15 parts of codonopsis pilosula, 10-13 parts of tuckahoe, 5-8 parts of psoralea corylifolia and 3-5 parts of liquorice.

3. The Chinese medicine composition according to claim 1, characterized in that The invention comprises the following components in parts by weight: 15 parts of gallnut, 8 parts of scutellaria, 8 parts of herba euphorbiae, 6 parts of sophora japonica, 12 parts of codonopsis pilosula, 12 parts of tuckahoe, 6 parts of psoralea corylifolia and 3 parts of liquorice.

4. The Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The dosage form of the Chinese medicine composition is any one of decoction, powder, granule, powder, pill or capsule.

5. The method for preparing the Chinese medicine composition according to any one of claims 1 to 3, characterized in that: The method comprises the following steps: weighing each component according to weight, soaking in water, then decocting twice, filtering and combining the medicinal liquid.

6. The preparation method according to claim 5, characterized in that: The water soaking is to add 8 to 10 times the total weight of the medicine in water and soak at 4 to 6° C. for 10 to 12 hours.

7. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of veterinary drugs, feed additives or feed for preventing or treating diarrhea.

8. The use according to claim 7, characterized in that: When preparing feed for preventing or treating diarrhea, the Chinese medicine composition is prepared into a common dosage form and added into the feed to obtain the medicated feed.

9. The use according to claim 8, characterized in that: The amount of the Chinese medicine composition added to the feed is 3-40 g / kg feed based on the complete feed.

10. The use according to claim 7, characterized in that: The application animals of the Chinese medicine composition are cattle, sheep or pigs.

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