Traditional Chinese medicine fermentation preparation for dispelling effects of alcohol and protecting liver and preparation method thereof

Abstract

The invention belongs to the technical field of biological medicine, and particularly relates to a traditional Chinese medicine fermentation preparation for dispelling effects of alcohol and protecting liver and a preparation method thereof. The preparation method of the traditional Chinese medicine fermented preparation for dispelling effects of alcohol and protecting liver comprises the following steps: (1) weighing the raw material medicines of cassia twig, davidsonia oblonga leaf, cynanchum mooreanum, cenchrus caltrop, gynostemma pentaphylla, ginseng, sterculia nobilis and liquorice, crushing, sieving, soaking with water, boiling with strong fire, decocting with slow fire, stopping fire, filtering, and concentrating the filtrate to obtain an extracting solution of the traditional Chinese medicine composition; and (2) inoculating the lactobacillus plantarum liquid and the lactobacillus rhamnosus liquid into the extracting solution of the traditional Chinese medicine composition, fermenting, centrifuging, filtering and sterilizing to obtain the traditional Chinese medicine composition. By taking the traditional Chinese medicine fermentation preparation prepared by the invention, the metabolism of alcohol can be accelerated, the damage of alcohol to the liver can be reduced, the traditional Chinese medicine fermentation preparation has obvious effects of dispelling the effects of alcohol and protecting the liver, and the traditional Chinese medicine fermentation preparation is green and non-toxic, conforms to food safety and has a wide application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine, and particularly relates to a traditional Chinese medicine fermentation preparation for sobering up and protecting the liver and a preparation method thereof. Background Art

[0002] Excessive drinking can cause pathological damage to liver cells such as edema and fatty degeneration. Long-term alcohol abuse can lead to chronic alcohol poisoning, fatty liver, hepatitis, cirrhosis, and eventually liver cancer. In recent years, alcoholic liver damage has received increasing attention from society due to excessive drinking. In order to alleviate this problem, the consumption of alcohol-relieving and liver-protecting products has continued to heat up. Some products that can effectively accelerate alcohol metabolism and protect the liver have broad market prospects because they play an important role in preventing and treating liver diseases caused by excessive drinking.

[0003] The hangover-relief products currently sold on the market are mainly chemical drugs and enzyme preparations. Chemical drugs mostly contain stimulant ingredients, which have limited effect on reducing the damage of alcohol to the liver, low hangover efficiency, and increase the detoxification burden of the liver and kidneys, accompanied by significant toxic side effects. Although enzyme preparations can quickly decompose acetaldehyde and absorb alcohol, their high price limits their widespread application.

[0004] Therefore, for the current hangover relieving product market, developing products with good taste, low price, low toxicity and side effects, and significant hangover relieving effect and liver protection function is an urgent problem to be solved. Summary of the invention

[0005] The first purpose of the present invention is to provide a method for preparing a fermented Chinese medicinal preparation for sobering up and protecting the liver.

[0006] The purpose of the present invention is achieved through the following technical solutions:

[0007] A method for preparing a traditional Chinese medicine fermented preparation for sobering up and protecting the liver comprises the following steps:

[0008] (1) Weigh 10-25 parts of cinnamon twig, 5-25 parts of Alsophila sibiricum leaf, 5-20 parts of Laojunxu, 5-15 parts of Tribulus terrestris, 2-15 parts of Gynostemma pentaphyllum, 5-15 parts of Ginseng, 2-10 parts of Eichhornia crassipes, and 5-10 parts of Licorice, grind and sieve, soak in water, boil over high heat, then simmer over low heat, filter after stopping the heat, and concentrate the filtrate to a density of 1-1.2 g / mL to obtain an extract of the traditional Chinese medicine composition;

[0009] (2) inoculating Lactobacillus plantarum and Lactobacillus rhamnosus into the extract of the Chinese medicine composition for fermentation, and then centrifuging, filtering and sterilizing to obtain the extract.

[0010] Furthermore, the weight proportions of the raw materials in step (1) are: 17 parts of cinnamon twig, 15 parts of Alsophila sibiricum leaf, 12 parts of Laojunxu, 10 parts of Tribulus terrestris, 8 parts of Gynostemma pentaphyllum, 10 parts of Ginseng, 6 parts of Eichhornia crassipes, and 7 parts of Licorice.

[0011] Furthermore, the number of viable bacteria in the Lactobacillus plantarum and Lactobacillus rhamnosus bacterial solutions in step (2) is 1.5×10 9 -2.5×10 9 CFU / g.

[0012] Furthermore, in step (2), the Lactobacillus plantarum liquid, the Lactobacillus rhamnosus liquid and the extract of the traditional Chinese medicine composition are inoculated at a volume ratio of 5:(5-8):100.

[0013] Furthermore, the fermentation conditions in step (2) are: fermentation under anaerobic conditions at 30-33°C for 70-80 hours.

[0014] Furthermore, the plant lactobacillus is plant lactobacillus BLCC2-0015, and the rhamnosus lactobacillus is rhamnosus lactobacillus grx10.

[0015] Furthermore, in the step (1), the amount of water added is 4-8 times the total weight of each raw material drug, and the soaking time in water is 60-120 minutes.

[0016] Furthermore, the decocting time on low heat in step (1) is 40-60 minutes.

[0017] Furthermore, the preparation process of the Lactobacillus plantarum and Lactobacillus rhamnosus bacterial liquids in step (2) is to inoculate Lactobacillus plantarum and Lactobacillus rhamnosus into MRS solid culture medium for screening and purification, and then inoculate into MRS liquid culture medium for culturing for 24-48 hours.

[0018] The second object of the present invention is to provide a traditional Chinese medicine fermentation preparation for sobering up and protecting the liver.

[0019] In order to achieve the above object, the technical solution adopted by the present invention is:

[0020] The alcohol-detoxifying and liver-protecting Chinese medicinal fermented preparation is prepared by the method for preparing the alcohol-detoxifying and liver-protecting Chinese medicinal fermented preparation described above.

[0021] In order to more easily understand the present invention, the effects of each raw material in the present invention are described here:

[0022] Cinnamon twig: pungent, sweet, warm in nature. It enters the bladder meridian, heart meridian, and lung meridian. Functions and indications: warms and dredges the meridians, helps yang and transforms qi, and balances and descends qi. The "Principles of Using Medicines" records: Cinnamon twig has a light smell and taste, so it can go up and disperse on the surface.

[0023] Alsophila sibiricum leaf: bitter, sweet, neutral, non-toxic. Enters the liver and lung meridians. Functions and indications: promote blood circulation, promote urination, reduce swelling, accelerate alcohol metabolism, and have the effect of sobering up. "Compendium of Materia Medica" records: stop bleeding, quench thirst, and promote urination.

[0024] Laojunxu: sweet and slightly spicy, neutral in nature. Enters the stomach and kidney meridians. Functions and indications: has the effects of tonifying the kidney, strengthening the spleen, and detoxifying. "Shaanxi Chinese Herbal Medicine" records: nourishes yin and tonifies the kidney, strengthens the spleen and replenishes qi, regulates menstruation and activates blood circulation.

[0025] Tribulus terrestris: bitter, pungent, neutral in nature. Enters the liver and lung meridians. Functions and indications: It has the effects of clearing away heat and detoxification, dispelling wind and relieving pain, calming the liver and suppressing yang, etc. It helps to relieve the discomfort symptoms such as heat and toxicity accumulation after drinking, sore throat, etc., while relieving liver pressure and promoting liver health. "Compendium of Materia Medica" records: It disperses liver wind and clears lung qi, overcomes dampness, cools blood and breaks blood.

[0026] Gynostemma pentaphyllum: bitter, slightly sweet, cool in nature. Enters the lung, spleen, and kidney meridians. Functions and indications: replenishing qi and strengthening the spleen, resolving phlegm and relieving cough, clearing away heat and detoxifying. The "Ming and Qing Dynasty Chinese Medicine Clinical Practice Series" records: replenishing qi and nourishing yin, clearing the lungs and resolving phlegm, nourishing the heart and calming the mind, and generating and consolidating essence.

[0027] Ginseng: sweet, slightly bitter, warm and neutral in nature. It enters the spleen, lung and heart meridians. Functions and indications: greatly replenishes vital energy, restores pulse and consolidates looseness, replenishes spleen and lungs, promotes fluid production and nourishes blood, calms the mind and improves intelligence. "Shennong's Herbal Classic" records: It mainly replenishes the five internal organs, calms the spirit, stabilizes the soul, stops panic, improves eyesight and improves intelligence, and long-term use can make the body lighter and prolong life.

[0028] Fengyan fruit: sweet, neutral in nature. Enters the stomach meridian, large intestine meridian, and small intestine meridian. Functions and indications: soothes the stomach and helps digestion, detoxifies and kills insects. "Compendium of Materia Medica" records: large ones, cooked and eaten, benefit the heart and spleen; eaten raw, quench thirst and produce body fluids.

[0029] Licorice: sweet, neutral in nature. It enters the heart, lung, spleen, and stomach meridians. It nourishes the spleen and replenishes qi, clears away heat and detoxifies, removes phlegm and relieves cough, relieves pain, and harmonizes various medicines. The Compendium of Materia Medica records that licorice is the king of all medicines, and can cure 72 kinds of milk stone poisons, detoxify 1,200 kinds of plant poisons, and has a great effect in harmonizing various medicines, so it is called "national elder".

[0030] Compared with the prior art, the beneficial effects of the present invention are mainly:

[0031] The alcohol-relieving and liver-protecting Chinese medicine fermentation preparation of the present invention is prepared by fermenting Chinese medicine extract with probiotics. In the Chinese medicine formula, cinnamon twig and Alsophila seu Radix Astragali are used as the main medicine, which has the effect of accelerating blood circulation and improving alcohol metabolism; Laojunxu and Tribulus terrestris are used as the ministerial medicine, which can strengthen the spleen and replenish qi, clear away heat and detoxify, and calm the liver and suppress yang. Gynostemma pentaphyllum, ginseng, and Eichhornia crenata are used as auxiliary medicines to refresh the mind, calm the nerves and improve intelligence, and relieve nausea after drinking. Licorice is used as the adjuvant to harmonize the medicines. The Chinese medicine formula of the present invention is reasonably compatible, has the functions of promoting ethanol metabolism, alcohol-relieving and liver-protecting, and is fermented with probiotics. The two mutually synergize and enhance the activity of the Chinese medicine of the preparation, and enhance the alcohol-relieving and liver-protecting effect. The test results show that by taking the Chinese medicine fermentation preparation prepared by the present invention, the metabolism of alcohol can be accelerated, the damage of alcohol to the liver can be reduced, and it has obvious alcohol-relieving and liver-protecting effects. Moreover, the Chinese medicine fermentation preparation of the present invention is green and non-toxic, meets food safety, and has a wide range of application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The results of ALT content detection in mouse serum;

[0033] Figure 2 This is the result of AST content test in mouse serum;

[0034] Figure 3 This is the result of GSH content detection in mouse liver tissue homogenate. DETAILED DESCRIPTION

[0035] The technical scheme of the present invention is further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0036] The preservation number of the plant lactobacillus BLCC2-0015 of the present invention is CCTCC NO: M2015126, and the preservation number of the rhamnosus lactobacillus grx10 is CGMCC NO: 2526.

[0037] Example 1

[0038] A method for preparing a traditional Chinese medicine fermented preparation for sobering up and protecting the liver comprises the following steps:

[0039] (1) Weigh 17 parts of cinnamon twig, 15 parts of Alsophila sibiricum leaf, 12 parts of Laojunxu, 10 parts of Tribulus terrestris, 8 parts of Gynostemma pentaphyllum, 10 parts of Ginseng, 6 parts of Eichhornia crassipes, and 7 parts of Licorice, grind and sieve, add 6 times the total weight of each raw material in water, soak for 90 minutes, boil over high heat until boiling, then simmer over low heat for 50 minutes, filter after stopping the heat, collect the filtrate, repeat the above soaking and decocting operations for the residue 3 times, combine the filtrate and concentrate to a density of 1.10 g / mL to obtain an extract of the traditional Chinese medicine composition;

[0040] (2) Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx10 were streaked onto MRS solid medium, cultured in an anaerobic chamber at 30°C for 24 h, and single colonies were picked and inoculated into MRS liquid medium at 30°C for 36 h to obtain bacterial liquids of Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx1. The number of viable cells of Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx1 was about 2.0×10 9 CFU / g;

[0041] (3) The bacterial liquid of Lactobacillus plantarum BLCC2-0015, the bacterial liquid of Lactobacillus rhamnosus grx10 and the extract of the Chinese medicine compound composition were inoculated at a volume ratio of 5:6:100. After the components were evenly stirred, they were fermented under anaerobic conditions at 31°C for 75 hours, centrifuged, filtered and sterilized to obtain a Chinese medicine fermented preparation for alcohol sobering and liver protection.

[0042] This embodiment also provides a traditional Chinese medicine fermented preparation for alcohol sobering up and protecting the liver obtained by the above preparation method.

[0043] Example 2

[0044] A method for preparing a traditional Chinese medicine fermented preparation for sobering up and protecting the liver comprises the following steps:

[0045] (1) Weigh 10 parts of cinnamon twig, 5 parts of Alsophila sibiricum leaf, 5 parts of Laojunxu, 5 parts of Tribulus terrestris, 2 parts of Gynostemma pentaphyllum, 5 parts of Ginseng, 2 parts of Eichhornia crassipes, and 5 parts of Licorice, grind and sieve, add 4 times the total weight of each raw material in water, soak for 60 minutes, boil over high heat until boiling, then simmer for 40 minutes over low heat, filter after stopping the heat, collect the filtrate, repeat the above soaking and decocting operations for the residue 3 times, combine the filtrate and concentrate to a density of 1.00 g / mL to obtain an extract of the traditional Chinese medicine composition;

[0046] (2) Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx10 were streaked onto MRS solid medium, cultured in an anaerobic chamber at 30°C for 24 h, and single colonies were picked and inoculated into MRS liquid medium and cultured at 30°C for 24 h to obtain Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx1 bacterial liquids, wherein the number of viable cells of Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx1 was approximately 1.5×10 9 CFU / g;

[0047] (3) The bacterial liquid of Lactobacillus plantarum BLCC2-0015, the bacterial liquid of Lactobacillus rhamnosus grx10 and the extract of the Chinese medicine compound composition were inoculated at a volume ratio of 5:5:100. After the components were evenly stirred, they were fermented under anaerobic conditions at 30°C for 70 hours, centrifuged, filtered and sterilized to obtain a Chinese medicine fermented preparation for alcohol sobering and liver protection.

[0048] This embodiment also provides a traditional Chinese medicine fermented preparation for alcohol sobering up and protecting the liver obtained by the above preparation method.

[0049] Example 3

[0050] A fermented Chinese medicinal preparation for sobering up and protecting the liver comprises the following raw materials in parts by weight: 25 parts of cassia twig, 25 parts of alsoensis leaves, 20 parts of laurel roots, 15 parts of tribulus terrestris, 15 parts of gynostemma pentaphyllum, 15 parts of ginseng, 10 parts of water chestnuts, and 10 parts of liquorice.

[0051] A method for preparing a traditional Chinese medicine fermented preparation for sobering up and protecting the liver comprises the following steps:

[0052] (1) Weigh 25 parts of cinnamon twig, 25 parts of Alsophila sibiricum leaf, 20 parts of Laojunxu, 15 parts of Tribulus terrestris, 15 parts of Gynostemma pentaphyllum, 15 parts of Ginseng, 10 parts of Eichhornia crassipes, and 10 parts of Licorice, grind and sieve, add 8 times the total weight of each raw material in water, soak for 120 minutes, boil over high heat until boiling, then simmer over low heat for 60 minutes, filter after stopping the heat, collect the filtrate, repeat the above soaking and decocting operations for the residue 3 times, combine the filtrate and concentrate to a density of 1.20 g / mL to obtain an extract of the traditional Chinese medicine composition;

[0053] (2) Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx10 were streaked onto MRS solid medium, cultured in an anaerobic chamber at 30°C for 24 h, and single colonies were picked and inoculated into MRS liquid medium at 30°C for 48 h to obtain Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx1 bacterial liquids, wherein the number of viable cells of Lactobacillus plantarum BLCC2-0015 and Lactobacillus rhamnosus grx1 was approximately 2.5×10 9 CFU / g;

[0054] (3) The bacterial liquid of Lactobacillus plantarum BLCC2-0015, the bacterial liquid of Lactobacillus rhamnosus grx10 and the extract of the Chinese medicine compound composition were inoculated at a volume ratio of 5:8:100, and the components were evenly stirred and fermented under anaerobic conditions at 33°C for 80 hours, centrifuged, filtered, and sterilized to obtain a Chinese medicine fermented preparation for alcohol sobering and liver protection.

[0055] This embodiment also provides a traditional Chinese medicine fermented preparation for alcohol sobering up and protecting the liver obtained by the above preparation method.

[0056] Comparative Example 1

[0057] The difference between Comparative Example 1 and Example 1 is that the Alsophila quinata leaves are omitted in step (1), and the rest are the same as Example 1.

[0058] Comparative Example 2

[0059] The difference between Comparative Example 2 and Example 1 is that Tribulus terrestris is omitted in step (1), and the rest is the same as Example 1.

[0060] Comparative Example 3

[0061] The difference between Comparative Example 3 and Example 1 is that the Alsophila sibiricum leaves are omitted in step (1), and the amount of cassia twig is adjusted to 32 parts.

[0062] Comparative Example 4

[0063] The difference between Comparative Example 4 and Example 1 is that in step (1), Tribulus terrestris is omitted and the amount of Laojunxu is adjusted to 22 parts.

[0064] Test Example 1

[0065] The Waller-Hoch method was used to determine the effect of the Chinese medicine fermentation preparation of the present invention on the activity of alcohol dehydrogenase in vitro. + There are maximum absorption peaks at 340nm and 260nm respectively. + When it is converted into NADH, the absorbance changes, and the change value is proportional to the rate of the enzymatic reaction. + The activity of alcohol dehydrogenase can be determined by the change of absorbance at 340 nm.

[0066] Take 1.5mL of sodium pyrophosphate buffer with a pH of 8.8, 1mL of 28mM NAD +Solution, 0.5mL 11.5% (v / v) ethanol were mixed evenly, 0.1mL of the Chinese medicine fermentation preparation prepared by Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4 were added respectively, 0.1mL of distilled water was added to the control group, and after 5min of 25°C water bath, 0.1mL of ethanol dehydrogenase (0.6μg / mL) solution was added and shaken. The absorbance at 340nm was measured, and recorded once every 10s, and the measurement was continued for 5min. The absorbance value (A340nm) was plotted against time, and the increase of △A340nm / 10s, i.e., the change of absorbance per unit time, was calculated. According to the molar extinction coefficient of NADH at 340nm being 6.22, the generation of NADH per unit time can be calculated, and then the enzyme activity of ethanol dehydrogenase is expressed. The activity of alcohol dehydrogenase is expressed as nanomoles of NADH generated per minute. Enzyme activity (nmol / min) = (ΔA340 / 10s) × (1 / 6.22) × (total reaction solution volume) × (dilution factor) × (60s / min). The results are shown in Table 1.

[0067] Table 1 Determination of in vitro alcohol dehydrogenase activity of fermented Chinese medicine preparations for alcohol relieving and liver protecting

[0068]

[0069]

[0070] As shown in Table 1, compared with the comparative examples 1-4, the alcohol-detoxifying and liver-protecting Chinese medicinal fermentation preparations prepared in Examples 1-3 of the present invention have higher in vitro alcohol dehydrogenase activity, indicating that the alcohol-detoxifying and liver-protecting Chinese medicinal fermentation preparations prepared from cinnamon twig, Alsophila sibiricum leaf, Laojunxu, Tribulus terrestris, Gynostemma pentaphyllum, ginseng, Eichhornia crassipes, licorice, and probiotics have the functions of efficiently promoting ethanol metabolism, detoxifying and protecting the liver.

[0071] Test Example 2

[0072] The experiment selected 100 C57BL / 6 male mice of 5-6 weeks, divided into blank group, model group, positive group, embodiment 1 group, embodiment 2 group, embodiment 3 group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group, 10 in each group. Adaptive feeding was performed for 3 days before the experiment. At 10 o'clock every morning, the blank group and the model group were given normal saline (0.2mL / 10g body weight), the positive group was gavaged with Neptune Jinzun (0.001g / 10g body weight, dissolved with 0.2mL normal saline), and the remaining experimental group mice were gavaged with the corresponding Chinese medicine fermentation preparation (0.2mL / 10g body weight) prepared by the present invention for one week. On the 7th day, each group of mice was gavaged with anhydrous ethanol (5g / kg body weight), and the blank group was injected with an equal amount of normal saline. After fasting for 12 days, mice were killed after anesthesia, blood was collected, and supernatant was taken at 5000rpm for 10min, and ALT and AST content in mouse serum were determined using a biochemical analyzer. The mice were dissected and the liver tissues were removed. The liver tissues were rinsed and weighed, and then cut into small pieces with scissors. The pieces were added to a homogenizer, and 10% liver homogenate was prepared with 9 times the volume of normal saline by weight of the liver tissue. The liver homogenate was placed in a centrifuge tube and centrifuged at 5000 rpm for 10 minutes. The supernatant was taken and the GSH content in the mouse liver tissue homogenate was determined.

[0073] like Figure 1 and Figure 2 The following are the results of ALT and AST test in mouse serum. ALT and AST levels are extremely important biochemical indicators, mainly used to evaluate liver function. Figure 1 and Figure 2 It can be seen that compared with the blank group, after the model group mice were gavaged with anhydrous ethanol, the ALT and AST levels in the serum increased significantly, and liver cells were damaged. After gavage of the Chinese medicine fermentation preparation of the present invention, compared with the comparative example 1-4 group, the alcohol-detoxifying and liver-protecting Chinese medicine fermentation preparations prepared by the examples 1-3 of the present invention can significantly reduce the ALT and AST levels in the serum of mice. This shows that the alcohol-detoxifying and liver-protecting Chinese medicine fermentation preparations prepared by the present invention can play a certain role in alleviating alcoholic liver damage by lowering the ALT and AST levels in the serum induced by alcohol.

[0074] like Figure 3 The following is the result of the GSH content test in mouse liver tissue homogenate. GSH is mainly involved in the metabolism of drug toxins. Figure 3 It can be seen that compared with the blank group, the GSH of the model group mice decreased significantly after being gavaged with anhydrous ethanol. After gavage with the Chinese medicine fermentation preparation of the present invention, compared with the comparative example 1-4 group, the alcohol-detoxifying and liver-protecting Chinese medicine fermentation preparations prepared in Example 1-3 of the present invention can significantly increase the GSH content in the liver tissue of mice. This shows that the alcohol-detoxifying and liver-protecting Chinese medicine fermentation preparations prepared by the present invention can enhance the liver's ability to resist lipid peroxidation by increasing the GSH content, and reduce the damage of alcohol and metabolites to the liver.

[0075] Further analysis shows that compared with Example 1, the Chinese medicine fermentation preparation component of Comparative Example 1 omits the leaf of Alsophila sutchuenensis, the Chinese medicine fermentation preparation component of Comparative Example 2 omits the grass of Tribulus terrestris, and the Chinese medicine fermentation preparation component of Comparative Example 3 omits the leaf of Alsophila sutchuenensis, and the dosage of Cinnamomum cassiae is adjusted to the sum of the two monarch drugs; the Chinese medicine fermentation preparation component of Comparative Example 4 omits the grass of Tribulus terrestris, and the dosage of Laojunxu is adjusted to the sum of the two minister drugs, and the alcohol-detoxifying and liver-protecting effects of the four groups of Chinese medicine fermentation preparations on mice are significantly reduced. It shows that the two monarch drugs of Cinnamomum cassiae and Alsophila sutchuenensis, and the two minister drugs of Junxu and Tribulus terrestris have a synergistic effect. With Cinnamomum cassiae and Alsophila sutchuenensis as monarch drugs, Junxu and Tribulus terrestris as minister drugs, Gynostemma pentaphyllum, Ginseng, and Eichhornia crassipes as adjuvant drugs, Licorice as a messenger drug, and all the drugs are used together, and the whole prescription can effectively promote ethanol metabolism and play a significant alcohol-detoxifying and liver-protecting effect.

[0076] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. A method for preparing a traditional Chinese medicine fermentation preparation for sobering up and protecting the liver, characterized in that: The following steps are involved: (1) Weigh 10-25 parts of cinnamon twig, 5-25 parts of Alsophila sibiricum leaf, 5-20 parts of Laojunxu, 5-15 parts of Tribulus terrestris, 2-15 parts of Gynostemma pentaphyllum, 5-15 parts of Ginseng, 2-10 parts of Eichhornia crassipes, and 5-10 parts of Licorice, grind and sieve, soak in water, boil over high heat, then simmer over low heat, filter after stopping the heat, and concentrate the filtrate to a density of 1-1.2 g / mL to obtain an extract of the traditional Chinese medicine composition; (2) inoculating Lactobacillus plantarum and Lactobacillus rhamnosus into the extract of the Chinese medicine composition for fermentation, and then centrifuging, filtering and sterilizing to obtain the extract.

2. The method for preparing the alcohol-relieving and liver-protecting Chinese medicinal fermentation preparation according to claim 1, characterized in that: The weight proportions of the raw materials in step (1) are: 17 parts of cinnamon twig, 15 parts of Alsophila sibiricum leaf, 12 parts of Laojunxu, 10 parts of Tribulus terrestris, 8 parts of Gynostemma pentaphyllum, 10 parts of Ginseng, 6 parts of Eichhornia crassipes, and 7 parts of Licorice.

3. The method for preparing the alcohol-relieving and liver-protecting Chinese medicinal fermentation preparation according to claim 1, characterized in that: The number of viable bacteria in the Lactobacillus plantarum and Lactobacillus rhamnosus bacterial solutions in step (2) is 1.5×10 9 -2.5×10 9 CFU / g.

4. The method for preparing the alcohol-relieving and liver-protecting Chinese medicinal fermentation preparation according to claim 1, characterized in that: In step (2), the Lactobacillus plantarum liquid, the Lactobacillus rhamnosus liquid and the extract of the Chinese medicine composition are inoculated at a volume ratio of 5:(5-8):

100.

5. The method for preparing the alcohol-relieving and liver-protecting Chinese medicinal fermentation preparation according to claim 1, characterized in that: The fermentation conditions in step (2) are: fermentation under anaerobic conditions at 30-33°C for 70-80 hours.

6. The method for preparing the alcohol-relieving and liver-protecting Chinese medicinal fermentation preparation according to claim 1, characterized in that: The plant lactobacillus is plant lactobacillus BLCC2-0015, and the rhamnosus lactobacillus is rhamnosus lactobacillus grx10.

7. The method for preparing the alcohol-relieving and liver-protecting Chinese medicinal fermentation preparation according to claim 1, characterized in that: In the step (1), the amount of water added is 4-8 times the total weight of each raw material drug, and the soaking time in water is 60-120 minutes.

8. The method for preparing the alcohol-relieving and liver-protecting Chinese medicinal fermentation preparation according to claim 1, characterized in that: The time for simmering over low heat in step (1) is 40-60 minutes.

9. The method for preparing the alcohol-relieving and liver-protecting Chinese medicinal fermentation preparation according to claim 1, characterized in that: The preparation process of the Lactobacillus plantarum and Lactobacillus rhamnosus bacterial liquids in step (2) is as follows: after inoculating Lactobacillus plantarum or Lactobacillus rhamnosus into MRS solid culture medium for screening and purification, inoculating into MRS liquid culture medium for culturing for 24-48 hours.

10. A fermented Chinese medicinal preparation for sobering up and protecting the liver is prepared according to the method for preparing the fermented Chinese medicinal preparation for sobering up and protecting the liver according to any one of claims 1 to 9.

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