Traditional Chinese medicine dry distillation preparation for treating respiratory tract infection of livestock and poultry and strengthening spleen and preparation method thereof

Abstract

The invention belongs to the technical field of veterinary traditional Chinese medicines, and discloses a traditional Chinese medicine dry distillation preparation for treating respiratory tract infection of livestock and poultry and having the effect of tonifying spleen, which is prepared from the following raw materials in parts by weight: 10-50 parts of azedarach, 10-50 parts of radix sophorae flavescentis, 10-50 parts of dark plum kernel, 10-50 parts of hawthorn kernel, 10-50 parts of astragalus membranaceus and 10-50 parts of bunge pricklyash leaf. The traditional Chinese medicine composition is less in toxic and side effects, free of accumulation, free of drug resistance, capable of clearing away heat and drying dampness, relieving cough and asthma and killing pathogenic microorganisms, the respiratory diseases of livestock and poultry are treated, recovery of the respiratory diseases is facilitated, appetite can be quickly recovered after the animal diseases are treated, the food consumption of animals after diseases is increased, and growth after the diseases is not affected.

Description

Technical Field

[0001] The invention belongs to the technical field of veterinary Chinese medicines, and relates to a Chinese medicine dry distillation preparation for treating livestock and poultry respiratory tract infection and having spleen-strengthening effects and a preparation method thereof. Background Art

[0002] Respiratory tract infections in livestock and poultry are mainly caused by pathogenic microorganisms and other reasons. They seriously affect the growth and development of animals, and sometimes even cause death, causing huge economic losses to healthy breeding. In traditional Chinese medicine, when the Wei Qi of livestock and poultry is insufficient and the disease resistance is reduced, the lung's function of spreading and transforming becomes weak, and it cannot be spread throughout the body, cannot protect the skin surface, and the function of defending against the invasion of external evils becomes weak, and the body shows symptoms such as the invasion of external evils. Pathogenic microorganisms are often distributed in the environment and in or on the body of animals. Under the condition of low individual immunity, they often cause respiratory infections through a large number of proliferation. Among them, upper respiratory tract infections are mostly common viruses, and lower respiratory tract infections are caused by bacteria, mycoplasma, chlamydia and other microorganisms. Respiratory diseases caused by pathogenic microorganisms are often fever symptoms. Individuals will have increased body temperature, accelerated heart rate, conjunctival mucosal bleeding, redness and swelling, and the growth and development of sick animals will be hindered, emaciation, and high mortality. Long-term use of antibiotics can cause bacterial resistance and drug residues. Therefore, the use of Chinese medicine preparations to treat respiratory diseases in livestock and poultry has become the preferred medication option. Currently, veterinary pharmaceutical companies also have relevant therapeutic water-extracted or alcohol-extracted oral liquids, powders, etc., but the therapeutic effects are not very ideal. Summary of the invention

[0003] The present invention aims at the technical problem that the efficacy of Chinese veterinary medicines for treating respiratory tract infections in livestock and poultry is not ideal, and provides a Chinese medicine dry distillation preparation for treating respiratory tract infections in livestock and poultry and having the effect of strengthening the spleen. The prescription has few toxic and side effects, is non-accumulative, does not produce drug resistance, can clear away heat and dampness, relieve cough and asthma, kill pathogenic microorganisms, achieves the treatment of respiratory diseases in livestock and poultry, and increases the feed intake of animals after illness.

[0004] To achieve the above object, the present invention adopts the following technical solutions:

[0005] On the one hand, the present invention provides a Chinese medicine dry distillation preparation for treating respiratory tract infections in livestock and poultry and having a spleen-strengthening effect, which is made from the dry distillation liquid of the following raw materials in parts by weight and additional auxiliary materials: 10 to 50 parts of chinaberry bark, 10 to 50 parts of sophora flavescens, 10 to 50 parts of ebony seeds, 10 to 50 parts of hawthorn seeds, 10 to 50 parts of astragalus and 10 to 50 parts of Zanthoxylum bungeanum leaves.

[0006] Preferably, the Chinese medicine dry distillation preparation of the present invention is prepared by dry distillation liquid of the following raw materials in parts by weight plus auxiliary materials: 10 parts of chinaberry bark, 10 parts of sophora flavescens, 30 parts of ebony seeds, 30 parts of hawthorn seeds, 10 parts of astragalus and 30 parts of Zanthoxylum leaves.

[0007] Preferably, the auxiliary materials are humectants, solubilizers and emulsifiers.

[0008] Preferably, the mass of the moisturizing agent is 10-50% of the mass of the dry distillation liquid, and the moisturizing agent is one of glycerin, lauric acid or lanolin.

[0009] Preferably, the mass of the thickener is 10-50% of the mass of the dry distillation liquid, and the solubilizer is one of ethanol and propylene glycol.

[0010] Preferably, the mass of the emulsifier is 10-50% of the mass of the dry distillate, and the emulsifier is one of polyvinyl alcohol, Tween 80 or Span 60.

[0011] On the other hand, the present invention provides a method for preparing a Chinese medicine dry distillation preparation for treating respiratory tract infection in livestock and poultry and having spleen-strengthening effect, comprising the following steps:

[0012] 1) Weigh the raw materials according to the above weight parts, crush the bark of Melia azedarach, Sophora flavescens, Prunus mume seeds, Hawthorn seeds, Astragalus membranaceus, and Zanthoxylum bungeanum leaves into 80-100 meshes respectively, and then mix them evenly;

[0013] 2) ultrafinely grinding the mixture obtained in step 1) to 300-500 mesh;

[0014] 3) putting the ultrafine powder obtained in step 2) into a microwave cracking furnace, setting the microwave frequency and extraction temperature, and collecting the condensed dry distillate;

[0015] 4) The dry distillation liquid obtained in step 3) is mixed with a moisturizer, a solubilizer and an emulsifier, and stirred uniformly, and the pH value is adjusted to 3.0-6.0 with an alkali solution to obtain a dry distillation preparation of traditional Chinese medicine.

[0016] Preferably, the microwave frequency in the present invention is 915 MHz to 5.8 GHz, and the extraction temperature is 150 to 300°C, preferably 240 to 270°C, and more preferably 270°C.

[0017] In the prescription of the present invention:

[0018] Melia bark: Toxic, generally used as an insecticide, its main functions are clearing away heat, drying dampness, killing insects and sterilizing. Melia bark can also dry dampness and relieve itching.

[0019] Sophora flavescens: bitter in taste, cold in nature, enters the liver, kidney, stomach, and large intestine meridians. Good at descending and draining, with the effects of clearing away heat, drying dampness, promoting diuresis, relieving jaundice, and expelling wind and killing insects. It is mainly used to treat fever, chest congestion caused by heat toxins, thirst, diarrhea caused by dampness and heat, blood in the stool, vaginal discharge and itching, jaundice, red and astringent urine, pruritus caused by urticaria, scabies, leprosy, and sores caused by dampness and toxins.

[0020] Black plum kernel: As a traditional Chinese medicine, it has many functions and effects such as strengthening the spleen and appetite, relieving cough and reducing phlegm, nourishing yin and reducing fire, and replenishing blood. It is mostly used to improve the digestive system, respiratory system, and inflammation in the body.

[0021] Hawthorn kernel: Hawthorn kernel is the seed of the wild hawthorn plant of the genus Crataegus in the Rosaceae family. It is mild in nature and bitter in taste. It enters the stomach and liver meridians. It has the effects of digesting food, dispersing knots, and inducing labor. It has a therapeutic effect on hernia, testicular prolapse, dystocia, indigestion, etc. It also has the effects of relieving stomach acid and abdominal distension and pain, lowering blood lipids, sterilizing, softening blood vessels, and preventing atherosclerosis. In addition, hawthorn kernel has a therapeutic effect on vaginitis, thrush, psoriasis, onychomycosis, athlete's foot and many other skin diseases.

[0022] Astragalus: Astragalus is sweet and warm in nature. It is a tonic medicine that is good at replenishing qi. Astragalus is a medicine that replenishes qi and consolidates the exterior. It is used for spontaneous sweating and fatigue caused by exterior deficiency. It is also used for edema caused by qi deficiency. Astragalus has the effects of diuresis, detoxification, sore astringence and tissue regeneration. It is used to strengthen the spleen and stomach. It is used for qi deficiency, lack of strength, less appetite, sinking of middle qi, long-term diarrhea and dysentery, rectal prolapse and bloody stool.

[0023] Zanthoxylum bungeanum leaves: pungent and warm, with functions of dispelling wind and dampness, promoting blood circulation and unblocking meridians, and strengthening the spleen and stomach. It is mainly used to treat wind-cold-dampness arthralgia, traumatic injury, yin deficiency, and skin itching. It contains a small amount of volatile oil and horse chestnut bark dimethyl ether, and the volatile oil contains limonene, linalool, and linalool acetate.

[0024] According to the Chinese medicine prescription, the formula of the present invention is as follows: Melia bark can clear away heat and dry dampness, and Sophora flavescens can clear away heat and dry dampness, promote diuresis and reduce phlegm. The two are combined as the main medicine to relieve lung heat; Plum kernel and Hawthorn kernel have the functions of relieving cough and reducing phlegm, nourishing yin and reducing fire, replenishing blood, digesting food and dispersing knots, strengthening spleen and stomach, killing bacteria and reducing inflammation, and promoting smooth muscle movement, and are the ministerial medicine; Astragalus can nourish qi, promote diuresis and remove toxins, and heal sores and promote tissue regeneration as the adjuvant medicine; At the same time, Astragalus and Zanthoxylum leaves can dispel cold and strengthen the spleen as the guiding medicine. All the medicines are used together to play the effects of clearing away heat and detoxification, resisting bacteria and reducing inflammation and swelling, nourishing spleen and promoting qi and dispersing knots.

[0025] Compared with conventional water extraction and alcohol extraction, the dry distillation extraction process adopted by the present invention has a better clinical effect than water extraction, alcohol extraction and powder. The six medicinal materials, namely, Melia azedarach bark, Sophora flavescens, Crataegus pinnatifida seed, Plum ebony seed, Astragalus membranaceus and Zanthoxylum bungeanum leaf, are subjected to anaerobic pyrolysis in the dry distillation extraction process under anaerobic and high temperature conditions, and new substances such as guaiacol are generated, which not only retains the effects of the original drugs, but also enhances the effects between compound drugs, and the new substances are more conducive to the recovery of respiratory diseases.

[0026] The present invention firstly grinds the medicinal material into coarse powder and then ultrafinely grinds it to obtain micron-grade powder material. The cell wall breaking rate of the medicinal material is greater than 95%, the surface area is increased, and the effective components are released faster, thereby increasing the extraction rate and extraction speed of subsequent dry distillation extraction. Alcohol is subsequently added to facilitate the dissolution of new substances therein, and glycerol and polyethylene 2000 are added to make the dry distillation liquid stable and not easy to precipitate, thereby reducing the waste of effective components.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] The prescription of the present invention has few toxic and side effects, is non-accumulative, does not produce drug resistance, can clear away heat and dampness, relieve cough and asthma, kill pathogenic microorganisms, achieves the effects of treating respiratory diseases in livestock and poultry and strengthening the spleen, is more conducive to the recovery of respiratory diseases, can quickly restore the appetite of animals after the treatment of diseases, increase the feed intake of animals after the disease, and does not affect the growth after the disease.

[0029] The invention is a pure Chinese medicine preparation, which can replace antibiotics and has a relatively ideal therapeutic effect. It avoids the phenomenon of destroying the microecological system of the animal body after antibiotic treatment, which easily causes stiff cows, stiff pigs, etc. DETAILED DESCRIPTION

[0030] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means known to those skilled in the art. The test methods in the following examples are conventional methods unless otherwise specified.

[0031] The model of the pulverizer used in the following examples is: JYNU40-37CS; the model of the ultrafine pulverizer is: WFM-100; the model of the microwave cracking furnace is: LM-SD-15. The moisture content of the following medicinal materials is controlled within 8%. The concentration of ethanol is 95%. Polyvinyl alcohol is polyvinyl alcohol 2000, purchased from Jinan Ruihongde Chemical Technology Co., Ltd. Glycerin is medical grade glycerin, purchased from Shandong Hengke Biotechnology Co., Ltd.

[0032] Example 1

[0033] Compound formula and weight: 1000g of Melia bark, 1000g of Sophora flavescens, 1000g of Plum kernel, 1000g of Hawthorn kernel, 1000g of Astragalus, and 1000g of Zanthoxylum leaf.

[0034] The above compound formula was prepared into test samples by four methods: water extraction, alcohol extraction, dry distillation extraction, and ultrafine grinding. Animal experiments were carried out. At the same time, it was compared with antibiotics, the effects were statistically analyzed, and the guaiacol content was detected.

[0035] 1) Alcohol extraction group: firstly grind the powder into 100 mesh with a pulverizer, then perform conventional alcohol extraction (5 times ethanol, 2 extractions, 1.5 h each time), and concentrate 1:1, i.e. 1 mL is equivalent to 1 g of the original medicinal material, to obtain the alcohol extraction infusion solution.

[0036] 2) Water extraction group: first use a grinder to grind the powder into 100 mesh, then use conventional water extraction (8 times water, extraction twice, each time 1.5 h), and concentrate 1:1, that is, 1 mL is equivalent to 1 g of the original medicinal material, to obtain the water extraction infusion solution.

[0037] 3) Dry distillation extraction group: First, use a pulverizer to coarsely grind to 100 mesh, then use ultrafine powder to grind to 500 mesh, and then use a microwave cracking furnace for dry distillation extraction, set the microwave frequency to 2.45GHz, set the temperature to 270℃, and collect the condensed dry distillate. The dry distillate is mixed with ethanol, glycerol, and polyvinyl alcohol 2000 in a mass ratio of 3:3:3:1 to prepare a dry distillation perfusion solution, which is converted to 1g of the original medicinal material per 1ml.

[0038] 4) Ultrafine powder oral group: first use a grinder to coarsely grind to 100 mesh, and then use an ultrafine grinder to ultrafinely grind to 500 mesh for oral administration with drinking water.

[0039] 5) Antibiotics (10% tylosin + 10% sulfamethoxazole): Mix with drinking water: 2-4g per liter of water for chickens, for 5 consecutive days.

[0040] The above five drugs were tested for pharmacodynamics on chickens, an experimental model animal of respiratory tract disease infection. 180 20-day-old roosters were randomly divided into 6 groups, 30 in each group. According to their weight and dosage, the drugs in each group were added to the drinking fountains of each group, divided into: water extraction group (0.6 ml / chicken / day, for 5 consecutive days), alcohol extraction group (0.6 ml / chicken / day, for 5 consecutive days), dry distillation group (0.6 ml / chicken / day, for 5 consecutive days), superfine powder oral group (0.6 ml / chicken / day, for 5 consecutive days), antibiotic control group (10% tylosin + 10% sulfadimethoxine): calculated as this product, mixed drinking, 2-4 g per liter of water chicken, for 5 consecutive days. Blank control group (normal diet), normal diet of other experimental groups were added with corresponding drugs in drinking water, and the cure rate and mortality rate were statistically calculated after 5 days of treatment. Uncured judgment criteria: sick chickens will show obvious symptoms such as coughing, runny nose and sneezing, and the eyes and nasal cavity will secrete a large amount of secretions, frequently shake the nose, and breathe with the mouth open. As the disease progresses, the eyes of sick chickens swell and become congested, and dissection of chickens shows obvious catarrhal inflammation in the lungs, upper respiratory tract and air sacs of sick chickens, inflammatory edema in the eyes and head, and swelling of the lungs accompanied by congestion. Cure evaluation criteria: no clinically obvious respiratory disease symptoms (such as coughing, runny nose, sneezing, etc.), no secretions in the nasal cavity, the chickens are active and active, there is no obvious catarrhal inflammation in the trachea during dissection, and no obvious pulmonary edema in the lungs. The results are shown in Table 1.

[0041] Table 1 The therapeutic effects of different treatment groups on animals with respiratory diseases

[0042] Group Cure rate, % Uncured rate, % mortality rate,% Water extraction group 26.7 33.3 40.0 Alcohol extraction group 40.0 33.3 26.7 Dry distillation group 83.3 10.0 6.7 Ultrafine powder oral group 43.3 33.3 23.4 Antibiotic positive control group 80.0 16.7 3.3 Blank control group 6.7 53.3 40.0

[0043] From the results in Table 1, it can be seen that the dry distillate oral liquid group has the most ideal therapeutic effect on chicken respiratory diseases.

[0044] The effective ingredient (guaiacol) of the samples obtained by water extraction, alcohol extraction, dry distillation extraction and ultrafine grinding was detected by gas chromatography. The results are shown in Table 2.

[0045] Table 2 Comparison of guaiacol content results

[0046] Group Guaiacol content (mg / mL) Water extraction group 0.2 Alcohol extraction group 0.6 Dry distillation group 2.1 Ultrafine powder oral group 0.5

[0047] As can be seen from Table 2, the content of guaiacol in the dry distillation extraction oral liquid is the highest, which shows that compared with water extraction, alcohol extraction and ultrafine grinding alone, dry distillation extraction can significantly increase the content of guaiacol. Because guaiacol has the effects of promoting smooth muscle movement and relieving asthma and cough, combined with the treatment results in Table 1, the therapeutic effect of chicken respiratory diseases is closely related to the content of guaiacol. Therefore, it is necessary to screen the ratio of the formula based on the guaiacol content.

[0048] In order to obtain a more suitable dry distillation extraction temperature of the microwave cracking furnace, 500g of Sophora flavescens (moisture content controlled within 8%) was used as the medicinal material, firstly coarsely ground to 100 mesh by a pulverizer, then ultrafinely ground to 500 mesh, and then dry distilled and extracted by a microwave cracking furnace, the microwave frequency was set to 2.45GHz, and the temperature in the microwave cracking furnace was set to 150℃, 180℃, 210℃, 240℃, 270℃, and 300℃ for extraction, and the condensed dry distillate was collected. The extraction rate of the dry distillate and the guaiacol content were measured, as shown in Table 3.

[0049] Table 3 Different temperature distillation results

[0050]

[0051] Note: Extraction rate = volume of extracted distillate (ml) / weight of feed (g)*100%.

[0052] As shown in Table 3, with the increase of the dry distillation temperature, the extraction rate of the dry distillation liquid and the content of guaiacol also increase accordingly. However, after the temperature exceeds 270°C, the extraction rate of the dry distillation liquid and the content of guaiacol show a downward trend. Therefore, the optimal temperature range for dry distillation extraction is 240-270°C.

[0053] Example 2

[0054] The components and contents of the compound formula are as follows:

[0055] Compound formula 1: Melia azedarach bark 100g, Sophora flavescens 100g;

[0056] Compound formula 2: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 100g;

[0057] Compound formula 3: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 100g, Hawthorn kernel 100g;

[0058] Compound formula 4: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 100g, Hawthorn kernel 100g, Astragalus 100g;

[0059] Compound formula 5: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 100g, Hawthorn kernel 100g, Astragalus 100g, Zanthoxylum bungeanum leaf 100g;

[0060] Compound formula 6: Melia azedarach bark 200g, Sophora flavescens 200g, Plum kernel 100g, Hawthorn kernel 100g, Astragalus 100g, Zanthoxylum bungeanum leaf 100g;

[0061] Compound formula 7: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 200g, Hawthorn kernel 200g, Astragalus 100g, Zanthoxylum bungeanum leaf 100g;

[0062] Compound formula 8: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 100g, Hawthorn kernel 100g, Astragalus 200g, Zanthoxylum bungeanum leaf 200g;

[0063] Compound formula 9: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 300g, Hawthorn kernel 100g, Astragalus 100g, Zanthoxylum bungeanum leaf 100g;

[0064] Compound formula 10: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 100g, Hawthorn kernel 300g, Astragalus 100g, Zanthoxylum bungeanum leaf 100g;

[0065] Compound formula 11: Melia azedarach bark 100g, Sophora flavescens 100g, Plum kernel 300g, Hawthorn kernel 300g, Astragalus 100g, Zanthoxylum bungeanum leaf 100g;

[0066] Compound formula 12: 100g of Melia bark, 100g of Sophora flavescens, 300g of ebony kernel, 300g of hawthorn kernel, 100g of Astragalus, and 300g of Zanthoxylum bungeanum leaf.

[0067] 100 g each of Melia bark, Sophora flavescens, Plum kernel, Hawthorn kernel, Astragalus, and Zanthoxylum leaves, as well as compound formulas 1 to 12, are subjected to dry distillation treatment according to the following steps:

[0068] (1) According to the single drug and each compound formula, grind them into coarse powder to 100 mesh using a pulverizer, and then mix them evenly in a mixer;

[0069] (2) crushing the medicinal material powder obtained in step (1) into 500 meshes using an ultrafine grinder;

[0070] (3) The ultrafine powder mixture obtained in step (2) is put into a microwave cracking furnace, the microwave frequency is set to 2.45 GHz, the cracking temperature is set to 270° C., and the condensed distillate is collected;

[0071] (4) The dry distillate obtained in (3) is mixed with alcohol, glycerol and polyvinyl alcohol 2000 in a mass ratio of 3:3:3:1 and stirred to prepare a dry distillation perfusion solution. The pH value is adjusted to 5.8 with 0.5 mol / L sodium hydroxide solution.

[0072] The dry distillate samples obtained in step (3) were subjected to gas chromatography detection, with guaiacol as the reference substance. The detection results are shown in Table 4.

[0073] Table 4 Guaiacol content in single-drug and compound formula dry distillation samples

[0074] Medicinal materials / compound formula Content, mg / mL Medicinal materials / compound formula Content, mg / mL Neem bark 0.3 Sophora flavescens 0.6 Plum kernel 2.2 Hawthorn kernel 1.6 Astragalus 0.2 Sichuan pepper leaves 0.9 Compound formula 1 0.6 Compound formula 2 1.0 Compound formula 3 1.2 Compound formula 4 1.1 Compound formula 5 1.5 Compound formula 6 1.1 Compound formula 7 1.7 Compound formula 8 1.7 Compound formula 9 1.8 Compound formula 10 1.7 Compound formula 11 2.0 Compound formula 12 2.2

[0075] As shown in Table 4, the guaiacol content in ebony kernel was the highest among all single drugs, followed by hawthorn kernel and then Zanthoxylum leaf. Compared with compound formula 1 to compound formula 5, compound formula 5 had a higher content, which may be related to the diversity of Chinese medicine components. Compared with compound formula 6, compound formula 7 and compound formula 8, compound formula 5 had a higher guaiacol content of 1.7 mg / mL in compound formula 7 and compound formula 8. It can be inferred that Zanthoxylum leaf, ebony kernel and hawthorn kernel had a greater influence on the guaiacol content in the formula. The guaiacol contents in compound formula 7, compound formula 8, compound formula 9 and compound formula 10 were similar. The formula and proportion of compound formula 11 and compound formula 12 can better explain that the proportion of Zanthoxylum leaf, ebony kernel and hawthorn kernel increased, and the guaiacol content increased.

[0076] Example 3

[0077] A ranch was selected, and 240 Simmental beef cattle were transported from Northeast China to Zibo, Shandong Province for fattening in July 2024. Due to the hot weather, transportation stress and rain on the way, the cattle showed clinical symptoms of respiratory disease infection such as dyspnea, rapid heartbeat, runny nose, fever and anorexia within 3 days after landing. The Chinese medicine dry distillation preparation test product extracted by dry distillation of the compound formula 12 in Example 2 was used for clinical trials and compared with the commercially available lincomycin injection (30% content). Cured: no obvious clinical respiratory symptoms; effective: including cured and improved cattle; invalid: no obvious relief of the condition, requiring fluid infusion and further treatment. The treatment results are shown in Table 5.

[0078] Table 5 Treatment results for cattle infected with respiratory diseases

[0079]

[0080] As shown in Table 5, the dry distillation preparation prepared by compound formula 12 is more effective than the commonly used antibiotics on the market in treating respiratory diseases in beef cattle, and its cure rate and effective rate are higher than those of the antibiotic lincomycin control group.

[0081] Example 4

[0082] In a fattening pig farm, due to excessive stocking density and poor ventilation in March 2024, a fattening pig (weight 90-100kg) developed respiratory diseases. The Chinese medicine dry distillation preparation test product extracted by dry distillation of the compound formula 12 in Example 2 was used for clinical trials and compared with the enrofloxacin injection (5% content) on the market. At the same time, the intestinal digestion (spleen and stomach) feed intake index of pigs infected with respiratory diseases was statistically analyzed, and the results are shown in Table 6.

[0083] Table 6 Treatment results for pigs infected with respiratory diseases

[0084]

[0085]

[0086] As can be seen from Table 6, the therapeutic effect of the dry distillation preparation prepared by compound formula 12 on respiratory diseases of fattening pigs is better than that of enrofloxacin injection, an antibiotic commonly used on the market; at the same time, the feed intake of the enrofloxacin injection control group was significantly lower than that of the dry distillation oral liquid test group (low dose, high dose), which shows that the compound formula 12 of the present invention has the effect of strengthening the spleen and aiding digestion.

[0087] The embodiments described above are only preferred embodiments of the present invention and are only used to explain the present invention, not to limit the scope of implementation of the present invention. For those skilled in the art, other implementation methods can certainly be easily made by replacement or modification based on the technical contents disclosed in this specification. Therefore, all changes and improvements made on the principles of the present invention should be included in the scope of the patent application of the present invention.

Claims

1. A Chinese medicine dry distillation preparation for treating respiratory tract infections in livestock and poultry and having spleen-strengthening effects, characterized in that: The invention is prepared from the dry distillation liquid of the following raw materials in parts by weight and auxiliary materials: 10-50 parts of chinaberry bark, 10-50 parts of sophora flavescens, 10-50 parts of black plum kernel, 10-50 parts of hawthorn kernel, 10-50 parts of astragalus and 10-50 parts of Zanthoxylum bungeanum leaves.

2. The Chinese medicine dry distillation preparation according to claim 1, characterized in that: The invention is prepared from the dry distillation liquid of the following raw materials in parts by weight and auxiliary materials: 10 parts of chinaberry bark, 10 parts of sophora flavescens, 30 parts of black plum seeds, 30 parts of hawthorn seeds, 10 parts of astragalus and 30 parts of Zanthoxylum bungeanum leaves.

3. The Chinese medicine dry distillation preparation according to claim 1, characterized in that: The auxiliary materials are moisturizer, solubilizer and emulsifier.

4. The Chinese medicine dry distillation preparation according to claim 3, characterized in that: The mass of the moisturizing agent is 10-50% of the mass of the dry distillation liquid, and the moisturizing agent is one of glycerin, lauric acid or lanolin.

5. The Chinese medicine dry distillation preparation according to claim 3, characterized in that: The mass of the solubilizer is 10-50% of the mass of the dry distillation liquid, and the solubilizer is one of ethanol and propylene glycol.

6. The Chinese medicine dry distillation preparation according to claim 3, characterized in that: The mass of the emulsifier is 10-50% of the mass of the dry distillation liquid, and the emulsifier is one of polyvinyl alcohol, Tween 80 or Span 60.

7. The method for preparing the Chinese medicine dry distillation preparation according to any one of claims 1 to 6, characterized in that: The following steps are involved: 1) Weigh the raw materials according to the weight proportions described in claims 1 to 6, crush the bark of Melia azedarach, Sophora flavescens, Prunus mume seeds, Hawthorn seeds, Astragalus membranaceus, and Zanthoxylum bungeanum leaves into 80-100 meshes respectively, and then mix them evenly; 2) ultrafinely grinding the mixture obtained in step 1) to 300-500 mesh; 3) putting the ultrafine powder obtained in step 2) into a microwave cracking furnace, setting the microwave frequency and extraction temperature, and collecting the condensed dry distillate; 4) The dry distillation liquid obtained in step 3) is mixed with a moisturizer, a solubilizer, and an emulsifier, and stirred evenly, and the pH value is adjusted to 3.0-6.0 with an alkali solution to obtain a traditional Chinese medicine dry distillation preparation.

8. The preparation method according to claim 7, characterized in that: The microwave frequency is 915 MHz to 5.8 GHz, and the extraction temperature is 150 to 300° C.

Images