Fermented traditional Chinese medicine enzyme and preparation method thereof
Through specific Chinese medicine extracts and bacterial strain formulas, combined with ultrasonic extraction and multiple fermentation steps, traditional Chinese medicine enzymes with stable quality and sufficient active active ingredients are prepared, solving the problems of instability and insufficient activity of existing Chinese medicine enzymes in health care effects, and achieving improvements in microcirculation, anti-fatigue, anti-oxidation and other health care effects.
Patent Information
- Application Number
- CN202311497210.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-11-10
- Publication Date
- 2025-05-13
AI Technical Summary
The existing traditional Chinese medicine enzymes have problems of unstable quality and insufficient activity of active ingredients in terms of health care effects.
Using specific Chinese medicine extracts and strain formulas, Chinese medicine enzymes containing Lu's junction yeast, Aspergillus rosy and Lactobacillus acidophilus were prepared through ultrasonic extraction and multiple fermentation steps. The method includes multiple extraction and fermentation steps to ensure the stability of the enzyme and the maximum retention of the active ingredients.
It has achieved stable quality of traditional Chinese medicine enzymes, retained the activity and function of traditional Chinese medicine extracts to the maximum extent, and has the effects of improving microcirculation, anti-fatigue, anti-oxidation, delaying aging and strengthening the body.
Abstract
Description
Technical Field
[0001] The first object of the present invention is to provide a traditional Chinese medicine enzyme with excellent health care effects.
[0002] Another object of the present invention is to provide a method for preparing the traditional Chinese medicine enzyme.
[0003] In order to achieve the purpose of the present invention, the present invention adopts the following technical scheme: A Chinese medicine enzyme, characterized in that it is made of the following components in parts by weight: The Chinese medicine extract is made from the following raw materials in parts by weight: Preferably, the Chinese medicine enzyme can also be made of the following components in parts by weight: Preferably, the Chinese medicine extract can also be made from the following raw materials in parts by weight: The bacterial liquid contains 1 to 100 parts by weight of water and 1 to 10 parts by weight of bacterial strains.
[0004] The bacterial strain contains 1 to 20 parts by weight of Zygosaccharomyces rouxii, 1 to 25 parts by weight of Monascus purpureus and 1 to 30 parts by weight of Lactobacillus acidophilus.
[0005] The carbon source auxiliary material consists of 30 to 50 parts by weight of lactose, 20 to 60 parts by weight of honey and 10 to 50 parts by weight of sodium alginate.
[0006] A method for preparing a traditional Chinese medicine enzyme, characterized in that it comprises the following steps: a. Chinese herbal medicine extract (1) Weighing codonopsis pilosula, astragalus, apple, xylitol, and yam according to the weight of the formula, mixing with water at a weight ratio of 1:5 to 200, stirring and soaking, ultrasonic extraction, the time is 10 to 40 minutes, the ultrasonic power is 200 to 500W, the temperature is 50 to 80°C, the extracted mixture is heated to boiling, and filtered to obtain a first filtrate and a filter residue; (2) mixing the filter residue with water in a weight ratio of 1:5 to 200, performing ultrasonic extraction for 10 to 40 minutes, a power of 200 to 500 W, and a temperature of 50 to 80° C., heating the extracted mixture to boiling, and filtering to obtain a second filtrate and filter residue; (3) combining the first filtrate and the second filtrate, and centrifuging to obtain a Chinese medicine extract; b. First fermentation Weigh honey and sodium alginate in the carbon source auxiliary materials according to the weight of the formula, add them to the traditional Chinese medicine extract and mix them evenly, then add Zygosaccharomyces rouxii and Monascus purpureus according to the dosage of the formula, and ferment for 5 to 10 days under the conditions of temperature of 25 to 35° C., oxygen supply of 250 to 300 l / min, osmotic pressure of 0.07 to 0.08 MPa, and pH value of 3 to 4 to obtain the first fermentation liquid; c. Second fermentation Adding lactose and Lactobacillus acidophilus according to the formula amount to the first fermentation liquid, fermenting for 5 to 10 days under the conditions of temperature of 30 to 40° C., oxygen supply of 0.5 to 1.5 l / min, osmotic pressure of 0.07 to 0.08 MPa, and pH value of 2 to 4 to obtain a second fermentation liquid; d. Fermentation and maturation The temperature is kept constant at 38-40°C, and the fermentation is continued for 100-180 days to obtain the total fermentation liquid.
[0007] Finally, the total fermentation liquid is ultrasonically concentrated at low temperature, 1 to 20 parts by weight of vinegar and glucose are added for seasoning, and filtered to obtain the enzyme stock solution; or it can be dried at low temperature into powder as needed.
[0008] It is worth noting that the weight percentages of the bacterial strains and water involved in the present invention are set when the bacterial liquid is taken as a whole, and the weight percentages of Zygosaccharomyces rouxii, Monascus purpureus and Lactobacillus acidophilus involved in the present invention are set when the bacterial strains are taken as a whole. Those skilled in the art can also add other components on the basis of the formula involved in the present invention.
[0009] The components and strains used in the present invention are all commercially available. Experimental methods without specific conditions specified in the present invention are usually carried out under conventional conditions or conditions recommended by the manufacturer.
[0010] Compared with the prior art, the beneficial effects of the present invention are: stable quality, retaining the activity and function of the effective ingredients of the traditional Chinese medicine extract to the maximum extent, improving microcirculation, neutralizing excess free radicals in the human body, and having the effects of anti-fatigue, anti-oxidation, anti-aging, and strengthening the body. Background Art
[0011] The present invention relates to the field of health food, and specifically to a fermented Chinese medicine enzyme, which is a functional microbial fermentation product obtained by fermenting one or more fresh codonopsis, astragalus, apple, xylitol, yam, etc. with multiple beneficial bacteria. The enzyme not only preserves the nutrients inherent in the fermentation raw materials, but also produces new biologically active ingredients and biological enzymes, and has a strong ability to scavenge superoxide anion free radicals, hydroxyl free radicals, and dpph free radicals, and can expel toxins, balance the acid and alkali in the body, and then repair cells and adjust the body. Enzymes can generally be divided into six categories: acidification reductase, transfer enzyme, hydrolysis enzyme, separation enzyme, heterozygous enzyme and synthesis enzyme.
[0012] With the development of life sciences and the improvement of people's living standards, natural medicinal plant products have gradually gained favor among people, and the demand for natural medicinal plants has gradually developed in the direction of high efficiency and low toxicity. Therefore, it is necessary to develop more new microbial enzyme products using natural medicinal plants as raw materials to meet market demand. Summary of the invention
[0013] A Chinese medicinal enzyme is prepared from the following components in parts by weight: The Chinese medicine extract is made from the following raw materials in parts by weight: The bacterial liquid contains 50 parts by weight of water and 10 parts by weight of bacterial strains.
[0014] The bacterial strain contains 20 parts by weight of Zygosaccharomyces rouxii, 25 parts by weight of Monascus purpureus and 30 parts by weight of Lactobacillus acidophilus.
[0015] The carbon source auxiliary material consists of 30 parts by weight of lactose, 20 parts by weight of honey and 50 parts by weight of sodium alginate.
[0016] The preparation method of the traditional Chinese medicine enzyme comprises the following steps: a. Chinese herbal medicine extract (1) Weighing codonopsis pilosula, astragalus, apple, xylitol, and yam according to the weight of the formula, crushing them into 50 meshes respectively, then mixing them evenly, mixing them with water at a weight ratio of 1:100, stirring and soaking, and ultrasonic extraction for 10 minutes, with an ultrasonic power of 500w and a temperature of 60°C. The extracted mixture is heated to boiling and filtered to obtain a first filtrate and a filter residue; (2) mixing the filter residue with water in a weight ratio of 1:100, performing ultrasonic extraction for 10 min, a power of 500 W, and a temperature of 60° C., heating the extracted mixture to boiling, and filtering to obtain a second filtrate and filter residue; (3) combining the first filtrate and the second filtrate, and centrifuging to obtain a Chinese medicine extract; b. First fermentation Weigh honey and sodium alginate in the carbon source auxiliary materials according to the weight of the formula, add them to the traditional Chinese medicine extract and mix them evenly, then add Zygosaccharomyces rouxii and Monascus purpureus according to the dosage of the formula, and ferment for 8 days under the conditions of temperature of 25° C., oxygen supply of 250 l / min, osmotic pressure of 0.08 MPa, and pH value of 3-4 to obtain the first fermentation liquid; c. Second fermentation Adding lactose and Lactobacillus acidophilus according to the formula amount to the first fermentation liquid, fermenting for 6 days under the conditions of temperature 30°C, oxygen supply 0.5l / min, osmotic pressure 0.07mpa, and pH value 2-4 to obtain the second fermentation liquid; d. Fermentation and maturation The temperature of the second fermentation liquid is kept constant at 40°C, and the fermentation is matured for 100 days to obtain the total fermentation liquid. Finally, the total fermentation liquid is ultrasonically low-temperature concentrated, 15 parts by weight of vinegar and glucose are added for seasoning, and filtered to obtain the enzyme stock solution.
Claims
1. A Chinese medicine enzyme with excellent health care effects. A Chinese medicine enzyme, made of the following components in parts by weight: The Chinese medicine extract can also be made of the following raw materials in parts by weight: The weight ratio of the Chinese medicine extract, bacterial liquid, carbon source auxiliary material, and water is 10:1:1:
4. The bacterial liquid contains 30 parts by weight of water and 5 parts by weight of bacterial strains. The bacterial strain contains 15 parts by weight of Zygosaccharomyces rouxii, 10 parts by weight of Monascus, and 10 parts by weight of Lactobacillus acidophilus. The carbon source auxiliary material consists of 40 parts by weight of lactose, 30 parts by weight of honey, and 30 parts by weight of sodium alginate.
2. A preparation method of Chinese medicine enzyme. (1) Weigh codonopsis, astragalus, apple, xylitol, and yam according to the weight ratio of the formula, crush them into 70 meshes respectively, then mix them evenly, mix them with water at a weight ratio of 1:150, stir and soak, ultrasonically extract for 30 minutes, power of 300w, temperature of 60°C, heat the extracted mixture to boiling, filter, and obtain a first filtrate and filter residue; (2) Mix the filter residue with water at a weight ratio of 1:150, ultrasonically extract for 30 minutes, power of 300w, temperature of 60°C, heat the extracted mixture to boiling, filter, and obtain a second filtrate and filter residue; (3) Combine the first filtrate and the second filtrate, centrifuge and separate, and obtain a Chinese medicine extract.
3. The present invention relates to a Chinese medicine enzyme, which is made of the following components in parts by weight: 100-300 parts of Chinese medicine extract, 10-30 parts of bacterial liquid, 10-20 parts of carbon source auxiliary materials, and 50-100 parts of water; the Chinese medicine extract is made of the following raw materials in parts by weight: 30-50 parts of fruit, 30-60 parts of Codonopsis pilosula, 10-20 parts of Astragalus, 50-80 parts of apples, 20-50 parts of Chinese yam, and 10-15 parts of xylitol. The present invention also relates to a preparation method of the Chinese medicine enzyme. The present invention has stable quality, retains the activity and function of the effective ingredients of the Chinese medicine extract to the maximum extent, can improve microcirculation, neutralize excess free radicals in the human body, and has the effects of anti-fatigue, anti-oxidation, delaying aging, and strengthening the body.