Epimedium herb fermented oral thick paste for improving male renal function and preparation method thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine preparations, and particularly discloses herba epimedii whole herb fermented oral thick paste for improving male renal function and a preparation method thereof. The fermented oral thick paste is prepared from the following raw materials in parts by weight: 900 to 1200 parts of herba epimedii, 600 to 800 parts of black rice, 80 to 120 parts of black beans, 80 to 120 parts of fructus psoraleae, 40 to 60 parts of Chinese chive seeds, 150 to 200 parts of folium cortex eucommiae, 40 to 60 parts of salted semen cuscutae, 80 to 120 parts of lycium ruthenicum, 30 to 60 parts of brown sugar, 30 to 60 parts of honey and 8 to 10 parts of saccharomyces cerevisiae. The fermented oral thick paste adopts a fermentation mode, and compared with traditional medicine extraction modes such as decoction and traditional Chinese medicine extraction, the fermented oral thick paste can promote release and conversion of effective components of traditional Chinese medicines, improve the absorption and utilization rate and promote synthesis and secretion of testosterone of reproductive injury rats; the whole formula is combined with medicine and food, strains and components are synergistic, qi and blood, yin and yang are combined, multiple viscera are coordinated, and the effects of reinforcing kidney to strengthen yang, invigorating spleen to replenish qi, balancing yin and yang, reducing liver toxicity, relieving fatigue and strengthening physique are jointly achieved.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine, and particularly relates to an epimedium herb fermented paste for improving male kidney function and a preparation method thereof. Background Art

[0002] In recent years, due to the accelerated pace of modern life, people's work and life pressure has gradually increased. Coupled with the influence of long-term sitting, lack of exercise, staying up late, smoking, drinking and other bad living habits, many middle-aged men have insufficient sleep and physical overdraft, and show symptoms of low kidney function such as fatigue, weakness in the waist and knees, and sexual dysfunction.

[0003] In response to this situation, Western medicine, Chinese medicine, and a combination of Chinese and Western medicine are currently used. Although Western medicine is effective in the short term, its long-term effect is limited and can only relieve surface symptoms. If you want to maintain good results, you need to take the medicine for a long time, but it is easy to develop drug resistance. Chinese medicine currently generally uses some kidney-tonifying and aphrodisiac drugs, such as those containing epimedium and Morinda officinalis. However, in recent years, reports that epimedium and its compound preparations may cause liver damage or adverse reactions have shown an increasing trend. Many studies have confirmed that long-term or high-dose use of drugs containing epimedium may cause liver damage. Therefore, how to reduce drug toxicity while using the above-mentioned drugs to achieve the effect of strengthening the kidney and body is an urgent problem to be solved. Summary of the invention

[0004] In view of the defects and problems of current drugs for treating male renal function diseases, the present invention provides a fermented paste of whole herb of Epimedium for improving male renal function and a preparation method thereof.

[0005] The invention provides a fermented epimedium whole herb paste for improving male kidney function. The paste comprises the following raw materials in parts by weight: 900-1200 parts of epimedium whole herb, 600-800 parts of black rice, 80-120 parts of black beans, 80-120 parts of psoralea corylifolia, 40-60 parts of leek seeds, 150-200 parts of eucommia leaves, 40-60 parts of salt dodder seeds, 80-120 parts of black wolfberries, 30-60 parts of black sugar, 30-60 parts of honey and 8-10 parts of brewer's yeast.

[0006] The above-mentioned fermented paste of whole herb of epimedium for improving male kidney function comprises the following raw materials in parts by weight: 1000 parts of whole herb of epimedium, 700 parts of black rice, 100 parts of black beans, 100 parts of psoralea corylifolia, 50 parts of leek seeds, 180 parts of eucommia leaves, 50 parts of salt dodder seeds, 100 parts of black wolfberry, 30-60 parts of brown sugar, 30-60 parts of honey, and 8-10 parts of brewer's yeast.

[0007] The above-mentioned fermented epimedium whole herb paste for improving male kidney function also includes 8-10 parts of aroma yeast.

[0008] The above-mentioned Epimedium whole herb fermented paste for improving male kidney function comprises 10 parts of brewer's yeast.

[0009] The present invention also provides a method for preparing a fermented paste of whole herb Epimedium for improving male renal function, comprising the following steps:

[0010] S1. Weigh the whole herb of Epimedium, black rice, black beans, psoralea corylifolia, leek seeds, eucommia leaves, salt dodder seeds and black wolfberry according to weight ratio, remove impurities, sterilize and set aside;

[0011] S2, soak the black rice in cold water to fully absorb water and then steam it; after steaming, take it out and spread it flat, dry it to room temperature and set it aside;

[0012] S3, black beans, leek seeds and salt dodder seeds are mixed and crushed to 80-100 mesh, and then mixed with black rice dried to room temperature and placed in a fermentation tank;

[0013] S4, adding activated brewer's yeast and aroma yeast to the fermentation tank, then adding black wolfberry and 4.5-5.5 kg of warm boiled water to carry out aerobic fermentation;

[0014] S5. After 24 hours of fermentation, seal with plastic wrap or plastic film, and then let it ferment for 8 to 10 days until the supernatant in the glass fermentation tank is higher than the thickness of the solids;

[0015] S6, crushing the whole herb of Epimedium to 5-10 mesh, crushing the leaves of Eucommia ulmoides to 10-20 mesh, and frying Psoralea corylifolia in an iron pan until fragrant and crushing it to 80-100 mesh;

[0016] S7, adding the crushed whole herb of Epimedium, Eucommia leaf and Psoralea corylifolia to the fermentation tank of S5, stirring evenly, sealing and continuing to stand and ferment for 10-15 days to obtain a fermentation liquid;

[0017] S8, filtering the fermentation liquid to obtain a filtrate, concentrating the filtrate by rotary evaporation to obtain a distillate and a concentrate; centrifuging the concentrate to obtain a supernatant; and redispersing the distillate to obtain a redispersed liquid;

[0018] S9. Mix the supernatant and the redistilled liquid in proportion, add brown sugar and honey, and stir evenly to obtain the fermented paste.

[0019] In the method for preparing the fermented epimedium whole herb paste for improving male kidney function, in step S2, black rice is soaked for 6-8 hours, and the water is required to cover the rice by 1-2 cm so that the black rice can fully absorb water. Then, the water is drained and the rice is steamed for 1-1.5 hours until the black rice is cooked.

[0020] In the method for preparing the fermented paste of whole herb of Epimedium for improving male kidney function, black wolfberry and warm water are added in step S4, stirred evenly, and then sealed with sterile gauze to ensure ventilation in the early stage and fermented for 24 hours, stirring 1-2 times during the period.

[0021] The method for preparing the fermented paste of whole herb of Epimedium for improving male kidney function is as follows: the fermentation tank is placed at 30-33°C for fermentation throughout the whole process.

[0022] The above-mentioned method for preparing the fermented paste of whole herb of Epimedium for improving male kidney function, in step S8, the fermentation liquid is filtered to obtain a filtrate, the filtrate is concentrated by rotary evaporation at a ratio of 3:1 to obtain a distillate and a concentrate; the concentrate is centrifuged at 8000r / min for 5 minutes to obtain the supernatant; the distillate is redistilled at a ratio of 1:9 to obtain a redistilled liquid.

[0023] In the above-mentioned method for preparing the fermented paste of the whole herb of Epimedium for improving male kidney function, in step S9, the supernatant and the redistilled liquid are compounded in a ratio of 5:1, and then brown sugar and honey are added and stirred evenly to obtain the fermented paste.

[0024] The fermented whole herb epimedium paste of the present invention is based on the basic principles of warming and nourishing kidney yang, nourishing kidney yin, strengthening spleen and protecting liver, and regulating qi and blood. The whole herb epimedium is used as the main ingredient, eucommia leaf and black rice are used as the secondary ingredients, psoralea corylifolia, salt dodder seed and leek seeds are used as the auxiliary ingredients, and black beans and black wolfberries are used as the envoys. The medicine and food are combined, qi and blood, yin and yang are given equal importance, and multiple organs are coordinated to achieve the effects of nourishing the kidney and invigorating yang, strengthening the spleen and replenishing qi, balancing yin and yang, reducing liver toxicity, relieving fatigue, and strengthening physical fitness.

[0025] Epimedium in the prescription, also known as Xianlingpi, is warm in nature and has the effects of warming the kidney and strengthening yang, strengthening tendons and bones, and removing rheumatism. Eucommia leaves are sweet and slightly spicy, warm in nature, and enter the kidney and liver meridians. They have the effects of nourishing the liver and kidney, strengthening muscles and bones, and protecting the liver and kidney. Psoralea corylifolia is bitter and spicy, warm in nature, and enters the kidney and spleen meridians. It has the effects of tonifying the kidney and assisting yang, consolidating essence and reducing urine, and warming the spleen and stopping diarrhea. Salt dodder seeds are sweet and warm in nature, and enter the liver and kidney meridians. They have the effects of tonifying the liver and kidney, improving eyesight and stabilizing pregnancy. Leek seeds are spicy and sweet, warm in nature, and enter the liver and kidney meridians. They have the effects of tonifying the kidney and assisting yang, consolidating essence and reducing urine, warming the stomach and relieving pain, and promoting blood circulation and dispersing blood stasis. Black rice is a food and medicine with rich nutrients, especially minerals such as iron and zinc, and multiple vitamins, which can help improve physical fitness. In this recipe, it is used together with black beans, leek seeds and salt dodder as a fermentation matrix to provide fermentation conditions for brewing yeast and aroma yeast, and on the other hand, it plays the role of assisting the main and secondary drugs. Black beans are rich in protein and nutrients. In this recipe, they are used as a guiding drug to not only reconcile the various drugs, but also as a traditional Chinese medicine base material for strain fermentation with leek seeds and salt dodder, providing nutrients for strain fermentation. Black wolfberry nourishes the liver and kidneys, improves eyesight, and has antioxidant properties that can protect cells from free radical damage. While playing a health-care role, it can guide other drugs in the recipe to play a better role and reconcile the various drugs as a guiding drug.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] 1. The prescription of the present invention uses the whole herb of Epimedium as the main ingredient, with Eucommia ulmoides leaves and black rice as the subordinate ingredients, with Psoralea corylifolia, Cuscuta australis and Allium leek seeds as the auxiliary ingredients, and with black beans and black wolfberries as the messengers. The prescription combines medicine and food, pays equal attention to qi and blood, yin and yang, and coordinates multiple organs. While tonifying the kidney, it pays attention to the functions of other organs, such as the liver, spleen, stomach, etc., to ensure the balance and harmony of the whole body function. The use of the whole herb of Epimedium can give full play to the effective ingredients of Epimedium in different tissues and parts, cooperate with other drugs, exert its effects from multiple targets, and improve the therapeutic effect of rats with reproductive injury.

[0028] 2. The present invention uses black rice, black beans, leek seeds and salt dodder seeds as fermentation substrates for the strains, utilizes the different fermentation characteristics and metabolic features of the composite strains, produces a variety of active enzymes during the fermentation process, and converts the complex macromolecular substances in the medicine into small molecular substances that are easily absorbed; at the same time, the fermentation strains and the traditional Chinese medicine fermentation substrates are used as independent medicines, and the strains and the traditional Chinese medicine substrates work together to maximize the effects of tonifying the kidney and yang, strengthening the spleen and replenishing qi, and relieving fatigue.

[0029] 3. The present invention adopts biological fermentation to prepare fermented paste. Compared with traditional drug extraction methods such as boiling and Chinese medicine extraction, the fermentation method is used to give full play to the effect of the strain, which can not only make full use of the drug residues, maximize the utilization of drugs, and promote the release of effective ingredients of Chinese medicine; at the same time, the macromolecular substances in the Chinese medicine will be catalyzed and hydrolyzed by enzymes produced by microorganisms, and the effective ingredients can also be transformed by microorganisms, thereby improving the absorption and utilization rate, promoting the synthesis and secretion of testosterone in rats with reproductive damage, and improving reproductive capacity.

[0030] 4. The present invention adopts brewer's yeast and aroma yeast for co-fermentation, which can effectively increase the content of active ingredients in the formula, enhance the content of active ingredients and pharmacological activity of medicinal materials, and achieve synergistic effects between various strains, between various components, and between strains and drug components, alleviate liver toxicity, reduce the content of toxic substances while promoting the dissolution of active ingredients, improve the safety of drugs, and play a role in reducing toxicity and increasing efficacy. DETAILED DESCRIPTION

[0031] The technical solution of the present invention is further described below in conjunction with specific embodiments.

[0032] Example 1: This example provides a fermented paste of whole herb of epimedium for improving male kidney function, including 1000g of whole herb of epimedium, 700g of black rice, 100g of black beans, 100g of psoralea corylifolia, 50g of leek seeds, 180g of eucommia leaves, 50g of salt dodder seeds, 100g of black wolfberry, 50g of brown sugar, 40g of honey, 10g of brewer's yeast, and 10g of aroma yeast; wherein the brewer's yeast and the aroma yeast are both from Angel Yeast.

[0033] The preparation method is:

[0034] S1. Weigh the whole herb of Epimedium, black rice, black beans, psoralea corylifolia, leek seeds, eucommia leaves, salt dodder seeds and black wolfberry according to weight ratio, remove impurities, sterilize and set aside;

[0035] S2, soak the black rice for 6-8h to fully absorb water and then steam it; after steaming, take it out and spread it flat, dry it to room temperature and then use it;

[0036] S3, black beans, leek seeds and salt dodder seeds are mixed and crushed to 80-100 mesh, and then mixed with black rice dried to room temperature and placed in a fermentation tank;

[0037] S4, add activated brewer's yeast and aroma yeast to the fermentation tank, then add black wolfberry and 4.5-5.5 kg of warm boiled water, stir evenly and seal with sterile gauze to ensure ventilation in the early stage and ferment for 24 hours, stirring 1-2 times during the fermentation;

[0038] S5. After 24 hours of fermentation, seal with plastic wrap or plastic film, and then let it ferment for 8 to 10 days until the supernatant in the glass fermentation tank is higher than the thickness of the solids;

[0039] S6. Crush the whole herb of Epimedium to 5-10 mesh, crush the leaves of Eucommia ulmoides to 10-20 mesh, and stir-fry the Psoralea corylifolia in an iron pan until fragrant and then crush to 80-100 mesh;

[0040] S7, adding the crushed whole herb of Epimedium, Eucommia leaf and Psoralea corylifolia to the fermentation tank of S5, stirring evenly, sealing and continuing to stand and ferment for 10-15 days to obtain a fermentation liquid;

[0041] S8, the fermentation liquid is filtered to obtain a filtrate, and the filtrate is concentrated by rotary evaporation at a ratio of 3:1 to obtain a distillate and a concentrate (i.e., 1 kg of liquid is rotary evaporated to obtain 750 mL of distillate and 250 mL of concentrate); the concentrate is centrifuged at 8000 r / min for 5 min to obtain a supernatant; the distillate is redistilled at a ratio of 1:9 to obtain a redistilled liquid (i.e., 1 kg of distillate is obtained to obtain 100 mL of redistilled liquid);

[0042] S9. Mix the supernatant and the redistilled liquid in a ratio of 5:1, add brown sugar and honey, and stir evenly to obtain the fermented paste.

[0043] Example 2: This example provides a fermented paste of the whole herb of Epimedium for improving male kidney function, including 1200g of the whole herb of Epimedium, 800g of black rice, 80g of black beans, 120g of Psoralea corylifolia, 50g of leek seeds, 200g of Eucommia ulmoides leaves, 50g of Salt Cuscuta australis, 100g of black wolfberry, 60g of brown sugar, 30g of honey, 10g of brewer's yeast, and 10g of aroma yeast.

[0044] The preparation method is the same as Example 1.

[0045] Example 3: This example provides a fermented paste of the whole herb of Epimedium for improving male kidney function, including 1200g of the whole herb of Epimedium, 800g of black rice, 100g of black beans, 80g of Psoralea corylifolia, 40g of leek seeds, 150g of Eucommia ulmoides leaves, 50g of Salt Cuscuta australis, 100g of black wolfberry, 60g of brown sugar, 30g of honey, 10g of brewer's yeast, and 8g of aroma yeast.

[0046] The preparation method is the same as Example 1.

[0047] Example 4: This example provides a fermented paste of the whole herb of Epimedium for improving male kidney function, including 900g of the whole herb of Epimedium, 700g of black rice, 100g of black beans, 120g of Psoralea corylifolia, 60g of leek seeds, 180g of Eucommia ulmoides leaves, 40g of Salt Cuscuta australis, 120g of black wolfberry, 30g of brown sugar, 60g of honey, 10g of brewer's yeast, and 10g of aroma yeast.

[0048] The preparation method is the same as Example 1.

[0049] Example 5: This example provides a fermented paste of the whole herb of Epimedium for improving male kidney function, including 1000g of the whole herb of Epimedium, 700g of black rice, 100g of black beans, 100g of Psoralea corylifolia, 50g of leek seeds, 200g of Eucommia ulmoides leaves, 50g of Salt Cuscuta australis, 100g of black wolfberry, 50g of brown sugar, 50g of honey, 10g of brewer's yeast, and 10g of aroma yeast.

[0050] The preparation method is the same as Example 1.

[0051] Comparative Example 1: This comparative example adopts the Chinese medicinal raw materials of Example 1, except that it does not contain brewer's yeast and aroma yeast, and adopts a direct boiling method, specifically, putting each raw material into a boiling pot, adding 5 times the amount of water in the total mass of the raw material and boiling for 1 hour, filtering and taking the filtrate, adding 5 times the amount of water to the medicinal residue and continuing to boil for 1 hour, filtering; combining the two filtrates, and concentrating by rotary evaporation at a ratio of 3:1 to obtain a distillate and a concentrate, and centrifuging the concentrate at 8000r / min for 5min to obtain a supernatant; redistilling the distillate at a ratio of 1:9 to obtain a redistilled liquid; compounding the supernatant and the redistilled liquid at a ratio of 5:1, adding brown sugar and honey, and stirring evenly.

[0052] Comparative Example 2: This comparative example uses the Chinese medicinal raw materials of Example 1, except that only brewer's yeast is used for fermentation.

[0053] Comparative Example 3: This comparative example adopts the Chinese medicinal raw materials of Example 1, except that only aroma yeast is used for fermentation.

[0054] Comparative Example 4: This comparative example uses the Chinese medicinal raw materials of Example 1, except that all the Chinese medicinal raw materials are processed and mixed uniformly and then loaded into a fermentation tank for unified fermentation.

[0055] Comparative Example 5: This embodiment provides a fermented paste of whole herb of Epimedium for improving male renal function, including 1000g of whole herb of Epimedium, 700g of black rice, 100g of black beans, 100g of Psoralea corylifolia, 50g of leek seeds, 50g of salt dodder seeds, 100g of black wolfberry, 50g of brown sugar, 50g of honey, 10g of brewer's yeast, and 10g of aroma yeast; wherein the brewer's yeast and aroma yeast are both from Angel Yeast. The preparation method is:

[0056] S1. Weigh the whole herb of Epimedium, black rice, black beans, psoralea corylifolia, leek seeds, salt dodder seeds and black wolfberry according to weight ratio, remove impurities, sterilize and set aside;

[0057] S2, soak the black rice for 6-8h to fully absorb water and then steam it; after steaming, take it out and spread it flat, dry it to room temperature and then use it;

[0058] S3, black beans, leek seeds and salt dodder seeds are mixed and crushed to 80-100 mesh, and then mixed with black rice dried to room temperature and placed in a fermentation tank;

[0059] S4, add activated brewer's yeast and aroma yeast to the fermentation tank, then add black wolfberry and 4.5-5.5 kg of warm boiled water, stir evenly and seal with sterile gauze to ensure ventilation in the early stage and ferment for 24 hours, stirring 1-2 times during the fermentation;

[0060] S5. After 24 hours of fermentation, seal with plastic wrap or plastic film, and then let it ferment for 8 to 10 days until the supernatant in the glass fermentation tank is higher than the thickness of the solids;

[0061] S6, crushing the whole herb of Epimedium to 5-10 mesh, frying Psoralea corylifolia in an iron pan until fragrant and crushing to 80-100 mesh;

[0062] S7, adding the crushed whole herb of Epimedium and Psoralea corylifolia to the fermentation tank of S5, stirring evenly, sealing and continuing to stand and ferment for 10-15 days to obtain a fermentation liquid;

[0063] S8, filter the fermentation broth to obtain a filtrate, and concentrate the filtrate by rotary evaporation at a ratio of 3:1 to obtain a distillate and a concentrate; centrifuge the concentrate at 8000 r / min for 5 min and take the supernatant; and redistill the distillate at a ratio of 1:9 to obtain a redistilled liquid;

[0064] S9. Mix the supernatant and the redistilled liquid in a ratio of 5:1, add brown sugar and honey, and stir evenly to obtain the fermented paste.

[0065] Test Example 1: Safety and anti-fatigue performance test

[0066] 10 g of the fermented ointment prepared in Example 1 was taken and diluted at a ratio of 1:10, 1:5 and 1:2, respectively, to prepare low-dose, medium-dose and high-dose test solutions.

[0067] Twenty adult male mice of 8 weeks of age and similar weight (purchased from Henan Sikebeis Biotechnology Co., Ltd.) were selected and randomly divided into 4 groups, 5 mice in each group, divided into control group, test group 1, test group 2 and test group 3, wherein the control group was gavaged with 1 mL of distilled water, test group 1 was gavaged with 1 mL of low-dose liquid, test group 2 was gavaged with 1 mL of medium-dose liquid, and test group 3 was gavaged with 1 mL of high-dose liquid; once a day, free drinking water and diet at other times, and continuous administration for 6 weeks, and experimental animal management regulations and experimental animal ethics were strictly observed during the experiment. During the period, the experimental mice were observed for abnormalities, and the administration was terminated after 6 weeks. No obvious abnormalities occurred in the mice during gavage, indicating that the fermented paste prepared by the present invention is safe.

[0068] Starting from the 7th week, each mouse was trained to swim for 10 min every day in a cylindrical plexiglass container with a height of 80 cm, a diameter of 50 cm, a temperature of 25±1°C, and a water depth of 30 cm for one week.

[0069] At week 8, a 1.5g lead weight was placed at the base of the mouse's tail to ensure that it was firmly fixed and did not affect normal swimming. The mouse was placed in a container. If the head of the mouse was completely submerged in the water for more than 10 seconds and failed to float to the surface by itself, it was recorded as exhaustion. The mouse was fished out and dried and placed in a warm place to rest. The time from entering the water to exhaustion was recorded as the endurance swimming time. The swimming time of each group of mice was averaged, and the results are shown in Table 1 below.

[0070] Table 1 Results of forced swimming test of mice in different test groups

[0071]

[0072] As can be seen from the results in Table 1, compared with the blank control group, the fermented paste of the present invention can prolong the exhaustive swimming time of mice, and as the dose increases, the exhaustive swimming time is prolonged, indicating that the fermented paste prepared by the present invention can improve the endurance of mice and has an anti-fatigue effect. However, the increase from the medium dose to the high dose is slightly smaller than the increase from the low dose to the medium dose, indicating that as the dose continues to increase, the effect increases less, and the subsequent dose is not necessarily the higher the better. There were no obvious abnormal changes in mice during gavage, indicating that the fermented paste of the present invention is highly safe.

[0073] Test Example 2: Reproductive Function Performance Test

[0074] 1. Rat modeling of reproductive dysfunction

[0075] Fifty male SD rats aged 15 weeks and weighing 380-420g were selected and purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. (License No.: SYXK (Beijing) 2022-0052). All rats were kept in an ambient temperature of 20-24°C, humidity of 40%-50%, and light-dark alternation time of 12h. Five rats were randomly selected as blank controls and 45 rats were selected as experimental groups. The experimental group was injected with diethylstilbestrol at a fixed time every day at a dose of 5 mg per kilogram, and the left and right hind biceps femoris were alternately injected. The model was continuously injected for 14 days, and the blank control group was injected with an equal amount of normal saline. During the modeling period, the rectal temperature of each rat was measured on the 3rd, 7th, 10th, and 14th days of injection, and the behavioral performance of the rats was observed after daily administration for abnormalities.

[0076] After the last administration, the behavioral performance of the rats in the experimental group and the blank control group was combined to select rats with mental depression, slow movement, reduced activity, weight loss, yellow hair, dull hair color, and increased hair loss, i.e. 38 rats with successful modeling. During the experiment, the experimental animal management regulations and experimental animal ethics were strictly followed.

[0077] 2. Reproductive function performance test

[0078] 35 rats with successful modeling were randomly selected and randomly divided into 7 groups, 5 rats in each group, namely test group 1, test group 2, test group 3, test group 4, test group 5, test group 6, and test group 7. The weight of rats in each group was weighed and recorded, and the average weight represented the initial weight of rats in this group. Test group 1 was gavaged with 5 mL of 1:5 diluted solution of fermented paste prepared in Example 1, and tests 2-6 were gavaged with equal amounts of equal proportion diluted solution prepared in comparative examples 1-5, and test group 7 was gavaged with equal amounts of drinking water. Gavage was continued for 4 weeks. During the gavage period, the diet, drinking water and feeding environment of rats in each group were normal. The physical signs, behavioral activities and abnormalities of rats in each group were observed and recorded. After 4 weeks, the weight of rats in each group was weighed and blood was collected from the tail vein to detect serum ALT and testosterone levels. The results of general behavioral changes in rats in each group are shown in Table 2 below; the results of body weight, ALT and testosterone levels are shown in Table 3 below.

[0079] Table 2 Behavioral changes of rats in different experimental groups

[0080]

[0081]

[0082] Table 3 Body weight, ALT and testosterone levels of rats in different experimental groups

[0083]

[0084] It can be seen from Table 2 and Table 3 that compared with the control group, the fermented paste prepared by the present invention can promote the physical recovery and weight gain of reproductive disorder modeling rats, and the testosterone content increases, indicating that the prescription of the present invention can promote the synthesis and secretion of testosterone in reproductive injury rats, and can effectively treat the reproductive injury of rats. In addition, the prescription of the present invention can also effectively alleviate the liver damage of modeling rats, and play the effect of reducing toxicity and increasing efficiency; compared with test 6 groups and 7 groups, the rat alanine aminotransferase (ALT) content of the drug in the gavage test 1-5 groups is significantly lower, indicating that the addition of Eucommia ulmoides leaves can work with other drugs to alleviate liver damage and protect the liver; the differences in the drug effects of each group are mainly related to the difference between the preparation process of the fermentation bacteria and the drug, and the fermentation bacteria and preparation process of the present invention are used to facilitate the dissolution and absorption of various active ingredients of the drug in the prescription, improve the content of biologically active substances in the medicinal materials, and the beneficial bacteria contained in the drug at the same time also contribute to intestinal health and promote the performance of drug effect.

[0085] One week after blood collection, 3 rats were randomly selected from each group and housed in cages, 1 rat in each cage, at a ratio of 1:1 with healthy adult female rats for 2 weeks (approximately 3 estrous cycles). The number of matings and pregnancies of each group of rats within 2 weeks were observed and recorded. The results are shown in Table 4 below.

[0086] Table 4 The number of matings and pregnancies of rats in different experimental groups

[0087]

[0088] Note: The number of matings is the sum of the mating times of 3 rats in each group within 2 weeks.

[0089] As can be seen from Table 4, the fermented paste prepared by the present invention is beneficial to the recovery of reproductive function of rats with reproductive damage, and improves the mating frequency and pregnancy rate of rats, indicating that the formula and preparation method of the present invention can effectively promote the exertion and absorption of drug efficacy, and is beneficial to improving the reproductive function of male rats with reproductive damage.

[0090] The above is only a preferred embodiment of the present invention and does not limit the present invention. The present invention does not specifically limit the specific mode of application, and the conventional medicine, health food, and food administration method in the field can be used for administration. Any modification, equivalent substitution, and improvement made within the spirit and principle of the present invention, such as adding pharmaceutically acceptable excipients to the fermented cream of the present invention to make pills, tablets, powders, syrups, etc., should be included in the protection scope of the present invention.

Claims

1. A fermented paste of whole herb of Epimedium for improving male renal function, characterized in that: The invention comprises the following raw materials in parts by weight: 900-1200 parts of whole herb of epimedium, 600-800 parts of black rice, 80-120 parts of black beans, 80-120 parts of psoralea corylifolia, 40-60 parts of leek seeds, 150-200 parts of eucommia leaves, 40-60 parts of salt dodder seeds, 80-120 parts of black wolfberries, 30-60 parts of brown sugar, 30-60 parts of honey and 8-10 parts of brewer's yeast.

2. The fermented paste of whole herb of Epimedium for improving male renal function according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 1000 parts of whole herb of epimedium, 700 parts of black rice, 100 parts of black beans, 100 parts of psoralea corylifolia, 50 parts of leek seeds, 180 parts of eucommia leaves, 50 parts of salt dodder seeds, 100 parts of black wolfberries, 30-60 parts of brown sugar, 30-60 parts of honey and 8-10 parts of brewer's yeast.

3. The fermented paste of whole herb of Epimedium for improving male renal function according to any one of claims 1 or 2, characterized in that: Also includes 8-10 portions of aroma yeast.

4. The fermented paste of the whole herb of Epimedium for improving male renal function according to claim 1, characterized in that: 10 parts of the brewer's yeast.

5. A method for preparing a fermented paste of the whole herb of Epimedium for improving male renal function as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: S1. Weigh the whole herb of Epimedium, black rice, black beans, psoralea corylifolia, leek seeds, eucommia leaves, salt dodder seeds and black wolfberry according to weight ratio, remove impurities, sterilize and set aside; S2, soak the black rice in cold water to fully absorb water and then steam it; After steaming, take out and flatten, let it cool to room temperature and set aside; S3, black beans, leek seeds and salt dodder seeds are mixed and crushed to 80-100 mesh, and then mixed with black rice dried to room temperature and placed in a fermentation tank; S4, adding activated brewer's yeast and aroma yeast to the fermentation tank, then adding black wolfberry and 4.5-5.5 kg of warm boiled water to carry out aerobic fermentation; S5. After 24 hours of fermentation, seal with plastic wrap or plastic film, and then let it ferment for 8 to 10 days until the supernatant in the glass fermentation tank is higher than the thickness of the solids; S6, crushing the whole herb of Epimedium to 5-10 mesh, crushing the leaves of Eucommia ulmoides to 10-20 mesh, and frying Psoralea corylifolia in an iron pan until fragrant and crushing it to 80-100 mesh; S7, adding the crushed whole herb of Epimedium, Eucommia leaf and Psoralea corylifolia to the fermentation tank of S5, stirring evenly, sealing and continuing to stand and ferment for 10-15 days to obtain a fermentation liquid; S8, filtering the fermentation liquid to obtain a filtrate, concentrating the filtrate by rotary evaporation to obtain a distillate and a concentrate; centrifuging the concentrate to obtain a supernatant; and redispersing the distillate to obtain a redispersed liquid; S9. Mix the supernatant and the redistilled liquid in proportion, add brown sugar and honey, and stir evenly to obtain the fermented paste.

6. The method for preparing the fermented paste of whole herb of Epimedium for improving male renal function according to claim 5, characterized in that: In step S2, the black rice is soaked for 6-8 hours, and the water is required to cover the rice by 1-2 cm, so that the black rice can fully absorb water. Then, the water is drained and the black rice is steamed for 1-1.5 hours until the black rice is cooked.

7. The method for preparing the fermented extract of the whole herb of Epimedium for improving male renal function according to claim 5, characterized in that: In step S4, add black wolfberry and warm water, stir evenly, and seal with sterile gauze to ensure ventilation in the early stage and ferment for 24 hours, stirring 1-2 times during the period.

8. The method for preparing the fermented paste of whole herb of Epimedium for improving male renal function according to claim 5, characterized in that: The fermentation tank was placed at 30-33°C for fermentation.

9. The method for preparing the fermented extract of the whole herb of Epimedium for improving male renal function according to claim 5, characterized in that: In step S8, the fermentation liquid is filtered to obtain a filtrate, and the filtrate is concentrated by rotary evaporation at a ratio of 3:1 to obtain a distillate and a concentrate; the concentrate is centrifuged at 8000 r / min for 5 minutes to obtain a supernatant; the distillate is redistilled at a ratio of 1:9 to obtain a redistilled liquid.

10. The method for preparing the fermented paste of whole herb of Epimedium for improving male renal function according to claim 9, characterized in that: In step S9, the supernatant and the redistilled liquid are mixed in a ratio of 5:1, and then brown sugar and honey are added and stirred evenly to obtain a fermented paste.

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