Bacterial colony screening operation platform and screening process

Abstract

The present invention relates to the technical field of microbial colony screening, and discloses a colony screening operation platform and a screening process, the colony screening operation platform comprises: a movable table performing lifting movement and translation movement; the lifting columns vertically penetrate through the movable table and vertically move; the rotary drum is horizontally and rotatably connected to the lower end of the lifting column and horizontally rotates; the screening needle is vertically and rotatably connected to the lower end of the rotary drum and vertically swings; a plurality of bacterial colonies can be selected at the same time each time, and the bacterial colony screening speed and efficiency are remarkably improved; the screening needle can rotate and swing to correspond to bacterial colonies at all positions in a circular range, so that the accuracy of bacterial colony screening is ensured; the lower ends of the swinging screening needles are located at the same height through the lifting column, and synchronous and efficient bacterial colony selection is guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of microbial colony screening, and in particular to a colony screening operation platform and a screening process. Background Art

[0002] Different types of bacteria will form colonies with different characteristics under specific culture conditions, such as the size, shape, color, edge, surface condition, and ridge of the colonies. Screening colonies with specific characteristics will help to further study the various characteristics and functions of bacteria. For example, screening colonies that can grow under specific environmental conditions can study the adaptability mechanism of the bacteria to this environment; screening colonies with special metabolic capabilities, such as colonies that can decompose specific substances, can explore their metabolic pathways and the characteristics of related enzymes; selecting colonies from the edge of the inhibition zone can often obtain bacteria with certain drug resistance, and then study their drug resistance mechanism, providing a basis for clinical treatment and prevention of bacterial infections.

[0003] In microbiological research and applications, it is often necessary to obtain pure cultures, that is, cultures containing only one type of microorganism. By screening a single colony, it can be inoculated into a new culture medium for culture, thereby obtaining a pure culture represented by the colony, avoiding interference from other bacteria, and facilitating in-depth research and accurate analysis of the microorganism. In the fields of scientific research, medicine, and environmental protection, strains with specific functions need to be screened out. For example, in the development of antibiotics, strains that can produce specific antibiotics must be selected from a large number of mixed bacterial communities; in environmental pollution control, strains that can degrade specific pollutants must be selected; in drug development and production, strains that can produce specific drugs must be selected to meet different research and application needs.

[0004] In traditional colony screening, researchers manually select colonies from culture dishes and place them in new culture media on well plates. Manual screening is time-consuming and labor-intensive, with low efficiency and accuracy, and high scientific research and production costs. The automatic colony selection in the prior art uses visual technology to complete the acquisition and positioning of target colony images, and automatically selects them into well plates through automatic moving devices and screening needles. In order to avoid cross-infection, the prior art uses metal screening needles for repeated screening, which requires repeated disinfection of the screening needles, such as the patented automatic colony selection device with publication number CN218059015U; this increases the time for colony screening and limits the screening efficiency. There are also disposable plastic needles that can effectively avoid cross-infection, such as the patented automatic colony selection instrument with publication number CN108641903B, which uses disposable needles, omits the disinfection step, and thereby improves screening efficiency. However, in the prior art, whether it is a sterilization-type automatic colony screening structure or a colony screening structure with a replacement needle, only one colony can be selected each time the colony screening is performed, and the colony screening is achieved through a large number of repeated actions. Although some prior arts have improved the colony screening structure, action process and steps, and shortened the selection time interval, only one colony is selected each time, and the efficiency improvement is limited, which limits the further improvement of the screening efficiency. Summary of the invention

[0005] The purpose of the present invention is to provide a colony screening operation platform and a screening process in order to solve the above-mentioned problems in the prior art.

[0006] To achieve the above object, the present invention provides the following technical solutions: A colony screening operation platform, comprising: A movable table for lifting and translational movement; A plurality of lifting columns vertically passing through the movable platform and moving vertically; A rotating drum, horizontally connected to the lower end of the lifting column and horizontally rotating; The screening needle is vertically rotatably connected to the lower end of the rotating drum and vertically swings.

[0007] Furthermore, it also includes: A disposable needle, wherein the screening needle is inserted into the inner side of the upper end of the disposable needle; A needle plate for placing disposable needles, wherein the horizontal spacing of the disposable needles placed on the needle plate is the same as the spacing of the lifting columns on the movable table; A waste box for collecting used disposable needles, wherein the outer ring side of the upper end of the disposable needles is provided with an annular protrusion; a plurality of racket-shaped needle unloading holes are provided on the upper wall of the waste box; the needle unloading holes are the same in number and spacing as the lifting columns.

[0008] Furthermore, the screening needle is a hollow tubular structure; a hose is connected to the upper end of the screening needle, the hose passes upward through the rotating drum and the lifting column, there is a gap between the hose and the inner side of the rotating drum, the hose is fixedly connected to the lifting column, and an airbag is connected to the upper end of the hose.

[0009] Furthermore, a support plate is arranged in parallel and at intervals on the upper side of the movable platform, the airbag is located on the upper side of the support plate, and the upper end of the hose passes through the support plate and is connected to the airbag; a liftable pressure frame is provided on the upper side of the support plate to apply pressure to the airbag, and the pressure frame is located on the upper side of the airbag.

[0010] Furthermore, a lead screw and a fourth servo motor driving the lead screw to rotate are vertically installed on the upper side of the center of the support plate; the lead screw passes through the pressure frame and is threadedly connected to the center of the pressure frame, and a pressure plate corresponding to the airbag is provided on the lower side of the edge of the pressure frame.

[0011] Furthermore, it also includes a table; a door-shaped left and right linear module is provided above the table; a front and rear linear module is provided on the upper side of the table for moving the left and right linear modules back and forth; it also includes a square cylinder, a movable table is vertically raised and lowered inside the square cylinder, and an automatic telescopic rod is connected between the movable table and the upper wall of the square cylinder; the left and right linear modules enable the square cylinder to move left and right above the table; a culture dish, a well plate containing new culture medium and a visual camera corresponding to the culture dish are placed on the upper side of the table.

[0012] Furthermore, the movable platform is a hollow structure, and a vertical rack is provided on one side of the lifting column; a first gear meshing with the rack and a first servo motor driving the first gear to rotate are provided in the movable platform.

[0013] Furthermore, a gear ring is provided on the outer ring side of the upper end of the rotating drum, and a second gear meshing with the gear ring and a second servo motor driving the second gear to rotate are provided at the lower end of the lifting column.

[0014] Furthermore, a U-shaped frame is provided at the upper end of the screening needle, the U-shaped frame is rotatably connected to the lower end of the rotating drum, a third gear is connected to one side of the U-shaped frame, and a fourth gear meshing with the third gear and a third servo motor driving the fourth gear to rotate are provided at the lower end of the rotating drum.

[0015] The present invention provides the following technical solution: a screening process of a colony screening operation platform, comprising the following steps: S1, the movable table moves to the area above the colony to be selected; S2, rotating the drum horizontally and swinging the screening needle vertically until the lower end of the screening needle vertically corresponds to the bacterial colony to be selected; S3, after the lower end of each screening needle corresponds to the bacterial colony, the lifting column connected above the screening needle is raised and lowered so that the lower end of each screening needle is at the same height; S4, the movable platform descends so that the lower end of the screening needle reaches the bacterial colony, and multiple bacterial colonies are selected at the same time; S5. The movable table carries multiple colonies and moves to the upper side of the new culture medium. During the movement, the lifting column is reset, the drum rotates, and the screening needle swings until the lower end of the screening needle corresponds to the upper side of each independent new culture medium; then the colonies are released.

[0016] Compared with the prior art, the present invention has the following beneficial effects: The present invention can select multiple colonies at the same time each time, significantly improving the speed and efficiency of colony screening; the screening needle of the present invention can rotate and swing, thereby corresponding to the colonies at various positions within the circular range, ensuring the accuracy of colony screening; the lower ends of the screening needles after swinging are located at the same height through the lifting column, ensuring synchronous and efficient selection of colonies. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0018] Figure 2 It is a front view schematic diagram of the present invention.

[0019] Figure 3 It is a top view schematic diagram of the present invention.

[0020] Figure 4 It is a schematic diagram of the square tube connection structure of the present invention.

[0021] Figure 5 It is a schematic diagram of the cross-section structure of the square tube of the present invention.

[0022] Figure 6 It is a three-dimensional schematic diagram of the screening structure of the present invention.

[0023] Figure 7 It is a front view schematic diagram of the screening structure of the present invention.

[0024] Figure 8 It is a schematic diagram of the three-dimensional structure of the movable platform and the support plate of the present invention from the first perspective.

[0025] Fig. 9 It is a schematic diagram of the three-dimensional structure of the movable platform and the support plate of the present invention from a second viewing angle.

[0026] Fig.10 It is a schematic diagram of the first viewing angle of the connection structure of the lifting column, the rotating drum and the screening needle of the present invention.

[0027] Fig.11 It is a schematic diagram of the connection structure of the lifting column, the rotating drum and the screening needle from a second viewing angle of the present invention.

[0028] Fig.12 It is a schematic diagram of the connection structure of the lifting column, the rotating drum and the screening needle from the third viewing angle of the present invention.

[0029] Fig.13 for Fig.12Enlarged schematic diagram in the middle.

[0030] Fig.14 It is a schematic diagram of a disposable needle of the present invention.

[0031] In the figure: 1, movable table; 2, lifting column; 3, rotating drum; 4, screening needle; 5, disposable needle; 6, hose; 7, support plate; 8, air bag; 9, pressing frame; 10, column hole; 11, rack; 12, first gear; 13, first servo motor; 14, limit plate; 15, gear ring; 16, second gear; 17, second servo motor; 18, U-shaped frame; 19, third gear; 20, fourth gear; 21, third servo motor machine; 22. notch; 23. support rod; 24. pressure plate; 25. lead screw; 26. fourth servo motor; 27. outer ring frame; 28. square cylinder; 29. ​​automatic telescopic rod; 30. movable seat; 31. balance block; 32. annular protrusion; 33. table; 34. left and right linear modules; 35. front and rear linear modules; 36. culture dish; 37. visual camera; 38. orifice plate; 39. waste box; 40. needle plate; 41. unloading needle hole. DETAILED DESCRIPTION

[0032] In order to make the purpose, technical scheme and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention, that is, the embodiments described are only part of the embodiments of the present invention, rather than all of the embodiments. The components of the embodiments of the present invention described and shown in the drawings herein can be arranged and designed in various different configurations.

[0033] It should be noted that relational terms such as "first", "second", "third" and "fourth" are used only to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprises" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0034] In the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected" and "connected" that may appear should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, or it can be the internal communication of two components. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0035] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the term "provided with" that may appear should be understood in a broad sense. For example, the object "provided with" may be a part of the body, or may be arranged separately from the body and connected to the body, and the connection may be a detachable connection or an inseparable connection. For those skilled in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0036] Specific embodiments of the colony screening operation platform and screening process provided by the present invention: See also Figure 1-14 The colony screening operation platform includes a table 33, a movable table 1, a plurality of lifting columns 2, a rotating drum 3, a screening needle 4, a square cylinder 28, a disposable needle 5, a needle plate 40 and a waste box 39.

[0037] A gate-shaped left and right linear module 34 is provided above the table 33; a front and rear linear module 35 is provided on the upper side of the table 33 to enable the left and right linear module 34 to move forward and backward; the linear module structure driven by the linear force is a prior art and will not be described in detail in this embodiment. A culture dish 36, a well plate 38 for accommodating new culture medium, and a visual camera 37 corresponding to the culture dish 36 are placed on the upper side of the table 33. Specifically, a vertical plate is provided on the upper side of the rear edge of the table 33, a horizontal plate is provided on the front side of the upper end of the vertical plate, and a visual camera 37 is installed at the front end of the horizontal plate; a support platform is provided on the upper side of the table 33, a light board is provided on the side of the upper side of the support platform, the culture dish 36 is placed on the upper side of the light board, and the visual camera 37 is located on the upper side of the culture dish 36.

[0038] The left and right linear modules 34 enable the upper part of the gate-shaped left and right linear modules 34 of the square tube 28 to move horizontally left and right above the table 33; the rear side of the square tube 28 is connected to a movable seat 30 that moves left and right along the left and right linear modules 34, and the rear side of the movable seat 30 is connected to a balancing block 31. The balancing block 31 balances the force on the front and rear sides of the movable seat 30, thereby making the left and right movement of the movable seat 30 and the square tube 28 more stable and smooth.

[0039] The movable table 1 moves up and down and moves in translation; the movable table 1 moves in translation following the square cylinder 28 driven by the left and right linear modules 34 and the front and rear linear modules 35, and can correspond to the culture dish 36, the well plate 38, etc., so as to accurately select and release the colonies. The movable platform 1 is lifted and lowered vertically inside the square tube 28. The movable platform 1 is a circular disc structure. The outer ring side of the movable platform 1 is connected to an outer ring frame 27 in the shape of an annular plate with an outer square and an inner circle. Vertical guide rods are provided at the four corners of the inner side of the square tube 28. The guide rods pass through the outer ring frame 27. Light holes are provided at the four corners of the outer ring frame 27. The outer ring frame 27 is lifted and lowered along the guide rods through the light holes.

[0040] An automatic telescopic rod 29 is connected between the movable platform 1 and the upper wall of the square tube 28; four automatic telescopic rods 29 are provided and move synchronously. The automatic telescopic rod 29 is an electric telescopic rod. Specifically, the telescopic end of the automatic telescopic rod 29 is directly connected to the upper middle part of the edge of the outer ring frame 27; the telescopic end of the automatic telescopic rod 29 drives the outer ring frame 27 and the movable platform 1 to rise and fall. After the movable platform 1 rises, there is a vertical gap with the equipment on the upper side of the table 33 and it can move freely in parallel. After it descends, it can contact or cooperate with various equipment on the upper side of the table 33, such as the culture dish 36 and the well plate 38.

[0041] Several lifting columns 2 vertically pass through the movable platform 1 and move vertically; the cross section of the lifting columns 2 is rectangular, and the movable platform 1 is provided with several column holes 10 corresponding to the lifting columns 2 for the lifting columns 2 to pass through, and the cross section of the column holes 10 is rectangular, so that the lifting columns 2 can only be lifted and lowered but not rotated horizontally. The lifting columns 2 are evenly spaced on the movable platform 1. In this embodiment, the number of the lifting columns 2 is three, and they are evenly distributed around the circumference of the movable platform 1.

[0042] The movable platform 1 is a hollow structure, and the upper and lower walls of the movable platform 1 are provided with column holes 10. A vertical groove is provided on one side of the lifting column 2, and a vertical rack 11 is provided in the groove; a first gear 12 meshing with the rack 11 and a first servo motor 13 driving the first gear 12 to rotate are provided in the movable platform 1; three groups of the first gear 12 and the first servo motor 13 are provided to drive three lifting columns 2 to rise and fall respectively.

[0043] Limiting plates 14 are provided at the upper and lower ends of the lifting column 2. The cross-sectional area of ​​the limiting plates 14 is larger than the cross-sectional area of ​​the lifting column 2 and larger than the cross-sectional area of ​​the column hole 10, so as to limit the lifting and lowering of the lifting column 2 and prevent the lifting column 2 from detaching from the movable platform 1.

[0044] The rotating drum 3 is horizontally connected to the lower end of the lifting column 2 and rotates horizontally; a rotating support supporting the horizontal rotation of the rotating drum 3 is provided at the lower side of the lifting column 2, and the inner side of the upper end of the rotating drum 3 is horizontally connected to the rotating support, and the rotating support can support the rotating drum 3 to rotate around its own vertical center. A gear ring 15 is provided on the outer ring side of the upper end of the rotating drum 3, and a second gear 16 meshing with the gear ring 15 and a second servo motor 17 driving the second gear 16 to rotate are provided at the lower end of the lifting column 2.

[0045] The second servo motor 17 is higher than the lower side of the lifting column 2, and the second servo motor 17 is located on a horizontal side of the lower end of the lifting column 2; a notch 22 is provided on one side of the column hole 10 on the lower wall of the movable platform 1 to cooperate with the second servo motor 17 and allow the second servo motor 17 to pass through. The second servo motor 17 can pass through the notch 22, thereby reducing the vertical space occupied and making the structure more compact.

[0046] The screening needle 4 is vertically rotatably connected to the lower end of the drum 3 and vertically swings. A U-shaped frame 18 is provided at the upper end of the screening needle 4. Specifically, the upper end of the screening needle 4 passes through the middle of the U-shaped frame 18, and both ends of the U-shaped frame 18 are rotatably connected to both sides of the lower end of the drum 3 coaxially. A third gear 19 is connected to one side of the U-shaped frame 18, and a fourth gear 20 meshing with the third gear 19 and a third servo motor 21 driving the fourth gear 20 to rotate are provided at the lower end of the drum 3.

[0047] The third servo motor 21 drives the fourth gear 20 to rotate, thereby driving the third gear 19, the U-shaped frame 18 and the screening needle 4 to rotate, so that the screening needle 4, which was originally in a vertical state, can swing vertically and tilt at a certain angle, so as to correspond to more colonies in the culture dish 36; the second servo motor 17 drives the second gear 16 to rotate, thereby causing the gear ring 15 and the rotating drum 3 to rotate on the lower side of the lifting column 2, so that the inclined screening needle 4 can rotate horizontally, so that the lower end of the screening needle 4 can correspond to all colonies within a circular range in the culture dish 36.

[0048] The screening needles 4 on the lower side of each lifting column 2 can correspond to all colonies within a circular range in the culture dish 36. When the screening needles 4 in the vertical state cannot correspond exactly to the colonies in the culture dish 36, the screening needles 4 can automatically correspond to the colonies to be selected through the swinging, tilting and horizontal rotation, thereby meeting the needs of selecting multiple colonies at the same time.

[0049] Because one screening needle 4 needs to correspond to one colony in the culture dish 36 at the same time; it is possible that the screening needle 4 can correspond to the colony without swinging, or it is possible that the screening needle 4 needs to swing at different angles to correspond to the colony. Therefore, when all the screening needles 4 on the lower side of the movable platform 1 correspond vertically to the colonies, the lower ends of the screening needles 4 are generally at different heights. At this time, if the movable platform 1 drives the screening needles 4 to descend, the lower ends of the screening needles 4 cannot reach the liquid surface of the culture dish 36 at the same time, which is not conducive to multiple screening needles 4 selecting colonies at the same time.

[0050] Therefore, after all the screening needles 4 are vertically aligned above the colonies, before the movable platform 1 descends, the height of the lower ends of the screening needles 4 is adjusted by lifting and lowering the lifting columns 2. By lifting and lowering the lifting columns 2, the heights of the lower ends of all the screening needles 4 can be made the same or similar. Then the movable platform 1 drives all the screening needles 4 to descend, so that the lower ends of all three screening needles 4 reach the colonies on the liquid surface of the culture dish 36 at the same time, which is conducive to accurately and simultaneously obtaining the colonies.

[0051] The screening process of the colony screening operation platform includes the following steps: the movable platform 1 moves to the colony area that needs to be selected, that is, above the culture dish 36; the rotating drum 3 rotates horizontally and the screening needle 4 swings vertically until the lower end of the screening needle 4 corresponds vertically to the colony that needs to be selected; after the lower end of each screening needle 4 corresponds to the colony, the lifting column 2 connected above the screening needle 4 is raised and lowered so that the lower end of each screening needle 4 is at the same height; the movable platform 1 descends so that the lower end of the screening needle 4 reaches the colony, and multiple colonies are selected at the same time; the movable platform 1 carries multiple colonies and moves to the new culture medium, that is, the upper side of the well plate 38. During the movement, the lifting column 2 is reset, the rotating drum 3 rotates, and the screening needle 4 swings until the lower end of the screening needle 4 corresponds to the upper side of each independent new culture medium; then the colony is released.

[0052] In the prior art, the image of the colony of the culture dish 36 is obtained by the visual camera 37, and then the selectable colony position in the image is identified by the computer, and then the action structure moves to the colony position to select the colony, and only one colony can be screened out each time, which is inefficient. The colony screening operation platform of this embodiment can select multiple colonies each time. Although the amount of calculation of the computer is increased, the screening time is shortened, the amount of colonies selected at a single time is increased, and the amount of colonies selected at a single time is doubled, which greatly improves the screening efficiency. Due to the disturbance effect of multiple screening needles 4 on the surface of the culture dish 36 when they are simultaneously moved, the visual camera 37 needs to collect the colony image of the culture dish 36 once after each colony is selected; when the number of colonies in the culture dish 36 is insufficient, the culture dish 36 is replaced.

[0053] In order to avoid the problem of cross infection between multiple selected colonies, in this embodiment, a disinfection method is not adopted, but a tubular disposable needle 5 is used, and the lower end of the screening needle 4 is inserted into the inner side of the upper end of the disposable needle 5; and cross infection is avoided by replacing the disposable needle 5.

[0054] New disposable needles 5 are placed on the needle plate 40, and the horizontal spacing between the disposable needles 5 placed on the needle plate 40 is the same as the horizontal spacing between the lifting columns 2 on the movable platform 1; since in this embodiment, the number of lifting columns 2 and screening needles 4 is only three, the number is small and the spacing is large, the spacing between the disposable needles 5 placed on the needle plate 40 is large; in some other embodiments, when the lifting columns 2 and screening needles 4 are arranged more and more densely, a needle plate 40 with a smaller spacing between the disposable needles 5 can be set.

[0055] The lower end of the screening needle 4 is set to a cone, and the inner side of the disposable needle 5 is set to the cone matching the screening needle 4. When the movable platform 1 descends, the screening needle 4 without the disposable needle 5 is carried down in a vertical state, and the lower end of the screening needle 4 extends into the disposable needle 5, and pressure is applied to achieve interference fit, so that the disposable needle 5 made of plastic is fixed on the outer side of the lower end of the screening needle 4. Then the movable platform 1 and the screening needle 4 rise to take away the new disposable needle 5.

[0056] In order to obtain the colonies more stably, the colonies are stably allowed to leave the culture dish 36 along with the screening needle 4. The screening needle 4 is a hollow tubular structure; the upper end of the screening needle 4 is connected with a hose 6, which passes upward through the rotating drum 3 and the lifting column 2, with a gap between the hose 6 and the inner side of the rotating drum 3, the hose 6 is fixedly connected to the lifting column 2, and the upper end of the hose 6 is connected with an air bag 8.

[0057] A support plate 7 is arranged parallel to and spaced apart from the upper side of the movable platform 1. A plurality of support rods 23 are connected between the edge of the support plate 7 and the edge of the movable platform 1. The support rods 23 are evenly distributed around the circumference. The airbag 8 is located on the upper side of the support plate 7. The upper end of the hose 6 passes through the support plate 7 and is connected to the airbag 8. The length of the hose 6 between the movable platform 1 and the support plate 7 is not less than the distance from the movable platform 1 to the support plate 7. The hose 6 realizes the function of guiding gas and transmitting gas pressure. When the lifting column 2 rises, the hose 6 can bend without affecting the transmission of the internal gas. The adaptive deformation of the hose 6 also has the advantage that the lower end of the hose 6 does not affect the swing of the screening needle 4 and is connected to the screening needle 4.

[0058] A three-spoke pressure frame 9 that can be raised and lowered to apply pressure to the airbag 8 is provided on the upper side of the support plate 7. The outer end of the pressure frame 9 is located on the upper side of the airbag 8. A pressure plate 24 corresponding to the upper side of the airbag 8 is provided on the lower side of the outer edge of the pressure frame 9. A concave surface is provided on the lower side of the pressure plate 24, which can apply pressure to the airbag 8 like a palm. A lead screw 25 and a fourth servo motor 26 that drives the lead screw 25 to rotate are vertically installed on the upper side of the center of the support plate 7; the lead screw 25 passes through the pressure frame 9 and is threadedly connected to the center of the pressure frame 9. A vertical threaded through hole threadedly connected to the lead screw 25 is provided in the center of the pressure frame 9. The fourth servo motor 26 drives the lead screw 25 to rotate to raise and lower the pressure frame 9, and the pressure plate 24 directly applies pressure and releases the airbag 8.

[0059] The disposable needle 5 replaces the lower end of the screening needle 4 to contact the bacterial colony, avoiding cross infection and eliminating the need for frequent disinfection.

[0060] Before selecting the colony, the pressing frame 9 descends and the pressing plate 24 presses out the air in the airbag 8 to flatten the airbag 8, causing the airbag 8 to deform elastically, and the air in the airbag 8 is discharged through the hose 6, the screening needle 4 and the disposable needle 5. When the movable platform 1 drives the screening needle 4 and the disposable needle 5 to descend, the lower ports of all the disposable needles 5 descend to the colony at the liquid level of the culture dish 36, causing the pressing frame 9 and the pressing plate 24 to rise, and the airbag 8 is reset under its own elasticity, generating negative pressure in the hose 6, the screening needle 4 and the disposable needle 5, sucking the colony and part of the original culture medium in the culture dish 36 into the lower end of the disposable needle 5. When the movable platform 1 carries the disposable needle 5 to the upper side of the orifice plate 38, the pressing frame 9 descends to compress and exhaust the air in the airbag 8, so that the colony in the disposable needle 5 is discharged into the independent new culture medium in the orifice plate 38. Since the spacing between the independent new culture media in each well on the well plate 38 is small, the swingable and rotatable screening needle 4 in this embodiment can adjust the swing angle and horizontal rotation angle of the screening needle 4, so that the spacing between the lower ends of each disposable needle 5 matches the spacing between the holes of the well plate 38, and then the lower end of the disposable needle 5 extends into the inner side of the upper end of the adjacent hole of the well plate 38.

[0061] The waste box 39 collects the used disposable needles 5, and an annular protrusion 32 is provided on the outer ring side of the upper end of the disposable needle 5; a plurality of racket-shaped needle unloading holes 41 are provided on the upper wall of the waste box 39; the needle unloading holes 41 are the same in number and spacing as the lifting columns 2.

[0062] After being emptied, the disposable needle 5 moves to the upper side of the waste bin 39. The screening needle 4 and the disposable needle 5 are reset to a vertical state. First, the movable platform 1 drives the screening needle 4 and the disposable needle 5 to descend, pass through the larger part of the needle unloading hole 41, until the disposable needle 5 is below the upper wall of the waste bin 39, and then translates to the smaller part of the needle unloading hole 41, the width of which is larger than the outer diameter of the screening needle 4 and smaller than the outer diameter of the annular protrusion 32 at the upper end of the disposable needle 5. Therefore, at this time, the movable platform 1 drives the screening needle 4 to move upward, and the used disposable needle 5 will fall off from the outer side of the lower end of the screening needle 4 into the waste bin 39 under the obstruction of the upper wall of the waste bin 39. Then the screening needle 4 takes a new disposable needle 5 on the upper side of the needle plate 40 for the next colony selection.

[0063] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions recorded in the aforementioned embodiments without creative work, or replace some of the technical features therein with equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims

1. A colony screening operation platform, characterized in that: include: A movable table (1) is capable of lifting and lowering movement and translational movement; A plurality of lifting columns (2) vertically passing through the movable platform (1) and moving vertically; A rotating drum (3) is horizontally rotatably connected to the lower end of the lifting column (2) and rotates horizontally; The screening needle (4) is vertically rotatably connected to the lower end of the rotating drum (3) and swings vertically.

2. The colony screening operation platform according to claim 1, characterized in that: Also includes: A disposable needle (5), wherein the screening needle (4) is plugged into the inner side of the upper end of the disposable needle (5); A needle plate (40) for placing disposable needles (5), wherein the horizontal spacing between the disposable needles (5) placed on the needle plate (40) is the same as the spacing between the lifting columns (2) on the movable platform (1); A waste box (39) for collecting used disposable needles (5), wherein an annular protrusion (32) is provided on the outer ring side of the upper end of the disposable needle (5); a plurality of racket-shaped needle unloading holes (41) are provided on the upper wall of the waste box (39); the needle unloading holes (41) are the same in number and spacing as the lifting columns (2).

3. The colony screening operation platform according to claim 1, characterized in that: The screening needle (4) is a hollow tubular structure; the upper end of the screening needle (4) is connected to a hose (6), the hose (6) passes through the rotating drum (3) and the lifting column (2) upwards, there is a gap between the hose (6) and the inner side of the rotating drum (3), the hose (6) is fixedly connected to the lifting column (2), and the upper end of the hose (6) is connected to an air bag (8).

4. The colony screening operation platform according to claim 3, characterized in that: A support plate (7) is arranged parallel to and spaced apart from the upper side of the movable platform (1); the airbag (8) is located on the upper side of the support plate (7); the upper end of the hose (6) passes through the support plate (7) and is connected to the airbag (8); a liftable pressure frame (9) for applying pressure to the airbag (8) is arranged on the upper side of the support plate (7); the pressure frame (9) is located on the upper side of the airbag (8).

5. The colony screening operation platform according to claim 4, characterized in that: A lead screw (25) and a fourth servo motor (26) for driving the lead screw (25) to rotate are vertically mounted on the upper center side of the support plate (7); the lead screw (25) passes through the pressure frame (9) and is threadedly connected to the center of the pressure frame (9); a pressure plate (24) corresponding to the airbag (8) is provided on the lower side of the edge of the pressure frame (9).

6. The colony screening operation platform according to claim 1, characterized in that: It also includes a table (33); a door-shaped left and right linear module (34) is provided above the table (33); a front and rear linear module (35) is provided on the upper side of the table (33) to enable the left and right linear module (34) to move forward and backward; it also includes a square tube (28), the movable table (1) is vertically raised and lowered inside the square tube (28), and an automatic telescopic rod (29) is connected between the movable table (1) and the upper wall of the square tube (28); the left and right linear module (34) enables the square tube (28) to move left and right above the table (33); a culture dish (36), a well plate (38) containing new culture medium, and a visual camera (37) corresponding to the culture dish (36) are placed on the upper side of the table (33).

7. The colony screening operation platform according to claim 1, characterized in that: The movable platform (1) is a hollow structure, and a vertical rack (11) is provided on one side of the lifting column (2); a first gear (12) meshing with the rack (11) and a first servo motor (13) driving the first gear (12) to rotate are provided in the movable platform (1).

8. The colony screening operation platform according to claim 1, characterized in that: A gear ring (15) is provided on the outer ring side of the upper end of the rotating drum (3), and a second gear (16) meshing with the gear ring (15) and a second servo motor (17) driving the second gear (16) to rotate are provided on the lower end of the lifting column (2).

9. The colony screening operation platform according to claim 1, characterized in that: A U-shaped frame (18) is provided at the upper end of the screening needle (4), the U-shaped frame (18) is rotatably connected to the lower end of the rotating drum (3), one side of the U-shaped frame (18) is connected to a third gear (19), and the lower end of the rotating drum (3) is provided with a fourth gear (20) meshing with the third gear (19) and a third servo motor (21) driving the fourth gear (20) to rotate.

10. The screening process of the colony screening operation platform according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1, the movable table (1) moves to the area above the colony to be selected; S2, rotating the drum (3) horizontally and swinging the screening needle (4) vertically until the lower end of the screening needle (4) vertically corresponds to the bacterial colony to be selected; S3, after the lower end of each screening needle (4) corresponds to the bacterial colony, the lifting column (2) connected above the screening needle (4) is raised and lowered so that the lower end of each screening needle (4) is located at the same height; S4, the movable platform (1) is lowered so that the lower end of the screening needle (4) reaches the bacterial colony, and multiple bacterial colonies are selected at the same time; S5. The movable table (1) carries the multiple bacterial colonies and moves to the upper side of the new culture medium. During the movement, the lifting column (2) is reset, the rotating drum (3) rotates, and the screening needle (4) swings until the lower end of the screening needle (4) corresponds to the upper side of each independent new culture medium; then the bacterial colonies are released.

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