Stem cell culture dish

Abstract

The invention relates to the technical field of cell culture, in particular to a stem cell culture dish which comprises a mounting base, a culture dish box is fixedly connected to the upper end of the mounting base, a culture dish cover is clamped to the upper end of the culture dish box, a small amount of stem cells are filled between the culture dish cover and the inner wall of the culture dish box, and an electric telescopic arm is fixedly connected to the upper end of the mounting base. The output end of the electric telescopic arm is fixedly connected with a mounting cylinder, the mounting cylinder is located over the culture dish cover, the mounting cylinder and the culture dish cover are fixedly connected through two limiting telescopic arms, the upper end of the mounting cylinder is provided with a liquid storage box, the liquid storage box is filled with a nutrient solution, and an extraction mechanism is fixedly installed between the liquid storage box and the culture dish cover. The extraction mechanism can extract a nutrient solution from the interior of the liquid storage box and convey the nutrient solution into the culture dish cover and the culture dish box, an oxygen supply mechanism is further fixedly connected between the liquid storage box and the culture dish cover, and the oxygen supply mechanism can provide oxygen for stem cells in the culture dish cover and the culture dish box.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a stem cell culture dish. Background Art

[0002] In the field of stem cell culture, traditional culture methods face many challenges. Stem cells have unique biological characteristics, and their growth, proliferation and differentiation processes have extremely stringent requirements on the microenvironment. On the one hand, stem cells need an adequate and appropriate supply of nutrient solution, which contains a variety of amino acids, vitamins, glucose and other nutrients. These substances are key raw materials for stem cells to synthesize biomacromolecules, maintain metabolic activities and perform energy conversion. For example, amino acids are used to build proteins, and proteins play an indispensable role in cell structure, signal transduction and enzymatic reactions. Glucose is the main energy source for cells, providing power for various life activities of stem cells through intracellular glycolysis and aerobic respiration.

[0003] On the other hand, oxygen supply is also crucial for stem cell culture. Stem cells use oxygen to oxidize nutrients during aerobic respiration to produce a large amount of adenosine triphosphate (ATP), providing sufficient energy support for the complex physiological functions of cells. However, traditional culture dishes often find it difficult to accurately control the coordinated supply of nutrient solution and oxygen. In some conventional culture systems, oxygen supply may be uneven, resulting in excessive or low oxygen concentration in local areas. Excessive oxygen concentration may cause oxidative stress and damage stem cells; while too low oxygen concentration will limit the aerobic respiration of cells, affecting their energy metabolism and normal function. At the same time, when supplementing nutrient solution, if the oxygen supply cannot be reasonably and synchronously adjusted, it is easy to cause fluctuations in the culture microenvironment, such as instantaneous changes in local nutrient concentration and obstructed oxygen diffusion. These will interfere with the normal growth and differentiation process of stem cells, reduce the efficiency and quality of stem cell culture, increase culture cost and time, and are not conducive to the development of stem cell research and related applications. Therefore, there is an urgent need for an innovative culture dish that can effectively regulate oxygen supply when providing nutrient solution.

[0004] After searching, it was found that the prior art publication number is CN110438005A, which discloses a stem cell culture dish, which includes: a dish cover, a dish body, a dish cavity, and a base. A dish cover twist is arranged on the top of the dish cover, and the dish cover twist is connected to the dish cover through a dish cover column; iron bars are arranged on both sides of the lower edge of the dish cover; a circular dish cavity is arranged in the dish body, and the upper part of the base is recessed downward for placing the dish body. An electromagnet is arranged at the bottom of the base. By arranging the electromagnet and the iron bar, the movement and falling of the culture dish are prevented, thereby improving the safety of the culture dish.

[0005] Therefore, based on the above search and in combination with the existing ones, when the above scheme is used, it only aligns the dish cover and the dish body to fit tightly, and cannot automatically add nutrient solution to the internal stem cells, nor can it provide air, which has limitations. In order to solve the problems of not being able to automatically add nutrient solution to the internal stem cells and not being able to provide air, we propose a stem cell culture dish. Summary of the invention

[0006] The object of the present invention is to provide a stem cell culture dish to solve the problems raised in the above background technology.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A stem cell culture dish, comprising a mounting base, a culture dish box fixedly connected to the upper end of the mounting base, a culture dish cover clamped on the upper end of the culture dish box, stem cells filled between the culture dish cover and the inner wall of the culture dish box, a mounting cylinder arranged above the mounting base, the mounting cylinder being located directly above the culture dish cover, the mounting cylinder and the culture dish cover being fixedly connected via two limiting telescopic arms, and a liquid storage box arranged at the upper end of the mounting cylinder;

[0009] The liquid storage box is filled with nutrient solution, and an extraction mechanism is fixedly installed between the liquid storage box and the culture dish cover. The extraction mechanism can extract nutrient solution from the liquid storage box and transport it to the culture dish cover and the culture dish box. An oxygen supply mechanism is also fixedly connected between the liquid storage box and the culture dish cover, and the oxygen supply mechanism can provide oxygen to the stem cells inside the culture dish cover and the culture dish box.

[0010] As a further feature of the present invention, the extraction mechanism includes a connecting main pipe, which is fixedly connected to the inner wall of the mounting tube, and a plurality of matching tubes are fixedly connected to the upper end of the connecting main pipe, and a connecting sleeve is also fixedly connected to the upper end of the matching tube. The connecting sleeve can achieve effective connection with multiple matching tubes, and its unique connection structure design can gather and collect the nutrient solution inside each matching tube. When the nutrient solution needs to be discharged, all the collected nutrient solution can be discharged together through the unified discharge channel of the connecting sleeve, thereby greatly improving the convenience and efficiency of nutrient solution discharge.

[0011] As a further feature of the present invention, the upper end of the connecting sleeve is fixedly connected to a connecting tube, and a snap-in ball with a reset function is also arranged in each connecting tube. The bottom of the snap-in ball is also fixedly connected to a first pull rope, and a driving motor is fixedly installed on the bottom end of the inner wall of the mounting tube. When the snap-in ball is pulled by the external force applied by the first pull rope, the snap-in ball will overcome the resistance it encounters and shift its position accordingly. When the pulling force of the first pull rope disappears, the snap-in ball can accurately and automatically return to its original initial position so as to participate in related mechanical operations or connection coordination and other functional links again.

[0012] As a further feature of the present invention, the output end of the driving motor is fixedly connected to a recovery wheel, a second pull rope is wound around the outer wall of the recovery wheel, and the free end of the second pull rope is also fixedly connected to all the first pull ropes. The recovery wheel can apply traction to the second pull ropes during operation to displace the second pull ropes, and the second pull ropes are interconnected with all the first pull ropes. By virtue of this connection relationship, when the second pull rope is pulled by the recovery wheel, it will transmit this pulling force to each first pull rope, thereby driving all the first pull ropes to perform corresponding pulling actions synchronously, thereby realizing specific mechanical linkage or control functions.

[0013] As a further feature of the present invention, a drip tube is fixedly connected to the inner wall of the culture dish cover, and the connecting main pipe is connected to the drip tube. A contact circular plate with a reset function is also provided on the inner wall of the drip tube. The upper end of the contact circular plate is in contact with the bottom of the drip tube. The contact circular plate has a unique reset function. When pulled by an external force, it will change its original position accordingly. However, after the pulling force is removed, the contact circular plate can automatically and accurately return to its initial position and state by virtue of its own restoring force, thereby ensuring its cyclic stability and reliability during the operation of the entire mechanical device or system.

[0014] As a further feature of the present invention, the oxygen supply mechanism includes a movable circular plate, which is slidably connected to the outer wall of the mating tube, and a first folding tube is fixedly connected between the movable circular plate and the mounting cylinder, and a telescopic rod is fixedly connected between the movable circular plate and the mounting cylinder. The first folding tube can realize a folding and contraction function, and the degree of contraction depends on the distance between the movable circular plate and the mounting cylinder. When the distance between the movable circular plate and the mounting cylinder changes, the first folding tube will fold or stretch accordingly according to the change in the distance, thereby flexibly adapting to different space requirements or mechanical operation requirements, thereby ensuring the coordination and effectiveness of the entire device system.

[0015] As a further feature of the present invention, a second elastic telescopic arm is fixedly connected to the bottom of the liquid storage box, a first air vent is opened inside the movable circular plate, and the outer wall of the second elastic telescopic arm is penetrated through the first air vent; a blocking plate is fixedly connected to the bottom of the second elastic telescopic arm, the first air vent is connected to the first folding tube, and a second folding tube with a reset function is fixedly connected to the upper end of the mounting base, the second folding tube is connected to the first folding tube through a rubber tube, and since the second folding tube and the first folding tube are connected by the rubber tube, when the distance between the two changes, the rubber tube can adaptively contract or extend by virtue of its own elastic properties, thereby achieving effective pulling and connection between the two, and ensuring the stability and functionality of the entire connection structure under different working conditions.

[0016] As a further feature of the present invention, a damper is fixedly connected to the inner wall of the second folding tube, and an exhaust pipe is fixedly connected to the outer wall of the second folding tube. Because the damper is fixed to the inner wall of the second folding tube, when the second folding tube expands and then begins to contract, the damper intervenes with its damping characteristics to hinder the recovery process of the second folding tube, thereby effectively slowing down the recovery speed of the second folding tube, making the entire contraction process smoother and more controllable, and avoiding structural shock or instability that may be caused by rapid recovery.

[0017] As a further feature of the present invention, the outer wall of the culture dish cover is provided with two second air holes, and the exhaust pipe and the corresponding second air holes are attracted and connected to each other by magnets. An electronic valve is installed inside the exhaust pipe, and the electronic valve can flexibly and accurately regulate the air circulation in the exhaust pipe. The exhaust pipe can be opened and closed independently according to preset program instructions or real-time system feedback information, thereby achieving effective management and optimized control of related equipment.

[0018] As a further feature of the present invention, a third pulling rope is wound around the outer wall of the recovery wheel, and a free end of the third pulling rope is fixedly connected to the bottom of the movable circular plate, so that the recovery wheel can freely pull the third pulling rope.

[0019] Beneficial Effects

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. It can automatically add nutrient solution between the culture dish box and the culture dish cover without manual intervention. This device effectively avoids the problems of dosage error, operation contamination and unstable time interval caused by manual addition, creates a more constant and suitable growth microenvironment for stem cells, greatly improves the accuracy, reliability and efficiency of stem cell culture, and strongly promotes the further development of stem cell-related research and application;

[0022] 2. In addition to providing nutrient solution to stem cells, air will also be continuously provided for a long time to ensure that on the basis of continuously providing cells with rich nutrients to support their key physiological processes such as growth, proliferation and differentiation, a stable air supply provides sufficient oxygen source for cell aerobic respiration, so that energy metabolism in cells can proceed smoothly, maintain the balance of gas exchange and material metabolism in the stem cell culture environment, effectively guarantee the activity and functional state of stem cells under in vitro culture conditions, and provide more reliable and efficient culture conditions support for stem cell-related research and applications; BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a front view of a stem cell culture dish;

[0024] Figure 2 This is a schematic diagram of the internal structure of a liquid storage box of a stem cell culture dish;

[0025] Figure 3 This is a schematic diagram of the internal structure of a mounting tube of a stem cell culture dish;

[0026] Figure 4 It is a schematic diagram of the position structure of a limited telescopic arm of a stem cell culture dish;

[0027] Figure 5 for Figure 4 The enlarged schematic diagram of point B in the middle;

[0028] Figure 6 A schematic diagram of the internal structure of a first folding tube of a stem cell culture dish;

[0029] Figure 7 for Figure 6 Schematic diagram at A in the middle;

[0030] Figure 8 A schematic diagram of the position structure of the connecting tubes of a stem cell culture dish;

[0031] Fig. 9 This is a schematic diagram of the internal structure of the second folding tube of a stem cell culture dish.

[0032] In the figure: 1, mounting base; 2, suction cup; 3, mounting tube; 4, culture dish box; 5, liquid storage box; 6, first elastic telescopic arm; 7, abutment plate; 8, elastic rope; 9, snap-on ball; 10, connecting tube; 11, matching tube; 12, protective shell; 13, first folding tube;

[0033] 14. Connecting sleeve; 15. Petri dish cover; 16. Connecting main pipe; 17. First pulling rope; 18. Second pulling rope; 19. Recovery wheel; 20. Third pulling rope; 21. Moving circular plate; 22. Telescopic rod; 23. First spring; 24. Second elastic telescopic arm; 25. Blocking plate; 26. First air vent; 27. Driving motor; 28. Second air vent; 29. ​​Exhaust pipe; 30. Second folding pipe;

[0034] 31. Second spring; 32. Damper; 33. Position-limiting telescopic arm; 34. Third folding tube; 35. Dropping tube; 36. Third elastic telescopic arm; 37. Abutting circular plate; 101. Extraction mechanism; 201. Oxygen supply mechanism. DETAILED DESCRIPTION

[0035] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0036] Example 1: Please refer to Figure 1 , Figure 2 As shown, a stem cell culture dish comprises a mounting base 1, a culture dish box 4 is fixedly connected to the upper end of the mounting base 1, a culture dish cover 15 is clamped on the upper end of the culture dish box 4, stem cells are filled between the culture dish cover 15 and the inner wall of the culture dish box 4, and the stem cells include: embryonic stem cells, hematopoietic stem cells, mesenchymal stem cells, etc.; the culture dish cover 15 and the culture dish box 4 are both made of glass material, and the glass material has high transparency, which helps the staff to better observe the stem cell cultivation state inside the culture dish cover 15 and the culture dish box 4, and the glass material also has good corrosion resistance and is durable. A mounting cylinder 3 is arranged above the mounting base 1, and the mounting cylinder 3 is located directly above the culture dish cover 15. The mounting cylinder 3 and the culture dish cover 15 are fixedly connected by two limiting telescopic arms 33, and the two limiting telescopic arms 33 are symmetrically distributed between the mounting cylinder 3 and the culture dish cover 15. A liquid storage box 5 is arranged at the upper end of the mounting cylinder 3, and the liquid storage box 5 is filled with nutrient solution, and the nutrient solution is a mixture of amino acids, glucose and vitamins.

[0037] An extraction mechanism 101 is fixedly installed between the liquid storage box 5 and the culture dish cover 15. The extraction mechanism 101 can extract nutrient solution from the inside of the liquid storage box 5 and transport it to the inside of the culture dish cover 15 and the culture dish box 4. An oxygen supply mechanism 201 is also fixedly connected between the liquid storage box 5 and the culture dish cover 15. The oxygen supply mechanism 201 can provide oxygen to the stem cells inside the culture dish cover 15 and the culture dish box 4. The upper end of the mounting base 1 is also abutted against a protective shell 12, and a control chip is also fixedly connected to the inside of the protective shell 12. The outer wall of the protective shell 12 is also provided with two observation ports, and the staff can observe the conditions of the culture dish cover 15 and the culture dish box 4 inside the protective shell 12 through the observation ports.

[0038] Specifically, a first elastic telescopic arm 6 is fixedly connected to the top of the inner wall of the liquid storage box 5, and a contact plate 7 is fixedly connected to the bottom of the first elastic telescopic arm 6, and the outer wall of the contact plate 7 contacts the inner wall of the liquid storage box 5. When the first elastic telescopic arm 6 drives the contact plate 7 to move downward, the contact plate 7 will push the nutrient solution inside the liquid storage box 5 into the extraction mechanism 101. A plurality of suction cups 2 are fixedly connected to the bottom of the mounting base 1, and the plurality of suction cups 2 are distributed in a circle at the bottom of the mounting base 1. The suction cups 2 can be adsorbed on a flat tabletop to enhance the stability of the mounting base 1.

[0039] Example 2: Please refer to Figure 2 - Figure 5 As shown, the extraction mechanism 101 includes a connecting main pipe 16, which is fixedly connected to the inner wall of the installation tube 3. The upper end of the connecting main pipe 16 is fixedly connected to a plurality of matching tubes 11, and the plurality of matching tubes 11 are circumferentially distributed at the upper end of the connecting main pipe 16. The upper end of the matching tube 11 is also fixedly connected to a connecting sleeve 14, which is funnel-shaped. The upper end of the connecting sleeve 14 is fixedly connected to a connecting tube 10. A card ball 9 with a reset function is also arranged in each connecting tube 10, and the card ball 9 is spherical, and the card ball 9 is made of rubber, and the diameter of the cross section of the card ball 9 is larger than the diameter of the matching tube 11. When the card ball 9 moves toward the matching tube When the card ball 9 moves in the inner wall of the matching tube 11, since the card ball 9 is made of rubber, when the card ball 9 enters the inside of the matching tube 11, the card ball 9 is squeezed and contracted, and the card ball 9 will squeeze and transport the nutrient solution in the matching tube 11 to the inside of the connecting main pipe 16. The card ball 9 is fixedly connected to the top of the inner wall of the connecting tube 10 by an elastic rope 8, and the elastic rope 8 is also made of rubber. When the card ball 9 moves, the elastic rope 8 will be stretched and stored, and the elastic rope 8 will also drive the card ball 9 to reset. It is worth noting that the deformation elastic force generated by the card ball 9 is smaller than the elastic force when the elastic rope 8 is stretched, thereby preventing the card ball 9 from being stuck in the matching tube 11.

[0040] The bottom of the clamping ball 9 is also fixedly connected to a first pulling rope 17, and the outer wall of the first pulling rope 17 is passed through the inside of the connecting sleeve 14 and the matching tube 11. The bottom end of the inner wall of the mounting tube 3 is fixedly installed with a driving motor 27, and the output end of the driving motor 27 is fixedly connected to a recovery wheel 19. The outer wall of the recovery wheel 19 is wound with a second pulling rope 18, and the outer wall of the second pulling rope 18 is passed through the inside of the connecting main pipe 16, and the free end of the second pulling rope 18 is also fixedly connected to all the first pulling ropes 17. The top of the inner wall of the culture dish cover 15 is fixedly connected with a drip tube 35, and the connecting The main connecting pipe 16 and the dripping pipe 35 are connected to each other, and the main connecting pipe 16 and the dripping pipe 35 are fixedly connected through the third folding pipe 34, and the third folding pipe 34 is also made of rubber material. The third folding pipe 34 can be freely extended and retracted as the mounting tube 3 moves up and down. The inner wall of the dripping pipe 35 is also provided with an abutting circular plate 37 with a reset function. The abutting circular plate 37 and the inner wall of the dripping pipe 35 are fixedly connected by a third elastic telescopic arm 36. The third elastic telescopic arm 36 can drive the abutting circular plate 37 to quickly reset, and the upper end of the abutting circular plate 37 abuts against the bottom of the dripping pipe 35.

[0041] See also Figure 2 , Figure 3 , Figure 6 - Fig. 9 As shown, the oxygen supply mechanism 201 includes a movable circular plate 21, which is slidably connected to the outer wall of the matching tube 11. Specifically, a plurality of sliding openings are provided inside the movable circular plate 21, and the outer wall of each matching tube 11 is slidably connected to the inner wall of the corresponding sliding opening. The outer wall of the matching tube 11 and the inner wall of the sliding opening are both coated with lubricating oil. When the movable circular plate 21 slides on the outer wall of the matching tube 11, the matching tube 11 can limit the movable circular plate 21 through the sliding opening, and also has a guiding function, thereby improving the stability of the movable circular plate 21 when moving. The lubricating oil can also reduce The friction between the matching tube 11 and the sliding port prolongs the service life of the matching tube 11. The first folding tube 13 is also fixedly connected between the moving circular plate 21 and the mounting tube 3, and the telescopic rod 22 and the first spring 23 are also fixedly connected between the moving circular plate 21 and the mounting tube 3. The first spring 23 is sleeved on the outer wall of the telescopic rod 22. The outer wall of the recovery wheel 19 is also wound with a third pulling rope 20, and the free end of the third pulling rope 20 is fixedly connected to the bottom of the moving circular plate 21. The telescopic rod 22 and the first spring 23 are both located inside the first folding tube 13.

[0042] A second elastic telescopic arm 24 is fixedly connected to the bottom of the liquid storage box 5, a first air vent 26 is opened inside the movable circular plate 21, and the outer wall of the second elastic telescopic arm 24 is penetrated inside the first air vent 26, a blocking plate 25 is fixedly connected to the bottom of the second elastic telescopic arm 24, the diameter of the blocking plate 25 is larger than the aperture inside the first air vent 26, the first air vent 26 is connected to the first folding tube 13, and a second folding tube 30 with a reset function is fixedly connected to the upper end of the mounting base 1, and a second spring 31 is fixedly connected inside the second folding tube 30, and the second spring 31 can drive the second folding tube 30 to quickly reset, the second folding tube 30 is connected to the first folding tube 13 through a rubber tube, and the inner wall of the second folding tube 30 is also fixedly connected to a damper 32, the outer wall of the second folding tube 30 is also fixedly connected to an exhaust pipe 29, and the outer wall of the culture dish cover 15 is also provided with two second air holes 28, and magnets are pasted on the bottom of the exhaust pipe 29 and the inner wall of one of the second air holes 28, the exhaust pipe 29 and the corresponding second air hole 28 are attracted and abutted against each other through the magnet, and an electronic valve is installed inside the exhaust pipe 29.

[0043] Specifically, when the second foldable tube 30 is filled with gas, the second foldable tube 30 will expand and stretch. At this time, the second foldable tube 30 will be filled with air. When the second foldable tube 30 is expanded, the second spring 31 will store force and extend, and the damper 32 will slow down the expansion speed of the second foldable tube 30. When the second foldable tube 30 needs to release air, the second spring 31 will release the stored force, and the second spring 31 will drive the second foldable tube 30 to reset and contract. Since the damper 32 will slow down the contraction speed of the second foldable tube 30, the air inside the second foldable tube 30 is discharged from the exhaust pipe 29 very slowly, and since the exhaust pipe 29 will be horizontally aligned with the corresponding second air vent 28, when the exhaust pipe 29 discharges gas, the air will pass through the upper end of the second air vent 28. Since the diameter of the second air vent 28 is small, the air discharged from the exhaust pipe 29 will be in a high-speed flow state. According to the principles of fluid mechanics, the high-speed flowing gas (the gas ejected from the exhaust pipe 29) will form a low-pressure area around it. This is because when the flow rate of the gas increases, its pressure will decrease (Bernoulli principle). Due to the pressure difference between the inside and outside of the second air vent 28, the air between the culture dish cover 15 and the inside of the culture dish box 4 will flow from the high-pressure area (the inside of the culture dish cover 15 and the culture dish box 4) to the low-pressure area (near the outer wall of the second air vent 28) under the action of this pressure difference, thereby driving the air flow between the culture dish cover 15 and the inside of the culture dish box 4. At this time, the second air vent 28 at the bottom of the exhaust pipe 29 is in an air outlet state, and the other second air vent 28 is in an air intake state. At this time, the air flowing inside the culture dish cover 15 and the culture dish box 4 is small and will not affect the growth of the internal stem cells, so an aerobic environment can be provided for the stem cells.

[0044] The working principle of the present invention is: when in use, it is only necessary to start the driving motor 27, and the driving motor 27 drives the recovery wheel 19 to rotate slowly and uniformly, and the recovery wheel 19 recovers and winds the second pulling rope 18 and the third pulling rope 20. When the second pulling rope 18 is pulled, the second pulling rope 18 will also pull all the first pulling ropes 17, and the first pulling rope 17 will drive the card receiving ball 9 to move slowly. When the card receiving ball 9 enters the inside of the matching tube 11, the nutrient solution inside the matching tube 11 will be squeezed into the inside of the connecting main pipe 16 by the card receiving ball 9, and the nutrient solution inside the connecting main pipe 16 will be squeezed into the inside of the third folding tube 34 and the dripping tube 35 by the card receiving ball 9. As the pressure inside the dripping tube 35 increases, the abutting circular plate 37 will be pushed open. It is worth noting that, since the descending speed of the card receiving ball 9 is uniform and slow, the nutrient solution discharged from the dripping tube 35 will be squeezed out little by little to prevent the nutrient solution from directly damaging the stem cells.

[0045] At the same time, when the recovery wheel 19 rotates, the third pulling rope 20 will also be pulled. When the third pulling rope 20 moves downward, the blocking plate 25 will abut against the first air vent 26 to block the first air vent 26, and the second elastic telescopic arm 24 will be extended. At this time, the gas inside the first folding tube 13 will enter the second folding tube 30. When the nutrient solution is supplied, the recovery wheel 19 stops rotating, the elastic rope 8 will drive the card receiving ball 9 to reset, and the first spring 23 will drive the moving circular plate 21 to reset. When the moving circular plate 21 is reset, 27 will stop driving 19 to rotate, and the moving circular plate 21 will drive the recovery wheel 19 to rotate in the opposite direction through the third pulling rope 20. When the moving circular plate 21 is reset, After the plate 21 is reset, the blocking plate 25 will be separated from the contact with the first air vent 26. It is worth noting that the negative pressure generated inside the first folding tube 13 when it is reset is smaller than the elasticity of the second elastic telescopic arm 24. Therefore, when the first folding tube 13 is reset, the internal negative pressure will not tightly adsorb the blocking plate 25 on the outer wall. When the second folding tube 30 is not filled with air, the gas inside the second folding tube 30 will be discharged from the exhaust pipe 29. A second air vent 28 at the upper end of the culture dish cover 15 will generate a pressure difference. At this time, the air inside the culture dish cover 15 and the culture dish box 4 will be in a flowing state. When the second folding tube 30 is completely reset, the nutrient solution can be added for the second time.

[0046] The mounting tube 3 and the culture dish cover 15 can be directly moved upwards, so that the culture dish cover 15 and the culture dish box 4 are disengaged from each other, so that new stem cells can be added. When the second folding tube 30 expands, the electronic valve will be closed to prevent the air inside the second folding tube 30 from being discharged. When the movable circular plate 21 is reset, the electronic valve will open and the air inside the second folding tube 30 will be discharged.

[0047] The above are only preferred specific implementation modes of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed in the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A stem cell culture dish, comprising a mounting base (1), characterized in that: The upper end of the mounting base (1) is fixedly connected to a culture dish box (4), the upper end of the culture dish box (4) is engaged with a culture dish cover (15), and stem cells are filled between the culture dish cover (15) and the inner wall of the culture dish box (4). A mounting tube (3) is arranged above the mounting base (1), and the mounting tube (3) is located directly above the culture dish cover (15). The mounting tube (3) and the culture dish cover (15) are fixedly connected via two limiting telescopic arms (33), and a liquid storage box (5) is arranged at the upper end of the mounting tube (3); The liquid storage box (5) is filled with nutrient solution. An extraction mechanism (101) is fixedly installed between the liquid storage box (5) and the culture dish cover (15). The extraction mechanism (101) can extract nutrient solution from the liquid storage box (5) and transport it to the culture dish cover (15) and the culture dish box (4). An oxygen supply mechanism (201) is also fixedly connected between the liquid storage box (5) and the culture dish cover (15). The oxygen supply mechanism (201) can provide oxygen to the stem cells in the culture dish cover (15) and the culture dish box (4).

2. A stem cell culture dish according to claim 1, characterized in that: The extraction mechanism (101) comprises a connecting main pipe (16), the connecting main pipe (16) is fixedly connected to the inner wall of the mounting tube (3), the upper end of the connecting main pipe (16) is fixedly connected to a plurality of matching pipes (11), and the upper end of the matching pipe (11) is also fixedly connected to a connecting sleeve (14).

3. A stem cell culture dish according to claim 2, characterized in that: The upper end of the connecting sleeve (14) is fixedly connected to a connecting tube (10), and a clamping ball (9) with a reset function is also arranged in each connecting tube (10). The bottom of the clamping ball (9) is also fixedly connected to a first pulling rope (17), and a driving motor (27) is fixedly installed at the bottom end of the inner wall of the installation tube (3).

4. A stem cell culture dish according to claim 3, characterized in that: The output end of the driving motor (27) is fixedly connected to a recovery wheel (19), the outer wall of the recovery wheel (19) is wound with a second pulling rope (18), and the free end of the second pulling rope (18) is also fixedly connected to all the first pulling ropes (17).

5. A stem cell culture dish according to claim 4, characterized in that: The inner wall of the culture dish cover (15) is fixedly connected with a dripping tube (35), and the connecting main pipe (16) and the dripping tube (35) are communicated with each other. The inner wall of the dripping tube (35) is also provided with an abutting circular plate (37) with a reset function, and the upper end of the abutting circular plate (37) abuts against the bottom of the dripping tube (35).

6. The stem cell culture dish according to claim 1, characterized in that: The oxygen supply mechanism (201) comprises a movable circular plate (21), the movable circular plate (21) being slidably connected to the outer wall of the matching tube (11), a first folding tube (13) being fixedly connected between the movable circular plate (21) and the mounting tube (3), and a telescopic rod (22) being fixedly connected between the movable circular plate (21) and the mounting tube (3).

7. A stem cell culture dish according to claim 6, characterized in that: A second elastic telescopic arm (24) is fixedly connected to the bottom of the liquid storage box (5); a first air vent (26) is provided inside the movable circular plate (21), and the outer wall of the second elastic telescopic arm (24) is passed through the first air vent (26); a blocking plate (25) is fixedly connected to the bottom of the second elastic telescopic arm (24); the first air vent (26) is connected to the first folding tube (13); a second folding tube (30) with a reset function is fixedly connected to the upper end of the mounting base (1); and the second folding tube (30) is connected to the first folding tube (13) through a rubber tube.

8. A stem cell culture dish according to claim 7, characterized in that: The inner wall of the second folded tube (30) is also fixedly connected to a damper (32), and the outer wall of the second folded tube (30) is also fixedly connected to an exhaust pipe (29).

9. A stem cell culture dish according to claim 8, characterized in that: The outer wall of the culture dish cover (15) is also provided with two second air holes (28); the exhaust pipe (29) and the corresponding second air holes (28) are attracted and abutted against each other by a magnet; and an electronic valve is installed inside the exhaust pipe (29).

10. The stem cell culture dish according to claim 4, characterized in that: A third pulling rope (20) is also wound around the outer wall of the recovery wheel (19), and the free end of the third pulling rope (20) is fixedly connected to the bottom of the movable circular plate (21).

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