Amplification analyzer and detection system

Abstract

The invention discloses an amplification analyzer and a detection system. The amplification analyzer comprises a processor, a memory connected with the processor, a shell, a scheduling mechanism, an amplification mechanism and a signal analysis mechanism, the processor is configured to determine a working mode corresponding to an instruction according to the instruction of a user, determine a first placement position of a consumable to be amplified and analyzed and / or determine a second placement position of the consumable subjected to amplification and analysis according to the corresponding working mode, and select the consumable to be amplified and analyzed by selecting the different first placement position and second placement position. The amplification analyzer not only can complete stand-alone analysis of consumables, but also can be cascaded with other equipment under the cooperation of the transfer window and the transfer mechanism, so that the use scene of the amplification analyzer is expanded, and the use flexibility of the amplification analyzer is improved.

Description

Technical Field

[0001] The present application relates to the technical field of PCR detection, and in particular to an amplification analyzer and a detection system. Background Art

[0002] Digital polymerase chain reaction (Digital PRC) is a new method for nucleic acid detection and quantitative analysis. Its core principle is to divide the consumables to be tested into numerous tiny reaction partitions, perform independent PCR amplification in each partition, and finally calculate the concentration of the target by counting the number of positive and negative partitions, thereby realizing nucleic acid detection and analysis.

[0003] Amplification analyzers are an important part of digital PCR testing. Their main function is to amplify and analyze the segmented tiny reaction partitions, thereby achieving high sensitivity and accurate quantitative detection of nucleic acids. However, with the increase in detection scenarios, traditional amplification analyzers have long been unable to meet the growing detection needs. Summary of the invention

[0004] The purpose of this application is to provide an amplification analyzer and a detection system, aiming to solve the problem in the related art that the amplification analyzer has poor scalability and is difficult to meet the detection needs of multiple scenarios.

[0005] To achieve the purpose of the present application, in a first aspect, the present application provides an amplification analyzer, comprising a processor, a memory connected to the processor, a shell, a scheduling mechanism, an amplification mechanism and a signal analysis mechanism; the shell comprises an installation cavity and at least one transfer window, the installation cavity having a consumable loading position for loading consumables to be amplified and analyzed and a waste placement position for placing consumables that have completed amplification and analysis; the transfer window is used to cooperate with the transfer mechanism to transfer the consumables to be amplified and analyzed into the installation cavity and / or transfer the consumables that have completed amplification and analysis out of the installation cavity, the transfer mechanism having a forward transfer position for transferring the consumables to be amplified and analyzed into the installation cavity and / or a backward transfer position for transferring the consumables that have completed amplification and analysis out of the installation cavity; the amplification mechanism, the analysis mechanism and the scheduling mechanism are arranged in the installation cavity, and are all controlled by the processor;

[0006] The memory is used to store at least two working modes for the processor to call respectively;

[0007] The processor is configured to determine, according to the user's instruction, a working mode corresponding to the instruction, determine, according to the corresponding working mode, a first placement position for consumables to be amplified and analyzed, and / or determine a second placement position for consumables that have completed amplification and analysis, and control the scheduling mechanism to extract the consumables to be amplified and analyzed from the first placement position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the second placement position;

[0008] Among them, the first placement position includes the consumables loading position or the forward transfer position; the second placement position includes the waste placement position or the backward transfer position.

[0009] In a possible implementation, the working mode includes a stand-alone mode;

[0010] In the stand-alone mode, the first placement position is a consumable loading position, and the second placement position is a waste placement position;

[0011] The processor is configured to control the scheduling mechanism to extract the consumables to be amplified and analyzed from the consumable loading position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the waste placement position.

[0012] In a possible implementation, the working mode also includes a first online mode; a forward transport mechanism is provided in the installation cavity, the forward transport mechanism is used to connect with the front cascade device, and the forward transport mechanism is controlled by the processor, or by the processor of the front cascade device, or by the processor of the amplification analyzer and the host computer of the front cascade device;

[0013] In the first online mode, the first placement position is a forward transfer position, and the second placement position is a waste placement position;

[0014] The processor is configured to control the scheduling mechanism to extract the consumables to be amplified and analyzed in the forward transfer position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism to determine and output the corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the waste placement position.

[0015] In a possible implementation, the amplification analyzer further includes a consumables cache location, and the consumables cache location is used to store the consumables to be amplified and analyzed that are transported by the forward transport mechanism;

[0016] The processor is configured to control the scheduling mechanism to transfer the consumables to be amplified and analyzed on the forward transfer position to the consumables cache position according to preset scheduling rules or user instructions when the amplification and analysis mechanism is in a non-idle state; and to control the scheduling mechanism to transfer the consumables to be amplified and analyzed on the consumables cache position to the amplification mechanism for amplification when the amplification and analysis mechanism is in an idle state according to preset scheduling rules or user instructions, and then transfer them to the signal analysis mechanism to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the waste placement position.

[0017] In a possible implementation, the working mode also includes a second online mode; a backward transport mechanism is provided in the installation cavity, the backward transport mechanism is used to connect with the post-amplification analyzer, and the transport mechanism is controlled by the processor, or by the post-amplification analyzer, or by the processor of the amplification analyzer and the host computer of the post-amplification analyzer;

[0018] In the second online mode, the first placement position is a consumable loading position, and the second placement position is a backward transfer position;

[0019] The processor is configured to control the scheduling mechanism to extract the consumables to be amplified and analyzed from the consumable loading position, and transfer them to the backward transfer position, so as to transport them to the post-amplification analyzer through the backward transfer mechanism.

[0020] In a possible implementation, the working mode also includes a third online mode, the transfer window includes a forward transfer window and a backward transfer window; a transfer mechanism is arranged in the installation cavity, the transfer mechanism includes a forward transfer mechanism and a backward transfer mechanism, the forward transfer mechanism extends into the installation cavity through the forward transfer window, and the forward transfer position is arranged in the forward transfer mechanism; the backward transfer mechanism extends out of the installation cavity through the backward transfer window; the backward transfer position is arranged in the backward transfer mechanism; the forward transfer mechanism is used to connect with the front cascade device, and the forward transfer mechanism is controlled by the processor, or by the processor of the front cascade device, or by the processor of the upper computer of the amplification analyzer and the front cascade device; the backward transfer mechanism is used to connect with the rear amplification analyzer, and the transfer mechanism is controlled by the processor, or by the processor of the rear amplification analyzer, or by the processor of the amplification analyzer and the upper computer of the rear amplification analyzer;

[0021] In the third online mode, the first placement position is a forward transfer position, and the second placement position is a backward transfer position;

[0022] The processor is configured to control the dispatch mechanism to extract the consumables to be amplified and analyzed in the forward transfer position and transfer them to the backward transfer position so as to transport them to the post-amplification analyzer through the backward transfer mechanism, and / or

[0023] The processor is configured to control the scheduling mechanism to extract the consumables to be amplified and analyzed in the forward transfer position and transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the waste placement position.

[0024] In one possible implementation, the processor is configured to, when the amplification mechanism is in a non-idle state, control the scheduling mechanism to extract the consumables to be amplified and transfer them to the backward transfer position, either according to preset scheduling conditions or according to user instructions.

[0025] In a possible implementation, the amplification analyzer further includes a consumables cache location, and the consumables cache location is used to store the consumables to be amplified and analyzed that are transported by the forward transport mechanism;

[0026] The processor is configured to control the scheduling mechanism to transfer the consumables to be amplified and analyzed on the forward transfer position to the consumable cache position, and when the backward transfer position is idle, control the scheduling mechanism to transfer the consumables to be amplified and analyzed on the consumable cache position to the backward transfer position according to preset scheduling rules.

[0027] In a possible implementation manner, the consumable buffer position is multiplexed with the consumable loading position structure.

[0028] In a second aspect, the present application further proposes a detection system, which includes a cascade device and an amplification analyzer; at least one of the cascade device and the amplification analyzer is provided with the transfer mechanism; the transfer mechanism is connected between the amplification analyzer and the cascade device and passes through the transfer window; the transfer mechanism is used to transfer the consumables in the amplification analyzer and the cascade device; the amplification analyzer includes a processor, a memory connected to the processor, a shell, a scheduling mechanism, an amplification mechanism and a signal analysis mechanism; the shell includes an installation cavity and at least one transfer window, and the installation cavity has a The consumable loading position is used for loading consumables to be amplified and analyzed, and the waste placement position is used for placing consumables that have completed amplification and analysis; the transfer window is used to cooperate with the transfer mechanism to transfer the consumables to be amplified and analyzed into the installation cavity and / or transfer the consumables that have completed amplification and analysis out of the installation cavity, and the transfer mechanism has a forward transfer position for transferring the consumables to be amplified and analyzed into the installation cavity and / or a backward transfer position for transferring the consumables that have completed amplification and analysis out of the installation cavity; the amplification mechanism, the analysis mechanism and the scheduling mechanism are arranged in the installation cavity, and are all controlled by the processor;

[0029] The memory is used to store at least two working modes for the processor to call respectively;

[0030] The processor is configured to determine, according to the user's instruction, a working mode corresponding to the instruction, determine, according to the corresponding working mode, a first placement position for consumables to be amplified and analyzed, and / or determine a second placement position for consumables that have completed amplification and analysis, and control the scheduling mechanism to extract the consumables to be amplified and analyzed from the first placement position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the second placement position;

[0031] Among them, the first placement position includes the consumables loading position or the forward transfer position; the second placement position includes the waste placement position or the backward transfer position.

[0032] The amplification analyzer of the embodiment of the present application can select different working modes according to different scene requirements, and determine the corresponding first placement position and second placement position according to the different working modes. The processor can control the scheduling mechanism to extract the consumables from the first placement position and place them on the second placement position. By selecting different first placement positions and second placement positions, the amplification analyzer can not only complete the single-machine analysis of consumables, but also can be cascaded with other devices with the cooperation of the transfer window and the transfer mechanism, so as to expand the use scenarios of the amplification analyzer and improve the flexibility of the use of the amplification analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the implementation methods of the present application or the technical solutions in the prior art, the drawings required for use in the implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some implementation methods of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0034] Figure 1 A schematic diagram of the structure of an embodiment of the detection system provided by the present application;

[0035] Figure 2 for Figure 1 A schematic diagram of the three-dimensional structure of an embodiment of an amplification analyzer;

[0036] Figure 3 for Figure 1 Schematic diagram of the internal structure;

[0037] Figure 4 for Figure 3 A schematic diagram of the structure of an embodiment of a scheduling mechanism grabbing a PCR tube plate;

[0038] Figure 5 for Figure 1 Schematic diagram of the operating environment of the amplification analyzer;

[0039] Figure 6 for Figure 1 Schematic diagram of the structure of the connection between the amplification analyzer and the front cascade device;

[0040] Figure 7 for Figure 1 Schematic diagram of the structure of the splicing of the mid-amplification analyzer and the post-amplification analyzer;

[0041] Figure 8 for Figure 1 A schematic diagram of the structure in which the middle amplification analyzer is simultaneously connected with the front cascade device and the rear amplification analyzer;

[0042] Fig. 9 for Figure 1 A schematic diagram of the structure of the amplification analyzer when operating alone;

[0043] Fig.10 for Figure 1 Schematic diagram of the structure of the intermediate transfer mechanism;

[0044] Fig.11 for Fig.10 A schematic structural diagram of an embodiment of a mobile platform;

[0045] Fig.12 for Fig.10 A cross-sectional view of

[0046] Fig.13 for Fig.10 Schematic diagram of the structure of the middle rotating seat at the beginning of reversing;

[0047] Fig.14 for Fig.10 Schematic diagram of the structure of the middle rotating seat during the reversing process;

[0048] Fig.15 for Fig.10 Schematic diagram of the structure after the middle rotating seat has been commutated;

[0049] Fig.16 A schematic diagram of the control flow of the first embodiment of the amplification analyzer provided in the embodiments of the present application;

[0050] Fig.17 A schematic diagram of a flow chart of a processor determining a first placement position and a second placement position according to an operating mode;

[0051] Fig.18 This is a schematic diagram of the control flow of the amplification analyzer provided in an embodiment of the present application in the third online mode.

[0052] Description of reference numerals:

[0053] 1000-amplification analyzer;

[0054] 1-housing, 11-installation cavity, 111a-consumable material loading position, 111b-waste material placement position, 111c-consumable material buffer position, 12-transfer window, 12a-forward transfer window, 12b-rearward transfer window, 13-loading window;

[0055] 2-dispatching mechanism, 2a-mechanical gripper;

[0056] 3- Amplification mechanism;

[0057] 4-transfer mechanism, 41a-forward transfer position, 41b-backward transfer position, 4a-forward transfer mechanism, 4b-backward transfer mechanism, 42-guide rail, 43-moving platform, 44-driving assembly, 431-loading seat, 432-rotating seat, 433-reversing assembly, 4331-pushing member, 4332-elastic member, 4333-limiting member;

[0058] 5-Signal analysis agency;

[0059] 6-Liquid circuit mechanism;

[0060] 2000- cascade device, 2000a- pre-cascade device, 2000b- post-amplification analyzer;

[0061] 3000-consumables, 3100-PCR tubes and plates, 3200-gaps;

[0062] 1001 - processor, 1002 - communication bus, 1003 - user interface, 1004 - network interface, 1005 - memory.

[0063] 10000-Detection System. DETAILED DESCRIPTION

[0064] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0065] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or there can be a central component. When a component is said to be "connected to" another component, it can be directly connected to the other component or there can be a central component at the same time.

[0066] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used in this application and in the specification are only for the purpose of describing specific embodiments and are not intended to limit this application. The term "and / or" used in this application includes any and all combinations of one or more of the related listed items.

[0067] In conjunction with the accompanying drawings, some embodiments of the present application are described in detail below. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0068] Please refer to Figure 1This application proposes a detection system 10000, which is used for digital PCR nucleic acid detection. Unlike traditional integrated digital PCR detectors, the detection system 10000 provided in this application introduces a modular concept, splitting the original digital PCR detector's consumables pre-processing, droplet preparation, amplification analysis and other functional components into independent modular units, thereby reducing the possibility of cross-contamination of consumables or products between processes and improving the accuracy of digital PCR nucleic acid detection results. On the other hand, users can also customize or expand the functions and detection throughput of the detection system 10000 by splicing modules to improve the flexibility of nucleic acid detection.

[0069] Specifically, the detection system 10000 includes an amplification analyzer 1000 and a cascade device 2000. The amplification analyzer 1000 is used to amplify and analyze consumables, and the cascade device 2000 is used to be spliced ​​with the amplification analyzer 1000 to assist the amplification analyzer 1000 in completing the detection process required for other digital PCR nucleic acid detection. The cascade device 2000 can be spliced ​​on the front side of the amplification analyzer 1000 to transport the consumables to be amplified and analyzed to the amplification analyzer 1000. The cascade device 2000 can also be spliced ​​on the back side of the amplification analyzer 1000 to assist the amplification analyzer in processing the consumables to be amplified and analyzed, thereby improving the rate of the detection system 10000 for amplification analysis of consumables.

[0070] Please refer to Figure 2 and Figure 3 The amplification analyzer 1000 includes a housing 1, an amplification mechanism 3, a signal analysis mechanism 5, a processor, a memory, and a scheduling mechanism 2. The housing 1 serves as a supporting skeleton of the amplification analyzer 1000 and is used to support and connect various component assemblies of the amplification analyzer 1000.

[0071] The shell 1 is formed with an installation cavity 11, and the amplification mechanism 3, the signal analysis mechanism 5, the processor, the memory and the scheduling mechanism 2 are installed in the installation cavity 11. The shell 1 can be an open frame formed by overlapping only the structural skeleton, or it can be a sealed structure formed by the structural skeleton and the skin, and the present application does not limit this. In one embodiment of the present application, the installation cavity 11 is a closed chamber, and the air pressure in the installation cavity 11 is lower than the air pressure outside the installation cavity 11; in this way, it is ensured that the air in the installation cavity 11 can only flow from the installation cavity 11 to the outside of the installation cavity 11, thereby reducing the possibility of external contaminants of the amplification analyzer 1000 entering the installation cavity 11 and contaminating the consumables 3000.

[0072] In order to further reduce the possibility of contamination of the consumables 3000 to be amplified in the installation cavity 11, in another possible implementation mode of the present application, a high-efficiency air particulate filter (not shown in the figure) is also installed in the installation cavity 11, and the installation cavity 11 and the atmosphere outside the equipment exchange gases through the high-efficiency air particulate filter, thereby reducing the amount of pollutants entering the installation cavity 11 from the external atmosphere and reducing the risk of cross-contamination.

[0073] The dispatching mechanism 2 may be a mechanical gripper 2a, a conveyor belt, or a vacuum suction cup, and this application does not limit this. Figure 4 The dispatching mechanism 2 uses a mechanical gripper 2a. Correspondingly, a notch 3200 is provided on the side of the PCR tube plate 3100 to facilitate the dispatching mechanism 2 to grab it.

[0074] The amplification mechanism 3 is used to perform polymerase chain reaction amplification on the consumable 3000. Specifically, when the consumable 3000 is placed in the amplification mechanism 3, the heating module in the amplification mechanism 3 will heat the consumable 3000 and keep the temperature of the consumable 3000 within a preset denaturation temperature range, such as between 94°C and 98°C, the purpose of which is to separate the double-stranded DNA in the consumable 3000 into a single strand, and this process is called denaturation of the consumable 3000. Afterwards, the cooling module of the amplification mechanism 3 will quickly cool down the temperature of the consumable 3000 and keep the temperature of the consumable 3000 within a preset annealing temperature range, such as between 40°C and 65°C, the purpose of which is to allow the primer of the DNA polymerase to bind to the specific sequence of the target DNA, and this process is called annealing. Then, the amplification mechanism 3 will turn on the heating module again and heat the consumable 3000 to a preset extension temperature, such as 72°C, so that the nucleic acid in the consumable 3000 can synthesize a new DNA chain under the action of the polymerase and the primer. This process is called the extension of the consumable 3000. Finally, the amplification mechanism 3 repeats the above steps in the order of denaturation-annealing-extension until the nucleic acid in the consumable 3000 is fully reacted, thereby completing the amplification of the consumable 3000.

[0075] There may be one or more amplification mechanisms 3, and the present application does not impose any limitation on this. For example, in one embodiment of the present application, three amplification mechanisms 3 are arranged in the installation cavity 11 of the amplification analyzer 1000, and the three amplification mechanisms 3 are arranged at intervals and operate independently, so as to improve the amplification efficiency of the amplification analyzer 1000 for the consumables 3000.

[0076] The signal analysis mechanism 5 is used to detect the consumables after the amplification is completed. Specifically, when the consumables 3000 are transported from the amplification mechanism 3 to the signal analysis mechanism 5 under the transportation of the scheduling mechanism 2, the signal analysis mechanism 5 will use specific fluorescent dyes (such as SYBR Green or TaqMan probes) and other methods to monitor the fluorescent signal in the consumables 3000 in real time and record the changes in signal intensity. Afterwards, the signal analysis mechanism 5 will process and analyze the monitored fluorescent signal, collect the fluorescence data of each cycle and perform background correction, generate an amplification curve, and calculate the Ct value (threshold cycle number) of the target DNA according to the set threshold. By comparing with the standard curve, the signal analysis mechanism 5 can quantitatively analyze the initial concentration of the target DNA in the sample and ultimately generate the detection result of the nucleic acid.

[0077] Please refer to Figure 5 The processor 1001 is used to call computer instructions to control the operation of the scheduling mechanism 2 and the amplification mechanism 3. The memory is connected to the processor 1001, and the memory is used to store computer instructions for the processor 1001 to call.

[0078] In some embodiments, the amplification analyzer 1000 also includes a communication bus 1002, a user interface 1003, and a network interface 1004, and the communication bus 1002 is used to realize the connection communication between these components. The user interface 1003 is mainly used for data interaction by the user, and the user interface 1003 may include a display screen (Disp l ay), an input unit such as a keyboard (Keyboard), and the optional user interface 1003 may also include a standard wired interface and a wireless interface. The network interface 1004 is mainly used for data communication with a network server, and the network interface 1004 may optionally include a standard wired interface and a wireless interface (such as a wireless fidelity (Wi re less-Fidelity, Wi-Fi) interface).

[0079] Please refer to Figure 3 In some implementations, the housing 1 is provided with a transfer window 12 communicating with the installation cavity 11, a transfer mechanism 4 is provided in the installation cavity 11, and the memory stores at least two working modes. The processor 1001 can determine the working mode corresponding to the instruction according to the user's instruction, determine the first placement position of the consumables to be amplified according to the corresponding working mode and the specific working mode, and / or determine the second placement position of the consumables that have completed amplification, and also control the scheduling mechanism 2 to extract the consumables to be amplified in the first placement position, transfer it to the amplification mechanism 3 for amplification, and then transfer it to the signal analysis mechanism 5 for signal analysis to determine and output the corresponding analysis results, and finally transfer the consumables that have completed amplification to the second placement position.

[0080] The amplification analyzer of the embodiment of the present application can select different working modes according to different scene requirements, and determine the corresponding first placement position and second placement position according to different working modes. After the scheduling mechanism extracts the consumables to be amplified and analyzed in the first placement position and transfers them to the amplification mechanism for amplification, it is transferred to the signal analysis mechanism for signal analysis to determine and output the corresponding analysis results, and finally the consumables that have completed amplification are transferred to the second placement position, thereby completing the amplification and analysis of the consumables. By selecting different first placement positions and second placement positions, the amplification analyzer can not only be used as an independent device to amplify and analyze consumables, but can also be cascaded with other devices with the cooperation of the transfer window and the transfer mechanism, so as to expand the use scenarios of the amplification analyzer and improve the flexibility of the use of the amplification analyzer.

[0081] In some embodiments, there are many ways to set the transfer mechanism 4 and the transfer window 12. Figure 6 The transfer window 12 is a forward transfer window 12a, and the transfer mechanism 4 is a forward transfer mechanism 4a. The amplification analyzer 1000 can be connected to the front cascade device 2000a through the forward transfer window 12a. One end of the forward transfer mechanism 4a is set or extended in the installation cavity 11, and the other end passes through the forward transfer window 12a and is set or extended in the front cascade device 2000a. The forward transfer mechanism 4a is used to transfer the consumables 3000 in the front cascade device 2000a to the amplification analyzer 1000.

[0082] Accordingly, a forward transfer position 41a for receiving consumables to be amplified is provided in the installation chamber 11. The forward transfer position 41a and the forward transfer mechanism 4a overlap at least part of the space in the installation chamber 11, and the forward transfer mechanism 4a transfers the consumables 3000 in the front cascade device 2000a to the forward transfer position 41a. In some embodiments, a waste placement position 111b for discarding consumables is provided in the installation chamber 11.

[0083] In some embodiments, the processor 1001 of the amplification analyzer 1000 calls the first online mode stored in the memory 1005. In the first online mode, the processor 1001 sets the forward transfer position 41a to the first placement position and the waste placement position 111b to the second placement position; after the consumable 3000 enters the installation cavity 11, the processor controls the scheduling mechanism 2 to extract the consumable 3000 to be amplified in the forward transfer position 41a, and transfers it to the amplification mechanism 3 for amplification, and then transfers it to the signal analysis mechanism 5 to determine and output the corresponding analysis results, and finally transfers the consumable 3000 that has completed the amplification to the waste placement position 111b, thereby completing the amplification analysis of the consumable 3000.

[0084] It should be noted that the forward transfer mechanism 4a can be pre-set in the amplification analyzer 1000; it can also be pre-set in the pre-cascade device 2000a; it can also be an independent component independent of the amplification analyzer 1000 and the pre-cascade device 2000a, and it is assembled and installed only when the amplification analyzer 1000 and the pre-cascade device 2000a are spliced, and the present application does not impose any restrictions on this.

[0085] In some embodiments, the forward transfer mechanism 4a is controlled by a processor of the amplification analyzer 1000. Specifically, the processor controls the operation of the forward transfer mechanism 4a and obtains the state of the forward transfer mechanism 4a. For example, under the control of the processor of the amplification analyzer 1000, the forward transfer mechanism 4a first transfers the consumables 3000 in the front cascade device 2000a to the forward transfer position 41a in the installation cavity 11 through the forward transfer window 12a, and then controls the scheduling mechanism 2 to extract the consumables 3000 in the forward transfer position 41a. In some embodiments, the forward transfer mechanism 4a is controlled by a processor of the front cascade device 2000a. Specifically, the processor controls the operation of the forward transfer mechanism 4a and obtains the state of the forward transfer mechanism 4a. For example, under the control of the processor of the pre-cascade device 2000a, the forward transport mechanism 4a first transports the consumables 3000 in the pre-cascade device 2000a to the forward transport position 41a of the installation chamber 11 through the forward transport window 12a, and then sends the state information of the forward transport mechanism 4a to the processor of the amplification analyzer 1000 through the direct or indirect (through the host computers of both) information interaction mechanism between the processor of the amplification analyzer 1000 and the processor of the pre-cascade device 2000a, so that the processor of the amplification analyzer 1000 controls the scheduling mechanism 2 to extract the consumables 3000 from the forward transport position 41a after determining that the consumables 3000 have been transported to the forward transport position 41a. In some embodiments, the forward transport mechanism 4a is controlled by the processors of the host computers of the amplification analyzer 1000 and the pre-cascade device 2000a. Specifically, the processor of the host computer directly or indirectly controls the operation of the forward transfer mechanism 4a, obtains the state of the forward transfer mechanism 4a, and based on the state, directly or indirectly controls the processor of the amplification analyzer 1000 to retrieve the consumables 3000 on the forward transfer position 41a.

[0086] In certain embodiments, the pre-cascade device 2000a is a droplet preparation instrument. The droplet preparation instrument can prepare droplets and / or collect droplets to the consumables 3000. That is, in the droplet preparation instrument, the droplet preparation mechanism of the droplet preparation instrument will place the nucleic acid solution to be detected in the droplet chip, and drive the nucleic acid solution to be detected to flow in the microchannel of the droplet chip through the pressure assembly, and form droplets. After the droplets are formed, the droplet preparation mechanism will collect the droplets. In certain embodiments, the consumables 3000 that have completed the collection of droplets will be transported to the forward transport position 41a of the installation chamber 11 through the forward transport window 12a under the transport of the forward transport mechanism 4a, so that the amplification analyzer 1000 completes subsequent amplification and analysis.

[0087] In some embodiments, the pre-cascade device 2000a is a pre-amplification analyzer. The splicing of the pre-amplification analyzer and the amplification analyzer 1000 can improve the throughput of the amplification analysis and improve the efficiency of the amplification analysis. Specifically, the pre-amplification analyzer can transport the consumables 3000 to be amplified to the forward transport position 41a of the installation cavity 11 through the forward transport mechanism 4a and the forward transport window 12a, so that the amplification analyzer 1000 can perform the amplification analysis of the consumables 3000 to be amplified together with the pre-amplification analyzer, increase the number of consumables that the detection system can complete the amplification analysis per unit time, and improve the amplification efficiency of the detection system. In some embodiments, the pre-amplification analyzer can also only complete the loading of the consumables 3000 to be amplified without performing the amplification analysis, and the current amplification analyzer 1000 completes the amplification analysis, which is also conducive to improving the loading efficiency of the consumables 3000.

[0088] In some embodiments, the amplification analyzer 1000 includes a consumables cache position 111c. When the amplification mechanism 3 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified from the forward transport position 41a to the consumables cache position 111c for caching. When the amplification mechanism 3 is in an idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified and analyzed to the amplification mechanism 3 for amplification.

[0089] In some embodiments, the installation cavity includes a consumable cache position 111c and a backward transfer position 41b. The processor 1001 of the amplification analyzer 1000 calls the third online mode stored in the memory 1005. In the third online mode, the processor controls the scheduling mechanism 2 to extract the consumable 3000 to be amplified from the consumable cache position 111c and transfer it to the backward transfer position 41b, or the processor controls the scheduling mechanism 2 to directly extract the consumable 3000 to be amplified from the forward transfer position and transfer it to the backward transfer position 41b.

[0090] Specifically, when the processor 1001 controls the scheduling mechanism 2 to transfer the consumables 3000, it will confirm the operating state of the amplification mechanism to determine whether the amplification mechanism is in an idle state or a non-idle state. In some embodiments, the processor 1001 can determine whether the number of consumables 3000 on the amplification mechanism 3 is less than the total number that can be accommodated. If so, it is determined that the amplification mechanism 3 is in an idle state, otherwise it is determined that the amplification mechanism 3 is in a non-idle state. In some embodiments, the processor 1001 may also only be in an empty state when the amplification mechanism 3 is in an empty state, that is, whether the number of consumables 3000 on the amplification mechanism 3 is zero. If so, it is determined that the amplification mechanism 3 is in an idle state, otherwise it is determined that the amplification mechanism 3 is in a non-idle state.

[0091] Therefore, by setting the consumable cache position 111c, more consumables can be loaded or produced through the front cascade device 2000a. When the amplification mechanism 3 is in a non-idle state, the forward transport mechanism 4a can still transport the consumables and cache them in the consumable cache position 111c, which is beneficial to improving the consumable loading efficiency.

[0092] In some embodiments, at least a portion of the forward transport mechanism 4a extends in the first direction in the installation cavity 11, and the amplification mechanism 3 and the signal analysis mechanism 5 are arranged side by side in the first direction. The extension direction of the forward transport mechanism 4a in the installation cavity 11 is consistent with the arrangement direction of the amplification mechanism 3 and the signal analysis mechanism 5, which can reduce the space occupied by the forward transport mechanism 4a in the first direction, reduce the length of the installation cavity 11 in the first direction, and also shorten the movement stroke of the scheduling mechanism 2 in the first direction, thereby improving the scheduling efficiency of the scheduling mechanism 2 for the consumables 3000. The arrangement of the amplification mechanism 3 and the signal analysis mechanism 5 along the extension direction of the forward transport mechanism 4a can reduce the distance of the consumables 3000 from the amplification mechanism 3 to the signal analysis mechanism 5, shorten the time of the consumables 3000 from the amplification mechanism 3 to the signal analysis mechanism 5, and improve the scheduling efficiency of the consumables 3000.

[0093] Please refer to Figure 7 In some embodiments, the transfer window 12 is a backward transfer window 12b, and the transfer mechanism 4 is a backward transfer mechanism 4b. The amplification analyzer 1000 is connected to the rear amplification analyzer 2000b through the backward transfer window 12b. One end of the backward transfer mechanism 4b is arranged or extended in the installation cavity 11, and the other end is arranged or extended in the rear amplification analyzer 2000b after passing through the backward transfer window 12b. The backward transfer mechanism 4b is used to transfer the consumables in the amplification analyzer 1000 to the rear amplification analyzer 2000b, so that the rear amplification analyzer can perform amplification analysis on the consumables 3000 to be amplified together with the current amplification analyzer 1000, thereby increasing the number of consumables that can be amplified and analyzed by the detection system per unit time, and improving the amplification efficiency of the detection system.

[0094] Correspondingly, a rearward transfer position 41b for transferring the consumables 3000 to the rearward amplification analyzer 2000b is provided in the installation cavity 11. The rearward transfer position 41b and the rearward transfer mechanism 4b overlap at least partially in the installation cavity 11, and the rearward transfer mechanism 4b can transfer the consumables on the rearward transfer position 41b to the rearward amplification analyzer 2000b. In some embodiments, a consumable loading position 111a for loading consumables is provided in the installation cavity 11.

[0095] In some embodiments, the processor 1001 of the amplification analyzer 1000 calls the second online mode stored in the memory 1005. In the second online mode, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified in the consumable loading position 111a, and transfers it to the backward transport position 41b, so as to transport it to the post-amplification analyzer through the backward transport mechanism 4b, so that the post-amplification analyzer performs amplification analysis.

[0096] Specifically, the backward transport mechanism 4b can be pre-set in the amplification analyzer 1000; it can also be pre-set in the post-amplification analyzer 2000b; it can also be an independent component independent of the amplification analyzer 1000 and the post-amplification analyzer 2000b, and it is only assembled and installed when the amplification analyzer 1000 and the post-amplification analyzer 2000b are spliced. The present application does not impose any restrictions on this.

[0097] Please refer to Figure 1 In some embodiments, the shell 1 also includes a loading window 13, and the consumables 3000 to be amplified are placed into the consumable loading position 111a through the loading window 13, and the backward transfer window 12b and the loading window 13 are arranged on adjacent sides of the shell 1, which is conducive to reducing the volume of the amplification analyzer 1000 and improving the user experience.

[0098] In some embodiments, the backward transport mechanism 4b is controlled by the processor of the amplification analyzer 1000. Specifically, the processor controls the operation of the backward transport mechanism 4b and obtains the state of the backward transport mechanism 4b. For example, under the control of the processor of the amplification analyzer 1000, the scheduling mechanism 2 first transfers the consumables to the backward transport position 41b, and then controls the backward transport mechanism 4b to transfer the consumables to the rear amplification analyzer 2000b through the backward transport window 12b. In some embodiments, the backward transport mechanism 4b is controlled by the processor of the rear amplification analyzer 2000b. Specifically, the processor controls the operation of the backward transport mechanism 4b and obtains the state of the backward transport mechanism 4b. For example, under the control of the processor of the amplification analyzer 1000, the scheduling mechanism 2 will transfer the consumables to the rear transfer position 41b. After that, the processor of the amplification analyzer 1000 will send the status information of the scheduling mechanism 2 to the processor of the rear amplification analyzer 2000b through the information interaction mechanism between the processor of the rear amplification analyzer 2000b directly or indirectly (through the host computers of both), and finally, the processor of the rear amplification analyzer 2000b will control the rear transfer mechanism 4b to transfer the consumables to the rear amplification analyzer 2000b through the rear transfer window 12b. In some embodiments, the rear transfer mechanism 4b is controlled by the processor of the host computer of the amplification analyzer 1000 and the rear amplification analyzer 2000b. Specifically, the processor of the host computer directly or indirectly controls the work of the rear transfer mechanism 4b, obtains the state of the rear transfer mechanism 4b, and directly or indirectly controls the rear transfer mechanism 4b to transfer the consumables 3000 on the rear transfer position 41b according to the state.

[0099] In some embodiments, the installation cavity 11 further includes a consumables cache position 111c and / or a forward transfer position; the processor 1001 of the amplification analyzer 1000 calls the third online mode stored in the memory 1005. In the third online mode, the processor controls the scheduling mechanism 2 to extract the consumables to be amplified from one of the forward transfer position 41a and the consumables cache position 111c to the amplification mechanism 3 for amplification, and then transfers it to the signal analysis mechanism 5 to determine and output the corresponding analysis results. Alternatively, the processor controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified from the consumables cache position 111c to the backward transfer position 41b.

[0100] In some embodiments, please refer to Figure 8The transfer window 12 includes a forward transfer window 12a and a backward transfer window 12b. The amplification analyzer 1000 is connected to the front cascade device 2000a through the forward transfer window 12a. One end of the forward transfer mechanism 4a is set or extended in the installation cavity 11, and the other end passes through the forward transfer window 12a and is set or extended in the front cascade device 2000a. The amplification analyzer 1000 is connected to the backward amplification analyzer 2000b through the backward transfer window 12b. One end of the backward transfer mechanism 4b is set or extended in the installation cavity 11, and the other end passes through the backward transfer window 12b and is set or extended in the backward amplification analyzer 2000b.

[0101] Correspondingly, the installation cavity 11 is provided with a forward transfer position 41a and a backward transfer position 41b. At this time, the processor of the amplification analyzer 1000 calls the third online mode stored in the memory. In the third online mode, when the amplification analyzer 1000 meets the preset transportation condition, the processor controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified in the forward transfer position 41a, and directly transfers it to the backward transfer position 41b, so as to transport it to the rear amplification analyzer 2000b through the backward transfer mechanism 4b. Through the direct transportation of the amplification analyzer 1000, the rear amplification analyzer 2000b can directly perform amplification analysis on the consumables 3000 that have been completed by the pre-cascade device and have been prepared by droplets, which can meet the needs of specific scenarios, especially the situation where the droplet preparation efficiency is higher than the amplification analysis efficiency. Specifically, the preset transportation condition can be that the amplification mechanism 3 is in a non-idle state, or that the processor 1001 receives a user instruction, or that other preset scheduling conditions are met, and this application does not limit this.

[0102] In some embodiments, when the amplification mechanism 3 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified and analyzed from the forward transfer position 41a to the consumables cache position 111c for caching; when the amplification mechanism 3 is in an idle state, the scheduling mechanism 2 is controlled to extract the consumables 3000 to be amplified and analyzed to the amplification mechanism 3 for amplification. In some embodiments, when the amplification mechanism 3 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified and analyzed from the forward transfer position 41a to the consumables cache position 111c for caching, and then the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be amplified and analyzed on the consumables cache position 111c to the backward transfer position 41b, so that the consumables to be amplified and analyzed are transported to the post-amplification analyzer 2000b through the backward transfer position 41b.

[0103] In some embodiments, when the signal analysis mechanism 5 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be analyzed for signal from the amplification mechanism 3 to the consumables cache position 111c for caching; when the signal analysis mechanism 5 is in an idle state, the scheduling mechanism 2 is controlled to extract the consumables 3000 to be analyzed for signal to the signal analysis mechanism 5 for analysis. In some embodiments, when the signal analysis mechanism 5 is in a non-idle state, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be analyzed for signal from the amplification mechanism 3 to the consumables cache position 111c for caching; thereafter, the processor 1001 controls the scheduling mechanism 2 to extract the consumables 3000 to be analyzed for signal on the consumables cache position 111c to the backward transfer position 41b, thereby transferring the consumables to be analyzed for signal to the post-amplification analyzer 2000b through the backward transfer position 41b for signal analysis.

[0104] In some embodiments, the processor 1001 can determine whether to perform the cache action by judging whether the number of consumables 3000 on the backward transfer position 41b is greater than the total number that can be accommodated or whether it is cleared. If so, the processor 1001 will control the scheduling mechanism 2 to extract the consumables 3000 to be analyzed for amplification from the forward transfer position 41a to the consumable cache position 111c for cache, and then, when the backward transfer position 41b is idle, the scheduling mechanism 2 will be controlled to extract the consumables 3000 to be analyzed for amplification to the backward transfer position 41b.

[0105] It should be noted that the forward transfer mechanism 4a can be fixed in the installation cavity 11 of the amplification analyzer 1000, and extend out of the installation cavity 11 and into the front cascade device 2000a through the forward transfer window 12a, and be arranged or extended in the front cascade device; of course, the forward transfer mechanism 4a can also be fixed in the front cascade device 2000a, and extend into the installation cavity 11 through the forward transfer window 12a, and be arranged or extended in the amplification analyzer 1000. Similarly, the backward transport mechanism 4b can be fixedly disposed in the installation cavity 11 of the amplification analyzer 1000, and extend out of the installation cavity 11 through the backward transport window 12b and extend into the rear amplification analyzer 2000b, and be disposed or extended in the rear amplification analyzer 2000b; the backward transport mechanism 4b can also be fixed in the rear amplification analyzer 2000b, and extend into the installation cavity 11 through the backward transport window 12b, and be disposed or extended in the amplification analyzer 1000, and the present application does not impose any restrictions on this.

[0106] Specifically, taking the case where a part of the backward transport mechanism 4b is fixed in the rear-end amplification analyzer 2000b as an example, when the amplification analyzer 1000 and the rear-end amplification analyzer 2000b are spliced, the other part of the backward transport mechanism 4b can be fixed in the installation cavity 11 through the backward transport window 12b, or it can only extend in the space of the installation cavity 11. In one example, the two parts of the backward transport mechanism 4b are detachable, and when the amplification analyzer 1000 and the rear-end amplification analyzer 2000b are spliced, the part of the backward transport mechanism 4b extending into the amplification analyzer 1000 is installed, which does not affect the use of both the amplification analyzer 1000 and the rear-end amplification analyzer 2000b in the stand-alone mode. Similarly, when the amplification analyzer 1000 and the front-end cascade device 2000a are connected, the forward transport mechanism 4a can refer to the design of the backward transport mechanism 4b.

[0107] It should be noted that the consumables buffer position 111c, the waste placement position 111b, and the consumables loading position 111a referred to above may be independent spaces spaced apart in the installation cavity 11, or may at least partially overlap in the space in the installation cavity. In one possible implementation of the present application, at least two of the consumables buffer position 111c, the waste placement position 111b, and the consumables loading position 111a are reused positions, that is, at least two of the consumables buffer position 111c, the waste placement position 111b, and the consumables loading position 111a overlap in space in the installation cavity 11. For example, the reuse of the consumables loading position 111a and the consumables buffer position 111c can improve the space utilization rate of the installation cavity 11.

[0108] In some embodiments, the amplification mechanism 3 and the signal analysis mechanism 5 are arranged side by side along the first direction, the consumable sample loading position 111a and the amplification mechanism 3 are arranged side by side along the second direction, and a first loading space extending along the first direction is provided in the installation cavity 11, and the first loading space is used for the installation of the backward transport mechanism 4b, wherein the first direction and the second direction are perpendicular to each other. By providing the first loading space extending along the first direction, the space occupied by the backward transport mechanism 4b in the installation cavity 11 in the first direction can be effectively reduced, the length of the installation cavity 11 in the first direction can be shortened, and the dispatching efficiency of the dispatching mechanism 2 can be improved.

[0109] In some embodiments, the forward transfer mechanism 4a extends in the installation cavity 11 along the first direction, and the extension portion is at least partially located between the consumables cache position 111c and the amplification mechanism 3; the consumables cache position 111c and the amplification mechanism 3 are arranged side by side along the second direction, wherein the first direction and the second direction are perpendicular to each other. By arranging the forward transfer mechanism 4a at the consumables cache position 111c and the amplification mechanism 3, the distance of the scheduling mechanism 2 from the forward transfer position 41a to the consumables cache position 111c, or from the forward transfer position 41a to the amplification mechanism 3 to schedule the consumables 3000 can be reduced, and the scheduling time of the scheduling mechanism 2 between the forward transfer position 41a and the consumables cache position 111c, or the forward transfer position 41a or the amplification mechanism 3 can be reduced, thereby improving the scheduling efficiency of the scheduling mechanism 2. Similarly, the relative arrangement of the consumable cache position 111c and the amplification mechanism 3 in the second direction can also reduce the distance of the consumable cache position 111c to dispatch the consumable 3000 to the amplification mechanism 3, reduce the scheduling time of the scheduling mechanism 2 between the consumable cache position 111c and the amplification mechanism 3, and improve the scheduling efficiency of the scheduling mechanism 2.

[0110] In some embodiments, in order to improve the reliability of the detection system, before starting / activating the transport mechanism 4, the processor 1001 will also detect the online status of the amplification analyzer 1000 and the front cascade device 2000a, or the amplification analyzer 1000 and the rear amplification analyzer 2000b, or the amplification analyzer 1000 and the front cascade device 2000a and the rear amplification analyzer 2000b. The online status includes: whether the amplification analyzer 1000, the front cascade device 2000a, and the rear amplification analyzer 2000b are mechanically spliced ​​with each other; whether the amplification analyzer 1000, the front cascade device 2000a, and the rear amplification analyzer 2000b have established an effective connection in communication; whether the forward transport mechanism 4a and the rear transport mechanism 4b can travel smoothly between the amplification analyzer 1000, the front cascade device 2000a, and the rear amplification analyzer 2000b, etc. When the above connection states are all kept normal, the transfer mechanism 4 is controlled to exchange information, otherwise, the transfer mechanism is prohibited from working, and the user is reminded that the connection between the amplification analyzer 1000 and the front cascade device 2000a, or the amplification analyzer 1000 and the rear amplification analyzer 2000b is abnormal. In this way, the amplification analyzer 1000 is prevented from idling or being blocked due to the amplification analyzer 1000 not being connected to the front cascade device 2000a or the connection being abnormal, thereby improving the reliability of the detection system operation.

[0111] In some embodiments, please refer to Fig. 9, the working modes stored in the memory include stand-alone mode. In stand-alone mode, the user will first place the consumable 3000 to be amplified and analyzed on the consumable loading position 111a. The processor 1001 will set the consumable loading position 111a as the first placement position and the waste placement position 111b as the second placement position. Afterwards, the processor 1001 controls the scheduling mechanism 2 to extract the consumable 3000 to be amplified from the consumable loading position 111a, and transfer it to the amplification mechanism 3 for amplification, and then transfer it to the signal analysis mechanism 5 for signal analysis to determine and output the corresponding analysis results, and finally transfer the consumable 3000 that has completed amplification to the waste placement position 111b, thereby completing the amplification and analysis of the consumable 3000.

[0112] The transfer mechanism 4 can be a manipulator, a conveyor belt, or a mechanical mobile platform, and this application does not limit this. Figure 2 and Fig.10 In some embodiments, the transfer mechanism 4 includes a guide rail 42, a mobile platform 43, and a drive assembly 44. The guide rail 42 is disposed in the installation cavity 11, and the mobile platform 43 is slidably disposed on the guide rail 42. The drive assembly 44 is used to drive the mobile platform 43 to slide along the guide rail 42. The drive assembly can be a motor or a cylinder, and the present application does not limit this.

[0113] In actual application, when the amplification analyzer 1000 and the cascade device 2000 are spliced, the guide rail 42 is provided between the installation cavity 11 of the amplification analyzer 1000 and the cascade device 2000 through the transfer window. The mobile platform 43 can slide along the guide rail 42 under the drive of the driving component 44, so as to transfer the consumables in the installation cavity 11 to the cascade device 2000, or transfer the consumables in the cascade device 2000 to the installation cavity 11. In some embodiments, the guide rail 42 can be provided only in one of the amplification analyzer 1000 and the cascade device 2000, but the mobile platform 43 is in a part of the guide rail 42 (for example, the farthest end), and the mobile platform 43 extends inside the other one of the amplification analyzer 1000 and the cascade device 2000.

[0114] In some application scenarios, due to the layout of the device itself, the scheduling mechanism in the amplification analyzer 1000 and the scheduling mechanism in the cascade device 2000 may schedule consumables in different directions. Figure 3 For example, Figure 3 The scheduling mechanism 2 of the amplification analyzer 1000 schedules the consumables along the second direction Y, while the scheduling mechanism in the cascade device 2000 schedules the consumables along the first direction Y. In order to facilitate the scheduling mechanisms of different devices to schedule the consumables on the mobile platform 43, please refer to Fig.11In one embodiment, the mobile platform 43 includes a loading seat 431 and a rotating seat 432. The loading seat is slidably disposed on the guide rail 42, and the rotating seat is rotatably disposed on the loading seat. The rotating seat has a first position extending along a first direction and a second position extending along a second direction relative to the device seat. When the mobile platform 43 is located in the amplification analyzer 1000, the rotating seat 432 is located in the second position, and when the mobile platform 43 is located in the cascade device 2000, the rotating seat 432 is located in the first position. In this way, the scheduling direction between the various devices is coordinated to realize the flexible turning of the consumables, and the scheduling fluency of the scheduling mechanism for the consumables is improved.

[0115] Specifically, the transfer mechanism includes a reversing component, which is used to drive the rotating seat to switch between the first position and the second position. The reversing component can be a motor, a rotary cylinder, or other rotary drive components, which is not limited in this application. Fig.12 and Fig.13 In one embodiment, the reversing assembly 433 includes a push member 4331 and an elastic member 4332. The push member 4331 is disposed on the motion path of the mobile platform 43. The push member 4331 is configured to contact the rotating seat 432 when the rotating seat 432 reaches a set position (for example, reaches the farthest position of the guide rail), and push the rotating seat 432 to rotate from the second position to the first position. The elastic member 4332 is disposed between the rotating seat 432 and the loading seat 431. The elastic member 4332 is configured to drive the rotating seat 432 to reset from the first position to the second position when the push member 4331 is separated from the rotating seat 432, thereby realizing unpowered reversing.

[0116] In specific applications, such as Fig.13 As shown, in the initial state of the amplification analyzer 1000, the rotating seat 432 is in the second position. Fig.14 When the mobile platform 43 moves along the guide rail 42, the rotating seat 432 will contact the push member 4331 and rotate from the second position to the first position under the push of the push member 4331. Fig.15 As shown, when the mobile platform 43 reaches the preset position, the rotating seat 432 contacts the limiting member 4333 and is fixed at the first position under the limiting of the limiting member 4333 and the pushing member 4331. When the mobile platform 43 leaves the amplification analyzer 1000, the rotating seat 432 separates from the pushing member 4331, and then the rotating seat 432 returns from the first position to the second position under the action of the elastic member 4332, thereby achieving reversal.

[0117] It should be noted that in some embodiments, the preset position can be set before the forward transfer position or the backward transfer position, that is, the rotating seat 432 has been rotated to the first position before reaching the forward transfer position 41a or the backward transfer position 41b. In some embodiments, the preset position can also be set just at the forward transfer position or the backward transfer position, that is, when the rotating seat 432 moves to the forward transfer position 41a or the backward transfer position 41b, the rotating seat 432 is fixed to the first position by the push member 4331.

[0118] Please refer to Figure 3 The amplification analyzer 1000 also includes a liquid path mechanism 6, which is used to provide an injection channel and a sample oil carrier channel. The injection channel is responsible for introducing the sample to be analyzed into the amplification reaction system to ensure that the sample can smoothly enter the amplification reactor for polymerase chain reaction. The sample oil carrier channel is used to form a suitable environment to prevent the sample from evaporating or contaminating during the reaction process and to ensure the stability and accuracy of the reaction.

[0119] In some embodiments, the fluid path mechanism 6 and the signal analysis mechanism 5 are arranged side by side along the second direction, wherein the first direction and the second direction are perpendicular to each other. In this way, the space occupied by the fluid path mechanism 6 in the first direction of the installation chamber 11 is reduced, the distance between the forward transfer position 41a and the backward transfer position 41b is reduced, the movement time of the dispatching mechanism 2 between the forward transfer position 41a and the backward transfer position 41b is reduced, and the dispatching efficiency of the dispatching mechanism 2 is improved.

[0120] In some embodiments, a first loading space extending along the first direction is provided between the signal analysis mechanism 5 and the liquid circuit mechanism 6; the first loading space is used for at least partial installation of the transport mechanism (i.e., the backward transport mechanism 4b) in the rear amplification analyzer 2000b. By arranging the backward transport mechanism 4b along the first direction between the signal analysis mechanism 5 and the liquid circuit mechanism 6, the space occupied by the backward transport mechanism 4b in the installation chamber 11 in the first direction is reduced, the distance between the forward transport position 41a and the backward transport position 41b is reduced, the movement time of the dispatch mechanism 2 between the forward transport position 41a and the backward transport position 41b is reduced, and the dispatch efficiency of the dispatch mechanism 2 is improved.

[0121] Please refer to Fig.16 The present application also proposes a control method for an amplification analyzer, which is stored in a memory and executed by a processor to control the amplification analyzer to work. The control method includes:

[0122] S101, receiving user instructions.

[0123] S102, determining a working mode corresponding to the instruction according to the user instruction, and determining a first placement position for consumables to be amplified according to the corresponding working mode; and / or determining a second placement position for consumables that have completed amplification.

[0124] S103, the scheduling mechanism extracts the consumables to be amplified in the first placement position, transfers them to the amplification mechanism for amplification, and then transfers them to the signal analysis mechanism for signal analysis to determine and output the corresponding analysis results, and finally transfers the consumables that have completed amplification to the second placement position.

[0125] The instructions that the user can send to the processor include: at least two of the stand-alone mode instructions, the first online mode instructions, the second online mode instructions, and the third online mode instructions. The working mode includes: at least two of the stand-alone mode, the first online mode, the second online mode, and the third online mode. The first placement position includes at least one of the consumable loading position, the forward transfer position, and the consumable buffer position; the second placement position includes at least one of the waste placement position, the backward transfer position, or the consumable buffer position.

[0126] Please refer to Fig.17 , determining a working mode corresponding to the instruction according to the user instruction, and determining a first placement position of the consumable to be amplified according to the corresponding working mode; and / or determining a second placement position of the consumable that has completed amplification includes:

[0127] S1021. When receiving a stand-alone mode instruction, switch the working mode to the stand-alone mode, and set the consumable loading position to the first placement position, and set the waste placement position to the second placement position; and / or

[0128] S1022, when receiving the first online mode instruction, switching the working mode to the first online mode, and setting the forward transfer position as the first placement position, and setting the waste placement position or the consumables buffer position as the second placement position; and / or

[0129] S1023, when receiving the second online mode instruction, switching the working mode to the second online mode, and setting the consumable loading position or the consumable buffer position as the first placement position, and setting the backward transfer position as the second placement position; and / or

[0130] S1024. When receiving the third online mode instruction, switch the working mode to the third online mode, set the forward transfer position as the first placement position, set the backward transfer position as the second placement position, and the processor controls the scheduling mechanism to extract the consumables to be amplified from the first placement position to the second placement position.

[0131] Please refer to Fig.18Specifically, in the third online mode, the processor controls the scheduling mechanism to extract the consumables to be amplified from the first placement position to the second placement position, which may include the following:

[0132] S10241. When the amplification analyzer meets the preset transfer conditions, the processor controls the scheduling mechanism to extract the consumables to be amplified in the forward transfer position and directly transfer them to the backward transfer position.

[0133] S10242. When the amplification mechanism is in a non-idle state, the processor controls the scheduling mechanism to extract the consumables to be amplified from the forward transfer position and cache them in the consumable cache position; when the amplification analyzer meets the preset transfer conditions, the processor controls the scheduling mechanism to extract the consumables to be amplified from the consumable cache position and directly transfer them to the backward transfer position.

[0134] S10243. When the signal analysis mechanism is in a non-idle state, the processor controls the scheduling mechanism to extract the consumables to be analyzed for signal from the amplification mechanism to the consumable cache for caching; when the signal analysis mechanism is in an idle state, the processor controls the scheduling mechanism to extract the consumables to be analyzed for signal to the signal analysis mechanism for analysis.

[0135] Therefore, the amplification analyzer of the embodiment of the present application can select different working modes according to different scene requirements, and determine the corresponding first placement position and second placement position according to different working modes. The processor can control the scheduling mechanism to extract the consumables at the first placement position and place them on the second placement position. By selecting different first placement positions and second placement positions, the amplification analyzer can not only complete the single-machine analysis of consumables, but also can be cascaded with other devices with the cooperation of the transfer window and the transfer mechanism, so as to expand the use scenarios of the amplification analyzer and improve the flexibility of the use of the amplification analyzer.

[0136] It should be noted that in this application Figure 6-Figure 9 The dispatching mechanism is for illustration only and does not represent the specific location of the dispatching mechanism of this equipment.

[0137] It should be noted that in the present application, the processor can be a central processing unit (CPU), or other general-purpose processors, digital signal processors (DSP), application-specific integrated circuits (ASIC), field-programmable gate arrays (FPGA), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc., and the present application does not impose any restrictions on this.

[0138] The memory stores an operating system, a network communication module, a user interface module, and an amplification analyzer control program. The memory may be a volatile memory or a nonvolatile memory, or may include both volatile and nonvolatile memories. Among them, the nonvolatile memory may be a read-only memory (ROM), a programmable read-only memory (PROM), an erasable programmable read-only memory (EPROM), an electrically erasable programmable read-only memory (EEPROM), or a flash memory. The volatile memory may be a random access memory (RAM), which is used as an external cache. By way of example but not limitation, many forms of RAM are available, such as static random access memory (SRAM), dynamic random access memory (DRAM), synchronous dynamic random access memory (SDRAM), double data rate synchronous dynamic random access memory (DDR SDRAM), enhanced synchronous dynamic random access memory (ESDRAM), synchronous link DRAM (SLDRAM) and direct RAM bus random access memory (DR RAM).

[0139] In the description of the embodiments of the present application, it should be noted that the orientation or positional relationship of terms such as "center", "up", "down", "left", "right", "vertical", "horizontal", "inside" and "outside" are based on the orientation or positional relationship described in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application.

[0140] What is disclosed above is only a preferred embodiment of the present application, and it certainly cannot be used to limit the scope of rights of the present application. Ordinary technicians in this field can understand that all or part of the processes of implementing the above embodiment and equivalent changes made according to the claims of the present application are still within the scope covered by the present application.

Claims

1. An amplification analyzer, characterized in that: It comprises a processor, a memory connected to the processor, a shell, a scheduling mechanism, an amplification mechanism and a signal analysis mechanism; the shell comprises an installation cavity and at least one transfer window, the installation cavity has a consumable loading position for loading consumables to be amplified and analyzed and a waste placement position for placing consumables that have completed amplification and analysis; the transfer window is used to cooperate with the transfer mechanism to transfer the consumables to be amplified and analyzed into the installation cavity and / or transfer the consumables that have completed amplification and analysis out of the installation cavity, the transfer mechanism has a forward transfer position for transferring the consumables to be amplified and analyzed into the installation cavity and / or a backward transfer position for transferring the consumables that have completed amplification and analysis out of the installation cavity; the amplification mechanism, the analysis mechanism and the scheduling mechanism are arranged in the installation cavity, and are all controlled by the processor; The memory is used to store at least two working modes for the processor to call respectively; The processor is configured to determine, according to the user's instruction, a working mode corresponding to the instruction, determine, according to the corresponding working mode, a first placement position for consumables to be amplified and analyzed, and / or determine a second placement position for consumables that have completed amplification and analysis, and control the scheduling mechanism to extract the consumables to be amplified and analyzed from the first placement position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the second placement position; Among them, the first placement position includes the consumables loading position or the forward transfer position; the second placement position includes the waste placement position or the backward transfer position.

2. The amplification analyzer according to claim 1, characterized in that: The working mode includes a stand-alone mode; In the stand-alone mode, the first placement position is a consumable loading position, and the second placement position is a waste placement position; The processor is configured to control the scheduling mechanism to extract the consumables to be amplified and analyzed from the consumable loading position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the waste placement position.

3. The amplification analyzer according to claim 1, characterized in that: The working mode also includes a first online mode; a forward transport mechanism is arranged in the installation cavity, the forward transport mechanism is used to connect with the front cascade device, and the forward transport mechanism is controlled by the processor, or by the processor of the front cascade device, or by the processor of the amplification analyzer and the host computer of the front cascade device; In the first online mode, the first placement position is a forward transfer position, and the second placement position is a waste placement position; The processor is configured to control the scheduling mechanism to extract the consumables to be amplified and analyzed in the forward transfer position, transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism to determine and output the corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the waste placement position.

4. The amplification analyzer according to claim 3, characterized in that: The amplification analyzer also includes a consumables cache location, which is used to store the consumables to be amplified and analyzed that are transported by the forward transport mechanism; The processor is configured to control the scheduling mechanism to transfer the consumables to be amplified and analyzed on the forward transfer position to the consumables cache position according to preset scheduling rules or user instructions when the amplification and analysis mechanism is in a non-idle state; and to control the scheduling mechanism to transfer the consumables to be amplified and analyzed on the consumables cache position to the amplification mechanism for amplification when the amplification and analysis mechanism is in an idle state according to preset scheduling rules or user instructions, and then transfer them to the signal analysis mechanism to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the waste placement position.

5. The amplification analyzer according to claim 1, characterized in that: The working mode also includes a second online mode; a backward transport mechanism is arranged in the installation cavity, the backward transport mechanism is used to connect with the post-amplification analyzer, and the transport mechanism is controlled by the processor, or by the post-amplification analyzer, or by the processor of the amplification analyzer and the host computer of the post-amplification analyzer; In the second online mode, the first placement position is a consumable loading position, and the second placement position is a backward transfer position; The processor is configured to control the scheduling mechanism to extract the consumables to be amplified and analyzed from the consumable loading position, and transfer them to the backward transfer position, so as to transport them to the post-amplification analyzer through the backward transfer mechanism.

6. The amplification analyzer according to claim 1, characterized in that: The working mode also includes a third online mode, the transfer window includes a forward transfer window and a backward transfer window; a transfer mechanism is arranged in the installation cavity, the transfer mechanism includes a forward transfer mechanism and a backward transfer mechanism, the forward transfer mechanism extends into the installation cavity through the forward transfer window, and the forward transfer position is arranged in the forward transfer mechanism; the backward transfer mechanism extends out of the installation cavity through the backward transfer window; the backward transfer position is arranged in the backward transfer mechanism; the forward transfer mechanism is used to connect with the front cascade device, and the forward transfer mechanism is controlled by the processor, or by the processor of the front cascade device, or by the processor of the upper computer of the amplification analyzer and the front cascade device; the backward transfer mechanism is used to connect with the rear amplification analyzer, and the transfer mechanism is controlled by the processor, or by the processor of the rear amplification analyzer, or by the processor of the amplification analyzer and the upper computer of the rear amplification analyzer; In the third online mode, the first placement position is a forward transfer position, and the second placement position is a backward transfer position; The processor is configured to control the dispatch mechanism to extract the consumables to be amplified and analyzed in the forward transport position and transfer them to the backward transport position so as to be transported to the post-amplification analyzer through the backward transport mechanism; and / or The processor is configured to control the scheduling mechanism to extract the consumables to be amplified and analyzed in the forward transfer position and transfer them to the amplification mechanism for amplification, and then transfer them to the signal analysis mechanism for signal analysis to determine and output corresponding analysis results, and finally transfer the consumables that have completed amplification and analysis to the waste placement position.

7. The amplification analyzer according to claim 5 or 6, characterized in that: The processor is configured to, when the amplification mechanism is in a non-idle state, control the scheduling mechanism to extract the consumables to be amplified and transfer them to the backward transfer position, either according to preset scheduling conditions or according to user instructions.

8. The amplification analyzer according to claim 6, characterized in that: The amplification analyzer also includes a consumables cache location, which is used to store the consumables to be amplified and analyzed that are transported by the forward transport mechanism; The processor is configured to control the scheduling mechanism to transfer the consumables to be amplified and analyzed on the forward transfer position to the consumable cache position, and when the backward transfer position is idle, control the scheduling mechanism to transfer the consumables to be amplified and analyzed on the consumable cache position to the backward transfer position according to preset scheduling rules.

9. The amplification analyzer according to claim 4 or 8, characterized in that: The consumables buffer position and the consumables loading position structure are multiplexed.

10. A detection system, characterized in that: It comprises a cascade device and an amplification analyzer as described in any one of claims 1 to 9; at least one of the cascade device and the amplification analyzer is provided with the transfer mechanism; the transfer mechanism is connected between the amplification analyzer and the cascade device and passes through the transfer window; the transfer mechanism is used to transfer consumables in the amplification analyzer and the cascade device.

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