Microbial flora fermentation process and preparation method

Abstract

The invention relates to the technical field of microbial flora preparation, in particular to a microbial flora fermentation process and a preparation method. In the microbial flora fermentation process, an equipment body is adopted to perform fermentation preparation on microbial flora, fermentation equipment comprises a tank body, an equipment inner cavity is internally provided with a cavity adjusting mechanism, the cavity adjusting mechanism comprises a cavity bottom plate, and the equipment inner cavity is divided into a driving cavity and a fermentation cavity by the cavity bottom plate; an upper area ring body and a lower area ring body are fixedly mounted on the inner wall of the fermentation cavity, a driving mechanism is mounted in the driving cavity, the other end of the driving mechanism is connected with the bottom of the cavity bottom plate, the driving mechanism can drive the cavity bottom plate to slide in the fermentation cavity, and when the microbial flora is subjected to initial fermentation, the cavity bottom plate slides at the upper area ring body; when the microbial flora is in the last fermentation stage, the cavity bottom plate slides at the lower area ring body, and meanwhile, the sliding of the cavity bottom plate can also perform mixing treatment on the microbial flora in the microbial fermentation process.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial flora preparation, and in particular to a microbial flora fermentation process and a preparation method. Background Art

[0002] Microbial fermentation refers to the process of using microorganisms to convert raw materials into products needed by humans through specific metabolic pathways under suitable conditions. The production level of microbial fermentation mainly depends on the genetic characteristics of the strain itself and the culture conditions.

[0003] During fermentation, the microorganisms need to be placed in a fermentation device, such as a microbial fermentation device with Chinese patent publication number CN206940857U, which consists of an outer box and a fermentation container placed inside the outer box, a liquid inlet device is provided at the upper part of one side of the fermentation container, and a liquid outlet device is provided at the lower part of the other side, and the inlet and outlet of the liquid inlet device are extended to the outside of the outer box, a thermometer, a stirring device, a heat source and an artificial light source are provided inside the fermentation container, and each surface of the outer box is made of a shading plate, wherein the shading plate on the top surface can be removed;

[0004] During the microbial fermentation process, microorganisms have different growth characteristics and space requirements at different stages. In the early stage of fermentation, microorganisms have just entered the new environment and are in the adaptation period and logarithmic growth period. The number is relatively small and the demand for space is not prominent. As the fermentation progresses, the microorganisms continue to multiply and the number increases significantly. However, the internal space of the fermentation tank is fixed. As the fermentation process proceeds, metabolic waste will be difficult to diffuse out quickly due to crowded space. Excessive accumulation of metabolic waste in local areas will change the microenvironment around the microorganisms, inhibit the activity of enzymes in the microorganisms, and then affect the generation of fermentation products, and ultimately reduce the effect of the entire fermentation.

[0005] In view of this, a microbial flora fermentation process and a preparation method are urgently needed to solve the above problems. Summary of the invention

[0006] The object of the present invention is to provide a microbial flora fermentation process and a preparation method to solve the problems raised in the above background technology.

[0007] To achieve the above object, the present invention provides a microbial flora fermentation process, which comprises the following steps:

[0008] S1.1, Fermentation preparation: Select microbial strains and raw materials for fermentation, prepare microorganisms, including carbon source, nitrogen source, inorganic salts, and then sterilize;

[0009] S1.2, fermentation: connecting the prepared microorganisms and fermentation raw materials to the fermentation equipment for fermentation;

[0010] S1.3, separation and purification: after the fermentation is completed, the fermentation products are separated and purified from the microbial cells and unconsumed nutrients;

[0011] S1.4, Concentration and drying: Concentrate and dry the separated and purified product;

[0012] The fermentation equipment comprises a tank body, a support is fixedly installed at the bottom of the tank body, a top cover is sealedly installed at the top of the tank body, a delivery pipe is installed inside the top cover, the interior of the tank body forms an equipment cavity, a middle cavity is opened inside the inner wall of the equipment cavity, and a heating plate is installed inside the middle cavity;

[0013] A cavity adjustment mechanism is arranged inside the inner cavity of the device, and the cavity adjustment mechanism includes a cavity bottom plate, and the cavity bottom plate divides the inner cavity of the device into a driving cavity and a fermentation cavity, and an upper region ring body and a lower region ring body are fixedly installed on the inner wall of the fermentation cavity, and a driving mechanism is installed inside the driving cavity, and the other end of the driving mechanism is connected to the bottom of the cavity bottom plate;

[0014] The driving mechanism can drive the cavity bottom plate to slide inside the fermentation cavity. When the microbial flora is in the initial stage of fermentation, the cavity bottom plate slides on the upper area ring body. When the microbial flora is in the final stage of fermentation, the cavity bottom plate slides on the lower area ring body. At the same time, the sliding of the cavity bottom plate can also mix the microbial flora during the fermentation process.

[0015] As a further improvement of the present technical solution, the cavity bottom plate includes a fixed ring and a movable plate. The fixed ring is fixedly installed inside the inner cavity of the equipment, and the movable plate slides inside the fixed ring. The outer wall of the movable plate fits with the inner wall of the fixed ring, and the top of the movable plate is flush with the top of the fixed ring.

[0016] As a further improvement of the present technical solution, the driving mechanism includes a cylinder body fixedly connected to the bottom of the inner cavity of the driving chamber, a piston rod is slidably provided inside the cylinder body, one end of the piston rod away from the cylinder body is fixedly connected to the bottom of the movable plate, the outer bottom of the movable plate is connected to an auxiliary part, and the auxiliary part is sleeved on the outside of the cylinder body.

[0017] As a further improvement of the present technical solution, the auxiliary part includes a fixed plate body, the bottom of which is fixedly installed on the bottom of the inner cavity of the driving cavity, an inner groove is opened inside the fixed plate body, a ring plate is slidably provided inside the inner groove, and the top of the ring plate is fixedly connected to the bottom of the movable plate.

[0018] As a further improvement of the technical solution, a sealing groove is provided on the inner end surface of the fixing ring, and a sealing sleeve is fixedly connected to the inner seal of the sealing groove. The other end of the sealing sleeve is fixedly installed on the bottom of the movable plate, and the sealing sleeve is sleeved on the outside of the auxiliary component.

[0019] As a further improvement of the technical solution, an extension page plate is fixedly installed on the top of the movable plate, and a plurality of flow holes are formed on the outer surface of the extension page plate.

[0020] As a further improvement of the technical solution, the extension page is made of elastic material.

[0021] The present invention also provides a preparation method, comprising the following steps:

[0022] S2.1. Selection of strains: Select the original strains;

[0023] S2.2, slant culture: inoculate the bacteria onto the slant culture medium to activate the bacteria;

[0024] S2.3, seed solution preparation: pick the activated strains and inoculate them into the seed culture medium;

[0025] S2.4, fermentation: ferment the microbial flora using the above-mentioned microbial flora fermentation process.

[0026] Compared with the prior art, the present invention has the following beneficial effects:

[0027] In the microbial flora fermentation process and preparation method, during the microbial fermentation process, when the microbial flora and the fermentation liquid are put into the fermentation chamber, the driving mechanism drives the movable plate to move to the vicinity of the upper region ring body in the fermentation chamber. At this time, the internal space of the fermentation chamber is relatively small, which is convenient for centralized ventilation, providing sufficient initial oxygen for the microorganisms, and helping the microorganisms to quickly adapt to the new environment and efficiently and centrally utilize nutrients. As the fermentation process progresses, the driving mechanism will play a role again, driving the movable plate to adjust its position in the fermentation chamber. When the fermentation is close to the end, the movable plate will be moved to the vicinity of the lower region ring body in the fermentation chamber, so that the space inside the fermentation chamber is increased compared with the initial stage of fermentation, and the air can be more evenly distributed in the fermentation liquid, thereby ensuring that oxygen is fully dissolved in the fermentation liquid to meet the continuous demand for oxygen of the microorganisms in the later stage of fermentation. At the same time, the waste gas such as carbon dioxide generated by the microorganisms during the metabolism process also has more discharge space, effectively avoiding the accumulation of these waste gases in the fermentation liquid, thereby preventing the adverse effects of waste gas accumulation on the metabolic activities of the microorganisms, and ensuring that the entire fermentation process can be completed smoothly and efficiently;

[0028] During the fermentation process, the driving mechanism will drive the movable plate to make it continuously and frequently perform small vertical movements near the upper and lower area rings. This movement mode can effectively promote the mixing between the microbial flora and the fermentation liquid, allowing the two to fully contact, ensuring the uniformity and efficiency of the fermentation process. Compared with traditional mechanical stirring methods, this mixing structure avoids the strong shear force caused by the high-speed rotation of traditional stirring blades, reduces the possibility of physical damage to the microbial flora, and provides a relatively mild and stable fermentation environment for microorganisms. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0030] Figure 2 It is a schematic diagram of the cutaway structure of the tank body of the present invention;

[0031] Figure 3 It is a cutaway front view of the tank body of the present invention;

[0032] Figure 4 It is a schematic diagram of the structure of the fixed ring and the movable plate integrated into one according to the present invention;

[0033] Figure 5 For the present invention Figure 4 A schematic diagram of the structure at A;

[0034] Figure 6 It is a structural schematic diagram of the movable plate of the present invention sliding on the peripheral side of the upper area ring body;

[0035] Figure 7 It is a structural schematic diagram of the movable plate of the present invention sliding on the peripheral side of the lower area ring body;

[0036] Figure 8 For the present invention Figure 7 Schematic diagram of the structure at B;

[0037] Fig. 9 This is a schematic diagram of the internal space of the fermentation chamber in the early stage of fermentation of the present invention;

[0038] Fig.10 The figure is a schematic diagram of the internal space of the fermentation chamber at the end of the fermentation of the present invention.

[0039] The meaning of each number in the figure is:

[0040] 1. Fermentation equipment; 11. Tank body; 12. Top cover; 13. Support member; 14. Delivery pipe; 15. Middle cavity; 16. Heating plate; 10. Equipment inner cavity; 101. Upper area ring body; 102. Lower area ring body; 103. Drive cavity; 104. Fermentation cavity;

[0041] 2. Cavity adjustment mechanism; 21. Cavity bottom plate; 22. Fixed ring; 23. Movable plate;

[0042] 3. Driving mechanism; 31. Auxiliary parts; 32. Cylinder body; 33. Piston rod;

[0043] 311, fixed plate; 312, inner groove; 313, ring plate;

[0044] 4. Extended page plate;

[0045] 5. Sealing groove; 51. Sealing sleeve. DETAILED DESCRIPTION

[0046] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0047] Example 1, please refer to Figure 1-Figure 3 As shown, the purpose of this embodiment is to provide a microbial flora fermentation process, which includes the following steps:

[0048] Step 1: Fermentation preparation: select microbial strains and raw materials for fermentation, prepare microorganisms, including carbon sources, nitrogen sources, and inorganic salts, and then sterilize;

[0049] Step 2, fermentation: the prepared microorganisms and fermentation raw materials are connected to the fermentation equipment 1 for fermentation;

[0050] Step 3: Separation and purification: After the fermentation is completed, the fermentation products are separated and purified from the microbial cells and unconsumed nutrients;

[0051] Step 4: Concentration and drying: Concentrating and drying the separated and purified product;

[0052] The fermentation equipment 1 includes a tank body 11, a support member 13 is fixedly installed at the bottom of the tank body 11, a top cover 12 is sealed and installed at the top of the tank body 11, a delivery pipe 14 is installed inside the top cover 12, and an equipment cavity 10 is formed inside the tank body 11. A middle cavity 15 is opened inside the inner wall of the equipment cavity 10, and a heating plate 16 is installed inside the middle cavity 15;

[0053] The inner cavity 10 of the device is provided with a cavity adjustment mechanism 2, which includes a cavity bottom plate 21. The cavity bottom plate 21 divides the inner cavity 10 of the device into a driving cavity 103 and a fermentation cavity 104. The inner wall of the fermentation cavity 104 is fixedly provided with an upper region ring body 101 and a lower region ring body 102. The driving cavity 103 is provided with a driving mechanism 3, and the other end of the driving mechanism 3 is connected to the bottom of the cavity bottom plate 21.

[0054] The driving mechanism 3 can drive the cavity bottom plate 21 to slide inside the fermentation chamber 104. When the microbial flora is in the initial fermentation, the cavity bottom plate 21 slides on the upper area ring body 101. When the microbial flora is in the final stage of fermentation, the cavity bottom plate 21 slides on the lower area ring body 102. At the same time, the sliding of the cavity bottom plate 21 can also mix the microbial flora during the fermentation process.

[0055] First, the specific structure of the cavity bottom plate 21 is disclosed. The cavity bottom plate 21 includes a fixed ring 22 and a movable plate 23. The fixed ring 22 is fixedly installed inside the inner cavity 10 of the device, and the movable plate 23 slides inside the fixed ring 22. The outer wall of the movable plate 23 fits with the inner wall of the fixed ring 22, and the top of the movable plate 23 is flush with the top of the fixed ring 22.

[0056] Secondly, the specific structure of the driving mechanism 3 is disclosed. The driving mechanism 3 includes a cylinder body 32 fixedly connected to the bottom of the inner cavity of the driving cavity 103. A piston rod 33 is slidably provided inside the cylinder body 32. The end of the piston rod 33 away from the cylinder body 32 is fixedly connected to the bottom of the movable plate 23. The outer bottom of the movable plate 23 is connected to the auxiliary component 31, and the auxiliary component 31 is sleeved on the outside of the cylinder body 32.

[0057] The auxiliary component 31 includes a fixed plate body 311, the bottom of which is fixedly installed on the bottom of the inner cavity of the driving cavity 103. An inner groove 312 is opened inside the fixed plate body 311, and a ring plate 313 is slidably provided inside the inner groove 312. The top of the ring plate 313 is fixedly connected to the bottom of the movable plate 23.

[0058] The inner end surface of the fixing ring 22 is provided with a sealing groove 5 , the interior of the sealing groove 5 is sealed and fixedly connected with a sealing sleeve 51 , the other end of the sealing sleeve 51 is fixedly mounted on the bottom of the movable plate 23 , and the sealing sleeve 51 is sleeved on the outside of the auxiliary component 31 .

[0059] See also Figure 3 Combined with Figure 4 As shown, the cavity bottom plate 21 is composed of a fixed ring 22 and a movable plate 23. The fixed ring 22 is fixedly installed inside the equipment cavity 10, and the movable plate 23 is located inside the fixed ring 22 and is movably connected to the fixed ring 22. The arrangement of the cavity bottom plate 21 determines the internal space of the fermentation cavity 104. The inner wall of the fermentation cavity 104 is fixedly provided with an upper region ring body 101 and a lower region ring body 102.

[0060] Combination Figure 6 It can be seen that in the initial stage of fermentation, the cylinder body 32 drives the piston rod 33 to slide. Since the bottom of the movable plate 23 is fixedly connected to the end of the piston rod 33, as the cylinder body 32 operates, the movable plate 23 will slide inside the fermentation chamber 104 accordingly. When the movable plate 23 is driven to approach the upper area ring body 101, the bottom of the fermentation chamber 104 is in a lifted state, so that the interior of the fermentation chamber 104 is in a small space state. The smaller space can help the microorganisms quickly adapt to the new fermentation environment, and can enable the microorganisms to more efficiently concentrate on utilizing the surrounding nutrients, laying a good foundation for their rapid growth and metabolism, thereby facilitating the smooth start of the entire fermentation process and the efficient advancement of the initial stage. Fig. 9 As shown, Fig. 9 The shaded portion of the fermentation chamber 104 represents the inner space of the fermentation chamber 104 in the initial stage of fermentation.

[0061] As the fermentation process progresses, the growth and reproduction of microbial flora increases the demand for fermentation space. Figure 7 As shown, when the fermentation enters the final stage, the cylinder body 32 is started again, driving the movable plate 23 connected thereto to move, so that it runs to the vicinity of the lower regional ring body 102. This position change causes the bottom state of the fermentation chamber 104 to change, thereby expanding the overall space of the fermentation chamber 104. The expansion of the space provides more ample discharge space for waste gases such as carbon dioxide generated by microorganisms during metabolism, which effectively prevents the accumulation of waste gases in the fermentation liquid. Since excessive accumulation of waste gases is avoided, adverse effects such as growth inhibition and changes in metabolic pathways caused by waste gas accumulation on the metabolic activities of microorganisms are prevented, thereby ensuring that the microorganisms can maintain a relatively stable and good metabolic state at the end of the fermentation, which is conducive to the continued progress of the fermentation process and the normal generation of fermentation products. Fig.10 As shown, Fig.10 The shaded portion of the fermentation chamber 104 represents the inner space of the fermentation chamber 104 at the final stage of fermentation.

[0062] The inner surface of the fixing ring 22 is provided with a sealing groove 5. Figure 5 and Figure 8It can be seen that a sealing sleeve gasket 51 is installed inside the sealing groove 5, which has an annular structure, one end of which is tightly fixed in the sealing groove 5, and the other end is sealed and fixed to the bottom of the movable plate 23. In this way, when the movable plate 23 is slid, the movable plate 23 and the fixed ring 22 can always maintain a sealed connection state, which effectively guarantees the sealing of the fermentation chamber 104. At the same time, the sealing sleeve gasket 51 is preferably made of rubber material. Rubber has good heat resistance and corrosion resistance, and can cope with the high temperature environment and the corrosive effects of substances such as fermentation liquid during the fermentation process, thereby stably maintaining its sealing performance during long-term use, ensuring the stable operation of the entire fermentation system, and creating a reliable closed space environment for microbial fermentation.

[0063] When the movable plate 23 is adjusted in position in the fermentation chamber 104, the ring plate 313 fixedly connected to the bottom thereof will slide inside the fixed plate body 311 as the movable plate 23 moves. This close connection and matching mode provides reliable stability support for the sliding of the movable plate 23, ensuring that the movable plate 23 will not be unstable such as shaking or deflecting during the movement, thereby ensuring the accuracy and effectiveness of the adjustment of the internal space of the fermentation chamber 104.

[0064] At the same time, the ring plate 313 and the sealing sleeve 51 are both sleeved on the outside of the cylinder body 32, and can also provide further protection for the cylinder body 32. On the one hand, the ring plate 313 can prevent foreign matter from entering from the connection between the movable plate 23 and the fixed plate body 311, thereby avoiding physical damage to the cylinder body 32. On the other hand, the sealing performance of the sealing sleeve 51 can effectively prevent the fermentation liquid, gas and other possible corrosive substances from contacting the cylinder body 32, reducing the risk of chemical erosion, thereby extending the service life of the cylinder body 32 and ensuring the stability and reliability of the entire fermentation equipment 1 during long-term operation.

[0065] During the entire process of microbial fermentation, the cylinder body 32 is always in a driven state, driving the movable plate 23 to perform small-amplitude and high-frequency sliding movements around the upper area ring body 101 or the lower area ring body 102. Through such movement, the microbial flora and the fermentation liquid can be effectively mixed, and the fermentation reaction can be carried out more evenly and efficiently. Compared with the method of stirring with a stirring rod in traditional technology, this structure can effectively reduce the generation of bubbles during the mixing process, creating a more stable fermentation environment for the microorganisms. At the same time, this mixing method greatly reduces the mechanical shear force on the microbial flora, reduces the reduction in the number and performance degradation of the microbial flora due to physical damage, and effectively guarantees the quality and efficiency of microbial fermentation.

[0066] The above-mentioned cylinder body 32 adopts a multi-stage cylinder structure. As is well known to those skilled in the art, the cylinder body 32 is composed of multiple piston-cylinder units connected in sequence. During operation, the gas first enters the first piston-cylinder unit to push the piston of the unit to move. When the piston moves to the end of the stroke, the gas enters the next-stage piston-cylinder unit through the internal passage to drive the next-stage piston to continue moving. This multi-stage driving method can achieve a longer stroke in a smaller space, so that the cylinder body 32 can output a greater thrust or pull, and the action sequence and movement speed of different levels of piston cylinders can be controlled according to actual needs to meet the requirements of accurately controlling the position of the movable plate 23 in the fermentation equipment 1.

[0067] In order to further enhance the mixing effect on the microbial flora, an extension leaf plate 4 is fixedly installed on the top of the movable plate 23 , and a plurality of flow holes are formed on the outer surface of the extension leaf plate 4 .

[0068] The extension plate 4 is made of elastic material.

[0069] The improvement is: Figure 3 As shown, an extension plate 4 (made of rubber) is fixedly installed on the top of the movable plate 23. When the extension plate 4 slides in the fermentation liquid along with the movable plate 23, its elasticity can buffer the collision force between the extension plate 4 and the bacterial colony. If the extension plate 4 encounters the bacterial colony, the elastic material will deform appropriately instead of rigidly squeezing the bacterial colony, thereby protecting the integrity of the bacterial colony to the greatest extent.

[0070] from Figure 2 It can be seen that the outer surface of the extension plate 4 is provided with a plurality of flow holes. When the fermentation liquid is mixed, the fermentation liquid flows through these flow holes. This flow mode makes the flow of the fermentation liquid more complex and dispersed, avoiding the formation of a local high-pressure area due to the movement of the extension plate 4, thereby effectively preventing the squeezing of the bacterial colony. Moreover, the flow of liquid through the flow holes can further promote the mixing uniformity of the fermentation liquid, so that the microbial flora can better contact nutrients and oxygen, thereby improving the fermentation efficiency.

[0071] At the same time, the extended page plate 4 will not contact the upper area ring body 101 and the lower area ring body 102 during the sliding process, so that the sliding of the extended page plate 4 is not hindered, providing a strong guarantee for the smooth progress of the entire fermentation process.

[0072] Embodiment 2, the present invention also provides a method for preparing a microbial flora, comprising the following steps:

[0073] Step 1: strain selection: select the original strain;

[0074] Step 2: Slant culture: inoculate the bacteria onto the slant culture medium to activate the bacteria;

[0075] Step 3: Seed liquid preparation: pick the activated strains and inoculate them into the seed culture medium;

[0076] Step 4: Fermentation: fermenting the microbial flora using the fermentation process provided in Example 1.

[0077] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and descriptions are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A microbial flora fermentation process, characterized in that: This process includes the following steps: S1.1, Fermentation preparation: Select microbial strains and raw materials for fermentation, prepare microorganisms, including carbon source, nitrogen source, inorganic salts, and then sterilize; S1.2, fermentation: placing the prepared microorganisms and fermentation raw materials into a fermentation device (1) for fermentation; S1.3, separation and purification: after fermentation, the fermentation products are separated and purified from the microbial cells and unconsumed nutrients; S1.4, Concentration and drying: Concentrate and dry the separated and purified product; The fermentation equipment (1) comprises a tank body (11), a support member (13) is fixedly mounted on the bottom of the tank body (11), a top cover (12) is sealedly mounted on the top of the tank body (11), a delivery pipe (14) is mounted inside the top cover (12), an equipment inner cavity (10) is formed inside the tank body (11), a middle cavity (15) is formed inside the inner wall of the equipment inner cavity (10), and a heating plate (16) is mounted inside the middle cavity (15); A cavity adjustment mechanism (2) is arranged inside the inner cavity (10) of the device, and the cavity adjustment mechanism (2) comprises a cavity bottom plate (21), and the cavity bottom plate (21) divides the inner cavity (10) of the device into a driving cavity (103) and a fermentation cavity (104), and an upper region ring body (101) and a lower region ring body (102) are fixedly installed on the inner wall of the fermentation cavity (104), and a driving mechanism (3) is installed inside the driving cavity (103), and the other end of the driving mechanism (3) is connected to the bottom of the cavity bottom plate (21); The driving mechanism (3) can drive the cavity bottom plate (21) to slide inside the fermentation cavity (104). When the microbial flora is in the initial stage of fermentation, the cavity bottom plate (21) slides on the upper region ring body (101). When the microbial flora is in the final stage of fermentation, the cavity bottom plate (21) slides on the lower region ring body (102). At the same time, the sliding of the cavity bottom plate (21) can also mix the microbial flora during the fermentation process.

2. The microbial flora fermentation process according to claim 1, characterized in that: The cavity bottom plate (21) comprises a fixed ring (22) and a movable plate (23); the fixed ring (22) is fixedly installed inside the inner cavity (10) of the device; the movable plate (23) slides inside the fixed ring (22); the outer wall of the movable plate (23) is in contact with the inner wall of the fixed ring (22); and the top of the movable plate (23) is flush with the top of the fixed ring (22).

3. The microbial flora fermentation process according to claim 2, characterized in that: The driving mechanism (3) comprises a cylinder body (32) fixedly connected to the bottom of the inner cavity of the driving cavity (103); a piston rod (33) is slidably provided inside the cylinder body (32); one end of the piston rod (33) away from the cylinder body (32) is fixedly connected to the bottom of the movable plate (23); the outer bottom of the movable plate (23) is connected to the auxiliary component (31); and the auxiliary component (31) is sleeved on the outside of the cylinder body (32).

4. The microbial flora fermentation process according to claim 3, characterized in that: The auxiliary component (31) includes a fixed plate body (311), the bottom of which is fixedly installed on the bottom of the inner cavity of the driving cavity (103), an inner groove (312) is opened inside the fixed plate body (311), a ring plate (313) is slidably provided inside the inner groove (312), and the top of the ring plate (313) is fixedly connected to the bottom of the movable plate (23).

5. The microbial flora fermentation process according to claim 3, characterized in that: The inner end surface of the fixing ring (22) is provided with a sealing groove (5), the interior of the sealing groove (5) is sealed and fixedly connected with a sealing sleeve (51), the other end of the sealing sleeve (51) is fixedly mounted on the bottom of the movable plate (23), and the sealing sleeve (51) is sleeved on the outside of the auxiliary component (31).

6. The microbial flora fermentation process according to claim 3, characterized in that: An extension leaf plate (4) is fixedly mounted on the top of the movable plate (23), and a plurality of flow holes are formed on the outer surface of the extension leaf plate (4).

7. The microbial flora fermentation process according to claim 6, characterized in that: The extension page plate (4) is made of elastic material.

8. A method for preparing a microbial flora, characterized in that: The following steps are involved: S2.

1. Selection of strains: Select the original strains; S2.2, slant culture: inoculate the bacteria onto the slant culture medium to activate the bacteria; S2.3, seed solution preparation: pick the activated strains and inoculate them into the seed culture medium; S2.4, fermentation: fermenting the microbial flora using the fermentation process described in claim 1.

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