Automatic dyeing mechanism of biological tissue sample section dyeing equipment

Abstract

The invention provides an automatic dyeing mechanism of biological tissue sample section dyeing equipment, which comprises an automatic dyeing glass slide feeding and clamping assembly, an automatic dyeing reaction disc and an automatic dyeing glass slide discharging and clamping assembly, a main console of the biological tissue sample section dyeing equipment is in control connection with an automatic dyeing glass slide feeding and clamping assembly, an automatic dyeing reaction disc and an automatic dyeing glass slide discharging and clamping assembly, the automatic dyeing reaction disc is provided with a plurality of dyeing grooves, and the dyeing grooves can be filled with dyeing reagents and are matched with microscope glass slides. The automatic dyeing glass slide feeding and clamping assembly and the automatic dyeing glass slide discharging and clamping assembly are both matched with the feeding and discharging buffering mechanism, the feeding and discharging buffering mechanism can be used for placing a plurality of microscope glass slide vertical placing supports in an array mode, and a plurality of microscope glass slides can be vertically and fixedly placed in the microscope glass slide vertical placing supports in an array mode. The microscope glass slide dyeing tank has the beneficial effects that the microscope glass slides can be separated by the dyeing tank for dyeing operation, so that cross contamination is avoided.

Description

Technical Field

[0001] The invention relates to the technical field of biological tissue sample slice staining, and in particular to an automatic staining mechanism of biological tissue sample slice staining equipment. Background Art

[0002] In the existing biological sample analysis technology, the analysis methods commonly used include microscopic analysis, microarray analysis (e.g., protein and nucleic acid microarray analysis) and mass spectrometry. Preparing samples for these and other types of analysis usually includes: contacting the biological sample with a series of processing liquids. Some of these processing liquids (e.g., staining reagents and counterstaining reagents) can add colors and, in contrast or on the contrary, change the visual characteristics of invisible or difficult-to-see sample components (e.g., at least some types of cells and intracellular structures). Other processing liquids (e.g., deparaffinized liquids) can be used to achieve other processing purposes. If a variety of processing liquids are used to process the sample, the application and subsequent removal of various processing liquids can be very important for producing samples suitable for analysis. In some cases, processing samples with a variety of processing liquids includes: manually applying the processing liquids to microscope slides that carry the samples respectively. However, this method of manually processing samples tends to be labor-intensive and imprecise, so biological tissue sample section staining equipment has emerged.

[0003] The automatic staining and processing technology of biological tissue sample sections is an important step in the entire process of automated staining, drying and sealing of biological tissue samples. It is also an indispensable step. In order to make the processed biological tissue samples have an appearance that is easy to observe and to maintain the consistency and efficiency of the processing process, the staining step is an object that must be strictly controlled during the processing of biological tissue samples. The purpose of staining is to make different structures in cell tissues present different colors for easy observation. The classic hematoxylin and eosin (eosin) staining method is a routine staining for histological specimens and pathological section specimens, referred to as HE staining. After staining, the cell nucleus is stained purple-blue by hematoxylin, and most cytoplasm and non-cellular components are stained pink by eosin.

[0004] Existing biological tissue sample section staining equipment can replace manual staining. These machines automatically process samples by immersing racks carrying batches of microscope slides in an open bath of processing liquid. However, the existing biological tissue sample section staining equipment places batches of microscope slides containing biological tissue sample sections horizontally in a slide carrier box. Although there is a certain upper and lower space interval between each microscope slide, a whole box of microscope slides is processed together during the immersion staining operation. When the intervals are immersed in a common staining reagent, it is inevitable that there is a risk of cross-contamination between microscope slides in the same box. For example, cells of biological tissue sample sections can leave the sample on one microscope slide and move to another microscope slide in the common staining reagent. This form of contamination greatly reduces the accuracy of certain types of sample analysis. Moreover, after a whole box of horizontally placed microscope slides are immersed in a common staining reagent, the osmotic pressure of the staining reagent applied to the upper microscope slide is lower than that applied to the lower microscope slide, inevitably leading to the problem of uneven staining of the upper and lower microscope slides, resulting in poor product quality consistency of the final stained biological tissue sample section microscope slides, and bringing a very bad user experience. Summary of the invention

[0005] In order to solve the problems in the prior art, the present invention provides an automatic staining mechanism for a biological tissue sample slice staining device. By arranging an automatic staining slide feeding clamping component, an automatic staining reaction tray and an automatic staining slide discharging clamping component that cooperate with each other in the automatic staining mechanism of the biological tissue sample slice staining device, the staining slot of the automatic staining reaction tray can separate and isolate each microscope slide for staining operations, thereby avoiding the risk of cross contamination between microscope slides containing biological tissue sample slices, improving user experience, and solving the problems of cross contamination risk and poor product consistency when the biological tissue sample slice staining device is stained in the prior art.

[0006] The present invention provides an automatic staining mechanism for a biological tissue sample section staining device, which cooperates with a loading and unloading buffer mechanism of the biological tissue sample section staining device, and comprises an automatic staining slide feeding clamping assembly, an automatic staining reaction tray and an automatic staining slide discharging clamping assembly, wherein the automatic staining slide feeding clamping assembly, the automatic staining reaction tray and the automatic staining slide discharging clamping assembly are all fixedly arranged on a workbench, and a main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining slide feeding clamping assembly, the automatic staining reaction tray and the automatic staining slide discharging clamping assembly, and the automatic staining reaction tray is provided with a plurality of staining slots for staining operations of a single microscope slide, and the staining slots can be filled with dye The automatic staining slide feeding and clamping assembly is compatible with the microscope slide, the automatic staining slide discharging and clamping assembly are both coordinated with the loading and unloading buffer mechanism, the loading and unloading buffer mechanism can place a plurality of microscope slide vertical brackets in an array, the microscope slide vertical bracket can vertically and fixedly place a plurality of microscope slides in an array and each of the microscope slides is separated, the automatic staining slide feeding and clamping assembly can clamp the microscope slide in the microscope slide vertical bracket on the loading and unloading buffer mechanism and move it into the staining tank for staining operation, and after the microscope slide is stained and put back into the microscope slide vertical bracket, the discharging is completed through the loading and unloading buffer mechanism.

[0007] The present invention is further improved in that a reaction disc rotating shaft is provided in the middle of the automatic staining reaction disc, and the automatic staining reaction disc is fixedly connected to the top of the reaction disc rotating shaft.

[0008] The present invention is further improved in that a reaction disc rotation drive motor is provided at the bottom of the reaction disc shaft, the reaction disc rotation drive motor is fixedly arranged on a workbench, and the reaction disc rotation drive motor is rotationally drivenly connected to the reaction disc shaft.

[0009] The present invention is further improved, the automatic staining slide feeding and clamping assembly includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft and an automatic staining slide feeding manipulator, the automatic staining slide feeding motor is fixedly arranged on the workbench, the automatic staining slide feeding motor is rotationally driven connected to the bottom of the automatic staining slide feeding shaft, the top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding manipulator, and the automatic staining slide feeding manipulator can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction tray.

[0010] The present invention is further improved, the automatic staining glass slide discharging and clamping assembly includes an automatic staining glass slide discharging motor, an automatic staining glass slide discharging shaft and an automatic staining glass slide discharging manipulator, the automatic staining glass slide discharging motor is fixedly arranged on the workbench, the automatic staining glass slide discharging motor is rotationally driven connected to the bottom of the automatic staining glass slide discharging shaft, the top of the automatic staining glass slide discharging shaft is fixedly connected to the top of the automatic staining glass slide discharging manipulator, and the automatic staining glass slide discharging manipulator can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction tray.

[0011] The present invention is further improved and also includes a reagent adding mechanism, which cooperates with the automatic staining reaction disk, and the main control console of the biological tissue sample section staining device is control-connected to the reagent adding mechanism, and the reagent adding mechanism can add staining reagent into the staining tank.

[0012] The present invention is further improved and also includes a staining tank cleaning component, which cooperates with the automatic staining reaction disk, and the main control console of the biological tissue sample section staining equipment is control-connected to the staining tank cleaning component, and the staining tank cleaning component can extract the staining reagent in the staining tank.

[0013] The present invention is further improved and also includes a slide discharging and cleaning component, which cooperates with the automatic staining slide discharging robot and can clean the microscope slides that have completed the staining operation.

[0014] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention provides an automatic staining mechanism for a biological tissue sample section staining device, by arranging an automatic staining slide feeding clamping component, an automatic staining reaction tray and an automatic staining slide discharging clamping component that cooperate with each other in the automatic staining mechanism of the biological tissue sample section staining device, the automatic staining slide feeding clamping component can clamp the microscope slide in the microscope slide vertical placement bracket on the loading and unloading buffer mechanism and move it into the staining tank for staining operation, and after the microscope slide is stained, it is put back into the microscope slide vertical placement bracket and then completed by the loading and unloading buffer mechanism. The dyeing slot of the automatic dyeing reaction tray can separate and isolate each microscope slide for dyeing, avoiding the risk of cross-contamination between microscope slides containing biological tissue sample slices, and each microscope slide immersed in a common dyeing reagent is subjected to the same osmotic pressure of the dyeing reagent. Finally, the dyed biological tissue sample slice microscope slides have better product quality consistency, which improves the product quality of the biological tissue sample slice microscope slides, improves the user experience, and solves the problems of cross-contamination risk and poor product consistency when dyeing biological tissue sample slice staining equipment in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 It is a structural diagram of the automatic staining mechanism of the biological tissue sample slice staining device of the present invention.

[0016] In the figure, 2-loading and loading buffer mechanism, 3-automatic staining mechanism, 31-automatic staining slide feeding and clamping assembly, 32-automatic staining reaction tray, 321-staining tank, 33-automatic staining slide discharging and clamping assembly, 4-reagent adding mechanism, 35-staining tank cleaning assembly, 36-slide discharging and cleaning assembly. DETAILED DESCRIPTION

[0017] The present invention is further described in detail below with reference to the accompanying drawings and embodiments.

[0018] like Figure 1As shown, an automatic staining mechanism 3 of a biological tissue sample section staining device provided by the present invention cooperates with a loading and unloading buffer mechanism 2 of the biological tissue sample section staining device, including an automatic staining slide feeding clamping component 31, an automatic staining reaction tray 32 and an automatic staining slide discharging clamping component 33. The automatic staining slide feeding clamping component 31, the automatic staining reaction tray 32 and the automatic staining slide discharging clamping component 33 are all fixedly arranged on a workbench. The main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining slide feeding clamping component 31, the automatic staining reaction tray 32 and the automatic staining slide discharging clamping component 33. The automatic staining reaction tray 32 is provided with a plurality of staining units for a single microscope slide staining operation. Slot 321, the staining slot 321 can be filled with staining reagent and is compatible with microscope slides, the automatic staining slide feeding clamping component 31 and the automatic staining slide discharging clamping component 32 are both coordinated with the loading and unloading buffer mechanism 2, the loading and unloading buffer mechanism 2 can place multiple microscope slide vertical brackets in an array, the microscope slide vertical bracket can vertically fix multiple microscope slides in an array and each microscope slide is separated, a reaction disc rotating shaft is provided in the middle of the automatic staining reaction disc 32, the automatic staining reaction disc 32 is fixedly connected to the top of the reaction disc rotating shaft, a reaction disc rotating drive motor is provided at the bottom of the reaction disc rotating shaft, the reaction disc rotating drive motor is fixedly set on the workbench, and the reaction disc rotating drive motor is connected to the reaction disc rotating shaft for rotation driving. In this embodiment, the automatic staining slide feeding and clamping assembly can clamp the microscope slide in the microscope slide vertical holder on the loading and unloading buffer mechanism and move it into the staining tank for staining operation, and after the microscope slide is stained, it is put back into the microscope slide vertical holder and then discharged through the loading and unloading buffer mechanism. The staining tank of the automatic staining reaction tray can separate each microscope slide for staining operation, avoiding the risk of cross contamination between microscope slides containing biological tissue sample slices, and each microscope slide immersed in the common staining reagent is subjected to the same osmotic pressure of the staining reagent. Finally, the quality consistency of the biological tissue sample slice microscope slide product is better, which improves the product quality of the biological tissue sample slice microscope slide and improves the user experience.

[0019] like Figure 1As shown, the automatic staining slide feeding and gripping assembly 31 includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft and an automatic staining slide feeding manipulator. The automatic staining slide feeding motor is fixedly arranged on the workbench, the automatic staining slide feeding motor is connected to the bottom of the automatic staining slide feeding shaft for rotational driving, the top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding manipulator, and the automatic staining slide feeding manipulator can reciprocate between the loading and unloading buffer mechanism 2 and the automatic staining reaction disk 32. In this embodiment, the automatic staining slide feeding manipulator can, under the drive of the automatic staining slide feeding motor, clamp the microscope slide in the vertically placed bracket of the microscope slide on the loading and unloading buffer mechanism and move it into the staining tank for staining.

[0020] like Figure 1 As shown, the automatic staining slide discharging and gripping assembly 33 includes an automatic staining slide discharging motor, an automatic staining slide discharging shaft and an automatic staining slide discharging manipulator. The automatic staining slide discharging motor is fixedly arranged on the workbench, the automatic staining slide discharging motor is connected to the bottom of the automatic staining slide discharging shaft for rotational driving, the top of the automatic staining slide discharging shaft is fixedly connected to the top of the automatic staining slide discharging manipulator, and the automatic staining slide discharging manipulator can reciprocate between the loading and unloading buffer mechanism 2 and the automatic staining reaction tray 32. In this embodiment, the automatic staining slide discharging manipulator can, under the drive of the automatic staining slide discharging motor, grip the microscope slide that has completed the staining operation in the staining tank and move it into the microscope slide vertical support on the loading and unloading buffer mechanism.

[0021] like Figure 1 As shown, it also includes a reagent adding mechanism 4, which cooperates with the automatic staining reaction disk 32, and the main control console of the biological tissue sample section staining device is controlled and connected with the reagent adding mechanism 4, and the reagent adding mechanism 4 can add staining reagent into the staining tank 321. In this embodiment, the reagent adding mechanism can add staining reagent into the staining tank according to the control instruction of the main control console of the biological tissue sample section staining device.

[0022] like Figure 1 As shown, the staining tank cleaning component 35 is also included, and the staining tank cleaning component 35 cooperates with the automatic staining reaction disk 32. The main control console of the biological tissue sample section staining device is controlled and connected with the staining tank cleaning component 35, and the staining tank cleaning component 35 can extract the staining reagent in the staining tank 321. In this embodiment, the staining tank cleaning component can extract the staining reagent in the staining tank according to the control instruction of the main control console of the biological tissue sample section staining device.

[0023] like Figure 1As shown, the slide discharging and cleaning assembly 36 is also included. The slide discharging and cleaning assembly 36 cooperates with the automatic staining slide discharging manipulator, and the slide discharging and cleaning assembly 36 can clean the microscope slides that have completed the staining operation. In this embodiment, the slide discharging and cleaning assembly is filled with cleaning reagents, and the microscope slides that have completed the staining operation can be cleaned by the slide discharging and cleaning assembly before being discharged. During the staining operation, the microscope slides can be placed in the slide discharging and cleaning assembly for cleaning each time after the staining operation in the staining tank, and then placed in the next staining tank for staining according to the staining process.

[0024] As can be seen from the above, the automatic staining mechanism of a biological tissue sample section staining device provided by the present invention is provided with an automatic staining slide feeding clamping component, an automatic staining reaction tray and an automatic staining slide discharging clamping component that cooperate with each other in the automatic staining mechanism of the biological tissue sample section staining device. The automatic staining slide feeding clamping component can clamp the microscope slide in the microscope slide vertical placement bracket on the loading and unloading buffer mechanism and move it into the staining tank for staining operation. After the microscope slide is stained, it is put back into the microscope slide vertical placement bracket and then discharged through the loading and unloading buffer mechanism, and the automatic staining is completed. The staining slot of the reaction disk can separate and isolate each microscope slide for staining operations, avoiding the risk of cross-contamination between microscope slides containing biological tissue sample slices, and each microscope slide immersed in a common staining reagent is subjected to the same osmotic pressure of the staining reagent. Finally, the biological tissue sample slice microscope slides stained have better product quality consistency, which improves the product quality of the biological tissue sample slice microscope slides, improves the user experience, and solves the problems of cross-contamination risks and poor product consistency when staining biological tissue sample slice staining equipment in the prior art.

[0025] The specific implementation modes described above are preferred implementation modes of the present invention, and are not intended to limit the specific implementation scope of the present invention. The scope of the present invention includes but is not limited to the specific implementation modes, and all equivalent changes made according to the present invention are within the protection scope of the present invention.

Claims

1. An automatic staining mechanism of a biological tissue sample section staining device, which cooperates with a loading and unloading buffer mechanism of the biological tissue sample section staining device, and is characterized by: The invention comprises an automatic staining slide feeding and clamping assembly, an automatic staining reaction tray and an automatic staining slide discharging and clamping assembly, wherein the automatic staining slide feeding and clamping assembly, the automatic staining reaction tray and the automatic staining slide discharging and clamping assembly are all fixedly arranged on a workbench, and a main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining slide feeding and clamping assembly, the automatic staining reaction tray and the automatic staining slide discharging and clamping assembly, the automatic staining reaction tray is provided with a plurality of staining slots for staining operations of a single microscope slide, the staining slots can be filled with staining reagents and are compatible with microscope slides, ... are fixedly arranged on a workbench, and a main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining slide feeding and clamping assembly, the automatic staining reaction tray and the automatic staining slide discharging and clamping assembly are controlled and connected, the automatic staining reaction tray and the automatic staining reaction tray are provided with a plurality of staining slots for staining operations of a single microscope slide, the staining slots can be filled with staining reagents and are compatible with microscope slides, the automatic staining slide feeding and clamping assembly are fixedly arranged on a workbench, and a main control console of the biological tissue sample section staining device is controlled and connected with the automatic staining slide feeding and clamping assembly, the automatic staining reaction tray and the automatic staining slide discharging and clamping assembly are controlled and The clamping component and the automatic staining slide discharging clamping component are both coordinated with the loading and unloading buffer mechanism. The loading and unloading buffer mechanism can place multiple microscope slide vertical stands in an array. The microscope slide vertical stands can vertically and fixedly place multiple microscope slides in an array and each of the microscope slides is separated from each other. The automatic staining slide feeding clamping component can clamp the microscope slides in the microscope slide vertical stand on the loading and unloading buffer mechanism and move them into the staining tank for staining. After the microscope slides are stained, they are put back into the microscope slide vertical stand and then discharged through the loading and unloading buffer mechanism.

2. The automatic staining mechanism of the biological tissue sample section staining device according to claim 1, characterized in that: A reaction disc rotating shaft is arranged in the middle of the automatic staining reaction disc, and the automatic staining reaction disc is fixedly connected to the top of the reaction disc rotating shaft.

3. The automatic staining mechanism of the biological tissue sample section staining device according to claim 2, characterized in that: A reaction disc rotation drive motor is provided at the bottom of the reaction disc shaft. The reaction disc rotation drive motor is fixedly arranged on a workbench and is rotationally drivenly connected to the reaction disc shaft.

4. The automatic staining mechanism of the biological tissue sample section staining device according to claim 3, characterized in that: The automatic staining slide feeding and clamping assembly includes an automatic staining slide feeding motor, an automatic staining slide feeding shaft and an automatic staining slide feeding manipulator. The automatic staining slide feeding motor is fixedly arranged on a workbench, the automatic staining slide feeding motor is rotationally driven connected to the bottom of the automatic staining slide feeding shaft, the top of the automatic staining slide feeding shaft is fixedly connected to the top of the automatic staining slide feeding manipulator, and the automatic staining slide feeding manipulator can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction tray.

5. The automatic staining mechanism of the biological tissue sample section staining device according to claim 4, characterized in that: The automatic staining glass slide discharging and clamping assembly includes an automatic staining glass slide discharging motor, an automatic staining glass slide discharging shaft and an automatic staining glass slide discharging manipulator. The automatic staining glass slide discharging motor is fixedly arranged on a workbench, the automatic staining glass slide discharging motor is rotationally driven connected to the bottom of the automatic staining glass slide discharging shaft, the top of the automatic staining glass slide discharging shaft is fixedly connected to the top of the automatic staining glass slide discharging manipulator, and the automatic staining glass slide discharging manipulator can reciprocate between the loading and unloading buffer mechanism and the automatic staining reaction tray.

6. The automatic staining mechanism of the biological tissue sample section staining device according to claim 5, characterized in that: It also includes a reagent adding mechanism, which cooperates with the automatic staining reaction disk. The main control console of the biological tissue sample slice staining device is control-connected to the reagent adding mechanism, and the reagent adding mechanism can add staining reagent into the staining tank.

7. The automatic staining mechanism of the biological tissue sample section staining device according to claim 6, characterized in that: It also includes a staining tank cleaning component, which cooperates with the automatic staining reaction disk. The main control console of the biological tissue sample section staining device is control-connected to the staining tank cleaning component, and the staining tank cleaning component can extract the staining reagent in the staining tank.

8. The automatic staining mechanism of the biological tissue sample section staining device according to claim 7, characterized in that: It also includes a slide discharging and cleaning component, which cooperates with the automatic staining slide discharging robot, and the slide discharging and cleaning component can clean the microscope slides that have completed the staining operation.

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