Premixed feed additive for laying hens and preparation method thereof

Abstract

The invention discloses a premix feed additive for laying hens and a preparation method thereof, and the premix feed additive for laying hens is prepared from the following raw materials in parts by weight: 10-20 parts of allicin, 30-50 parts of yeast cell wall polysaccharide, 50-80 parts of kelp powder, 20-40 parts of radix astragali extract, 10-30 parts of humic acid, 5-10 parts of zinc methionine and 2-8 parts of selenium powder. 5-10 parts of a probiotic metabolite and 2-5 parts of a plant essential oil compound. The preparation method of the premix feed additive for laying hens comprises the following preparation processes: S1, preparing allicin; s2, preparing yeast cell wall polysaccharide; s3, preparing kelp powder; s4, preparing a radix astragali extract; s5, preparing humic acid; s6, preparing methionine; s7, preparing selenium powder; and S8, preparing a probiotic metabolite. The additive provided by the invention can realize nutrition balance, meet the nutritional requirements of the laying hens in all stages of growth and egg laying, and improve the feed utilization rate, thereby improving the egg yield of the laying hens and the quality of eggs.

Description

Technical Field

[0001] The invention relates to the technical field of animal feed, and in particular to a laying hen premix feed additive and a preparation method thereof. Background Art

[0002] In the laying hen farming industry, the quality of feed plays a decisive role in the healthy growth, egg-laying performance and egg quality of laying hens. Traditional laying hen feeds often only focus on the supply of basic nutrients, which is difficult to meet the needs of laying hens for balanced nutrition, immune enhancement and health protection under the modern large-scale and intensive farming model.

[0003] At present, although some laying hen feed additives on the market can improve the production performance of laying hens to a certain extent, they have many limitations. For example, some additives have a single ingredient and only focus on one aspect of efficacy, such as simply increasing egg production, but ignore the improvement of laying hen immunity, resulting in weak resistance of laying hens when facing disease invasion, which is easy to cause various diseases and increase breeding costs.

[0004] In addition, the preparation process of some additives is not scientific and advanced enough. For example, the preparation process of some extracts fails to fully consider the active retention of the effective ingredients, which greatly reduces the effectiveness of the additives in practical applications. Moreover, during the mixing process of some additives, due to unreasonable mixing equipment and processes, the ingredients are mixed unevenly, which affects the role of the additives in the feed, and thus cannot stably provide comprehensive and balanced nutritional support and health protection for laying hens. Summary of the invention

[0005] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a laying hen premix feed additive and a preparation method thereof.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A premixed feed additive for laying hens, comprising the following raw materials in weight components:

[0008] 10-20 parts of allicin, 30-50 parts of yeast cell wall polysaccharide, 50-80 parts of kelp powder, 20-40 parts of astragalus extract, 10-30 parts of humic acid, 5-10 parts of zinc methionine, 2-8 parts of selenium powder, 5-10 parts of probiotic metabolites, 2-5 parts of plant essential oil complex.

[0009] A method for preparing a premixed feed additive for laying hens, comprising the following preparation process:

[0010] S1, preparing allicin, chopping fresh garlic, putting it into a distillation apparatus, adding water to carry out water distillation, collecting the distillate, and then extracting the distillate with an organic solvent to obtain an organic phase containing allicin;

[0011] S2, preparing yeast cell wall polysaccharides, selecting yeast strains with high polysaccharide production, inoculating them into glucose liquid culture medium, placing them into a conventional fermentation tank, and carrying out deep fermentation culture for 48-72 hours at 28-30°C and pH 5.5-6.5. After the fermentation is completed, centrifuging the fermentation broth, collecting the precipitate, adding ammonium sulfate solution to the precipitate for salting out to precipitate the polysaccharide, and then dissolving the precipitate in an appropriate amount of buffer solution, and dialyzing through a dialysis bag to remove salt;

[0012] S3, preparing kelp powder, washing fresh kelp, removing surface impurities, putting it into a low-temperature dryer, and drying it at 40-50°C until the moisture content is less than 10%. Then use a mechanical grinding device to grind the dried kelp into coarse powder, and then put the coarse kelp powder into a ball mill or other grinding and refining equipment for further crushing;

[0013] S4, preparing an astragalus extract, crushing the astragalus into particles of appropriate size, putting them into a round-bottom flask, adding an appropriate amount of ethanol, the mass ratio of ethanol to astragalus being 8:1, connecting a reflux condenser, reflux extraction at 70-80° C. for 2-3 times, each extraction for 1-2 hours, and extracting the solution, removing ethanol by vacuum distillation to obtain an astragalus extract;

[0014] S5, preparing humic acid, putting humic acid into a reaction container, adding an oxidant, and controlling the temperature at 60-80° C. under acidic or alkaline conditions according to the type of the selected oxidant, stirring the reaction for 3-4 hours, and performing chemical oxidation on the surface of the humic acid. After the reaction is completed, dissolving the modified humic acid in an alkaline aqueous solution, slowly adding an electrolyte solution, and under stirring conditions, allowing the humic acid to reconstruct its structure through solution self-assembly to form an active micro-region;

[0015] S6, preparing methionine, dissolving methionine and an appropriate amount of zinc salt in an appropriate amount of water, and slowly adding the zinc salt solution dropwise to the methionine solution under stirring, while adjusting the pH value of the reaction system to maintain it at 6-7, controlling the reaction temperature at 40-50° C., reacting for 3-4 hours, and filtering, washing, and drying the product after the reaction is completed;

[0016] S7, preparing selenium powder, in a reaction container, dissolving sodium selenite in an appropriate amount of water, adding an appropriate amount of reducing agent, controlling the reaction temperature at 30-40° C. under stirring conditions, reacting for 1-2 hours, so that the sodium selenite is reduced to selenium powder;

[0017] S8, preparing probiotic metabolites, screening specific probiotic strains, inoculating them into a liquid culture medium containing specific nutrients, placing them in a shake flask, and fermenting them in the shake flask for 24-48 hours under suitable temperature and rotation speed conditions. After the fermentation is completed, the fermentation liquid is centrifuged and the supernatant is collected;

[0018] S9, preparing a plant essential oil complex, selecting thyme essential oil and oregano essential oil, mixing them with the wall material solution in a certain proportion, forming an emulsion under high-speed stirring, and passing the emulsion through a spray drying device, spray drying under the conditions of an inlet air temperature of 150-180° C. and an outlet air temperature of 80-100° C., to prepare a plant essential oil complex;

[0019] S10, special treatment, putting all the pre-treated raw materials into a plasma treatment device, passing nitrogen gas at 50-80°C and 10-20 Pa, and treating for 5-10 minutes;

[0020] S11, fully mixing: put the raw materials weighed in proportion into a common V-type mixer for full mixing.

[0021] Preferably, the organic solvent in step S1 is diethyl ether, and during the extraction process, the volume ratio of diethyl ether to distillate is 1:2-1:3, and the number of extractions is 2-3 times.

[0022] Preferably, in step S2, the glucose concentration in the glucose liquid culture medium is 10-15 g / L, the yeast powder concentration is 5-8 g / L, and the peptone concentration is 5-8 g / L.

[0023] Preferably, in step S3, when the kelp coarse powder is further crushed in a ball mill or other grinding and refining equipment, the grinding time is 30-60 minutes, and the particle size of the kelp powder reaches 100-200 mesh by adjusting the grinding time and the ratio of the grinding medium.

[0024] Preferably, in step S5, when hydrogen peroxide is used as the oxidant, the mass ratio of humic acid to hydrogen peroxide is 1:0.5-1:1, and the pH value is controlled at 3-4 under acidic conditions; when potassium permanganate is used as the oxidant, the mass ratio of humic acid to potassium permanganate is 1:0.3-1:0.5, and the pH value is controlled at 9-10 under alkaline conditions to optimize the surface modification effect of humic acid.

[0025] Preferably, in step S8, the specific nutrients include 10-15 g / L glucose, 5-8 g / L peptone, 5-8 g / L yeast powder, 1-2 g / L potassium dihydrogen phosphate, and 0.5-1 g / L magnesium sulfate. The suitable temperature is 30-37° C. and the rotation speed is 150-200 rpm.

[0026] Preferably, in step S11, the material of the ordinary V-shaped mixer is 304 stainless steel that meets food hygiene standards, the stirring speed during mixing is 80-120 rpm, the mixing time is 40-50 minutes, and samples are taken every 10-15 minutes during the mixing process to detect mixing uniformity.

[0027] The present invention has the following beneficial effects:

[0028] 1. The laying hen premix feed additive of the present invention comprises various ingredients such as allicin, yeast cell wall polysaccharide, kelp powder, astragalus extract and the like. Allicin has antibacterial and anti-inflammatory effects, can reduce the number of harmful bacteria in the intestinal tract of laying hens, and maintain intestinal health. Yeast cell wall polysaccharide can enhance the immunity of laying hens and improve their resistance to diseases. Kelp powder is rich in various minerals and trace elements, and provides rich nutrition for laying hens. Astragalus extract can regulate the physiological functions of laying hens and improve the overall health level. Various ingredients work synergistically to achieve nutritional balance and meet the nutritional needs of laying hens at various stages of growth and egg laying.

[0029] 2. During the preparation process, the preparation technology of each component has been carefully designed. For example, the method of water distillation combined with organic solvent extraction can effectively extract high-purity allicin, and ether is selected as the organic solvent to ensure the full extraction of allicin under specific proportions and extraction times; yeast cell wall polysaccharides are made by controlling the fermentation conditions, including temperature, pH value and culture medium composition, so that the yeast strain produces high polysaccharides, thereby improving product quality and output; kelp powder is dried at low temperature and ground in multiple stages to retain the nutrients of kelp to the greatest extent, while the particle size is precisely controlled to facilitate digestion and absorption by laying hens.

[0030] 3. Ingredients such as zinc methionine and selenium powder can participate in various physiological metabolic processes in laying hens. Zinc methionine helps to improve the absorption and utilization of zinc in laying hens, promote the growth and development of laying hens, and improve the quality of eggs. Selenium powder, as an important antioxidant, can improve the reproductive performance and egg production quality of laying hens, reduce the occurrence of soft-shell eggs and broken eggs, and the probiotic metabolites and plant essential oil complexes can also regulate the intestinal microecological balance of laying hens and improve feed utilization, thereby increasing the egg production and egg quality of laying hens. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a graph showing the average egg production of laying hens in the control group, experimental group 1, experimental group 2 and experimental group 3 within 90 days;

[0032] Figure 2 This is a graph showing the changes in average egg weight of laying hens in the control group, experimental group 1, experimental group 2 and experimental group 3 within 90 days;

[0033] Figure 3 This is a graph showing the change in eggshell thickness data for laying hens in the control group, experimental group 1, experimental group 2 and experimental group 3 within 90 days;

[0034] Figure 4 This is a graph showing the change in egg yolk color data for laying hens in the control group, experimental group 1, experimental group 2 and experimental group 3 within 90 days;

[0035] Figure 5 This is a graph showing the changes in the incidence rate of laying hens in the control group, experimental group 1, experimental group 2 and experimental group 3 within 90 days. DETAILED DESCRIPTION

[0036] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0037] The premixed feed additive for laying hens comprises the following raw materials in weight components:

[0038] 10-20 parts of allicin, 30-50 parts of yeast cell wall polysaccharide, 50-80 parts of kelp powder, 20-40 parts of astragalus extract, 10-30 parts of humic acid, 5-10 parts of zinc methionine, 2-8 parts of selenium powder, 5-10 parts of probiotic metabolites, 2-5 parts of plant essential oil complex.

[0039] A method for preparing a premixed feed additive for laying hens, comprising the following preparation process:

[0040] S1, preparing allicin, chopping fresh garlic, putting it into a distillation apparatus, adding water to carry out water distillation, collecting the distillate, and then extracting the distillate with an organic solvent to obtain an organic phase containing allicin;

[0041] S2, preparing yeast cell wall polysaccharides, selecting yeast strains with high polysaccharide production, inoculating them into a glucose liquid medium, wherein the glucose concentration in the glucose liquid medium is 10-15 g / L, the yeast powder concentration is 5-8 g / L, and the peptone concentration is 5-8 g / L, placing them into a conventional fermentation tank, and performing deep fermentation culture at 28-30° C. and pH 5.5-6.5 for 48-72 hours. After the fermentation is completed, the fermentation broth is centrifuged, the precipitate is collected, and an ammonium sulfate solution is added to the precipitate for salting out to precipitate the polysaccharide, and then the precipitate is dissolved in an appropriate amount of buffer, and dialyzed through a dialysis bag to remove salt;

[0042] S3, preparing kelp powder, washing fresh kelp, removing surface impurities, putting it into a low-temperature dryer, and drying it at 40-50°C until the moisture content is less than 10%. Then use a mechanical grinding device to grind the dried kelp into coarse powder, and then put the coarse kelp powder into a ball mill or other grinding and refining equipment for further crushing. When the coarse kelp powder is further crushed in the ball mill or other grinding and refining equipment, the grinding time is 30-60 minutes, and the particle size of the kelp powder reaches 100-200 mesh by adjusting the grinding time and the grinding medium ratio;

[0043] S4, preparing an astragalus extract, crushing the astragalus into particles of appropriate size, putting them into a round-bottom flask, adding an appropriate amount of ethanol, the mass ratio of ethanol to astragalus being 8:1, connecting a reflux condenser, reflux extraction at 70-80° C. for 2-3 times, each extraction for 1-2 hours, and extracting the solution, removing ethanol by vacuum distillation to obtain an astragalus extract;

[0044] S5, preparing humic acid, putting humic acid into a reaction container, adding an oxidant, and determining under acidic or alkaline conditions, controlling the temperature at 60-80°C, stirring the reaction for 3-4 hours according to the type of the selected oxidant, and performing chemical oxidation surface modification on the humic acid. When hydrogen peroxide is used as the oxidant, the mass ratio of humic acid to hydrogen peroxide is 1:0.5-1:1, and the pH value is controlled at 3-4 under acidic conditions; when potassium permanganate is used as the oxidant, the mass ratio of humic acid to potassium permanganate is 1:0.3-1:0.5, and the pH value is controlled at 9-10 under alkaline conditions to optimize the surface modification effect of humic acid. After the reaction is completed, dissolving the modified humic acid in an alkaline aqueous solution, slowly adding an electrolyte solution, and under stirring conditions, allowing the humic acid to reconstruct its structure through solution self-assembly to form an active micro-region;

[0045] S6, preparing methionine, dissolving methionine and an appropriate amount of zinc salt in an appropriate amount of water, and slowly adding the zinc salt solution dropwise to the methionine solution under stirring, while adjusting the pH value of the reaction system to maintain it at 6-7, controlling the reaction temperature at 40-50° C., reacting for 3-4 hours, and filtering, washing, and drying the product after the reaction is completed;

[0046] S7, preparing selenium powder, in a reaction container, dissolving sodium selenite in an appropriate amount of water, adding an appropriate amount of reducing agent, controlling the reaction temperature at 30-40° C. under stirring conditions, reacting for 1-2 hours, so that the sodium selenite is reduced to selenium powder;

[0047] S8, preparing probiotic metabolites, screening specific probiotic strains, inoculating them into a liquid culture medium containing specific nutrients, the specific nutrients include glucose 10-15g / L, peptone 5-8g / L, yeast powder 5-8g / L, potassium dihydrogen phosphate 1-2g / L, magnesium sulfate 0.5-1g / L, the suitable temperature is 30-37°C, the rotation speed is 150-200 rpm, putting them into a shake flask, and fermenting them under suitable temperature and rotation speed conditions for 24-48 hours. After the fermentation is completed, the fermentation broth is centrifuged and the supernatant is collected;

[0048] S9, preparing a plant essential oil complex, selecting thyme essential oil and oregano essential oil, mixing them with the wall material solution in a certain proportion, forming an emulsion under high-speed stirring, and passing the emulsion through a spray drying device, spray drying under the conditions of an inlet air temperature of 150-180° C. and an outlet air temperature of 80-100° C., to prepare a plant essential oil complex;

[0049] S10, special treatment, putting all the pre-treated raw materials into a plasma treatment device, passing nitrogen gas at 50-80°C and 10-20 Pa, and treating for 5-10 minutes;

[0050] S11. Mix thoroughly. Put the raw materials weighed in proportion into an ordinary V-shaped mixer for thorough mixing. The material of the ordinary V-shaped mixer is 304 stainless steel that meets food hygiene standards. The stirring speed during mixing is 80-120 rpm, and the mixing time is 40-50 minutes. During the mixing process, samples are taken every 10-15 minutes to detect the mixing uniformity.

[0051] Embodiment 1

[0052] 100 laying hens with similar health conditions and the same age were selected as the control group. Each time the feed was fed, the laying hens in the control group were fed with feed supplemented with conventional laying hens premix feed additives on the market.

[0053] The breeding cycle is 3 months. The egg production, egg quality (including egg weight, egg shell thickness, yolk color) and morbidity of the laying hens in the control group are recorded every 15 days. The test data are recorded and shown in the following table:

[0054] Table 1: Statistics of various data records during the breeding cycle of the control group

[0055]

[0056] Embodiment 2

[0057] 100 laying hens with similar health conditions and the same age were selected and recorded as experimental group 1. Each time the feed was fed, the laying hens in experimental group 1 were fed with a feed with a laying hen premix feed additive formula of the present invention, wherein each component took an intermediate value, and each component of the additive was prepared by the preparation method of the present invention, namely, 15g of allicin, 40g of yeast cell wall polysaccharide, 65g of kelp powder, 30g of astragalus extract, 20g of humic acid, 7.5g of zinc methionine, 5g of selenium powder, 7.5g of probiotic metabolites, and 3.5g of plant essential oil complex;

[0058] The breeding cycle was 3 months. The egg production, egg quality (including egg weight, egg shell thickness, yolk color) and morbidity of the laying hens in the experimental group 1 were recorded every 15 days. The test data were recorded and the obtained data are shown in the following table:

[0059] Table 2: Statistical table of various data records during the breeding cycle of experimental group 1

[0060]

[0061] Embodiment 3

[0062] 100 laying hens with similar health conditions and the same age were selected and recorded as experimental group 2. Each time the feed was fed, the laying hens in experimental group 2 were fed with a feed containing a laying hen premix feed additive formula with a changed ratio of the ingredients of the present invention. The ingredients of the additive were still prepared by the preparation method of the present invention, and 10 g of allicin, 30 g of yeast cell wall polysaccharide, 50 g of kelp powder, 20 g of astragalus extract, 10 g of humic acid, 5 g of zinc methionine, 2 g of selenium powder, 5 g of probiotic metabolites, and 2 g of plant essential oil complex were taken;

[0063] The breeding cycle was 3 months. The egg production, egg quality (including egg weight, egg shell thickness, yolk color) and morbidity of the laying hens in the experimental group 2 were recorded every 15 days. The test data were recorded and the obtained data are shown in the following table:

[0064] Table 3: Statistical table of various data records during the breeding cycle of experimental group 2

[0065]

[0066] Embodiment 4

[0067] 100 laying hens with similar health conditions and the same age were selected and recorded as experimental group 3. Each time the feed was fed, the laying hens in experimental group 1 were fed with a feed with a laying hen premix feed additive formula of the present invention, wherein each component took an intermediate value, i.e., 15 g of allicin, 40 g of yeast cell wall polysaccharide, 65 g of kelp powder, 30 g of astragalus extract, 20 g of humic acid, 7.5 g of zinc methionine, 5 g of selenium powder, 7.5 g of probiotic metabolites, and 3.5 g of plant essential oil complex; when preparing the additive, allicin was prepared by a method of non-aqueous distillation combined with organic solvent extraction, yeast cell wall polysaccharide was not fermented under controlled conditions, and kelp powder was not subjected to low-temperature drying and multi-stage grinding;

[0068] The breeding cycle was 3 months. The egg production, egg quality (including egg weight, egg shell thickness, yolk color) and morbidity of the laying hens in the experimental group 3 were recorded every 15 days. The test data were recorded and the obtained data are shown in the following table:

[0069] Table 4: Statistical table of various data records during the breeding cycle of experimental group 3

[0070]

[0071] Comprehensively compare the statistical data of Example 1, Example 2, Example 3 and Example 4, and draw the same item data of the four examples into a line graph to obtain Figure 1-5 , according to the data record statistics table of each embodiment and Figure 1-5 Analysis shows that:

[0072] Comparing the control group (Example 1) and experimental group 1 (Example 2), the difference is very significant. Experimental group 1 uses the additive of the standard formula and preparation process of the present invention, and shows a full range of advantages within a breeding cycle of 3 months. From the perspective of egg production, the egg production of the control group for 90 days is 42 eggs / each, while the experimental group 1 is increased to 47 eggs / each, which means that in the same breeding time, each laying hen lays 5 more eggs, which can be calculated according to the number of large-scale breeding, and can greatly increase the output of eggs, bringing higher economic benefits to farmers. In terms of egg quality, the average egg weight increased from 59g in the control group to 63g in the experimental group 1, which not only reflects the optimization of the nutritional intake of laying hens, but also makes eggs more competitive in the market, because heavier eggs are often more popular with consumers. The eggshell thickness increased from 0.31mm to 0.36mm, and thicker eggshells can effectively reduce the breakage rate during transportation and storage, reducing losses. The Roche color fan value of the yolk color increased from 8.2 to 9.5. Egg yolks with brighter colors are generally considered by consumers to have higher nutritional value, which further increases the market value of eggs. At the same time, the incidence rate decreased from 14% to 7%, which shows that the additive of the present invention effectively enhances the immunity of laying hens, reduces the occurrence of diseases, reduces medical costs in the breeding process and the loss of laying hens due to diseases. These data fully demonstrate that the premixed feed additive for laying hens of the present invention can improve the production performance of laying hens in multiple dimensions, improve egg quality, enhance the immunity of laying hens, and reduce the incidence rate.

[0073] Experimental Group 2 (Example 3) changed the proportion of ingredients of the present invention. Although a certain degree of improvement was achieved in terms of egg production and egg quality, the egg production reached 43 eggs per egg in 90 days, and the average egg weight was 61g, but compared with Experimental Group 1, the improvement of various indicators was significantly smaller. For example, the egg production was 4 less than that of Experimental Group 1, and the average egg weight was 2g lower. Moreover, the incidence rate was relatively high, reaching 12%. The reason behind this is that there is a complex synergistic effect between the various ingredients in the feed additives, and each ingredient has its unique function, such as the antibacterial and anti-inflammatory effects of allicin, the immune enhancement of yeast cell wall polysaccharides, and the nutritional supplementation of kelp powder. When the proportions of the ingredients are precisely adjusted, these ingredients can cooperate with each other to exert the greatest effect. However, once the ratio changes, the synergistic effect between the ingredients will be affected, and they will not be able to fully exert their respective roles, which will greatly reduce the effect of the additive on the growth, egg-laying performance and egg quality of laying hens. Therefore, the precise formulation of the ingredient ratio proposed in the present invention is crucial to the effect of the additive. The appropriate ingredient ratio can better exert the synergistic effect of the ingredients, thereby more effectively promoting the growth of laying hens, improving egg-laying performance and improving egg quality.

[0074] Experimental group 3 (Example 4) adopts the standard component ratio of the present invention, but changes are made in the preparation method. Allicin adopts the method of non-aqueous distillation combined with organic solvent extraction, yeast cell wall polysaccharide does not control the fermentation conditions, and kelp powder does not undergo low-temperature drying and multi-stage grinding. The experimental results show that the improvement of its various indicators is less than that of experimental group 1. The egg production is 45 eggs / each in 90 days, which is 2 less than that of experimental group 1; the average egg weight is 61g, which is lower than 63g of experimental group 1. This is because scientific preparation technology plays an indispensable role in ensuring product quality and giving full play to the efficacy of additives. Taking the preparation of allicin as an example, the method of water distillation combined with specific organic solvent extraction can better retain the active ingredients of allicin, while the method of non-aqueous distillation combined with organic solvent extraction may lead to a decrease in the activity of allicin, thereby affecting its antibacterial and anti-inflammatory effects. The control of the fermentation conditions of yeast cell wall polysaccharides is crucial to their yield and activity. Failure to control the fermentation conditions will reduce the yield and quality of polysaccharides and fail to give full play to their immune enhancement effects. The kelp powder can better retain its nutrients through low-temperature drying and multi-stage grinding, and the particle size is more suitable for digestion and absorption by laying hens. Without these treatments, the nutritional value and utilization rate of the kelp powder will be reduced. Only by strictly following the preparation method proposed by the present invention can the activity and characteristics of each component be effectively retained, thereby achieving a significant improvement in the production performance of laying hens.

[0075] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A premixed feed additive for laying hens, characterized in that: The raw material composition includes the following components by weight: 10-20 parts of allicin, 30-50 parts of yeast cell wall polysaccharide, 50-80 parts of kelp powder, 20-40 parts of astragalus extract, 10-30 parts of humic acid, 5-10 parts of zinc methionine, 2-8 parts of selenium powder, 5-10 parts of probiotic metabolites, 2-5 parts of plant essential oil complex.

2. The method for preparing a premixed feed additive for laying hens according to claim 1, characterized in that: The preparation process includes the following: S1, preparing allicin, chopping fresh garlic, putting it into a distillation apparatus, adding water to carry out water distillation, collecting the distillate, and then extracting the distillate with an organic solvent to obtain an organic phase containing allicin; S2, preparing yeast cell wall polysaccharides, selecting yeast strains with high polysaccharide production, inoculating them into glucose liquid culture medium, placing them into a conventional fermentation tank, and carrying out deep fermentation culture for 48-72 hours at 28-30°C and pH 5.5-6.

5. After the fermentation is completed, centrifuging the fermentation broth, collecting the precipitate, adding ammonium sulfate solution to the precipitate for salting out to precipitate the polysaccharide, and then dissolving the precipitate in an appropriate amount of buffer solution, and dialyzing through a dialysis bag to remove salt; S3, preparing kelp powder, washing fresh kelp, removing surface impurities, placing in a low-temperature dryer, drying at 40-50° C. until the moisture content is less than 10%, then grinding the dried kelp into coarse powder using a mechanical grinding device, and then placing the coarse kelp powder into a ball mill or other grinding and refining equipment for further crushing; S4, preparing an astragalus extract, crushing the astragalus into particles of appropriate size, putting them into a round-bottom flask, adding an appropriate amount of ethanol, the mass ratio of ethanol to astragalus being 8:1, connecting a reflux condenser, reflux extraction at 70-80° C. for 2-3 times, each extraction for 1-2 hours, and extracting the solution, removing ethanol by vacuum distillation to obtain an astragalus extract; S5, preparing humic acid, putting humic acid into a reaction container, adding an oxidant, and controlling the temperature at 60-80° C. under acidic or alkaline conditions according to the type of the selected oxidant, stirring the reaction for 3-4 hours, and performing chemical oxidation on the surface of the humic acid. After the reaction is completed, dissolving the modified humic acid in an alkaline aqueous solution, slowly adding an electrolyte solution, and under stirring conditions, allowing the humic acid to reconstruct its structure through solution self-assembly to form an active micro-region; S6, preparing methionine, dissolving methionine and an appropriate amount of zinc salt in an appropriate amount of water, and slowly adding the zinc salt solution dropwise to the methionine solution under stirring, while adjusting the pH value of the reaction system to maintain it at 6-7, controlling the reaction temperature at 40-50° C., reacting for 3-4 hours, and filtering, washing, and drying the product after the reaction is completed; S7, preparing selenium powder, in a reaction container, dissolving sodium selenite in an appropriate amount of water, adding an appropriate amount of reducing agent, controlling the reaction temperature at 30-40° C. under stirring conditions, reacting for 1-2 hours, so that the sodium selenite is reduced to selenium powder; S8, preparing probiotic metabolites, screening specific probiotic strains, inoculating them into a liquid culture medium containing specific nutrients, placing them in a shake flask, and fermenting them in the shake flask for 24-48 hours under suitable temperature and rotation speed conditions. After the fermentation is completed, the fermentation liquid is centrifuged and the supernatant is collected; S9, preparing a plant essential oil complex, selecting thyme essential oil and oregano essential oil, mixing them with the wall material solution in a certain proportion, forming an emulsion under high-speed stirring, and passing the emulsion through a spray drying device, spray drying under the conditions of an inlet air temperature of 150-180° C. and an outlet air temperature of 80-100° C., to prepare a plant essential oil complex; S10, special treatment, putting all the pre-treated raw materials into a plasma treatment device, passing nitrogen gas at 50-80°C and 10-20 Pa, and treating for 5-10 minutes; S11, fully mixing: put the raw materials weighed in proportion into a common V-type mixer for full mixing.

3. The method for preparing a premixed feed additive for laying hens according to claim 2, characterized in that: In step S1, the organic solvent is diethyl ether, and during the extraction process, the volume ratio of diethyl ether to distillate is 1:2-1:3, and the number of extractions is 2-3 times.

4. The method for preparing a premixed feed additive for laying hens according to claim 2, characterized in that: In step S2, the glucose concentration in the glucose liquid culture medium is 10-15 g / L, the yeast powder concentration is 5-8 g / L, and the peptone concentration is 5-8 g / L.

5. The method for preparing a premixed feed additive for laying hens according to claim 2, characterized in that: In step S3, when the kelp coarse powder is further crushed in a ball mill or other grinding and refining equipment, the grinding time is 30-60 minutes, and the particle size of the kelp powder reaches 100-200 mesh by adjusting the grinding time and the grinding medium ratio.

6. The method for preparing a premixed feed additive for laying hens according to claim 2, characterized in that: In the step S5, when hydrogen peroxide is used as the oxidant, the mass ratio of humic acid to hydrogen peroxide is 1:0.5-1:1, and the pH value is controlled at 3-4 under acidic conditions; when potassium permanganate is used as the oxidant, the mass ratio of humic acid to potassium permanganate is 1:0.3-1:0.5, and the pH value is controlled at 9-10 under alkaline conditions to optimize the surface modification effect of humic acid.

7. The method for preparing a premixed feed additive for laying hens according to claim 2, characterized in that: In step S8, the specific nutrients include 10-15 g / L glucose, 5-8 g / L peptone, 5-8 g / L yeast powder, 1-2 g / L potassium dihydrogen phosphate, and 0.5-1 g / L magnesium sulfate. The suitable temperature is 30-37° C. and the rotation speed is 150-200 rpm.

8. The method for preparing a premixed feed additive for laying hens according to claim 2, characterized in that: In step S11, the material of the ordinary V-shaped mixer is 304 stainless steel that meets the food hygiene standards, the stirring speed during mixing is 80-120 rpm, the mixing time is 40-50 minutes, and samples are taken every 10-15 minutes during the mixing process to detect the mixing uniformity.

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