Functional feed for increasing laying rate of poultry and preparation method of functional feed

Abstract

The invention discloses a functional feed for increasing the laying rate of poultry and a preparation method of the functional feed, and particularly relates to the technical field of poultry feeds, the functional feed comprises the following components by content: 0.6%-0.8% of algal polysaccharides, 1.2%-1.5% of an amino acid compound, 0.3%-0.4% of a rosemary extract, 0.15%-0.25% of a desert factor, and the balance of conventional poultry feed raw materials. The preparation method specifically comprises the following steps: S1, superfine grinding; S2, nanometer wrapping; S3, low-temperature dynamic mixing; and S4, biological fermentation regulation. The feed disclosed by the invention can accurately regulate and control the reproductive system of poultry, promote follicle development, optimize protein synthesis, remarkably improve and stabilize the laying rate, enhance the quality of eggshells, enhance the immunity of the poultry, improve the environmental adaptability of the poultry, reduce the occurrence of diseases and improve the quality and efficacy of the feed.

Description

Technical Field

[0001] The present invention relates to the technical field of poultry feed, and more specifically, to a functional feed for improving the egg production rate of poultry and a preparation method thereof. Background Art

[0002] In the poultry farming industry, egg production rate and egg quality are the core indicators for measuring economic benefits and product quality. With the global population growth and the continuous increase in people's demand for poultry eggs, how to improve poultry egg production performance has become the focus of the industry.

[0003] Traditional poultry farming relies on conventional feed formulas, which are mainly composed of energy feed (such as corn, wheat, etc.), protein feed (such as soybean meal, fish meal), mineral feed and a small amount of additives. Although this type of feed can meet the basic growth and maintenance needs of poultry, it has obvious limitations in improving egg production rates, such as:

[0004] 1. From the perspective of nutritional ingredients, conventional feed lacks special ingredients that can accurately stimulate the reproductive system of poultry and promote follicle development. The poultry reproductive hormone secretion and regulation system is complex, and it is difficult to achieve efficient regulation by conventional nutrients alone, resulting in delayed follicle development or poor quality, which in turn limits the increase in egg production rate;

[0005] 2. In terms of egg quality, existing feeds are difficult to ensure balanced nutrition and high-quality eggshell formation. The amino acid composition in conventional feeds cannot fully meet the needs of poultry for efficient protein synthesis, resulting in low Haugh units of eggs, and difficulty in improving freshness and protein quality. At the same time, the supply of mineral feeds is insufficient in terms of calcium-phosphorus ratio and absorption utilization, resulting in thin and fragile eggshells that are easily damaged during transportation and storage, reducing the commercial value of eggs.

[0006] 3. Poultry health protection also faces challenges. With the increase in breeding density and environmental changes, poultry are susceptible to pathogens and their immunity decreases. Conventional feed lacks effective immune-enhancing ingredients. Poultry have weak resistance to diseases and the incidence rate increases, which not only affects egg production performance but also increases breeding costs. In addition, poultry have poor adaptability to environmental stress. Environmental factors such as high temperature, cold, and humidity changes can easily trigger physiological stress responses in poultry, interfere with normal reproductive physiological processes, and lead to fluctuations in egg production rates.

[0007] 4. In the feed preparation process, traditional methods are insufficient in protecting and utilizing nutrients. Ordinary crushing technology cannot fully refine grain raw materials, and the digestion and absorption rate of poultry is limited. There is a lack of effective protection measures for easily oxidized and unstable nutrients, and they are easily lost in activity during processing and storage. The mixing process cannot ensure the uniform distribution of various ingredients, affecting the consistency of feed quality. The fermentation process lacks specificity and cannot effectively promote nutrient conversion and regulate the intestinal microecology of poultry.

[0008] In view of the above situation, the present invention provides a functional feed for improving the egg production rate of poultry and a preparation method thereof. Summary of the invention

[0009] In order to overcome the above-mentioned defects of the prior art, the present invention provides a functional feed for improving the egg production rate of poultry and a preparation method thereof, so as to solve the problems raised in the above-mentioned background technology.

[0010] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a functional feed for improving the egg production rate of poultry, comprising the following ingredients and contents: algae polysaccharide 0.6%-0.8%, amino acid complex 1.2%-1.5%, rosemary extract 0.3%-0.4%, desert factor 0.15%-0.25%, and the rest are conventional poultry feed raw materials.

[0011] Preferably, the algae polysaccharide is specifically extracted from deep-sea red algae species under the conditions of enzyme concentration of 0.5%-1.0%, enzymolysis temperature of 40-45°C, and enzymolysis time of 2-3h, and then separated and purified by ultrafiltration technology using an ultrafiltration membrane with a molecular weight cutoff of 5000-10000Da, with a purity of more than 95%.

[0012] Preferably, the amino acid complex is an amino acid complex composed of arginine, glycine and phytaminophen extracted from Rhodiola rosea in a ratio of 3:2:1, wherein the purity of arginine is 98% and the purity of glycine is 99%;

[0013] The plant amino acid is extracted by ethanol reflux extraction, with an ethanol concentration of 70%-80%, a reflux temperature of 75-85°C, and an extraction time of 3-4h. After extraction, it is separated and purified by column chromatography with a purity of more than 90%. Finally, it is prepared by liquid phase synthesis.

[0014] Preferably, the rosemary extract is an active ingredient extracted from rosemary, using supercritical carbon dioxide extraction technology, extraction pressure 20-25MPa, extraction temperature 40-45°C, carbon dioxide flow rate 20-30L / h, extraction time 2-3h, and the obtained extract has a carnosic acid content of 30%-35% and a rosmarinic acid content of 25%-30%.

[0015] Preferably, the desert factor is separated from the rhizosphere microbial community of the desert plant seabuckthorn, and the microorganisms are collected by centrifugation at a speed of 8000-10000r / min and a centrifugation time of 10-15min. Then, the salting-out method is used, and the ammonium sulfate saturation is controlled at 60%-70%. It is then purified by gel filtration chromatography to a purity of more than 85%.

[0016] Preferably, the conventional poultry feed raw materials include but are not limited to energy feed, protein feed, mineral feed, vitamin premix and additives;

[0017] Energy feed is specifically corn and wheat, accounting for 45%-55% of the balance, of which the moisture content of corn is controlled at 13%-15%, the bulk density is not less than 700g / L, the moisture content of wheat is 12%-14%, and the imperfect kernels do not exceed 6%;

[0018] The protein feed is specifically soybean meal and fish meal, accounting for 22%-32% of the balance, wherein the crude protein content of soybean meal is not less than 43%, the urease activity is between 0.05-0.3U / g, the crude protein content of fish meal is 60%-65%, and the salt content does not exceed 2%;

[0019] The mineral feed specifically includes stone powder, shell powder and calcium hydrogen phosphate, accounting for 6%-9% of the balance, among which the calcium content of stone powder is 38%-40%, the calcium content of shell powder is 36%-38%, the calcium content of calcium hydrogen phosphate is 21%-23%, and the phosphorus content is 16%-18%;

[0020] The vitamin premix specifically comprises vitamins A, D, E and B vitamins, accounting for 1.5%-2.5% of the balance, wherein the content of vitamin A is 5000-8000 IU / kg, the content of vitamin D is 500-1000 IU / kg, and the content of vitamin E is 50-100 mg / kg;

[0021] B vitamins include vitamin B1, B2, B6, B12, niacin, pantothenic acid, folic acid and biotin. Among them, the content of vitamin B1 is 10-15 mg / kg, the content of vitamin B2 is 15-20 mg / kg, the content of vitamin B6 is 8-12 mg / kg, the content of vitamin B12 is 0.01-0.02 mg / kg, the content of niacin is 30-40 mg / kg, the content of pantothenic acid is 15-25 mg / kg, the content of folic acid is 2-3 mg / kg, and the content of biotin is 0.1-0.2 mg / kg.

[0022] The additives specifically include mildew preventers, antioxidants and flavoring agents, which account for 0.3%-0.8% of the balance. Among them, the mildew preventer is propionic acid, the content of which is not less than 99%, and the added amount is 0.1%-0.3%. The antioxidant is ethoxyquinoline, the content of which is 95%-98%, and the added amount is 0.01%-0.02%. The flavoring agent is ethyl lactate, and the added amount is 0.05%-0.1%.

[0023] A preparation method for preparing the above-mentioned functional feed for improving poultry egg production rate, specifically comprising the following steps:

[0024] S1, ultrafine grinding, the corn and wheat are ultrafinely ground by air jet mill ultrafine grinding equipment, the particle size is reduced to 6-8μm under the conditions of pressure 0.8-1.0MPa and feed rate 5-8kg / h, and the speed of the classifying wheel of the equipment is 3000-4000r / min;

[0025] S2, nano-encapsulation, using nano-encapsulation technology to encapsulate algae polysaccharide and rosemary extract using edible nano-scale liposome materials, and the liposome particle size is controlled at 80-150 nanometers;

[0026] S3, low temperature dynamic mixing, using a jacketed horizontal mixer to perform low temperature dynamic mixing on the processed raw materials, low temperature circulating water is introduced into the jacket, the mixing temperature is controlled at 12-13°C, the speed is set at 150-200r / min, the mixing time is 20-30min, and the filling factor of the mixer is controlled between 0.6-0.7;

[0027] S4, biological fermentation regulation, the egg-laying promoting bacteria were introduced for biological fermentation regulation, and the concentration of inoculation in the mixed feed was 1×10 8 -5×10 8 CFU / g of bacterial liquid, fermented at 26-28℃ for 36-42h, during the fermentation process, the content of acetic acid, propionic acid and butyric acid increased by 32%-38%, the pH value was maintained between 6.2-6.4, and the crude protein digestibility of feed increased by 9%-10% after fermentation

[0028] Preferably, in step S2, the edible nano-liposome material uses soybean lecithin as the main raw material, the soybean lecithin is dissolved in a chloroform organic solvent, the solvent is removed by rotary evaporation at 43-47°C to form a film, and then an aqueous solution containing the components to be encapsulated formed by dissolving or dispersing algae polysaccharide and rosemary extract in deionized water is added, and ultrasonic treatment is performed at an ultrasonic power of 220-280 W for 12-14 minutes, and the encapsulation rate reaches 82%-84%.

[0029] Preferably, in step S4, the egg-laying promoting bacteria is Bacillus subtilis in the poultry intestine.

[0030] Technical effects and advantages of the present invention:

[0031] 1. The present invention incorporates algae polysaccharide into the feed formula. Its unique molecular structure can stimulate the reproductive hormone secretion regulation system in poultry, promote the development and maturation of follicles, and provide a good physiological basis for egg production. It is combined with an amino acid complex composed of a specific proportion to optimize the protein synthesis pathway, enhance the liver's metabolic capacity, and provide sufficient energy and material support for egg production. The synergistic effect of the two can effectively improve the ovulation frequency and quality of poultry, thereby significantly improving the egg production rate;

[0032] At the same time, the addition of rosemary extract and desert factor further optimizes the reproductive environment and body adaptability of poultry. The antioxidant and phenolic compounds in rosemary extract improve the microenvironment of the reproductive system, and the desert factor regulates the water balance in the body, enhances the adaptability of poultry to environmental stress, reduces egg production fluctuations caused by environmental changes, and ensures a stable increase in egg production rate.

[0033] 2. The present invention helps the efficient synthesis of protein in poultry through the reasonable ratio of amino acid complexes, which is reflected in the eggs and can increase the Haugh unit, indicating that the freshness and protein quality of the eggs are significantly improved. At the same time, the rich and balanced nutrients in the feed prompt the poultry to inject more nutrients into the eggs during the egg-laying process, thereby improving the nutritional value of the eggs;

[0034] At the same time, the stone powder, shell powder and calcium hydrogen phosphate in the mineral feed provide poultry with sufficient calcium, phosphorus and other mineral elements, which help poultry form eggshells with tight structure and high strength, increase the thickness of eggshells, reduce the breakage rate of eggs during transportation and storage, and improve the commercial value of eggs;

[0035] 3. The rosemary extract of the present invention has a variety of antioxidants and phenolic compounds, which can enhance the immunity of poultry and improve its ability to resist pathogen invasion. By regulating the immune system of poultry, the probability of disease occurrence is reduced, the health of poultry is guaranteed, and continuous and stable egg production is guaranteed;

[0036] At the same time, the desert factor starts from regulating the water balance in the poultry body, enhancing its adaptability to environmental stress. Whether it is a harsh environment such as high temperature and drought, or daily changes in the breeding environment, poultry can better adapt, reduce physiological stress reactions caused by environmental factors, reduce morbidity, and maintain good production performance;

[0037] 4. In terms of preparation method, the present invention uses ultrafine grinding technology to reduce the particle size of grain raw materials to 6-8 μm, which greatly increases the surface area of ​​the raw materials and improves the digestion and absorption rate of nutrients by poultry. The nano-encapsulation technology uses edible nano-level liposome materials to encapsulate the easily oxidized and unstable algae polysaccharides, rosemary extracts and other ingredients, and controls the liposome particle size to 80-150 nanometers, which not only protects these ingredients from oxidation and degradation, but also achieves slow release in the intestine, thereby improving bioavailability.

[0038] At the same time, low-temperature dynamic mixing technology, in a low-temperature environment of 12-13°C, reasonably controls the stirring speed and time to evenly mix various raw materials, avoiding the destruction of heat-sensitive nutrients by high temperature. The biological fermentation regulation technology introduces egg-laying promoting bacteria, which ferment under suitable temperature and time conditions. The egg-laying promoting bacteria decomposes large-molecule nutrients and produces beneficial metabolites such as short-chain fatty acids, further improving the nutritional value and palatability of the feed. At the same time, it regulates the balance of poultry intestinal microecology, promotes the absorption and utilization of nutrients, and comprehensively guarantees the quality and efficacy of the feed. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 It is the overall flow chart of the present invention. DETAILED DESCRIPTION

[0040] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Embodiment 1,

[0042] This embodiment provides a functional feed for improving the egg production rate of poultry, which specifically includes the following ingredients and contents:

[0043] Algae polysaccharide, raw materials are collected from deep-sea red algae species, and a composite enzyme of cellulase and pectinase (mass ratio 2:1) is used, the enzyme concentration is 0.7%, and enzymatic hydrolysis is carried out at 43°C for 2.5 hours, and then ultrafiltration purification is carried out using an ultrafiltration membrane with a molecular weight cutoff of 6000Da, and finally algae polysaccharide with a purity of 96% is obtained for standby use;

[0044] An amino acid complex is prepared by extracting phytaminophen from Rhodiola rosea by using arginine with a purity of 98% and glycine with a purity of 99%. The ethanol reflux extraction method is used, with an ethanol concentration of 75%, a reflux temperature of 80°C, and an extraction time of 3.5 hours. After extraction, the amino acid complex is separated and purified by column chromatography, and the purity reaches 91%. The amino acid complex is prepared by a liquid phase synthesis method according to a ratio of arginine, glycine, and phytaminophen of 3:2:1, and is set aside.

[0045] Rosemary extract, collect rosemary, use supercritical carbon dioxide extraction technology, extraction pressure 23MPa, extraction temperature 43°C, carbon dioxide flow rate 23L / h, extraction time 2.5h, the obtained extract has a carnosic acid content of 33% and a rosmarinic acid content of 28%, and is reserved;

[0046] Desert factor: collect rhizosphere soil samples of seabuckthorn, centrifuge at 9000r / min for 12min to collect microorganisms, use ammonium sulfate precipitation method, control the ammonium sulfate saturation at 65%, and then purify by dextran gel G-50 chromatography to obtain desert factor with a purity of 86%, which is set aside;

[0047] Conventional poultry feed ingredients:

[0048] Energy feed, corn, moisture content 14%, bulk density 730g / L, wheat, moisture content 13%, imperfect grains 5%;

[0049] Protein feed, selected soybean meal, crude protein content 44%, urease activity 0.15U / g, white fish meal, crude protein content 63%, salt 1.2%;

[0050] Mineral feed, calcite powder, calcium content 39%, shell powder, calcium content 37%, thermally produced calcium hydrogen phosphate, calcium content 22%, phosphorus content 17%;

[0051] Vitamin premix, prepared according to the following contents: vitamin A 6500 IU / kg, vitamin D 700 IU / kg, vitamin E 70 mg / kg, vitamin B1 12 mg / kg, vitamin B2 18 mg / kg, vitamin B6 10 mg / kg, vitamin B12 0.015 mg / kg, niacin 35 mg / kg, pantothenic acid 20 mg / kg, folic acid 2.5 mg / kg and biotin 0.15 mg / kg;

[0052] As additives, a mildew preventer with a propionic acid content of 99.5%, an antioxidant with an ethoxyquinoline content of 96%, and ethyl lactate are selected as a flavoring agent.

[0053] The embodiment of the present invention also provides a preparation method for preparing the above-mentioned functional feed for improving the egg production rate of poultry, which specifically comprises the following steps:

[0054] S1, ultrafine grinding, corn and wheat are put into the air jet mill ultrafine grinding equipment, and ultrafine grinding is carried out under the conditions of pressure 0.9MPa, feed rate 6kg / h, and the speed of the classifying wheel of the equipment is 3500r / min, so that the particle size is reduced to 7μm;

[0055] S2, nano-encapsulation, preparing nano-sized liposomes with soybean lecithin as the main raw material, dissolving soybean lecithin in chloroform, removing the solvent by rotary evaporation at 45°C to form a film, dissolving or dispersing algae polysaccharide and rosemary extract in deionized water to form an aqueous solution containing the components to be encapsulated, adding the aqueous solution to the film, and ultrasonically treating the film at an ultrasonic power of 250W for 13 minutes, so that the liposome particle size is controlled to 100 nanometers, and the encapsulation rate reaches 83%;

[0056] S3, low-temperature dynamic mixing, using a horizontal mixer with a jacket, passing low-temperature circulating water into the jacket, controlling the mixing temperature at 12.5°C, setting the speed to 170r / min, mixing time to 25min, and the mixer filling factor to 0.65, adding ultrafinely crushed energy feed, protein feed, mineral feed, vitamin premix, additives, nano-encapsulated algae polysaccharide and rosemary extract, amino acid complex, and desert factor in sequence, and mixing them evenly;

[0057] S4. Biological fermentation regulation: Bacillus subtilis from poultry intestines was selected as egg-laying promoting bacteria and inoculated into the mixed feed at a concentration of 3×10 8 CFU / g of bacterial liquid was fermented at 27℃ for 38h. During the fermentation process, the contents of acetic acid, propionic acid and butyric acid increased by 35%, the pH value was maintained at 6.3, and the crude protein digestibility of the feed increased by 9.5% after fermentation.

[0058] The functional feed prepared in this example was tested for performance in the following manner:

[0059] 300 laying hens in good health and of uniform age were selected and randomly divided into an experimental group and a control group, with 150 laying hens in each group. The experimental group was fed with the functional feed prepared in this example, and the control group was fed with conventional feed. After an 8-week feeding experiment, relevant data were recorded:

[0060] 1. Egg laying rate: The average egg laying rate of the experimental group reached 92%, which was 12 percentage points higher than 80% of the control group;

[0061] 2. Egg quality: The average Haugh unit of the eggs in the experimental group was 85, higher than 80 in the control group, and the eggshell thickness was 0.38 mm, thicker than 0.35 mm in the control group;

[0062] 3. Poultry health status: The morbidity rate of poultry in the experimental group was 5%, which was lower than 10% in the control group.

[0063] Embodiment 2,

[0064] This embodiment provides a functional feed for improving the egg production rate of poultry, which specifically includes the following ingredients and contents:

[0065] Algae polysaccharide, adjust the enzymatic hydrolysis conditions, enzyme concentration 0.6%, enzymatic hydrolysis temperature 42 ° C, enzymatic hydrolysis time 3h, the remaining extraction and purification steps are the same as Example 1, and finally obtain algae polysaccharide with a purity of 95.5%;

[0066] The amino acid complex, when extracting the plant amino acid, the ethanol concentration is adjusted to 72%, the reflux temperature is 78°C, the extraction time is 4h, and the other steps remain unchanged to prepare the amino acid complex;

[0067] Rosemary extract, the extraction pressure is adjusted to 22MPa, the carbon dioxide flow rate is 22L / h, and the other extraction conditions are the same as in Example 1 to obtain an extract;

[0068] For the desert factor, the centrifugal speed is adjusted to 8500 r / min, the ammonium sulfate saturation is controlled at 62%, and the other purification steps remain unchanged to obtain the desert factor;

[0069] Conventional poultry feed raw materials, the same as in Example 1, but the bulk density of corn is 720 g / L, and the imperfect wheat kernels are 4%;

[0070] The embodiment of the present invention also provides a preparation method for preparing the above-mentioned functional feed for improving the egg production rate of poultry, which specifically comprises the following steps:

[0071] S1, ultrafine grinding, the pressure is adjusted to 0.85MPa, the feed rate is 7kg / h, the speed of the classifying wheel of the equipment is 3300r / min, and the rest is the same as in Example 1;

[0072] S2, nano-wrapping, ultrasonic power adjusted to 230W, ultrasonic treatment time 14min, the rest is the same as Example 1;

[0073] S3, low temperature dynamic mixing, the mixing temperature is controlled at 12°C, the rotation speed is 160r / min, the mixing time is 28min, the mixer filling factor is 0.62, and the rest is the same as in Example 1;

[0074] S4, biological fermentation regulation, egg-laying promoting bacteria access concentration was adjusted to 2×10 8 CFU / g, fermentation temperature 26°C, fermentation time 40h, and other monitoring indicators are the same as those in Example 1.

[0075] The functional feed prepared in this example was tested for performance in the following manner:

[0076] Similarly, 300 laying hens in good health and of the same age were selected and grouped in the same manner as in Example 1. The experimental group was fed with the functional feed prepared in this example, and the control group was fed with conventional feed. After 8 weeks of feeding experiment, the recorded data were as follows:

[0077] 1. Egg laying rate: The average egg laying rate of the experimental group was 90%, which was 10 percentage points higher than that of the control group;

[0078] 2. Egg quality: The average Haugh unit of eggs in the experimental group was 83, and the eggshell thickness was 0.37 mm;

[0079] 3. Poultry health status: the morbidity rate of poultry in the experimental group was 6%.

[0080] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A functional feed for improving the egg production rate of poultry, characterized by: It contains the following ingredients and contents: algae polysaccharide 0.6%-0.8%, amino acid complex 1.2%-1.5%, rosemary extract 0.3%-0.4%, desert factor 0.15%-0.25%, and the rest are conventional poultry feed raw materials.

2. The functional feed for improving poultry egg production rate according to claim 1, characterized in that: The algae polysaccharide is specifically extracted from deep-sea red algae species under the conditions of an enzyme concentration of 0.5%-1.0%, an enzymatic hydrolysis temperature of 40-45°C, and an enzymatic hydrolysis time of 2-3h, and then separated and purified by ultrafiltration technology using an ultrafiltration membrane with a molecular weight cutoff of 5000-10000Da, with a purity of more than 95%.

3. The functional feed for improving poultry egg production rate according to claim 2, characterized in that: The amino acid complex is composed of arginine, glycine and phytogenic acid extracted from Rhodiola rosea in a ratio of 3:2:1, wherein the purity of arginine is 98% and the purity of glycine is 99%; The plant amino acid is extracted by ethanol reflux extraction, with an ethanol concentration of 70%-80%, a reflux temperature of 75-85°C, and an extraction time of 3-4h. After extraction, it is separated and purified by column chromatography with a purity of more than 90%. Finally, it is prepared by liquid phase synthesis.

4. The functional feed for improving poultry egg production rate according to claim 3, characterized in that: The rosemary extract is an active ingredient extracted from rosemary, and adopts supercritical carbon dioxide extraction technology, with an extraction pressure of 20-25MPa, an extraction temperature of 40-45°C, a carbon dioxide flow rate of 20-30L / h, and an extraction time of 2-3h. The obtained extract has a carnosic acid content of 30%-35% and a rosmarinic acid content of 25%-30%.

5. The functional feed for improving poultry egg production rate according to claim 4, characterized in that: The desert factor is separated from the rhizosphere microbial community of the desert plant seabuckthorn. The microorganisms are collected by centrifugation with a centrifugal speed of 8000-10000 r / min and a centrifugal time of 10-15 minutes. Then, the salting-out method is used with the ammonium sulfate saturation controlled at 60%-70%, and then the product is purified by gel filtration chromatography with a purity of more than 85%.

6. The functional feed for improving the egg production rate of poultry according to claim 5, characterized in that: The conventional poultry feed raw materials include but are not limited to energy feed, protein feed, mineral feed, vitamin premix and additives; Energy feed is specifically corn and wheat, accounting for 45%-55% of the balance, of which the moisture content of corn is controlled at 13%-15%, the bulk density is not less than 700g / L, the moisture content of wheat is 12%-14%, and the imperfect kernels do not exceed 6%; The protein feed is specifically soybean meal and fish meal, accounting for 22%-32% of the balance, wherein the crude protein content of soybean meal is not less than 43%, the urease activity is between 0.05-0.3U / g, the crude protein content of fish meal is 60%-65%, and the salt content does not exceed 2%; The mineral feed specifically includes stone powder, shell powder and calcium hydrogen phosphate, accounting for 6%-9% of the balance, among which the calcium content of stone powder is 38%-40%, the calcium content of shell powder is 36%-38%, the calcium content of calcium hydrogen phosphate is 21%-23%, and the phosphorus content is 16%-18%; The vitamin premix specifically comprises vitamins A, D, E and B vitamins, accounting for 1.5%-2.5% of the balance, wherein the content of vitamin A is 5000-8000 IU / kg, the content of vitamin D is 500-1000 IU / kg, and the content of vitamin E is 50-100 mg / kg; B vitamins include vitamin B1, B2, B6, B12, niacin, pantothenic acid, folic acid and biotin. Among them, the content of vitamin B1 is 10-15 mg / kg, the content of vitamin B2 is 15-20 mg / kg, the content of vitamin B6 is 8-12 mg / kg, the content of vitamin B12 is 0.01-0.02 mg / kg, the content of niacin is 30-40 mg / kg, the content of pantothenic acid is 15-25 mg / kg, the content of folic acid is 2-3 mg / kg, and the content of biotin is 0.1-0.2 mg / kg. The additives specifically include mildew preventers, antioxidants and flavoring agents, which account for 0.3%-0.8% of the balance. Among them, the mildew preventer is propionic acid, the content of which is not less than 99%, and the added amount is 0.1%-0.3%. The antioxidant is ethoxyquinoline, the content of which is 95%-98%, and the added amount is 0.01%-0.02%. The flavoring agent is ethyl lactate, and the added amount is 0.05%-0.1%.

7. A preparation method for preparing the functional feed for improving poultry egg production rate according to claim 6, characterized in that: The specific steps include: S1, ultrafine grinding, the corn and wheat are ultrafinely ground by air jet mill ultrafine grinding equipment, the particle size is reduced to 6-8μm under the conditions of pressure 0.8-1.0MPa and feed rate 5-8kg / h, and the speed of the classifying wheel of the equipment is 3000-4000r / min; S2, nano-encapsulation, using nano-encapsulation technology to encapsulate algae polysaccharide and rosemary extract using edible nano-scale liposome materials, and the liposome particle size is controlled at 80-150 nanometers; S3, low temperature dynamic mixing, using a jacketed horizontal mixer to perform low temperature dynamic mixing on the processed raw materials, low temperature circulating water is introduced into the jacket, the mixing temperature is controlled at 12-13°C, the speed is set at 150-200r / min, the mixing time is 20-30min, and the filling factor of the mixer is controlled between 0.6-0.7; S4, biological fermentation regulation, the egg-laying promoting bacteria were introduced for biological fermentation regulation, and the concentration of inoculation in the mixed feed was 1×10 8 -5×10 8 CFU / g of bacterial liquid was fermented at 26-28℃ for 36-42h. During the fermentation process, the contents of acetic acid, propionic acid and butyric acid increased by 32%-38%, and the pH value was maintained between 6.2-6.

4. After fermentation, the crude protein digestibility of feed increased by 9%-10%.

8. The preparation method according to claim 7, characterized in that: In step S2, the edible nano-scale liposome material uses soybean lecithin as the main raw material. The soybean lecithin is dissolved in a chloroform organic solvent, and the solvent is removed by rotary evaporation at 43-47° C. to form a film. Then, an aqueous solution containing the components to be encapsulated formed by dissolving or dispersing algae polysaccharide and rosemary extract in deionized water is added, and ultrasonic treatment is performed at an ultrasonic power of 220-280 W for 12-14 minutes, and the encapsulation rate reaches 82%-84%.

9. The preparation method according to claim 8, characterized in that: In step S4, the egg-laying promoting bacteria are Bacillus subtilis in the poultry intestine.

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