Dendrobium officinale tender stem lozenge as well as preparation method and application thereof

Abstract

The invention relates to the technical field of health care product development, in particular to a dendrobium officinale tender stem lozenge and a preparation method and application thereof. The dendrobium officinale tender stem lozenge is prepared from a dendrobium officinale tender stem composition and pharmaceutically acceptable auxiliary materials, the dendrobium officinale tender stem composition is prepared from the following raw materials in parts by weight: 90 to 120 parts of dendrobium officinale tender stems, 3 to 6 parts of flos carthami, 8 to 12 parts of American ginseng, 1 to 3 parts of dendrobium nobile and 10 to 15 parts of liquorice roots. The dendrobium candidum tender stem lozenge has the advantages that the dendrobium candidum tender stem is combined with the American ginseng, the safflower and other components, so that the anti-aging and immunity-enhancing effects of the dendrobium candidum tender stem lozenge are obviously improved, the good taste is kept, and the dendrobium candidum tender stem lozenge can be widely applied to the health-care product industry and meets the requirements of various people taking the dendrobium candidum tender stem lozenge.

Description

Technical Field

[0001] The invention relates to the technical field of health care product development, and in particular to a Dendrobium officinale young stem lozenge, and a preparation method and application thereof. Background Art

[0002] Dendrobium officinale, also known as Dendrobium officinale, earring dendrobium, iron orchid, black knot grass, etc., is listed as the first of the "Nine Immortal Herbs of China". It tastes sweet and is slightly cold in nature. As a traditional precious Chinese medicinal material, it is rich in polysaccharides, alkaloids, amino acids and trace elements. It has immune regulation, anti-oxidation, and blood sugar lowering effects. At present, the Dendrobium officinale products on the market mainly include Dendrobium officinale soft capsules, Dendrobium officinale lozenges, etc., which are health foods made with Dendrobium officinale and American ginseng as the main raw materials.

[0003] Lozenges refer to compressed tablets that dissolve slowly in the mouth and can produce lasting medicinal effects in the mouth and pharynx. Lozenges are popular among consumers because of their excellent taste and convenient method of taking.

[0004] Patent technical document CN105476004B discloses a Dendrobium officinale lozenge and a preparation method thereof. The invention prepares a Dendrobium officinale composition by designing a proportion of raw materials such as tea, Dendrobium officinale, hyaluronic acid, nano-carbon powder, vitamin C, propyl cellulose, micro-powder silica gel, magnesium stearate, etc., so that the obtained Dendrobium officinale lozenge has a better taste and can significantly enhance human immunity, but people inevitably take a certain dose of additives when taking it.

[0005] Patent technical document CN104605343B discloses a Dendrobium officinale tablet and a preparation method thereof. The tablet is a solid tablet with a water content of less than 5% and a solid component of 100% Dendrobium officinale. The tablet uses 100% Dendrobium officinale ingredients without any auxiliary materials. During preparation, the Dendrobium officinale itself is used as a slurry as a binder for the first time to achieve the purpose of adding no auxiliary materials, highlighting the natural and additive-free advantages of the product. However, from the perspective of traditional Chinese medicine theory, such a single-ingredient tablet may lack a comprehensive conditioning effect, because although Dendrobium officinale itself has a high medicinal value, simply using it as a raw material to prepare tablets may not be able to exert its comprehensive conditioning effect in traditional Chinese medicine theory, and may have a certain negative impact on its health care function, and cannot fully exert its value. Summary of the invention

[0006] In view of this, the purpose of the present invention is to provide a Dendrobium officinale young stem lozenge and a preparation method and application thereof, so as to provide a Dendrobium officinale young stem lozenge with good taste and certain health care functions.

[0007] Based on the above purpose, the present invention provides a Dendrobium officinale tender stem composition, comprising the following raw materials in parts by weight: 90-120 parts of Dendrobium officinale tender stems, 3-6 parts of safflower, 8-12 parts of American ginseng, 1-3 parts of Dendrobium nobile, and 10-15 parts of licorice.

[0008] Preferably, the Dendrobium officinale tender stem composition comprises the following raw materials in parts by weight: 95-110 parts of Dendrobium officinale tender stem, 4-5 parts of safflower, 9-11 parts of American ginseng, 2-3 parts of Dendrobium nobile, and 12-14 parts of licorice.

[0009] Preferably, the young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and liquorice are all dry products.

[0010] Preferably, the Dendrobium officinale tender stem dry product is obtained by a low-temperature freeze-drying method, wherein the temperature of the low-temperature freeze-drying is -40 to -30°C and the time is 1 to 3 hours.

[0011] Preferably, the young stems of Dendrobium officinale are obtained by aseptically cultivating for 43 to 48 days using plant cell suspension culture technology.

[0012] Preferably, the present invention also provides a method for preparing a Dendrobium officinale young stem lozenge, wherein the lozenge is prepared from a Dendrobium officinale young stem composition and pharmaceutically acceptable excipients.

[0013] Furthermore, the present invention also provides a method for preparing a Dendrobium officinale young stem lozenge, comprising the following steps:

[0014] (1) Material collection: The young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and dried liquorice were cleaned with distilled water to remove dust, dried to constant weight at room temperature, and the young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and liquorice were weighed according to the formula;

[0015] (2) Preparation of powder: Cut the young stems of Dendrobium officinale, American ginseng, Dendrobium nobile and dried liquorice into small pieces, grind them together with safflower, and pass through a 80-100 mesh sieve to obtain powder;

[0016] (3) Sterilization: sterilize the prepared powder by infrared radiation;

[0017] (4) Tabletting: Add a binder to the sterilized powder and press it into tablets using a tablet press.

[0018] Preferably, the pulverizing and grinding in step (2) is performed using an ultrafine pulverizer, and the pulverizing and grinding time is 30 to 45 minutes.

[0019] Preferably, the adhesive in step (4) is one of starch paste, mannose or gelatin paste.

[0020] Preferably, when the binder in step (4) is starch paste, its addition amount is 8-12% of the total weight of the powder, preferably 10%.

[0021] Preferably, the Dendrobium officinale young stem composition is used in the preparation of health products for increasing immunity and / or resisting aging.

[0022] Beneficial effects of the present invention: the Dendrobium officinale tender stem lozenge disclosed in the present application can more significantly enhance the body's immunity through the compatibility of various components, and can have a certain anti-aging effect;

[0023] The Dendrobium officinale tender stem lozenge disclosed in the present application uses the tender stem part of Dendrobium officinale as the main raw material, and the health care function of the Dendrobium officinale tender stem lozenge is further improved through low-temperature freeze-drying treatment technology;

[0024] The raw materials for preparing the Dendrobium officinale young stem lozenges disclosed in the present application are natural and easily available, contain no additives, and the product is greener and healthier. DETAILED DESCRIPTION

[0025] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments.

[0026] Embodiment 1: A method for preparing a Dendrobium officinale tender stem lozenge, the specific steps are as follows:

[0027] (1) Material collection: The young stems of Dendrobium officinale obtained by aseptic cultivation for 45 days using plant cell suspension culture technology were kept at -30°C for 2 hours to obtain the dry stems of Dendrobium officinale. Subsequently, the young stems of Dendrobium officinale and the dry products of safflower, American ginseng, Dendrobium nobile and licorice were cleaned with distilled water to remove dust, dried to constant weight at room temperature, and the young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and licorice were weighed according to the formula;

[0028] (2) Preparation of powder: 95 g of young stems of Dendrobium officinale, 9 g of American ginseng, 2 g of Dendrobium nobile and 12 g of dried liquorice were cut into small pieces, and ground together with 4 g of safflower for 30 min, and then passed through an 80-mesh sieve to obtain powder;

[0029] (3) Sterilization: sterilize the prepared powder by infrared radiation;

[0030] (4) Tablet pressing: Add 12.2 g of starch paste to the sterilized powder and mix well, then put the mixture into a tablet press and press into tablets.

[0031] Embodiment 2: A method for preparing a Dendrobium officinale tender stem lozenge, the specific steps are as follows:

[0032] (1) Material collection: The young stems of Dendrobium officinale obtained by aseptic cultivation for 45 days using plant cell suspension culture technology were kept at -35°C for 1.5 hours to obtain the dry stems of Dendrobium officinale, and then the stems and dry products of safflower, American ginseng, Dendrobium nobile and licorice were cleaned with distilled water to remove dust, dried to constant weight at room temperature, and the young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and licorice were weighed according to the formula;

[0033] (2) Preparation of powder: 105 g of young stems of Dendrobium officinale, 10 g of American ginseng, 2.5 g of Dendrobium nobile and 13 g of dried liquorice were cut into small pieces, and ground together with 4.5 g of safflower for 40 min, and then passed through a 90-mesh sieve to obtain powder;

[0034] (3) Sterilization: sterilize the prepared powder by infrared radiation;

[0035] (4) Tablet pressing: Add 13.5 g of starch paste to the sterilized powder and mix well, then put the mixture into a tablet press and press into tablets.

[0036] Embodiment 3: A method for preparing a Dendrobium officinale tender stem lozenge, the specific steps are as follows:

[0037] (1) Material collection: The young stems of Dendrobium officinale obtained by aseptic cultivation for 45 days using plant cell suspension culture technology were kept at -40°C for 1 hour to obtain the dry stems of Dendrobium officinale. Subsequently, the stems and the dried products of safflower, American ginseng, Dendrobium nobile and licorice were cleaned with distilled water to remove dust, dried to constant weight at room temperature, and the young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and licorice were weighed according to the formula;

[0038] (2) Preparation of powder: 110 g of young stems of Dendrobium officinale, 11 g of American ginseng, 3 g of Dendrobium nobile and 14 g of dried liquorice were cut into small pieces, and ground together with 5 g of safflower for 45 min, and then passed through a 100-mesh sieve to obtain powder;

[0039] (3) Sterilization: sterilize the prepared powder by infrared radiation;

[0040] (4) Tablet pressing: Add 14.3 g of starch paste to the sterilized powder and mix well, then put the mixture into a tablet press and press into tablets.

[0041] Comparative Example 1: A method for preparing a Dendrobium officinale stem lozenge, which is different from Example 2 in that the drying method of the Dendrobium officinale stem is replaced by conventional heating and drying instead of low-temperature freeze drying, and the specific steps are as follows:

[0042] (1) Material collection: The young stems of Dendrobium officinale obtained by aseptic cultivation for 45 days using plant cell suspension culture technology were kept at 60° C. for 2 h to obtain the dry stems of Dendrobium officinale. The stems and the dried products of safflower, American ginseng, Dendrobium nobile and licorice were then cleaned with distilled water to remove dust and dried at room temperature to constant weight. The young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and licorice were weighed according to the formula;

[0043] (2) Preparation of powder: 105 g of young stems of Dendrobium officinale, 10 g of American ginseng, 2.5 g of Dendrobium nobile and 13 g of dried liquorice were cut into small pieces, and ground together with 4.5 g of safflower for 40 min, and then passed through a 90-mesh sieve to obtain powder;

[0044] (3) Sterilization: sterilize the prepared powder by infrared radiation;

[0045] (4) Tablet pressing: Add 13.5 g of starch paste to the sterilized powder and mix well, then put the mixture into a tablet press and press into tablets.

[0046] Performance Testing

[0047] Sensory test: Take samples for testing on the day of production and 30 days after production (stored according to market conditions). The testing method is as follows: Take 5 pieces at random. Observe their shape, smell and taste, and make evaluations. The test results are shown in Table 1:

[0048] Table 1 Sensory test results

[0049]

[0050] Anti-aging function test: Kunming mice, male, 4 weeks old; Kunming mice were adaptively fed in a laboratory at 22-25°C, relative humidity 55%-70%, 12h light on and off cycle for one week; 60 rats were randomly divided into a blank group, a model group, Example 1-3 groups, and a comparative example group 1, a total of 6 groups, 10 rats in each group. The blank group was gavaged with distilled water every day, and the same volume of normal saline was intraperitoneally injected; the model group was gavaged with distilled water every day, and D-galactose (300 mg / kg) was intraperitoneally injected; the example group was gavaged with a distilled water suspension of the Dendrobium officinale stem lozenge obtained in the corresponding example every day (2g lozenge was crushed and suspended with 10mL distilled water, the same below), and an equal amount of D-galactose was intraperitoneally injected; the comparative example group was gavaged with a distilled water suspension of the Dendrobium officinale stem lozenge obtained in the comparative example every day, and an equal amount of D-galactose was intraperitoneally injected; all mice were fed with standard animal feed, free access to food and water, and the body weight of the mice was weighed during the experiment. At the end of the experiment on the 43rd day, all animals were anesthetized and killed, and the mouse liver tissues were collected for the determination of total superoxide dismutase (T-SOD) content. SPSS20.0 was used to analyze the data, and the significant difference level was set at 0.05 (P < 0.05, significant difference). The test results are shown in Tables 2 and 3.

[0051] Table 2 Changes in mouse body weight

[0052]

[0053]

[0054] The results of the weight change test are shown in Table 2: During the experiment, the body weight growth of the model mice slowed down compared with the blank group mice, and the vitality of the mice in the Example 1-3 group and the comparative example 1 group gradually recovered, and the body weight growth gradually returned to normal; but the recovery degree of the comparative example 1 group was less than that of the Example 2. The above results show that the Dendrobium officinale tender stem lozenges disclosed in the present application have certain anti-aging functions.

[0055] Table 3 T-SOD levels in mouse liver tissue (mean ± SD, n = 10)

[0056] Group U / mg ptot Example 1 46.65±3.97* Example 2 45.22±3.04* Example 3 46.17±3.78* Comparative Example 1 41.33±3.16* Blank Group 41.08±2.75* Model Group 28.76±3.34

[0057] Note: * indicates significant difference compared with the model group, P < 0.05

[0058] The test results of T-SOD levels in mouse liver tissue are shown in Table 3: It can be seen from the results that D-galactose intervention leads to a decrease in the T-SOD content in mouse liver tissue, indicating that the modeling is successful. The lozenges prepared in Examples 1-3 and Comparative Example 1 can restore the T-SOD level in the liver tissue of the model mice; but the degree of recovery in Comparative Example 1 is less than that in Example 2. The above results show that the Dendrobium officinale stem lozenges disclosed in the present application have a certain protective effect on the aging process of mice induced by D-galactose, and also reflect that the Dendrobium officinale stem lozenges have a certain anti-aging function, and compared with the dry product of Dendrobium officinale stem obtained by heating and drying, the low-temperature freeze-drying treatment of Dendrobium officinale stem can further enhance the anti-aging function of the final lozenge product.

[0059] Immune function test: Kunming mice, male, 4 weeks old; Kunming mice were adaptively fed in a laboratory at 22-25°C, relative humidity 55%-70%, 12h light on and light off cycle for one week; 60 rats were randomly divided into 6 groups, 10 rats in each group: blank group, model group, Example 1-3 group, and Comparative Example 1 group. After grouping, Example 1-3 group and Comparative Example 1 group were intragastrically administered with a distilled water suspension of Dendrobium officinale tender stem lozenges prepared in the corresponding examples and comparative examples (2g lozenges were crushed and suspended with 10mL distilled water, the same below), and the blank group and the model group were given an equal amount of water, once a day, for 30 consecutive days. Except for the blank group, mice in other groups were modeled on the 19th, 21st, 23rd, 25th and 27th days of administration, and 2.5mg / mL cyclophosphamide solution was injected intraperitoneally at a dose of 0.2ml / 10g·bw, and the blank group was injected intraperitoneally with normal saline at a dose of 0.2mL / 10g·bw, and the injection was 5 times in total to establish an immunosuppressive mouse model. 24h after the last administration, the mice were killed by cervical dislocation, and the spleen and thymus were dissected and placed in normal saline to wash the blood. The surface moisture of the organs was dried with filter paper, and the organ weight was weighed after the connective tissue was removed. The immune organ index was calculated according to the formula: immune organ index (mg / g) = immune organ weight (mg) / body weight of mice before death (g). SPSS20.0 was used to analyze the data, and the significant difference level was set to 0.05 (P < 0.05, significant difference). The test results are shown in Table 4.

[0060] Table 4 Mouse immune organ index (mean ± SD, n = 10)

[0061] Group Spleen index Thymus index Example 1 3.21±0.10* 1.60±0.07* Example 2 3.15±0.08* 1.48±0.06* Example 3 3.20±0.09* 1.55±0.05* Comparative Example 1 3.09±0.08* 1.45±0.05* Blank Group 3.18±0.10* 1.52±0.09* Model Group 2.43±0.07 0.82±0.05

[0062] Note: * indicates significant difference compared with the model group, P < 0.05

[0063] The test results of mouse immune organ index are shown in Table 4: It can be seen from the results that compared with the blank group, the spleen and thymus indexes of the model group were significantly reduced, indicating that the modeling was successful. The lozenges prepared in Examples 1-3 and Comparative Example 1 can restore the immune organ indexes of the model mice; but the degree of recovery in Comparative Example 1 is less than that in Example 2. The above results show that the Dendrobium officinale tender stem lozenges disclosed in the present application can improve the damage of the immune organs of mice induced by immunosuppressants and enhance the immune function of mice, and low-temperature freeze-drying of the Dendrobium officinale tender stems can better maintain the immune-enhancing effect of the Dendrobium officinale tender stem lozenges.

[0064] Those skilled in the art should understand that the discussion of any of the above embodiments is merely illustrative and is not intended to imply that the scope of the present invention is limited to these examples. Under the concept of the present invention, the technical features in the above embodiments or different embodiments may be combined, the steps may be implemented in any order, and there are many other variations of the different aspects of the present invention as described above, which are not provided in detail for the sake of simplicity.

Claims

1. A Dendrobium officinale tender stem composition, characterized in that: The invention comprises the following raw materials in parts by weight: 90-120 parts of young stems of dendrobium candidum, 3-6 parts of safflower, 8-12 parts of American ginseng, 1-3 parts of dendrobium nobile and 10-15 parts of liquorice.

2. The Dendrobium officinale tender stem composition according to claim 1, characterized in that: The Dendrobium officinale tender stem composition comprises the following raw materials in parts by weight: 95-110 parts of Dendrobium officinale tender stems, 4-5 parts of safflower, 9-11 parts of American ginseng, 2-3 parts of Dendrobium nobile, and 12-14 parts of liquorice.

3. The Dendrobium officinale tender stem composition according to claim 1, characterized in that: The young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and liquorice are all dry products.

4. The Dendrobium officinale tender stem composition according to claim 3, characterized in that: The Dendrobium officinale tender stem dry product is obtained by a low-temperature freeze-drying method, wherein the temperature of the low-temperature freeze-drying is -40 to -30°C and the time is 1 to 3 hours.

5. The Dendrobium officinale tender stem composition according to claim 1, characterized in that: The young stems of Dendrobium officinale are obtained by aseptically cultivating for 43 to 48 days using plant cell suspension culture technology.

6. A Dendrobium officinale young stem lozenge, characterized in that: The lozenge is prepared from the Dendrobium officinale tender stem composition according to any one of claims 1 to 5 and pharmaceutically acceptable excipients.

7. A method for preparing the Dendrobium officinale young stem lozenge according to claim 6, characterized in that: The following steps are involved: (1) Material collection: The young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and dried liquorice were cleaned with distilled water to remove dust, dried to constant weight at room temperature, and the young stems of Dendrobium officinale, safflower, American ginseng, Dendrobium nobile and liquorice were weighed according to the formula; (2) Preparation of powder: Cut the young stems of Dendrobium officinale, American ginseng, Dendrobium nobile and dried liquorice into small pieces, grind them together with safflower, and pass through a 80-100 mesh sieve to obtain powder; (3) Sterilization: sterilize the prepared powder by infrared radiation; (4) Tablet pressing: Add a binder to the sterilized powder and press it into tablets in a tablet press.

8. The method for preparing the Dendrobium officinale young stem lozenge according to claim 7, characterized in that: The pulverizing and grinding in step (2) is performed using an ultrafine pulverizer, and the pulverizing and grinding time is 30 to 45 minutes.

9. The method for preparing the Dendrobium officinale young stem lozenge according to claim 7, characterized in that: The adhesive in step (4) is one of starch paste, mannose or gelatin paste.

10. An application of the Dendrobium officinale tender stem composition according to any one of claims 1 to 5, characterized in that: Application in the preparation of health products for enhancing immunity and / or resisting aging.

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