Traditional Chinese medicine composition and application thereof in preparation of medicine with anxiety relieving effect

Abstract

The invention relates to the technical field of biological medicines, and particularly discloses a composition which comprises the following components in parts by weight: 1-10 parts of tea tree essential oil; 1-10 parts of Chinese mugwort essential oil; and 1-10 parts of lemon essential oil. Researches show that the composition containing the tea tree essential oil, the Chinese mugwort essential oil and the lemon essential oil has the effect of relieving anxiety; therefore, the compound can be further used as an effective component for preparing a medicine with an anxiety relieving effect. In addition, the composition disclosed by the invention contains essential oil with a volatile effect; therefore, the effect of relieving anxiety can be achieved through aromatherapy in the application process.

Description

Technical Field

[0001] The present invention relates to the technical field of biomedicine, and in particular to a composition and a preparation method thereof, and application of the composition in preparing a drug having an anxiety-relieving effect. Background Art

[0002] Anxiety disorder is a common psychological disorder, with the main symptoms of intense, excessive and persistent worry and fear, nervousness accompanied by increased heart rate, difficulty sleeping, intestinal problems, etc. It is caused by the combined effects of biological, psychological and social factors. The treatment methods for anxiety disorder mainly include psychotherapy and drug therapy.

[0003] Chinese invention patent CN202410154234.5 discloses a stomach-warming and analgesic external application composition and a stomach-warming and analgesic plaster and a preparation method; the stomach-warming and analgesic external application composition includes the following components in parts by mass: 10-40 parts of mugwort, 10-40 parts of raw Chuanwu, 10-40 parts of raw Caowu, 10-40 parts of cinnamon twigs, 10-40 parts of mulberry branches, 10-40 parts of Liujinu, 10-40 parts of Chuanxiong, 10-40 parts of papaya, 10-40 parts of roasted frankincense, 10-40 parts of roasted myrrh, 10-40 parts of Clematis, 10-40 parts of Qiannianjian, 10-40 parts of Zanthoxylum bungeanum, and 10-40 parts of Chuanxiong. The stomach-warming and analgesic plaster of the present invention has the effects of strengthening the spleen and warming the middle, removing dampness and dispersing cold, nourishing the heart and calming the mind through the synergistic effect of various raw materials, and treats epigastric pain, epigastric fullness, insomnia and the accompanying anxiety, depression, fatigue, neurasthenia and the like caused by chronic gastritis from multiple aspects.

[0004] Although the plaster made from the above-mentioned Chinese medicine composition has a certain effect in treating anxiety, its effect in treating anxiety is not its main efficacy and needs further improvement. Summary of the invention

[0005] In order to overcome the technical problems existing in the prior art, the present invention first provides a composition; The above technical problem to be solved by the present invention is achieved by adopting the following technical solution: The present invention first provides a composition comprising the following components in parts by weight: 1-10 parts of tea tree essential oil; 1-10 parts of Artemisia selengensis essential oil; 1-10 parts of lemon essential oil.

[0006] Preferably, the composition comprises the following components in parts by weight: 8 parts of tea tree essential oil; 5 parts of Artemisia selengensis essential oil; 5 parts of lemon essential oil.

[0007] The invention provides a new composition. Studies have shown that the composition comprising tea tree essential oil, Artemisia selengensis essential oil and lemon essential oil has the effect of alleviating anxiety.

[0008] Preferably, the composition further comprises an extract; The extract is prepared by taking cinnamon bark and eucommia bark as raw materials.

[0009] The inventors found in further research that further adding extracts prepared with cinnamon and eucommia as raw materials to a composition comprising tea tree essential oil, Artemisia selengensis essential oil and lemon essential oil can further significantly enhance the anxiety-relieving effect of the composition.

[0010] Preferably, the following components are included in parts by weight: 1-10 parts of tea tree essential oil; 1-10 parts of Artemisia selengensis essential oil; 1-10 parts of lemon essential oil; 1-5 parts of extract.

[0011] Most preferably, the following components are included in weight: 8 parts of tea tree essential oil; 5 parts of Artemisia selengensis essential oil; 5 parts of lemon essential oil; 3 parts of extract.

[0012] Preferably, the extract is prepared by the following method: The Chinese medicinal materials cinnamon bark and eucommia bark are extracted with a solvent, and after the extraction is completed, the extract is filtered and separated, and the extract is concentrated and dried to obtain a solvent extract, and the solvent extract is taken to obtain the extract.

[0013] Preferably, the solvent is acetone.

[0014] Preferably, the weight ratio of the Chinese medicine cinnamon bark and eucommia bark is 1-3:1-3.

[0015] Most preferably, the weight ratio of the Chinese medicine cinnamon bark and eucommia bark is 1:1.

[0016] Preferably, the total weight ratio of the Chinese medicine cinnamon bark and eucommia bark to the solvent is 1kg:5-10L.

[0017] The usage ratio of the total weight of the Chinese medicinal herbs cinnamon bark and eucommia bark to the solvent is 1kg:8L.

[0018] Preferably, the extraction is heating reflux extraction; the extraction time is 1 to 3 hours.

[0019] Further preferably, the preparation method of the extract further comprises the following steps: applying the solvent extract to a silica gel column, eluting with a mixed solvent consisting of petroleum ether and acetone in a volume ratio of 100:5-10, and collecting the eluate after the elution is completed, concentrating and drying to obtain the extract.

[0020] Most preferably, the method for preparing the extract further comprises the following steps: applying the solvent extract to a silica gel column, eluting with a mixed solvent consisting of petroleum ether and acetone in a volume ratio of 100:7, and collecting the eluate after the elution is completed, concentrating and drying to obtain the extract.

[0021] The inventors found in further research that further adding an extract obtained after elution with the above-mentioned silica gel column to a composition comprising tea tree essential oil, Artemisia argyi essential oil and lemon essential oil can further significantly enhance the anxiety-relieving effect of the composition compared to adding a solvent extract obtained without elution with the above-mentioned silica gel column.

[0022] The inventors also found in the study that the composition of the elution solvent is very critical in the elution process of the above-mentioned silica gel column; the study shows that in the composition comprising tea tree essential oil, Artemisia argyi essential oil and lemon essential oil, only by further adding the extract obtained by elution with the elution solvent of the above composition, can the anxiety-relieving effect of the composition be further significantly improved compared with the addition of the solvent extract obtained without elution with the above-mentioned silica gel column; however, adding the extract obtained by elution with elution solvents of other compositions cannot further significantly improve the anxiety-relieving effect of the composition.

[0023] The present invention also provides an application of the Chinese medicine composition in preparing a medicine having an anxiety-relieving effect.

[0024] Beneficial effects: The present invention provides a new composition. Studies have shown that the composition comprising tea tree essential oil, Artemisia selengensis essential oil and lemon essential oil has an anxiety-relieving effect; therefore, it can be further used as an effective ingredient to prepare a drug with an anxiety-relieving effect. In addition, since the composition of the present invention comprises an essential oil with a volatile effect, aromatherapy can be used to achieve an anxiety-relieving effect during its application. DETAILED DESCRIPTION

[0025] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with specific embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] The tea tree essential oil, Artemisia argyi essential oil and lemon essential oil described in the following examples are all prepared by conventional steam distillation.

[0027] Example 1 Preparation of composition 8 parts of tea tree essential oil, 5 parts of Artemisia selengensis essential oil and 5 parts of lemon essential oil are mixed evenly to obtain the composition.

[0028] Example 2 Preparation of composition 8 parts of tea tree essential oil, 5 parts of Artemisia selengensis essential oil, 5 parts of lemon essential oil and 3 parts of extract are mixed evenly to obtain the composition; The extract is prepared by the following method: Take Chinese medicinal herbs cinnamon and eucommia bark and heat them to boiling with a solvent under normal pressure and then extract them for 1.5 hours. After the extraction is completed, filter and separate the extract, concentrate and dry the extract to obtain a solvent extract, and take the solvent extract to obtain the extract; The solvent is acetone; the weight ratio of the Chinese medicinal herbs cinnamon and eucommia bark is 1:1; and the dosage ratio of the total weight of the Chinese medicinal herbs cinnamon and eucommia bark to the solvent is 1kg:8L.

[0029] Example 3 Preparation of composition 8 parts of tea tree essential oil, 5 parts of Artemisia selengensis essential oil, 5 parts of lemon essential oil and 3 parts of extract are mixed evenly to obtain the composition; The extract is prepared by the following method: (1) taking Chinese medicinal herbs cinnamon and eucommia bark and heating them to boiling with a solvent under normal pressure and then extracting them for 1.5 hours, filtering and separating the extract after the extraction, concentrating and drying the extract to obtain a solvent extract; wherein the solvent is acetone; the weight ratio of the Chinese medicinal herbs cinnamon and eucommia bark is 1:1; and the total weight ratio of the Chinese medicinal herbs cinnamon and eucommia bark to the solvent is 1kg:8L; (2) 30 times the weight of the solvent extract and 200-300 mesh silica gel are loaded into a glass column to obtain a silica gel column; then the solvent extract is loaded onto the silica gel column, and then eluted with a mixed solvent consisting of petroleum ether and acetone in a volume ratio of 100:7 with a volume ratio of 3 times the column volume. After the elution is completed, the eluate is collected, concentrated and dried to obtain the extract.

[0030] Comparative Example 1 Preparation of the composition 8 parts of tea tree essential oil, 5 parts of Artemisia selengensis essential oil, 5 parts of lemon essential oil and 3 parts of extract are mixed evenly to obtain the composition; The extract is prepared by the following method: Take the Chinese medicinal material cinnamon and heat it to boiling with a solvent under normal pressure, then extract it for 1.5 hours. After the extraction is completed, filter and separate the extract, concentrate and dry the extract to obtain a solvent extract, and take the solvent extract to obtain the extract; Wherein, the solvent is acetone; the dosage ratio of the Chinese medicine cinnamon to the solvent is 1kg:8L.

[0031] Comparative Example 2 Preparation of the composition 8 parts of tea tree essential oil, 5 parts of Artemisia selengensis essential oil, 5 parts of lemon essential oil and 3 parts of extract are mixed evenly to obtain the composition; The extract is prepared by the following method: Take the Chinese medicinal material Eucommia ulmoides and heat it to boiling with a solvent under normal pressure and then extract it for 1.5 hours. After the extraction is completed, filter and separate the extract, concentrate and dry the extract to obtain a solvent extract, and take the solvent extract to obtain the extract; The solvent is acetone; the dosage ratio of the Chinese medicine Eucommia ulmoides to the solvent is 1kg:8L.

[0032] Comparative Example 3 Preparation of Composition 8 parts of tea tree essential oil, 5 parts of Artemisia selengensis essential oil, 5 parts of lemon essential oil and 3 parts of extract are mixed evenly to obtain the composition; The extract is prepared by the following method: (1) taking Chinese medicinal herbs cinnamon and eucommia bark and heating them to boiling with a solvent under normal pressure and then extracting them for 1.5 hours, filtering and separating the extract after the extraction, concentrating and drying the extract to obtain a solvent extract; wherein the solvent is acetone; the weight ratio of the Chinese medicinal herbs cinnamon and eucommia bark is 1:1; and the total weight ratio of the Chinese medicinal herbs cinnamon and eucommia bark to the solvent is 1kg:8L; (2) 30 times the weight of the solvent extract and 200-300 mesh silica gel are loaded into a glass column to obtain a silica gel column; then the solvent extract is loaded onto the silica gel column, and then eluted with a mixed solvent consisting of petroleum ether and acetone in a volume ratio of 100:20 with a volume ratio of 3 times the column volume. After the elution is completed, the eluate is collected, concentrated and dried to obtain the extract.

[0033] Comparative Example 4 Preparation of Composition 8 parts of tea tree essential oil, 5 parts of Artemisia selengensis essential oil, 5 parts of lemon essential oil and 3 parts of extract are mixed evenly to obtain the composition; The extract is prepared by the following method: (1) taking Chinese medicinal herbs cinnamon and eucommia bark and heating them to boiling with a solvent under normal pressure and then extracting them for 1.5 hours, filtering and separating the extract after the extraction, concentrating and drying the extract to obtain a solvent extract; wherein the solvent is acetone; the weight ratio of the Chinese medicinal herbs cinnamon and eucommia bark is 1:1; and the total weight ratio of the Chinese medicinal herbs cinnamon and eucommia bark to the solvent is 1kg:8L; (2) 30 times the weight of the solvent extract and 200-300 mesh silica gel are loaded into a glass column to obtain a silica gel column; then the solvent extract is loaded onto the silica gel column and then eluted with 3 times the column volume of petroleum ether. After the elution is completed, the eluate is collected, concentrated and dried to obtain the extract.

[0034] Experimental Example 1: Mouse elevated plus maze test The elevated plus maze used in this experiment includes a central platform, two open arms, and two closed arms. Mice tend to move in the closed arms of the elevated plus maze due to their scotopic nature, but they also move in the open arms out of curiosity and exploratory nature. When faced with novel stimuli, animals will have the urge and fear to explore, which creates a conflict between exploration and avoidance, resulting in anxiety, which in turn prompts mice to be more inclined to move in the closed arms. Mice that overcome anxiety will move more frequently in the open arms. Based on this principle, we use the percentage of times entering the open arms as an evaluation of the anxiety-relieving effect. The higher the percentage of times mice enter the open arms, the better the anxiety-relieving effect.

[0035] 80 male Kunming mice weighing 25±2g were selected and randomly divided into 8 groups, each with 10 mice; including a blank control group and experimental groups 1-7; wherein the experimental groups 1-7 were smeared with the Chinese medicine composition prepared in Examples 1-3 and Comparative Examples 1-4 (at a dose of 15 mg / kg) on ​​the abdomen of the mice once a day; after continuous smearing for 30 days; the mice were placed on the central platform of an elevated plus maze, with their heads facing the open arms, illuminated by work lights, and allowed to explore freely, and the number of times each group of mice entered the open arms and the closed arms within 5 minutes was recorded, and the percentage of the number of times each group of mice entered the open arms was calculated; the experimental results are shown in Table 1.

[0036] Percentage of times entering the open arm = [times entering the open arm / (times entering the open arm + times entering the closed arm)] 100%.

[0037] Table 1.

[0038] The experimental results in Table 1 show that the percentage of mice in the experimental group 1 entering the open arms is significantly higher than that in the blank control group; this indicates that the composition comprising tea tree essential oil, Artemisia selengensis essential oil and lemon essential oil of the present invention has an anxiety-relieving effect.

[0039] The experimental results in Table 1 show that the percentage of mice entering the open arms in the experimental group 2 was significantly higher than that in the Experimental Group 1: This indicates that: in the composition comprising tea tree essential oil, Artemisia selengensis essential oil and lemon essential oil, further adding an extract prepared with cinnamon bark and Eucommia ulmoides as raw materials can further significantly enhance the anxiety-relieving effect of the composition.

[0040] The experimental results in Table 1 show that the percentage of the number of times the mice in experimental groups 4 to 6 entered the open arms was not much improved compared with that in experimental group 1, and the improvement was much smaller than that in experimental group 2; this indicates that in the composition comprising tea tree essential oil, Artemisia argyi essential oil and lemon essential oil, extracts prepared with cinnamon and Eucommia ulmoides as raw materials must be further added simultaneously to further significantly improve the anxiety-relieving effect of the composition; however, only adding extracts prepared with cinnamon as raw material, or only adding extracts prepared with Eucommia ulmoides as raw material, cannot further significantly improve the anxiety-relieving effect of the composition.

[0041] The experimental results in Table 1 show that the percentage of mice entering the open arms in the experimental group 3 is significantly higher than that in the Experimental Group 2: This shows that: in the composition comprising tea tree essential oil, Artemisia argyi essential oil and lemon essential oil, further adding the extract obtained after elution by the silica gel column of the present invention can further significantly enhance the anxiety-relieving effect of the composition compared with adding the solvent extract obtained without elution by the above-mentioned silica gel column.

[0042] The experimental results in Table 1 show that the percentage of mice entering the open arms in experimental groups 7 and 8 did not increase much compared with experimental group 2, and the increase was much smaller than that in experimental group 3; this indicates that: In the elution process of the silica gel column of the present invention, the composition of the elution solvent is very critical; the above experimental results show that in the composition comprising tea tree essential oil, Artemisia argyi essential oil and lemon essential oil, only by further adding the extract obtained by elution with the elution solvent of the above composition of the present invention, compared with adding the solvent extract obtained without elution with the above silica gel column, can the anxiety-relieving effect of the composition be further significantly improved; however, adding the extract obtained by elution with the elution solvent of other compositions cannot further significantly improve the anxiety-relieving effect of the composition.

[0043] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0044] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A composition, characterized in that Contains the following components in parts by weight: 1-10 parts of tea tree essential oil; 1-10 parts of Artemisia selengensis essential oil; 1-10 parts of lemon essential oil.

2. The composition according to claim 1, characterized in that Contains the following components in parts by weight: 8 parts of tea tree essential oil; 5 parts of Artemisia selengensis essential oil; 5 parts of lemon essential oil.

3. The composition according to claim 1, characterized in that The composition further comprises an extract; The extract is prepared by taking cinnamon bark and eucommia bark as raw materials.

4. The composition according to claim 4, characterized in that Contains the following components in parts by weight: 1-10 parts of tea tree essential oil; 1-10 parts of Artemisia selengensis essential oil; 1-10 parts of lemon essential oil; 1-5 parts of extract.

5. The composition according to claim 4, characterized in that Contains the following components in parts by weight: 8 parts of tea tree essential oil; 5 parts of Artemisia selengensis essential oil; 5 parts of lemon essential oil; 3 parts of extract.

6. The composition according to claim 5, characterized in that The extract is prepared by the following method: The Chinese medicinal materials cinnamon bark and eucommia bark are extracted with a solvent, and after the extraction is completed, the extract is filtered and separated, and the extract is concentrated and dried to obtain a solvent extract, and the solvent extract is taken to obtain the extract.

7. The composition according to claim 6, characterized in that The solvent is acetone.

8. The composition according to claim 6, characterized in that The weight ratio of Chinese medicine cinnamon bark and eucommia bark is 1~3:1~3; Most preferably, the weight ratio of the Chinese medicine cinnamon bark and eucommia bark is 1:

1.

9. The composition according to claim 6, characterized in that The total weight of the Chinese medicine cinnamon and eucommia bark to the solvent is 1kg:5-10L; The usage ratio of the total weight of the Chinese medicinal herbs cinnamon bark and eucommia bark to the solvent is 1kg:8L.

10. Use of the Chinese medicine composition according to any one of claims 1 to 9 in the preparation of a drug having an anxiety-relieving effect.

Images