Insecticide composition for preventing and treating root-knot nematode and application thereof

Abstract

The invention relates to an insecticide composition for preventing and treating root-knot nematodes and application thereof, and belongs to the field of agricultural nematode killing compositions. The effective components of the nematicidal composition provided by the invention are compounded by two spirotetramat and hymexazol, and the weight ratio of two spirotetramat to hymexazol is (1: 80)-(80: 1), preferably (1: 20)-(20: 1). A preparation prepared from the compound has very high activity on root-knot nematodes; the composition is not simple superposition of the activities of the two components, but has a remarkable synergistic effect; the field dosage of the effective components is reduced, the production and use cost is reduced, and pesticide residues and environmental pollution are reduced; the composition is composed of effective components with different action mechanisms, and the action mechanisms complement each other, so that the composition is beneficial to overcoming and delaying the generation of pest resistance to drugs, and is safe to crops.

Description

Technical Field

[0001] The invention belongs to the field of agricultural pesticides, and particularly relates to a pesticide composition for preventing and controlling root-knot nematodes and an application thereof. Background Art

[0002] With the promotion and extensive use of pesticides, the disadvantages of single active ingredient pesticides in agricultural pesticide control, such as narrow insecticide range, poor control effect, high dosage, easy to produce resistance and pesticide damage, frequent application, aggravated environmental pollution, etc., have obviously led to a large reduction in crop yields and a substantial increase in production costs. One way to prevent and control resistant pests in agriculture is to introduce new ingredients that have no cross-resistance with existing varieties. However, the development cost of new active ingredients is high, the development cycle is long, and they can never keep up with the speed at which pests develop resistance. Other methods, such as crop layout adjustment and rotation of different pesticides, are difficult to really achieve significant results in actual operations.

[0003] Root-knot nematodes are a common soil-borne pathogen that parasitizes in the root system of plants and directly harms the roots of crops. The wounds caused by them can cause secondary infections of other fungi, bacteria and other microorganisms, aggravating the occurrence of soil-borne diseases, resulting in tumor-like protrusions on the roots of the affected crops, fewer fibrous roots, and slow growth of the aboveground parts, dwarfing and yellowing of the plants, and in severe cases, growth stagnation or even death. However, due to its small size, fast breeding speed, difficult diagnosis, few control agents, and strong drug resistance of old products, root-knot nematodes have become one of the most difficult pests to control in current crop production. In order to control root-knot nematodes, there are currently a variety of pesticides available on the market. However, conventional pesticides have problems such as low safety, large dosage, environmental pollution, severe resistance, and poor effect, which have always plagued pesticide companies and farmers. Therefore, how to effectively control root-knot nematodes while ensuring the quality and safety of agricultural products is an urgent problem to be solved.

[0004] At present, agricultural pest control requires the combination of pesticides, which often have improved characteristics such as insecticide synergistic effect. Compared with the long development cycle of new pesticides, the research of high-efficiency and low-toxic compound compositions has the advantages of small investment and short cycle, and therefore continues to attract the attention of pesticide researchers at home and abroad.

[0005] Dispiracil is a new type of insecticide with spirocyclic tetrakis acid and tetramic acid structure developed by Bayer CropScience, with CAS registration number 907187-07-9. It is speculated that the mechanism of action of Dispiracil should be similar to that of spirotetramat and methoxyfentamethylenetetrakis ethyl, both of which belong to the class of acetyl-CoA carboxylase (ACCase) inhibitors; the mechanism of action is a lipid biosynthesis inhibitor, which inhibits the activity of ACCase in the process of fat synthesis in the pest body, destroys lipid synthesis, thereby blocking the normal energy metabolism of the pest, and ultimately leading to the death of the pest. Unlike other tetramic acid insecticides, one of the carbonyl groups in Dispiracil exists as a free hydroxyl group rather than a protected precursor. It has good permeability when applied to crops and is effective against target pests at low doses.

[0006] Oxymexazol belongs to a new generation of new pesticide fungicide, which is systemic and can be used as a soil disinfectant. It is green, environmentally friendly, low-toxic and pollution-free. After entering the soil, oxymexazol is absorbed by the soil and combines with inorganic metal salt ions such as iron and aluminum in the soil, effectively inhibiting the germination of spores and the normal growth of pathogenic fungal mycelium or directly killing pathogens, and the efficacy can reach two weeks. It can effectively inhibit the normal growth of pathogenic fungal mycelium or directly kill pathogens, and has special effects on wilt, verticillium wilt, damping-off disease, sheath blight, leaf blight, and continuous cropping obstacles. At the same time, oxymexazol is also a plant growth regulator, which can be absorbed by the roots of plants and move in the root system, and metabolize to produce two glycosides, which have the effect of improving the physiological activity of crops, thereby promoting plant growth, root tillering, increase of root hairs and improvement of root activity. Because it has little effect on bacteria and actinomycetes other than pathogens in the soil, it has no effect on the ecology of microorganisms in the soil, and can be decomposed into compounds with very low toxicity in the soil, which is safe for the environment.

[0007] However, there are no reports on the combination of the two for the control of root-knot nematodes. In addition, the varieties of nematicidal compositions containing a single active ingredient often have defects to varying degrees in the control of agricultural nematicidal compositions. If different components with synergistic effects are rationally compounded, the purpose of effectively controlling crop pests can be achieved. Compounding of pesticides can expand the insecticide spectrum, improve the control effect, expand the control range, reduce the amount of active ingredients, save the cost of medication, and reduce environmental pollution. Therefore, the research and development of efficient, low-toxic, and environmentally friendly nematicidal compositions has a positive effect on the sustainable development of agriculture. Summary of the invention

[0008] The invention provides an insecticide composition for controlling root-knot nematodes and application thereof in controlling nematode infection and development on crops. The nematicidal composition has obvious synergistic effect, low cost, good efficacy, long lasting effect and little environmental pollution.

[0009] In order to achieve the above object, an insecticide composition for controlling root-knot nematodes is provided, wherein the active ingredients are composed of a compound of dimethoate and oxazolidinone, and the weight ratio of the two is 1:80 to 80:1, preferably 1:20 to 20:1.

[0010] Preferably, in the above nematicidal composition, the weight percentage of the effective ingredients dispirilla and oxazolidinone in the nematicidal composition is 1% to 90%, preferably 10% to 60%.

[0011] The content of the active ingredient in the composition of the present invention depends on the application amount when used alone, the mixing ratio of one compound to another compound, the degree of synergistic effect, and is also related to the target disease. Usually, the weight percentage of the active ingredient in the composition is 1% to 90% of the total weight, preferably 5% to 80%. Depending on the type of preparation, the range of active ingredient content varies. Usually, liquid preparations contain 1% to 60% of active substances by weight, preferably 5% to 50%; solid preparations contain 5% to 80% of active substances by weight, preferably 10% to 80%.

[0012] Preferably, in addition to dispiracil and oxazolidinone, the nematicidal composition also includes auxiliary ingredients for agricultural preparations, which include carriers, solvents, fillers and adjuvants.

[0013] Preferably, the carrier is selected from at least one of clay particles, broken brick particles, sand, plant-derived carriers, and fertilizers;

[0014] The solvent is selected from at least one of organic solvents such as solvent oil, nitrogen methyl pyrrolidone, acetophenone, cyclohexanone, dimethyl sulfoxide, and plant-derived environmentally friendly solvents such as turpentine, methyl oleate, soybean oil, corn oil, and rapeseed oil;

[0015] The filler is selected from at least one of kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch, and light calcium carbonate;

[0016] The adjuvant includes at least one surfactant to facilitate the dispersion of the active ingredient in water during application. The surfactant is selected from at least one of an emulsifier, a dispersant, a wetting agent, and a penetrant, and its content is 2% to 30% of the total weight of the preparation, and the balance is a solid or liquid diluent. It is understood that the above-mentioned surfactant is a single agent or a compound preparation of a common nonionic surfactant or anionic surfactant.

[0017] Preferably, according to different usage occasions and requirements, the auxiliary agent may also include other functional auxiliary agents, which are selected from one or more of antifreeze agents, thickeners, stabilizers, disintegrants, defoamers, warning colors, and film-forming agents.

[0018] The emulsifier is selected from Nongru 500# (calcium dodecylbenzene sulfonate), tristyrylphenol polyoxyethylene ether, OP series phosphate (nonylphenol polyoxyethylene ether phosphate), 600# phosphate (phenylphenol polyoxyethylene ether phosphate), styrene polyoxyethylene ether ammonium sulfate, alkyl biphenyl ether disulfonic acid magnesium salt, triethanolamine salt, Nongru 400# (benzyl dimethylphenol polyoxyethyl ether), Nongru 700# (alkylphenol formaldehyde resin polyoxyethyl ether), Ningru 36# (phenylethylphenol formaldehyde resin polyoxyethylene ether), A mixture of one or more of the following: resin polyoxyethyl ether), Nongru 1600# (phenylethylphenol polyoxyethyl polypropylene ether), ethylene oxide-propylene oxide block copolymer, OP series (nonylphenol polyoxyethylene ether), BY series (castor oil polyoxyethylene ether), Nongru 33# (alkyl aromatic polyoxyethylene polyoxypropylene ether), Span series (sorbitan monostearate), Tween series (dehydrated sorbitan fatty acid ester polyoxyethylene ether) or AEO series (fatty alcohol polyoxyethylene ether).

[0019] The dispersant is selected from a mixture of one or more of polycarboxylates, lignin sulfonates, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, alkylbenzene sulfonate calcium, naphthalenesulfonic acid formaldehyde condensate sodium salts, alkylphenol polyoxyethylene ethers, fatty amine polyoxyethylene ethers, fatty acid polyoxyethylene ethers or glycerol fatty acid ester polyoxyethylene ethers.

[0020] The wetting agent is selected from a mixture of one or more of sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, lakai powder BX, wetting and penetrating agent F, saponin powder, silkworm feces or soapberry powder.

[0021] The penetrant is selected from a mixture of one or more of penetrant JFC (fatty alcohol polyoxyethylene ether), penetrant T (diisooctyl maleate sulfonate), azone or organosilicon.

[0022] The antifreeze agent is selected from a mixture of one or more of ethylene glycol, propylene glycol, glycerol or urea.

[0023] The thickener is selected from a mixture of one or more of gelatin, sodium carboxymethyl cellulose, hydroxyethyl cellulose, sodium polyacrylate, modified starch, xanthan gum, bentonite, silicon dioxide or magnesium aluminum silicate.

[0024] The stabilizer is selected from a mixture of one or more of epoxy soybean oil, epichlorohydrin, BHT, ethyl acetate or triphenyl phosphate.

[0025] The disintegrant is selected from a mixture of one or more of bentonite, urea, ammonium sulfate, aluminum chloride, low-substituted hydroxypropyl cellulose, lactose, citric acid, succinic acid or sodium bicarbonate.

[0026] The defoaming agent is selected from a mixture of one or more of silicone oil, silicone compounds, C10-C20 saturated fatty acid compounds or C8-C10 fatty alcohol compounds.

[0027] The warning color is selected from pigments or dyes, including natural or synthetic ones, and is selected from a mixture of one or more of scarlet, rose red, fruit green, malachite green, alizarin red, phthalocyanine blue, titanium white, lead chrome yellow, iron blue, etc.

[0028] The film-forming agent is selected from a mixture of one or more of a protein film-forming agent, an acrylic resin film-forming agent, a butadiene resin film-forming agent, a polyurethane film-forming agent, a nitrocellulose film-forming agent, an acrylic resin modified casein film-forming agent, an acrylic resin polyurethane copolymer resin, polyethylene, polyethylene glycol, an acrylate modified butadiene resin, a polyurethane modified nitrocellulose film-forming agent, and the like.

[0029] The above-mentioned substances can all be purchased from the market.

[0030] The present invention also provides an agricultural compound nematicide, which is prepared by using the nematicide composition described in any one of the above technical solutions.

[0031] Preferably, the agricultural compound nematicide can be processed into any pesticide acceptable dosage form as required, wherein the preferred dosage forms are wettable powder, water dispersible granules, granules, suspensions, suspoemulsions, microemulsions, water emulsions, dispersible oil suspensions, seed treatment dispersible powders, and seed treatment suspensions.

[0032] The composition comprises the following components and contents when it is made into a wettable powder:

[0033] Dispira 0.01%-80%, oxazolidinone 0.01%-80%, wetting agent 0.01%-8%, dispersant 0.01%-12%, functional additive 0.01-10%, and filler supplement 100%.

[0034] The composition comprises the following components and contents when it is made into a dispersible powder for seed treatment:

[0035] Diplococcus 0.01%-80%, oxazolidinone 0.01%-80%, wetting agent 0.01%-8%, dispersant 0.01%-12%, defoamer 0.01-10%, warning color 0.01-20%, film-forming agent 0.01-20%, and filler 100%.

[0036] The composition comprises the following components and contents when it is made into water dispersible granules:

[0037] Dispira 0.01%-80%, oxadipamide 0.01%-80%, wetting agent 0.01%-8%, dispersant 0.01%-12%, defoamer 0.01-1%, disintegrant 0.01-20%, binder 0-20%, and filler 100%.

[0038] The composition comprises the following components and contents when it is made into a suspension:

[0039] Diplococtonia 0.01%-50%, oxazolidinone 0.01%-50%, wetting agent 0.01%-10%, dispersant 0.01%-12%, defoamer 0.01-1%, thickener 0.01-5%, antifreeze 0.01-10%, pH adjuster 0.01-10%, water to make up to 100%.

[0040] The composition comprises the following components and contents when it is made into a seed treatment suspension:

[0041] Diplococtonia 0.01%-50%, oxadipamide 0.01%-50%, wetting agent 0.01%-10%, dispersant 0.01%-12%, defoamer 0.01-1%, thickener 0.01-5%, antifreeze agent 0.01-10%, pH adjuster 0.01-10%, warning color 0.01%-20%, film-forming agent 0.01%-20%, water supplemented to 100%.

[0042] The composition comprises the following components and contents when it is made into a suspoemulsion:

[0043] Dispira 0.01%-50%, oxadiazine 0.01%-50%, solvent 0.01%-20%, wetting agent 0.01%-10%, emulsifier 0.01%-20%, dispersant 0.01%-12%, defoamer 0.01-1%, thickener 0.01-5%, antifreeze 0.01-10%, pH adjuster 0.01-10%, water supplemented to 100%.

[0044] The composition comprises the following components when it is made into a soluble solution:

[0045] Dispira 0.01%-50%, oxazolidinone 0.01%-50%, solvent 0.01%-50%, emulsifier 0.01%-20%, wetting agent 0.01%-20%, defoamer 0.01-1%, antifreeze 0.01-10%, pH adjuster 0.01-10%, water supplemented to 100%.

[0046] The composition comprises the following components and contents when it is made into an aqueous emulsion:

[0047] Diplococtonia 0.01%-50%, oxazolidinone 0.01%-50%, solvent 1%-30%, emulsifier 0.01%-20%, thickener 0.01%-2%, defoamer 0.01-1%, antifreeze 0.01-10%, pH adjuster 0.01-10%, water to make up to 100%.

[0048] The composition comprises the following components and contents when it is made into a microemulsion:

[0049] Diplococtonia 0.01%-50%, oxadipamide 0.01%-50%, solvent 0.01%-50%, emulsifier 0.01%-20%, defoamer 0.01-1%, antifreeze 0.01-10%, pH adjuster 0.01-10%, water to make up to 100%.

[0050] The composition comprises the following components and contents when it is made into a dispersible oil suspension:

[0051] Dispira 0.01%-50%, oxazolidinone 0.01%-50%, dispersant 0.01%-5%, emulsifier 0.01%-30%, defoamer 0.01-1%, thickener 0.01-10%, pH adjuster 0.01-10%, solvent (continuous phase) is replenished to 100%.

[0052] The composition comprises the following components and contents when made into granules:

[0053] Dispira 0.01%-50%, oxadipamide 0.01%-50%, wetting agent 0%-5%, dispersant 0%-10%, emulsifier 0%-20%, solvent (or oil substances such as methyl oleate) 0-50%, pH adjuster 0.01-10%, and carrier supplement 100%.

[0054] The present invention also provides a use of the nematicidal composition according to any one of the above technical solutions in preventing and controlling nematode infection and development on crops.

[0055] Preferably, the crop is selected from at least one of vegetables, cotton, fruit trees, corn, tobacco, flowers, melons, and beans;

[0056] The nematode is selected from at least one of root-knot nematodes, piercing nematodes, burr nematodes, sword nematodes, ring nematodes, and cyst nematodes.

[0057] Preferably, the method is used to control the infection and development of root-knot nematodes on potatoes, tomatoes, peppers and cucumbers.

[0058] The main technical indicators of various formulations of the present invention all meet the requirements of relevant standards such as the "Specifications for the Development of Pesticide Registration Product Specifications".

[0059] Compared with the prior art, the advantages and positive effects of the present invention are:

[0060] The combination of two spiral worms and oxadipazole was used, and it was found that after the root-knot nematodes were treated, they began to move slowly, and soon showed symptoms after treatment, and became completely paralyzed and motionless in 1 hour. The effect is significantly better than two single-dose components, and the composition is slowly and evenly distributed in the rhizosphere soil layer in the soil, which can effectively and long-term protect the plant root system from nematode invasion. Through the composite combination of the two, it can be used to prevent and treat various nematodes such as root-knot nematodes, perforating nematodes, burr nematodes, sword nematodes, and ring nematodes, and it also has a good preventive effect on cyst nematodes. Because both active ingredients are systemic, soil application does not affect the prevention and treatment of various leaf diseases and root diseases and the promotion of root growth regulation, and can even prevent and treat nematodes on the ground. And it is suitable for a variety of drug application methods (drip irrigation, root irrigation, furrow application, soil mixing, etc.). The composite of the two can be described as sterilization, insecticide, growth promotion, multiple functions, and after technical transformation and production, it will significantly reduce the cost of medication, improve prevention effect, reduce the amount of medication, and reduce environmental pollution, and the value created for enterprises and farmers is very considerable.

[0061] The specific manifestations are:

[0062] 1. Utilize new product functions to effectively solve the common nematode resistance problem in agricultural production;

[0063] 2. The synergistic effect is obvious. It is not a simple superposition of the activities of each component, but has a significant synergistic effect;

[0064] 3. Strong control efficacy, long-lasting effect, and low resistance risk;

[0065] 4. Sterilization, insecticide, growth promotion, multiple functions, one drug for multiple treatments, reducing the number of drug use and drug cost, increasing farmers' income, reducing pesticide residues and environmental pollution;

[0066] 5. It is composed of active ingredients with different mechanisms of action, which complement each other, help to overcome and delay the development of pest resistance and are safe for crops. DETAILED DESCRIPTION

[0067] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0068] The combination of Diplococtonia solani and Carbendazim in different ratios was tested to test the control effect on southern root-knot nematode.

[0069] The test method is as follows:

[0070] The embodiment of the present invention adopts a method combining indoor toxicity determination and field test. First, the co-toxicity coefficient (CTC) of two drugs after compounding in a certain ratio is determined by indoor toxicity determination. CTC < 80 is antagonistic effect, 80 ≤ CTC ≤ 120 is additive effect, and CTC > 120 is synergistic effect. On this basis, field test is carried out.

[0071] Test method: During the test, the water suspension concentrate stock solution of each mixed agent was diluted into five series of concentrations and placed in beakers for use.

[0072] Cultivation of southern root-knot nematodes: Take potato roots that produce a large number of root knots, rinse them gently with water, use a dissecting needle to gently pick up the milky white egg sacs on the diseased roots, put them in a small culture dish with a diameter of 6 cm, add a small amount of sterile water, and incubate them in a constant temperature box at 25°C for 3-4 days. Refer to Xu Jinjun's method throughout the process, collect second-instar larvae and add sterile water to prepare a suspension of a certain concentration (about 1000 / mL) for use.

[0073] The 96-well plate method was used. A continuous sampler was used to add a nematode suspension to the plate wells. 30 microliters were added to each well. Then, different doses of the drug solution were added in order from low to high, with a total of 5 different concentration gradients. The solution was shaken and placed in a constant temperature incubator at 25°C. The humidity was maintained at 60-80% for observation. Three replicates were set for each treatment, and a clear water control was set at the same time. The nematodes that were stiff and motionless were dead worms, and the nematodes that were bent and twisted were alive worms. The dead worm rate was observed at 24h, 48h, and 72h. The mortality rate and the corrected mortality rate were calculated. Referring to the Finney probability value analysis method, the corrected mortality rate of the pest was converted into a probability value (y), and the treatment concentration (μg / ml) was converted into a logarithmic value (x). The data was processed using IBM SPSS Statistics statistical software to obtain a toxicity regression equation, and the LC50 value of each agent was calculated from this. If the control mortality rate is greater than 10%, it is considered an invalid test. The calculation formula is as follows:

[0074] Mortality rate (%) = {(number of live worms before medication - number of live worms after medication) / number of live worms before medication} × 100%

[0075] Corrected mortality rate (%) = {(mortality rate of treatment group - mortality rate of control group) / (100 - mortality rate of control group)} × 100%

[0076] The co-toxicity coefficient CTC was calculated according to the Sun Yunpei formula. The calculation formula is as follows (taking Dispirillum as the standard agent, its toxicity index is 100):

[0077] Toxicity index (TI) of oxazolidinone = {LC50 of Diplodia / LC50 of oxazolidinone}×100%

[0078] Actual toxicity index (ATI) of M = {LC50 of Dispira / LC50 of M}×100%

[0079] Theoretical toxicity index (TTI) of M = TI of Diplodia × P 二螺虫 + Effective TI×P of oxadipate 噁霉灵 Co-toxicity coefficient (CTC) = {ATI of M / TTI of M} × 100%

[0080] Where: M is a mixture of different proportions

[0081] P 二螺虫 is the proportion of two spiral worms in the composition

[0082] P 噁霉灵 is the proportion of oxadiazine in the composition

[0083] The results of toxicity determination are shown in Table 1, and the results of synergistic determination are shown in Table 2. Among them, CK1 is a single dose of dispirilla, and CK2 is a single dose of oxadiazine.

[0084] Table 1 The results of toxicity test of different ratios of dimethoate and oxazolidinone to potato root-knot nematodes

[0085]

[0086]

[0087] Table 2 The results of the synergistic effect test of different ratios of dimethoate and oxazolidinone on potato root-knot nematodes

[0088] Pharmacy Actual Observed Value ATI Theoretical value TTI CTC Two-worm: oxazolidinone = 10:1 1.199603062 1.1245454545 106.67 Two-worm: oxazolidinone = 8:1 1.488216673 1.1188888889 133.01 Two-worm: oxazolidinone = 5:1 1.68163752 1.1033333333 152.41 Two-worm: oxazolidinone = 3:1 1.557805596 1.0800000000 144.24 Two-worm: oxazolidinone = 2:1 1.969739292 1.0566666667 186.41 Two-worm: oxazolidinone = 1:1 1.837966985 1.0100000000 181.98 Two-worm: oxazolidinone = 1:2 1.939935809 0.9633333333 201.38 Two-worm: oxazolidinone = 1:3 2.239809423 0.9400000000 238.28 Two-worm: oxazolidinone = 1:5 2.587767584 0.9166666667 282.30 Two-worm: oxazolidinone = 1:8 2.24933546 0.9011111111 249.62 Two-worm: oxazolidinone = 1:10 1.013170498 0.8954545455 113.15

[0089] It can be seen from Table 1 and Table 2 that the co-toxicity coefficient of the combination of two spiral worms and oxazolidinone is greater than 120, showing a synergistic effect, and the synergistic effect is most obvious when the weight ratio is 1:2, 1:3:1:5:1:8. This shows that the use of a compound preparation of two spiral worms and oxazolidinone with a weight ratio of 1:0.1-10 to control potato root-knot nematodes is reasonable and feasible.

[0090] The above method was used to determine the control effect of root-knot nematodes on tomatoes, peppers and cucumbers, and the synergistic coefficients within different ratio ranges were calculated. It was concluded that the combination of Diplococephalus and Methacin showed a synergistic effect of 1:2, 1:3:1:5:1:8, with the most obvious synergistic effect.

[0091] The present invention is described in detail below with reference to the examples. The percentages in the examples are all weight percentages, but the present invention is not limited thereto, and the treatment dosages for field efficacy are all dosages of active ingredients.

[0092] Wettable powders are as follows:

[0093] Example 1: (40% dimethoate·oxamyl wettable powder) 1:3

[0094] Components and contents: Dispirillum: 10%; Methadone: 30%; Wetting agent sodium dodecyl sulfate: 5%; Dispersant calcium lignin sulfonate: 8%; Dispersant naphthalene sulfonate: 4%; Filler selected from white carbon black: 5%; Defoamer: 0.2%; Kaolin: balance.

[0095] Example 2: (70% dimethoate·oxamyl wettable powder) 1:6

[0096] Components and contents: Dispirillum: 10%; Methacin: 60%; Wetting agent sodium dodecylbenzene sulfonate: 3%; Dispersant calcium alkylbenzene sulfonate: 7%; Dispersant naphthalene sulfonate: 3%; Filler selected white carbon black: 5%; Defoamer: 0.2%; Light calcium carbonate: the balance.

[0097] Example 3: (11% dimethoate·oxamyl wettable powder) 1:0.1

[0098] Components and contents: Dispirilla: 10%; Methacin: 1%; wetting agent selected from BX: sodium dodecylbenzene sulfonate and wetting and penetrating agent F, with dosages of 1%, 1%, and 1% respectively; dispersant selected from polycarboxylate and naphthalene sulfonate, with dosages of 1% and 3% respectively; white carbon black: 3%; defoamer: 0.2%; filler selected from diatomaceous earth: 20%, kaolin: balance.

[0099] The preparation method of the above-mentioned embodiments 1 to 3 is as follows: according to the components and their weight percentages provided in the embodiments, the active ingredients of oxadipamide and dispiril are added to the filler, and auxiliary agents such as a wetting agent and a dispersant are added thereto, mixed evenly, and then mixed again after air flow pulverization to obtain a wettable powder. The main equipment includes a mixer and an air flow pulverizer.

[0100] In the above examples, all indicators of the preparation samples meet the requirements of the wettable powder management specification.

[0101] The water dispersible granules are as follows:

[0102] Example 4: (30% dimethoate·oxamyl water dispersible granules) 1:2

[0103] Components and contents: Dispirillum: 10%; Methacin: 20%; Wetting agent sodium dodecylbenzenesulfonate: 3%; Dispersant alkylnaphthalenesulfonate formaldehyde condensate: 8%; Defoaming agent: 0.2%; Filler attapulgite: balance.

[0104] Example 5: (42% dimethoate·oxamyl water dispersible granules) 1:5

[0105] Components and contents: Dispirillum: 7%; Methacryloyl: 35%; Wetting agent saponin powder: 3%; Dispersant naphthalenesulfonic acid formaldehyde condensate sodium salt: 2%; Dispersant polycarboxylate: 2%; Defoamer: 0.2%; Filler attapulgite, the balance.

[0106] The preparation method of the above-mentioned embodiments 4 to 5 is as follows: according to the components and their weight percentages provided in the embodiments, the active ingredients of oxadipamide and dispirol are added to the filler, and auxiliary agents such as a wetting agent and a dispersant are added thereto, mixed, and after air flow pulverization, 10 to 25% of water is added, and then the water-dispersible granule product is obtained by kneading, granulating, drying, and sieving; or the pulverized powder is sprayed with water, granulated, dried, and then sieved in a boiling granulator to obtain the product. The main equipment includes a mixer: an air flow pulverizer, a kneader, an extrusion granulator, a drying room or a fluidized bed dryer, or a boiling granulator, and a sieving machine.

[0107] The above examples and preparation samples all meet the requirements of the water dispersible granules management standard.

[0108] The suspension is as follows:

[0109] Example 6: (27% dimethoate·oxamyl suspension) 1:8

[0110] Components and contents: Dispirilla: 3%; Methacin: 24%; Wetting agent NP-10: 2%; Dispersant polycarboxylate: 2%; Dispersant phosphate: 2%; Thickeners are xanthan gum and aluminum magnesium silicate, with dosages of 0.1% and 1% respectively; Antifreeze agent ethylene glycol: 5%; Defoaming agent silicone compound: 0.2%; Water, the balance.

[0111] Example 7: (5.5% dimethoate·oxamyl suspension) 1:10

[0112] Components and contents: Dispirilla: 0.5%; Methacin: 5%; Wetting agent NP-10: 1%; Dispersant alkylphenol polyoxyethylene ether: 3%; Dispersant polycarboxylate: 2%; Xanthan gum and aluminum magnesium silicate are used as thickeners, with dosages of 0.15% and 2% respectively; Antifreeze agent ethylene glycol: 5%; Defoaming agent silicone oil: 0.2%; Water: balance.

[0113] Comparative Example 1: (30% oxazolidinone suspension)

[0114] Components and contents: 30% of oxadipate; 2% of sodium lauryl sulfate as wetting agent; 2% of polycarboxylate as dispersant; 3% of phosphate ester as dispersant; 2% of alkylphenol polyoxyethylene ether as dispersant; xanthan gum and aluminum magnesium silicate as thickeners, with dosages of 0.15% and 1.5% respectively; 5% of ethylene glycol as antifreeze agent; 0.2% of silicone oil as defoaming agent; and the balance of water.

[0115] Comparative Example 2: (30% dispiril suspension)

[0116] Components and contents: Dispiril: 30%; wetting agent sodium dodecyl sulfate: 2%; dispersant polycarboxylate: 2%; dispersant phosphate: 3%; dispersant macromolecular polymer: 2%; thickeners are xanthan gum and aluminum magnesium silicate, with dosages of 0.1% and 1% respectively; antifreeze agent ethylene glycol: 5%; defoaming agent silicone oil: 0.2%; water: balance.

[0117] The preparation methods of the above-mentioned Examples 6 to 7 and Comparative Examples 1 and 2 are as follows: according to the components and weight percentages provided in the examples, the active ingredient oxazolidinone and the auxiliary agents such as dispiracil, wetting agent, dispersant, thickener, antifreeze agent, defoamer, etc. are placed in a reaction kettle in sequence, water is added to mix evenly, high-speed shearing, wet sand milling, and finally homogenization filtration are performed to obtain the product. The main equipment is a batching kettle, a colloid mill or a homogenizing mixer, and a sand mill.

[0118] All indicators of the above examples and comparative examples meet the requirements of the suspension management standards.

[0119] The dispersible oil suspensions are as follows:

[0120] Example 8: (30% dimethoate·oxamyl dispersible oil suspension) 1:2

[0121] Components and contents: Dispirilla: 10%, Methadone: 10%, calcium salt of dodecylbenzenesulfonate: 5%, castor oil polyoxyethylene ether: 1%, fatty alcohol polyoxyethylene ether: 8%, organic clay and fumed silica as thickeners, 0.7% respectively; soybean oil as the balance.

[0122] Example 9: (35% dimethoate·oxamyl dispersible oil suspension) 2:3

[0123] Components and contents: Dispirilla: 15%, Methadone: 20%, calcium salt of dodecylbenzenesulfonate: 4%, polyoxyethylene castor oil ether: 2%, polyoxyethylene fatty alcohol ether: 10%, isomeric alcohol ether: 2%, organic clay and fumed silica as thickeners, 0.6% respectively; soybean oil as the balance.

[0124] The preparation method of the above-mentioned embodiments 8 to 9 is as follows: according to the components and their weight percentages provided in the embodiments, soybean oil, active ingredient oxadipamide and dispiril, emulsifier, dispersant, thickener, defoamer and other auxiliary agents are placed in a reaction kettle in sequence, mixed evenly, subjected to high-speed shearing, wet sand milling, and finally homogenized filtration to obtain the product. The main equipment is a batching kettle, a colloid mill or a homogenizing mixer, and a sand mill.

[0125] In the above examples, all indicators of the preparation samples meet the requirements of the management specifications for dispersible oil suspensions.

[0126] The following uses the agents of Examples 1, 4, 5, 6, and 8 and the agents of Comparative Examples 1 and 2 to conduct field drip irrigation tests on potato root-knot nematodes at the dilution multiples shown in Table 3 to more comprehensively and accurately illustrate the effect of the present invention. The test site is selected from the test field of Qingdao Hansheng Biotechnology Co., Ltd. in Laixi City, Qingdao. During the test, the soil conditions, water and fertilizer, and various managements are consistent. The test results are shown in Table 3 below. Among them: Example 1: 40% two spiral worms·oxamyl wettable powder (10+30); Example 4: 30% two spiral worms·oxamyl water dispersible granules (10+20); Example 5: 42% two spiral worms·oxamyl water dispersible granules (7+35); Example 6: 27% two spiral worms·oxamyl water suspension (3+24); Example 8: 30% two spiral worms·oxamyl dispersible oil suspension (10+20); Comparative Example 1: 30% oxamyl water suspension, Comparative Example 2: 30% two spiral worm water suspension.

[0127] In this test, there are 8 treatments including Examples 1, 4, 5, 6, and 8: Comparative Examples 1 and 2 and no treatment, each treatment is repeated 3 times, 24 treatment plots in total, each plot is 30 square meters, isolation rows are set between plots, and each plot treatment is randomly arranged. The dosage of active ingredient per plant is 0.01g, the diluent is 400ml, and drip irrigation is implemented at the early stage of potato root knot nematode disease. Three points are randomly selected from each treatment plot, and three potatoes are selected from each point. The root knot condition of the potato roots is investigated before medication, 1 day, 3 days, and 7 days after medication. The grading standard of root knot nematode disease is: Grade 0 (no visible root knots in the root system), Grade 1 (1%-10% root knots), Grade 2 (10%-25% root knots), Grade 3 (25%-50% root knots), Grade 4 (50%-80% root knots), and Grade 5 (more than 80% root knots). The ability to control root-knot nematodes is based on two indicators: the root-knot nematode disease index and the control effect. At the same time, the growth of potatoes in each plot is observed and recorded: the occurrence of pesticide damage.

[0128] Calculation method of disease index and control effect:

[0129] Disease index (%) = {ΣNumber of diseased plants at each disease level × Number of disease levels / Total number of plants surveyed × Number of highest levels} × 100%

[0130] Control effect (%) = {(control disease index - treatment disease index) / control disease index} × 100%

[0131] Table 3 Control effect of different treatments on potato root knot nematode disease

[0132]

[0133]

[0134] It can be seen from the test results in Table 3 that after 1 day, 3 days and 7 days of application, the control effects of the agents in Example 1, Example 4, Example 5, Example 6 and Example 8 on potato root-knot nematodes are significantly higher than those of the single agent of dispirilla and oxazolidinone in Comparative Examples 1-2. The agent has a long lasting effect and still achieves a good control effect 7 days after application. Table 3 also shows that for preparations with the same ratio and content, the efficacy of the dispersible oil suspension is higher than that of the water-dispersible granules, which verifies that the oil in the preparation promotes the efficacy of the preparation. During the experiment, it was observed that the agents in each embodiment had no phytotoxicity to potatoes and had good safety.

[0135] In addition, referring to the above treatment method, 30% dimethoate·oxazolidinone OD (1:2) was used to conduct bioassays on three crops: tomato, pepper and cucumber. The dosage of the active ingredient per plant was 0.01g, and 400ml of the dilution was used for drip irrigation. The test data are shown in Table 4.

[0136] Table 4 Control effect of 30% dimethomorph·oxazolidinone OD on root-knot nematode disease in tomatoes, peppers and cucumbers

[0137]

[0138] Table 4 further verifies that the combination of Dispiracil and Methacin has a good control effect on root-knot nematodes in different crops, such as tomatoes, peppers, and cucumbers. The highest control effect is on the 3rd day, and the control ability is still high on the 7th day. The effective period is long. During the period of medication, crop production is normal and no obvious phytotoxicity occurs. Continue to use this agent, for cucumber root-knot nematodes, use 0.01g, 0.012g, and 0.015g of the active ingredient, respectively, and drip irrigation of 400ml of dilution to further verify the control effect of different dosages on root-knot nematodes. The test data are shown in Table 5.

[0139] Table 5 Effect of different dosages of 30% dimethoate·oxamexyl OD on the control of cucumber root-knot nematode disease

[0140]

[0141] It can be seen from Table 5 that with the increase of the dosage, the control effect of 30% dimethoate·oxazolidinone OD on cucumber root-knot nematode disease also showed an increasing trend, reaching a peak on the 3rd day, and still had a high control effect on the 7th day, with a long lasting effect. During the entire medication period, the cucumbers grew normally without obvious drug damage.

[0142] All of the above results fully demonstrate that the combination of Diplococtonia solani and Methacin has the advantages of significant synergistic effect and prolonged efficacy against root-knot nematodes, and is safe throughout the process.

Claims

1. A pesticide composition for controlling root-knot nematodes, characterized in that: The effective components are prepared by compounding dispiracil and oxazolidinone, and the weight ratio of the two is 1:80 to 80:1, preferably 1:20 to 20:

1.

2. The insecticide composition according to claim 1, characterized in that The weight percentage of the effective ingredients, dispiracil and oxazolidinone, in the insecticide composition is 1% to 90%, preferably 10% to 60%.

3. The insecticide composition according to claim 1 or 2, characterized in that: The insecticide composition also includes auxiliary ingredients of pesticide preparation, and the auxiliary ingredients of pesticide preparation include carriers, solvents, fillers and adjuvants.

4. The insecticide composition according to claim 3, characterized in that The carrier is selected from at least one of clay particles, broken brick particles, sand, plant-derived carriers, and fertilizers; The solvent is selected from at least one of organic solvents such as solvent oil, nitrogen methyl pyrrolidone, acetophenone, cyclohexanone, dimethyl sulfoxide, and plant-derived environmentally friendly solvents such as turpentine, methyl oleate, soybean oil, corn oil, and rapeseed oil; The filler is selected from at least one of kaolin, diatomaceous earth, bentonite, attapulgite, white carbon black, starch, and light calcium carbonate; The auxiliary agent includes at least one surfactant, and the surfactant is selected from at least one of an emulsifier, a dispersant, a wetting agent, and a penetrant.

5. The insecticide composition according to claim 4, characterized in that The auxiliary agent also includes other functional auxiliary agents, which are selected from at least one of antifreeze agents, thickeners, stabilizers, disintegrants or defoamers, warning colors, and film-forming agents.

6. An agricultural compound nematicide, characterized in that: The insecticide composition is prepared by using the insecticide composition described in any one of claims 1 to 5.

7. The agricultural compound nematicide according to claim 6, characterized in that: The dosage form is selected from at least one of wettable powder, water-dispersible granules, dry suspension, dispersible oil suspension, seed treatment suspension, seed treatment dispersible powder, soluble solution, emulsifiable concentrate, water suspension, aqueous emulsion, microemulsion, suspoemulsion and granules.

8. Use of the insecticide composition according to any one of claims 1 to 5 in preventing and controlling nematode infection and development on crops.

9. The use according to claim 8, characterized in that: The crop is selected from at least one of vegetables, cotton, fruit trees, corn, tobacco, flowers, melons, and beans; The nematode is selected from at least one of root-knot nematodes, piercing nematodes, burr nematodes, sword nematodes, ring nematodes, and cyst nematodes.

10. The use according to claim 8, characterized in that: Prevent and control the infection and development of root-knot nematodes on potatoes, tomatoes, peppers and cucumbers.

Images