Methods and uses for inhibiting preterm birth or shortening labor in sows

Abstract

The application provides a method and application for inhibiting premature birth of sows or shortening delivery process, and particularly discloses a method and application for inhibiting premature birth of sows or shortening delivery process of sows by using vitamin B6 or derivatives thereof. The application aims at the perinatal period problem of low survival rate of newborn piglets caused by premature birth or long delivery process of sows, and vitamin B6 or derivatives thereof is applied to sows in the late gestation period, and the result shows that the vitamin B6 or derivatives thereof can effectively inhibit premature birth of sows, shorten delivery process of sows and improve the survival rate of piglets, and is an effective method for improving delivery of sows and improving survival of piglets, and provides great value for promoting batch production of sows and high-efficiency breeding, and has important significance for solving the perinatal period premature birth problem in the field of reproduction.

Description

TECHNICAL FIELD

[0001] The present application mainly relates to the field of animal perinatal reproduction regulation, and particularly relates to a method for inhibiting sows from preterm birth or shortening sow labor and application. The method is based on the continuous administration of vitamin B6 in the late pregnancy of sows to inhibit sows from preterm birth, shorten sow labor, and improve the survival rate of piglets, which provides great value for promoting batch production of sows in China, and has important significance for solving the problem of perinatal preterm birth in the field of reproduction. BACKGROUND

[0002] The batch production technology of sows is a kind of high-efficiency controllable management technology system for improving the reproductive performance of sow herd in pig farms, which is based on the grouping of sow herd according to the scale and production plan, the replenishment of replacement sows according to the production plan, and the use of biological technology to achieve the purpose of synchronizing estrus, mating and parturition of replacement sows and multiparous sows in the same batch. However, in actual production, due to individual differences of sows, some sows may have preterm birth, resulting in low survival rate of piglets and difficulty in achieving the goal of synchronizing parturition. In addition, after the onset of parturition, the uterus contracts rhythmically, and the longer the time for the born piglets to stay in the sow's uterus or birth canal, the higher the risk of umbilical cord rupture, and the greater the risk of piglet death. Especially in the later stage of sow parturition, the labor is insufficient, the uterine contraction is weak, the piglets with umbilical cord rupture cannot be delivered in time, and the risk of asphyxiation death increases. Therefore, it is necessary to provide an effective solution to the above-mentioned problems of sows in the perinatal period.

[0003] Some existing parturition regulation technologies can induce the onset of parturition, but have no significant effect on sow labor. Although the combined administration of some drugs can induce sows to deliver in a concentrated manner, it may lead to prolonged labor, increased number of dead fetuses, and decreased number of live-born piglets and live piglet rate. The existing parturition regulation technology is applied to sows that are about to deliver, but has no application space for sows that have preterm birth. For the problem of preterm birth of sows, the method currently used in production is to feed sows with allyl estradiol, but feeding sows with allyl estradiol often leads to late birth of sows, prolonged labor, and high price of allyl estradiol. In view of the above reasons, the pig breeding industry urgently needs a simple, efficient and inexpensive parturition regulation scheme to inhibit preterm birth of sows and shorten sow labor.

[0004] Vitamin B6 is a water-soluble B vitamin, which exists in the form of pyridoxine, pyridoxal, pyridoxamine, pyridoxine phosphate, pyridoxal phosphate and pyridoxamine phosphate in the body, participates in key biochemical processes such as amino acid metabolism and neurotransmitter synthesis, has physiological functions such as anti-inflammatory, antioxidant, nerve regulation and anti-tumor, and is essential for maintaining the health of the nervous system, skin and immune function. However, up to now, there has been no relevant report or suggestion that vitamin B6 is used to inhibit preterm birth of sows, shorten sow labor, and improve the survival rate of piglets. SUMMARY

[0005] This invention, through extensive and creative experiments, compared the effects of administering vitamin B6 to sows in batches with natural parturition. The results showed that administering vitamin B6 in late gestation effectively inhibits premature birth, shortens the farrowing process, and increases piglet survival rate. This establishes a method for improving farrowing outcomes in batch sows, providing significant value for the promotion and application of batch sow production. Specifically, this invention includes the following:

[0006] A first aspect of the present invention provides a method for inhibiting premature birth in sows, shortening their labor process, and / or improving piglet survival rates, comprising the step of administering vitamin B6 or a derivative thereof to the sow.

[0007] In some embodiments, the method according to the present invention for inhibiting preterm birth in sows, shortening their labor process, and / or improving piglet survival rates includes the following steps:

[0008] (1) Sows were bred at the same time, and then pregnancy was checked to confirm pregnancy, and then they were transferred to the farrowing house;

[0009] (2) Administering vitamin B6 or its derivatives to sows during late gestation, wherein the daily dose of said vitamin B6 or its derivatives is in the range of 1-20 mg / kg.BW.

[0010] In some embodiments, the method according to the invention for inhibiting premature birth in sows, shortening their farrowing process, and / or improving piglet survival rates, wherein the daily dosage of vitamin B6 or its derivatives is in the range of 1-20 mg / kg.BW.

[0011] In some embodiments, the method according to the invention for inhibiting premature birth in sows, shortening their labor process, and / or improving piglet survival rates, wherein the sow is given vitamin B6 or a derivative thereof daily for 1-30 days prior to farrowing.

[0012] In some embodiments, the method according to the invention for inhibiting premature birth in sows, shortening their labor process, and / or improving piglet survival rates, wherein the sow is given vitamin B6 or a derivative thereof daily for 1-14 days prior to farrowing.

[0013] In some embodiments, the method of inhibiting premature birth in sows, shortening their labor process, and / or improving piglet survival rate according to the present invention is administered orally or by injection.

[0014] In some embodiments, the method according to the invention for inhibiting premature birth in sows, shortening their labor process, and / or improving piglet survival rates, wherein vitamin B6 or its derivatives are administered daily for 84-114 days of gestation.

[0015] In a second aspect, the present application provides a use of vitamin B6 or its derivative or the method or composition of the present application in the regulation of animal perinatal reproduction, wherein the use comprises inhibiting the premature birth of a sow, shortening the delivery process of a sow and / or improving the survival rate of piglets of a sow.

[0016] The present application is directed to the perinatal problem of low survival rate of newborn piglets caused by premature birth or long delivery process of sows, and the present application provides a method for improving the delivery of sows and improving the survival rate of piglets by administering vitamin B6 to sows in the late gestation period, which can effectively inhibit the premature birth of sows, shorten the delivery process of sows and improve the survival rate of piglets, and the method is an effective method for improving the delivery of sows and improving the survival rate of piglets, and can provide great value for promoting batch production of sows and efficient breeding, and has important significance for solving the problem of premature birth in the perinatal period in the field of reproduction. DETAILED DESCRIPTION

[0017] The various exemplary embodiments of the present application will now be described in detail, which should not be considered as limiting the present application, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present application.

[0018] It should be understood that the terms described in the present application are only for describing the specific embodiments, and are not used to limit the present application. In addition, for the numerical range in the present application, it should be understood that the upper limit and the lower limit of the range and every intermediate value between them are specifically disclosed. Every smaller range between the upper limit and the lower limit of these smaller ranges and between any stated value or intermediate value in the range is also included in the present application. The upper limit and the lower limit of these smaller ranges can be independently included or excluded from the range.

[0019] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application, the preferred methods and materials are described. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials in connection with which the documents are concerned. In the case of conflict, the content of this specification will control.

[0020] The present application provides a nutrient, a composition and a method for inhibiting the premature birth of a sow, shortening the delivery process and / or improving the survival rate of piglets, which comprises or consists of vitamin B6 or its derivative, and the present application also provides the use of vitamin B6 or its derivative for inhibiting the premature birth of a sow, shortening the delivery process of a sow and improving the survival rate of piglets.

[0021] In the present invention, "vitamin B6" refers to vitamin B6 group compounds, which include but are not limited to pyridoxine, pyridoxal, pyridoxamine and their phosphate esters, i.e. pyridoxine phosphate, pyridoxal phosphate, pyridoxamine phosphate. "Derivatives of vitamin B6" refer to acetal, ketal, organic acid ester, sulfonic acid ester, aminosulfonic acid ester, aminophosphonic acid ester, hemiacetal and hemiketal compounds and the like of the above-mentioned vitamin B6 group compounds.

[0022] In a preferred embodiment, the method for inhibiting premature birth or shortening the labor process of sows comprises the following steps:

[0023] (1) Sows are synchronized for mating, and then pregnancy is checked to confirm pregnancy, and sows in the late gestation period are transferred to a delivery house;

[0024] (2) Vitamin B6 or its derivatives are administered to sows in the late gestation period, and the daily dosage of vitamin B6 or its derivatives is in the range of 1-20 mg / Kg.BW, to inhibit premature birth, shorten the labor process of sows, and improve the survival rate of piglets.

[0025] In a preferred embodiment, the method for inhibiting premature birth, shortening the labor process of sows, and improving the survival rate of piglets using vitamin B6 is to continuously administer vitamin B6 or its derivatives every day from 84-114 days of pregnancy, preferably 84-113 days, further preferably 100-113 days, for example 100 days, 101 days, 102 days, 103 days, 104 days, 105 days, 106 days, 107 days, 108 days, 109 days, 110 days, 111 days, 112 days, 113 days, or any range within the above-mentioned days.

[0026] In a preferred embodiment, the daily dosage of vitamin B6 or its derivatives can vary in the range of 1-20 mg / Kg.BW, and the preferred dosage is 2-20 mg / Kg.BW per day, for example 2, 4, 6, 8, 10, 12, 14, 16, 18, 20 mg / Kg.BW, or any range within the above-mentioned dosage.

[0027] In a preferred embodiment, vitamin B6 or its derivatives are administered orally or by injection, preferably by injection. Those skilled in the art can understand that when oral administration is performed, any suitable oral dosage form of vitamin B6 or its derivatives can be selected, such as soft capsules, tablets and the like of vitamin B6 or its derivatives. When injection administration is performed, any suitable liquid dosage form of vitamin B6 or its derivatives can be selected, such as injections of vitamin B6 or its derivatives.

[0028] In a preferred embodiment, the sows are administered vitamin B6 or a derivative thereof daily during the 1-30 days prior to parturition, preferably during the 1-14 days prior to parturition, for example 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 days.

[0029] The present application further provides a composition comprising vitamin B6 or a derivative thereof for inhibiting preterm birth, shortening labor, and / or increasing survival rate of piglets in sows, which can further comprise optional additional substances, which are not particularly limited and can be selected as needed, which can or can not be any suitable agent for inhibiting preterm birth, shortening labor, and / or increasing survival rate of piglets in sows.

[0030] In the present application, the additional substances include but are not limited to enzymes, proteins, anti-nutritional factors, polysaccharides or fibers, etc.

[0031] In the present application, the composition comprises optional additives or excipients, such as additives or excipients used in feed production, examples of which include but are not limited to minerals, preservatives, antibiotics, fermentation products, fats, other essential ingredients, colorants, fillers, etc. The substances that can be added include marine products, dibasic calcium phosphate, calcium carbonate, calcium bicarbonate, magnesium oxide, multivitamins, minerals or other types of minerals or trace elements, essential amino acids such as lysine, methionine, tryptophan, threonine, and derivatives thereof, or combinations thereof.

[0032] Examples

[0033] The following shows the application of vitamin B6 in batch production of sows for inhibiting preterm birth, shortening labor in sows.

[0034] (1) Experimental methods and grouping

[0035] The batch-produced white sows were randomly divided into a natural parturition group and a vitamin B6 group, and the sows were multiparous sows with a body weight of 250-270 kg, and the amount of feed ration was 2.5-3 kg. The experimental sows in each group were fed according to the requirements of the farm management during the experiment, and free water was provided. The feed ration used can use the formula used during the gestation period of sows (for example, the formula recommended in "Batch Production Management Technology of Sows") or adjust the components as needed. The nutritional level of the feed ration used in this embodiment is shown in Table 1 below.

[0036] Table 1 Nutritional level of sows during the gestation period

[0037]

[0038] The specific grouping is as follows:

[0039] (a) Natural farrowing group: pregnant sows were not treated, and natural farrowing data were recorded.

[0040] (b) Vitamin B6-low dose group: sows were fed with 0.075 g (per sow) of vitamin B6 at 8:00 every day from 100 to 113 days of pregnancy until the sow's expected delivery date of 113 days.

[0041] (c) Vitamin B6-medium dose group: sows were fed with 0.75 g (per sow) of vitamin B6 at 8:00 every day from 100 to 113 days of pregnancy until the sow's expected delivery date of 113 days.

[0042] (d) Vitamin B6-high dose group: sows were fed with 7.5 g (per sow) of vitamin B6 at 8:00 every day from 100 to 113 days of pregnancy until the sow's expected delivery date of 113 days.

[0043] (2) Observation and recording of sow delivery

[0044] The sow's delivery date, time, duration, and farrowing data (including total number of piglets, number of live piglets, number of stillborn piglets, number of mummies, average delivery duration, and live piglet rate) were recorded.

[0045] (3) Statistical analysis

[0046] Single-factor variance analysis and chi-square test were performed using SPSS 22.0 software (SPSS GmbH, Munich, Germany). Single-factor variance analysis was used to analyze the average delivery duration, litter size, and number of live piglets, and the results were expressed as mean ± standard deviation. Chi-square test was used to analyze the preterm birth rate and live piglet rate. P<0.05 was considered statistically significant.

[0047] (4) Experimental results

[0048] In this example, compared with the control group (natural farrowing), feeding appropriate doses of vitamin B6 (medium dose group and high dose group) at the end of pregnancy in sows can significantly shorten the sow's delivery duration, reduce the number of stillborn piglets, and increase the sow's live piglet rate (Table 2). However, feeding a low dose of vitamin B6 had no obvious effect on the sow's delivery duration and preterm labor inhibition, and the live piglet rate showed an increasing trend, but there was no significant difference.

[0049] Table 2 Effect of different farrowing schemes on sow delivery duration and farrowing data

[0050]

[0051]

[0052] Note: Different lowercase letters on the right shoulder indicate that the indicators in the same row are significantly different, P<0.05.

[0053] Industrial value of the present application:

[0054] Since the outbreak of African swine fever in August 2018, the traditional continuous production mode has been difficult to adapt to the rapid development of the pig industry, and the entire pig market is facing severe challenges, sow batch production technology has become an important measure for transformation and upgrading, and the era of small-scale pig raising will become a thing of the past in the future, the emergence of large-scale and batch production methods will bring about earth-shaking changes to the pig industry. The problems of sow premature birth, long labor and low live piglet rate seriously affect the economic benefits of the pig farm. However, the present application is a method for improving the delivery effect of sows based on vitamin B6, which effectively improves the live piglet rate of batch sows, and using the present application can increase 70 live piglets per 100 sows, according to the current market price of 350-450 yuan per weaned piglet, using the present application will generate 2.45-3.15 million yuan of economic value per 100 sows, and the method has low cost, high efficiency and simple operation, which provides an effective method for cost reduction and efficiency improvement for the pig industry, therefore, the present application will produce important economic value and social value for promoting sow batch production.

[0055] The present application solves the problems of sow premature birth, long labor and low live piglet rate by additionally applying a specific dose of vitamin B6, which reduces the number of stillborn piglets by 0.48 per batch of sow delivery, effectively increases the number of live piglets per litter by 0.7, and according to the calculation of 2.23 litters per sow per year in China in 2024, and a weaned piglet survival rate of 95%, the PSY will increase by 0.71*2.23*95%=1.48, therefore, the present application is an effective way to improve the quality and efficiency of pig breeding, and the present application also has the advantages of simplicity, efficiency, strong innovation, broad industrial prospects and value.

[0056] Although the embodiments of the present application have been shown and described above, it should be understood that the above embodiments are exemplary and should not be construed as limiting the present application, and those skilled in the art can make changes, modifications, replacements and variations to the above embodiments within the scope of the present application.

Claims

1. Use of vitamin B6 or a derivative thereof for the preparation of a medicament for inhibiting premature birth or shortening of the delivery process in sows, characterized in that, The drug takes vitamin B6 or its derivative as the only active ingredient, and the inhibition of sows' premature birth or shortening of delivery process includes the following steps: (1) Sows are synchronized with mating, and then pregnancy is checked to confirm pregnancy, and then they are transferred to a delivery house; (2) The drug is administered to sows every day in the last period of pregnancy, which is 7-14 days before delivery, and the daily dosage of vitamin B6 or its derivative is in the range of 1-20 mg / Kg.BW.

2. Use of vitamin B6 or a derivative thereof according to claim 1 for the manufacture of a medicament for inhibiting premature birth or shortening of the delivery process in sows, characterized in that, The administration mode is oral.

3. Use of vitamin B6 or a derivative thereof according to claim 1 for the manufacture of a medicament for inhibiting premature birth or shortening of the delivery process in sows, characterized in that, The administration mode is injection.

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