Pen type optical detection device for qualitative and quantitative analysis of dry test paper

Abstract

The invention belongs to the technical field of rapid detection, and mainly relates to a pen-type optical detection device for qualitative and quantitative analysis of dry test paper, which comprises a test paper adapter, a lens-type optical detection pen and a control terminal in communication connection with the lens-type optical detection pen, the test paper adapter is used for fixing to-be-tested paper and comprises at least two detection areas, and the detection areas are used for detection of the lens type optical detection pen and shielding of ambient light; the lens type optical detection pen detects the to-be-tested paper, and the testing process is point-to-test; the control terminal is used for receiving detection data transmitted by the lens type optical detection pen; the optical detection device has the advantages of active locating, simple structure, high portability, remarkable cost effectiveness and the like, the point-and-test handheld locating design provides great flexibility and convenience, and the optical detection device is particularly suitable for application scenes of health inspection and POCT (point-of-care testing); in addition, the invention further designs a detection method for qualitative and quantitative analysis of the dry test paper, and the convenience and rapidity of detection are further improved.

Description

Technical Field

[0001] The invention belongs to the field of rapid testing technology, and in particular relates to a pen-type optical detection device for qualitative and quantitative analysis of dry test paper. Background Art

[0002] The speed and accuracy of testing are the two main development directions in the field of testing and analysis. In the fields of medical diagnosis, health management, food safety, environmental monitoring, etc., rapid testing technology plays an increasingly important role, and the demand for rapid and on-site testing of biological and chemical molecules has surged. In the field of clinical medicine, the development of rapid testing technology has brought many beneficial changes to the promotion of POCT and the early diagnosis and treatment of diseases; in the field of health management, individuals, families, and primary medical institutions need the support of rapid testing technology for monitoring biochemical indicators such as blood sugar, blood lipids, and uric acid and screening of disease biomarkers; in the food and health and epidemic prevention industry, production departments, technical supervision departments, product sales departments, and users need convenient and fast methods to monitor heavy metals, pesticide and veterinary drug residues and microorganisms; environmental monitoring law enforcement departments conduct on-site analysis of soil, air, and water, as well as military commanders and fighters' safety assessment of water, air, and food in the wild living environment and preliminary evaluation of their physical condition, all of which are inseparable from the rapid detection of biological and chemical molecules.

[0003] Dry analysis technology is an important part of rapid testing technology. It only needs to add the liquid sample directly to the reagent carrier that has been solidified in a special structure. The water in the sample is used as the solvent to dissolve the reagent solidified on the carrier, and then react with the components to be tested in the sample to achieve analytical determination of the concentration or activity of the test substance. This technology has both qualitative and quantitative functions, and mainly includes dry chemistry, immunocolloidal gold chromatography, immunofluorescence chromatography, etc. Most samples of dry analysis technology can be tested as soon as they are taken, which largely solves the problems of difficult storage, transportation, mixing and loss, and cross-contamination of samples; the operation is simple and convenient, so personnel can complete the measurement without special training.

[0004] However, if the dry analysis results are to be quantified, accurate signal analysis equipment is also required. The current quantitative equipment has a single function and is generally only suitable for passive limit analysis of one signal and a single test paper type. It is difficult to be compatible with different test paper products; it is also relatively large in size for on-site testing. The present invention provides an active positioning, compact, portable device that is commonly used for a variety of optical signals such as reflected light, fluorescence, and chemiluminescence. It cooperates with smart terminals, has a clear detection interface, convenient data management, and can also be uploaded to the cloud. The simplicity and accessibility of its operation are particularly suitable for large-scale on-site and personal and family self-testing, especially for infants and young children; it can also directly perform non-invasive blood oxygen, hemoglobin, jaundice and other tests on the skin. Therefore, application scenarios include the field, on-site, personal, family, and laboratory.

[0005] Based on this, there is an urgent need for a pen-type optical detection device and method for qualitative and quantitative analysis of dry test paper with strong compatibility, active positioning, and flexible operation. Summary of the invention

[0006] One of the purposes of the present invention is to provide a pen-type optical detection device for qualitative and quantitative analysis of dry test paper with strong compatibility, active positioning and flexible operation in view of the shortcomings of the prior art.

[0007] In order to achieve the above technical objectives, this application implements the following technical solutions:

[0008] A pen-type optical detection device for qualitative and quantitative analysis of dry test paper, comprising a test paper adapter, a lens-type optical detection pen and a control terminal connected to the lens-type optical detection pen for communication;

[0009] The test paper adapter is used to fix the test paper, and includes at least two detection areas, and the detection areas are used for the lens-type optical detection pen to detect and shield the ambient light;

[0010] The lens-type optical detection pen detects the test paper, and the test process is point-and-click.

[0011] The control terminal is used to receive the detection data transmitted by the lens-type optical detection pen.

[0012] The above technical solution produces the following technical effects:

[0013] The use of the lens-type optical detection pen of the present application makes the detection process more convenient and efficient. The lens-type optical detection pen can accurately detect specific areas on the test paper. At the same time, due to the design of the detection area, the ambient light is effectively shielded, ensuring the accuracy of the detection. In addition, the portability of the lens-type optical detection pen makes on-site detection possible without the need for complex equipment and professional operators, greatly reducing the cost and time of detection.

[0014] Furthermore, the introduction of the control terminal not only realizes the instant transmission of test data, but also provides a platform for data storage and analysis. Users can view the test results through the control terminal and conduct further data processing and analysis. The intelligent design of the control terminal makes the entire test process more intuitive and easy to operate, greatly improving the user experience.

[0015] As a further improvement of the present application's pen-type optical detection device for qualitative and quantitative analysis of dry test paper, the lens-type optical detection pen includes a photoelectric detection module, a light source module, and a probe assembly connected to the photoelectric detection module.

[0016] The light source module is used to provide light to illuminate the test paper through the probe assembly, and the photoelectric detection module is used to collect the light signal transmitted back to the probe assembly by the test paper and convert the light signal into an electrical signal. The light signal includes reflected light, fluorescence, and chemiluminescence signals.

[0017] As a further improvement of the pen-type optical detection device for qualitative and quantitative analysis of dry test paper in the present application, the probe assembly includes a probe light shield and a hemispherical lens arranged in the light shield. The light shield is provided with an annular gasket, which is used to clamp the hemispherical lens.

[0018] As a further improvement of the pen-type optical detection device for qualitative and quantitative analysis of dry test paper in the present application, the lens-type optical detection pen also includes a main control module, which receives the electrical signal transmitted by the photoelectric detection module, and the main control module is communicatively connected to the control terminal.

[0019] As a further improvement to the pen-type optical detection device for qualitative and quantitative analysis of dry test paper in the present application, the main control module includes a power management module, which is used to drive the main control module to drive the photoelectric detection module and the light source module to work.

[0020] As a further improvement of the pen-type optical detection device for qualitative and quantitative analysis of dry test strips in the present application, the photoelectric detection module includes a photoelectric sensor, a filter and an ADC converter. The photoelectric sensor is used to collect the light signal passing through the filter and convert the light signal into an electrical signal through the ADC converter.

[0021] As a further improvement to the pen-type optical detection device for qualitative and quantitative analysis of dry test paper of the present application, the photoelectric sensor is a multi-spectral array sensor or a miniature spectrometer or a miniature camera.

[0022] As a further improvement to the present invention's pen-type optical detection device for qualitative and quantitative analysis of dry test paper, the light source module is a ring-shaped light source composed of at least one single-wavelength LED or at least one full-spectrum white light LED;

[0023] Among them, single-wavelength LEDs and full-spectrum white light LEDs can be controlled separately, and the wavelength range of single-wavelength LEDs is: 365nm-620nm.

[0024] As a further improvement to the present application of a pen-type optical detection device for qualitative and quantitative analysis of dry test paper, the main control module includes an integrated control circuit board and a circuit board fixing member;

[0025] In addition, the lens-type optical detection pen also includes a shell and a cover plate covering the top of the shell, and the integrated control circuit board and the circuit board fixing parts are both arranged in the shell.

[0026] The second object of the present invention is to provide a detection method applicable to a variety of optical signals to address the deficiencies of the prior art, thereby enabling qualitative detection of different types of dry test papers.

[0027] In order to achieve the above technical objectives, this application implements the following technical solutions:

[0028] A detection method for qualitative and quantitative analysis of dry test strips, comprising a detection device for qualitative and quantitative analysis of dry test strips as described above, wherein a photoelectric acquisition module collects light intensity of at least one wavelength and outputs a corresponding electrical signal to a main control module, the main control module preprocesses the electrical signal, and calculates the optical density value of the preprocessed data signal; the main control module transmits the preprocessed electrical signal and the optical density value to a control terminal;

[0029] The preprocessing method is:

[0030]

[0031] Where I(n) represents the value of the electrical signal at time point n, is the preprocessed electrical signal, N represents the window length of the moving average of the above processing method, K is a variable used for indexing, and represents the offset of each data point used in the accumulation process;

[0032] The optical density value is calculated as follows:

[0033]

[0034] Among them, OD λ is the optical density value at wavelength λ, where is the light intensity value with wavelength λ after the above preprocessing, I incident，λ is the light source emitted by the light source module with a wavelength of λ.

[0035] The above technical solution produces the following technical effects:

[0036] This detection method achieves rapid and accurate detection of dry test strips by using a test strip adapter and a lens-type optical detection pen. The high-precision optical detection capability of the lens-type optical detection pen can be used to accurately detect specific areas on the test strip. At the same time, the design of the test strip adapter ensures that the ambient light during the detection process is effectively shielded, thereby ensuring the accuracy of the test results. In addition, this method also allows users to view the test results in real time through the control terminal and perform further data processing and analysis, making the entire detection process more intuitive and easy to operate. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0038] Figure 1 This is a schematic diagram of the structure of Example 1 of the present invention;

[0039] Figure 2 It is a schematic diagram of the structure of the probe assembly in Example 1 of the present invention;

[0040] Figure 3 This is a working flow chart of a pen-type optical detection device for qualitative and quantitative analysis of dry test paper in Example 1 of the present invention;

[0041] Figure 4 This is a working diagram of the main control module in Example 1 of the present invention;

[0042] Figure 5 It is the detection result of the lens-type optical detection pen provided in Example 2 of the present invention for the reflected light of wavelengths of 405nm, 450nm, 490nm, 540nm, 570nm, 620nm, 670nm and 365nm reflected by the standard grayscale card;

[0043] Attached Figure 6 is the quantitative dry chemical test result of uric acid level by the lens-type optical detection pen provided in Example 3 of the present invention;

[0044] Attached Figure 7 is the result of color recognition of the standard color card by the lens-type optical detection pen provided in Example 4 of the present invention;

[0045] Attached Figure 8 This is the detection result of the fluorescent signal of the fluorescent nano-microspheres by the lens-type optical detection pen provided in Example 5 of the present invention.

[0046] in:

[0047] 1-Test strip adapter;

[0048] 11-Detection area;

[0049] 2- Lens-type optical detection pen;

[0050] 21- Photoelectric detection module;

[0051] 22-light source module;

[0052] 23-probe assembly;

[0053] 231-probe light shield;

[0054] 2311-Ring washer;

[0055] 232- hemispherical lens;

[0056] 24- main control module;

[0057] 241-power management module;

[0058] 242-integrated control circuit board;

[0059] 243-circuit board fixing piece;

[0060] 25-housing;

[0061] 26-cover plate;

[0062] 3- Control terminal. DETAILED DESCRIPTION

[0063] The following will be combined with the drawings in the embodiments of the present application to clearly and completely describe the technical solutions in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in the field without making creative work are within the scope of protection of the present application. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of the present application. The terms used herein in the specification of the present application are only for the purpose of describing specific embodiments and are not intended to limit the present application.

[0064] In the description of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected" and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0065] Although the present application is disclosed as above with preferred embodiments, it is not intended to limit the claims. Any technical personnel in this field may make several possible changes and modifications without departing from the concept of the present application. Therefore, the scope of protection of the present application shall be based on the scope defined by the claims of the present application.

[0066] The present invention is further described in detail below in conjunction with specific embodiments, but the embodiments of the present invention are not limited thereto.

[0067] Example 1

[0068] like Figure 1-4As shown, in order to solve the technical defects of the rapid detection equipment used for qualitative and quantitative analysis of dry test strips in the prior art, which is single in function, generally only applicable to the analysis of one signal and used for on-site detection, and relatively large in size, this application designs a pen-type optical detection device for qualitative and quantitative analysis of dry test strips. The device is not only non-invasive, compact, and portable, but also applicable to the detection of multiple optical signals. At the same time, the use of the device in conjunction with the intelligent control terminal 3 makes the detection interface clearer, data management more convenient, and supports data upload to the cloud.

[0069] Specifically, the present application is a pen-type optical detection device for qualitative and quantitative analysis of dry test paper, including a test paper adapter 1, a lens-type optical detection pen 2, and a control terminal 3 that is communicatively connected to the lens-type optical detection pen 2; wherein, the test paper adapter 1 is used to fix the test paper, including at least two detection areas 11, and the detection area 11 is used for the lens-type optical detection pen 2 to detect and shield the ambient light; the lens-type optical detection pen 2 detects the test paper through the probe assembly 23, and the test process is point-to-point measurement; the control terminal 3 is used to receive the detection data transmitted by the lens-type optical detection pen 2. With this design, the portability and ease of use of the lens-type optical detection pen 2 greatly improve the efficiency and convenience of on-site detection. Users can easily carry the pen-type detection device to the site without carrying bulky equipment or waiting for professionals to arrive. In addition, the probe assembly 23 of the lens-type optical detection pen 2 is exquisitely designed and can be accurately aligned with the detection area 11 on the test paper, ensuring the accuracy of the detection.

[0070] It is worth noting that the lens-type optical detection pen 2 can independently complete the detection of dry test paper (test paper), and the detection method is point-to-point detection. Among them, the lens-type optical detection pen 2 is used to perform qualitative and quantitative analysis on the optical signal collected by the test paper.

[0071] Furthermore, the lens-type optical detection pen 2 includes a photoelectric detection module 21, a light source module 22, and a probe assembly 23 connected to the photoelectric detection module 21; the light source module 22 is used to provide light to illuminate the test paper through the probe assembly 23, and the photoelectric detection module 21 is used to collect the light signal of the light reflected back from the test paper to the probe assembly 23 and convert the light signal into an electrical signal. In addition, the lens-type optical detection pen 2 also includes a main control module 24, which receives the electrical signal transmitted by the photoelectric detection module 21, and the main control module 24 is connected to the control terminal 3 in communication. Among them, the photoelectric detection module 21 includes a photoelectric sensor, a filter and an ADC converter, and the photoelectric sensor is used to collect the light signal passing through the filter and convert the light signal into an electrical signal through the ADC converter.

[0072] Furthermore, the working principle of the above-mentioned pen-type optical detection device for qualitative and quantitative analysis of dry test strips is as follows: the user places the test paper in the detection area 11 of the test strip adapter 1, ensuring that the test strip is correctly fixed and the detection area 11 is exposed. Then, the user holds the lens-type optical detection pen 2 and aligns the probe assembly 23 with the detection area 11 of the test strip adapter 1. The light source module 22 of the lens-type optical detection pen 2 provides a stable light source, which is irradiated onto the test strip, while the photoelectric detection module 21 is responsible for collecting the reflected light signal. It should be noted here that if the signal detected by this application includes not only reflected light but also fluorescence and chemical light, etc., there is no need for the light source module 22 to emit a light source. This is only a description of the reflected light situation, but it does not mean that this application protects the reflected light situation.

[0073] These optical signals are collected by photoelectric sensors, and light of specific wavelengths is filtered through filters, and then converted into electrical signals through ADC converters. The electrical signals are then transmitted to the main control module 24, which processes these signals and further analyzes and processes the data through the integrated control circuit board 242. Finally, the main control module 24 transmits the processed data to the control terminal 3, and the user can view the detection results through the control terminal 3 and store and analyze the data.

[0074] Furthermore, the main control module 24 includes a power management module 241, and the power management module 241 is used to drive the main control module 24 to drive the photoelectric detection module 21 and the light source module 22 to work. In the specific implementation process, the main control module 24 includes an integrated control circuit board 242 and a circuit board fixing member 243, and a switch for starting the power management module 241 is set on the integrated control circuit board 242. In addition, the lens-type optical detection pen 2 also includes a shell 25 and a cover plate 26 covering the top of the shell 25. The integrated control circuit board 242 and the circuit board fixing member 243 are both arranged in the shell 25, and a button for pressing the switch is designed on the shell 25. Through the above design, the user can easily turn on or off the detection device, ensuring the portability and ease of use of the device. In addition, the design of the integrated control circuit board 242 enables the main control module 24 to perform CPU control, op amp driving and communication, and the power management module 241 is also integrated in the integrated control circuit board 242 to provide voltage-stabilized power and parameter control for the light source module 22 and the photoelectric detection module 21.

[0075] Furthermore, the photoelectric sensor can be a multi-spectral array sensor, a miniature spectrometer or a miniature camera, which enables the lens-type optical detection pen 2 to adapt to different types of dry test paper detection requirements. The photoelectric sensor is directly or indirectly connected to the probe assembly 23, and its detection signal includes any one of reflected light, fluorescence, and chemiluminescence.

[0076] Furthermore, the light source module 22 can be a ring light source composed of at least one single-wavelength LED or at least one full-spectrum white light LED. Both the single-wavelength LED and the full-spectrum white light LED can be controlled separately to provide light sources of different wavelengths to meet the detection requirements of different test strips. In addition, the wavelength range of the single-wavelength LED is: 365nm-620nm, which covers a wide range from ultraviolet to visible light, allowing the detection device to cope with a variety of different chemical reactions and material detection. Full-spectrum white light LEDs can provide a wider wavelength range, which is suitable for complex detection scenarios that require multi-wavelength light sources.

[0077] Furthermore, the control terminal 3 of the lens-type optical detection pen 2 is a computer, a smart phone or a smart tablet; the control terminal 3 has a built-in control program, which can realize functions such as equipment calibration, project selection, parameter setting, algorithm correction, result display, storage, statistics, etc. The control program can also be built into the lens-type optical detection pen 2 to complete the above-mentioned parameter setting, result display and other functions on the body of the lens-type optical detection pen 2.

[0078] Specifically, the test paper adapter 1 of the pen-type optical detection device contains slots for multiple test paper detection products and exposes the detection area 11, so that the detection probe of the lens-type optical detection pen 2 is tightly adapted to the test paper fixed therein, while accurately limiting and shielding the interference of ambient light, and realizing qualitative and quantitative analysis of the optical signals of the square, circular, and strip-shaped detection areas 11 of the test paper.

[0079] Furthermore, the probe assembly 23 includes a probe light shield 231 and a hemispherical lens 232 disposed in the light shield, and the light shield is provided with an annular gasket 2311, and the annular gasket 2311 is used to clamp the hemispherical lens 232. Among them, the probe light shield 231 is used to match and closely contact the detection area 11 exposed by the test paper adapter 1 to prevent interference from ambient light. In the specific implementation process, the position of the embedded hemispherical lens 232 needs to ensure that the detection window falls within 1-2 times the focal length of the lens. As a result, the hemispherical lens 232 can effectively collect the reflected light signal from the test paper, enhance the strength and clarity of the signal, and thus improve the sensitivity and accuracy of the detection.

[0080] Furthermore, the probe assembly 23 of the lens-type optical detection pen 2 also includes an adjustable light source intensity controller, allowing the user to adjust the brightness of the light source according to different detection environments and test paper types. This design enables the detection device to adapt to a variety of different detection conditions, whether indoors or outdoors, to obtain stable and reliable detection results.

[0081] Preferably, the lens-type optical detection pen 2 also has an automatic calibration function. Through the built-in calibration program, the user can regularly calibrate the detection device to ensure the accuracy and consistency of the detection data. The calibration process is simple and quick. The user only needs to follow the prompts of the control terminal 3 to complete the calibration without the intervention of professional technicians.

[0082] In summary, the pen-type optical detection device and method for qualitative and quantitative analysis of dry test paper provided by the present invention not only improves the accuracy and efficiency of detection, but also makes the entire detection process more intuitive and easy to operate through intelligent design, greatly improving the user experience.

[0083] In addition, the present application also provides a detection method applicable to a variety of optical signals, including a detection device for qualitative and quantitative analysis of dry test paper as described above, wherein the photoelectric acquisition module 21 collects the light intensity of at least one wavelength and outputs the corresponding electrical signal to the main control module 24, the main control module 24 preprocesses the electrical signal, and calculates the optical density value of the preprocessed data signal; the main control module 24 transmits the preprocessed electrical signal and the optical density value to the control terminal 3;

[0084] The preprocessing method is:

[0085]

[0086] Where I(n) represents the value of the electrical signal at time point n, is the preprocessed electrical signal, N represents the window length of the moving average of the above processing method, K is a variable used for indexing, and represents the offset of each data point used in the accumulation process;

[0087] In the above detection process, if the electrical signal data stream includes a light intensity data stream with a wavelength of λ of 450nm: 450 : [75, 74, 76, 73, 77], the data after preprocessing and smoothing is: [75, 74.33, 75.33, 75].

[0088] The optical density value is calculated as follows:

[0089]

[0090] Among them, OD λ is the optical density value at wavelength λ, where is the light intensity value with wavelength λ after the above preprocessing, I incident，λ is the light source emitted by the light source module 22 with a wavelength of λ.

[0091] Specifically, the above-mentioned light intensity preprocessing electrical signal with a wavelength of λ being 450nm is calculated to obtain:

[0092] OD 450 =[-0.075,-0.094,-0.094,-0.075]

[0093] Through the above method, fast and accurate detection of dry test strips is achieved. Utilizing the high-precision optical detection capability of the lens-type optical detection pen 2, specific areas on the test strip can be accurately detected. At the same time, the design of the test strip adapter 1 ensures that the ambient light during the detection process is effectively shielded, thereby ensuring the accuracy of the test results. In addition, the method also allows the user to view the test results in real time through the control terminal 3, and to perform further data processing and analysis, making the entire detection process more intuitive and easy to operate. In addition, the simplicity and accessibility of the above method are particularly suitable for large-scale on-site and personal and family self-testing, especially for infants and young children; blood oxygen, hemoglobin, jaundice and other tests can also be performed directly on the skin. Therefore, the application scenarios include the field, the field, individuals, families, and laboratories.

[0094] Example 2

[0095] The difference from Example 1 is that Figure 5 The standard grayscale card shown is the test object. Hold the lens-type optical detection pen 2 connected to the smartphone via a Type-C cable, and vertically cover the different grayscale areas of the standard grayscale card with its detection probe assembly 23 in turn; turn on the detection pen, control it from the smartphone, select a 405nm single-wavelength LED light source, set the photocurrent to 150, the gain to 7, and measure the reflected light intensity of different grayscale areas through a micro-spectrometer. The reflected light density is calculated using the reflected light intensity, and is displayed and stored on the smartphone; a standard curve is drawn between it and the grayscale level, and the results are shown in the attached Figure 5 , y=0.8369x+0.0056, R 2 =0.9996 (r=0.9998).

[0096] Similarly, using a combination of single-wavelength LEDs as the light source, Figure 5 The standard grayscale card shown is the test object, and its reflection to 450nm, 490nm, 540nm, 570nm, 620nm, 670nm, and 365nm light sources is measured respectively. Set the corresponding photocurrent and gain, and the measured standard curve and correlation coefficient square value are shown in the attached Figure 5 . The above results all show a good linear relationship. This proves that the detection device and detection method of the present application are not only applicable to standard grayscale cards, but can also be widely used in a variety of other detection scenarios. For example, by adjusting the wavelength and intensity of the light source module 22, the lens-type optical detection pen 2 can perform qualitative and quantitative analysis on different types of test papers. In actual applications, users can choose appropriate light sources and parameter settings as needed to adapt to different detection requirements.

[0097] Other details are the same as those in Example 1 and will not be described in detail in this application.

[0098] Example 3

[0099] like Figure 6 As shown, unlike Example 1, this example conducts an experiment of detecting uric acid level based on Fossati enzyme reaction on the pen-type optical detection device of the present application. According to the preparation of a series of uric acid standard solutions, uric acid of each concentration is converted into allantoin and different amounts of hydrogen peroxide under the action of uricase; hydrogen peroxide forms a red complex with 4-aminoantipyrine and TOOS [N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline] under the catalysis of peroxidase, and the level of the complex is proportional to the level of uric acid in the sample.

[0100] Furthermore, the detection reaction is carried out on a dry test paper. Insert the dry test paper into the corresponding card slot of the test paper adapter 1, and place the detection probe of the lens-type optical detection pen 2 in the detection area 11 of the test paper adapter 1. The area of ​​the circular detection area 11 is any one between 2-36 square millimeters; the detection pen communicates with the smart tablet via Bluetooth and is controlled by the tablet; after adding the sample, turn on the detection pen through the tablet, select the 540nm single-wavelength LED light source, and the photoelectric sensor collects the optical signal. The reflected light density of this complex at 540nm is measured by the endpoint method; data processing is performed based on the data presented on the smart tablet and a standard curve of uric acid levels is drawn. According to Figure 6 It can be seen from the experimental data that the pen-type optical detection device of the present application can accurately measure the uric acid level. As the uric acid concentration increases, the reflected light density also increases accordingly, showing a good linear relationship.

[0101] Other details that are the same as those in Example 1 are not described in detail in this example.

[0102] Example 4

[0103] like Figure 7 As shown, in order to further reflect the detection performance of the pen-type optical detection device of the present application, the present application adopts a pen-type optical detection device to identify the color of the color block of the standard color card. Specifically, the detection probe of the lens-type optical detection pen 2 is placed in the color block area to be tested on the standard color card. The detection pen communicates with the computer through the USB interface. When the computer is turned on, the reflection of each color block to be tested to blue light, green light, and red light (corresponding to wavelengths of 450nm, 540nm, and 670nm) is measured respectively. Taking white, black, blue, green, yellow, and red color blocks as examples, after the light source is selected, the photoelectric sensor collects the optical signal. The reflection data of the color block to the three colors of light are shown in the attached. Figure 7 After conversion, the color of the color block can be inferred by comparing the differences in the reflectance of red, green and blue light.

[0104] pass Figure 7It can be seen from the data that the pen-type optical detection device of the present application can accurately identify the colors of different color blocks and has high sensitivity and accuracy.

[0105] Other details that are the same as those in Example 1 are not described in detail in this example.

[0106] Example 5

[0107] In the present application, commercially available time-resolved fluorescent microspheres are diluted 100 times, counted as 100, and then diluted to 0.390625 based on this, and a blank control is set, with a total of 10 concentrations, which are dotted on the film in the observation area of ​​the test card; the test card is inserted into the corresponding card slot of the test paper adapter 1, and the detection probe of the lens-type optical detection pen 2 is placed in the detection window of the test paper adapter 1. The excitation light source is 365nm, and the multi-spectral array sensor is used with a filter to collect a 620nm light signal. At the same time, a 365nm light signal without a filter channel is received, and the 365nm and 620nm light intensities are detected respectively. The light intensity ratio of the 620nm light signal to the 365nm light signal is used to draw a curve against the concentration of the fluorescent nano-microspheres.

[0108] Figure 8 The images of 10 concentration points detected by the above detection method and the light intensity ratio of 620nm light signal to 365nm light signal versus the concentration of fluorescent nanospheres are shown. This proves that the pen-type optical detection device of the present application can accurately measure the concentration of fluorescent nanospheres. As the concentration of fluorescent nanospheres decreases, the light intensity ratio of 620nm light signal to 365nm light signal gradually decreases, showing a good linear relationship.

[0109] Other details that are the same as those in Example 1 are not described in detail in this example.

[0110] Example 6

[0111] Different from Example 1, the pen-type optical detection device of the present application can also directly measure the maturity of plant fruits according to the color difference of different maturity of plant fruits. Specifically, turn on the switch of the lens-type optical detection pen 2 (i.e., the button in Example 1), aim its detection probe at the fruit skin, use its color recognition function to detect the color of the fruit, and the smart tablet stores and converts the fruit maturity.

[0112] Other details that are the same as those in Example 1 are not described in detail in this example.

[0113] The above are only preferred embodiments of the present application and are not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper, characterized in that: It comprises a test paper adapter (1), a lens-type optical detection pen (2), and a control terminal (3) communicatively connected to the lens-type optical detection pen (2); The test paper adapter (1) is used to fix the test paper, and comprises at least two detection areas (11). The detection areas (11) are used for the lens-type optical detection pen (2) to perform detection and shield ambient light. The lens-type optical detection pen (2) detects the paper to be tested, and the testing process is active positioning and point-to-point testing; The control terminal (3) is used for program control and for receiving detection data transmitted by the lens-type optical detection pen (2).

2. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper according to claim 1, characterized in that: The lens-type optical detection pen (2) comprises a photoelectric detection module (21), a light source module (22), and a probe assembly (23) connected to the photoelectric detection module (21); The light source module (22) is used to provide light for irradiating the test paper through the probe assembly (23), and the photoelectric detection module (21) is used to collect the light signal of the light transmitted from the test paper back to the probe assembly (23) and convert the light signal into an electrical signal. The light signal includes reflected light, fluorescence, and chemiluminescence signals.

3. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper according to claim 2, characterized in that: The probe assembly (23) comprises a probe light shield (231) and a hemispherical lens (232) arranged in the light shield (231); the light shield (231) is provided with an annular gasket (2311), and the annular gasket (2311) is used for clamping the hemispherical lens (232).

4. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper according to claim 2, characterized in that: The lens-type optical detection pen (2) further comprises a main control module (24), wherein the main control module (24) receives the electrical signal transmitted by the photoelectric detection module (21), and the main control module (24) is communicatively connected with the control terminal (3).

5. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper according to claim 4, characterized in that: The main control module (24) comprises a power management module (241), and the power management module (241) is used to drive the main control module (24), the photoelectric detection module (21) and the light source module (22) to operate.

6. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper according to claim 2, characterized in that: The photoelectric detection module (21) comprises a photoelectric sensor, a filter and an ADC converter, wherein the photoelectric sensor is used to collect the light signal passing through the filter and convert the light signal into an electrical signal through the ADC converter.

7. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper according to claim 6, characterized in that: The photoelectric sensor is a multi-spectral array sensor or a micro-spectrometer or a micro-camera.

8. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper according to claim 2, characterized in that: The light source module is an annular light source composed of at least one single-wavelength LED or at least one full-spectrum white light LED; The single-wavelength LED and the full-spectrum white light LED can be controlled separately, and the wavelength range of the single-wavelength LED is: 365nm-620nm.

9. A pen-type optical detection device for qualitative and quantitative analysis of dry test paper according to claim 4, characterized in that: The main control module (24) comprises an integrated control circuit board (242) and a circuit board fixing member (243); The lens-type optical detection pen (2) further comprises a housing (25) and a cover plate (26) covering the top of the housing (25); the integrated control circuit board (242) and the circuit board fixing member (243) are both arranged in the housing (25).

10. A detection method for qualitative and quantitative analysis of dry test paper, comprising a detection device for qualitative and quantitative analysis of dry test paper as claimed in any one of claims 1 to 9, characterized in that: The photoelectric collection module (21) collects the light intensity of at least one wavelength and outputs a corresponding electrical signal to the main control module (24); the main control module (24) preprocesses the electrical signal and calculates the optical density value of the preprocessed data signal; the main control module (24) transmits the preprocessed electrical signal and the optical density value to the control terminal (3); The pretreatment method is: Where I(n) represents the value of the electrical signal at time point n, is the preprocessed electrical signal, N represents the window length of the moving average of the above processing method, K is a variable used for indexing, and represents the offset of each data point used in the accumulation process; The optical density value calculation method is: Among them, OD λ is the optical density value at wavelength λ, where is the light intensity value with wavelength λ after the above preprocessing, I incident，λ The light source is emitted by the light source module (22) with a wavelength of λ.

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