Photoelectric integrated biosensor and biomolecule detection equipment

Abstract

The invention relates to the technical field of biosensors, in particular to a photoelectric integrated biosensor and biomolecule detection device.The photoelectric integrated biosensor comprises a substrate, a detection module is arranged on the substrate, and a light source module used for emitting light with different illumination intensities is arranged below the substrate; the detection module comprises a solution pre-separation module located on the base body, the solution pre-separation module comprises a liquid storage plate used for centrifugally separating a sample solution, and the periphery of the liquid storage plate is communicated with a plurality of liquid conveying pipes used for conveying centrifuged solutions with different masses; the infusion tubes are respectively communicated with a measuring cavity, and an electrode capturing module for measuring light current intensity corresponding to solutions with different masses is arranged in the measuring cavity; the method is used for improving the detection stability in the detection process and reducing measurement errors.

Description

Technical Field

[0001] The present invention relates to the technical field of biosensors, and in particular to a photoelectric integrated biosensor and a biomolecule detection device. Background Art

[0002] Optoelectronic integrated biosensor is a sensor technology that combines optical and electronic technologies. It has been widely used in many fields such as biomolecular analysis, environmental testing, food safety, new drug research, medical care, etc.

[0003] Existing optoelectronic integrated biosensors usually consist of a light excitation source, a three-electrode system (detection system) and a signal reading device. The three-electrode system usually uses a photoactive material as a substrate, and attaches biological components with specific recognition capabilities to the electrode material. Under light conditions, when biological molecules react specifically with the target, the photocurrent signal will change accordingly. Since the concentration of the target is closely related to the change in the photocurrent signal, the target can be accurately identified and quantitatively analyzed by the change in the output photocurrent signal.

[0004] However, in actual application, due to the complex and changeable working environment of the sensor, other non-specific substances may bind to the electrode. These non-specific substances may come from impurities in the sample solution, contamination on the electrode surface, or residues in the sensor preparation process, which may interfere with the accuracy and reliability of the sensor. Therefore, the present invention provides a photoelectric integrated biosensor and biomolecule detection equipment with anti-interference ability to improve the stability during detection and reduce measurement errors. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a photoelectric integrated biosensor and a biomolecule detection device, which are used to improve the detection stability during the detection process and reduce the measurement error.

[0006] In order to achieve the above-mentioned object, the technical scheme of the present invention is as follows: a photoelectric integrated biosensor comprises a substrate, a detection module is arranged on the substrate, and a light source module for emitting different light intensities is arranged below the substrate; the detection module comprises a solution pre-separation module located on the substrate, the solution pre-separation module comprises a liquid storage plate for centrifugally separating a sample solution, and the liquid storage plate is connected with a plurality of infusion tubes for conveying solutions of different masses after centrifugation around the liquid storage plate;

[0007] The infusion tubes are respectively connected to measuring cavities, and the measuring cavities are provided with electrode capture modules for measuring the corresponding photocurrent intensities of solutions of different masses.

[0008] Furthermore, the solution pre-separation module includes a centrifugal chamber located at the center of the liquid storage plate, the centrifugal chamber is used to store the sample solution, and connecting grooves are evenly arranged around the centrifugal chamber in the height direction, and an opening and closing mechanism for blocking the connecting grooves is provided in the connecting grooves;

[0009] The communicating grooves are respectively connected with infusion tubes, one end of the infusion tubes away from the communicating grooves is connected with the measuring cavity, and the measuring cavity is connected with a negative pressure tube for generating negative pressure suction.

[0010] Furthermore, a partition is slidably fitted on the base, and a transfer hole connected to the infusion tube is opened on the partition; a normal pressure cavity connected to the outside atmosphere is provided below the partition, and an extension block fixedly connected to the base is provided above the partition, and a negative pressure cavity connected to the measuring cavity is opened in the extension block, and a negative pressure tube is connected between the negative pressure cavity and the measuring cavity, and the negative pressure tube is located above the infusion tube;

[0011] A push rod is fixedly connected to the top of the partition, and a pressure cover is provided on the top of the negative pressure chamber, which is clamped with the extension block;

[0012] When the pressure in the negative pressure chamber is in normal state, the transfer hole is separated from the infusion tube; when the pressure in the negative pressure chamber is in negative pressure state, the transfer hole is connected with the infusion tube; and when the partition moves to the top, the push rod and the pressure cover are against each other.

[0013] Furthermore, the height of the measuring cavity is lower than that of the centrifugal cavity, and a concave chamber is connected to the measuring cavity, which is located below the end of the infusion tube away from the centrifugal cavity; the electrode capture module is located above the concave chamber.

[0014] Furthermore, it also includes a processing module, which is used to enter and store the height mark of the infusion tube connecting the centrifugal cavity corresponding to the electrode capture module, and compare the photocurrent intensity with the comparison photocurrent intensity corresponding to the current target object. If the photocurrent intensity is similar to the comparison photocurrent intensity, then the target layer mark is added based on the height mark corresponding to the electrode capture module; if the photocurrent intensity is not similar to the comparison photocurrent intensity, then the impurity layer mark is added based on the height mark corresponding to the electrode capture module.

[0015] Furthermore, the light source module includes a plurality of irradiation modules corresponding to the measuring cavity, the irradiation modules are located below the substrate, and the irradiation modules are used to change the irradiation intensity of different light wavelengths;

[0016] The processing module is also used to enter and store the position mark corresponding to the measuring cavity, which corresponds to the height mark and the position of the irradiation module, and then obtain the position mark corresponding to the corresponding measuring cavity based on the current target layer mark, and adjust the irradiation intensity of the corresponding irradiation module based on the position mark.

[0017] Furthermore, the opening and closing mechanism includes a screw rod and a nut seat, and the nut seat and the screw rod are slidably matched; the screw rod and the nut seat are located on both sides of the connecting groove, and the nut seat is fixedly connected with an elastic baffle plate, and the end of the baffle plate away from the nut seat is conical, and a magnet layer and an electromagnet are provided between adjacent baffle plates, the magnet layer is fixedly connected to the baffle plate on one side, and the electromagnet is fixedly connected to the baffle plate on the other side.

[0018] Furthermore, a transmittance tester for measuring the transmittance of the sample solution in the centrifugal chamber at different heights is provided around the liquid storage plate;

[0019] The processing module is also used to obtain a mass solution with consistent transmittance based on the target mark and the impurity layer mark, obtain the layer height corresponding to the mass solution with consistent transmittance, and add the target mark based on the layer height.

[0020] Furthermore, the processing module is also used to compare the layer height with the corresponding standard value. If the layer height is greater than the standard value, a standby instruction is sent to the opening and closing mechanism; if the layer height is less than the standard value, the difference ratio between the layer height and the standard value is calculated, and a start instruction is sent to the opening and closing mechanism based on the difference ratio.

[0021] Furthermore, a biomolecule detection device includes a negative pressure pump for generating negative pressure suction, the output end of the negative pressure pump is connected to the negative pressure tube, and the above-mentioned photoelectric integrated biosensor.

[0022] The above scheme has the following beneficial effects:

[0023] 1. In this scheme, a solution pre-separation module is used to centrifuge the sample solution to separate the sample solution from other impurity solutions, thereby reducing the impact of non-specific results of other impurities on subsequent measurement of photocurrent intensity and reducing measurement errors.

[0024] 2. This scheme verifies specific binding by obtaining a solution with a high content of the target component, and performs auxiliary verification by generating light intensities of different wavelengths, so as to further use the photocurrent intensity to accurately identify and quantitatively analyze the target, thereby reducing measurement errors.

[0025] 3. This scheme utilizes the height and content characteristics of the sample solution during separation to separate the target from the impurities, thereby providing error generation in the subsequent measurement process.

[0026] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1An axonometric diagram of an embodiment of the optoelectronic integrated biosensor of the present invention;

[0028] Figure 2 A top view of an embodiment of a photoelectric integrated biosensor of the present invention;

[0029] Figure 3 for Figure 2 Schematic diagram of the cross section in the AA direction;

[0030] Figure 4 for Figure 3 A magnified schematic diagram of the local A in the middle;

[0031] Figure 5 for Figure 3 Enlarged schematic diagram of part B in the middle.

[0032] The figure marks in the drawings of the specification include: 1. base; 11. measuring cavity; 12. extension block; 13. pressure cover; 14. concave chamber; 2. liquid storage plate; 21. motor; 22. baffle plate; 23. connecting groove; 24. screw rod; 25. nut seat; 3. infusion tube; 31. partition; 32. transfer hole; 33. negative pressure chamber; 34. normal pressure chamber; 4. negative pressure tube; 5. electrode capture module. DETAILED DESCRIPTION

[0033] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0035] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0036] The following is further described in detail through specific implementation methods:

[0037] Embodiment 1:

[0038] As attached Figures 1 to 5 As shown: a photoelectric integrated biosensor includes a substrate 1. In this embodiment, the substrate 1 is made of a transparent semiconductor material, such as silicon. A detection module is provided on the substrate 1, and a light source module (not shown in the figure) for emitting different light intensities is provided below the substrate 1. The light source module includes a plurality of irradiation modules (not shown in the figure) corresponding to the measuring cavity 11. The irradiation module is located below the substrate 1, and the irradiation module is used to change the irradiation intensity of different light wavelengths.

[0039] The detection module includes a solution pre-separation module located on the substrate, and the solution pre-separation module includes a liquid storage plate 2 for centrifugation and separation of sample solutions, a motor 21 is coaxially fixedly connected to the bottom of the liquid storage plate 2, and the motor 21 is fixedly connected to the substrate screws, and a plurality of infusion tubes 3 for conveying centrifuged solutions of different masses are connected around the liquid storage plate 2. The infusion tubes 3 are respectively connected to the measuring chambers 11, and the measuring chambers 11 are opened inside the substrate, and the measuring chambers 11 are provided with electrode capture modules 5 for measuring the photocurrent intensity corresponding to solutions of different masses.

[0040] Among them, the solution pre-separation module includes a centrifugal chamber located at the center of the liquid storage plate 2, the centrifugal chamber is used to store the sample solution, and connecting grooves 23 are evenly arranged around the centrifugal chamber along the height direction. The connecting grooves 23 at different height directions are staggered with each other. An opening and closing mechanism for blocking the connecting grooves 23 is provided in the connecting grooves 23. In this embodiment, the opening and closing mechanism is an electromagnetic valve; the connecting grooves 23 are respectively connected to infusion tubes 3, and the ends of the infusion tubes 3 away from the connecting grooves 23 are connected to the measuring chamber 11, and the measuring chamber 11 is connected to a negative pressure tube 4 for generating negative pressure suction.

[0041] It also includes a processing module (not shown in the figure), which is used to enter and store the height mark of the infusion tube 3 connecting the centrifugal cavity corresponding to the electrode capture module 5, and compare the photocurrent intensity with the comparison photocurrent intensity corresponding to the current target object. If the photocurrent intensity is similar to the comparison photocurrent intensity, a target layer mark is added based on the height mark corresponding to the electrode capture module 5; if the photocurrent intensity is not similar to the comparison photocurrent intensity, an impurity layer mark is added based on the height mark corresponding to the electrode capture module 5.

[0042] For example, during the target detection process, the corresponding height is obtained when the centrifugal chamber is connected to the infusion tube 3, so that the corresponding target distribution area can be obtained in time and the fixed-point height collection and comparison can be carried out subsequently, so as to facilitate the subsequent experimental comparison to verify the concentration and other characteristics of the target.

[0043] The processing module is also used to enter and store the position mark corresponding to the measuring cavity 11, which corresponds to the height mark and the position of the irradiation module, and then obtain the position mark corresponding to the corresponding measuring cavity 11 based on the current target layer mark, and adjust the irradiation intensity of the corresponding irradiation module based on the position mark.

[0044] For example, in the subsequent detection process, the mass solution in the measuring cavities 11 on both sides is irradiated by changing the irradiation intensity of different wavelengths to obtain the corresponding photocurrent intensity. The wavelength of light in the measuring cavity 11 on one side is modulated by the target object parameters, while the wavelength of light in the measuring cavity 11 on the other side is not modulated by the target object parameters, so as to obtain a measurement value with reduced error and improve the stability and measurement accuracy of the sensor.

[0045] In this embodiment, a biomolecule detection device is also provided, including a negative pressure pump for generating negative pressure suction, the output end of the negative pressure pump is connected to the negative pressure tube 4, and the above-mentioned photoelectric integrated biosensor.

[0046] The specific implementation process is as follows: first, the sample solution is introduced into the centrifugal chamber in the liquid storage plate 2, the opening and closing mechanism closes the connecting groove 23, and the liquid storage plate 2 is driven to rotate by the motor 21 to produce a centrifugal separation effect, so that the target object is separated from the impurities in the sample solution to form a mass solution with different mass layers; so as to facilitate the separation of the sample solution and other impurity solutions, so as to reduce the influence of the non-specific results of other impurities on the subsequent measurement of the photocurrent intensity, so as to reduce the measurement error.

[0047] After the centrifugal separation of the sample solution is completed, the connecting groove 23 is connected to the corresponding infusion tube 3, and the negative pressure pump is started to suck the inside of the measuring cavity 11 to generate negative pressure suction, and then the opening and closing mechanism is opened to suck the mass solution of the corresponding height through the negative pressure suction, and the mass solution containing the target object at different heights is determined based on the photocurrent intensity. In the subsequent verification process, the corresponding opening and closing mechanism and the negative pressure pump are started based on the height of the mass solution to perform special suction, and the illumination intensity of different wavelengths is used for experimental comparison to reduce or eliminate measurement errors, thereby improving the stability and measurement accuracy of the sensor.

[0048] Embodiment 2:

[0049] The difference from Example 1 lies in that a partition 31 is slidably fitted on the base, and a transfer hole 32 connected to the infusion tube 3 is opened on the partition 31; a normal pressure chamber 34 connected to the outside atmosphere is provided below the partition 31 (the normal pressure chamber 34 is opened in the base), and an extension block 12 fixedly connected to the base is provided above the partition 31, and a negative pressure chamber 33 connected to the measuring chamber 11 is opened in the extension block 12, and a negative pressure tube 4 is connected between the negative pressure chamber 33 and the measuring chamber 11, and the negative pressure tube 4 is located above the infusion tube 3; a push rod is fixedly connected to the top of the partition 31, and a pressure cover 13 is provided on the top of the negative pressure chamber 33, and the pressure cover 13 is snap-connected with the extension block 12; when the pressure in the negative pressure chamber 33 is in normal state, the transfer hole 32 is separated from the infusion tube 3; when the pressure in the negative pressure chamber 33 is in a negative pressure state, the transfer hole 32 is connected to the infusion tube 3; and when the partition 31 moves to the top, the push rod and the pressure cover 13 are against each other.

[0050] The specific implementation process is as follows: according to the changes in the operation process, when the centrifugation of the sample solution in the centrifugal chamber is completed, the opening and closing mechanism is opened to connect with the infusion tube 3, and a negative pressure is formed in the measuring chamber 11 through the negative pressure tube 4. At this time, the transfer hole 32 is connected with the infusion tube 3 to automatically suck the different mass solutions after centrifugation into the measuring chamber 11 for detection; when the sample solution in the infusion tube 3 enters the measuring chamber 11, the negative pressure tube 4 continuously sucks the air inside the negative pressure chamber 33, and the pressure balance between the negative pressure chamber 33 and the normal chamber is broken. The pressure difference overcomes the self-gravity of the partition 31 and moves upward, and the transfer hole 32 is separated from the infusion tube 3, so as to automatically realize the sealing treatment of the infusion tube 3, reduce the subsequent sample solutions of different mass solutions entering the infusion tube 3 and mixing, so as to reduce the measurement error in the detection process.

[0051] Embodiment 3:

[0052] The difference from Example 2 is that the position height of the measuring cavity 11 is lower than the position height of the centrifugal cavity, and the measuring cavity 11 is connected to a concave chamber 14, which is located below the end of the infusion tube 3 away from the centrifugal cavity; the electrode capture module 5 is located above the concave chamber 14.

[0053] The specific implementation process is as follows: during the process of liquid transportation by the infusion tube 3, when the sample solution inside the infusion tube 3 is discharged into the measuring cavity 11 by gravity, due to the impact force generated by the sample solution itself, the bottom circular groove is used to guide the liquid discharged from the infusion tube 3 to form an upward reflux to contact the electrode capture module 5, so as to facilitate the contact reaction between the sample solution and the electrode capture module 5.

[0054] Embodiment 4:

[0055] The difference from Example 3 is that the opening and closing mechanism includes a screw rod 24 and a nut seat 25, and the nut seat 25 is slidably matched with the screw rod 24; the screw rod 24 and the nut seat 25 are located on both sides of the connecting groove 23, and the nut seat 25 is fixedly connected with an elastic baffle plate 22, and the end of the baffle plate 22 away from the nut seat 25 is conical, and a magnet layer and an electromagnet are provided between adjacent baffle plates 22, the magnet layer is fixedly connected to the baffle plate 22 on one side, and the electromagnet is fixedly connected to the baffle plate 22 on the other side.

[0056] Under normal conditions, the rotation of the lead screw 24 is utilized to push the baffle plate 22 away from or toward the connecting groove 23. At this time, the adjacent baffle plates 22 are held fixed by the adsorption of the magnetic layer, so as to contact the sample solution inside the centrifugal chamber through the conical block, so as to separate the sample solution on both sides of the baffle plate 22; by starting the electromagnet and pushing the adjacent baffle plates 22 to separate, a slit is formed between the baffle plates 22, so that the sample solution at the current height can enter the infusion tube 3, so as to reduce the mass solution containing impurities at different height directions from entering the infusion tube 3, so as to reduce the interference of impurities, so as to improve the stability and measurement accuracy of the sensor.

[0057] The liquid storage plate 2 is surrounded by transmittance testers for measuring the corresponding transmittance of the sample solution in the centrifugal chamber at different heights; the processing module is also used to obtain a quality solution with consistent transmittance based on the target mark and the impurity layer mark, and obtain the layer height corresponding to the mass solution with consistent transmittance, and add the target mark based on the layer height.

[0058] For example, after centrifugation, the mass solution after stratification is determined by the change in transmittance to determine the heights corresponding to solutions of different masses, so as to control the corresponding infusion tubes 3 for corresponding communication, so as to accurately obtain the stratification corresponding to the target layer.

[0059] The processing module is also used to compare the layer height with the corresponding standard value. If the layer height is greater than the standard value, a standby instruction is sent to the opening and closing mechanism; if the layer height is less than the standard value, the difference ratio between the layer height and the standard value is calculated, and a start instruction is sent to the opening and closing mechanism based on the difference ratio.

[0060] For example, based on the movement of the above-mentioned opening and closing mechanism, the layer heights are compared through the processing module, and the extension length of the shielding plate 22 is controlled in the narrower sample solution layer, so that the shielding plate 22 can penetrate deep into the sample solution layer for sampling, so as to obtain the narrow sample solution.

[0061] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the present invention.

Claims

1. A photoelectric integrated biosensor, comprising a substrate (1), a detection module being arranged on the substrate (1), and a light source module for emitting light of different intensities being arranged below the substrate (1); characterized in that: The detection module comprises a solution pre-separation module located on a substrate, the solution pre-separation module comprises a liquid storage plate (2) for centrifugally separating a sample solution, and the liquid storage plate (2) is connected around a plurality of infusion tubes (3) for conveying solutions of different masses after centrifugation; The infusion tubes (3) are respectively connected to the measuring cavities (11), and the measuring cavities (11) are provided with electrode capture modules (5) for measuring the corresponding photocurrent intensities of solutions of different masses.

2. The optoelectronic integrated biosensor according to claim 1, characterized in that: The solution pre-separation module comprises a centrifugal chamber located at the center of the liquid storage plate (2), the centrifugal chamber is used to store the sample solution, and connecting grooves (23) are evenly arranged around the centrifugal chamber in the height direction, and an opening and closing mechanism for blocking the connecting grooves (23) is provided in the connecting grooves (23); The communication grooves (23) are respectively connected to infusion tubes (3), one end of the infusion tube (3) away from the communication grooves (23) is connected to the measuring cavity (11), and the measuring cavity (11) is connected to a negative pressure tube (4) for generating negative pressure suction. The air inside the measuring chamber (11) is sucked through the negative pressure tube (4), and negative pressure suction is formed in the connecting groove (23) and the measuring chamber (11). The inside of the centrifugal chamber is connected to the infusion tube (3) by opening the opening and closing mechanism, and the negative pressure suction is used to suck the centrifugal chamber to extract the mass solutions after different layers after centrifugation, so as to facilitate subsequent monitoring.

3. The optoelectronic integrated biosensor according to claim 2, characterized in that: A partition (31) is slidably fitted on the base, and a transfer hole (32) is formed on the partition (31) and is connected to the infusion tube (3); a normal pressure cavity (34) connected to the outside atmosphere is provided below the partition (31); an extension block (12) fixedly connected to the base is provided above the partition (31); a negative pressure cavity (33) connected to the measuring cavity (11) is formed in the extension block (12); a negative pressure tube (4) is connected between the negative pressure cavity (33) and the measuring cavity (11), and the negative pressure tube (4) is located above the infusion tube (3); A push rod is fixedly connected to the top of the partition (31), a pressure cover (13) is provided on the top of the negative pressure chamber (33), and the pressure cover (13) is clamped with the extension block (12); When the pressure in the negative pressure chamber (33) is in a normal state, the transfer hole (32) is separated from the infusion tube (3); when the pressure in the negative pressure chamber (33) is in a negative pressure state, the transfer hole (32) is connected to the infusion tube (3); and when the partition (31) moves to the top, the push rod and the pressure cover (13) are in contact with each other.

4. The optoelectronic integrated biosensor according to claim 3, characterized in that: The height of the measuring cavity (11) is lower than that of the centrifugal cavity; the measuring cavity (11) is connected to a concave chamber (14); the concave chamber (14) is located below an end of the infusion tube (3) away from the centrifugal cavity; and the electrode capture module (5) is located above the concave chamber (14).

5. The optoelectronic integrated biosensor according to claim 4, characterized in that: The method further comprises a processing module, wherein the processing module is used to input and store the height mark of the infusion tube (3) connected to the centrifugal cavity corresponding to the electrode capture module (5), and to compare the photocurrent intensity with the comparison photocurrent intensity corresponding to the current target object for similarity; if the photocurrent intensity is similar to the comparison photocurrent intensity, a target layer mark is added based on the height mark corresponding to the electrode capture module (5); if the photocurrent intensity is not similar to the comparison photocurrent intensity, an impurity layer mark is added based on the height mark corresponding to the electrode capture module (5).

6. The optoelectronic integrated biosensor according to claim 5, characterized in that: The light source module comprises a plurality of irradiation modules corresponding to the measurement cavity (11), the irradiation modules are located below the substrate (1), and the irradiation modules are used to change the irradiation intensity of different light wavelengths; The processing module is also used to input and store the position mark corresponding to the measuring cavity (11), the position mark corresponding to the height mark and the position of the irradiation module, and then obtain the position mark corresponding to the corresponding measuring cavity (11) based on the current target layer mark, and adjust the irradiation intensity of the corresponding irradiation module based on the position mark.

7. The optoelectronic integrated biosensor according to claim 6, characterized in that: The opening and closing mechanism comprises a screw rod (24) and a nut seat (25), and the nut seat (25) and the screw rod (24) are slidably matched; the screw rod (24) and the nut seat (25) are located on both sides of the connecting groove (23); an elastic shielding plate (22) is fixedly connected to the nut seat (25); one end of the shielding plate (22) away from the nut seat (25) is conical; a magnet layer and an electromagnet are provided between adjacent shielding plates (22); the magnet layer is fixedly connected to the shielding plate (22) on one side, and the electromagnet is fixedly connected to the shielding plate (22) on the other side.

8. The optoelectronic integrated biosensor according to claim 7, characterized in that: A light transmittance tester for measuring the light transmittance of the sample solution in the centrifugal chamber at different heights is arranged around the liquid storage plate (2); The processing module is also used to obtain a mass solution with consistent transmittance based on the target mark and the impurity layer mark, obtain the layer height corresponding to the mass solution with consistent transmittance, and add the target mark based on the layer height.

9. The optoelectronic integrated biosensor according to claim 8, characterized in that: The processing module is also used to compare the layer height with the corresponding standard value. If the layer height is greater than the standard value, a standby instruction is sent to the opening and closing mechanism; if the layer height is less than the standard value, the difference ratio between the layer height and the standard value is calculated, and a start instruction is sent to the opening and closing mechanism based on the difference ratio.

10. A biomolecule detection device, characterized in that: It comprises a negative pressure pump for generating negative pressure suction, the output end of the negative pressure pump is connected to a negative pressure tube (4), and a photoelectric integrated biosensor as claimed in any one of claims 1 to 9.

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