Continuous sample injection propofol blood concentration analyzer detection system

Abstract

The invention discloses a detection system of a continuous sample injection propofol blood concentration analyzer. The detection system comprises an automatic continuous blood sampling device, a thermal desorption sample injector, an ion migration tube detector and a signal receiving and processing device, the ion migration tube detector is provided with a gas outlet, a sample carrier gas inlet, a drift gas inlet and a chemical dopant inlet, the gas outlet is connected with a sucking pump, and the gas path system is in a pump sucking sampling mode; the automatic continuous blood sampling device is used for collecting blood stored in the in-vitro blood storage chamber, one end of the thermal desorption sample injector is connected with the automatic continuous blood sampling device, and the other end of the thermal desorption sample injector is connected with a sample carrier gas inlet of the ion migration tube detector through a gas path connecting tube; and the signal output end of the ion migration tube detector is connected with the signal receiving and processing device. Compared with manual sample introduction, the device can reduce the workload of a user and efficiently and intelligently realize blood sample analysis.

Description

Technical Field

[0001] The invention belongs to the field of drug concentration analysis and detection systems in blood, and in particular relates to a continuous sampling propofol blood drug concentration analyzer detection system. Background Art

[0002] Real-time dynamic monitoring of blood drug concentration is a key and difficult problem in studying the mechanism of drug action on the patient's body and the method of precise drug administration. There is no effective real-time and online analytical method at home and abroad. Chromatography and mass spectrometry are currently used to monitor blood drug concentration at home and abroad. Since these methods are time-consuming, labor-intensive, and have complex pre-treatment, there are only a few reports at home and abroad, and they are rarely used in clinical practice. Ion Mobility Spectrometry (IMS) technology is a rapid separation and detection technology that appeared in the 1970s. Compared with traditional mass spectrometry and chromatography instruments, it has the characteristics of simple structure, high sensitivity, fast analysis speed, and reliable results. The IMS we are studying has been widely used in many fields. The ion migration tube is the core component of the ion migration spectrometer. As an ion separation and detector, it usually consists of five parts: ionization source, reaction zone, ion gate, migration zone, and Faraday disk. The ion source allows sample molecules, N 2 , O 2Ionization with water vapor, the generated ions can easily react with molecules to produce a variety of product ions. Driven by the electric field, the ions enter the migration zone through the periodically opened ion gate and continuously collide with the countercurrent neutral floating gas molecules. Since these ions have different migration rates in the electric field, different ions are separated and arrive at the detector one after another. It is worth noting that due to the differences in chemical properties such as ionization energy, proton affinity, and electronegativity of different compounds, the efficiency and selectivity of producing positive and negative polarity ions when ionized in the atmospheric pressure ionization source are different. The risk of radioactive contamination caused by traditional 63Ni ionization sources in clinical applications is not conducive to the safety of on-site application in the operating room and large-scale clinical promotion. Extracorporeal circulation is a life support technology that uses a series of special artificial devices to drain the return heart venous blood to the body, exchange gases through artificial methods, adjust the temperature and filter, and then return it to the body's arterial system. In the process of extracorporeal circulation, since the artificial device replaces the human body's function, it is also called cardiopulmonary bypass, and the extracorporeal circulation machine is also called an artificial heart-lung machine. The purpose of extracorporeal circulation is to maintain the blood supply of tissues and organs throughout the body during open heart surgery. With the development of clinical medicine, the application scope of extracorporeal circulation has been continuously expanded. It has not only been applied in major vascular surgeries such as heart, liver, kidney, and lung, but has also achieved remarkable results in the treatment of tumors and life support for patients with cardiopulmonary failure, becoming an important technology in clinical medicine. Propofol, an intravenous anesthetic, has no simple and fast intraoperative real-time monitoring technology at home and abroad. At present, the team of the inventor in China has developed a manual interval blood sampling and sampling device based on ion mobility spectrometry technology, and the authorized patent "ZL201510736154.1, a propofol online detector in blood and its application", and the research results have been transformed. In the future, the research and development of automatic blood sampling and detection systems will inevitably lead to a revolution in the field of blood drug concentration analysis, promote innovative technologies and innovative theories in related fields, and are the only key technology to achieve accurate and safe medication in clinical practice. Summary of the invention

[0003] The purpose of the present invention is to provide a continuous sampling propofol blood concentration analyzer detection system, which is based on ion mobility spectrometry technology to analyze and detect the drug concentration in the blood after the clinician administers anesthetics during extracorporeal circulation surgery. The detection system of the present invention can be widely used for in-depth analysis of clinical anesthesia administration and guide clinicians to use drugs accurately.

[0004] In order to achieve the above object, the technical solution of the present invention is as follows:

[0005] On one hand, the present invention provides a continuous sampling propofol blood concentration analyzer detection system, comprising an automatic continuous blood sampling device, a thermal desorption injector, an ion transfer tube detector, and a signal receiving and processing device;

[0006] The ion transfer tube detector is provided with an air outlet connected to an air pump, a sample carrier gas inlet, a drift gas inlet and a chemical dopant inlet, and the gas circuit system is in a pump air pumping and sampling mode;

[0007] The automatic continuous blood sampling device collects blood stored in an extracorporeal blood storage chamber, one end of the thermal desorption injector is connected to the automatic continuous blood sampling device, and the other end of the thermal desorption injector is connected to a sample carrier gas inlet of an ion transfer tube detector via a gas connection tube;

[0008] The signal output end of the ion transfer tube detector is connected to a signal receiving and processing device.

[0009] In the above technical scheme, further, the automatic continuous blood sampling device includes a peristaltic pump, a swing arm machine and a waste liquid bottle; the extracorporeal blood storage chamber supplies blood to the thermal desorption injector through the peristaltic pump, and an swing arm machine is externally connected to the blood transmission tube between the peristaltic pump and the thermal desorption injector, and the swing arm machine is connected to the waste liquid bottle.

[0010] In the above technical solution, further, the ionization source of the ion transfer tube detector is a VUV light ionization source.

[0011] In the above technical solution, further, the gas outlet, chemical dopant inlet and sample carrier gas inlet are located near the VUV lamp ionization source, and are respectively the gas outlet, chemical dopant inlet and sample carrier gas inlet according to the distance from the lamp from near to far.

[0012] On the other hand, the present invention provides a continuous sampling propofol blood concentration detection method, which utilizes the above-mentioned analyzer detection system, wherein the blood in the extracorporeal blood storage chamber is collected by an automatic continuous blood sampling device, the blood sample is vaporized in the thermal desorption chamber of the thermal desorption injector, and the gas is pumped by a vacuum pump into an ion migration tube detector for detection and analysis.

[0013] In the above technical solution, further, the blood entering the thermal desorption injector is controlled by a swing arm machine, and the excess blood is discharged into a waste liquid bottle as waste liquid.

[0014] The beneficial effects of the present invention are:

[0015] 1. The present invention can realize automatic blood collection without manual operation through the automatic continuous blood sampling device, help to realize automatic analysis of the blood concentration of the anesthetic propofol, replace the manual interval blood sampling method to reduce the user's workload, and realize blood sample analysis efficiently and intelligently.

[0016] 2. The present invention controls the blood collection volume through a peristaltic pump and controls blood collection or discharges excess waste blood through a swing arm machine. The peristaltic pump and the swing arm machine cooperate to achieve automatic blood collection and interval detection.

[0017] 3. The gas path system of the ion mobility spectrometer in the present invention adopts a pump suction sampling mode, and the sample gas is pumped into the detector, replacing the thermal desorption purge sampling mode, with high sensitivity and short analysis time.

[0018] 4. The detection system in the present invention is based on ion mobility spectrometry technology, combined with vacuum ultraviolet photoionization technology, to avoid radioactive contamination of the 63Ni source, which is convenient for safety of on-site application in the operating room and large-scale clinical promotion.

[0019] 5. The present invention improves ionization efficiency and selectivity by adding a dopant to an ionization source. The photons emitted by the photoionization source ionize high-concentration dopant (such as acetone and butanone) molecules to generate a large number of photoelectrons and dopant positive ions. In the atmospheric environment, these photoelectrons and dopant positive ions can generate reagent ions with different positive and negative polarities through a series of ion-molecule reactions. Subsequently, these reagent ions are effectively ionized with the propofol molecules to be detected through a chemical ionization reaction to generate characteristic ions of different polarities, and finally separation and detection are achieved through an ion transfer tube according to different mobilities. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a structural schematic diagram of a continuous sampling propofol blood concentration analyzer detection system;

[0021] In the figure: 1. vacuum pump, 2. air outlet, 3. chemical dopant inlet, 4. thermal desorption injector, 5. in vitro blood storage chamber, 6. peristaltic pump, 7. swing arm machine, 8. waste liquid bottle, 9. floating gas inlet, 10. ion migration tube detector, 11. sample carrier gas inlet. DETAILED DESCRIPTION

[0022] The following examples may enable those skilled in the art to more fully understand the present invention, but are not intended to limit the present invention in any way.

[0023] A continuous sampling propofol blood concentration analyzer detection system, such as Figure 1 As shown, it includes an automatic continuous blood sampling device, a thermal desorption sample injector 4, an ion transfer tube detector 10, and a signal receiving and processing device;

[0024] The ionization source of the ion transfer tube detector 10 is a VUV light ionization source. The outer wall of the ion transfer tube detector 10 is provided with four gas path holes, namely, a gas outlet 2, a chemical dopant inlet 3, a sample carrier gas inlet 11 and a drift gas inlet 9. The area close to the VUV light ionization source has three gas path holes in sequence. The three gas path holes are the gas outlet 2, the chemical dopant inlet 3 and the sample carrier gas inlet 11 in order from near to far from the lamp. The gas outlet 2 is connected to a vacuum pump 1. The gas path system is a pump suction sampling mode, and the gas flow is controlled by a mass flow controller.

[0025] The automatic continuous blood sampling device collects blood stored in an extracorporeal blood storage chamber 5. The automatic continuous blood sampling device includes a peristaltic pump 6, an arm swinging machine 7 and a waste liquid bottle 8. One end of a thermal desorption sampler 4 is connected to the peristaltic pump 6. The extracorporeal blood storage chamber 5 supplies blood to the thermal desorption sampler 4 through the peristaltic pump 6. The other end of the thermal desorption sampler 4 is connected to a sample carrier gas inlet 11 of an ion migration tube detector 10 through an air path connecting tube. An arm swinging machine 7 is externally connected to the blood transmission tube between the peristaltic pump 6 and the thermal desorption sampler. The arm swinging machine 7 controls the blood in the blood transmission tube, and the excess blood is discharged into the waste liquid bottle by the arm swinging machine.

[0026] The signal output end of the ion transfer tube detector 10 is connected to a signal receiving and processing device, which includes a signal amplifier, an A / D converter, and a PC processor.

[0027] A method for detecting the blood concentration of propofol by continuous sampling, wherein an extracorporeal blood storage chamber 5 stores blood in the patient's body during surgery, a blood transmission tube of a peristaltic pump 6 is connected to the extracorporeal blood storage chamber 5 through a joint, and the peristaltic pump 6 controls the blood collection volume per unit time by setting parameters, and the blood in the blood transmission tube of the peristaltic pump 6 is directly dripped into a thermal desorption injector 4, and the blood sample is gasified in the thermal desorption chamber, and the gas is pumped into the ion transfer tube detector 10 by a suction pump 1 connected to the gas outlet 2 of the ion transfer tube detector 10 for the next step of detection and analysis, and a PC displays the final detection result, and the peristaltic pump 6 is still working and rotating during the detection and analysis process, and at this time, the excess blood in the blood transmission tube is controlled by a swing arm machine 7 connected between the peristaltic pump 6 and the thermal desorption injector 4 to be sent into a waste liquid bottle 8;

[0028] Example 1

[0029] According to the implementation method of the present invention, the mass flow controller is set to pump 1000 sccm, the chemical dopant is acetone, the input gas flow is set to 100 sccm, the drift gas flow is set to 600 sccm, and the sample carrier gas pumping flow at the air inlet can be calculated to be 300 sccm.

[0030] Example 2

[0031] The blood transfer tube has an inner diameter of 0.6 mm and a length of 0.5 m, and the blood sampling volume is 141 μL. The peristaltic pump speed parameter is: 0.6 μL / min-100 ml / min. By adjusting the flow rate of the peristaltic pump 6, the corresponding blood collection time of different blood collection volumes can be calculated.

[0032] The above embodiments are only preferred embodiments of the present invention and are not intended to limit the implementation methods. The protection scope of the present invention shall be subject to the scope defined in the claims. Other different forms of changes or modifications may be made based on the above description. Obvious changes or modifications derived therefrom are still within the protection scope of the present invention.

Claims

1. A continuous sampling propofol blood concentration analyzer detection system, Features: It comprises an automatic continuous blood sampling device, a thermal desorption sample injector (4), an ion migration tube detector (10), an air pump (1), and a signal receiving and processing device; The ion transfer tube detector (10) is provided with an air outlet (2) connected to an air pump (1), a sample carrier gas inlet (11), a floating gas inlet (9) and a chemical dopant inlet (3), and the gas circuit system is in a pump air extraction and sampling mode; The automatic continuous blood sampling device collects blood stored in an extracorporeal blood storage chamber (5); one end of the thermal desorption injector (4) is connected to the automatic continuous blood sampling device; the other end of the thermal desorption injector (4) is connected to a sample carrier gas inlet (11) of an ion transfer tube detector (10) via a gas connection tube; The signal output end of the ion transfer tube detector (10) is connected to a signal receiving and processing device.

2. The analyzer detection system according to claim 1, Features: The automatic continuous blood sampling device comprises a peristaltic pump (6), an arm swing machine (7) and a waste liquid bottle (8); the extracorporeal blood storage chamber (5) supplies blood to a thermal desorption sample injector (4) via the peristaltic pump (6); an arm swing machine (7) is externally connected to a blood transmission tube between the peristaltic pump (6) and the thermal desorption sample injector (4); and the arm swing machine (7) is connected to the waste liquid bottle (8).

3. The analyzer detection system according to claim 1, Features: The ionization source of the ion transfer tube detector (10) is a VUV light ionization source.

4. The analyzer detection system according to claim 3, Features: The gas outlet (2), the chemical dopant inlet (3) and the sample carrier gas inlet (11) are located in an area close to the VUV light ionization source, and are the gas outlet (2), the chemical dopant inlet (3) and the sample carrier gas inlet (11) in descending order of distance from the lamp.

5. A method for detecting the blood concentration of propofol by continuous sampling, Features: The detection method utilizes the analyzer detection system described in any one of claims 1 to 4. The blood in the extracorporeal blood storage chamber (5) is collected by an automatic continuous blood sampling device. The blood sample is vaporized in the thermal desorption chamber of the thermal desorption injector (4). The gas is pumped by a vacuum pump (2) into an ion migration tube detector (10) for detection and analysis.

6. The detection method according to claim 5, Features: The blood entering the thermal desorption sample injector (4) is controlled by an arm swing machine (7), and the excess blood is discharged into a waste liquid bottle (8) as waste liquid.

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