Edible fungus strain transfer technology

Abstract

The invention provides an edible fungus strain transfer operation technology which comprises the following steps: 1, plate propagation of mother strains: selecting high-quality mycelia from stock strains, and inoculating the mycelia into a plate culture dish for propagation culture; when the flat plates are full of hyphae, unqualified hypha flat plates with color change, angle change, weak growth vigor and the like are excluded, and the qualified hypha flat plate A is selected and picked out. 2, preparing sterile water: adding purified water into a triangular flask, simultaneously adding a magnetic rotor for sealing, and cooling for later use after high-pressure sterilization; 3, preparation of mycelium homogenate: under a sterile condition, dividing the mycelia in the step A together with the culture medium into segments by using a sterile scalpel, clamping the segments into the prepared sterile water by using tweezers, placing the sterile water on a magnetic stirrer, adjusting the rotating speed, and beating the sterile water into homogenate with obvious mycelium segments; and 4, inoculation and propagation: inoculating the homogenate into a required culture medium under a sterile condition, and carrying out subsequent propagation culture according to culture condition requirements of strains.

Description

Technical Field

[0001] The present invention relates to the field of edible mushroom cultivation and production, and specifically to a transfer operation technology for edible mushroom strains. Background Art

[0002] In the cultivation process of edible mushrooms, the transfer of strains is a key step. However, there are some problems with traditional strain transfer methods. First, due to problems such as strain degeneration, color change, and angular change, the success rate of strain propagation may decrease. Second, traditional strain transfer methods often require an intermediate culture medium preparation step, which not only increases the cost of seed production but also increases the complexity of the operation steps. In addition, traditional inoculation methods require a relatively high professional level of technicians, which to a certain extent limits the popularization and application of this technology. Therefore, it is necessary to develop a new operation technology for edible mushroom strain transfer to solve the above problems. Summary of the Invention

[0003] The present invention provides an operation technology for edible mushroom strain transfer, including the following steps: Step 1: Mother culture plate propagation First, inoculate the original strain into a petri dish for propagation culture. After the mycelium fills the plate, exclude unqualified mycelium plates with color change, angular change, weak growth, etc., and select qualified mycelium plate A.

[0004] Step 2: Preparation of sterile water Add pure water and a magnetic rotor to an Erlenmeyer flask, seal it, and perform autoclaving at 121 °C for 20 - 30 minutes, then let it cool for later use B.

[0005] Step 3: Preparation of mycelium homogenate Under sterile conditions, first use a sterile scalpel to cut the mycelium in A together with the culture medium into segments, then use forceps to pick up the segments and transfer them into B. Place B on a magnetic stirrer and adjust the rotation speed to beat it into a homogenate C with obvious mycelium segments.

[0006] Step 4: Inoculation Under sterile conditions, inoculate C into the required culture medium, and perform subsequent propagation culture according to the culture conditions requirements of the strain.

[0007] 1. The mycelium segments in the homogenate have good diffusibility in the culture medium and germinate rapidly, which can effectively shorten the culture cycle of the strain or cultivation bag; 2. Propagating the strain in a petri dish in advance is beneficial to selecting degenerated, color-changed, and angular-changed strains and improving the probability of successful propagation; 3. Preparing the homogenate with sterile water can effectively save the cost of seed production without going through the intermediate culture medium preparation step; 4. The mycelium homogenate prepared by this method can be widely used for sowing and propagation of cultivation substrates such as strain bags (bottles), cultivation bags (bottles), cultivated land, etc. 5. This method is more convenient to operate compared with the traditional inoculation method, and the professional level requirements for technicians are reduced. Example

[0008] The operation process of the example is described in detail with Pleurotus ostreatus var. citrinopileatus as an example: First, inoculate the Pleurotus ostreatus var. citrinopileatus slant mother strain on 9-cm glass plates, and transfer a total of 50 plates. Cultivate at 25 °C for about 10 days until the mycelium covers the plates. Pick out the variant plates and keep 40 plates. Put 500 mL of water in a 500-mL volumetric Erlenmeyer flask, add a 3.5-cm magnetic rotor, seal it with a sealing film, and place it in an autoclave for sterilization at 121 °C for 30 min. Take it out and let it cool for later use. Mix 80% sawdust, 18% wheat bran, and 2% gypsum, add water to make a culture substrate with a moisture content of about 65%, fill it into bags and put on collars to make cultivation bags, each cultivation bag weighing about 1 kg, sterilize at 121 °C for 5 h, take it out and let it cool for later use. Under sterile conditions, use a scalpel to cut the mycelium plates into several pieces. Use sterile forceps to pick up the mycelium pieces and transfer them into the Erlenmeyer flask, one plate for each Erlenmeyer flask. Place the Erlenmeyer flask on a magnetic stirrer, and release the mycelium fragments on the mycelium blocks by vibrating and stirring to make a mycelium homogenate. After this homogenate is left standing, the agar blocks will sink to the bottom of the flask, and the liquid part is taken for inoculation. Under sterile conditions, inoculate the mycelium homogenate into the cultivation bags. One 500-mL Erlenmeyer flask can be used to transfer 20 cultivation bags. Place the inoculated cultivation bags in a cultivation room at 20 °C for cultivation. Dispersed mycelium germination can be seen within three days after inoculation, while mycelium germination only appears around the mycelium blocks in the case of inoculation with mycelium blocks under the same conditions. Specific implementation mode

[0009] The above are only the preferred embodiments of the present invention and are not used to limit the protection scope of the present invention. For those of ordinary skill in the art, various changes and modifications can be made according to the design of the present invention without departing from the principle of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.

Claims

1. An operation technique for transferring edible mushroom strains, characterized in that, this technique comprises the following steps: a. Mother culture plate propagation: First, inoculate the original strain into a petri dish for propagation culture. After the mycelium covers the plate, exclude unqualified mycelium plates with color change, angular change, weak growth, etc., and pick out qualified mycelium plates; b. Preparation of sterile water: Add pure water and a magnetic rotor into an Erlenmeyer flask. After sealing, sterilize at 121 °C under high pressure for 20 - 30 min, and let it cool for later use; c. Preparation of mycelium homogenate: Under sterile conditions, first use a sterile scalpel to cut the mycelium together with the culture medium in the qualified mycelium plate into fragments, then use forceps to pick up the fragments and transfer them into sterile water. Place the sterile water on a magnetic stirrer and adjust the rotation speed to beat it into a homogenate with obvious mycelium fragments; d. Under sterile conditions, inoculate the homogenate into the required culture medium, and carry out subsequent propagation culture according to the culture conditions requirements of the strain.

2. The operation technique for transferring edible mushroom strains according to claim 1, characterized in that, this technique can be used for preparing original strains and cultivated strains.

3. The operation technique for transferring edible mushroom strains according to claims 1 and 2, characterized in that, the advantages of this technique include: the mycelium fragments in the homogenate have good diffusibility in the culture medium and germinate rapidly, which can effectively shorten the culture cycle of the strain or cultivation bag; propagating the strain in a petri dish in advance is beneficial to selecting degenerated, color - changed, and angular - changed strains, and improving the probability of successful propagation; preparing the homogenate through sterile water without going through the intermediate culture medium preparation link can effectively save the cost of seed production; the mycelium homogenate prepared by this method can be widely used for seeding and propagation of cultivation substrates such as strain bags (bottles), cultivation bags (bottles), cultivated land, etc.; this method is more convenient to operate compared with the traditional inoculation method, and the professional level requirements for technicians are reduced.