GK rat breeding feed for type II diabetes mellitus model

Abstract

The invention discloses a type II diabetes mellitus model GK rat breeding feed which is used for feeding GK rats in a breeding stage after weaning, so that the GK rats can 100% reach the standard of establishment of a type II diabetes mellitus model with random blood sugar greater than or equal to 11.1 mmol / L when the rats are 12 weeks old, and the modeling time is 2-4 weeks earlier than that when the rats are fed with general experimental rat growth and reproduction feed or high-protein feed; the fluctuation range of the blood glucose level is smaller, and standardization of establishment of a type II diabetes model GK rat model and development of related scientific researches are facilitated; in order to solve the technical problem, the technical scheme adopted by the invention is as follows: the breeding feed for the type II diabetes model GK rats comprises the following components: 7 parts of fish meal, 7 parts of chicken meal, 67 parts of soybean meal, 63 parts of corn, 88 parts of wheat, 7 parts of soybean oil, 28 parts of lard, 7 parts of beer yeast powder, 7 parts of bran, 7 parts of milk powder, 18 parts of casein, 18 parts of cane sugar, 11 parts of maltodextrin and 18 parts of a rat premix. The invention can be widely applied to the field of animal model feeds.

Description

Technical Field

[0001] The present invention relates to a breeding feed for GK rats with type II diabetes, belonging to the technical field of animal model feeds. Background Art

[0002] GK rats were obtained in Sendai, Japan in 1973 by Goto, Kakizaki et al. through inbreeding and repeating several generations of Wistar rats with the upper limit of glucose tolerance (IGT). The weaning period is about 28 days. Female rats reach sexual maturity at about 8 - 10 weeks, and male rats at about 10 - 12 weeks. The blood glucose level of GK rats is normal at the neonatal stage and gradually develops into overt diabetes during the breeding period, showing mild fasting hyperglycemia, obvious postprandial hyperglycemia, hyperinsulinemia, insulin resistance, impaired insulin secretion stimulated by glucose, without ketosis. It is one of the best models for studying the pathogenesis of type II diabetes and insulin resistance, belonging to non-insulin-dependent non-obese spontaneous type II diabetic model rats.

[0003] Since GK rats are congenital spontaneous diabetic model rats, according to the existing technology, during the breeding period, experimental rat growth and reproduction feed (crude protein ≥ 20%, crude fat ≥ 4%) or high-protein feed (crude protein ≥ 25%, crude fat ≥ 4%) is generally used to feed GK rats. Under this feeding condition, adult GK rats are prone to have a relatively large age at onset, and develop type II diabetes at about 14 - 16 weeks of age. In the existing technology, there are also cases where high-fat experimental rat feed with a fat energy supply ratio exceeding 35% is used to feed GK rats after weaning. Under this feeding condition, GK rats are prone to have unstable blood glucose values and too rapid development of the diabetes course, affecting the development of relevant scientific research experiments. Summary of the Invention

[0004] The present invention overcomes the deficiencies of the prior art and provides a breeding feed for GK rats with type II diabetes. As an experimental feed dedicated to the breeding stage of GK rats with type II diabetes after weaning, it is used to feed GK rats in the breeding stage after weaning, enabling 100% of GK rats to meet the standard for establishing a type II diabetes model with a random blood glucose ≥ 11.1 mmol / L at 12 weeks of age, which is 2 - 4 weeks earlier than when feeding general experimental rat growth and reproduction feed (crude protein ≥ 20%, crude fat ≥ 4%) or high-protein feed (crude protein ≥ 25%, crude fat ≥ 4%); at the same time, the stability of blood glucose values is better. The random blood glucose at 12 weeks of age is 13.01 ± 0.91 mmol / L, while the random blood glucose at 12 weeks of age for GK rats fed high-protein feed after weaning is 10.64 ± 1.65 mmol / L, and the random blood glucose at 12 weeks of age for GK rats fed a high-fat experimental rat feed with a 35% fat energy supply ratio after weaning is 11.94 ± 2.58 mmol / L. The GK rats fed the special breeding feed of the present invention have a smaller range of blood glucose value fluctuations, which is more conducive to the standardization of the establishment of the type II diabetes model of GK rats and the development of related scientific research.

[0005] To solve the above technical problems, the technical solution adopted by the present invention is: a breeding feed for GK rats with type II diabetes, comprising the following components: 5 - 35 parts of fish meal, 2 - 20 parts of chicken meal, 50 - 80 parts of soybean meal, 55 - 90 parts of corn, 60 - 105 parts of wheat, 2 - 20 parts of soybean oil, 15 - 40 parts of lard, 5 - 20 parts of brewer's yeast powder, 5 - 18 parts of wheat bran, 5 - 16 parts of milk powder, 5 - 25 parts of casein, 5 - 25 parts of sucrose, 5 - 20 parts of maltodextrin, 5 - 25 parts of rat premix.

[0006] Furthermore, 7 parts of fish meal, 7 parts of chicken meal, 63 - 77 parts of soybean meal, 63 - 77 parts of corn, 91 - 105 parts of wheat, 7 parts of soybean oil, 7 - 36 parts of lard, 7 parts of brewer's yeast powder, 7 parts of wheat bran, 7 parts of milk powder, 7 - 21 parts of casein, 7 - 25 parts of sucrose, 11 parts of maltodextrin, 18 parts of rat premix.

[0007] Preferably, it comprises the following components: 7 parts of fish meal, 7 parts of chicken meal, 67 parts of soybean meal, 63 parts of corn, 88 parts of wheat, 7 parts of soybean oil, 28 parts of lard, 7 parts of brewer's yeast powder, 7 parts of wheat bran, 7 parts of milk powder, 18 parts of casein, 18 parts of sucrose, 11 parts of maltodextrin, 18 parts of rat premix.

[0008] Furthermore, 35 parts of fish meal, 20 parts of chicken meal, 80 parts of soybean meal, 90 parts of corn, 105 parts of wheat, 20 parts of soybean oil, 40 parts of lard, 20 parts of brewer's yeast powder, 18 parts of wheat bran, 16 parts of milk powder, 25 parts of casein, 25 parts of sucrose, 20 parts of maltodextrin, 25 parts of rat premix.

[0009] Furthermore, 5 parts of fish meal, 2 parts of chicken meal, 50 parts of soybean meal, 55 parts of corn, 60 parts of wheat, 2 parts of soybean oil, 15 parts of lard, 5 parts of beer yeast powder, 5 parts of wheat bran, 5 parts of milk powder, 5 parts of casein, 5 parts of sucrose, 5 parts of maltodextrin, and 5 parts of rat premix.

[0010] Furthermore, it is used for feeding GK rats in the post - weaning growing stage.

[0011] Furthermore, the time for the establishment of the type 2 diabetes model in GK rats is shortened from 14 - 16 weeks to 12 weeks.

[0012] Furthermore, the following method is used for the modeling experiment: Randomly select 60 male GK rats at 3 weeks old and randomly divide them into 6 groups, with 10 rats in each group, namely Group 1, Group 2, Group 3, Group 4, Group 5, and the control group; Groups 1 to 5 are fed with type 2 diabetes model GK rat growing feed with different formulas, and the control group is given the high - protein rat growth and reproduction compound feed P1104 - F35 provided by Shanghai Pruton Biotechnology Co., Ltd. They are fed from 3 weeks to 12 weeks. Starting from 9 weeks, they are fasted for 24 hours, and the fasting blood glucose is measured with a blood glucose tester until 12 weeks. Before measuring the random blood glucose, they are not fasted, and it is measured at 9:00 the next day until 12 weeks; Calculation of the modeling rate: Random blood glucose ≥ 11.1 mmol / L is considered hyperglycemic GK rats, Calculate the modeling rate = number of hyperglycemic GK rats / total number of GK rats × 100%.

[0013] The beneficial effects of the present invention compared with the prior art are as follows: Compared with the prior art, feeding the special growing feed for GK rats of the present invention can make 100% of GK rats reach the standard for the establishment of the type 2 diabetes model with random blood glucose ≥ 11.1 mmol / L at 12 weeks old, and can shorten the time for the establishment of the type 2 diabetes model in GK rats from 14 - 16 weeks to 12 weeks. Feeding the special growing feed for GK rats of the present invention can make the blood glucose value of GK rats more stable, with a smaller fluctuation range, which is more conducive to the standardization of the establishment of the type 2 diabetes model in GK rats and the development of related scientific research. Specific Embodiments

[0014] The present invention will be further elaborated below in combination with specific embodiments. Embodiment

[0015] A breeding feed for GK rats with type II diabetes model provided by the present invention comprises the following components: 7 parts of fish meal, 7 parts of chicken meal, 77 parts of soybean meal, 77 parts of corn, 105 parts of wheat, 7 parts of soybean oil, 7 parts of lard, 7 parts of beer yeast powder, 7 parts of wheat bran, 7 parts of milk powder, 7 parts of casein, 7 parts of sucrose, 11 parts of maltodextrin, and 18 parts of rat premix. Example

[0016] A breeding feed for GK rats with type II diabetes model provided by the present invention comprises the following components: 7 parts of fish meal, 7 parts of chicken meal, 74 parts of soybean meal, 74 parts of corn, 98 parts of wheat, 7 parts of soybean oil, 14 parts of lard, 7 parts of beer yeast powder, 7 parts of wheat bran, 7 parts of milk powder, 7 parts of casein, 11 parts of sucrose, 11 parts of maltodextrin, and 18 parts of rat premix. Example

[0017] A breeding feed for GK rats with type II diabetes model provided by the present invention comprises the following components: 7 parts of fish meal, 7 parts of chicken meal, 70 parts of soybean meal, 70 parts of corn, 91 parts of wheat, 7 parts of soybean oil, 21 parts of lard, 7 parts of beer yeast powder, 7 parts of wheat bran, 7 parts of milk powder, 14 parts of casein, 14 parts of sucrose, 11 parts of maltodextrin, and 18 parts of rat premix. Example

[0018] A breeding feed for GK rats with type II diabetes model provided by the present invention comprises the following components: 7 parts of fish meal, 7 parts of chicken meal, 67 parts of soybean meal, 63 parts of corn, 88 parts of wheat, 7 parts of soybean oil, 28 parts of lard, 7 parts of beer yeast powder, 7 parts of wheat bran, 7 parts of milk powder, 18 parts of casein, 18 parts of sucrose, 11 parts of maltodextrin, and 18 parts of rat premix. Example

[0019] A breeding feed for GK rats with type II diabetes model provided by the present invention comprises the following components: 7 parts of fish meal, 7 parts of chicken meal, 63 parts of soybean meal, 65 parts of corn, 81 parts of wheat, 7 parts of soybean oil, 36 parts of lard, 7 parts of beer yeast powder, 7 parts of wheat bran, 7 parts of milk powder, 21 parts of casein, 25 parts of sucrose, 11 parts of maltodextrin, and 18 parts of rat premix.

[0020] In Examples 1 to 5, the fat content increases from 6% to 14% to explore which feed with what fat content is most suitable for the growth period of GK rats. The rat premix includes calcium hydrogen phosphate, calcium carbonate, salt, lysine, methionine, compound vitamins, compound minerals, and choline chloride in a mass ratio of 28 - 35:22 - 28:4 - 10:9 - 20:4 - 10:1 - 5:2 - 5:2 - 5.

[0021] The present invention uses the same production process to prepare the breeding feed for type II diabetic model GK rats in the above 5 groups of examples, and the following method is used for the modeling experiment:

[0022] Blood glucose measurement: Randomly select 60 male GK rats at 3 weeks of age, and randomly divide them into 6 groups, with 10 rats in each group, namely Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, and control group. The rats in Example 1 to Example 5 groups were fed the breeding feed for type II diabetic model GK rats in Examples 1 to 5 respectively, and the control group was given the high-protein rat growth and reproduction compound feed P1104-F35 provided by Shanghai Pruteng Biotechnology Co., Ltd. (crude protein ≥ 25%, crude fat ≥ 4%). They were fed from 3 weeks to 12 weeks. Starting from 9 weeks, they were fasted for 24 hours, and the fasting blood glucose was measured with a blood glucose tester until 12 weeks. Random blood glucose was measured without fasting before the next day at 9:00 until 12 weeks.

[0023] Calculation of the successful modeling rate: Statistical analysis of the successful modeling rate: Count the number of rats with random blood glucose ≥ 11.1 mmol / L, and calculate the successful modeling rate (number of hyperglycemic GK rats / total number of GK rats × 100%).

[0024] The specific results are shown in Tables 1, 2, 3, and 4 below.

[0025] In the table, random blood glucose refers to the blood glucose at any time regardless of the meal time, which can be measured at any time before or after a meal.

[0026] The normal value of random blood glucose is less than 7.8 mmol / L. If the random blood glucose is greater than 11.1 mmol / L and accompanied by the typical three more and one less symptoms of diabetes, diabetes can be diagnosed. If not accompanied by the typical symptoms of diabetes, another blood glucose measurement at a different time is required to confirm or rule out the diagnosis of diabetes. Random blood glucose between 7.8 - 11.1 mmol / L is diagnosed as impaired glucose tolerance, which belongs to the pre-diabetes stage.

[0027] Among them: The average value refers to the average value of the random blood glucose tests of 10 rats in each group of experiments.

[0028] The standard deviation is a statistical term, also known as the standard deviation, which refers to a standard for measuring the dispersion degree of data distribution. The larger the standard deviation, the worse the blood glucose stability, and vice versa.

[0029] The number of modeled rats refers to the number of GK rats whose random blood glucose is greater than 11.1 mmol / L, which is the diagnostic criterion for diabetes.

[0030] The modeling rate = the number of hyperglycemic GK rats / the total number of GK rats × 100%, which reflects the proportion of GK rats that can form type II diabetes models.

[0031] The results of the above embodiments show that: As can be seen from Table 1, Table 2, Table 3, and Table 4, the modeling rate of Group 4 in Example 4 is the highest. The modeling rate reached 60% at 9 weeks, while the highest rate of the other groups only reached 30%. And by 12 weeks, the modeling rate reached 100%, while the modeling rate of the control group fed with the conventional growth and reproduction feed for rats and mice was still only 10% at this time.

[0032] As can be seen from Table 4, the standard deviation of random blood glucose in Group 4 of Example 4 at 12 weeks of age was 0.91 mmol / L. Although the feed fed to Group 5 in Example 5 had a higher fat content, the blood glucose stability was not good, and the standard deviation of random blood glucose at 12 weeks of age was 2.85 mmol / L.

[0033] From the above analysis, it can be seen that using the feed for breeding GK rats with type II diabetes models of the present invention can effectively improve the modeling rate, shorten the modeling time, improve the stability of blood glucose values during the growth period of GK rats, and is conducive to the standardization of the establishment of type II diabetes model GK rats and the development of related scientific research.

[0034] The present invention has been described in detail above in conjunction with the embodiments. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art, various changes can be made without departing from the gist of the present invention.

Claims

1. A feed for breeding type Ⅱ diabetes model GK rats, characterized in that: It contains the following components: 5-35 parts of fish meal, 2-20 parts of chicken meal, 50-80 parts of soybean meal, 55-90 parts of corn, 60-105 parts of wheat, 2-20 parts of soybean oil, 15-40 parts of lard, 5-20 parts of brewer's yeast powder, 5-18 parts of bran, 5-16 parts of milk powder, 5-25 parts of casein, 5-25 parts of sucrose, 5-20 parts of maltodextrin, and 5-25 parts of mouse premix.

2. A type II diabetes model GK rat breeding feed according to claim 1, characterized in that: It contains the following components: 7 parts of fish meal, 7 parts of chicken meal, 63-77 parts of soybean meal, 63-77 parts of corn, 91-105 parts of wheat, 7 parts of soybean oil, 7-36 parts of lard, 7 parts of brewer's yeast powder, 7 parts of bran, 7 parts of milk powder, 7-21 parts of casein, 7-25 parts of sucrose, 11 parts of maltodextrin, and 18 parts of mouse premix.

3. A type II diabetes model GK rat breeding feed according to claim 2, characterized in that: It contains the following components: 7 parts of fish meal, 7 parts of chicken meal, 67 parts of soybean meal, 63 parts of corn, 88 parts of wheat, 7 parts of soybean oil, 28 parts of lard, 7 parts of brewer's yeast powder, 7 parts of bran, 7 parts of milk powder, 18 parts of casein, 18 parts of sucrose, 11 parts of maltodextrin, and 18 parts of mouse premix.

4. A type II diabetes model GK rat breeding feed according to claim 1 or 2, characterized in that: Used for feeding GK rats in the growing stage after weaning.

5. A type II diabetes model GK rat breeding feed according to claim 1 or 2, characterized in that: The time required to establish the type 2 diabetes model in GK rats was shortened from 14-16 weeks to 12 weeks.

6. A type II diabetes model GK rat breeding feed according to claim 1 or 2, characterized in that: The modeling experiment was carried out using the following method: Sixty 3-week-old GK rats were randomly selected and divided into 6 groups, 10 rats in each group, namely group 1, group 2, group 3, group 4, group 5, and control group. Groups 1 to 5 were fed with different formulas of type Ⅱ diabetes model GK rat breeding feed, and the control group was fed with high-protein rat growth and reproduction feed P1104-F35 provided by Shanghai Proton Biotechnology Co., Ltd. from 3 weeks to 12 weeks. They were fasted for 24 hours from 9 weeks. Fasting blood sugar was measured by blood glucose tester until 12 weeks. There was no fasting before random blood sugar measurement, and it was measured at 9:00 the next day until 12 weeks. Modeling rate calculation: Random blood glucose ≥ 11.1mmol / L is considered as hyperglycemic GK mice. The calculated model success rate = number of hyperglycemic GK mice / total number of GK mice × 100%.

Images