Camellia seed extract with oil control and decontamination functions as well as preparation method and application of camellia seed extract

Abstract

The invention belongs to the technical field of daily chemicals, and particularly relates to a camellia seed extract with oil control and decontamination functions as well as a preparation method and application thereof, and the international IPC classification number of the camellia seed extract is A61K8. The camellia seed extract is prepared by crushing camellia seeds, decocting, carrying out primary enzymolysis on cellulase, carrying out secondary enzymolysis on ginger protease, fermenting bacillus licheniformis CICC 10103 and lactobacillus plantarum CICC 21834, further extracting with an ethanol water solution, concentrating and drying, and has excellent properties of decontamination, oil control, moisturizing and the like. The cosmetic prepared from the camellia seed extract is low in irritation and high in safety, and has good effects of removing dirt, controlling oil and increasing water content of skin.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemicals, and its international IPC classification number is A61 K8. It specifically relates to a camellia seed extract with oil control and decontamination functions, and a preparation method and application thereof. Background Art

[0002] Camellia seeds are rich in water, crude fat, starch, crude protein, tea seed polysaccharides, polyphenols, flavonoids, tea saponins, crude fiber, tannins, polypeptides, squalene, vitamins, amino acids and other active ingredients.

[0003] Tea saponin in camellia seed extract is a type of saponin, a glycoside compound composed of four parts: sapogenin, glycosome, uronic acid and organic acid. Tea saponin has good water solubility and excellent surface activity. It can replace chemical surfactants to clean the scalp follicles and balance the oil secretion of the scalp. Tea saponin has low surface tension, good hydrophilic and lipophilic effects, and good dispersion, foaming and decontamination properties. It is a good natural surfactant that is neutral, green, safe and low in irritation. Adding tea saponin to shampoo products can have both shampooing and hair care functions.

[0004] In addition, camellia seeds are rich in nutrients such as amino acids, peptides, polysaccharides, flavonoids, and polyphenols, which give camellia seed extracts antioxidant, anti-inflammatory, and antibacterial effects, which can protect the skin from damage by the external environment and delay aging; they can also nourish the scalp follicles, promote scalp health, and enhance the growth and health of hair; they can also nourish the hair roots, promote hair growth, and make the hair more abundant and soft; they also have a strong moisturizing effect, which can delay scalp aging and nourish and strengthen hair. The combination of the active ingredients in camellia seeds can effectively remove dandruff, prevent hair loss, protect scalp health, control oil and clean the scalp, remove greasiness, provide nourishment for the scalp, and can deeply clean the scalp, remove dirt and oil, and keep the scalp fresh.

[0005] Currently, there are relatively few research reports on improving the oil control and cleansing functions of camellia seed extract. Therefore, it is particularly important to provide an extraction method that can improve the oil control and cleansing effects of camellia seed extract and apply it in cosmetics. Summary of the invention

[0006] In view of the above technical problems, the inventors proposed the technical solution of the present invention, which includes a camellia seed extract with oil control and decontamination functions, and a preparation method and application thereof.

[0007] The technical solution of the present invention is specifically as follows:

[0008] A method for preparing a camellia seed extract having oil-controlling and decontamination-removing functions comprises the following steps:

[0009] S1: crushing camellia seeds to obtain camellia seed powder, adding water to boil, cooling, and obtaining product 1;

[0010] S2: adding cellulase to product 1, performing enzymolysis for the first time, and then killing the enzyme; then adding ginger protease, performing enzymolysis for the second time, and then killing the enzyme, to obtain product 2;

[0011] S3: adding glucose and nitrogen source to product 2, mixing, then adding fermentation bacteria, fermenting, sterilizing, and obtaining product 3;

[0012] S4: adding ethanol to the product three, adjusting the ethanol concentration, heating and stirring, filtering, and obtaining a filtrate, which is the product four;

[0013] S5: The product 4 is concentrated under reduced pressure and dried to obtain the camellia seed extract.

[0014] Furthermore, in S1, the mass of water added is 10-40 times that of the camellia seed powder.

[0015] Furthermore, in S1, the amount of cellulase added is 500-800u / g product one.

[0016] Furthermore, the temperature and time of the first enzymatic hydrolysis are 40-60° C. and 2-5 hours.

[0017] Furthermore, in S2, the amount of ginger protease added is 400-700 U / g product one.

[0018] Furthermore, the temperature and time of the second enzymatic hydrolysis are 40-60° C. and 2-5 hours.

[0019] Furthermore, in S3, the added amounts of glucose and nitrogen source are 1-3% and 0.002-0.01% of the mass of the second product, respectively.

[0020] Further, the nitrogen source includes at least one of potassium dihydrogen phosphate and dipotassium hydrogen phosphate.

[0021] Furthermore, the fermentation temperature is 30-40°C and the fermentation time is 120-200 hours.

[0022] Furthermore, in S3, the fermentation bacteria dosage was 3.5×10 9 -8.5×10 9 cfu / g product two.

[0023] Further, the fermentation bacteria consist of Bacillus licheniformis CICC 10103 and Bacillus plantarum CICC 21834 in a quantitative ratio of 1:0.8-1.3.

[0024] Furthermore, in S4, the ethanol mass concentration is adjusted to 15-35%, and the heating and stirring temperature and time are 40-60° C. and 4-10 hours.

[0025] Further, in S4, the product is concentrated under reduced pressure to a mass of 8-15% of the mass of the product four.

[0026] Further, in S4, drying is performed until the water content is less than 5 wt%.

[0027] A camellia seed extract with oil-controlling and decontamination-removing functions prepared according to the preparation method.

[0028] A use of the camellia seed extract with oil control and dirt removal function in the preparation of cosmetics, wherein the mass percentage of the camellia seed extract with oil control and dirt removal function in the cosmetics is 0.5-10%.

[0029] A cosmetic comprises, by mass percentage, 0.5-10% of the camellia seed extract, 0.1-2% of rosemary extract, 0.1-2% of green tea extract, 0.2-2% of witch hazel extract, 1-5% of soapberry saponin, 1-5% of alkyl glycoside, 1-3% of thickener, 3-8% of polyol, 0.1-0.5% of sodium citric acid, 0.08-0.3% of preservative and the balance of water.

[0030] Furthermore, in the cosmetic, the thickener includes at least one of a polymer or a plant gum.

[0031] Furthermore, in the cosmetic, the polyol includes at least one of ethylene glycol, propylene glycol, butylene glycol and glycerol.

[0032] A method for preparing cosmetics comprises the steps of stirring and mixing the raw materials of the cosmetics, aging, sterilizing and filling.

[0033] Beneficial effects of the present invention:

[0034] The invention comprises the following steps: crushing camellia seeds, boiling, performing a first enzymolysis with cellulase, performing a second enzymolysis with ginger protease, fermenting with Bacillus licheniformis CI CC 10103 and Bacillus plantarum CI CC 21834, further extracting with an ethanol aqueous solution, concentrating and drying, thereby preparing a camellia seed extract having excellent properties such as cleansing, oil control and moisturizing.

[0035] The camellia seed extract obtained by the specific preparation method of the present invention has excellent dirt removal, oil control and skin moisturizing effects.

[0036] The invention adopts ginger protease enzymatic hydrolysis to prepare camellia seed extract, which has better dirt removal, oil control and moisturizing effects.

[0037] The present invention adopts Bacillus licheniformis CI CC 10103 and Bacillus plantarum CI CC 21834 to jointly ferment to prepare camellia seed extract, which has better effect than using only one of them; and when the number of fermented bacteria is kept unchanged, the oil control and moisturizing properties obtained by fermentation of the two are better; when the two are jointly fermented, a synergistic relationship is generated.

[0038] The fermentation bacteria of the present invention are composed of Bacillus licheniformis CI CC 10103 and Bacillus plantarum CI CC 21834 in a quantity ratio of 1:0.8-1.3 for fermentation. Compared with fermentation with bacteria combinations in other ratios, the obtained camellia seed extract has better effect.

[0039] The present invention further extracts the fermented product with ethanol aqueous solution, which can increase the decontamination, oil control and moisturizing effects of the extract, and when the concentration of the ethanol aqueous solution is 15-35wt%, the extraction effect is best.

[0040] The cosmetics prepared by using the camellia seed extract of the present invention have low irritation, high safety, and good effects of removing dirt, controlling oil and increasing the moisture content of the skin. DETAILED DESCRIPTION

[0041] The present invention will be described below in conjunction with specific embodiments, and various effects of the present invention will be more clearly presented. It should be understood by those skilled in the art that these specific embodiments are used to illustrate the present invention, rather than to limit the present invention.

[0042] (I) Preparation of products

[0043] Cellulase: Product No. C766286, Aladdin reagent. Zingiberin: Product No. YXL2203, Shanghai Yingxin Laboratory Equipment Co., Ltd. Neutral protease: Product No. D195752, Aladdin reagent.

[0044] 1. Preparation of Camellia Seed Extract

[0045] Extract 1:

[0046] The preparation method comprises the following steps:

[0047] S1: Crush camellia seeds to obtain camellia seed powder, add 32.5 times the mass of water, boil and cool to room temperature to obtain product 1;

[0048] S2: adding 600u / g of cellulase to product one, performing the first enzymolysis at 50°C and 60rpm for 3.5 hours, and then inactivating the enzyme; then adding 500U / g of ginger protease to product one, performing the second enzymolysis at 52°C and 70rpm for 3.7 hours, and then inactivating the enzyme to obtain product two;

[0049] S3: Add 2.2% of glucose and 0.0075% of potassium dihydrogen phosphate, a nitrogen source, to product 2, mix well, then add fermentation bacteria, stir at 35°C and 40rpm for anaerobic fermentation for 185 hours, sterilize, and obtain product 3; wherein, the amount of fermentation bacteria is 6.80×10 9 cfu / g product 2; the fermentation bacteria consisted of Bacillus licheniformis CICC 10103 and Bacillus plantarum CICC 21834 in a quantitative ratio of 1:1;

[0050] S4: Add ethanol to the product three to adjust the ethanol mass concentration to 20.5%, heat at 50°C, stir at 80 rpm for 6 hours, filter, and obtain a filtrate, which is the product four;

[0051] S5: The product 4 is concentrated under reduced pressure at 55° C. and 0.006 MPa to 10.2% of the mass of the product 4, and then freeze-dried at -45° C. to a water content of 2.76 wt %, to obtain the camellia seed extract.

[0052] Extract 2:

[0053] The preparation method comprises the following steps:

[0054] S1: Crush camellia seeds to obtain camellia seed powder, add 35 times the mass of water, boil and cool to room temperature to obtain product 1;

[0055] S2: Add 650u / g of cellulase to product one, stir at 52°C and 60rpm for the first enzymolysis for 3.2 hours, and then inactivate the enzyme; then add 480U / g of ginger protease to product one, stir at 50°C and 70rpm for the second enzymolysis for 4 hours, and inactivate the enzyme to obtain product two;

[0056] S3: Add 2.3% glucose and 0.007% potassium dihydrogen phosphate (nitrogen source) by weight to product 2, mix well, then add fermentation bacteria, stir at 36°C and 40 rpm for anaerobic fermentation for 180 hours, sterilize, and obtain product 3; wherein, the amount of fermentation bacteria is 6.85×10 9 cfu / g product 2; the fermentation bacteria consisted of Bacillus licheniformis CICC 10103 and Bacillus plantarum CICC 21834 in a quantitative ratio of 1:1.3;

[0057] S4: Add ethanol to the product three to adjust the ethanol mass concentration to 25%, heat at 52°C, stir at 80 rpm for 5.8 hours, filter, and obtain a filtrate, which is the product four;

[0058] S5: The product 4 is concentrated under reduced pressure at 55° C. and 0.006 MPa to 12.4% of the mass of the product 4, and then freeze-dried at -45° C. to a water content of 2.69 wt %, to obtain the camellia seed extract.

[0059] Extract 3:

[0060] The preparation method comprises the following steps:

[0061] S1: Crush camellia seeds to obtain camellia seed powder, add 32.5 times the mass of water, boil and cool to room temperature to obtain product 1;

[0062] S2: Add 600u / g of cellulase to product one, perform the first enzymolysis at 50°C and 60rpm for 3.5 hours, and then inactivate the enzyme; then add 500U / g of neutral protease to product one, perform the second enzymolysis at 52°C and 70rpm for 3.7 hours, and then inactivate the enzyme to obtain product two;

[0063] S3: Add 2.2% of glucose and 0.0075% of potassium dihydrogen phosphate, a nitrogen source, to product 2, mix well, then add fermentation bacteria, stir at 35°C and 40rpm for anaerobic fermentation for 185 hours, sterilize, and obtain product 3; wherein, the amount of fermentation bacteria is 6.80×10 9 cfu / g product 2; the fermentation bacteria consisted of Bacillus licheniformis CICC 10103 and Bacillus plantarum CICC 21834 in a quantitative ratio of 1:1;

[0064] S4: Add ethanol to the product three to adjust the ethanol mass concentration to 20.5%, heat at 50°C, stir at 80 rpm for 6 hours, filter, and obtain a filtrate, which is the product four;

[0065] S5: The product 4 is concentrated under reduced pressure at 55° C. and 0.006 MPa to 10.2% of the mass of the product 4, and then freeze-dried at -45° C. to a water content of 2.76 wt %, to obtain the camellia seed extract.

[0066] Extract 4:

[0067] The preparation method comprises the following steps:

[0068] S1: Crush camellia seeds to obtain camellia seed powder, add 32.5 times the mass of water, boil and cool to room temperature to obtain product 1;

[0069] S2: adding 600u / g of cellulase to product one, performing the first enzymolysis at 50°C and 60rpm for 3.5 hours, and then inactivating the enzyme; then adding 500U / g of ginger protease to product one, performing the second enzymolysis at 52°C and 70rpm for 3.7 hours, and then inactivating the enzyme to obtain product two;

[0070] S3: Add ethanol to the product 2 to adjust the ethanol mass concentration to 20.5%, heat at 50°C, stir at 80 rpm for 6 hours, filter, and obtain a filtrate, which is the product 3;

[0071] S5: The product three is concentrated under reduced pressure at 55° C. and 0.006 MPa to 10.2% of the mass of the product four, and then freeze-dried at -45° C. to a water content of 2.76 wt %, to obtain the camellia seed extract.

[0072] Extract 5:

[0073] The preparation method comprises the following steps:

[0074] S1: Crush camellia seeds to obtain camellia seed powder, add 32.5 times the mass of water, boil and cool to room temperature to obtain product 1;

[0075] S2: adding 600u / g of cellulase to product one, performing the first enzymolysis at 50°C and 60rpm for 3.5 hours, and then inactivating the enzyme; then adding 500U / g of ginger protease to product one, performing the second enzymolysis at 52°C and 70rpm for 3.7 hours, and then inactivating the enzyme to obtain product two;

[0076] S3: Add 2.2% of glucose and 0.0075% of potassium dihydrogen phosphate, a nitrogen source, to product 2, mix well, then add fermentation bacteria, stir at 35°C and 40rpm for anaerobic fermentation for 185 hours, sterilize, and obtain product 3; wherein, the amount of fermentation bacteria is 6.80×10 9 cfu / g product 2; the fermentation bacteria is Bacillus licheniformis CICC 10103;

[0077] S4: Add ethanol to the product three to adjust the ethanol mass concentration to 20.5%, heat at 50°C, stir at 80 rpm for 6 hours, filter, and obtain a filtrate, which is the product four;

[0078] S5: The product 4 is concentrated under reduced pressure at 55° C. and 0.006 MPa to 10.2% of the mass of the product 4, and then freeze-dried at -45° C. to a water content of 2.76 wt %, to obtain the camellia seed extract.

[0079] Extract VI:

[0080] The preparation method comprises the following steps:

[0081] S1: Crush camellia seeds to obtain camellia seed powder, add 32.5 times the mass of water, boil and cool to room temperature to obtain product 1;

[0082] S2: adding 600u / g of cellulase to product one, performing the first enzymolysis at 50°C and 60rpm for 3.5 hours, and then inactivating the enzyme; then adding 500U / g of ginger protease to product one, performing the second enzymolysis at 52°C and 70rpm for 3.7 hours, and then inactivating the enzyme to obtain product two;

[0083] S3: Add 2.2% of glucose and 0.0075% of potassium dihydrogen phosphate, a nitrogen source, to product 2, mix well, then add fermentation bacteria, stir at 35°C and 40rpm for anaerobic fermentation for 185 hours, sterilize, and obtain product 3; wherein, the amount of fermentation bacteria is 6.80×10 9 cfu / g product 2; the fermentation bacteria is composed of Lactobacillus plantarum CICC 21834;

[0084] S4: Add ethanol to the product three to adjust the ethanol mass concentration to 20.5%, heat at 50°C, stir at 80 rpm for 6 hours, filter, and obtain a filtrate, which is the product four;

[0085] S5: The product 4 is concentrated under reduced pressure at 55° C. and 0.006 MPa to 10.2% of the mass of the product 4, and then freeze-dried at -45° C. to a water content of 2.76 wt %, to obtain the camellia seed extract.

[0086] Extract 7:

[0087] The preparation method comprises the following steps:

[0088] S1: Crush camellia seeds to obtain camellia seed powder, add 32.5 times the mass of water, boil and cool to room temperature to obtain product 1;

[0089] S2: adding 600u / g of cellulase to product one, performing the first enzymolysis at 50°C and 60rpm for 3.5 hours, and then inactivating the enzyme; then adding 500U / g of ginger protease to product one, performing the second enzymolysis at 52°C and 70rpm for 3.7 hours, and then inactivating the enzyme to obtain product two;

[0090] S3: Add 2.2% of glucose and 0.0075% of potassium dihydrogen phosphate, a nitrogen source, to product 2, mix well, then add fermentation bacteria, stir at 35°C and 40rpm for anaerobic fermentation for 185 hours, sterilize, and obtain product 3; wherein, the amount of fermentation bacteria is 6.80×10 9 cfu / g product 2; the fermentation bacteria consisted of Bacillus licheniformis CICC 10103 and Bacillus plantarum CICC 21834 in a quantitative ratio of 1:0.2;

[0091] S4: Add ethanol to the product three to adjust the ethanol mass concentration to 20.5%, heat at 50°C, stir at 80 rpm for 6 hours, filter, and obtain a filtrate, which is the product four;

[0092] S5: The product 4 is concentrated under reduced pressure at 55° C. and 0.006 MPa to 10.2% of the mass of the product 4, and then freeze-dried at -45° C. to a water content of 2.76 wt %, to obtain the camellia seed extract.

[0093] Extract Eight:

[0094] The preparation method comprises the following steps:

[0095] S1: Crush camellia seeds to obtain camellia seed powder, add 32.5 times the mass of water, boil and cool to room temperature to obtain product 1;

[0096] S2: adding 600u / g of cellulase to product one, performing the first enzymolysis at 50°C and 60rpm for 3.5 hours, and then inactivating the enzyme; then adding 500U / g of ginger protease to product one, performing the second enzymolysis at 52°C and 70rpm for 3.7 hours, and then inactivating the enzyme to obtain product two;

[0097] S3: Add 2.2% of glucose and 0.0075% of potassium dihydrogen phosphate, a nitrogen source, to product 2, mix well, then add fermentation bacteria, stir at 35°C and 40rpm for anaerobic fermentation for 185 hours, sterilize, and obtain product 3; wherein, the amount of fermentation bacteria is 6.80×10 9 cfu / g product 2; the fermentation bacteria consisted of Bacillus licheniformis CICC 10103 and Bacillus plantarum CICC 21834 in a quantitative ratio of 1:5;

[0098] S4: Add ethanol to the product three to adjust the ethanol mass concentration to 20.5%, heat at 50°C, stir at 80 rpm for 6 hours, filter, and obtain a filtrate, which is the product four;

[0099] S5: The product 4 is concentrated under reduced pressure at 55° C. and 0.006 MPa to 10.2% of the mass of the product 4, and then freeze-dried at -45° C. to a water content of 2.76 wt %, to obtain the camellia seed extract.

[0100] Extract Nine:

[0101] The preparation method comprises the following steps:

[0102] S1: Crush camellia seeds to obtain camellia seed powder, add 32.5 times the mass of water, boil and cool to room temperature to obtain product 1;

[0103] S2: adding 600u / g of cellulase to product one, performing the first enzymolysis at 50°C and 60rpm for 3.5 hours, and then inactivating the enzyme; then adding 500U / g of ginger protease to product one, performing the second enzymolysis at 52°C and 70rpm for 3.7 hours, and then inactivating the enzyme to obtain product two;

[0104] S3: Add 2.2% of glucose and 0.0075% of potassium dihydrogen phosphate, a nitrogen source, to product 2, mix well, then add fermentation bacteria, stir at 35°C and 40rpm for anaerobic fermentation for 185 hours, sterilize, and obtain product 3; wherein, the amount of fermentation bacteria is 6.80×10 9 cfu / g product 2; the fermentation bacteria consisted of Bacillus licheniformis CICC 10103 and Bacillus plantarum CICC 21834 in a quantitative ratio of 1:1;

[0105] S4: Add ethanol to the product three to adjust the ethanol mass concentration to 60%, heat at 50°C, stir at 80rpm for 6 hours, filter, and obtain a filtrate, which is the product four;

[0106] S5: The product 4 is concentrated under reduced pressure at 55° C. and 0.006 MPa to 10.2% of the mass of the product 4, and then freeze-dried at -45° C. to a water content of 2.76 wt %, to obtain the camellia seed extract.

[0107] 2. Preparation of cosmetic preparations

[0108] Rosemary extract: Shaanxi Pinhong Biotechnology Co., Ltd. Green tea extract: Shaanxi Huinengda Biotechnology Co., Ltd. Witch hazel extract: Shanyang Lianfeng Biotechnology Co., Ltd. Alkyl glycoside: APG0810.

[0109] Preparation A:

[0110] The raw material composition is as follows: mass percentage measurement: 4% camellia seed extract, 0.5% rosemary extract, 0.8% green tea extract, 0.4% witch hazel extract, 2.5% soapberry saponin, 4.8% alkyl glycoside, 1.2% carbomer-940, 0.3% carrageenan, 7% glycerol, 0.3% sodium citrate, 0.2% phenoxyethanol and the balance water.

[0111] The preparation method is as follows: heat water to 60°C, add soapberry saponin, alkyl glycoside, carbomer-940, carrageenan, and glycerin, stir at 100 rpm for 20 minutes, then add the remaining ingredients, continue stirring for 30 minutes, let stand for aging for 0.5 hours, sterilize, and then fill to obtain the preparation.

[0112] Preparation B:

[0113] The raw material composition is as follows: mass percentage measurement: 4.05% camellia seed extract II, 0.7% rosemary extract, 0.5% green tea extract, 0.6% witch hazel extract, 2.3% soapberry saponin, 5.0% alkyl glycoside, 1.1% carbomer-940, 0.45% carrageenan, 7% glycerol, 0.35% sodium citrate, 0.22% phenoxyethanol and the balance water.

[0114] The preparation method is as follows: heat water to 62° C., add soapberry saponin, alkyl glycoside, carbomer-940, carrageenan, and glycerin, stir at 120 rpm for 15 minutes, then add the remaining ingredients, continue stirring for 30 minutes, let stand for aging for 0.6 hours, sterilize, and then fill to obtain the preparation.

[0115] The difference between the preparations CI and the preparation A is that the camellia seed extracts 3 to 9 are used to replace the camellia seed extract 1 in the preparation A. The other parts are the same.

[0116] (II) Performance test

[0117] 1. Security testing

[0118] 90 healthy volunteers aged 30-45 years were selected and randomly divided into 9 groups with 10 people in each group (5 males and 5 females). The safety of the above-prepared preparations was tested according to the skin occlusion patch test method recorded in the "Technical Specifications for Safety of Cosmetics". The test results were evaluated according to the skin reaction grading standards recorded in Table 1. The test results are shown in Table 2.

[0119] Table 1: Skin occlusion patch test skin reaction grading criteria

[0120]

[0121] Table 2: Test results

[0122] Test / Number of people Level 0 Level 1 Level 2 Level 3 Level 4 Preparation A 10 0 0 0 0 Preparation B 10 0 0 0 0 Preparation C 10 0 0 0 0 Preparation D 10 0 0 0 0 Preparation E 10 0 0 0 0 Formulation F 10 0 0 0 0 Formulation G 10 0 0 0 0 Preparation H 10 0 0 0 0 Formulation I 10 0 0 0 0

[0123] 2. Decontamination and oil control test

[0124] 45 male volunteers aged 30-45 with healthy skin were selected and randomly divided into 9 groups (5 volunteers in each group) according to age. Each group of volunteers used the above preparation to wash their faces once every night (rubbing for three minutes and rinsing with water) for 2 consecutive months. Before using the above preparation and after 2 months of use, the skin multifunctional test system SM815 was used to test the oil content (μg / cm 2), test conditions: humidity 50±2%, temperature 25±1℃. Among them, the test pretreatment conditions before using the preparation were: washing the face with clean water and wiping it with a paper towel before testing; the pretreatment conditions for the test 2 months after using the preparation were: washing the face with clean water and wiping it with a paper towel 2 hours after the last face washing before testing. At the same time, the reduction rate of oil content on the cheeks of each group of volunteers was calculated. The test results are shown in Table 3.

[0125] Table 3: Decontamination and Oil Control Test

[0126]

[0127] 3. Moisturizing test

[0128] When conducting the above-mentioned decontamination and oil control test experiment, the water content of the stratum corneum of the cheek skin was tested using the instrument CM825 before and after using the above-mentioned preparation. The test conditions were: humidity 50±2%, temperature 25±1°C. At the same time, the increase rate of the water content of the stratum corneum of the cheek skin of each group of volunteers was calculated. The test results are shown in Table 4.

[0129] Table 4: Moisture test

[0130]

[0131] Combined with the test results in Tables 2-4, it can be seen that the cosmetics prepared by the present invention are low in irritation, highly safe, and have good effects of removing dirt, controlling oil, and increasing skin moisture content.

[0132] According to the test results of preparations A and BI in Tables 3-4, after the extraction method of camellia seed extract is changed, the obtained preparation effect is worse than the cleansing, oil-controlling and moisturizing effects of preparation A. It can be seen that the camellia seed extract obtained by the specific preparation method of the present invention has excellent cleansing, oil-controlling and skin moisturizing effects.

[0133] According to the test results of preparations A and C, the camellia seed extract prepared by enzymatic hydrolysis with ginger protease in the present invention has better cleansing, oil control and moisturizing effects.

[0134] According to the test results of preparations A and DE, the present invention adopts Bacillus licheniformis CI CC 10103 and Bacillus plantarum CI CC 21834 to jointly ferment to prepare camellia seed extract, which has better effect than using only one of them; and when the number of fermentation bacteria is kept unchanged, the oil control and moisturizing properties obtained by fermentation of the two are better; thus, it can be seen that when the two are jointly fermented, a synergistic relationship is generated.

[0135] According to the test results of preparations AB and GH, the fermentation bacteria of the present invention are composed of Bacillus licheniformis CI CC 10103 and Bacillus plantarum CI CC 21834 in a quantity ratio of 1:0.8-1.3 for fermentation. Compared with fermentation with other proportions of bacteria combinations, the camellia seed extract obtained has better effect.

[0136] According to the test results of preparations A and I, the present invention uses ethanol aqueous solution to further extract the fermented product, which can increase the cleansing, oil control and moisturizing effects of the extract, and when the concentration of ethanol aqueous solution is 15-35wt%, the extraction effect is best.

[0137] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A method for preparing a camellia seed extract having oil control and decontamination functions, characterized in that: The following steps are involved: S1: crushing camellia seeds to obtain camellia seed powder, adding water to boil, cooling, and obtaining product 1; S2: adding cellulase to product 1, performing enzymolysis for the first time, and then killing the enzyme; then adding ginger protease, performing enzymolysis for the second time, and then killing the enzyme, to obtain product 2; S3: adding glucose and nitrogen source to product 2, mixing, then adding fermentation bacteria, fermenting, sterilizing, and obtaining product 3; S4: adding ethanol to the product three, adjusting the ethanol concentration, heating and stirring, filtering, and obtaining a filtrate, which is the product four; S5: The product 4 is concentrated under reduced pressure and dried to obtain the camellia seed extract.

2. The preparation method according to claim 1, characterized in that: In S1, the mass of water added is 10-40 times that of camellia seed powder.

3. The preparation method according to claim 1, characterized in that: In S1, the amount of cellulase added is 500-800u / g product 1, and the temperature and time of the first enzymatic hydrolysis are 40-60°C and 2-5 hours.

4. The preparation method according to claim 1, characterized in that: In S2, the amount of ginger protease added is 400-700 U / g product 1, and the temperature and time of the second enzymatic hydrolysis are 40-60° C. and 2-5 hours.

5. The preparation method according to claim 1, characterized in that: In S3, the added amounts of glucose and nitrogen source are 1-3% and 0.002-0.01% of the mass of product II, respectively; The fermentation temperature is 30-40°C and the fermentation time is 120-200 hours.

6. The preparation method according to claim 1, characterized in that: In S3, the fermentation bacteria dosage is 3.5×10 9 -8.5×10 9 cfu / g product 2; the fermentation bacteria consisted of Bacillus licheniformis CICC 10103 and Bacillus plantarum CICC 21834 in a quantity ratio of 1:0.8-1.

3.

7. The preparation method according to claim 1, characterized in that: In S4, the ethanol mass concentration is adjusted to 15-35%, and the heating and stirring temperature and time are 40-60° C. and 4-10 hours.

8. A camellia seed extract with oil-controlling and decontamination-removing functions prepared by the preparation method according to any one of claims 1 to 7.

9. A use of the camellia seed extract having oil control and decontamination function in the preparation of cosmetics according to claim 8, characterized in that: The mass percentage of the camellia seed extract with oil-controlling and dirt-removing functions in cosmetics is 0.5-10%.

10. The use according to claim 9, characterized in that: The cosmetics further include, by mass percentage, 0.1-2% rosemary extract, 0.1-2% green tea extract, 0.2-2% witch hazel extract, 1-5% soapberry saponin, 1-5% alkyl glycoside, 1-3% thickener, 3-8% polyol, 0.1-0.5% sodium citric acid, 0.08-0.3% preservative and the balance water.

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