Tight junction protein production promoter

Abstract

The present invention relates to a novel tight junction protein (Claudin-1) production promoter, which is characterized by comprising only a polygonatum odoratum extract, a skin barrier repair agent containing the tight junction protein (Claudin-1) production promoter, and a cosmetic, and more particularly, to a novel tight junction protein (Claudin-1) production promoter, which is characterized by comprising only a polygonatum odoratum extract, a skin barrier repair agent containing the tight junction protein (Claudin-1) production promoter, and a cosmetic containing the tight junction protein (Claudin-1) production promoter.

Description

Technical Field

[0001] The present invention relates to a novel promoter for generating claudin-1, which is characterized by containing only polygonatum odoratum extract, a skin barrier repair agent containing the promoter for generating claudin-1, and a cosmetic product. Background Art

[0002] Generally speaking, the skin barrier includes many aspects such as physical barrier, pigment barrier, nerve barrier and immune barrier. Among them, the physical barrier structure is composed of the stratum corneum (SC) and tight junctions (TJ), and both jointly prevent the penetration of external antigens from outside to inside or the leakage of internal components from inside to outside. Compared with the stratum corneum, tight junctions are a highly dynamic structure, which play a role in closing intercellular spaces, controlling substance transport, maintaining cell polarity, etc., and regulating the permeability of epidermal water and solutes.

[0003] Tight junctions are composed of the claudin family of proteins. Claudins are a large family of membrane proteins, first discovered in chicken liver. Currently, 27 members of the claudin family have been discovered in mammals, and they are roughly named Claudin-1 to Claudin-27 in the order of discovery, and are encoded by genes CLDN1 to CLDN27 respectively. Their functions mainly include participating in regulating extracellular permeability, cell polarity, differentiation and proliferation, migration and apoptosis, and mediating cell signal transduction. As an important member of the claudin family, claudin-1 is one of the main constituent proteins of tight junctions.

[0004] Claudins have many functions, and their main roles include the following aspects: ① Claudins can tightly connect epithelial cells, making the cells not easily damaged, so they are an important part of the skin barrier function; ② Tight junction structures and / or claudin proteins can control the paracellular pathway of molecules, and have selectivity for molecular size, ion type, and cell permeability (barrier function); ③ They can separate the lipids at the basal part of the cell membrane to the apical part of the cell membrane to form two different membrane functional regions, thereby preventing the mutual diffusion between the two different functional regions, maintaining the proprietary functions of each surface of the cell, and maintaining cell polarity (separation effect). Tight junction structures / proteins also participate in signal transduction pathways and cell surface receptors such as transforming growth factor-β receptors, and participate in cell proliferation and differentiation as well as vesicle transport processes.

[0005] Therefore, promoting the production of tight junction proteins can help enhance the skin barrier function, maintain cell function, promote cell proliferation and differentiation, metabolism, maintain skin structure and elasticity, improve skin problems such as sensitivity, fine lines, and texture, and is of great significance for skin stability and anti-aging.

[0006] In the prior art, in WO 2016 / 067688 (published on May 6, 2016), a skin barrier function improver containing an inositol derivative formed by combining inositol and sugar as an active ingredient was disclosed, and it was disclosed that this skin barrier function improver can promote the production of tight junction proteins. In CN115836999A (published on March 24, 2023), an epidermal protein promoter and its preparation method and application were disclosed. Enzymes were added to rice milk and enzymatically hydrolyzed to obtain a rice milk enzymatic hydrolysate; yeast was inoculated into the rice milk enzymatic hydrolysate and fermented to obtain a fermentation broth; the fermentation broth containing active substances was collected, which is the epidermal protein promoter, and the active substance is cytidine. The epidermal protein promoter provided by this invention can be used as an expression promoter for epidermal tight junction proteins claudin-1 and claudin-4, an expression promoter for occludin, an expression promoter for aquaporin mRNA, and an expression promoter for hyaluronic acid synthase 3 mRNA in fibroblasts; it can be used as an epidermal protein promoter in products.

[0007] Prior art documents

[0008] Patent documents

[0009] 1. WO 2016 / 067688

[0010] 2. CN115836999A

[0011] Based on such a cognition, the applicant has made efforts to find new tight junction protein production promoters for the development of new skin barrier repair agents and cosmetics with skin barrier repair effects. Summary of the invention

[0012] Technical problems to be solved by the invention

[0013] The object of the present invention is to find new tight junction protein production promoters with the function of promoting the production of tight junction proteins, and then develop skin barrier repair agents containing new tight junction protein production promoters and cosmetics with skin barrier repair functions.

[0014] Means for solving the technical problems

[0015] In order to find a new promoter for the production of tight junction proteins, the present inventor conducted a large number of experiments. As a result, it was found that polygonatum odoratum extract has the effect of promoting the production of tight junction protein (Claudin-1). The polygonatum odoratum extract can act as a promoter for the production of tight junction proteins and can therefore be used as a skin barrier repair agent. Furthermore, the present inventor found that the polygonatum odoratum extract can be used as a promoter for the production of tight junction proteins to promote the production of tight junction proteins in the skin, and thus promote the repair of the skin barrier. Therefore, the polygonatum odoratum extract can be applied to cosmetics that require skin barrier repair function, and thus the present invention was completed.

[0016] The present invention provides the following solutions:

[0017] Solution 1. A promoter for the production of tight junction protein (Claudin-1), characterized in that it consists only of polygonatum odoratum extract.

[0018] Solution 2. A skin barrier repair agent, characterized in that it contains polygonatum odoratum extract.

[0019] Solution 3. Use of polygonatum odoratum extract as a promoter for the production of tight junction protein (Claudin-1).

[0020] Solution 4. Use of polygonatum odoratum extract for the preparation of a promoter for the production of tight junction protein (Claudin-1).

[0021] Solution 5. Use of polygonatum odoratum extract in the preparation of cosmetics for promoting the production of tight junction proteins.

[0022] Solution 6. Use of polygonatum odoratum extract in the preparation of cosmetics for promoting skin barrier repair.

[0023] Effects of the Invention

[0024] The present invention for the first time discovers that polygonatum odoratum extract can promote the production of tight junction proteins and can be used as a promoter for the production of tight junction proteins. Furthermore, the present invention can provide a new promoter for the production of tight junction proteins containing polygonatum odoratum extract as an active ingredient, and a skin barrier repair agent containing polygonatum odoratum extract as a promoter for the production of tight junction proteins. Description of the Drawings

[0025] Figure 1 It is a figure showing the promoting effect of polygonatum odoratum extract on the expression of tight junction protein CLDN-1 in skin cells Detailed Description of the Invention

[0026] The present invention is described in detail below, but the present invention is not limited to the specific embodiments listed below, and the present invention can be implemented with various modifications within the scope not departing from the gist of the present invention.

[0027] The "polygonatum odoratum extract" in the present invention is extracted from the dried rhizome of Polygonatum odoratum (Mill.) Druce of the Liliaceae family, and contains components such as polygonatum polysaccharide, steroidal saponin, and homoisoflavanone. Polygonatum odoratum is the dried rhizome of Polygonatum odoratum (Mill.) Druce of the Liliaceae family. It is also known as tail ginseng and jade ginseng. It grows under forests or between rocks in the mountains and likes shady and humid places. It is distributed in most parts of my country. It was first recorded in the "Shennong Bencao Jing" under the name of wilt, and was listed as a top grade. It is flat in nature, sweet in taste, and has the functions of nourishing yin and moistening dryness, promoting body fluid and quenching thirst. It is used for lung and stomach yin damage, dry heat cough, dry throat and thirst, and internal heat and thirst. Modern pharmacological research has shown that Polygonatum odoratum has the main effects of strengthening the heart, lowering blood pressure, lowering blood sugar, lowering blood lipids and enhancing immune function. It is very beneficial for patients with diabetes and hypertension and hyperlipidemia. It is one of the 87 plants that can be used as both medicine and food announced by the Ministry of Health.

[0028] Polygonatum odoratum extract contains a variety of ingredients, the main chemical components are as follows:

[0029] 1. Polysaccharide ingredients

[0030] The content of the main active ingredient of Polygonatum odoratum is 6.51% to 10.27%. The composition of Polygonatum odoratum mucopolysaccharide is D-fructose, D-mannose, D-glucose and D-galacturonic acid, with a molar ratio of 6:3:1:1.5. In addition, it also contains Polygonatum odoratum fructan A, B, C and D, which are composed of glucose and fructose, with a composition ratio of 97.5:2.6, 96.2:3.8, 95.3:4.8 and 90.4:9.6.

[0031] 2. Steroidal saponins

[0032] Steroidal saponin components are also the active ingredients of Polygonatum odoratum, with a content of 0.2186% - 0.3572%. The steroidal saponin components in Polygonatum odoratum include convallamarin, convallarin, 25(R,S)-spirost-5-ene-3β,14α-diol-3-O-β-D-glucopyranosyl(1→2)-[β-D-glucopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside, 25(R,S)-spirost-5-ene-3β-ol-3-O-β-D-glucopyranosyl(1→2)-[β-D-glucopyranosyl(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside. In addition, it also contains polygonatum C, POD, polygonatospirostanol POa, polygonatospirostanoside POb, POe, PO1, PO2, PO3, PO4, PO5, PO6, PO7, PO8 and PO9 and other steroid compounds, as well as 22-hydroxy-25(R,S)-furost-5-ene-12-one-3β,22,26-triol-26-O-β-D-glucopyranoside, etc.

[0033] 3. Flavonoid components

[0034] Most of the flavonoid components in Polygonatum odoratum are homo-isoflavanones. The known flavonoid components include chelidonic acid, azetidine-2-carboxylic acid, kaempferol arabinoside, polygonatin, vitexin-2''-O-sophoroside and vitexin, vitexin-2''-O-glucoside, etc.

[0035] 4. Volatile oil components

[0036] Using the GC-MS method, 40 components were detected in the volatile oil of Polygonatum odoratum, and 32 compounds were identified. The main components are unsaturated olefins, accounting for 37.05%, followed by some alcohols, aldehydes, acids, esters and alkanes.

[0037] 5. Nutritional components

[0038] Polygonatum odoratum is rich in nutrients, containing a variety of amino acids. The total amino acid content is 11.22% - 12.20%, and the free amino acid content is 16.87 - 220.23 μmol·g-1. Among them, the essential amino acid content is 3.54% - 3.87%, and the semi-essential amino acid content is 1.25% - 1.70%; Polygonatum odoratum also contains a variety of trace elements, such as Ca, P, Mg, Cu, Zn, Fe, Mn, Cd, Na, etc. Among them, the contents of Ca, Mg, P, Cu, etc. are relatively rich, and these trace elements play an important role in human metabolism.

[0039] POD-Ⅰ: Chemical name: 25(R,S)spirost-5-ene-3β-ol-3-O-β-D-glucopyranoside-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside. Molecular formula C 50 H 80 O 22 , molecular weight 1032. White granular crystals, mp. 252 °C - 255 °C (dec.), [α]D17 - 78.2 °C (C = 0.21, C5H5N).

[0040] POD-Ⅱ: Chemical name: 25(R)spirost-5-ene-3β,14α-diol-3-O-β-D-glucopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside. Molecular formula C 50 H 80 O 23 , molecular weight 1048. White fine needle-like crystals, mp. 257 °C - 260 °C (dec.), [α]D17 - 81.3° (C = 0.17, C5H5N). Positive Liebermann-Burchard reaction, does not show red with Ehrlich reagent.

[0041] POD-Ⅲ: Chemical name: 25(R,S)spirost-5-ene-3β,14α-diol-3-O-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl(1→4)-β-D-galactopyranoside. Molecular formula C 51 H 82 O 24 , molecular weight 1078. White fine needle-like crystals, mp. 243 °C - 245 °C (dec), [α]D17 - 72.5° (C = 0.42, C5H5N).

[0042] POD-Ⅳ: Chemical name: 25(R,S)spirost-5-ene-3β-ol-3-O-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside. Molecular formula C51H82O23, molecular weight 1062. White needle-like crystals, mp. 279 °C - 281 °C (dec), [α]D17 - 68° (C = 0.39, C5H5N).

[0043] The pharmacological effects of Polygonatum odoratum known in the prior art include:

[0044] 1. Hypoglycemic effect: Polygonatum odoratum polysaccharide has hypoglycemic and antioxidant stress effects, and has an obvious protective effect on the damage of pancreatic islet B cells in diabetic rats induced by alloxan. It can dose-dependently improve the weight loss of diabetic rats, reduce fasting blood glucose and the content of MDA in pancreatic tissue, and enhance the activities of SOD, GSH-Px, and CAT. Polygonatum odoratum extract A can reduce the blood glucose of type I diabetic mice by restoring the balance of CD4+ and CD8+ T lymphocyte subsets and regulating the levels of cytokines during the disease process. The total extract of Polygonatum odoratum and its chloroform-separated part both have obvious hypoglycemic effects and obvious protective effects on kidney lesions, and the chloroform-separated part has a longer maintenance time. Total saponins of Polygonatum odoratum can reduce the blood glucose of alloxan-induced hyperglycemic mice by inhibiting the activity of α-glucosidase.

[0045] 2. Antioxidant effect: The monomeric compounds feruloyltyramide and homoisoflavone in Polygonatum odoratum have obvious protective effects on the oxygen free radical damage of PC12 cells, and the cell survival rate increases significantly. The water extract of Polygonatum odoratum can, to a certain extent, reduce the damage of medium-wave ultraviolet rays to keratinocytes and inhibit the decrease in fibroblast activity caused by long-wave ultraviolet rays. The mechanism may be to inhibit oxidative damage, enhance the antioxidant ability of cells, and reduce the secretion of inflammatory cytokines. The crude extract of Polygonatum odoratum glycoprotein has certain antioxidant activity, which can increase the activities of SOD, CAT, and GSH-Px in the serum, liver, and brain of mice and reduce the content of MDA.

[0046] 3. Immunomodulatory effect: The extract of Polygonatum odoratum can enhance immune function, increase the weights of the thymus and spleen, the phagocytosis percentage and phagocytosis index of macrophages in immunosuppressed model mice induced by cyclophosphamide, promote the formation of hemolysin and hemolytic plaque, and increase the lymphocyte transformation rate. The extract of Polygonatum odoratum can significantly inhibit the transformation and proliferation of T lymphocytes and reduce the release of liver injury cytokines in a dose-dependent manner, thereby playing a liver protection role.

[0047] 4. Antitumor effect: The extract of Polygonatum odoratum has a significant antitumor effect. It can inhibit the proliferation of human esophageal cancer cell Eca-109 and induce its apoptosis. It can enhance cellular immune function by promoting the secretion of IL-2 by tumor-bearing mouse cells and the secretion of IL-1 and TNF-α by peritoneal macrophages, and directly induce the apoptosis of tumor cells to promote the apoptosis of human colon cancer cell CL-187.

[0048] 5. Anti-aging effect: Polygonatum odoratum polysaccharide can increase the activity of serum superoxide dismutase in aging mice, enhance the ability to scavenge free radicals, inhibit lipid peroxidation, and reduce the content of MDA. At the same time, it has a certain regulatory effect on the immune function of sub-acutely aging mice, can improve the immune imbalance state, and enhance cellular and humoral immune functions, thus delaying aging. The water extract of Polygonatum odoratum can reduce the oxidative damage of the sub-acute aging mouse model induced by D-galactose, protect the mitochondrial DNA of hepatocytes, and delay the aging of the body.

[0049] 6. Effects on the cardiovascular system: The decoction of Polygonatum odoratum has a positive inotropic effect on isolated frog hearts and isolated rat hearts. Total saponins of Polygonatum odoratum can significantly improve the myocardial contractility and relaxation function of rats. The serum of rats containing 4% Polygonatum odoratum can slow down the heart rate of neonatal rat cardiomyocytes, indicating that Polygonatum odoratum has a certain effect on improving myocardial function; the sera of rats containing 1%, 2%, and 4% Polygonatum odoratum can significantly reduce the content of lactate dehydrogenase and reduce the damage caused by hypoxia and glucose deficiency to cardiomyocytes.

[0050] 7. Other effects: The 80% ethanol extract of Polygonatum odoratum has the effect of activating tyrosinase, and its activation rate of tyrosinase reaches 29% at the level of 5 mg (equivalent to the original drug amount of 10 mg·mL-1); the kinetic observation results on whether Polygonatum odoratum can improve the activity of tyrosinase show that Polygonatum odoratum can promote the binding of tyrosinase to the substrate and thus accelerate the reaction rate. Therefore, Polygonatum odoratum can be applied to the treatment of pigment deficiency diseases and catecholamine neurotransmitter synthesis disorder diseases caused by the decrease or reduction of tyrosinase activity in clinical practice; its activation effect on tyrosinase suggests that Polygonatum odoratum may be a potential drug for the treatment of vitiligo. The extract of Polygonatum odoratum (EC-PAOA, the alcohol-water extract of Polygonatum odoratum, is a polysaccharide component) can significantly inhibit the proliferation of eutopic endometrial cells in patients with endometriosis. Its in vitro experimental study shows that EC-PAOA can reduce the expression of the multifunctional cytokine L-6 and the endometrial epithelial cell-specific protein CA-125, which are most closely related to endometriosis, so as to reduce the formation of new blood vessels at the lesion site, accelerate the necrosis of ectopic lesions, promote the absorption of local inflammation, and reduce pelvic adhesions; at the same time, it reduces the local estrogen content in ectopic lesions, hinders the growth of ectopic cells, effectively controls the development of endometriosis, further improves the intraperitoneal environment, and makes the cell components in peritoneal fluid transform into normal.

[0051] The inventor of the present invention first discovered that the extract of Polygonatum odoratum has the effect of promoting the generation of tight junction proteins and proved this effect through experiments.

[0052] The preparation method of the extract of Polygonatum odoratum in the present invention is as follows: The powder of Polygonatum odoratum is ultrasonically stirred with an alcohol-water mixture and then heated for extraction. After drying and concentrating the product, the product is dissolved in a stable system for easy storage.

[0053] The alcohol-water mixture can use a mixture of alcohol with 1 to 6 carbon atoms and water. As the alcohol, methanol, ethanol, 1-propanol, 2-propanol, n-butanol, sec-butanol, tert-butanol, n-pentanol, isopentanol, hexanol, glycerol, butanediol, pentaerythritol, etc. can be listed. Ethanol is particularly preferably used.

[0054] As the alcohol-water mixing ratio of the alcohol-water mixture, the mass ratio of alcohol to water is preferably 5:1 to 1:5, more preferably 4:1 to 1:4, further preferably 3:1 to 1:3, and most preferably 1:1.

[0055] There is no particular limitation on the temperature for heating extraction, and it can be arbitrarily selected within the range of 10 to 95 °C, preferably 30 to 90 °C.

[0056] The heating extraction time is preferably 4 to 10 hours, more preferably 5 to 9 hours, and further preferably 6 to 8 hours.

[0057] The dried and concentrated product is preferably dissolved in a stable system. The stable system is preferably an alcohol-water solvent system, and the alcohol-water solvent system can use a mixture of an alcohol having 1 to 6 carbon atoms and water. As the alcohol, methanol, ethanol, 1-propanol, 2-propanol, n-butanol, sec-butanol, tert-butanol, n-pentanol, isopentanol, hexanol, glycerol, butanediol, pentaerythritol, etc. can be cited. Particularly preferably, butanediol is used.

[0058] As the alcohol-water mixing ratio of the alcohol-water mixture, the mass ratio of alcohol to water is preferably 10:1 to 1:10, more preferably 9:1 to 1:9.

[0059] After the polygonatum odoratum extract is dissolved in the stable system, decolorization and deodorization treatment can be carried out according to the conventional techniques in the art. For example, activated carbon can be used for decolorization and deodorization treatment.

[0060] After the decolorization and deodorization treatment, low-temperature standing can be carried out. The temperature range for the low-temperature standing is preferably 0 to 10 °C, more preferably 6 to 8 °C.

[0061] The time for low-temperature standing can be 36 to 72 hours, more preferably 48 hours.

[0062] After low-temperature standing, filtration is carried out to remove insoluble matters by filtration.

[0063] After that, a preservative system is added to obtain the polygonatum odoratum extract.

[0064] As the preservative system, preservatives and preservative aids such as phenoxyethanol, benzyl alcohol, methylparaben, hexylene glycol, butanediol, p-hydroxyacetophenone, etc. can be cited, for example.

[0065] The obtained polygonatum odoratum extract is sent to the quality control department for testing. The quality inspection standards are as follows: visually, the color is a yellow transparent liquid with a characteristic odor; pH = 4 to 7, the polysaccharide content is greater than 0.2%, and the microbial and heavy metal indicators meet the requirements of the "Technical Specifications for Cosmetics Safety".

[0066] In addition, the method for obtaining the polygonatum odoratum extract in the present invention is not particularly limited, and it can be obtained by separating from polygonatum odoratum plants or by using known methods for extraction or using commercially available products.

[0067] The "skin barrier repair agent" described in the present invention refers to a topical skin agent having the function of repairing the skin barrier, and refers to any preparation externally applied to the skin, including but not limited to cosmetics. For example, aqueous solution preparations such as toner, lotion, moisturizing lotion, repair lotion, etc.; spray preparations such as moisturizing spray, anti-wrinkle spray, repair spray, etc.; lotion preparations such as moisturizing lotion, anti-wrinkle lotion, repair lotion, body lotion, hand lotion, etc.; essence preparations such as moisturizing essence, anti-wrinkle essence, repair essence, etc.; gel preparations such as moisturizing gel, anti-wrinkle gel, repair gel, etc.; cream or cream preparations such as face cream, eye cream, moisturizing cream, anti-wrinkle cream, repair cream, massage cream, hand cream, body cream, etc.; powder preparations such as setting powder, loose powder, powder cake, talcum powder, etc.

[0068] The new skin barrier repair agent of the present invention is characterized in that it contains polygonatum extract as an active ingredient for promoting the generation of tight junction proteins.

[0069] The content of the polygonatum extract in the skin barrier repair agent is 0.0001% by mass to 5% by mass, more preferably 0.001% by mass to 2% by mass, further preferably 0.01% by mass to 1.5% by mass, and particularly preferably 0.1% by mass to 1% by mass.

[0070] In addition to containing polygonatum extract as an active ingredient of the skin barrier repair agent, the topical skin agent of the present invention may contain other additives such as excipients and carriers, and components usually added in cosmetics can be appropriately used as needed.

[0071] Components usually added in cosmetics include but are not limited to, for example, aqueous solvents, oily components, anionic surfactants, cationic surfactants, amphoteric surfactants, nonionic surfactants, water-soluble polymers, thickeners, preservatives, sequestering agents, amino acids, organic amines, polymer emulsions, pH regulators, neutralizing agents, skin nutrients, vitamins, antioxidants, antioxidant aids, fragrances, etc.

[0072] Examples of the aqueous solvent include water, alcohol, humectant or a mixture thereof.

[0073] As water, water used in cosmetics, quasi-drugs, etc. can be used. For example, pure water, ion-exchanged water, tap water, etc. can be used. Depending on the purpose, the aqueous phase may further contain water-soluble alcohol.

[0074] Examples of the water-soluble alcohol include at least one selected from the following: lower alcohols, polyhydric alcohols, polyhydric alcohol polymers, glycol alkyl ethers, glycol ether esters, glycerol monoalkyl ethers, sugar alcohols, monosaccharides, oligosaccharides, polysaccharides and their derivatives, etc.

[0075] Examples of the lower alcohol include ethanol, propanol, isopropanol, isobutanol, tert-butanol, etc.

[0076] Examples of polyols include: diols (e.g., ethylene glycol, propylene glycol, trimethylene glycol, 1,2 - butanediol, 1,3 - butanediol, tetramethylene glycol, 2,3 - butanediol, pentamethylene glycol, 2 - butene - 1,4 - diol, hexanediol, octanediol, etc.); triols (e.g., glycerol, trimethylolpropane, etc.); tetrols (e.g., pentaerythritol, etc.); pentols (e.g., xylitol, etc.); hexols (e.g., sorbitol, mannitol, etc.).

[0077] Examples of polyol polymers include, for example, diethylene glycol, dipropylene glycol, triethylene glycol, polypropylene glycol, tetraethylene glycol, diglycerol, triglycerol, tetraglycerol, polyethylene glycol, etc.

[0078] Examples of dialkyl ethers of diols include, for example, ethylene glycol monomethyl ether, ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, ethylene glycol monophenyl ether, ethylene glycol monohexyl ether, ethylene glycol mono - 2 - methylhexyl ether, ethylene glycol isoamyl ether, ethylene glycol benzyl ether, ethylene glycol isopropyl ether, ethylene glycol dimethyl ether, ethylene glycol diethyl ether, ethylene glycol dibutyl ether, etc.; diethylene glycol monomethyl ether, diethylene glycol monoethyl ether, diethylene glycol monobutyl ether, diethylene glycol dimethyl ether, diethylene glycol diethyl ether, diethylene glycol butyl ether, diethylene glycol methyl ethyl ether, triethylene glycol monomethyl ether, triethylene glycol monoethyl ether, propylene glycol monomethyl ether, propylene glycol monoethyl ether, propylene glycol monobutyl ether, propylene glycol isopropyl ether, dipropylene glycol methyl ether, dipropylene glycol ethyl ether, dipropylene glycol butyl ether, etc.

[0079] Examples of diol ether esters include, for example, ethylene glycol monomethyl ether acetate, ethylene glycol monoethyl ether acetate, ethylene glycol monobutyl ether acetate, ethylene glycol monophenyl ether acetate, ethylene glycol diadipate, ethylene glycol disuccinate, diethylene glycol monoethyl ether acetate, diethylene glycol monobutyl ether acetate, propylene glycol monomethyl ether acetate, propylene glycol monoethyl ether acetate, propylene glycol monopropyl ether acetate, propylene glycol monophenyl ether acetate, etc.

[0080] Examples of glycerol monoalkyl ethers include, for example, batyl alcohol, selachyl alcohol, chimyl alcohol, etc.

[0081] Examples of sugar alcohols include, for example, sorbitol, maltitol, maltotriose, mannitol, sucrose, erythritol, glucose, fructose, starch - degrading sugar, maltose, xylose, starch - degrading sugar reduction alcohol, etc.; tetrahydrofurfuryl alcohol; POE - tetrahydrofurfuryl alcohol; POP - butyl ether; POP·POE - butyl ether; polyoxypropylene glycerol ether; POP - glycerol ether; POP - glycerol ether phosphate; POP·POE - pentaerythritol ether, etc.

[0082] As monosaccharides, for example, at least one selected from the following can be cited: trioses (e.g., D-glyceraldehyde, dihydroxyacetone, etc.), tetroses (e.g., D-erythrose, D-erythrulose, D-threose, erythritol, etc.), pentoses (e.g., L-arabinose, D-xylose, L-lyxose, D-arabinose, D-ribose, D-ribulose, D-xylulose, L-xylulose, etc.), hexoses (e.g., D-glucose, D-talose, D-allulose, D-galactose, D-fructose, L-galactose, L-mannose, D-tagatose, etc.), heptoses (e.g., heptaldose, heptulose, etc.), octoses (e.g., octulose, etc.), deoxysugars (e.g., 2-deoxy-D-ribose, 6-deoxy-L-galactose, 6-deoxy-L-mannose, etc.), amino sugars (e.g., D-glucosamine, D-galactosamine, sialic acid, muramic acid, etc.), uronic acids (e.g., D-glucuronic acid, L-guluronic acid, D-galacturonic acid, L-iduronic acid, etc.), etc.

[0083] As oligosaccharides, for example, at least one selected from the following can be cited: sucrose, umbelliferone sugar, lactose, plantain sugar, α,α-trehalose, etc.

[0084] As polysaccharides, for example, at least one selected from the following can be cited: cellulose, quince seeds, chondroitin sulfate, erythritol, starch, galactan, dermatan sulfate, glycogen, gum arabic, heparan sulfate, hyaluronic acid, tragacanth gum, keratan sulfate, chondroitin, xanthan gum, mucin sulfate, guar gum, dextran, locust bean gum, succinoglycan, calonic acid, etc.

[0085] As other polyhydric alcohols, for example, at least one selected from polyoxyethylene methyl glucoside (Glucam E-10), polyoxypropylene methyl glucoside (Glucam P-10), etc. can be cited.

[0086] As humectants, for example, the following can be cited: propylene glycol, glycerin, 1,3-butanediol, dipropylene glycol, xylitol, sorbitol, maltitol, chondroitin sulfate, hyaluronic acid, mucin sulfate, calonic acid, telopeptide-free collagen, sodium lactate, bile salts, dl-pyrrolidone carboxylate, alkylene oxide derivatives, short-chain soluble collagen, diglycerol (EO)PO adduct, rose hip extract, yarrow extract, melilot extract, etc.

[0087] As oily components, for example, liquid oils, solid fats, waxes, hydrocarbons, higher fatty acids, higher alcohols, synthetic ester oils, silicone oils, etc. can be used. It should be noted that in this specification, oil components and components soluble in oil components are also included and referred to as "oily components".

[0088] As liquid oils and fats, for example, the following can be cited: avocado oil, camellia oil, turtle oil, macadamia nut oil, corn oil, mink oil, olive oil, rapeseed oil, egg yolk oil, sesame oil, peach kernel oil (almond oil), wheat germ oil, camellia flower oil, castor oil, linseed oil, safflower oil, cottonseed oil, perilla oil, soybean oil, peanut oil, tea seed oil, torreya nucifera oil, rice bran oil, Chinese tung oil, Japanese tung oil, jojoba oil, germ oil, triglyceride, etc.

[0089] As solid oils and fats, for example, the following can be cited: cocoa butter, coconut oil, horse oil, hydrogenated coconut oil, palm oil, beef tallow, mutton tallow, hydrogenated beef tallow, palm kernel oil, lard, beef bone fat (oil), wood wax kernel oil, hydrogenated oil, beef foot oil, wood wax, hydrogenated castor oil, hydrogenated palm oil, etc.

[0090] As waxes, for example, the following can be cited: beeswax, candelilla wax, cotton wax, carnauba wax, myrica wax, insect wax (white wax), spermaceti wax, lignite wax, rice bran wax, lanolin, kapok wax, acetylated lanolin, liquid lanolin, sugarcane wax, isopropyl lanolate, hexyl laurate, reduced lanolin, jojoba wax, hard lanolin, shellac wax, POE lanolin alcohol ether, POE lanolin alcohol acetate, POE cholesterol ether, polyethylene glycol lanolate, POE hydrogenated lanolin alcohol ether, etc.

[0091] As hydrocarbon oils, for example, the following can be cited: liquid paraffin, ozokerite, squalane, pristane, paraffin wax, pure ozokerite, squalene, petrolatum, microcrystalline wax, etc.

[0092] As higher fatty acids, for example, the following can be cited: lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid, oleic acid, undecenoic acid, tall oil fatty acid, isostearic acid, linoleic acid, linolenic acid, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), etc.

[0093] As higher alcohols, for example, the following can be cited: straight-chain alcohols (e.g., lauryl alcohol, cetyl alcohol, stearyl alcohol, behenyl alcohol, myristyl alcohol, oleyl alcohol, cetearyl alcohol, etc.); branched-chain alcohols (e.g., monostearyl glycerol ether (batyl alcohol), 2-decyltetradecanol, lanolin alcohol, cholesterol, phytosterol, hexyl dodecanol, isostearyl alcohol, octyl dodecanol, etc.).

[0094] Examples of the synthetic ester oil include: hydrogenated polydecene, isopropyl myristate, cetyl octanoate, octyldodecyl myristate, isopropyl palmitate, butyl stearate, hexyl laurate, myristyl myristate, decyl oleate, hexyl decyl dimethyl octanoate, cetyl lactate, myristyl lactate, lanolin acetate, isocetyl stearate, isocetyl isostearate, cholesteryl 12-hydroxystearate, ethylene glycol di-2-ethylhexanoate, dipentaerythritol fatty acid ester, N-alkyl ethylene glycol mono-isostearate, neopentyl glycol didecanoate, diisostearyl malate, glycerol tri-2-heptylundecanoate, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaerythritol tetra-2-ethylhexanoate, glycerol tri-2-ethylhexanoate, glycerol trioctanoate, glycerol tripalmitoleate, trimethylolpropane triisostearate, cetyl alcohol ethylhexanoate, 2-ethylhexyl palmitate, glycerol trimyristate, glycerol tri-2-heptylundecanoate, methyl ricinoleate, oleyl oleate, acetyl glyceride, 2-heptylundecyl palmitate, diisobutyl adipate, 2-octyldodecyl N-lauroyl-L-glutamate, di-2-heptylundecyl adipate, ethyl laurate, di-2-ethylhexyl sebacate, 2-hexyl decyl myristate, 2-hexyl decyl palmitate, 2-hexyl decyl adipate, diisopropyl sebacate, 2-ethylhexyl succinate, triethyl citrate, pentaerythritol tetra(ethylhexanoate), etc.

[0095] Examples of the silicone oil include: polydimethylsiloxane, methylhydrogenpolysiloxane, methylphenylpolysiloxane, stearyloxymethylpolysiloxane, polyether-modified organopolysiloxane, fluoroalkyl·polyalkylene oxide copolymer-modified organopolysiloxane, alkyl-modified organopolysiloxane, end-modified organopolysiloxane, fluoro-modified organopolysiloxane, amino-modified organopolysiloxane, PEG-10 polydimethylsiloxane, silica gel, acrylic polysiloxane, trimethylsilyloxy silicate, silicone compounds such as organosilicon RTV rubber, etc.

[0096] As the oil component used in combination with hydrogenated polyisobutene, an oil component having poor compatibility with hydrogenated polyisobutene and being volatile is preferred. In this way, during application, the volatile oil component will volatilize, and due to the poor compatibility, hydrogenated polyisobutene will remain on the skin to form a film, bringing a tightening feeling.

[0097] As anionic surfactants, examples include: fatty acid soaps (e.g., sodium laurate, sodium palmitate, etc.); higher alkyl sulfates (e.g., sodium lauryl sulfate, potassium lauryl sulfate, etc.); alkyl ether sulfates (e.g., triethanolamine POE-lauryl sulfate, sodium POE-lauryl sulfate, etc.); N-acyl sarcosines (e.g., sodium lauroyl sarcosinate, etc.); higher fatty acid amide sulfonates (e.g., sodium N-myristoyl-N-methyl taurate, sodium methyl taurate of coconut oil fatty acid, sodium lauryl methyl taurate, etc.); phosphate esters (sodium POE-oleyl ether phosphate, POE-stearyl ether phosphate, etc.); sulfosuccinates (e.g., sodium di-2-ethylhexyl sulfosuccinate, sodium monolauroyl monoethanolamide polyoxyethylene sulfosuccinate, sodium lauryl polypropylene glycol sulfosuccinate, etc.); alkylbenzene sulfonates (e.g., sodium linear dodecylbenzene sulfonate, triethanolamine linear dodecylbenzene sulfonate, linear dodecylbenzene sulfonic acid, etc.); higher fatty acid ester sulfates (e.g., sodium hydrogenated coconut oil fatty acid glyceride sulfate, etc.); N-acyl glutamates (e.g., monosodium N-lauroyl glutamate, disodium N-stearyl glutamate, monosodium N-myristoyl-L-glutamate, etc.); sulfated oils (e.g., Turkey red oil, etc.); POE-alkyl ether carboxylic acids, POE-alkyl allyl ether carboxylates, α-olefin sulfonates, higher fatty acid ester sulfonates, secondary alcohol sulfates, higher fatty acid alkanolamide sulfates, sodium lauroyl monoethanolamide succinate, di-triethanolamine N-palmitoyl aspartate, sodium caseinate, potassium cetyl alcohol phosphate, etc.

[0098] As cationic surfactants, examples include: alkyltrimethylammonium salts (e.g., trimethylstearylammonium chloride, trimethyllaurylammonium chloride, etc.); alkylpyridinium salts (e.g., cetylpyridinium chloride, etc.); dialkyldimethylammonium salts such as distearyldimethylammonium chloride; poly(N,N”-dimethyl-3,5-methylenepiperidinium) chloride; alkyl quaternary ammonium salts; alkyl dimethylbenzylammonium salts; alkylisoquinolinium salts; dialkylmorpholinium salts; POE-alkylamines; alkylamine salts; polyamine fatty acid derivatives; pentanol fatty acid ester derivatives; benzalkonium chloride; benzethonium chloride, etc.

[0099] As amphoteric surfactants, examples include: imidazoline-based amphoteric surfactants (e.g., sodium 2-undecyl-N,N,N-(hydroxyethyl carboxymethyl)-2-imidazoline, disodium 2-cocoyl-2-imidazolinium hydroxide-1-carboxyethoxy, etc.); betaine-based surfactants (e.g., 2-heptadecyl-N-carboxymethyl-N-hydroxyethylimidazolinium betaine, lauryldimethylaminoacetic acid betaine, alkyl betaine, amide betaine, sulfobetaine, etc.).

[0100] Examples of the lipophilic nonionic surfactant include: sorbitan fatty acid esters (e.g., sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, sorbitan penta-2-ethylhexanoate diglyceride, sorbitan tetra-2-ethylhexanoate diglyceride, etc.); polyglycerol fatty acid glycerides (e.g., glycerol monocottonseedate, glycerol monoerucate, glycerol sesquioleate, glycerol monostearate, α,α'-oleoyl pyroglutamic acid glyceride, glycerol malate monostearate, etc.); propylene glycol fatty acid esters (e.g., propylene glycol monostearate, etc.); hydrogenated castor oil derivatives; glycerol alkyl ethers; PEG-10 polydimethylsiloxane, glycerol stearate, PPG-13-decyltetradeceth-24, PEG-5 glycerol stearate, etc.

[0101] As hydrophilic nonionic surfactants, examples include: POE-sorbitan fatty acid esters (e.g., POE-sorbitan monooleate, POE-sorbitan monostearate, POE-sorbitan monooleate, POE-sorbitan tetraoleate, etc.); POE-sorbitol fatty acid esters (e.g., POE-sorbitol monolaurate, POE-sorbitol monooleate, POE-sorbitol pentaoleate, POE-sorbitol monostearate, etc.); POE-glycerol fatty acid esters (e.g., POE-glycerol monostearate, POE-glycerol monoisostearate, POE-glycerol triisostearate, etc., such as POE-monooleate); POE-fatty acid esters (e.g., POE-distearate, POE-mono dioleate, ethylene glycol distearate, etc.); POE-alkyl ethers (e.g., POE-lauryl ether, POE-oleyl ether, POE-stearyl ether, POE-behenyl ether, POE-2-octyldodecyl ether, POE-cholesteryl ether, etc.); Pluronic type (e.g., Pluronic, etc.); POE·POP-alkyl ethers (e.g., POE·POP-cetyl ether, POE·POP-2-decyltetradecyl ether, POE·POP-monobutyl ether, POE·POP-hydrogenated lanolin, POE·POP-glycerol ether, etc.); tetra-POE·tetraPOP-ethylenediamine condensate (e.g., Tetronic, etc.); POE-castor oil hydrogenated castor oil derivatives (e.g., POE-castor oil, POE-hydrogenated castor oil, POE-hydrogenated castor oil monoisostearate, POE-hydrogenated castor oil triisostearate, POE-hydrogenated castor oil monopyroglutamic acid monoisostearate diester, POE-hydrogenated castor oil maleate, etc.); POE-beeswax·lanolin derivatives (e.g., POE-sorbitol beeswax, etc.); alkanolamides (e.g., coconut oil fatty acid diethanolamide, lauric acid monoethanolamide, fatty acid isopropanolamide, etc.); POE-propylene glycol fatty acid esters; POE-alkylamines; POE-fatty acid amides; sucrose fatty acid esters; alkyl ethoxydimethylamine oxides; trioleyl phosphate; oleth-10, PEG-100 stearate, methoxy PEG / PPG-25 / 4 polydimethylsiloxane, polysorbate-60, PEG-40 stearate, sucrose stearate, behenyl ether. As natural water-soluble polymers, examples include: plant polymers (e.g., gum arabic, tragacanth, galactan, guar gum, carob gum, karaya gum, carrageenan, pectin, agar, quince seed, algal colloids (brown algae extract), starch (rice, corn, potato, wheat), glycyrrhizic acid); microbial polymers (e.g., xanthan gum, dextran, succinoglycan, pullulan, etc.); animal polymers (e.g., collagen, casein, albumin, gelatin, etc.).

[0102] As semi-synthetic water-soluble polymers, examples include: starch polymers (e.g., carboxymethyl starch, methylhydroxypropyl starch, etc.); cellulose polymers (methylcellulose, ethylcellulose, methylhydroxypropyl cellulose, hydroxyethyl cellulose, sodium cellulose sulfate, hydroxypropyl cellulose, carboxymethyl cellulose, sodium carboxymethyl cellulose, crystalline cellulose, cellulose powder, etc.); alginic acid polymers (e.g., sodium alginate, propylene glycol alginate, etc.), PEG-240 / HDI copolymer bis-decyltetradeceth-20, acrylate / C10-30 alkyl acrylate cross-linked polymer, etc.

[0103] As thickeners, examples include carbomer, acrylate / C10-30 alkyl acrylate cross-linked polymer, sodium acrylate / sodium acryloyldimethyltaurate copolymer, acrylate / ceteth(20) itaconate copolymer, acrylate / ceteth(20) methacrylate copolymer, acrylate / tetradeceth(25) acrylate copolymer, acrylate / ceteth(20) itaconate copolymer, acrylate / ceteth(20) methacrylate copolymer, acrylate / ceteth(50) acrylate copolymer, acrylate / VA cross-linked polymer, PAA (polyacrylic acid), sodium acrylate / vinyl isodecanoate cross-linked polymer, Carbomer (polyacrylic acid) and its sodium salts and other polyacrylic acid thickeners.

[0104] As preservatives, examples include aromatic preservatives such as phenoxyethanol, benzyl alcohol, methylparaben, p-hydroxyacetophenone, etc.

[0105] As metal ion sequestering agents, examples include: 1-hydroxyethane-1,1-diphosphonic acid, tetrasodium 1-hydroxyethane-1,1-diphosphonate, disodium edetate (EDTA2 sodium), trisodium edetate (EDTA3 sodium), tetrasodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, phosphoric acid, citric acid, ascorbic acid, succinic acid, edetic acid, trisodium ethylenediamine hydroxyethyltriacetate, etc.

[0106] As amino acids, examples include: neutral amino acids (e.g., threonine, cysteine, etc.); basic amino acids (e.g., hydroxylysine, etc.). In addition, as amino acid derivatives, examples include: sodium acylsarcosinate (sodium lauroylsarcosinate), acylglutamate, sodium acyl-β-alanine, glutathione, pyrrolidonecarboxylic acid, etc.

[0107] As organic amines, examples include: monoethanolamine, diethanolamine, triethanolamine, morpholine, triisopropanolamine, 2-amino-2-methyl-1,3-propanediol, 2-amino-2-methyl-1-propanol, etc.

[0108] As polymer emulsions, examples include: acrylic resin emulsion, polyethyl acrylate emulsion, acrylic resin solution, polyalkyl acrylate emulsion, polyvinyl acetate resin emulsion, natural rubber latex, etc.

[0109] As pH regulators, examples include: buffers such as lactic acid - sodium lactate, citric acid - sodium citrate, succinic acid - sodium succinate, etc.

[0110] As neutralizing agents, potassium hydroxide, sodium hydroxide, aminomethyl propanol, arginine, etc. can be cited.

[0111] As vitamins, examples include: vitamin A, B1, B2, B6, C, E and their derivatives, pantothenic acid and its derivatives, biotin, etc.

[0112] As antioxidants, examples include: tocopherols, dibutylhydroxytoluene, butylhydroxyanisole, gallate esters, sodium metabisulfite, etc.

[0113] As antioxidant aids, examples include: phosphoric acid, citric acid, ascorbic acid, maleic acid, malonic acid, succinic acid, fumaric acid, cephalin, hexametaphosphate (ester), phytic acid, ethylenediaminetetraacetic acid, etc.

[0114] Examples

[0115] Examples and test examples are listed below to illustrate the present invention in more detail, but the present invention is not limited to these examples.

[0116] 1. Preparation of polygonatum odoratum extract

[0117] Add polygonatum odoratum powder (20 g) to a beaker, add 500 mL of water / ethanol mixture (volume ratio of water:ethanol is 1:1), perform ultrasonic stirring, heat and extract at 89 °C for 6 hours, and then concentrate and dry to obtain polygonatum odoratum extract.

[0118] Dissolve the obtained polygonatum odoratum extract in a mixed solvent of water / butanediol (volume ratio of water:butanediol is 9:1), add activated carbon for decolorization and deodorization, then stand at low temperature of 6 - 8 °C for 48 hours, filter to remove insoluble substances, and add preservatives to obtain a semi - finished polygonatum odoratum extract solution.

[0119] Take a sample from the semi - finished polygonatum odoratum extract solution and send it to the quality control department for testing. Visually, the color is a yellow transparent liquid with a characteristic odor; the pH is in the range of 4 - 7, the polysaccharide content is greater than 0.2%, and the microbial and heavy metal indexes meet the requirements of the "Cosmetics Safety and Technical Standards". The polygonatum odoratum extract prepared in this way is a qualified product.

[0120] 2. Test examples

[0121] Test on the promotion of the production of tight junction proteins by polygonatum odoratum extract

[0122] Test drugs, materials, instruments and preparations: Human skin keratinocytes (HaCaT); DMEM medium (GIBCO, 500 ml); Fetal bovine serum (GIBCO, 500 ml); Trypsin-EDTA (GIBCO, 100 ml); Human Claudin-1 enzyme-linked immunosorbent assay (ELISA) kit (Dogesce); RTCA instrument (ACEA); Cell detection plate (E-plate16); Inverted microscope (OLYMPUS IX73); Multifunctional microplate reader (TECAN, Infinite M200 Pro); Incubator (Thermoscientific, DIRECT HEAT CO2 Incubator); Centrifuge (eppendorf, Centrifuge5810R); T25 cell culture flask (COSTAR, 430168); Cell culture plate (96-well) (Costar, 3599); Cell counter (Life, Countess II FL).

[0123] Test implementation conditions: Sterile, ultra-clean, and cells need to be cultured at 5% CO 2 and 37 °C.

[0124] Test implementation:

[0125] 1. Store the polygonatum extract at room temperature for later use. When in use, take out the sample and shake well, and dilute it with serum-free medium in gradients.

[0126] 2. Cell culture. Select HaCaT in the logarithmic growth phase, digest it with 0.25% trypsin, passage it with DMEM medium containing 10% fetal bovine serum, and place it in the incubator at 37 °C, 5% CO 2 environment for culture.

[0127] 3. Select HaCaT with a growth density of about 90% for the experiment, seed it into the E-plate16 plate for culture. After 24 h, dilute the polygonatum extract with DMEM medium according to the specified concentration and add it to the E-plate16 plate for continued culture. Observe after 24 h, and select the optimal concentration for subsequent experiments.

[0128] 4. Select HaCaT with a growth density of about 90% for the experiment, seed it into the 96-well plate for culture. After 24 h, dilute the polygonatum extract with DMEM medium according to the selected concentration and add it to the 96-well plate for continued culture. After 24 h, perform the test experiment on the ability to promote Claudin-1 production according to the operation steps in the instruction manual. Test results and discussion:

[0129] Expression of tight junction protein Claudin-1

[0130] As Figure 1 shown by the test results, within the preset concentration range, in the test of promoting Claudin-1 production by Polygonatum odoratum extract, the content of Claudin-1 was 10.87 ng / ml at a concentration of 0.1%, 10.78 ng / ml at a concentration of 0.05%, and 9.2779 ng / ml at a concentration of 0.01%. The result of the negative control DMEM was 9.571 ng / mL. It can be seen that the effect of promoting Claudin-1 expression by Polygonatum odoratum extract at a concentration of 0.01% is weaker than that of the negative control, and the effect is not good; Polygonatum odoratum extracts at concentrations of 0.05% and 0.1% can significantly promote the production of Claudin-1 protein in skin cells, thus playing the role of maintaining the skin barrier and moisturizing.

[0131] Example 1. Cream

[0132] According to the following formula, a cream containing Polygonatum odoratum extract as a skin barrier repair active ingredient was prepared by a conventional cosmetic manufacturing process.

[0133]

[0134] Effect Test Example 1

[0135] Evaluation experiment of transepidermal water loss (TEWL) of the skin

[0136] Transepidermal water loss (TEWL) of the skin is measured by Tewameter, TM300, and it is an important parameter reflecting skin water loss and barrier function. The lower the measured value, the better the skin barrier function.

[0137] For 28 subjects, after cleansing the face and applying lotion, an appropriate amount of the cream of Example 1 was gently applied. Twice a day, once in the morning and once in the evening, for 28 consecutive days. The following measurements were taken from the start of using the product until the 28th day of the test.

[0138] (1) Analysis of transepidermal water loss of the skin

[0139] Transepidermal water loss (TEWL) of the skin is measured by Tewameter, TM300, three times, and the average value is taken. The measurement site is the bilateral cheek area. Transepidermal water loss is an important parameter reflecting skin water loss and barrier function. The lower the measured value, the better the skin barrier function.

[0140] Data analysis was performed using SPSS 21.0 statistical software. For measurement data, a normal distribution test was conducted. If the p value of the normal distribution test > 0.05, it indicated that the data was normally distributed, and paired t-tests were used to analyze the within-group differences in the baseline values (D0) and follow-up values (D28) of each group's experimental parameters. Otherwise, the Wilcoxon signed-rank test was used. Meanwhile, independent sample t-tests or Wilcoxon rank-sum tests were used to analyze the between-group differences in the changes in the experimental parameters of each group (D28 - D0). At the D28 follow-up, a binomial test was used to analyze the differences in the degree of agreement in the self-assessment questionnaires of the subjects. Statistically significant differences were marked using p < 0.05*, p < 0.01**, and p < 0.001***.

[0141] Test results: Compared with the baseline value (D0), the transepidermal water loss rate of the cheek skin decreased by 14.42% at the D28 follow-up. Compared with D0, there was a significant difference in the transepidermal water loss rate of the cheek skin at the D28 follow-up (p < 0.001).

[0142] (2) Adverse reaction evaluation: A dermatologist conducted a clinical assessment of both sides of the face.

[0143] During the test process, adverse reaction observation and evaluation were carried out twice, respectively at D0 (before using the product) and D28 (the 28th day after using the product) when the subjects visited. The dermatologist asked the subjects whether they had symptoms such as facial skin itching, stinging, and burning during the use of the test product, and observed and recorded whether there were adverse reaction phenomena such as erythema, edema, and rash on the face. Scoring was performed according to the scoring criteria in Table 1, and the scores were recorded in the case report form, and a relevance assessment was carried out according to the criteria listed in Table 2.

[0144] Table 1 Scoring criteria for adverse reactions

[0145]

[0146] Table 2 Evaluation of the test product

[0147]

[0148] Test results: During the test process, no adverse reactions were observed in any of the subjects.

[0149] (3) Self-assessment questionnaire: The subjects self-assessed both sides of the face.

[0150] A total of 28 subjects completed the evaluation questionnaire on the usage experience and effects of the test product during the D28 follow-up visit. The options were given by the subjects based on their own feelings and with reference to the degree option criteria. The usage experience and effects of the test product were evaluated by the subjects using four degree options A - D (A indicates agreeing with the described usage experience or the effects of the test product; B indicates relatively agreeing with the described usage experience or the effects of the test product; C indicates not quite agreeing with the described usage experience or the effects of the test product; D indicates disagreeing with the described usage experience or the effects of the test product).

[0151] The test results are as follows:

[0152] 96.43% of the subjects believed that after using the test product, they felt an improvement in skin smoothness,

[0153] 96.43% of the subjects believed that after using the test product, they felt an increase in toughness (examples of toughness: being able to resist skin problems brought about by seasonal environmental changes),

[0154] 96.43% of the subjects believed that after using the test product, they felt an improvement in skin roughness,

[0155] 96.43% of the subjects believed that after using the test product, they felt an improvement in skin flushing,

[0156] 96.43% of the subjects believed that after using the test product, they felt an improvement in skin moisture.

[0157] From the above test results, it can be seen that the polygonatum extract can effectively promote the generation of tight junction proteins and is effective for the repair of the skin barrier.

[0158] Industrial availability

[0159] The present inventor first discovered that the polygonatum extract has the effect of promoting the generation of tight junction protein (Claudin-1) and verified the effect. Therefore, the polygonatum extract can be used as a promoter for the generation of tight junction proteins to promote the generation of tight junction proteins in the skin, and thus promote the repair of the skin barrier. Therefore, the polygonatum extract can be applied to cosmetics that require the function of repairing the skin barrier.

Claims

1. A tight junction protein (Claudin-1) production promoter, characterized in that Consists of polygonatum odoratum extract only.

2. A skin barrier repair agent, characterized in that: Contains polygonatum odoratum extract.

3. Use of Polygonatum odoratum extract as a promoter for the production of tight junction protein (Claudin-1).

4. Use of Polygonatum odoratum extract for preparing a tight junction protein (Claudin-1) production promoter.

5. Use of Polygonatum odoratum extract in preparing cosmetics for promoting the production of tight junction proteins.

6. Use of Polygonatum odoratum extract in preparing cosmetics for promoting skin barrier repair.

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