Micro-capsule powder composition containing xanthoceras sorbifolia bunge oil as well as preparation method and application of micro-capsule powder composition

Abstract

The invention discloses a micro-capsule powder composition containing xanthoceras sorbifolia bunge oil as well as a preparation method and application of the micro-capsule powder composition, and belongs to the technical field of biological medicines. The micro-capsule powder composition containing the xanthoceras sorbifolia bunge oil comprises xanthoceras sorbifolia bunge oil micro-capsule powder and traditional Chinese medicine micro-capsule powder, wherein the mass ratio of the xanthoceras sorbifolia bunge oil micro-capsule powder to the traditional Chinese medicine micro-capsule powder is (4-8): 6; the traditional Chinese medicine micro-capsule powder comprises the following active ingredients in parts by weight: 10-13 parts of ginseng, 10-12 parts of polygala tenuifolia, 10-12 parts of poria cocos, 10 parts of poria with hostwood, 5-6 parts of rhizoma acori graminei and 5 parts of spina date seeds. After the xanthoceras sorbifolia bunge oil micro-capsule powder and the traditional Chinese medicine micro-capsule powder are compounded, the effect of the xanthoceras sorbifolia bunge oil micro-capsule powder on improvement of the Alzheimer's disease is improved, and the improvement effect is very remarkable.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomedicine, and relates to a microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil, a preparation method thereof, and an application thereof. Background Art

[0002] Alzheimer's Disease (AD) is a progressive neurodegenerative disease, characterized by progressive decline in memory and cognitive function and behavioral changes, and clinically also known as dementia. The pathogenesis of AD is complex and not fully understood yet, and the main pathological markers of AD are the formation and deposition of amyloid plaques in the brain and neuronal degeneration and death. Currently, there is no specific medicine for treating Alzheimer's disease, and the clinical treatment methods mainly focus on symptomatic treatment, which can only relieve symptoms temporarily and cannot reverse or prevent the deterioration of the disease.

[0003] Traditional Chinese medicines contain various bioactive components, such as polyphenols, flavonoids, saponins, and alkaloids, etc., and have been proven to have effects such as anti-aging, antioxidant, neuroprotection, and improvement of learning and memory. These components can improve symptoms such as aging, oxidative stress, Aβ amyloid deposition, and learning and memory disorders in AD. Therefore, the characteristics of traditional Chinese medicines of multiple components, multiple targets, and multiple pathways make them show unique advantages in regulating the body balance and treating complex diseases.

[0004] Xanthoceras sorbifolia Bunge Xanthoceras sorbifolium ), belonging to the genus Xanthoceras of the family Sapindaceae, is a woody oil tree species that can be used as both medicine and food. The oil content of its kernels is about 60% or so, mainly unsaturated fatty acids. In addition, Xanthoceras sorbifolia Bunge oil contains rich nervonic acid, with a content of about 3.0%. This special functional component endows Xanthoceras sorbifolia Bunge oil ( Xanthoceras sorbifolium oil , XSO) with various biological activities, such as reducing blood lipid, preventing cardiovascular diseases, anti-inflammatory, antioxidant, and anti-aging. As a key component of biological membranes, nervonic acid plays a crucial role in the formation of nerve fiber myelin sheaths and the conduction of nerve signals, and is of great significance for maintaining the normal structural function of the nervous system and preventing neurodegenerative diseases. Therefore, Xanthoceras sorbifolia Bunge oil containing nervonic acid has high medical and medicinal values. Summary of the Invention

[0005] The purpose of the present invention is to solve the problems that the stability of Xanthoceras sorbifolia Bunge kernels is poor, they are easy to oxidize and deteriorate, affecting their quality and efficacy, etc. The present invention provides a microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil, a preparation method thereof, and an application thereof, which improves the stability and bioavailability of Xanthoceras sorbifolia Bunge oil.

[0006] Although some specific Chinese medicine combinations have been reported to improve Alzheimer's disease, there are differences in the therapeutic effects of different prescriptions. In addition, how to optimize the clinical application experience of Chinese medicine prescriptions and improve their therapeutic effects is still one of the technical problems that need to be overcome.

[0007] In a first aspect, the present invention provides a microcapsule powder composition containing Xanthoceras sorbifolia oil, which is composed of Xanthoceras sorbifolia oil microcapsule powder and traditional Chinese medicine microcapsule powder; In parts by weight, the Chinese medicinal raw materials in the Chinese medicinal microcapsule powder are composed of 10-13 parts of ginseng, 10-12 parts of polygala, 10-12 parts of tuckahoe, 10 parts of poria, 5-6 parts of calamus and 5 parts of spinach seeds.

[0008] Based on the previous test results, the present invention provides a Chinese medicine compatibility formula, which specifically includes the following Chinese medicine ingredients: ginseng, polygala, tuckahoe, poria, calamus and spiny jujube seeds. Ginseng, polygala, tuckahoe, poria, calamus and spiny jujube seeds are six commonly used medicinal materials in traditional Chinese medicine, each of which has different effects and functions. For example, ginseng tastes sweet and slightly bitter, is slightly warm in nature, and enters the spleen, lung, heart and kidney meridians, has strong qi and moistens the body, and has more ascending than descending; it has the effects of replenishing qi and consolidating deficiency, strengthening the spleen and lungs, calming the mind and improving intelligence, and nourishing blood and producing body fluid. Polygala enters the heart, kidney and lung meridians. It has the effects of calming the mind and improving intelligence, connecting the heart and kidney, removing phlegm and reducing swelling, and is used for insomnia, dreaminess, forgetfulness, palpitations and confusion caused by heart-kidney disharmony. Poria enters the heart and spleen meridians, and has the effects of calming the mind and calming the nerves, replenishing deficiency and treating fatigue, promoting diuresis, moistening the intestines and relieving constipation, and draining water and dampness. Acorus calamus belongs to the heart and stomach meridians, and has the effects of opening the orifices and removing phlegm, refreshing the mind and improving intelligence, removing dampness and stimulating appetite. It is used for coma, epilepsy, forgetfulness, insomnia, tinnitus, deafness, abdominal distension without hunger, and dysentery. Ziziphus jujuba seeds belong to the heart, spleen, liver, and gallbladder meridians, and are used for palpitations and insomnia caused by blood deficiency and inability to nourish the heart or virtual fire inflammation. These medicinal materials have an important position in the theory and practice of traditional Chinese medicine, but their use also has certain restrictions and precautions, such as dosage, compatibility, decoction, etc.

[0009] Furthermore, in the microcapsule powder composition containing Xanthoceras sorbifolia oil provided by the present invention, the particle size of the Xanthoceras sorbifolia oil microcapsule powder is 10-40 μm, and the particle size of the traditional Chinese medicine microcapsule powder is 3-11 μm.

[0010] Furthermore, in the microcapsule powder composition containing Xanthoceras sorbifolia oil provided by the present invention, the ratio of the Xanthoceras sorbifolia oil microcapsule powder to the traditional Chinese medicine microcapsule powder is 4-8:6 by mass ratio.

[0011] Furthermore, in the microcapsule powder composition containing Xanthoceras sorbifolia oil provided by the present invention, the ratio of the Xanthoceras sorbifolia oil microcapsule powder to the traditional Chinese medicine microcapsule powder is 4:6 by mass ratio.

[0012] In a second aspect, the present invention provides a method for preparing a microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil, comprising: emulsifying the Xanthoceras sorbifolia Bunge kernel oil and then encapsulating it with a wall material to obtain the Xanthoceras sorbifolia Bunge oil microcapsule powder; Subjecting the traditional Chinese medicine raw materials to alcohol extraction and / or water extraction and then concentrating to obtain a traditional Chinese medicine extract, mixing the traditional Chinese medicine extract with water, and encapsulating it with a wall material to obtain the traditional Chinese medicine microcapsule powder.

[0013] Further, in the method for preparing the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil provided by the present invention, the emulsifier used for emulsification is Tween 80; The wall material used for the Xanthoceras sorbifolia Bunge oil microcapsule powder is gelatin and sucrose; The wall material used for the traditional Chinese medicine microcapsule powder is gelatin and sucrose.

[0014] Further, in the method for preparing the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil provided by the present invention, the traditional Chinese medicine extract is mixed with water at a ratio of 10 - 30 g:100 mL.

[0015] Further, in the method for preparing the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil provided by the present invention, the mass ratio of gelatin to sucrose in the Xanthoceras sorbifolia Bunge oil microcapsule powder is 1:3 - 6; The mass ratio of gelatin to sucrose in the traditional Chinese medicine microcapsule powder is 5:1 - 3.

[0016] In a third aspect, the present invention provides the use of a microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil in the preparation of drugs for Alzheimer's disease.

[0017] Further, in the use of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil in the preparation of drugs for Alzheimer's disease provided by the present invention, the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil improves at least one of lifespan, olfactory memory, the expression level of brain tissue Aβ42 protein, and antioxidant capacity.

[0018] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages: (1) The stability of Xanthoceras sorbifolia Bunge oil is poor and it is prone to oxidation and deterioration, which affects its quality and efficacy. The microencapsulation technology can protect the internal substances from the external environment, extend its shelf life and effective period; and can improve the stability and bioavailability of Xanthoceras sorbifolia Bunge oil.

[0019] (2) Preparing the traditional Chinese medicine extract into microcapsule powder has good fluidity, is easy to disperse and dissolve; can improve the solubility and absorption rate of vegetable oil and traditional Chinese medicine; simplifies the taking method of vegetable oil and traditional Chinese medicine; and can reduce the taste and smell of vegetable oil and traditional Chinese medicine.

[0020] (3) Using gelatin plus sucrose as the wall material to encapsulate vegetable oil or traditional Chinese medicine can improve the stability and bioavailability of vegetable oil or traditional Chinese medicine.

[0021] (4)After the microcapsule powder of Xanthoceras sorbifolia Bunge oil and the microcapsule powder of the traditional Chinese medicine formula extract are proportioned, the symptoms of Alzheimer's disease can be significantly improved, and there are no obvious side effects.

[0022] (5)The present invention adopts preparation forms such as capsules, tablets, pills, etc., which can effectively relieve the difficulty of taking medicine caused by the discomfort of the smell for patients.

[0023] (6)The present invention uses transgenic Drosophila with Alzheimer's disease as an experimental model animal to explore the effect of the traditional Chinese medicine composition on improving Alzheimer's disease. The research results show that after the treatment with the traditional Chinese medicine composition, at the effective dose of a specific dose, the symptoms of Alzheimer's disease can be significantly improved. Description of the Drawings

[0024] Figure 1 It is the effect of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil on the lifespan of AD Drosophila. Among them, A is the survival rate of Drosophila in the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil at a dose of 5 g / kg, the control group and the AD model group; B is the survival rate of Drosophila in the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil at a dose of 10 g / kg, the control group and the AD model group; C is the survival rate of Drosophila in the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil at a dose of 20 g / kg, the control group and the AD model group; D is the survival rate of Drosophila in the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil at a dose of 40 g / kg, the control group and the AD model group; E is the lifespan of Drosophila in the control group, the AD model group and the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil; W1118 is the control group; AD is the AD model group; 5g / kgAD-XSO is the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil at a dose of 5 g / kg; 10g / kgAD-XSO is the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil at a dose of 10 g / kg; 20g / kgAD-XSO is the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil at a dose of 20 g / kg; 40g / kgAD-XSO is the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil at a dose of 40 g / kg; * represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001.

[0025] Figure 2 It is the effect of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil on the olfactory memory of AD Drosophila. Among them, W1118 is the control group; AD is the AD model group; AD-XSO is the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil; * represents P < 0.05, ** represents P < 0.01, *** represents P < 0.001, ns represents non-statistical significance.

[0026] Figure 3Effect of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil on the expression level of Aβ42 protein in the brain tissue of AD Drosophila. Among them, A is the expression level of soluble Aβ42 protein; B is the expression level of insoluble Aβ42 protein; W1118 is the control group; AD is the AD model group; AD-XSO is the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil; *** indicates P < 0.001.

[0027] Figure 4 Effect of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil on the antioxidant capacity of AD Drosophila. Among them, A is the content of superoxide dismutase; B is the content of glutathione peroxidase; W1118 is the control group; AD is the AD model group; AD-XSO is the intervention group of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil; *** indicates P < 0.001. Detailed implementation manners

[0028] Next, the technical solutions of the present invention will be described in conjunction with the embodiments. However, the present invention is not limited to the following embodiments. The experimental methods and detection methods described in each embodiment are all conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0029] Example 1 This example provides a microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil and its preparation method.

[0030] 1. The microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil in this example includes Xanthoceras sorbifolia Bunge oil microcapsule powder and traditional Chinese medicine microcapsule powder, and the mass ratio of Xanthoceras sorbifolia Bunge oil microcapsule powder to traditional Chinese medicine microcapsule powder is 4:6; The active ingredient of the Xanthoceras sorbifolia Bunge oil microcapsule powder contains: Xanthoceras sorbifolia Bunge kernel oil The wall materials of the Xanthoceras sorbifolia Bunge oil microcapsule powder and the traditional Chinese medicine microcapsule powder contain: gelatin and sucrose.

[0031] 2. The preparation method of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil in this example is as follows: (1)The microcapsule powder of Xanthoceras sorbifolia Bunge oil was prepared by the emulsification method. The specific operations included: mixing Xanthoceras sorbifolia Bunge oil and emulsifier Tween 80 at a ratio of 300:1, homogenizing with a high-speed homogenizer at 50 °C and 12000 r / min for 15 minutes, and homogenizing 3 times at 45 MPa, 10 minutes each time, to form an emulsion; using a mixed solution of gelatin and sucrose as the wall material of the microcapsule powder of Xanthoceras sorbifolia Bunge oil. Among them, the mass ratio of gelatin to sucrose was 1:3. Gelatin and sucrose were dissolved in double-distilled water. The emulsion was mixed with the wall material, stirred with a stirrer at 11000 r / min for 10 minutes, and homogenized 3 times at 30 MPa, 8 minutes each time, and dispersed at high speed for 1 minute to obtain an emulsion with a solid content of 20%. The emulsion was spray-dried through a spray drying tower to obtain the microcapsule powder of Xanthoceras sorbifolia Bunge oil. Analyze the physicochemical properties of the microcapsule powder of Xanthoceras sorbifolia Bunge oil.

[0032] (2)The microcapsule powder of traditional Chinese medicine was prepared by the emulsification method. The specific operations included: mixing ginseng, polygala tenuifolia, poria cocos, Poria cum Radix Pini, acorus tatarinowii and wild jujube seeds and soaking them in 80% ethanol (the volume ratio of the traditional Chinese medicine mixture to ethanol was 1:9), heating under reflux for 1 h, repeating the extraction 3 times, and collecting the extracted ethanol extract and drug residues. The ethanol extract was concentrated at 75 °C at a ratio of 1.06 - 1.12 to obtain a concentrated ethanol extract. Water was added to the drug residues (the volume ratio of the drug residues to water was 1:8), heated under reflux for 2 h, repeated the extraction 3 times, and collected the extracted aqueous solution. The aqueous extract was concentrated at 90 °C at a ratio of 1.05 - 1.12 to obtain a concentrated aqueous extract. The concentrated ethanol extract and the concentrated aqueous extract were mixed and evaporated at 75 °C to obtain a crude extract of traditional Chinese medicine. The crude extract of traditional Chinese medicine was dissolved in water at a ratio of 10 g / 100 mL, gelatin and sucrose were added (the mass ratio of gelatin to sucrose was 5:1), stirred with a stirrer at 13000 r / min for 12 min, and homogenized 3 times at 30 MPa, 8 minutes each time, to form an emulsion. The emulsion was spray-dried through a spray drying tower to obtain the microcapsule powder of traditional Chinese medicine. Analyze the physicochemical properties of the microcapsule powder of traditional Chinese medicine.

[0033] (3)The preparation method of the composition containing the microcapsule powder of Xanthoceras sorbifolia Bunge oil included: mixing the microcapsule powder of Xanthoceras sorbifolia Bunge oil and the microcapsule powder of traditional Chinese medicine evenly according to a mass ratio of 4:6.

[0034] Example 2 This example provided a composition containing the microcapsule powder of Xanthoceras sorbifolia Bunge oil and its preparation method.

[0035] 1. The microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil in this example included the microcapsule powder of Xanthoceras sorbifolia Bunge oil and the microcapsule powder of traditional Chinese medicine, and the mass ratio of the microcapsule powder of Xanthoceras sorbifolia Bunge oil to the microcapsule powder of traditional Chinese medicine was 8:6; In parts by weight, the active ingredients in the traditional Chinese medicine microcapsule powder include: 10 parts of ginseng, 10 parts of polygala root, 10 parts of poria, 10 parts of tuckahoe, 5 parts of acorus tatarinowii, and 5 parts of wild jujube seed; The active ingredient of the Xanthoceras sorbifolium Bunge oil microcapsule powder includes: Xanthoceras sorbifolium Bunge kernel oil The wall materials of the Xanthoceras sorbifolium Bunge oil microcapsule powder and the traditional Chinese medicine microcapsule powder include: gelatin and sucrose.

[0036] 2. The preparation method of the composition containing Xanthoceras sorbifolium Bunge oil microcapsule powder in this embodiment is as follows: (1) Prepare Xanthoceras sorbifolium Bunge oil microcapsule powder by the emulsification method. The specific operations include: mixing Xanthoceras sorbifolium Bunge oil and emulsifier Tween 80 in a ratio of 300:1, homogenizing with a high-speed homogenizer at 50 °C and 12000 r / min for 15 minutes, and homogenizing 3 times at 45 MPa, 10 minutes each time, to form an emulsion; using a mixed solution of gelatin and sucrose as the wall material of the Xanthoceras sorbifolium Bunge oil microcapsule powder, wherein the mass ratio of gelatin to sucrose is 1:4. Dissolve gelatin and sucrose in double-distilled water. Mix the emulsion with the wall material, stir with a stirrer at 11000 r / min for 10 minutes, and homogenize 3 times at 30 MPa, 8 minutes each time, and disperse at high speed for 1 minute to obtain an emulsion with a solid content of 25%. Spray-dry the emulsion through a spray drying tower, with an inlet air temperature of 165 °C and an outlet air temperature of 80 °C, to obtain Xanthoceras sorbifolium Bunge oil microcapsule powder. Analyze the physicochemical properties of the Xanthoceras sorbifolium Bunge oil microcapsule powder.

[0037] (2) Prepare traditional Chinese medicine microcapsule powder by the emulsification method. The specific operations include: mixing ginseng, polygala root, poria, tuckahoe, acorus tatarinowii, and wild jujube seed, and soaking them in 80% ethanol (the volume ratio of the traditional Chinese medicine mixture to ethanol is 1:9), heating under reflux for 1 h, repeating the extraction 3 times, and collecting the extracted alcohol extract and drug residues. Concentrate the alcohol extract at 75 °C in a ratio of 1.06 - 1.12 to obtain a concentrated alcohol extract. Add water to the drug residues (the volume ratio of the drug residues to water is 1:8), heat under reflux for 2 h, repeat the extraction 3 times, collect the extracted aqueous solution, and concentrate the aqueous extract of traditional Chinese medicine at 90 °C in a ratio of 1.05 - 1.12 to obtain a concentrated aqueous extract. Mix the concentrated alcohol extract and the concentrated aqueous extract, and evaporate at 75 °C to obtain a crude traditional Chinese medicine extract. Dissolve the crude traditional Chinese medicine extract in water at a ratio of 30 g / 100 mL, add gelatin and sucrose (the mass ratio of gelatin to sucrose is 5:3), stir with a stirrer at 13000 r / min for 12 min, and homogenize 3 times at 30 MPa, 8 minutes each time, to form an emulsion. Similarly, spray-dry the emulsion through a spray drying tower to obtain traditional Chinese medicine microcapsule powder. Analyze the physicochemical properties of the traditional Chinese medicine microcapsule powder.

[0038] (3)The preparation method of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil includes: mixing Xanthoceras sorbifolia Bunge oil microcapsule powder and traditional Chinese medicine microcapsule powder evenly according to a mass ratio of 8:6.

[0039] Example 3 This example provides a microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil and its preparation method.

[0040] 1. The microcapsule powder of the traditional Chinese medicine composition containing Xanthoceras sorbifolia Bunge oil in this example includes Xanthoceras sorbifolia Bunge oil microcapsule powder and traditional Chinese medicine microcapsule powder, and the mass ratio of Xanthoceras sorbifolia Bunge oil microcapsule powder to traditional Chinese medicine microcapsule powder is 4:6; Calculated by weight, the active ingredients in the traditional Chinese medicine microcapsule powder include: 13 parts of ginseng, 12 parts of polygala root, 12 parts of poria, 10 parts of Poria cum Radix Pini, 6 parts of acorus tatarinowii, and 5 parts of wild jujube seed; The active ingredient of the Xanthoceras sorbifolia Bunge oil microcapsule powder includes: Xanthoceras sorbifolia Bunge kernel oil The wall materials of the Xanthoceras sorbifolia Bunge oil microcapsule powder and the traditional Chinese medicine microcapsule powder include: gelatin and sucrose.

[0041] 2. The preparation method of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil in this example is as follows: (1)Use the emulsification method to prepare Xanthoceras sorbifolia Bunge oil microcapsule powder. The specific operations include: mixing Xanthoceras sorbifolia Bunge oil and emulsifier Tween 80 in a ratio of 300:1, homogenizing with a high-speed homogenizer at 50 °C and 12000 r / min for 15 minutes, and homogenizing 3 times at 45 MPa, 10 minutes each time, to form an emulsion; use a mixed solution of gelatin and sucrose as the wall material of the Xanthoceras sorbifolia Bunge oil microcapsule powder, where the mass ratio of gelatin to sucrose is 1:5. Dissolve gelatin and sucrose with double-distilled water. Mix the emulsion with the wall material, stir with a stirrer at 11000 r / min for 10 minutes, and homogenize 3 times at 30 MPa, 8 minutes each time, and disperse at high speed for 1 minute to obtain an emulsion with a solid content of 25%. Spray-dry the emulsion through a spray drying tower to obtain Xanthoceras sorbifolia Bunge oil microcapsule powder. Analyze the physicochemical properties of the Xanthoceras sorbifolia Bunge oil microcapsule powder.

[0042] (2)The traditional Chinese medicine microcapsule powder is prepared by the emulsification method. The specific operations include: mixing ginseng, polygala root, poria cocos, poria with hostwood, acorus tatarinowii and wild jujube seed, and soaking them in 80% ethanol (the volume ratio of the traditional Chinese medicine mixture to ethanol is 1:9), heating under reflux for 1 h, repeating the extraction 3 times, and collecting the extracted ethanol solution and drug residue. The ethanol solution is concentrated at 75 °C in a ratio of 1.06 - 1.12 to obtain a concentrated ethanol extract. The drug residue is added with water (the volume ratio of the drug residue to water is 1:8), heated under reflux for 2 h, repeating the extraction 3 times, collecting the extracted aqueous solution, and concentrating the aqueous extract solution at 90 °C in a ratio of 1.05 - 1.12 to obtain a concentrated aqueous extract. The concentrated ethanol extract and the concentrated aqueous extract are mixed and evaporated at 75 °C to obtain a crude traditional Chinese medicine extract. The crude traditional Chinese medicine extract is dissolved in water at a ratio of 10 g / 100 mL, gelatin and sucrose are added (the mass ratio of gelatin to sucrose is 5:1), and stirred with a blender at 13000 r / min for 12 min, homogenized 3 times at 30 MPa, 8 min each time, to form an emulsion. Similarly, the emulsion is spray-dried through a spray drying tower to obtain the traditional Chinese medicine microcapsule powder. Analyze the physicochemical properties of the traditional Chinese medicine microcapsule powder.

[0043] (3)The preparation method of the composition containing Xanthoceras sorbifolia Bunge oil microcapsule powder includes: mixing the Xanthoceras sorbifolia Bunge oil microcapsule powder and the traditional Chinese medicine microcapsule powder evenly according to a mass ratio of 4:6.

[0044] Example 4 This example provides a composition containing Xanthoceras sorbifolia Bunge oil microcapsule powder and its preparation method.

[0045] 1. The microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil in this example includes Xanthoceras sorbifolia Bunge oil microcapsule powder and traditional Chinese medicine microcapsule powder, and the mass ratio of Xanthoceras sorbifolia Bunge oil microcapsule powder to traditional Chinese medicine microcapsule powder is 8:6; Calculated by weight, the active ingredients in the traditional Chinese medicine microcapsule powder include: 13 parts of ginseng, 12 parts of polygala root, 12 parts of poria cocos, 10 parts of poria with hostwood, 6 parts of acorus tatarinowii and 5 parts of wild jujube seed; The active ingredient of the Xanthoceras sorbifolia Bunge oil microcapsule powder includes: Xanthoceras sorbifolia Bunge kernel oil The wall materials of the Xanthoceras sorbifolia Bunge oil microcapsule powder and the traditional Chinese medicine microcapsule powder include: gelatin and sucrose.

[0046] 2. The preparation method of the composition containing Xanthoceras sorbifolia Bunge oil microcapsule powder in this example is as follows: (1)The microcapsule powder of Xanthoceras sorbifolia Bunge oil was prepared by the emulsification method. The specific operations included: mixing Xanthoceras sorbifolia Bunge oil and emulsifier Tween 80 at a ratio of 300:1, homogenizing with a high-speed homogenizer at 50 °C and 12,000 r / min for 15 minutes, and homogenizing 3 times at 45 MPa, 10 minutes each time, to form an emulsion; using a mixed solution of gelatin and sucrose as the wall material of the microcapsule powder of Xanthoceras sorbifolia Bunge oil, wherein the mass ratio of gelatin to sucrose was 1:6. Dissolve gelatin and sucrose using double-distilled water. Mix the emulsion with the wall material, stir with a blender at 11,000 r / min for 10 minutes, and homogenize 3 times at 30 MPa, 8 minutes each time, and disperse at high speed for 1 minute to obtain an emulsion with a solids content of 25%. Similarly, spray-dry the emulsion through a spray drying tower to obtain the microcapsule powder of Xanthoceras sorbifolia Bunge oil. Analyze the physicochemical properties of the microcapsule powder of Xanthoceras sorbifolia Bunge oil.

[0047] (2)The microcapsule powder of traditional Chinese medicine was prepared by the emulsification method. The specific operations included: mixing ginseng, polygala root, poria, Poria cum Radix Pini, acorus tatarinowii and wild jujube seed, and soaking them in 80% ethanol (the volume ratio of the traditional Chinese medicine mixture to ethanol was 1:9), heating under reflux for 1 h, repeating the extraction 3 times, and collecting the extracted ethanol extract and drug residue. Concentrate the ethanol extract at 75 °C at a ratio of 1.06 - 1.12 to obtain a concentrated ethanol extract. Add water to the drug residue (the volume ratio of the drug residue to water was 1:8), heat under reflux for 2 h, repeat the extraction 3 times, collect the extracted aqueous solution, and concentrate the aqueous extract of traditional Chinese medicine at 90 °C at a ratio of 1.05 - 1.12 to obtain a concentrated aqueous extract. Mix the concentrated ethanol extract and the concentrated aqueous extract, and evaporate at 75 °C to obtain a crude extract of traditional Chinese medicine. Dissolve the crude extract of traditional Chinese medicine in water at a ratio of 30 g / 100 mL, add gelatin and sucrose (the mass ratio of gelatin to sucrose was 5:3), stir with a blender at 13,000 r / min for 12 min, and homogenize 3 times at 30 MPa, 8 minutes each time, to form an emulsion. Similarly, spray-dry the emulsion through a spray drying tower to obtain the microcapsule powder of traditional Chinese medicine. Analyze the physicochemical properties of the microcapsule powder of traditional Chinese medicine.

[0048] (3)The preparation method of the composition containing the microcapsule powder of Xanthoceras sorbifolia Bunge oil included: mixing the microcapsule powder of Xanthoceras sorbifolia Bunge oil and the microcapsule powder of traditional Chinese medicine evenly according to a mass ratio of 8:6.

[0049] The physicochemical properties of the composition of the microcapsule powder of Xanthoceras sorbifolia Bunge oil and the microcapsule powder of traditional Chinese medicine described in Examples 1 - 4 are shown in Table 1.

[0050] Table 1 Physicochemical properties of the microcapsule powder of Xanthoceras sorbifolia Bunge oil and the microcapsule powder of traditional Chinese medicine

[0051] Example 5 This example provided the therapeutic effect of the composition containing the microcapsule powder of Xanthoceras sorbifolia Bunge oil on transgenic AD model Drosophila.

[0052] 1. Experimental design: Using the most commonly used Gal4 / UAS system in transgenic AD model Drosophila, newly born Elav-gal4 female Drosophila and UAS-Aβ42 male Drosophila were placed in a conical flask containing Drosophila medium for hybridization. On the 7th day of hybridization, the parental Drosophila in the medium were removed, and newly born Elav-gal4>>UAS-Aβ42 first-generation straight-wing male Drosophila within 3 days were selected, that is, the transgenic AD model Drosophila was successfully constructed.

[0053] The constructed transgenic AD model Drosophila were randomly divided into an AD model group and four intervention groups of compositions containing Xanthoceras sorbifolia Bunge oil microcapsule powder at different doses (5 g / kg, 10 g / kg, 20 g / kg, and 40 g / kg) (denoted as the Xanthoceras sorbifolia Bunge oil microcapsule powder composition intervention groups). At the same time, wild-type W1118 male Drosophila were selected as the control group. First, a lifespan experiment was carried out to screen out the optimal administration dose of the microcapsule powder composition containing Xanthoceras sorbifolia Bunge oil, and the optimal administration dose was used for intervention in subsequent experiments. The administration time was 21 days. A total of 3 groups were set in the subsequent experiments, namely the wild-type W1118 control group, the AD model group, and the Xanthoceras sorbifolia Bunge oil microcapsule powder composition intervention group (AD-XSO group).

[0054] 2. Experimental methods (1) Lifespan experiment Male Drosophila that emerged within 8 hours were collected, 100 in each group, and were respectively placed in media containing Xanthoceras sorbifolia Bunge oil microcapsule powder composition at 5 g / kg, 10 g / kg, 20 g / kg, and 40 g / kg, and cultured in an intelligent incubator at a temperature of (25±1) °C, a relative humidity of 50% - 60%, and a light cycle of 12 h. The number of dead Drosophila in each medium was observed and counted regularly every day. The medium was changed every 3 days until all Drosophila died, and a Drosophila survival curve was drawn.

[0055] (2) Olfactory memory experiment Male Drosophila of the wild-type W1118 control group, the AD model group, and the Xanthoceras sorbifolia Bunge oil microcapsule powder composition intervention group were collected, 20 in each group. After fasting the above 3 groups of Drosophila to be tested for 2 hours, they were transferred to a 1000 mL beaker. Two 50 mL centrifuge tubes with holes were pre-placed in the beaker, and bananas were placed in both. One of the centrifuge tubes was designed to allow Drosophila to enter through the holes and eat the bananas, while the other centrifuge tube did not allow Drosophila to enter through the holes and could not obtain bananas. The number of Drosophila entering the unobstructed centrifuge tube within 1 hour was recorded, and there were 3 parallels in each group.

[0056] 3. ELISA detection of the expression level of soluble Aβ42 protein in Drosophila brain tissue Collect 100 male Drosophila melanogaster in each of the wild-type W1118 control group, AD model group, and the intervention group with Xanthoceras sorbifolium Bunge oil microcapsule powder composition. CO 2 After anesthesia, quickly select the heads of Drosophila melanogaster on the ice surface. Put the Drosophila heads into centrifuge tubes, add 200 μL of protein lysis buffer (containing 2 μL of protease inhibitor and 2 μL of phosphatase inhibitor), and thoroughly grind the brain tissue evenly with a grinder. Centrifuge the homogenate at 4°C and 12,000 r / min for 10 min, and take the supernatant for detection. Operate according to the instructions of the human Aβ42 ELISA kit.

[0057] 4. Detection of the antioxidant capacity of Drosophila melanogaster brain tissue Collect 100 male Drosophila melanogaster in each of the wild-type W1118 control group, AD model group, and the intervention group with Xanthoceras sorbifolium Bunge oil microcapsule powder composition. CO 2 After anesthesia, quickly select the heads of Drosophila melanogaster on the ice surface. Put the Drosophila heads into centrifuge tubes, add 100 μL of ATP lysis buffer solution, and thoroughly grind the brain tissue evenly with a grinder. After lysis, centrifuge at 4°C and 12,000 r / min for 5 min, and take the supernatant for detection. Determine the activities of SOD and GSH-Px according to the operation instructions of the SOD detection kit and the GSH-Px detection kit. Measure the absorbance at 450 nm with an enzyme-labeling instrument, and calculate the activity of SOD according to the calculation formula of SOD.

[0058] 5. Statistics and analysis of experimental data Use GraphPad Prism 9X for graphing and data analysis. For comparisons among multiple groups, use one-way ANOVA (Ordinary one-way ANOVA) for multiple comparisons (Multiple comparisons) analysis. For pairwise comparisons between groups, use two-way ANOVA analysis. p < 0.001 indicates a significant difference with statistical significance. The results of the experimental data are expressed as mean ± standard error (mean ± SEM).

[0059] 6. Experimental results (1) Effect of the composition containing Xanthoceras sorbifolium Bunge oil microcapsule powder on the lifespan of AD Drosophila melanogaster Such as Figure 1As shown, the average lifespan of wild-type W1118 control group Drosophila was 39.4 d, the average lifespan of Drosophila in the AD model group (AD) was 27.7 d, the average lifespan of Drosophila in the intervention group with 5 g / kg microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil (5 g / kg AD-XSO) was 33.5 d, the average lifespan of Drosophila in the intervention group with 10 g / kg microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil (10 g / kg AD-XSO) was 36.6 d, the average lifespan of Drosophila in the intervention group with 20 g / kg microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil (20 g / kg AD-XSO) was 31.7 d, and the average lifespan of Drosophila in the intervention group with 40 g / kg microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil (40 g / kg AD-XSO) was 24 d. This indicates that among the four intervention groups with microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil, the interventions with 5 g / kg, 10 g / kg, and 20 g / kg microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil could all extend the lifespan of AD Drosophila, while the 40 g / kg microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil had a negative impact on the lifespan of Drosophila. This shows that the microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil exceeding a certain concentration will shorten the lifespan of Drosophila and accelerate the death of Drosophila, and there is a dose-effect relationship between the survival time of Drosophila and the concentration of the microencapsulated powder composition of Xanthoceras sorbifolia Bunge oil. The above results suggest that AD transgene affects the lifespan of Drosophila, and an appropriate dose of the microencapsulated powder composition of Xanthoceras sorbifolia Bunge oil can extend the lifespan of AD Drosophila. Therefore, a 10 g / kg dose of the microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil was used in subsequent experiments.

[0060] (2)Effect of microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil on olfactory memory of AD Drosophila Learning and memory, as the advanced functions of the animal body, have always been the focus of research in the nervous system. Olfactory memory is a type of learning and memory. As Figure 2 shown, with the increase of the detection time, the number of Drosophila in each group entering the unobstructed centrifuge tube showed an upward trend. Compared with the wild-type W1118 control group Drosophila, the number of Drosophila in the AD model group (AD) entering the unobstructed centrifuge tube was significantly reduced, with statistical differences (P < 0.01 or P < 0.001), indicating that AD transgenic Drosophila would cause impaired olfactory memory ability. Compared with the Drosophila in the AD model group, the number of Drosophila in the intervention group with microencapsulated powder composition of Xanthoceras sorbifolia Bunge oil (AD-XSO) entering the unobstructed centrifuge tube increased. This shows that AD will lead to a decline in the cognitive ability of Drosophila, while the intervention with the microencapsulated powder composition of Xanthoceras sorbifolia Bunge oil can improve the olfactory learning and memory ability of AD Drosophila.

[0061] (3)Effect of microencapsulated powder composition containing Xanthoceras sorbifolia Bunge oil on the expression of Aβ42 protein in the brain tissue of AD Drosophila As Figure 3As shown in the figure, Figure A shows the expression level of soluble Aβ42 protein detected by ELISA method. In the brain tissue of wild-type W1118 control group Drosophila, the content of soluble Aβ42 is very small, while in the AD model group (AD), the expression level of soluble Aβ42 protein is significantly increased (P < 0.001). Compared with the AD model group, the expression level of soluble Aβ42 protein in Drosophila of the Xanthoceras sorbifolia Bunge oil microcapsule powder composition intervention group (AD-XSO) is significantly decreased (P < 0.001). Figure B shows the expression level of insoluble Aβ42 protein detected by ELISA method. Compared with the wild-type W1118 control group Drosophila, the expression level of insoluble Aβ42 protein in the AD model group (AD) is significantly increased (P < 0.001). Compared with the AD model group, the expression level of insoluble Aβ42 protein in Drosophila of the Xanthoceras sorbifolia Bunge oil microcapsule powder composition intervention group (AD-XSO) is significantly decreased (P < 0.001). (4)Effect of the composition containing Xanthoceras sorbifolia Bunge oil microcapsule powder on the antioxidant capacity of AD Drosophila SOD and GSH-Px are important antioxidant enzymes in the body, and their activities can reflect the antioxidant capacity of the body. As Figure 4 shown, compared with the wild-type W1118 control group Drosophila, the activities of SOD and GSH-Px in the AD model group Drosophila (AD) are significantly decreased (P < 0.001), indicating that the antioxidant capacity in the body of AD Drosophila is significantly reduced; compared with AD Drosophila, the activities of SOD and GSH-Px in the body of Drosophila in the Xanthoceras sorbifolia Bunge oil microcapsule powder composition intervention group (AD-XSO) are both significantly increased (P < 0.001), indicating that AD Drosophila has an obvious oxidative stress response. Feeding with the Xanthoceras sorbifolia Bunge oil microcapsule powder composition can effectively improve the function of the antioxidant system in the brain of Drosophila, thereby reducing the damage caused by oxidative stress.

[0062] The above-described embodiments are part of the embodiments of the present invention, rather than all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the present invention claimed, but merely represents the selected embodiments of the present invention. All other embodiments obtained by relevant deductions and substitutions made by those of ordinary skill in the art under the condition of the concept of the present invention without creative labor belong to the scope of protection of the present invention.

Claims

1. A microcapsule powder composition containing Xanthoceras sorbifolia oil, characterized in that: It is composed of Xanthoceras sorbifolia oil microcapsule powder and Chinese medicine microcapsule powder; In parts by weight, the Chinese medicinal raw materials in the Chinese medicinal microcapsule powder are composed of 10-13 parts of ginseng, 10-12 parts of polygala, 10-12 parts of tuckahoe, 10 parts of poria, 5-6 parts of calamus and 5 parts of spinach seeds.

2. The microcapsule powder composition containing Xanthoceras sorbifolia oil according to claim 1, characterized in that: The particle size of the Xanthoceras sorbifolia oil microcapsule powder is 10-40 μm, and the particle size of the traditional Chinese medicine microcapsule powder is 3-11 μm.

3. The microcapsule powder composition containing Xanthoceras sorbifolia oil according to claim 1, characterized in that: Calculated by mass ratio, the ratio of the Xanthoceras sorbifolia oil microcapsule powder to the traditional Chinese medicine microcapsule powder is 4-8:

6.

4. The microcapsule powder composition containing Xanthoceras sorbifolia oil according to claim 3, characterized in that: In terms of mass ratio, the ratio of the Xanthoceras sorbifolia oil microcapsule powder to the traditional Chinese medicine microcapsule powder is 4:

6.

5. The method for preparing the microcapsule powder composition containing Xanthoceras sorbifolia oil according to any one of claims 1 to 4, characterized in that: include: The Xanthoceras sorbifolia seed kernel oil is emulsified and then embedded with a wall material to prepare the Xanthoceras sorbifolia oil microcapsule powder; The Chinese medicine raw material is extracted with alcohol and / or water and then concentrated to obtain a Chinese medicine extract. The Chinese medicine extract is mixed with water and embedded with a wall material to obtain the Chinese medicine microcapsule powder.

6. The method for preparing the microcapsule powder composition containing Xanthoceras sorbifolia oil according to claim 5, characterized in that: The emulsifier used in the emulsification is Tween 80; The wall materials used in the Xanthoceras sorbifolia oil microcapsule powder are gelatin and sucrose; The wall materials used in the traditional Chinese medicine microcapsule powder are gelatin and sucrose.

7. The method for preparing the microcapsule powder composition containing Xanthoceras sorbifolia oil according to claim 5, characterized in that: The Chinese herbal medicine extract is mixed with water in a ratio of 10-30 g:100 mL.

8. The method for preparing the microcapsule powder composition containing Xanthoceras sorbifolia oil according to claim 6, characterized in that: The mass ratio of gelatin to sucrose in the Xanthoceras sorbifolia oil microcapsule powder is 1:3-6; The mass ratio of gelatin to sucrose in the traditional Chinese medicine microcapsule powder is 5:1-3.

9. Use of the microcapsule powder composition containing Xanthoceras sorbifolia oil according to any one of claims 1 to 4 in the preparation of a drug for Alzheimer's disease.

10. The use according to claim 9, characterized in that: The microcapsule powder composition containing Xanthoceras sorbifolia oil improves at least one of lifespan, olfactory memory, brain tissue Aβ42 protein expression level, and antioxidant capacity.

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