Medicine for treating diabetes and preparation method thereof

Abstract

The invention discloses a medicine for treating diabetes and a preparation method thereof. The medicine is prepared from the following raw material components in parts by weight: 15-20 parts of folium mori, 10-20 parts of astragalus membranaceus, 8-11 parts of bamboo leaves, 9-11 parts of purslane, 5-8 parts of dendrobe, 5-10 parts of radix ophiopogonis, 3-5 parts of Chinese yam, 2-4 parts of cordyceps sinensis, 5-15 parts of radix rehmanniae recen, 5-10 parts of radices trichosanthis, 5-10 parts of radix puerariae, 2-5 parts of coptis chinensis and 2-5 parts of salvia miltiorrhiza. According to the medicine for treating diabetes mellitus provided by the invention, through the elaborately screened raw material components and the proportion thereof as well as the specific preparation method, the medicine has a remarkable curative effect and can be used for effectively treating diabetes mellitus. Meanwhile, the medicine disclosed by the invention is small in toxic and side effects, free of complications and relatively low in treatment cost, and provides a more economical and safer treatment choice for patients. In addition, the preparation method disclosed by the invention is simple, convenient and feasible, is suitable for industrial production and is beneficial to popularization and application of medicines.

Description

Technical Field

[0001] The present invention relates to the technical field of medicines for treating diabetes, in particular to a medicine for treating diabetes and a preparation method thereof. Background Art

[0002] Diabetes is an incurable chronic disease that requires lifelong medication and is one of the major diseases that endanger human health. Medical experts state that a healthy diet and lifestyle are the best remedies for alleviating diabetes.

[0003] At present, the drugs for treating diabetes are mainly Western medicine, including sulfonylureas, glucosidase inhibitors, biguanides, insulin and other drugs. However, these drugs are often accompanied by significant side effects and may cause damage to body organs. The human body is also prone to developing resistance to these drugs, resulting in the ineffectiveness of long-term use of Western medicine. In the end, patients may only be able to rely on insulin injections to control blood sugar levels, thus falling into a vicious cycle of hyperinsulinism.

[0004] In contrast, Chinese medicine is favored for its less toxic side effects, long-lasting effects, mild treatment methods and fewer complications. In addition, the wide range of Chinese medicine options makes it a research topic that has attracted much attention to give full play to the advantages of Chinese medicine, carefully select medicinal materials, and develop effective Chinese medicine for the treatment of diabetes. Summary of the invention

[0005] The object of the present invention is to provide a medicine for treating diabetes and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: a medicine for treating diabetes, which is made of the following raw material components in the following weight ratio:

[0007] 15-20 parts of mulberry leaves, 10-20 parts of astragalus, 8-11 parts of bamboo leaves, 9-11 parts of purslane, 5-8 parts of dendrobium, 5-10 parts of ophiopogon, 3-5 parts of yam, 2-4 parts of cordyceps, 5-15 parts of rehmannia, 5-10 parts of trichosanthes, 5-10 parts of kudzu root, 2-5 parts of coptis, and 2-5 parts of salvia.

[0008] Preferably, the weight ratio of the raw material components is: 20 parts of mulberry leaves, 15 parts of astragalus, 8 parts of bamboo leaves, 10 parts of purslane, 5 parts of dendrobium, 5 parts of ophiopogon, 5 parts of yam, 2 parts of cordyceps, 10 parts of rehmannia, 5 parts of trichosanthes, 8 parts of kudzu root, 2 parts of coptis, and 5 parts of salvia miltiorrhiza.

[0009] Preferably, the weight ratio of the raw material components is: 15 parts of mulberry leaves, 20 parts of astragalus, 10 parts of bamboo leaves, 9 parts of purslane, 6 parts of dendrobium, 6 parts of ophiopogon, 3 parts of yam, 3 parts of cordyceps, 13 parts of rehmannia, 5 parts of trichosanthes, 5 parts of kudzu root, 3 parts of coptis, and 2 parts of salvia miltiorrhiza.

[0010] A method for preparing a medicine for treating diabetes, comprising the following steps: first, crushing mulberry leaves, astragalus, bamboo leaves, dendrobium and ophiopogon, adding water accounting for 8 to 10 times the weight of the raw materials, heating and decocting for 2 hours at a slightly boiling state, decocting for 2 to 3 times, filtering, adding water accounting for 6 to 7 times the weight of the residue, decocting for 1.5 hours at a slightly boiling state, filtering, combining the two filtrates, concentrating under reduced pressure until the concentrated solution is 1 / 5 to 1 / 6 of the original weight, adding ethanol accounting for 2 times the total weight of the concentrated solution and having a concentration of 95%, stirring evenly, precipitating with alcohol for 48 hours, centrifuging to obtain the supernatant, concentrating under reduced pressure to remove the ethanol, obtaining a concentrated drug solution containing 1 / 20 of the weight of the alcohol-containing concentrated solution, spray drying the concentrated drug solution to obtain a dried product;

[0011] Step 2: After pulverizing purslane, yam, raw rehmannia root, trichosanthes root, kudzu root, coptis root and salvia miltiorrhiza, add 0.05-0.08 mol / L hydrochloric acid accounting for 6-10 times the weight of the above raw materials, cold soak for 2-3 hours, filter, add 0.05-0.08 mol / L hydrochloric acid accounting for 6-7 times the weight of the residue, cold soak for 1.5-2 hours, filter, combine the two filtrates, adjust the pH value to 8-10 with 0.2 mol / L sodium hydroxide, precipitate for 8-15 hours, centrifuge to obtain the precipitate, dry and grind the precipitate to obtain precipitate powder;

[0012] Step 3, grinding the cordyceps powder into fine powder of 100-250 mesh to obtain cordyceps fine powder;

[0013] Step 4: Evenly mix the obtained dried product, precipitated powder and Cordyceps sinensis powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules;

[0014] or:

[0015] Step 1: grind mulberry leaves, astragalus, bamboo leaves, purslane, dendrobium, ophiopogon, yam, cordyceps, raw rehmannia, trichosanthes, kudzu root, coptis root, and salvia miltiorrhiza, add water accounting for 8 to 10 times the weight of the above raw materials, heat and boil for 2 hours, and boil for 2 to 3 times, then filter, add water accounting for 6 to 7 times its weight to the residue, boil for 1.5 hours at a slight boiling state, filter, combine the two filtrates, and concentrate under reduced pressure until the concentrated solution is 1 / 5 to 1 / 6 of the original weight, then add 95% ethanol accounting for 2 times the total weight of the above concentrated solution, stir evenly, precipitate with alcohol for 48 hours, centrifuge to obtain the supernatant, and concentrate under reduced pressure to remove ethanol to obtain a concentrated solution containing 1 / 20 of the weight of the alcohol concentrated solution, and spray dry the concentrated solution to obtain a dry product;

[0016] Step 2: Grind the cordyceps sinensis powder into fine powder of 100-250 meshes to obtain cordyceps sinensis fine powder;

[0017] Step 3: Evenly mix the obtained dried product and the Cordyceps sinensis powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules.

[0018] Preferably, in step 4, a proper amount of lubricant can be added before granulation to improve the fluidity of the particles and facilitate tableting or capsule filling; the lubricant can be selected from one or more of magnesium stearate, talc or silicon dioxide.

[0019] Preferably, when the tablets are pressed, an appropriate amount of disintegrant can be added to promote rapid disintegration of the tablets in the body and release of the drug; the disintegrant can be selected from one or more of cross-linked sodium carboxymethyl cellulose, cross-linked polyvinylpyrrolidone or low-substituted hydroxypropyl cellulose.

[0020] Preferably, the total amount of the raw material contained in each unit dose of the prepared tablets or capsules is adjusted according to clinical needs to meet the treatment needs of different patients.

[0021] The invention can effectively treat diabetes, has the advantages of small toxic and side effects, no complications, low treatment costs, good treatment effects and simple preparation method.

[0022] In the present invention:

[0023] Mulberry leaves; contain rutin, quercetin, isoquercetin, quercetin-3-triglucoside, hemolysin, sitosterol, acetic acid, propionic acid, butyric acid, isobutyric acid, etc. in the volatile oil components, glutamic acid, aspartic acid, sucrose, glucose, fructose and multiple vitamins. It has been proven that mulberry leaf extract can treat diabetes in rabbits with fasting blood sugar and adrenaline hyperglycemia as indicators of alloxan diabetes, and has no damage to their internal organs. Mulberry leaves have the functions of dispelling wind and heat, cooling blood and improving eyesight, and stopping dryness and quenching thirst.

[0024] Bamboo leaves: enter the heart, lung, gallbladder and stomach meridians. It has the functions of clearing away heat and relieving restlessness, promoting fluid production and promoting urination, and relieving dryness and thirst.

[0025] Purslane, also known as purslane grass and purslane vegetable, has the functions of clearing away heat and detoxifying, reducing swelling and relieving pain, removing carbuncle and malignant ulcer, and relieving thirst. It contains a large amount of norepinephrine, a large amount of potassium salt, dihydroxyphenylethylamine, dihydroxyphenylalanine, glutamic acid, aspartic acid,

[0026] Sucrose, glucose, fructose and multivitamins, etc. It has been proven that purslane extract can prolong the life of some alloxan diabetic rabbits, but does not affect blood sugar levels, so it may improve the lipid metabolism disorder of animals.

[0027] Dendrobium; also known as Linlan and Jinsheng, contains dendrobium alkaloids, dendrobium amine, mucus, starch, etc. Dendrobium has the effects of promoting body fluid and benefiting the stomach, clearing away heat and nourishing yin.

[0028] Yam: contains saponin, choline, dendrobium amine, mucus, starch, glycoprotein, free amino acids, arginine, polyphenol oxidase, vitamin C, etc. Yam has the functions of strengthening the spleen, nourishing the lungs, strengthening the kidneys, and benefiting the essence. It can treat diarrhea due to spleen deficiency, cough due to consumptive disease, frequent urination, and thirst.

[0029] Cordyceps sinensis; contains crude protein, crude fiber, fatty acids, unsaturated fatty acids, cordycepic acid, isomers of quinic acid, cordycepin, free amino acids, vitamins, etc. It has a dilating effect on the trachea, can enhance the effect of adrenaline, has an inhibitory effect on the heart, has a blood pressure lowering effect, etc. It can replenish deficiency, benefit essence and qi, stop cough and resolve phlegm.

[0030] Astragalus nourishes qi and strengthens the spleen, Ophiopogon japonicus and Rehmannia root nourish yin and clear away heat, Pueraria root and Radix Trichosanthis promote fluid production and quench thirst, Coptis chinensis clears heat and dries dampness, and Salvia miltiorrhiza promotes blood circulation and removes blood stasis. All the medicines work together to achieve the effects of nourishing yin and clearing heat, nourishing qi and promoting fluid production, and promoting blood circulation and unblocking collaterals.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The medicine for treating diabetes provided by the present invention has a significant curative effect through carefully selected raw material components and their proportions, and a specific preparation method, and can effectively treat diabetes. At the same time, the medicine of the present invention has small toxic and side effects, no complications, and relatively low treatment costs, providing patients with a more economical and safe treatment option. In addition, the preparation method of the present invention is simple and easy to implement, suitable for industrial production, and is conducive to the promotion and application of the medicine. The tablets or capsules prepared by the method of the present invention, the total amount of the raw material contained in each unit dose can be adjusted according to clinical needs, so as to meet the treatment needs of different patients, and have high flexibility and applicability. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention.

[0034] The present invention provides a medicine for treating diabetes, which is prepared from the following raw material components in weight ratio:

[0035] 15-20 parts of mulberry leaves, 10-20 parts of astragalus, 8-11 parts of bamboo leaves, 9-11 parts of purslane, 5-8 parts of dendrobium, 5-10 parts of ophiopogon, 3-5 parts of yam, 2-4 parts of cordyceps, 5-15 parts of rehmannia, 5-10 parts of trichosanthes, 5-10 parts of kudzu root, 2-5 parts of coptis, and 2-5 parts of salvia.

[0036] A method for preparing a medicine for treating diabetes, comprising the following steps: first, crushing mulberry leaves, astragalus, bamboo leaves, dendrobium and ophiopogon, adding water accounting for 8 to 10 times the weight of the raw materials, heating and decocting for 2 hours at a slightly boiling state, decocting for 2 to 3 times, filtering, adding water accounting for 6 to 7 times the weight of the residue, decocting for 1.5 hours at a slightly boiling state, filtering, combining the two filtrates, concentrating under reduced pressure until the concentrated solution is 1 / 5 to 1 / 6 of the original weight, adding ethanol accounting for 2 times the total weight of the concentrated solution and having a concentration of 95%, stirring evenly, precipitating with alcohol for 48 hours, centrifuging to obtain the supernatant, concentrating under reduced pressure to remove the ethanol, obtaining a concentrated drug solution containing 1 / 20 of the weight of the alcohol-containing concentrated solution, spray drying the concentrated drug solution to obtain a dried product;

[0037] Step 2: After pulverizing purslane, yam, raw rehmannia root, trichosanthes root, kudzu root, coptis root and salvia miltiorrhiza, add 0.05-0.08 mol / L hydrochloric acid accounting for 6-10 times the weight of the above raw materials, cold soak for 2-3 hours, filter, add 0.05-0.08 mol / L hydrochloric acid accounting for 6-7 times the weight of the residue, cold soak for 1.5-2 hours, filter, combine the two filtrates, adjust the pH value to 8-10 with 0.2 mol / L sodium hydroxide, precipitate for 8-15 hours, centrifuge to obtain the precipitate, dry and grind the precipitate to obtain precipitate powder;

[0038] Step 3, grinding the cordyceps powder into fine powder of 100-250 mesh to obtain cordyceps fine powder;

[0039] Step 4: Evenly mix the obtained dried product, precipitated powder and cordyceps powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules; before granulation, an appropriate amount of lubricant can be added to improve the fluidity of the granules for tableting or capsule filling; the lubricant can be selected from one or more of magnesium stearate, talc or silicon dioxide; when pressed into tablets, an appropriate amount of disintegrant can be added to promote rapid disintegration of the tablets in the body and release of the drug; the disintegrant can be selected from one or more of cross-linked sodium carboxymethyl cellulose, cross-linked polyvinylpyrrolidone or low-substituted hydroxypropyl cellulose; the total amount of the raw material contained in each unit dose of the prepared tablets or capsules is adjusted according to clinical needs to meet the treatment needs of different patients;

[0040] or:

[0041] Step 1: grind mulberry leaves, astragalus, bamboo leaves, purslane, dendrobium, ophiopogon, yam, cordyceps, raw rehmannia, trichosanthes, kudzu root, coptis root, and salvia miltiorrhiza, add water accounting for 8 to 10 times the weight of the above raw materials, heat and boil for 2 hours, and boil for 2 to 3 times, then filter, add water accounting for 6 to 7 times its weight to the residue, boil for 1.5 hours at a slight boiling state, filter, combine the two filtrates, and concentrate under reduced pressure until the concentrated solution is 1 / 5 to 1 / 6 of the original weight, then add 95% ethanol accounting for 2 times the total weight of the above concentrated solution, stir evenly, precipitate with alcohol for 48 hours, centrifuge to obtain the supernatant, and concentrate under reduced pressure to remove ethanol to obtain a concentrated solution containing 1 / 20 of the weight of the alcohol concentrated solution, and spray dry the concentrated solution to obtain a dry product;

[0042] Step 2: Grind the cordyceps sinensis powder into fine powder of 100-250 meshes to obtain cordyceps sinensis fine powder;

[0043] Step 3: Evenly mix the obtained dried product and the Cordyceps sinensis powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules.

[0044] Embodiment 1: A method for preparing a medicine for treating diabetes, step 1, pulverize 20 parts of mulberry leaves, 15 parts of astragalus, 8 parts of bamboo leaves, 5 parts of dendrobium, and 5 parts of ophiopogon, add water accounting for 10 times the weight of the above raw materials, heat and decoct for 2 hours at a slightly boiling state, and decoct twice, then filter, add water accounting for 6 times its weight to the filter residue, decoct for 1.5 hours at a slightly boiling state, filter, combine the two filtrates, and concentrate under reduced pressure until the concentrated solution is 1 / 6 of the original weight, then add 95% ethanol accounting for 2 times the total weight of the above concentrated solution, stir evenly, precipitate with alcohol for 48 hours, centrifuge to obtain the supernatant, and concentrate under reduced pressure to remove ethanol to obtain a concentrated drug solution containing 1 / 20 of the weight of the alcohol-containing concentrated solution, and spray-dry the concentrated drug solution to obtain a dry product;

[0045] Step 2: grind 10 parts of purslane, 5 parts of yam, 10 parts of raw rehmannia, 5 parts of trichosanthes, 8 parts of kudzu root, 2 parts of coptis root and 5 parts of salvia miltiorrhiza, add 0.08 mol / L hydrochloric acid accounting for 8 times the weight of the above raw materials, soak for 3 hours, filter, add 0.08 mol / L hydrochloric acid accounting for 7 times its weight to the residue, soak for 2 hours, filter, combine the two filtrates, adjust the pH to 9 with 0.2 mol / L sodium hydroxide, precipitate for 10 hours, centrifuge to obtain the precipitate, dry and grind the precipitate to obtain precipitate powder;

[0046] Step 3, grind 2 parts of Cordyceps sinensis into 200 mesh fine powder to obtain Cordyceps sinensis fine powder;

[0047] Step 4: Evenly mix the obtained dried product, precipitated powder and cordyceps powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules; before granulation, an appropriate amount of lubricant can be added to improve the fluidity of the particles for tableting or capsule filling; the lubricant can be selected from one or more of magnesium stearate, talc or silicon dioxide; when pressed into tablets, an appropriate amount of disintegrant can be added to promote rapid disintegration of the tablets in the body and release the drug; the disintegrant can be selected from one or more of cross-linked sodium carboxymethyl cellulose, cross-linked polyvinylpyrrolidone or low-substituted hydroxypropyl cellulose; the total amount of the raw material contained in each unit dose of the prepared tablets or capsules is adjusted according to clinical needs to meet the treatment needs of different patients. Embodiment 2: A method for preparing a medicine for treating diabetes, step 1, pulverize 15 parts of mulberry leaves, 20 parts of astragalus, 10 parts of bamboo leaves, 6 parts of dendrobium, and 6 parts of ophiopogon, add water accounting for 10 times the weight of the above raw materials, heat and decoct for 2 hours at a slightly boiling state, and decoct twice, then filter, add water accounting for 6 times its weight to the residue, decoct for 1.5 hours at a slightly boiling state, filter, combine the two filtrates, and concentrate under reduced pressure until the concentrated solution is 1 / 6 of the original weight, then add 95% ethanol accounting for 2 times the total weight of the above concentrated solution, stir evenly, precipitate with alcohol for 48 hours, centrifuge to obtain the supernatant, and concentrate under reduced pressure to remove ethanol to obtain a concentrated drug solution containing 1 / 20 of the weight of the alcohol-containing concentrated solution, and spray dry the concentrated drug solution to obtain a dry product;

[0048] Step 2: grind 9 parts of purslane, 3 parts of yam, 13 parts of raw rehmannia, 5 parts of trichosanthes, 5 parts of kudzu root, 3 parts of coptis, and 2 parts of salvia miltiorrhiza, add 0.08 mol / L hydrochloric acid accounting for 8 times the weight of the above-mentioned raw materials, soak for 3 hours, filter, add 0.08 mol / L hydrochloric acid accounting for 7 times its weight to the residue, soak for 2 hours, filter, combine the two filtrates, adjust the pH value to 9 with 0.2 mol / L sodium hydroxide, precipitate for 10 hours, centrifuge to obtain the precipitate, dry and grind the precipitate to obtain precipitate powder;

[0049] Step 3: Grind 3 parts of Cordyceps sinensis into 200-mesh fine powder to obtain Cordyceps sinensis fine powder;

[0050] Step 4: Evenly mix the obtained dried product, precipitated powder and cordyceps powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules; before granulation, an appropriate amount of lubricant can be added to improve the fluidity of the particles for tableting or capsule filling; the lubricant can be selected from one or more of magnesium stearate, talc or silicon dioxide; when pressed into tablets, an appropriate amount of disintegrant can be added to promote rapid disintegration of the tablets in the body and release the drug; the disintegrant can be selected from one or more of cross-linked sodium carboxymethyl cellulose, cross-linked polyvinylpyrrolidone or low-substituted hydroxypropyl cellulose; the total amount of the raw material contained in each unit dose of the prepared tablets or capsules is adjusted according to clinical needs to meet the treatment needs of different patients.

[0051] Embodiment 3: A method for preparing a medicine for treating diabetes, step 1, 20 parts of mulberry leaves, 15 parts of astragalus, 8 parts of bamboo leaves, 10 parts of purslane, 5 parts of dendrobium, 5 parts of ophiopogon, 5 parts of yam, 2 parts of cordyceps, 10 parts of raw rehmannia, 5 parts of trichosanthes, 8 parts of kudzu root, 2 parts of coptis, 5 parts of salvia miltiorrhiza, add water accounting for 10 times the weight of the above raw materials, heat and boil for 2 hours, and boil twice, then filter, add water accounting for 6 times its weight to the residue, boil for 1.5 hours under a slightly boiling state, filter, combine the two filtrates, and concentrate under reduced pressure until the concentrate is 1 / 5 of the original weight, then add 95% ethanol accounting for 2 times the total weight of the above concentrate, stir evenly, precipitate for 48 hours, centrifuge to take the supernatant, and concentrate under reduced pressure to remove ethanol to obtain a concentrated drug solution containing 1 / 20 of the weight of the alcohol concentrate, spray dry the concentrated drug solution to obtain a dry product;

[0052] Step 2: Grind 2 parts of Cordyceps sinensis into 150 mesh fine powder to obtain Cordyceps sinensis fine powder;

[0053] Step 3: Evenly mix the obtained dried product and the Cordyceps sinensis powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules.

[0054] The drug of the present invention (Example 1 is the best) has been shown in animal experiments:

[0055] 1. The drug was administered to test mice at a dose of 350 mg / kg, 250 mg / kg, and 150 mg / kg for 10 consecutive days, which significantly reduced the blood sugar level of normal test mice (p < 0.04) and increased the glycogen level (p < 0.04). Specifically, after 10 consecutive days of drug administration, the blood sugar level of the test mice was significantly controlled, and the effect was statistically significant. In addition, the drug can also promote the synthesis of glycogen, which has a positive significance for maintaining blood sugar stability. The above dose was administered to alloxan-induced hyperdiabetic test mice (the average blood sugar level was between 30 and 35) continuously. At 20 days, the average blood sugar level of the test mice was 13 to 15, and at 30 days, the average blood sugar level of the test mice was 8 to 10. This result shows that the drug can continuously and effectively control the blood sugar level of diabetic test mice during long-term use. Pathological sections showed that the drug can significantly improve the damage to the pancreas caused by modeling factors and significantly reduce the level of glycosylated serum protein (p < 0.04). This shows that the drug not only has a significant effect in blood sugar control, but also shows a positive effect in improving pancreatic tissue damage.

[0056] 2. The drug was administered to streptozotocin-induced hyperglycemia model rats at a dose of 150 mg / kg, 100 mg / kg, and 50 mg / kg for 30 consecutive days. The blood glucose level of the model rats was significantly reduced at 20 days and 30 days (p < 0.01), while the hypoglycemic effect of Diamecon and metformin was lower than that of the drug; pathological sections showed that the drug could significantly improve the damage of the pancreas caused by modeling factors, and the endocrine granules, mitochondria, and endoplasmic reticulum were more abundant than those in the model group, and the metabolism of pancreatic islet cells was active. The level of glycosylated serum protein was significantly reduced (p < 0.04), the insulin sensitivity index was significantly improved (p < 0.04), and the MDA level, TG and TDL-C (p < 0.01) in the serum of the model rats were significantly reduced. These data show that the drug can not only effectively reduce blood glucose in long-term use, but also improve the function of pancreatic islet cells, improve insulin sensitivity, reduce oxidative stress, and reduce blood lipid levels, thereby combating the development of diabetes at multiple levels.

[0057] 3. The in vitro inhibitory effect of the drug on α-glucosidase activity. Take 7 ml of 60 mmol / L potassium phosphate buffer (pH 6.7), 0.2 ml of 4 mmol / L glutathione solution, and 0.4 ml of 0.03 mg / ml α-D-glucosidase solution, incubate at 37°C for 10 min, then add 0.6 ml of 0.01 mol / L glucopyranoside solution. After 20 min, add 0.1 mol / L Na 2 CO 3 The reaction was terminated with 10 ml of the solution, and the absorbance of each group of samples was measured at a wavelength of 400 nm. Test solutions of the drug with different concentrations were prepared with potassium chloride buffer.

[0058] Inhibition rate (%) = (A vs. mouse-A sample) / (A vs. mouse-A blank) × 100%

[0059] The experiment showed that 150mg / ml of the drug can reduce the activity of α-glucosidase by 83.42%. This experimental result shows that the drug has a significant inhibitory effect on α-glucosidase in vitro, can effectively slow down the decomposition of sugar substances, and thus slow down the rise of blood sugar, which is a very promising treatment direction for diabetic patients.

[0060] Furthermore, the medicine of the present invention has a significant clinical effect in treating diabetes. Through comparative experiments, we found that the medicine can not only significantly reduce the blood sugar level of patients, but also effectively improve pancreatic islet function and promote the secretion and utilization of insulin. Compared with traditional hypoglycemic drugs, the medicine of the present invention has lower side effects and higher safety, can stably control the blood sugar level of patients for a long time, and reduce the occurrence of complications. In addition, the preparation method of the medicine is simple and easy, suitable for large-scale industrial production, and provides a more reliable and effective treatment option for diabetic patients.

[0061] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A medicine for treating diabetes, characterized in that: It is made of the following raw material components in weight ratio: 15-20 parts of mulberry leaves, 10-20 parts of astragalus, 8-11 parts of bamboo leaves, 9-11 parts of purslane, 5-8 parts of dendrobium, 5-10 parts of ophiopogon, 3-5 parts of yam, 2-4 parts of cordyceps, 5-15 parts of rehmannia, 5-10 parts of trichosanthes, 5-10 parts of kudzu root, 2-5 parts of coptis, and 2-5 parts of salvia.

2. A medicine for treating diabetes according to claim 1, characterized in that: The weight ratio of the raw material components is: 20 parts of mulberry leaves, 15 parts of astragalus, 8 parts of bamboo leaves, 10 parts of purslane, 5 parts of dendrobium, 5 parts of ophiopogon, 5 parts of yam, 2 parts of cordyceps, 10 parts of rehmannia, 5 parts of trichosanthes, 8 parts of kudzu root, 2 parts of coptis, and 5 parts of salvia miltiorrhiza.

3. A medicine for treating diabetes according to claim 1, characterized in that: The weight ratio of each raw material component is: 15 parts of mulberry leaves, 20 parts of astragalus, 10 parts of bamboo leaves, 9 parts of purslane, 6 parts of dendrobium, 6 parts of ophiopogon, 3 parts of yam, 3 parts of cordyceps, 13 parts of rehmannia, 5 parts of trichosanthes, 5 parts of kudzu root, 3 parts of coptis, and 2 parts of salvia miltiorrhiza.

4. A method for preparing a drug for treating diabetes according to any one of claims 1 to 3, characterized in that: Step 1: grind mulberry leaves, astragalus, bamboo leaves, dendrobium and ophiopogon, add water accounting for 8 to 10 times the weight of the above raw materials, heat and boil for 2 hours, and boil for 2 to 3 times, then filter, add water accounting for 6 to 7 times its weight to the residue, boil for 1.5 hours at a slight boiling state, filter, combine the two filtrates, and concentrate under reduced pressure until the concentrated solution is 1 / 5 to 1 / 6 of the original weight, then add 95% ethanol accounting for 2 times the total weight of the above concentrated solution, stir evenly, precipitate with alcohol for 48 hours, centrifuge to obtain the supernatant, concentrate under reduced pressure to remove ethanol, and obtain a concentrated solution containing 1 / 20 of the weight of the alcohol-containing concentrated solution, and spray dry the concentrated solution to obtain a dry product; Step 2: After pulverizing purslane, yam, raw rehmannia root, trichosanthes root, kudzu root, coptis root and salvia miltiorrhiza, add 0.05-0.08 mol / L hydrochloric acid accounting for 6-10 times the weight of the above raw materials, cold soak for 2-3 hours, filter, add 0.05-0.08 mol / L hydrochloric acid accounting for 6-7 times the weight of the residue, cold soak for 1.5-2 hours, filter, combine the two filtrates, adjust the pH value to 8-10 with 0.2 mol / L sodium hydroxide, precipitate for 8-15 hours, centrifuge to obtain the precipitate, dry and grind the precipitate to obtain precipitate powder; Step 3, grinding the cordyceps powder into fine powder of 100-250 mesh to obtain cordyceps fine powder; Step 4: Evenly mix the obtained dried product, precipitated powder and Cordyceps sinensis powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules; or: Step 1: grind mulberry leaves, astragalus, bamboo leaves, purslane, dendrobium, ophiopogon, yam, cordyceps, raw rehmannia, trichosanthes, kudzu root, coptis root, and salvia miltiorrhiza, add water accounting for 8 to 10 times the weight of the above raw materials, heat and boil for 2 hours, and boil for 2 to 3 times, then filter, add water accounting for 6 to 7 times its weight to the residue, boil for 1.5 hours at a slight boiling state, filter, combine the two filtrates, and concentrate under reduced pressure until the concentrated solution is 1 / 5 to 1 / 6 of the original weight, then add 95% ethanol accounting for 2 times the total weight of the above concentrated solution, stir evenly, precipitate with alcohol for 48 hours, centrifuge to obtain the supernatant, and concentrate under reduced pressure to remove ethanol to obtain a concentrated solution containing 1 / 20 of the weight of the alcohol concentrated solution, and spray dry the concentrated solution to obtain a dry product; Step 2: Grind the cordyceps sinensis powder into fine powder of 100-250 meshes to obtain cordyceps sinensis fine powder; Step 3: Evenly mix the obtained dried product and the Cordyceps sinensis powder, mix with appropriate amount of microcrystalline cellulose and sodium carboxymethyl starch, granulate, press into tablets or put into capsules.

5. A method for preparing a drug for treating diabetes according to claim 4, characterized in that: In step 4, a proper amount of lubricant can be added before granulation to improve the fluidity of the granules and facilitate tableting or capsule filling; the lubricant can be selected from one or more of magnesium stearate, talc or silicon dioxide.

6. A method for preparing a drug for treating diabetes according to claim 4, characterized in that: When compressed into tablets, an appropriate amount of disintegrant can be added to promote rapid disintegration of the tablets in the body and release of the drug; the disintegrant can be selected from one or more of cross-linked sodium carboxymethyl cellulose, cross-linked polyvinylpyrrolidone or low-substituted hydroxypropyl cellulose.

7. A method for preparing a drug for treating diabetes according to any one of claims 4 to 6, characterized in that: The total amount of the raw material contained in each unit dose of the prepared tablets or capsules is adjusted according to clinical needs to meet the treatment needs of different patients.