Comprehensive treatment method for water-soaked manure and application of product of comprehensive treatment method

Abstract

The invention relates to the technical field of organic fertilizers, and discloses a comprehensive treatment method for blister manure and application of a product thereof, and the method is characterized in that the method can treat blister manure in a large scale, different components of the blister manure are converted into available resources, and waste is turned into wealth. Specifically, after system treatment, the water-soaked manure can be converted into biogas, clear liquid and organic fertilizer, and the biogas can be collected through a pipeline and utilized, for example, for heating; clear liquid can be directly returned to the field for utilization; the organic fertilizer can be used for tomato planting. The organic fertilizer disclosed by the invention has an obvious promoting effect on plant height, stem diameter and fruits of tomatoes. The disease resistance of tomatoes to bacterial wilt and fusarium wilt can be improved, and the improvement effect is obviously better than that of the prior art.

Description

Technical Field

[0001] The present invention relates to the technical field of manure treatment, and more specifically, to a comprehensive treatment method for flush manure and the application of its products. Background Art

[0002] The flush manure technology is a manure treatment technology widely used in pig farms. By soaking pig manure and urine in water for a long time, it is decomposed by putrefactive microorganisms. This technology reduces the frequency of manure cleaning, facilitates storage, and also reduces labor intensity. However, the flush manure technology also has some problems and challenges. For example, the manure pollutants produced by the flush manure cleaning mode have a high concentration. At present, the three single disposal methods of directly returning to the field after simple dry-wet separation, integrated manure fermentation disposal (fermentation bed mode), and reaching the standard discharge mode after anaerobic treatment of flush manure cannot achieve the reasonable disposal of flush manure. Especially for large-scale farms, due to the higher amount of manure produced, the problem is particularly serious. Summary of the Invention

[0003] Aiming at the deficiencies of the existing technology, one of the purposes of the present invention is to provide a comprehensive treatment method for manure and the application of its products, which can more completely and reasonably dispose of flush manure.

[0004] To achieve the above purpose, the present invention provides the following technical solutions:

[0005] A comprehensive treatment method for flush manure, comprising the following steps:

[0006] Introduce the flush manure into a biogas digester for anaerobic biological fermentation to produce biogas and biogas slurry;

[0007] Separate the biogas slurry through a dry-wet separator to obtain upper clear liquid and bottom biogas residue;

[0008] Mix the bottom biogas residue with bedding and fermentation bacteria and stir to obtain compost, and continuously ferment the compost in a fermentation bed to obtain organic fertilizer;

[0009] Add fermentation bacteria and algae to the upper clear liquid, and after industrial AO treatment, discharge it up to the standard.

[0010] Preferably, the bedding includes straw and / or tail vegetables.

[0011] Preferably, the fermentation bacteria is Bacillus subtilis B1, with the preservation number CCTCC NO: M20241564, the preserved strain name Bacillus subtilis B1, the preservation unit China Center for Type Culture Collection, the preservation time July 15, 2024, and the preservation location Wuhan University.

[0012] Preferably, the fermented bacteria and algae are Bacillus subtilis B1 and Chlorella vulgaris CQ-1. The preservation number of Bacillus subtilis B1 is CCTCC NO: M20241564, the preserved strain name is Bacillus subtilis B1, the preservation unit is the China Center for Type Culture Collection, the preservation time is July 15, 2024, and the preservation location is Wuhan University. The preservation number of Chlorella vulgaris CQ-1 is CCTCC NO: M20231453, the preserved strain name is Chlorella vulgaris CQ-1, the preservation unit is the China Center for Type Culture Collection, the preservation time is August 11, 2023, and the preservation location is Wuhan University.

[0013] Preferably, the industrial AO is specifically the A-O-A-O mode.

[0014] The second object of the present invention is to provide an application of the product obtained by a comprehensive treatment method for water-immersed manure.

[0015] To achieve the above object, the present invention provides the following technical solution: The organic fertilizer obtained according to the above method is used for tomato planting.

[0016] The beneficial effects of the present invention: The method of the present invention can batch-treat water-immersed manure, convert different components of water-immersed manure into utilizable resources, and turn waste into treasure. Specifically, through the treatment of the system, water-immersed manure can be converted into biogas, clear liquid, and organic fertilizer. The biogas can be collected through pipelines and utilized, such as for heating; the clear liquid can be directly returned to the field for utilization; the organic fertilizer can be used for tomato planting. The organic fertilizer of the present invention has obvious promoting effects on the plant height, stem diameter, and fruits of tomatoes. It can improve the disease resistance of tomatoes to bacterial wilt and fusarium wilt, and the improvement effect is significantly better than that of the prior art. Detailed Embodiments

[0017] It should be noted that unless otherwise specified, all technical and scientific terms used in this application have the same meaning as commonly understood by those of ordinary skill in the technical field to which this application belongs. The terms used in the description of this application are only for the purpose of describing specific embodiments and are not intended to limit this application. It should be noted that, without conflict, the embodiments in this application and the features in the embodiments can be combined with each other.

[0018] To make the object, technical solution, and advantages of the present invention clearer, the following specific embodiments are used to further elaborate on the present invention in detail. It should be noted that the specific embodiments below are only for illustration and do not limit the present invention.

[0019] Tomato bacterial wilt is a disease caused by Pseudomonas solanacearum, posing a serious threat to tomato crops.

[0020] Symptom manifestations: Leaves: At the initial stage, the new leaves at the top wilt and droop, and then the lower leaves develop wilting, followed by the middle leaves. After the onset of the disease, the leaf color is lighter, showing a bacterial wilt state. The leaves wilt during the day and return to normal after evening, but soon spread to the whole plant and wilt, and no longer recover and die.

[0021] Stem: Initially, it is a water-soaked spot, and after expansion, it becomes a brown patch of 1-2 cm. The epidermis of the middle and lower parts of the diseased stem is rough, and adventitious roots often appear. When the diseased stem is cut open, the vascular bundles of the diseased stem turn brown, and when cut transversely and squeezed by hand, milky white mucus can be seen oozing out, which is a typical symptom of bacterial wilt.

[0022] Tomato fusarium wilt is a soil-borne disease caused by Fusarium oxysporum f.sp.lycopersici, causing serious damage to tomato crops and leading to a significant decline in yield and quality. The course of this disease progresses slowly, usually taking 15 to 30 days to kill the plant, and the vascular bundles of the diseased plant turn brown. Under high humidity conditions, a pink mold layer may appear on the diseased part.

[0023] Example 1:

[0024] A comprehensive treatment method for flooded manure includes the following steps:

[0025] S1, Introduce the flooded manure into the biogas digester for anaerobic biological fermentation to produce biogas and biogas slurry.

[0026] Specifically, introduce the flooded manure generated from pig breeding into the biogas digester, and at the same time add the existing biogas residue or inoculum containing a large amount of microorganisms to initiate the fermentation process. Seal the biogas digester to ensure an anoxic environment is formed inside for anaerobic fermentation under anoxic conditions. As the fermentation process progresses, biogas and biogas slurry are produced, and the biogas can be collected through pipelines and utilized, such as for heating.

[0027] S2, Separate the biogas slurry through a solid-liquid separator to obtain the upper clear liquid and the bottom biogas residue.

[0028] S3, Mix and stir the bottom biogas residue with rape straw and fermentation bacteria to obtain compost, and the compost is continuously fermented in the fermentation bed to obtain organic fertilizer. The fermentation bacteria is Bacillus subtilis B1, with the preservation number CCTCC NO:M20241564, the preserved strain name Bacillus subtilis B1, the preservation unit China Center for Type Culture Collection, the preservation time July 15, 2024, and the preservation location Wuhan University.

[0029] S4. After adding fermentative bacteria and algae to the upper clear liquid, it is treated by industrial AO and discharged up to the standard. The fermentative bacteria and algae are Bacillus subtilis B1 and Chlorella vulgaris CQ-1. The preservation number of Bacillus subtilis B1 is CCTCC NO: M20241564, the preserved strain name is Bacillus subtilis B1, the preservation unit is China Center for Type Culture Collection, the preservation time is July 15, 2024, and the preservation location is Wuhan University. The preservation number of Chlorella vulgaris CQ-1 is CCTCC NO: M20231453, the preserved strain name is Chlorella vulgaris CQ-1, the preservation unit is China Center for Type Culture Collection, the preservation time is August 11, 2023, and the preservation location is Wuhan University.

[0030] Example 2:

[0031] A comprehensive treatment method for flushing manure includes the following steps:

[0032] S1. Introduce the flushing manure into the biogas digester for anaerobic biological fermentation to produce biogas and biogas slurry.

[0033] Specifically, introduce the flushing manure generated from pig breeding into the biogas digester, and at the same time add the existing biogas residue or inoculum containing a large amount of microorganisms to initiate the fermentation process. Seal the biogas digester to ensure an anoxic environment is formed inside for anaerobic fermentation under anoxic conditions. As the fermentation process progresses, biogas and biogas slurry are produced. The biogas can be collected through pipelines and utilized, such as for heating.

[0034] S2. Separate the biogas slurry through a solid-liquid separator to obtain the upper clear liquid and the bottom biogas residue.

[0035] Specifically, pump the fermented biogas slurry into the solid-liquid separator. The separation process includes:

[0036] Extrusion: The solid-liquid separator separates the solid substances from the liquid in the biogas slurry by means of screw extrusion.

[0037] Pressing: Apply pressure through a pressing device to further squeeze out the moisture in the solid substances and improve the dryness of the solid substances.

[0038] Precipitation: Let the solid particles precipitate in the precipitation area of the solid-liquid separator under the action of gravity to make the liquid clearer.

[0039] S3. Mix the bottom biogas residue with rice straw and fermentation bacteria and stir to obtain compost, and continuously ferment the compost in the fermentation bed to obtain organic fertilizer. The fermentation bacteria is Bacillus subtilis B1, with the preservation number CCTCC NO: M20241564, the preserved strain name Bacillus subtilis B1, the preservation unit China Center for Type Culture Collection, the preservation time July 15, 2024, and the preservation location Wuhan University.

[0040] S4. After adding fermentation bacteria and algae to the upper clear liquid, it is treated by A-O-A—O and then discharged up to standard. The fermentation bacteria and algae are Bacillus subtilis B1 and Chlorella vulgaris CQ-1. The preservation number of Bacillus subtilis B1 is CCTCC NO: M20241564, the preserved strain name Bacillus subtilis B1, the preservation unit China Center for Type Culture Collection, the preservation time July 15, 2024, and the preservation location Wuhan University. The preservation number of Chlorella vulgaris CQ-1 is CCTCC NO: M20231453, the preserved strain name Chlorella vulgaris CQ-1, and the preservation unit is China Center for Type Culture Collection for both, the preservation time August 11, 2023, and the preservation location Wuhan University.

[0041] The A-O-A—O process is mainly divided into four functional sections: anaerobic section, aerobic section, anoxic section, and re-aerobic section. The functions of each section are as follows:

[0042] Anaerobic Zone: Release phosphorus. Under anaerobic conditions, polyphosphate-accumulating organisms release phosphorus in the cells, increasing the phosphorus concentration in the sewage, and at the same time absorbing dissolved organic matter in the sewage, decreasing the BOD concentration. Dissolved oxygen concentration: generally less than 0.2 mg / L.

[0043] Oxic Zone: Degrade organic matter, nitrify ammonia nitrogen, and absorb excessive phosphorus. Under aerobic conditions, aerobic microorganisms oxidize and decompose organic matter in the sewage, and at the same time oxidize ammonia nitrogen into nitrate nitrogen, and polyphosphate-accumulating organisms absorb more phosphorus. Dissolved oxygen concentration: generally not less than 2 mg / L.

[0044] Anoxic Zone: Denitrify and remove nitrogen. Under anoxic conditions, denitrifying bacteria use organic matter in the sewage as a carbon source to reduce nitrate and nitrite in the reflux mixture to nitrogen gas, which escapes into the atmosphere, thus achieving nitrogen removal. Dissolved oxygen concentration: generally 0.2 - 0.5 mg / L.

[0045] Re-aerobic Section: Further degrade organic matter and nitrify ammonia nitrogen to ensure that the effluent quality meets the standard. Through re-aeration, further remove residual organic matter and ammonia nitrogen to improve the effluent quality. Dissolved oxygen concentration: generally not less than 2 mg / L.

[0046] Example 3:

[0047] The difference from Example 1 is that the bedding material is waste broccoli stems and leaves.

[0048] Example 4:

[0049] The difference from Example 1 is that the bedding material is waste green vegetable stems and leaves.

[0050] Comparative Example 1:

[0051] The difference from Example 1 is that the fermentation bacteria are commercially available Bacillus subtilis, purchased from Yaobo Biotechnology (Jinan) Co., Ltd.

[0052] Comparative Example 2:

[0053] The difference from Example 1 is that the fermentation bacteria are commercially available Bacillus subtilis, purchased from Beihai Qiangxing Biotechnology Co., Ltd.

[0054] The tomato variety selected is the Gray tomato from Shandong Lingguang Agricultural Technology Co., Ltd. 400 Gray tomatoes were randomly and evenly divided into 4 groups. The organic fertilizers obtained in Example 1, Example 2, Comparative Example 1 and Comparative Example 2 were respectively applied as base fertilizers near the roots of the above 4 groups of Gray tomatoes. The base fertilizer application rate was 5000 kg / hm 2 , and compound fertilizer was applied. The contents of N, P, and K in the compound fertilizer were all 15%, and the compound fertilizer application rate was 500 kg / hm 2 . When planting, the distance between adjacent plants was 60 cm, and other management was carried out according to the routine. After the seedlings were established and the slow seedling stage passed, the plant height and stem diameter of the tomatoes were investigated every 20 days. At the tomato fruit maturity stage, tomato fruits were collected, and the single fruit weight and single plant yield of the tomatoes were measured. The specific statistical data results are shown in Table 1.

[0055] Table 1: Effects of different organic fertilizers on tomato plant height, stem diameter, single fruit weight and single plant yield

[0056]

[0057] As can be seen from Table 1, the plant height and stem diameter of the tomatoes planted with the organic fertilizers of Example 1 and 2, as well as the single fruit weight and single plant weight of the tomato fruits, were significantly higher than those of the tomatoes planted with the organic fertilizers of Comparative Example 1 and 2 at each planting stage. Thus, it can be seen that the Bacillus subtilis B1 and its fermentation products of the present invention have obvious promoting effects on the plant height, stem diameter and fruits of tomatoes, and the promoting effect is significantly better than that of commercially available Bacillus subtilis and its fermentation products.

[0058] The tomato variety selected was the Gray tomato from Shandong Lingguang Agricultural Technology Co., Ltd. 400 Gray tomatoes were randomly and evenly divided into 4 groups, and the organic fertilizers obtained in Example 1, Example 2, Comparative Example 1, and Comparative Example 2 were applied as base fertilizers near the roots of the above 4 groups of Gray tomatoes, with the base fertilizer application rate being 5000 kg / hm 2 , and compound fertilizer was applied. The contents of N, P, and K in the compound fertilizer were all 15%, and the application rate of the compound fertilizer was 500 kg / hm 2 . When planting, the distance between adjacent plants was 60 cm, and other management was carried out according to the routine. After the tomatoes fruited, 2 mL of 0.1% Pseudomonas solanacearum bacterial solution was sprayed on each tomato plant, and the number of diseased plants was recorded every 2 days. The specific statistical data results are shown in Table 2.

[0059] Table 2: Effects of different organic fertilizers on the disease resistance of tomatoes to bacterial wilt

[0060]

[0061] As can be seen from Table 2, the number of tomato plants suffering from bacterial wilt after applying the organic fertilizers prepared in Comparative Examples 1 and 2 was significantly higher than that of the tomatoes after applying the organic fertilizers prepared in Examples 1 and 2. Thus, it can be seen that the Bacillus subtilis B1 and its fermentation product of the present invention can significantly improve the disease resistance of tomatoes to bacterial wilt. And the improvement effect of enhancing the disease resistance of tomatoes to bacterial wilt is significantly better than that of commercially available Bacillus subtilis and its fermentation products.

[0062] The tomato variety selected was the Gray tomato from Shandong Lingguang Agricultural Technology Co., Ltd. 400 Gray tomatoes were randomly and evenly divided into 4 groups, and the organic fertilizers obtained in Example 1, Example 2, Comparative Example 1, and Comparative Example 2 were applied as base fertilizers near the roots of the above 4 groups of Gray tomatoes, with the base fertilizer application rate being 5000 kg / hm 2 , and compound fertilizer was applied. The contents of N, P, and K in the compound fertilizer were all 15%, and the application rate of the compound fertilizer was 500 kg / hm 2 . When planting, the distance between adjacent plants was 60 cm, and other management was carried out according to the routine. After the tomatoes blossomed, 2 mL of 0.1% Fusarium oxysporum bacterial solution was sprayed on the roots of each tomato plant, and the number of diseased plants was recorded every 5 days. The specific statistical data results are shown in Table 3.

[0063] Table 3: Effects of different organic fertilizers on the disease resistance of tomatoes to fusarium wilt

[0064]

[0065] As can be seen from Table 3, the number of tomato plants suffering from fusarium wilt after applying the organic fertilizers prepared in Comparative Examples 1 and 2 is significantly higher than that of the tomatoes applying the organic fertilizers prepared in Examples 1 and 2. Thus, it can be seen that the Bacillus subtilis B1 and its fermented product of the present invention can significantly improve the disease resistance of tomatoes to fusarium wilt. And the improvement effect of improving the disease resistance of tomatoes to fusarium wilt is significantly better than that of the commercially available Bacillus subtilis and its fermented product.

[0066] The above description is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions falling within the idea of the present invention belong to the protection scope of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A comprehensive treatment method for blistered feces, characterized in that: The following steps are involved: The water-soaked feces are introduced into the biogas tank for anaerobic biological fermentation to produce biogas and biogas liquid; The biogas slurry is separated by a dry-wet separator to obtain an upper clear liquid and a bottom biogas residue; The bottom biogas residue is mixed with bedding and fermentation bacteria to obtain compost, and the compost is continuously fermented in a fermentation bed to obtain organic fertilizer; The supernatant is added with fermented algae and then treated with industrial AO before being discharged in compliance with the discharge standards.

2. A method for comprehensive treatment of foamy feces according to claim 1, characterized in that: The bedding material includes straw and / or vegetable tail.

3. The method for comprehensive treatment of foamy feces according to claim 1, characterized in that: The fermentation bacteria is Bacillus subtilis B1, the preservation number is CCTCC NO: M20241564, and the preservation unit is China Center for Type Culture Collection.

4. The method for comprehensive treatment of foamy feces according to claim 1, characterized in that: The fermented bacteria and algae are Bacillus subtilis B1 and Chlorella CQ-1, the preservation number of the Bacillus subtilis B1 is CCTCC NO: M20241564, and the preservation number of the Chlorella CQ-1 is CCTCC NO: M20231453, and the preservation units are both China Center for Type Culture Collection.

5. The method for comprehensive treatment of foamy feces according to claim 1, characterized in that: The industrialized AO is specifically the AOAO model.

6. The organic fertilizer obtained by the comprehensive treatment method of water-soaked manure according to any one of claims 1 to 5 is used for tomato planting.

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