Composition for preventing and treating areca tree yellows and preparation method thereof

Abstract

The invention relates to the technical field of areca tree planting, in particular to a composition for preventing and treating areca tree yellows and a preparation method thereof. The composition comprises a first mixed fertilizer, a second mixed fertilizer and a third mixed fertilizer. According to the invention, the organic fertilizer is combined with the mixed bacteria and the mixed enzyme, the mixed bacteria secrete metabolites to improve the granular structure of soil, and the mixed enzyme decomposes organic matters, releases nitrogen, phosphorus, potassium and medium trace elements and activates soil fixed-state phosphorus, so that nutrient imbalance caused by degeneration of root systems of areca-nuts is relieved; meanwhile, trichoderma in the mixed bacteria directly parasitizes or competitively inhibits soil-borne pathogens, a probiotic barrier is constructed through cooperation of multiple strains, the infection risk of etiology of the yellows is reduced, bacillus licheniformis can generate growth-promoting hormones and stimulate regeneration of capillary roots, EM flora metabolites improve the root activity, SOD enzyme scavenges free radicals, and the growth-promoting effect is achieved. Plasmin improves the permeability of xylem conduits, promotes moisture and nutrient transportation, relieves leaf yellowing, and achieves the fundamental prevention and control of areca catechu yellows.

Description

Technical Field

[0001] The invention relates to the technical field of betel nut planting, in particular to a composition for preventing and treating betel nut yellowing disease and a preparation method thereof. Background Art

[0002] Areca palm is a shallow-rooted tree species belonging to the order Primitiformes, family Areca, class Magnoliaceae. The root system is fibrous, without obvious taproots. At 3 years old, it is a young tree with about 120 lateral roots. At 10 years old, it is a middle-aged tree with about 180 lateral roots. At 30 years old, it is a mature tree with about 400 lateral roots. At 50 years old, it is an old tree with the root system reduced to about 100 lateral roots. The width of the lateral roots is about 90 cm. The depth of the roots in the soil is about 160 cm. There are also some short and thick aerial roots around the trunk of the Areca palm.

[0003] In the prior art, due to the long-term lack of fertilizers at the nutritional level, especially fertilizers of trace elements and rare elements, and the long-term application of chemical fertilizers and lack of organic fertilizers, the soil has become compacted and eroded, and the aging of the root system has caused the nutrient absorption function to decline, leading to malnutrition. At the same time, the tree is invaded by bacterial, fungal and viral diseases, which makes it easy for the betel nut tree to develop chlorosis when planted.

[0004] Based on this, the present invention provides a composition for preventing and treating areca catechu yellowing disease and a preparation method thereof. Summary of the invention

[0005] The object of the present invention is to provide a composition for preventing and treating the chlorosis of betel nut trees and a preparation method thereof. The composition for preventing and treating the chlorosis of betel nut trees prepared by the present invention can effectively prevent and treat the chlorosis phenomenon of betel nut trees and promote the normal growth of betel nut trees.

[0006] To achieve the above object, the present invention provides the following technical solutions: a composition for preventing and treating chlorosis of betel nut trees and a preparation method thereof, comprising a first mixed fertilizer, a second mixed fertilizer and a third mixed fertilizer;

[0007] The first mixed fertilizer is composed of the following raw materials in parts by weight: 85-90 parts of organic fertilizer, 1-3 parts of Bacillus subtilis, 1-3 parts of Trichoderma, 1-3 parts of Bacillus licheniformis, 1-3 parts of Bacillus amyloliquefaciens, 1-3 parts of Vibrio lilacinus, 1-3 parts of Bacillus gelatinus, 1-3 parts of EM bacteria, 1-3 parts of protease, 1-3 parts of saccharifying enzyme, 1-3 parts of amylase, 1-3 parts of phytase, 1-3 parts of pectinase, 1-3 parts of xylanase, 1-3 parts of cellulase, 1-3 parts of fibrinolytic enzyme and 1-3 parts of SOD enzyme;

[0008] The second mixed fertilizer is composed of the following raw materials in parts by weight: 50 to 70 parts of fish protein peptide amino acid water-soluble fertilizer, 20 to 30 parts of trace elements, 5 to 10 parts of rare elements and 1 to 3 parts of brassinolide sterol;

[0009] The third mixed fertilizer is composed of the following raw materials in parts by weight: 30-40 parts of EM flora, 25-35 parts of potassium phosphite, 15-25 parts of ningnanmycin, and 10-20 parts of lentinan oligosaccharide;

[0010] Furthermore, the organic fertilizer is at least one of sheep manure fertilizer, bio-organic fertilizer, or agricultural enzyme fertilizer.

[0011] Furthermore, the trace element is at least one of boron, zinc, manganese, or iron, and the rare element is at least one of titanium or selenium.

[0012] A preparation method of a composition for preventing and treating areca palm yellowing disease includes the following steps:

[0013] Step 1: Preparation of the first mixed fertilizer;

[0014] Step 2: Preparation of the second mixed fertilizer;

[0015] Step 3: Preparation of the third mixed fertilizer.

[0016] Furthermore, the preparation of the first mixed fertilizer includes the following steps:

[0017] S1: Weigh Bacillus subtilis, Trichoderma viride, Bacillus licheniformis, Bacillus amyloliquefaciens, Purpureocillium lilacinum, Bacillus mucilaginosus, and EM flora as required and add them to a horizontal mixer in sequence. Set the rotation speed to 20-40 r / min and the duration to 5-10 min, then take out to obtain a mixed bacteria;

[0018] S2: Weigh protease, glucoamylase, amylase, phytase, pectinase, xylanase, cellulase, plasmin, and SOD enzyme as required and add them to a magnetic stirrer in sequence. Set the rotation speed to 100-300 r / min and the duration to 3-5 min, then take out to obtain a mixed enzyme;

[0019] S3: Weigh the organic fertilizer as required, add it to a horizontal mixer, and sequentially add the mixed bacteria and the mixed enzyme. Set the rotation speed to 40-60 r / min and continue for 15-20 min to complete the preparation of the first mixed fertilizer.

[0020] Furthermore, the preparation of the second mixed fertilizer includes the following steps:

[0021] Step 1: Add brassinolide and ethanol to a stainless steel basin. The mass ratio of brassinolide to ethanol is 1:(10-20), and the concentration of the ethanol is 95%. Stir with a stirrer at a stirring speed of 100-300 r / min for 5-10 min to obtain a brassinolide alcohol solution;

[0022] Step 2: Add rare elements and water into a beaker, with the mass ratio of rare elements to water being (0.01 - 0.03):1. Place the beaker in a water bath and heat it at a temperature of 30 - 40°C for 5 - 10 minutes. During the heating process, use a stirrer to mix to obtain a rare element solution;

[0023] Step 3: Add trace elements and water into a flask, with the mass ratio of rare elements to water being (0.01 - 0.03):1. Place the flask in a water bath and heat it at a temperature of 25 - 30°C for 4 - 6 minutes. During the heating process, use a stirrer to mix to obtain a trace element solution;

[0024] Step 4: Weigh the fish protein peptide amino acid water-soluble fertilizer as needed and add it into a horizontal stirrer. Stir at a speed of 100 - 300 r / min for 50 - 60 minutes. During the stirring process, add the rare element solution, trace element solution, and brassinolide alcohol solution in sequence to complete the preparation of the second mixed fertilizer.

[0025] Further, the preparation of the third mixed fertilizer includes the following steps:

[0026] 1): Weigh potassium phosphite and water as needed and add them into a stainless steel bucket. The mass ratio of potassium phosphite to water is 1:(200 - 300), and use a stirrer to stir at a speed of 100 - 300 r / min for 5 - 8 minutes to obtain a potassium phosphite solution;

[0027] 2): Add ningnanmycin and lentinan oligosaccharide into the stainless steel bucket containing the potassium phosphite solution, and use a stirrer to stir at a speed of 150 - 350 r / min for 8 - 12 minutes to obtain a mixed solution;

[0028] 3): Add EM flora into the stainless steel bucket containing the mixed solution, and use a stirrer to stir at a speed of 80 - 200 r / min for 5 - 10 minutes to complete the preparation of the third mixed fertilizer.

[0029] A control method for a composition for preventing and treating areca palm tree yellowing disease includes the following steps: The first mixed fertilizer is used to activate the soil activity of the roots of areca palm trees. Open trenches in the soil to a depth of 30 cm, apply the first mixed fertilizer, and then backfill the soil. Apply it 2 times a year until the areca palm trees recover green leaves and normal flowering.

[0030] Further, the second mixed fertilizer is used to spray the roots of areca palm trees, spraying once every 15 days until the canopy of the areca palm trees gradually becomes plump.

[0031] Further, the third mixed fertilizer is used to spray the stems and leaves, spraying once every 12 days until the leaves of the areca palm trees are dark green and have a shiny waxy layer.

[0032] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0033] 1. In the present invention, through the combination of organic fertilizer, mixed bacteria and mixed enzymes, the mixed bacteria secrete metabolites to improve the soil aggregate structure, and the mixed enzymes decompose organic matter, release nitrogen, phosphorus, potassium and medium and trace elements, and activate the fixed phosphorus in the soil, alleviating the nutritional imbalance caused by the root system degradation of betel nut. At the same time, Trichoderma in the mixed bacteria directly parasitizes or competitively inhibits soil-borne pathogens, Purpureocillium lilacinum targets and kills root-knot nematodes, and Bacillus subtilis secretes antibacterial peptides to destroy the biofilm of pathogenic bacteria. A probiotic barrier is constructed through the cooperation of multiple strains, reducing the infection risk of the pathogenic source of yellowing disease. And Bacillus licheniformis can produce growth-promoting hormones, stimulate the regeneration of capillary roots, the metabolites of EM flora enhance root activity, SOD enzyme scavenges free radicals, and fibrinolysin improves the permeability of xylem vessels, promoting the transportation of water and nutrients, alleviating leaf yellowing, and achieving the fundamental prevention and control of yellowing disease in betel nut trees.

[0034] 2. In the present invention, the fish protein peptide amino acid water-soluble fertilizer in the second mixed fertilizer is rich in various amino acids, which can provide high-quality nitrogen sources for betel nut trees, promote protein synthesis, and enhance the tree vigor. The medium and trace elements and rare elements participate in many physiological processes of betel nut trees, correcting the yellowing caused by nutrient deficiency. At the same time, brassinolide is a highly efficient plant growth regulator, which can enhance the stress resistance of betel nut trees, improve their resistance to diseases and adverse environments, and promote cell division and elongation, coordinating the distribution of nutrients, making the root system more developed and better absorbing nutrients and water.

[0035] 2. In the present invention, ningnanmycin in the third mixed fertilizer is a broad-spectrum antibiotic, which can inhibit the growth and reproduction of various pathogenic bacteria, and has a preventive and therapeutic effect on diseases caused by bacteria, fungi and viruses. It can directly reduce the damage of pathogenic bacteria to betel nut trees and reduce the incidence of diseases such as yellow leaf disease. And lentinan oligosaccharide can induce plants to produce resistance proteins, activate the self-immune system of betel nut trees, and enhance their ability to resist diseases. Potassium phosphite provides phosphorus and potassium elements, and can also induce plants to produce phytoalexins, enhance the cell wall strength, and inhibit the invasion of pathogenic bacteria. The EM flora can promote the absorption and metabolism of leaf nutrients and improve the leaf health condition. At the same time, under the synergistic action of ningnanmycin and lentinan oligosaccharide, free radicals can be scavenged, reducing the damage of oxidative stress to leaves. By spraying once every 12 days, continuous nutritional support and immune protection are provided, gradually restoring the photosynthetic function and physiological activity of betel nut leaves, fundamentally preventing yellowing disease, and making the leaves return to a healthy state. Detailed implementation manners

[0036] Next, in combination with the embodiments of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0037] Embodiment 1

[0038] A composition for preventing and treating areca palm yellowing disease and a preparation method thereof, including a first mixed fertilizer, a second mixed fertilizer, and a third mixed fertilizer;

[0039] The first mixed fertilizer is composed of the following raw materials in parts by weight: 85 parts of organic fertilizer, 1 part of Bacillus subtilis, 1 part of Trichoderma viride, 1 part of Bacillus licheniformis, 1 part of Bacillus amyloliquefaciens, 1 part of Purpureocillium lilacinum, 1 part of Bacillus mucilaginosus, 1 part of EM flora, 1 part of protease, 1 part of glucoamylase, 1 part of amylase, 1 part of phytase, 1 part of pectinase, 1 part of xylanase, 1 part of cellulase, 1 part of fibrinolysin, and 1 part of SOD enzyme;

[0040] The second mixed fertilizer is composed of the following raw materials in parts by weight: 50 parts of fish protein peptide amino acid water-soluble fertilizer, 20 parts of trace elements, 5 parts of rare elements, and 1 part of brassinolide;

[0041] The third mixed fertilizer is composed of the following raw materials in parts by weight: 30 parts of EM flora, 25 parts of potassium phosphite, 15 parts of ningnanmycin, and 10 parts of lentinan oligosaccharide;

[0042] Furthermore, the organic fertilizer is a biological organic fertilizer.

[0043] Furthermore, the trace element is iron, and the rare element is titanium.

[0044] A preparation method of a composition for preventing and treating areca palm yellowing disease includes the following steps:

[0045] Step 1: Preparation of the first mixed fertilizer;

[0046] Step 2: Preparation of the second mixed fertilizer;

[0047] Step 3: Preparation of the third mixed fertilizer.

[0048] Furthermore, the preparation of the first mixed fertilizer includes the following steps:

[0049] S1: Weigh Bacillus subtilis, Trichoderma viride, Bacillus licheniformis, Bacillus amyloliquefaciens, Purpureocillium lilacinum, Bacillus mucilaginosus, and EM flora as needed and add them to a horizontal mixer in sequence. Set the rotation speed to 20 r / min and the duration to 5 min, and take out to obtain a mixed bacteria;

[0050] S2: Weigh proteases, glucoamylases, amylases, phytases, pectinases, xylanases, cellulases, fibrinolysin and SOD enzymes as needed and add them to a magnetic stirrer in sequence. Set the rotation speed to 100 r / min and the duration to 3 min, then take out to obtain a mixed enzyme solution.

[0051] S3: Weigh organic fertilizers as needed, add them to a horizontal mixer, and then add the mixed bacteria and the mixed enzyme solution in sequence. Set the rotation speed to 40 r / min and the duration to 15 min to complete the preparation of the first mixed fertilizer.

[0052] Further, the preparation of the second mixed fertilizer includes the following steps:

[0053] Step 1: Add brassinolide and ethanol to a stainless-steel basin. The mass ratio of brassinolide to ethanol is 1:10, and the concentration of ethanol is 95%. Stir with a stirrer at a speed of 100 r / min for 5 min to obtain a brassinolide alcohol solution.

[0054] Step 2: Add rare elements and water to a beaker. The mass ratio of rare elements to water is 0.01:1. Place the beaker in a water bath and heat it at 30°C for 5 min. During the heating process, mix with a stirrer to obtain a rare element solution.

[0055] Step 3: Add trace elements and water to a flask. The mass ratio of rare elements to water is 0.01:1. Place the flask in a water bath and heat it at 25°C for 4 min. During the heating process, mix with a stirrer to obtain a trace element solution.

[0056] Step 4: Weigh fish protein peptide amino acid water-soluble fertilizer as needed and add it to a horizontal mixer. Stir at a speed of 100 r / min for 50 min. During the stirring process, add the rare element solution, the trace element solution and the brassinolide alcohol solution in sequence to complete the preparation of the second mixed fertilizer.

[0057] Further, the preparation of the third mixed fertilizer includes the following steps:

[0058] 1): Weigh potassium phosphite and water as needed and add them to a stainless-steel bucket. The mass ratio of potassium phosphite to water is 1:200, and stir with a stirrer at a speed of 100 r / min for 5 min to obtain a potassium phosphite solution.

[0059] 2): Add ningnanmycin and lentinan oligosaccharide to the stainless-steel bucket containing the potassium phosphite solution, and stir with a stirrer at a speed of 150 r / min for 8 min to obtain a mixed solution.

[0060] 3): Add EM flora to the stainless-steel barrel containing the mixed solution, and stir with a stirrer at a stirring speed of 80 r / min for 5 minutes to complete the preparation of the third mixed fertilizer.

[0061] A control method for a composition for preventing and treating areca palm tree yellowing disease, comprising the following steps: The first mixed fertilizer is used to activate the soil activity of the roots of areca palm trees. After opening a trench in the soil to a depth of 30 cm and applying the first mixed fertilizer, then backfill the soil. Apply it 2 times a year until the areca palm trees recover green leaves and normal flowering.

[0062] Furthermore, the second mixed fertilizer is used to spray the roots of areca palm trees, spraying once every 15 days until the canopy of the areca palm trees gradually becomes plump.

[0063] Furthermore, the third mixed fertilizer is used to spray the stems and leaves, spraying once every 12 days until the leaves of the areca palm trees are dark green and have a shiny waxy layer.

[0064] Example 2

[0065] A composition for preventing and treating areca palm tree yellowing disease and its preparation method, comprising a first mixed fertilizer, a second mixed fertilizer and a third mixed fertilizer;

[0066] The first mixed fertilizer is composed of the following raw materials in parts by weight: 87 parts of organic fertilizer, 2 parts of Bacillus subtilis, 2 parts of Trichoderma viride, 2 parts of Bacillus licheniformis, 2 parts of Bacillus amyloliquefaciens, 2 parts of Purpureocillium lilacinum, 2 parts of Bacillus mucilaginosus, 2 parts of EM flora, 2 parts of protease, 2 parts of glucoamylase, 2 parts of amylase, 2 parts of phytase, 2 parts of pectinase, 2 parts of xylanase, 2 parts of cellulase, 2 parts of fibrinolysin and 2 parts of SOD enzyme;

[0067] The second mixed fertilizer is composed of the following raw materials in parts by weight: 60 parts of fish protein peptide amino acid water-soluble fertilizer, 25 parts of trace elements, 7.5 parts of rare elements and 2 parts of brassinolide;

[0068] The third mixed fertilizer is composed of the following raw materials in parts by weight: 35 parts of EM flora, 30 parts of potassium phosphite, 20 parts of ningnanmycin and 15 parts of lentinan oligosaccharide;

[0069] Furthermore, the organic fertilizer is a biological organic fertilizer.

[0070] Furthermore, the trace element is iron and the rare element is titanium.

[0071] A preparation method for a composition for preventing and treating areca palm tree yellowing disease, comprising the following steps:

[0072] Step 1: Preparation of the first mixed fertilizer;

[0073] Step 2: Preparation of the second mixed fertilizer;

[0074] Step 3: Preparation of the third mixed fertilizer.

[0075] Further, the preparation of the first mixed fertilizer includes the following steps:

[0076] S1: Weigh Bacillus subtilis, Trichoderma viride, Bacillus licheniformis, Bacillus amyloliquefaciens, Purpureocillium lilacinum, Bacillus mucilaginosus and EM flora as required and add them to a horizontal mixer in sequence. Set the rotation speed to 30 r / min and the duration to 7.5 min, then take out to obtain a mixed bacteria;

[0077] S2: Weigh protease, glucoamylase, amylase, phytase, pectinase, xylanase, cellulase, plasmin and SOD enzyme as required and add them to a magnetic stirrer in sequence. Set the rotation speed to 200 r / min and the duration to 4 min, then take out to obtain a mixed enzyme;

[0078] S3: Weigh organic fertilizer as required, add it to a horizontal mixer, and sequentially add the mixed bacteria and the mixed enzyme. Set the rotation speed to 50 r / min and the duration to 17.5 min to complete the preparation of the first mixed fertilizer.

[0079] Further, the preparation of the second mixed fertilizer includes the following steps:

[0080] Step 1: Add brassinolide and ethanol to a stainless steel basin. The mass ratio of brassinolide to ethanol is 1:15, and the concentration of ethanol is 95%. Stir with a stirrer at a stirring speed of 200 r / min for 7.5 min to obtain a brassinolide alcohol solution;

[0081] Step 2: Add rare elements and water to a beaker. The mass ratio of rare elements to water is 0.02:1. Place the beaker in a water bath and heat it at 35°C for 7.5 min. During the heating process, mix with a stirrer to obtain a rare element solution;

[0082] Step 3: Add trace elements and water to a flask. The mass ratio of rare elements to water is 0.02:1. Place the flask in a water bath and heat it at 27.5°C for 5 min. During the heating process, mix with a stirrer to obtain a trace element solution;

[0083] Step 4: Weigh fish protein peptide amino acid water-soluble fertilizer as required and add it to a horizontal mixer. The stirring speed is 200 r / min and the stirring time is 55 min. During the stirring process, sequentially add the rare element solution, the trace element solution and the brassinolide alcohol solution to complete the preparation of the second mixed fertilizer.

[0084] Further, the preparation of the third mixed fertilizer includes the following steps:

[0085] 1): Weigh potassium phosphite and water as needed and add them to a stainless-steel barrel. The mass ratio of potassium phosphite to water is 1:250. Stir with a stirrer at a stirring speed of 200 r / min for 6.5 min to obtain a potassium phosphite solution;

[0086] 2): Add ningnanmycin and lentinan oligosaccharide to the stainless-steel barrel containing the potassium phosphite solution, and stir with a stirrer at a stirring speed of 250 r / min for 10 min to obtain a mixed solution;

[0087] 3): Add EM flora to the stainless-steel barrel containing the mixed solution, and stir with a stirrer at a stirring speed of 140 r / min for 7.5 min to complete the preparation of the third mixed fertilizer.

[0088] A control method for a composition for preventing and treating areca palm yellowing disease includes the following steps: The first mixed fertilizer is used to activate the soil activity of the roots of areca palms. After opening a trench in the soil with a depth of 30 cm, apply the first mixed fertilizer and then backfill the soil. Apply it 2 times a year until the areca palms recover green leaves and normal flowering.

[0089] Furthermore, the second mixed fertilizer is used to spray the roots of areca palms, spraying once every 15 days until the canopy of the areca palms gradually becomes plump.

[0090] Furthermore, the third mixed fertilizer is used to spray the stems and leaves, spraying once every 12 days until the leaves of the areca palms are dark green and have a shiny waxy layer.

[0091] Example 3

[0092] A composition for preventing and treating areca palm yellowing disease and its preparation method include a first mixed fertilizer, a second mixed fertilizer, and a third mixed fertilizer;

[0093] The first mixed fertilizer is composed of the following raw materials in parts by weight: 90 parts of organic fertilizer, 3 parts of Bacillus subtilis, 3 parts of Trichoderma, 3 parts of Bacillus licheniformis, 3 parts of Bacillus amyloliquefaciens, 3 parts of Purpureocillium lilacinum, 3 parts of Bacillus mucilaginosus, 3 parts of EM flora, 3 parts of protease, 3 parts of glucoamylase, 3 parts of amylase, 3 parts of phytase, 3 parts of pectinase, 3 parts of xylanase, 3 parts of cellulase, 3 parts of fibrinolysin, and 3 parts of SOD enzyme;

[0094] The second mixed fertilizer is composed of the following raw materials in parts by weight: 70 parts of fish protein peptide amino acid water-soluble fertilizer, 30 parts of trace elements, 10 parts of rare elements, and 3 parts of brassinolide;

[0095] The third mixed fertilizer is composed of the following raw materials in parts by weight: 40 parts of EM flora, 35 parts of potassium phosphite, 25 parts of ningnanmycin, and 20 parts of lentinan oligosaccharide;

[0096] Furthermore, the organic fertilizer is a biological organic fertilizer.

[0097] Further, the trace element is iron and the rare element is titanium.

[0098] A preparation method of a composition for preventing and controlling areca palm yellowing disease includes the following steps:

[0099] Step 1: Preparation of the first mixed fertilizer;

[0100] Step 2: Preparation of the second mixed fertilizer;

[0101] Step 3: Preparation of the third mixed fertilizer.

[0102] Further, the preparation of the first mixed fertilizer includes the following steps:

[0103] S1: Weigh Bacillus subtilis, Trichoderma, Bacillus licheniformis, Bacillus amyloliquefaciens, Purpureocillium lilacinum, Bacillus mucilaginosus and EM flora as required and add them to a horizontal mixer in sequence. Set the rotation speed to 40 r / min and the duration to 10 min, then take out to obtain a mixed bacteria;

[0104] S2: Weigh protease, glucoamylase, amylase, phytase, pectinase, xylanase, cellulase, plasmin and SOD enzyme as required and add them to a magnetic stirrer in sequence. Set the rotation speed to 300 r / min and the duration to 5 min, then take out to obtain a mixed enzyme;

[0105] S3: Weigh organic fertilizer as required, add it to a horizontal mixer, and sequentially add the mixed bacteria and the mixed enzyme. Set the rotation speed to 60 r / min and the duration to 20 min to complete the preparation of the first mixed fertilizer.

[0106] Further, the preparation of the second mixed fertilizer includes the following steps:

[0107] Step 1: Add brassinolide and ethanol to a stainless steel basin. The mass ratio of brassinolide to ethanol is 1:20, and the concentration of ethanol is 95%. Stir with a stirrer at a stirring speed of 300 r / min for 10 min to obtain a brassinolide alcohol solution;

[0108] Step 2: Add the rare element and water to a beaker. The mass ratio of the rare element to water is 0.03:1. Place the beaker in a water bath and heat it at a temperature of 40 °C for 10 min. During the heating process, mix with a stirrer to obtain a rare element solution;

[0109] Step 3: Add the trace element and water to a flask. The mass ratio of the rare element to water is 0.03:1. Place the flask in a water bath and heat it at a temperature of 30 °C for 6 min. During the heating process, mix with a stirrer to obtain a trace element solution;

[0110] Step 4: Weigh the fish protein peptide amino acid water-soluble fertilizer as needed and add it to the horizontal mixer. The stirring speed is 300 r / min, and the stirring time is 60 min. During the stirring process, add the rare element solution, trace element solution, and brassinolide alcohol solution in sequence to complete the preparation of the second mixed fertilizer.

[0111] Further, the preparation of the third mixed fertilizer includes the following steps:

[0112] 1): Weigh potassium phosphite and water as needed and add them to the stainless steel barrel. The mass ratio of potassium phosphite to water is 1:300, and stir with a stirrer at a stirring speed of 300 r / min for 8 min to obtain a potassium phosphite solution;

[0113] 2): Add ningnanmycin and lentinan oligosaccharide to the stainless steel barrel containing the potassium phosphite solution, and stir with a stirrer at a stirring speed of 350 r / min for 12 min to obtain a mixed solution;

[0114] 3): Add EM flora to the stainless steel barrel containing the mixed solution, and stir with a stirrer at a stirring speed of 200 r / min for 10 min to complete the preparation of the third mixed fertilizer.

[0115] A control method for a composition for preventing and treating areca palm tree yellowing disease includes the following steps: The first mixed fertilizer is used to activate the soil activity of the roots of the areca palm tree. After opening a 30-cm deep trench in the soil and applying the first mixed fertilizer, backfill the soil. Apply it 2 times a year until the areca palm tree resumes green leaves and normal flowering.

[0116] Further, the second mixed fertilizer is used to spray the roots of the areca palm tree, spraying once every 15 days until the canopy of the areca palm tree gradually becomes plump.

[0117] Further, the third mixed fertilizer is used to spray the stem and leaves, spraying once every 12 days until the leaves of the areca palm tree are dark green and have a shiny waxy layer.

[0118] Control Group 1. The difference between this control group and Experiment 1 is that: The first mixed fertilizer in this control group uses chemical fertilizers.

[0119] Control Group 2. The difference between this control group and Experiment 1 is that: In the second mixed fertilizer of this control group, brassinolide, trace elements, and rare elements are not pretreated.

[0120] Control Group 3. The difference between this control group and Experiment 1 is that: In the third mixed fertilizer of this control group, the addition is not in sequence during the stirring process.

[0121] Performance test: Fertilize 100 young areca nut seeds with the compositions for preventing and treating areca nut tree chlorosis provided in Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 and Comparative Example 3 respectively, collect the occurrence probability of chlorosis, and record it in the following table:

[0122]

[0123] By comparing and analyzing the relevant data in the table, it can be seen that the composition for preventing and treating areca nut tree chlorosis prepared by the present invention can effectively prevent and treat the chlorosis phenomenon of areca nut trees and promote the normal growth of areca nut trees. Thus, it shows that the preparation method of the composition for improving the prevention and treatment of areca nut tree chlorosis provided by the present invention has a broader market prospect and is more suitable for popularization.

[0124] In the present invention, through the combination of organic fertilizer, mixed bacteria and mixed enzymes, the metabolic products secreted by the mixed bacteria improve the soil aggregate structure, and the mixed enzymes decompose organic matter, release nitrogen, phosphorus, potassium and medium and trace elements and activate the fixed phosphorus in the soil, alleviating the nutritional imbalance caused by the root system degradation of areca nut trees. At the same time, Trichoderma in the mixed bacteria directly parasitizes or competitively inhibits soil-borne pathogens, Purpureocillium lilacinum targets and kills root-knot nematodes, and Bacillus subtilis secretes antibacterial peptides to destroy the biofilm of pathogenic bacteria. A probiotic barrier is constructed through the cooperation of multiple strains, reducing the infection risk of chlorosis pathogenic sources. And Bacillus licheniformis can produce growth-promoting hormones, stimulate the regeneration of capillary roots, the metabolic products of EM flora enhance the root activity, SOD enzymes scavenge free radicals, and fibrinolysin improves the permeability of xylem vessels, promoting the transportation of water and nutrients and alleviating leaf chlorosis, realizing the fundamental prevention and control of areca nut tree chlorosis.

[0125] In the present invention, the fish protein peptide amino acid water-soluble fertilizer in the second mixed fertilizer is rich in various amino acids, which can provide high-quality nitrogen sources for areca nut trees, promote protein synthesis and enhance the tree vigor. The trace elements and rare elements participate in many physiological processes of areca nut trees, correcting the chlorosis caused by nutrient deficiency. At the same time, brassinolide is a highly efficient plant growth regulator, which can enhance the stress resistance of areca nut trees, improve their resistance to diseases and adverse environments, and promote cell division and elongation, coordinate the distribution of nutrients, make the root system more developed, and better absorb nutrients and water.

[0126] In the present invention, ningnanmycin in the third compound fertilizer is a broad-spectrum antibiotic, which can inhibit the growth and reproduction of various pathogenic bacteria and has a preventive and control effect on diseases caused by bacteria, fungi and viruses. It can directly reduce the invasion of pathogenic bacteria on areca trees and reduce the incidence of diseases such as yellow leaf disease. Lentinan oligosaccharide can induce plants to produce resistance proteins, activate the immune system of areca trees themselves, and enhance their ability to resist diseases. Potassium phosphite provides phosphorus and potassium elements, and can also induce plants to produce phytoalexins, enhance the strength of cell walls, and inhibit the invasion of pathogenic bacteria. EM flora can promote the absorption and metabolism of leaf nutrients, improve the health of leaves. At the same time, under the synergistic effect of ningnanmycin and lentinan oligosaccharide, free radicals can be scavenged, and the damage of oxidative stress to leaves can be reduced. By spraying once every 12 days, continuous nutritional support and immune protection are provided, and the photosynthetic function and physiological activity of areca leaves are gradually restored, fundamentally preventing yellowing disease and making the leaves return to a healthy state.

[0127] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. These embodiments are selected and specifically described in this specification to better explain the principle and practical application of the present invention, so that those skilled in the art can understand and utilize the present invention well. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A composition for preventing and treating Areca catechu yellowing disease, characterized in that: It includes a first mixed fertilizer, a second mixed fertilizer and a third mixed fertilizer; The first mixed fertilizer is composed of the following raw materials in parts by weight: 85 to 90 parts of organic fertilizer, 1 to 3 parts of Bacillus subtilis, 1 to 3 parts of Trichoderma, 1 to 3 parts of Bacillus licheniformis, 1 to 3 parts of Bacillus amyloliquefaciens, 1 to 3 parts of Purpurogenum lilacinum, 1 to 3 parts of Bacillus gellingensis, 1 to 3 parts of EM flora, 1 to 3 parts of protease, 1 to 3 parts of saccharifying enzyme, 1 to 3 parts of amylase, 1 to 3 parts of phytase, 1 to 3 parts of pectinase, 1 to 3 parts of xylanase, 1 to 3 parts of cellulase, 1 to 3 parts of fibrinolytic enzyme and 1 to 3 parts of SOD enzyme. The second mixed fertilizer is composed of the following raw materials in parts by weight: 50 to 70 parts of fish protein peptide amino acid water-soluble fertilizer, 20 to 30 parts of trace elements, 5 to 10 parts of rare elements and 1 to 3 parts of brassinolide sterol; The third mixed fertilizer is composed of the following raw materials in parts by weight: 30 to 40 parts of EM bacteria, 25 to 35 parts of potassium phosphite, 15 to 25 parts of Ningnanmycin and 10 to 20 parts of Lentinan oligosaccharide; 2. The composition for preventing and treating Areca catechu yellowing disease according to claim 1, characterized in that: The organic fertilizer is at least one of sheep manure fertilizer, biological organic fertilizer or agricultural enzyme fertilizer.

3. The composition for preventing and treating Areca catechu yellowing disease according to claim 1, characterized in that: The trace element is at least one of boron, zinc, manganese or iron, and the rare element is at least one of titanium or selenium.

4. A method for preparing a composition for preventing and treating Areca catechu yellowing disease, characterized in that: The composition for preventing and treating betel nut yellowing disease according to any one of claims 1 to 3 is used, comprising the following steps: Step 1: preparing the first mixed fertilizer; Step 2: preparing the second mixed fertilizer; Step 3: Preparation of the third mixed fertilizer.

5. The method for preparing the composition for preventing and treating Areca catechu yellowing disease according to claim 4, characterized in that: The first mixed fertilizer preparation comprises the following steps: S1: Weigh Bacillus subtilis, Trichoderma, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus lilacinus, Bacillus mucilaginosus and EM bacteria as needed and add them into a horizontal mixer in sequence, set the speed to 20-40 r / min, last for 5-10 min, and take out to obtain mixed bacteria; S2: Weigh protease, saccharifying enzyme, amylase, phytase, pectinase, xylanase, cellulase, fibrinolytic enzyme and SOD enzyme as needed and add them into the magnetic stirrer in sequence, set the speed to 100-300 r / min, last for 3-5 min, and take out to obtain the mixed enzyme; S3: Weigh the organic fertilizer as needed, add it into the horizontal mixer, and add the mixed bacteria and mixed enzymes in sequence, set the speed to 40-60r / min, and continue for 15-20min to complete the preparation of the first mixed fertilizer.

6. The method for preparing the composition for preventing and treating Areca catechu yellowing disease according to claim 4, characterized in that: The second mixed fertilizer preparation comprises the following steps: Step 1: adding brassinosteroid and ethanol into a stainless steel basin, wherein the mass ratio of brassinosteroid to ethanol is 1:(10-20), the concentration of ethanol is 95%, and stirring is performed with a stirrer at a speed of 100-300 r / min for 5-10 min to obtain a brassinosteroid alcohol solution; Step 2: Add rare elements and water into a beaker, the mass ratio of rare elements to water is (0.01-0.03):1, put the beaker into a water bath, heat at 30-40°C for 5-10 minutes, and use a stirrer to mix during the heating process to obtain a rare element solution; Step 3: Add trace elements and water into the flask, the mass ratio of rare elements to water is (0.01-0.03):1, put the flask into a water bath, heat at 25-30°C for 4-6 minutes, and use a stirrer to mix during the heating process to obtain a trace element solution; Step 4: Weigh fish protein peptide amino acid water-soluble fertilizer as needed and add it into a horizontal mixer at a stirring speed of 100-300 r / min for 50-60 min. During the stirring process, add rare element solution, trace element solution and brassinosteroid alcohol solution in sequence to complete the preparation of the second mixed fertilizer.

7. The method for preparing the composition for preventing and treating Areca catechu yellowing disease according to claim 4, characterized in that: The preparation of the third mixed fertilizer comprises the following steps: 1): Weigh potassium phosphite and water as needed and add them into a stainless steel barrel, the mass ratio of potassium phosphite to water is 1: (200-300), and stir with a stirrer at a stirring speed of 100-300 r / min and a stirring time of 5-8 min to obtain a potassium phosphite solution; 2) Add Ningnanmycin and Lentinan oligosaccharide into a stainless steel barrel filled with potassium phosphite solution, and stir with a stirrer at a speed of 150 to 350 r / min for 8 to 12 min to obtain a mixed solution; 3): Add EM bacteria into the stainless steel barrel containing the mixed solution, and stir with a stirrer at a speed of 80 to 200 r / min and a stirring time of 5 to 10 min to complete the preparation of the third mixed fertilizer.

8. A method for controlling a composition for controlling Areca catechu yellowing disease, characterized in that: The composition for preventing and controlling the chlorosis of areca palm trees according to any one of claims 1 to 7 is used, comprising the following steps: the first mixed fertilizer is used to activate the soil activity at the roots of the areca palm trees, a 30 cm deep trench is dug in the soil, the first mixed fertilizer is spread, and then the soil is returned to the original state, and the fertilizer is applied twice a year until the areca palm trees regain their green leaves and bloom normally.

9. The control method of the composition for controlling Areca catechu yellowing disease according to claim 8, characterized in that: The second mixed fertilizer is used for spraying the roots of the betel nut tree, once every 15 days, until the crown of the betel nut tree gradually recovers its fullness.

10. The control method of the composition for controlling Areca catechu yellowing disease according to claim 8, characterized in that: The third mixed fertilizer is used for spraying stems and leaves, once every 12 days, until the leaves of the betel nut tree are dark green and have a shiny wax layer.

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