Culture experiment table for myocardial cell hypoxia reoxygenation experiment

Abstract

The invention discloses a culture experiment table for a myocardial cell hypoxia reoxygenation experiment, and relates to the field of cell culture experiment tables. The device comprises an experiment box set and a sealing shell fixedly installed on the rear end face of the experiment box set, the experiment box set comprises a bottom box part and a top box part arranged above the bottom box part, a partition inclined plate is fixedly installed in the middle of the bottom box part, and the space in the bottom box part is divided into a first equipment area and a storage area by the partition inclined plate; a vertical partition plate is fixedly installed in the middle of the top box part and divides the space in the top box part into a refrigeration area and a second equipment area, and a moving assembly is installed in the refrigeration area. The experiment box group is designed into a structure in which the bottom box part and the top box part are matched, the experiment box group is divided into four spaces with different functions through the arrangement of the separation inclined plate and the vertical separation plate, and the motor group is installed through the first equipment area, so that the motor group can stably drive the moving assembly to rotate and work.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture experimental platforms, and specifically relates to a culture experimental platform for myocardial cell anoxia / reoxygenation experiments. Background Art

[0002] Cardiovascular diseases are common diseases that affect the health of the elderly, so people are paying more and more attention to cardiovascular diseases. Ischemic heart disease remains a major threat to life, and myocardial ischemia / reperfusion injury is the main cause of high morbidity and mortality. Anoxia / reoxygenation (A / R) injury is an inflammatory process that leads to an overload of calcium ion concentration, damage to mitochondrial membrane potential, and energy metabolism disorders, thereby changing the cell structure and function and reducing cell activity. Therefore, the development of new strategies to prevent myocardial cell death after ischemia and reperfusion is necessary to improve the clinical efficacy of patients with ischemic heart disease, and the myocardial cell anoxia / reoxygenation experiment is a common means to study cardiovascular diseases.

[0003] In view of the above related technologies, the inventor found that the existing culture experimental platforms for cell experiments can only be used as experimental platforms during use. During the experiment, the experimenters need to constantly move back and forth between the refrigerator and the instrument storage cabinet to pick up and use experimental instruments and cell samples. Such operation is relatively cumbersome, with low efficiency, and there is also a risk of dropping during the picking and placing process. At the same time, the height of the operating table is determined and cannot meet the comfortable use of different experimenters. Summary of the Invention

[0004] In order to achieve easy picking and placing of experimental instruments and cell samples, and to facilitate different experimenters to adjust the height for use, the present application provides a culture experimental platform for myocardial cell anoxia / reoxygenation experiments.

[0005] The present invention is implemented as follows: A culture experimental platform for myocardial cell anoxia / reoxygenation experiments, including a group of experimental boxes and a sealing shell fixedly installed on the rear end face of the experimental box group. The experimental box group includes a bottom box part and a top box part arranged above the bottom box part. A partition inclined plate is fixedly installed in the middle of the bottom box part, and the partition inclined plate divides the space in the bottom box part into an equipment area one and a storage area. A vertical partition plate is fixedly installed in the middle of the top box part, and the vertical partition plate divides the space in the top box part into a refrigeration area and an equipment area two. A moving component is installed in the refrigeration area, and a plurality of cell storage mechanisms are evenly installed on the upper end face of the moving component. A picking and placing groove corresponding to the cell storage mechanism is opened on the upper end face of the experimental box group, and a corresponding sterilization shell is arranged above the picking and placing groove. A plurality of experimental tables are also slidably installed on the upper end face of the experimental box group, and a driving member for driving the experimental table to lift is fixedly installed in the equipment area two. A cooling component for cooling the refrigeration area is fixedly installed on the sealing shell.

[0006] By adopting the above technical solution, the culture experimental bench is designed as a structure in which an experimental box group and a sealing shell are matched. When it is easy to use, it can be installed on the rear end face of the experimental box group through the sealing shell for sealing and shielding use, ensuring that equipment can be installed inside the experimental box, sealed storage, and refrigerated cell samples can be achieved. And by designing the experimental box group into two parts: a bottom box part and a top box part, it is ensured that different usage requirements can be met through the bottom box part and the top box part during use. By fixedly installing a partition inclined plate in the middle of the bottom box part, the bottom box part is divided into an equipment area one and a storage area by the partition inclined plate. During use, corresponding equipment structures can be installed in the equipment area one, and the storage area is provided to ensure that items can be stored at the lower end of the experimental bench during use. Moreover, the inclined installation of the partition inclined plate can ensure that the storage space of the storage area is maximally increased. By installing a vertical partition in the top box part, the space in the top box part is divided into a refrigeration area and an equipment area two by the vertical partition. In this way, cell samples and experimental equipment to be refrigerated can be stored in the refrigeration area during use, and the equipment area two is provided to install a driving member, facilitating the stable driving of the experimental bench to lift and adjust through the driving member during use, ensuring that different height usage requirements can be met during the experiment. By installing a moving component in the refrigeration area, it is ensured that the cell storage mechanism is driven to move in a circular motion in the refrigeration area through the moving component, realizing the correspondence between different cell storage mechanisms and the pick-and-place slots on the upper end face of the experimental box group, facilitating the experimenters operating on the experimental bench to easily pick and place different cell samples and experimental equipment. By arranging a corresponding sterilization shell above the pick-and-place slots, it is ensured that cell samples or experimental equipment can be directly taken out from the sterilization shell when picking and placing, and the sterilization shell is provided to ensure the sterilization of cell samples or experimental equipment to ensure the safety of the experiment. By slidably installing a number of experimental benches on the upper end face of the experimental box group, it is ensured that a number of experimental benches can be spliced with each other for experimental operations. At the same time, the experimental bench can be controlled to use at a height through the driving member during use, facilitating different experimenters to easily conduct experimental operations. By fixedly installing a temperature reduction component on the sealing shell, it is ensured that the temperature of the refrigeration area is controlled through the temperature reduction component, ensuring better storage of cell samples and experimental equipment.

[0007] Furthermore, a horizontal support is installed in the equipment area one, the horizontal support is fixedly connected to the equipment area one, a number of partition plates are installed in the storage area, the partition plates divide the storage area into a number of different storage spaces, and an experimental box door corresponding to each storage space is installed on the front end face of the storage area. A number of equipment feet are also evenly installed on the lower end face of the storage area.

[0008] By adopting the above technical solution, a horizontal support is fixedly installed in the first equipment area to ensure that the mechanism for driving and moving the component can be supported and installed by the horizontal support during use. And by installing a number of partition plates in the storage area, it is ensured that the storage area is divided into several different storage spaces by the partition plates, which is convenient for storing different items for use. And through the experimental box door at the front end of the storage area, it is ensured that different storage spaces can be used through different experimental box doors during use. And by installing a number of equipment feet on the lower end face of the storage area, it is ensured that the equipment can be stably supported on the ground by the equipment feet during use.

[0009] Furthermore, the sealing shell includes a back cover, sealing side plates and a buckling cover for sealing the first equipment area. The sealing side plates are installed on both sides of the back cover, and the sealing side plates and the back cover are integrally formed. The buckling cover includes a main cover plate, triangular cover caps and a positioning plate for installing the cooling component. The main cover plate is fixedly installed on the front end face of the back cover. The triangular cover caps are arranged on both sides of the main cover plate. The positioning plate is installed on the front end face of the main cover plate, and the main cover plate, the triangular cover caps and the positioning plate are integrally formed. The cooling component includes a liquid nitrogen bottle and a shunt nozzle. The liquid nitrogen bottle is fixedly installed on the positioning plate. The shunt nozzle is fixedly installed on the sealing side plate, and the shunt nozzle is connected to the air outlet of the liquid nitrogen bottle.

[0010] By adopting the above technical solution, the sealing shell is designed into a structure in which the back cover, the sealing side plates and the buckling cover cooperate with each other. Thus, the sealing side plates and the buckling cover can be fixedly installed by the back cover. In this way, during installation, the back cover and the two groups of sealing side plates can be used to cooperate to block and seal the rear end and the side surface of the top box part. And the buckling cover is used to seal the bottom box part. And by designing the buckling cover into a structure in which the main cover plate, the triangular cover caps and the positioning plate cooperate with each other, it is easy to seal the rear end face and the side surface of the bottom box part by the main cover plate and the triangular cover caps during use. And the setting of the positioning plate ensures the stable installation and use of the liquid nitrogen bottle.

[0011] Furthermore, a guiding slide rail is fixedly installed on the inner side surface of the sealing side plate. A horizontal support is installed on the guiding slide rail. One end of the horizontal support is slidably connected to the guiding slide rail, and the other end of the horizontal support is fixedly installed with a baffle cover that cooperates with the picking and placing groove. An electric cylinder for driving the front end of the horizontal support to slide is also fixedly installed on the inner side surface of the sealing side plate. A display screen is embedded in the upper end face of the baffle cover.

[0012] By adopting the above technical solution, a guiding slide rail is fixedly installed on the inner side surface of the sealed side plate, so that when in use, the horizontal bracket can be installed by driving the slide rail, and the baffle is fixedly installed through the arrangement of the horizontal bracket. In this way, when the electric cylinder drives the horizontal bracket to move back and forth, the baffle can be driven to cooperate with the pick-and-place slot, realizing the shielding and opening of the pick-and-place slot. At the same time, by embedding a display screen on the baffle, it is ensured that the display screen can be used to display in real time which cell storage mechanism is directly below the pick-and-place slot at this time. By numbering different cell storage mechanisms, it is convenient to determine the corresponding equipment before opening the pick-and-place slot, which is conducive to better precise picking and placing operations.

[0013] Further, the moving component includes a driving pulley, a driven pulley, a transmission wide belt, and a positioning disk for installing the cell storage mechanism. The driving pulley and the driven pulley are both rotatably installed in the refrigerated area, and a motor group for driving the driving pulley to rotate is fixedly installed on the horizontal support base. The two ends of the transmission wide belt are respectively installed on the driving pulley and the driven pulley, and the positioning disks are evenly fixed on the transmission wide belt.

[0014] By adopting the above technical solution, by designing the moving component into a structure in which the driving pulley, the driven pulley, the transmission wide belt, and the positioning disk cooperate with each other, it is convenient to support and install the transmission wide belt through the driving pulley and the driven pulley when in use. When in use, the motor group fixedly installed on the horizontal support base drives the driving pulley to rotate, thereby driving the transmission wide belt to rotate stably, realizing the synchronous movement of the positioning disk. During the rotation process, different positioning disks can be moved to directly below the pick-and-place slot, thereby realizing the movement of different cell storage mechanisms into the pick-and-place slot.

[0015] Further, the cell storage mechanism includes a positioning ring seat, a placement shell, and a lifting component. The positioning ring seat is fixedly installed on the upper end surface of the positioning disk, the placement shell is slidably installed in the positioning ring seat, the lifting component is vertically installed at the center of the positioning ring seat, and the lifting component is used to adjust the height of the placement shell in the positioning ring seat. A test tube ring seat is installed in the placement shell, and the test tube ring seat is rotatably connected to the placement shell.

[0016] By adopting the above technical solution, by designing the cell storage mechanism into a structure in which the positioning ring seat, the placement shell, and the lifting component cooperate with each other, it is easy to fixedly install it on the positioning disk through the positioning ring seat when in use, and then install the placement shell through the positioning ring seat, ensuring that the test tube ring seat is rotatably installed through the placement shell. During use, test tubes can be stably placed through the test tube ring seat, and different use positions can also be adjusted by rotation during picking and placing. In actual use, the height of the placement shell can also be controlled by the lifting component. In this way, after the cell storage mechanism rotates to below the pick-and-place slot, the placement shell can be lifted from the pick-and-place slot through the lifting component, facilitating direct picking and placing by the experimenter.

[0017] Furthermore, the positioning ring seat includes a ring frame plate, a back arc plate, a front arc plate, and an inner frame for installing the lifting assembly. A set of back arc plates and two sets of front arc plates are evenly installed on the upper end surface of the ring frame plate along the circumferential direction, and both the back arc plate and the front arc plate are fixedly connected to the ring frame plate. A limiting plate is integrally formed on the upper end surface of the front arc plate. The inner frame is installed in the middle of the ring frame plate and is integrally formed with the ring frame plate. The placement shell includes a disc plate and an arc-shaped baffle. The arc-shaped baffle is arranged on the upper end surface of the disc plate and is integrally formed with the disc plate. A threaded sleeve is fixedly installed at the center of the disc plate. The lifting assembly includes a driving motor 1 and a threaded rod that cooperates with the threaded sleeve. The lower end of the threaded rod is fixedly intercepted at the output end of the driving motor 1.

[0018] By adopting the above technical solution, by designing the positioning ring seat into a structure that combines a ring frame plate, a back arc plate, a front arc plate, and an inner frame, it is ensured that the ring frame plate can be fixed to the positioning disc during use. By arranging a set of back arc plates and two sets of front arc plates on the ring frame plate, the placement shell can be limited by the back arc plate and the front arc plate, ensuring that the placement shell can only slide up and down in the positioning ring seat. By fixedly installing the inner frame in the middle of the ring frame plate, it is ensured that the lifting assembly can be fixedly installed through the inner frame during use, facilitating the stable driving of the placement shell by the lifting assembly. By arranging a limiting plate on the upper end surface of the front arc plate, it is ensured that the lifting assembly is limited by the limiting plate to prevent the lifting assembly from rotating during the lifting process. By designing the placement shell into a structure that combines a disc plate and an arc-shaped baffle, it is easy to install the test tube ring seat through the cooperation of the disc plate and the arc-shaped baffle during use, facilitating the stable rotational installation of the test tube ring seat in the placement shell. By designing the lifting assembly into a structure that combines a driving motor 1 and a threaded rod, it is ensured that the driving motor 1 can drive the threaded rod to rotate during use, and then drive the placement shell to lift through the cooperation of the threaded rod and the threaded sleeve.

[0019] Furthermore, the test tube ring seat includes an annular seat, a placement arc plate 1, a vertical plate, a threaded push rod, and a placement arc plate 2. The annular seat is installed on the upper end surface of the disc plate and is rotatably connected to the arc-shaped baffle. The placement arc plate 1 is fixedly installed on the upper end surface of the annular seat. The vertical plate is fixedly installed on the upper end surface of the annular seat. The threaded push rod is threadedly connected to the vertical plate. The placement arc plate 2 is arranged opposite to the placement arc plate 1, and the placement arc plate 2 is rotatably installed at one end of the threaded push rod.

[0020] By adopting the above technical solution, by designing the test tube ring seat into a structure that combines an annular seat, a placing arc plate I, a vertical plate, a threaded push rod and a placing arc plate II, it can be easily rotated and installed in the arc-shaped baffle through the annular seat during use, and the threaded push rod can be installed through the vertical plate on the annular seat. After the placing arc plate II is rotatably installed at the head of the threaded push rod, it can cooperate with the placing arc plate I. During use, the distance between the placing arc plate I and the placing arc plate II can be changed by rotating the threaded push rod, thereby facilitating the insertion and placement of test tubes of different sizes.

[0021] Furthermore, the sterilization shell includes a main shell and a transparent arc cover that can be slid open. The main shell is fixedly installed on the upper end surface of the experimental box group, the transparent arc cover is slidably installed in the main shell, and a sterilization lamp is fixedly installed on the inner side surface of the main shell.

[0022] By adopting the above technical solution, by designing the sterilization shell into a structure that combines the main shell and the transparent arc cover, the transparent arc cover can be slid open during easy access and operation, and can be slid closed when not in use. Moreover, before access, the main shell can be sterilized by the sterilization lamp on the inner side surface of the main shell, which can effectively ensure and increase the safety during the access process.

[0023] Furthermore, the experimental table includes a clean table board, a concave bracket and a vertical rod frame. The concave bracket is fixedly installed on the lower end surface of the clean table board, the vertical rod frame is fixedly installed at both ends of the concave bracket, and the lower end of the vertical rod frame is slidably installed in the equipment area II. A mating rack is also fixedly installed on one side of the vertical rod frame. A storage groove for receiving the concave bracket is provided on the upper end surface of the top box part. The driving member includes an inner seat, a mounting seat and a transmission shaft. The inner seat is fixedly installed in the equipment area II, and both ends of the inner seat clamp the vertical rod frame. The mounting seat is fixed in the middle of the inner seat. The transmission shaft is rotatably installed on the mounting seat, and driving gears meshing with the mating rack are fixedly installed at both ends of the transmission shaft. A driving motor II for driving the transmission shaft to rotate is also fixedly installed on the lower end surface of the mounting seat.

[0024] By adopting the above technical solution, the experimental bench is designed into a structure in which a clean tabletop, a concave bracket, and a vertical rod bracket are matched. When in use, the clean tabletop can be supported by the concave bracket at the lower end, and by installing a vertical rod bracket on the concave bracket, it is ensured that when in use, it can be inserted into the second area of the equipment from the upper end surface of the top box part. Without adjusting the clean tabletop, the vertical rod bracket can be completely inserted into the top box part during the experiment. When the height needs to be adjusted, the driving motor two can be started to drive the transmission shaft to rotate, and then the driving gears at both ends of the transmission shaft are used to drive the mating rack to move upward, so as to drive the vertical rod bracket and the clean tabletop to move upward synchronously, ensuring that the clean tabletop can be stably raised for use. By providing a storage groove on the upper end surface of the top box part for receiving the concave bracket, the concave bracket can be received into the storage groove when it is not necessary to raise the use, which is convenient for the clean tabletop to be stably used by fitting on the upper end surface of the top box part.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) In this application, the experimental box group is designed into a structure in which the bottom box part and the top box part are matched, and the experimental box group is divided into four spaces with different functions by the partition inclined plate and the vertical partition plate. The motor group is installed in the first area of the equipment, which is convenient for the motor group to stably drive the moving component to rotate. The storage area is provided to store different items respectively. At the same time, the refrigeration area is provided to store the cells and equipment that need to be refrigerated during the experiment. Integrating the refrigeration area into the experimental box group can effectively improve the convenience of use for the experimental personnel. The second area of the equipment is provided to install the driving member, and it is convenient for the vertical rod bracket at the lower end of the experimental bench to be stably collected, so that the use height of the experimental bench can be controlled by the driving member during use, which is easy to meet the use needs of different experimental personnel, so as to realize the flexible storage of experimental equipment and cells and the comfortable use of different experimental personnel.

[0026] (2) In this application, a sealing shell is installed on the experimental box group, which is convenient to ensure that each space in the experimental box group can be sealed for use through the sealing shell. By installing a horizontal bracket and a baffle on the seal to cooperate with the access slot on the experimental box group, it is ensured that the access slot can be automatically opened by the electric cylinder during access and automatically closed when not in use. By fixedly installing a sterilization shell on the experimental box group, it is ensured that the position of the access slot can be better sterilized, which can effectively improve the safety of accessing cells or equipment, so as to achieve the purpose of safe access and use.

[0027] (3) In this application, by installing a moving component in the refrigerated area, when in use, the positioning disk on the moving component can be conveniently used to support and install the cell storage mechanism. During the use process, different cell storage mechanisms can be driven by the moving component to correspond to the picking and placing slot. In this way, different items can be stored using different cell storage mechanisms. And by designing the cell storage mechanism into a structure in which a positioning ring base, a placing shell, and a lifting component cooperate, after the cell storage mechanism is aligned with the picking and placing slot, the placing shell can be lifted from the picking and placing slot through the lifting component, facilitating the experimenter to perform the picking and placing operations more easily, thereby achieving the purpose of easily using different cell storage mechanisms for storage. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the embodiments. It should be understood that the following drawings only show some embodiments of the present invention, and thus should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings.

[0029] Figure 1 is the overall structural schematic diagram in the embodiment of the present invention; Figure 2 is Figure 1 the exploded structural schematic diagram of the device shown when the sterilization shell and the experimental table are not installed; Figure 3 is Figure 2 the side view of the device shown; Figure 4 is the rear three-dimensional view of the experimental box group in the embodiment of the present invention; Figure 5 is Figure 4 the front view of the device shown; Figure 6 is Figure 5 the sectional view of the device shown along the A-A direction; Figure 7 is Figure 4 the side view of the device shown; Figure 8 is the front three-dimensional view of the sealing shell and the cooling component cooperating in the embodiment of the present invention; Figure 9 is the rear three-dimensional view of the sealing shell and the cooling component cooperating in the embodiment of the present invention; Figure 10 is Figure 8 the front view of the device shown; Figure 11 is Figure 8 the top view of the device shown; Figure 12It is a perspective view of the sterilization shell in the embodiment of the present invention; Figure 13 It is a perspective view of the cell storage mechanism in the embodiment of the present invention; Figure 14 It is Figure 13 A perspective view of the cooperation between the positioning ring seat and the lifting component in the device shown; Figure 15 It is Figure 13 A perspective view of the placement shell in the device shown; Figure 16 It is Figure 15 A top view of the device shown; Figure 17 It is a perspective view of the cooperation between the experimental table and the driving part in the embodiment of the present invention; Figure 18 It is Figure 17 A front view of the device shown; Figure 19 It is a perspective view of the driving part in the embodiment of the present invention; Figure 20 It is Figure 19 A front view of the device shown.

[0030] In the figure: 1. Experimental box group; 10. Pick-and-place groove; 11. Bottom box part; 111. Equipment area 1; 112. Storage area; 113. Horizontal support; 114. Partition board; 115. Experimental box door; 116. Equipment support feet; 117. Motor group; 12. Top box part; 121. Refrigeration area; 122. Equipment area 2; 123. Storage groove; 13. Partition inclined plate; 14. Vertical partition board; 2. Sealing shell; 21. Back cover; 22. Sealing side plate; 221. Guide slide rail; 222. Horizontal bracket; 223. Cover; 224. Electric cylinder; 225. Display screen; 23. Buckle cover; 231. Main cover plate; 232. Triangular cover; 233. Positioning plate; 3. Moving component; 31. Driving pulley; 32. Driven pulley; 33. Transmission wide belt; 34. Positioning disk; 4. Cell storage mechanism; 41. Positioning ring seat; 411. Ring frame plate; 412. Back arc plate; 413. Front arc plate; 414. Inner frame; 415. Limiting plate; 42. Placement shell; 421. Disk plate; 422. Arc-shaped baffle; 423. Threaded sleeve; 43. Lifting component; 431. Driving motor 1; 432. Threaded rod; 44. Test tube ring seat; 441. Ring seat; 442. Placement arc plate 1; 443. Vertical plate; 444. Threaded push rod; 445. Placement arc plate 2; 5. Sterilization shell; 51. Main shell; 52. Transparent arc cover; 6. Experimental table; 61. Clean table board; 62. Concave bracket; 63. Vertical rod frame; 631. Matching rack; 7. Driving part; 71. Inner seat; 72. Mounting seat; 73. Transmission shaft; 731. Driving gear; 74. Driving motor 2; 8. Cooling component; 81. Liquid nitrogen bottle; 82. Shunt nozzle. Detailed implementation manners

[0031] To make the objectives, technical solutions and advantages of the implementation manners of the present invention clearer, the technical solutions in the implementation manners of the present invention will be clearly and completely described below with reference to the accompanying drawings in the implementation manners of the present invention. Obviously, the described implementation manners are some but not all of the implementation manners of the present invention. All other implementation manners obtained by those of ordinary skill in the art based on the implementation manners in the present invention without making creative efforts belong to the scope of protection of the present invention. Therefore, the following detailed description of the implementation manners of the present invention provided in the accompanying drawings is not intended to limit the scope of the claimed present invention, but merely represents selected implementation manners of the present invention.

[0032] Example 1 Refer to Figure 1 、 Figure 2 、 Figure 3 and Figure 7As shown in the figure, a culture test bench for cardiomyocyte hypoxia-reoxygenation experiments includes an experimental box group 1 and a sealing shell 2 fixedly installed on the rear end face of the experimental box group 1. The experimental box group 1 includes a bottom box part 11 and a top box part 12 arranged above the bottom box part 11. A partition inclined plate 13 is fixedly installed in the middle of the bottom box part 11. The partition inclined plate 13 divides the space in the bottom box part 11 into an equipment area 111 and a storage area 112. A vertical partition plate 14 is fixedly installed in the middle of the top box part 12. The vertical partition plate 14 divides the space in the top box part 12 into a refrigeration area 121 and an equipment area 122. A moving component 3 is installed in the refrigeration area 121. A plurality of cell storage mechanisms 4 are evenly installed on the upper end face of the moving component 3. A placement and extraction groove 10 corresponding to the cell storage mechanism 4 is opened on the upper end face of the experimental box group 1, and a corresponding sterilization shell 5 is arranged above the placement and extraction groove 10. A plurality of test benches 6 are also slidably installed on the upper end face of the experimental box group 1, and a driving component 7 for driving the test bench 6 to lift is fixedly installed in the equipment area 122. A cooling component 8 for cooling the refrigeration area 121 is fixedly installed on the sealing shell 2.By designing the culture test bench 6 into a structure where the experimental box group 1 and the sealing shell 2 cooperate, when it is easy to use, it can be installed on the rear end face of the experimental box group 1 through the sealing shell 2 for sealing and shielding use, ensuring that equipment can be installed inside the experimental box, sealed storage, and refrigerated cell samples can be achieved. And by designing the experimental box group 1 into a two-part structure of the bottom box part 11 and the top box part 12, it is ensured that different usage requirements can be met through the bottom box part 11 and the top box part 12 during use. By fixedly installing a partition inclined plate 13 in the middle of the bottom box part 11, the bottom box part 11 is divided into an equipment area 111 and a storage area 112 by the partition inclined plate 13. During use, corresponding equipment structures can be installed through the equipment area 111, and by setting the storage area 112, it is ensured that items can be stored at the lower end of the test bench 6 during use. Moreover, by the inclined installation of the partition inclined plate 13, the storage space of the storage area 112 can be maximally increased. By installing a vertical partition 14 in the top box part 12, the space in the top box part 12 is divided into a refrigeration area 121 and an equipment area 122 by the vertical partition 14. In this way, cell samples and experimental equipment to be refrigerated can be stored through the refrigeration area 121 during use, and the driving part 7 is installed through the setting of the equipment area 122, facilitating the stable driving of the test bench 6 to lift and adjust through the driving part 7 during use, ensuring that different height usage requirements can be met during the experiment. By installing a moving component 3 in the refrigeration area 121, it is ensured that the cell storage mechanism 4 is driven to move in a circular motion in the refrigeration area 121 through the moving component 3, realizing the correspondence of different cell storage mechanisms 4 with the pick-and-place slot 10 on the upper end face of the experimental box group 1, facilitating the experimenters operating on the test bench 6 to easily pick and place different cell samples and experimental equipment. By setting a corresponding sterilization shell 5 above the pick-and-place slot 10, it is ensured that cell samples or experimental equipment can be directly taken out from the sterilization shell 5 when picking and placing, and the setting of the sterilization shell 5 is used to ensure the sterilization of cell samples or experimental equipment to ensure the safety of the experiment. By slidably installing several test benches 6 on the upper end face of the experimental box group 1, it is ensured that several test benches 6 can be spliced with each other for experimental operations. At the same time, during use, the test bench 6 can control the usage height through the driving part 7, facilitating different experimenters to easily conduct experimental operations. By fixedly installing a temperature reduction component 8 on the sealing shell 2, it is ensured that the temperature of the refrigeration area 121 is controlled through the temperature reduction component 8, ensuring better storage of cell samples and experimental equipment.

[0033] Refer to Figure 2 、 Figure 3 、 Figure 4 and Figure 5As shown in the figure, a horizontal support 113 is installed in equipment area 111. The horizontal support 113 is fixedly connected to equipment area 111. A number of partition plates 114 are installed in storage area 112. The partition plates 114 divide storage area 112 into several different storage spaces. Moreover, experimental chamber doors 115 corresponding to each storage space are installed on the front end face of storage area 112. A number of equipment feet 116 are evenly installed on the lower end face of storage area 112. By fixedly installing the horizontal support 113 in equipment area 111, it is ensured that the mechanism for supporting and installing the driving and moving component 3 can be supported by the horizontal support 113 during use. And by installing a number of partition plates 114 in storage area 112, it is ensured that storage area 112 is divided into several different storage spaces by the partition plates 114, which is convenient for storing different items for use. And through the experimental chamber doors 115 on the front end face of the storage area, it is ensured that different storage spaces can be used through different experimental chamber doors 115 during use. While by installing a number of equipment feet 116 on the lower end face of storage area 112, it is ensured that the equipment can be stably supported on the ground through the equipment feet 116 during use.

[0034] Refer to Figure 8 、 Figure 9 and Figure 10As shown, the sealing shell 2 includes a back cover 21, sealing side plates 22, and a fastening cover 23 for sealing the equipment area 111. The sealing side plates 22 are installed on both sides of the back cover 21, and the sealing side plates 22 and the back cover 21 are integrally formed. The fastening cover 23 includes a main cover plate 231, triangular sealing covers 232, and a positioning plate 233 for installing the cooling component 8. The main cover plate 231 is fixedly installed on the front end face of the back cover 21. The triangular sealing covers 232 are arranged on both sides of the main cover plate 231. The positioning plate 233 is installed on the front end face of the main cover plate 231, and the main cover plate 231, triangular sealing covers 232, and positioning plate 233 are integrally formed. The cooling component 8 includes a liquid nitrogen bottle 81 and a flow splitting nozzle 82. The liquid nitrogen bottle 81 is fixedly installed on the positioning plate 233. The flow splitting nozzle 82 is fixedly installed on the sealing side plate 22, and the flow splitting nozzle 82 is connected to the gas outlet of the liquid nitrogen bottle 81. By designing the sealing shell 2 into a structure in which the back cover 21, sealing side plates 22, and fastening cover 23 cooperate, the sealing side plates 22 and the fastening cover 23 can be fixedly installed through the back cover 21. In this way, during installation, the back cover 21 and the two groups of sealing side plates 22 can cooperate to shield and seal the rear end and side of the top box part 12, and the fastening cover 23 is provided to seal the bottom box part 11. And by designing the fastening cover 23 into a structure in which the main cover plate 231, triangular sealing covers 232, and positioning plate 233 cooperate, it is easy to seal the rear end face and side of the bottom box part 11 through the main cover plate 231 and triangular sealing covers 232 during use, and the stable installation and use of the liquid nitrogen bottle 81 are ensured through the setting of the positioning plate 233. And by designing the cooling component 8 into a structure in which the liquid nitrogen bottle 81 and the flow splitting nozzle 82 cooperate, the nitrogen in the liquid nitrogen bottle 81 can be sprayed into the refrigerated area from different positions during use, ensuring that the temperature in the refrigerated area is more uniform.

[0035] Refer to Figure 8 、 Figure 9 、 Figure 10 and Figure 11As shown in the figure, a guiding slide rail 221 is fixedly installed on the inner side surface of the sealed side plate 22. A horizontal bracket 222 is installed on the guiding slide rail 221. One end of the horizontal bracket 222 is slidably connected to the guiding slide rail 221, and a baffle 223 that cooperates with the picking and placing slot 10 is fixedly installed at the other end of the horizontal bracket 222. An electric cylinder 224 for driving the front end of the horizontal bracket 222 to slide is also fixedly installed on the inner side surface of the sealed side plate 22. A display screen 225 is embedded in the upper end surface of the baffle 223. By fixedly installing the guiding slide rail 221 on the inner side surface of the sealed side plate 22, it is convenient to install the horizontal bracket 222 by driving the slide rail during use. The baffle 223 is fixedly installed through the setting of the horizontal bracket 222. In this way, when the electric cylinder 224 drives the horizontal bracket 222 to move back and forth, the baffle 223 can be driven to cooperate with the picking and placing slot 10 to realize the shielding and opening of the picking and placing slot 10. At the same time, by embedding the display screen 225 on the baffle 223, it is ensured that the display screen 225 can be used to display in real time which cell storage mechanism 4 is directly below the picking and placing slot 10 at this time. By numbering different cell storage mechanisms 4, it is convenient to determine the corresponding device before opening the picking and placing slot 10, which is conducive to better precise picking and placing use.

[0036] Refer to Figure 3 and Figure 6 As shown in the figure, the moving component 3 includes a driving pulley 31, a driven pulley 32, a transmission wide belt 33, and a positioning disk 34 for installing the cell storage mechanism 4. Both the driving pulley 31 and the driven pulley 32 are rotatably installed in the refrigeration area 121, and a motor group 117 for driving the driving pulley 31 to rotate is fixedly installed on the horizontal support 113. Both ends of the transmission wide belt 33 are respectively installed on the driving pulley 31 and the driven pulley 32, and the positioning disks 34 are evenly fixed on the transmission wide belt 33. By designing the moving component 3 into a structure in which the driving pulley 31, the driven pulley 32, the transmission wide belt 33, and the positioning disk 34 cooperate with each other, it is convenient to support and install the transmission wide belt 33 by the driving pulley 31 and the driven pulley 32 during use. During use, the motor group 117 fixedly installed on the horizontal support 113 is used to drive the driving pulley 31 to rotate, thereby driving the transmission wide belt 33 to rotate stably, realizing the synchronous movement of the positioning disk 34. During the rotation process, different positioning disks 34 can be moved directly below the picking and placing slot 10, thereby realizing the movement of different cell storage mechanisms 4 into the picking and placing slot 10.

[0037] Embodiment 2 Refer to Figure 1 、 Figure 2 and Figure 3As shown in the figure, a culture test bench for cardiomyocyte hypoxia-reoxygenation experiments includes an experimental box group 1 and a sealing shell 2 fixedly installed on the rear end face of the experimental box group 1. The experimental box group 1 includes a bottom box part 11 and a top box part 12 arranged above the bottom box part 11. A partition inclined plate 13 is fixedly installed in the middle of the bottom box part 11. The partition inclined plate 13 divides the space in the bottom box part 11 into an equipment area 111 and a storage area 112. A vertical partition 14 is fixedly installed in the middle of the top box part 12. The vertical partition 14 divides the space in the top box part 12 into a refrigeration area 121 and an equipment area 122. A moving component 3 is installed in the refrigeration area 121. A plurality of cell storage mechanisms 4 are evenly installed on the upper end face of the moving component 3. A placement slot 10 corresponding to the cell storage mechanism 4 is opened on the upper end face of the experimental box group 1.

[0038] Refer to Figure 13 、 Figure 14 and Figure 15 As shown in the figure, the cell storage mechanism 4 includes a positioning ring seat 41, a placement shell 42 and a lifting component 43. The positioning ring seat 41 is fixedly installed on the upper end face of the positioning disk 34. The placement shell 42 is slidably installed in the positioning ring seat 41. The lifting component 43 is vertically installed at the center of the positioning ring seat 41, and the lifting component 43 is used to adjust the height of the placement shell 42 in the positioning ring seat 41. A test tube ring seat 44 is installed in the placement shell 42. The test tube ring seat 44 is rotatably connected to the placement shell 42. By designing the cell storage mechanism 4 into a structure in which the positioning ring seat 41, the placement shell 42 and the lifting component 43 cooperate with each other, it is easy to fixedly install it on the positioning disk 34 through the positioning ring seat 41 during use, and then install the placement shell 42 through the positioning ring seat 41 to ensure that the test tube ring seat 44 is rotatably installed through the placement shell 42. During use, the test tube can be stably placed through the test tube ring seat 44, and different use positions can be adjusted by rotation during picking and placing. During actual use, the height of the placement shell 42 can also be controlled by the lifting component 43. In this way, after the cell storage mechanism 4 rotates below the placement slot 10, the placement shell 42 can be lifted from the placement slot 10 through the lifting component 43, which is convenient for the experimenter to directly pick and place.

[0039] Refer to Figure 13 、 Figure 14 and Figure 15As shown in the figure, the positioning ring seat 41 includes a ring support plate 411, a back arc plate 412, a front arc plate 413, and an inner frame 414 for installing the lifting component 43. A set of back arc plates 412 and two sets of front arc plates 413 are evenly installed on the upper end surface of the ring support plate 411 along the circumferential direction, and both the back arc plate 412 and the front arc plate 413 are fixedly connected to the ring support plate 411. A limiting plate 415 is integrally formed on the upper end surface of the front arc plate 413. The inner frame 414 is installed in the middle of the ring support plate 411, and the inner frame 414 is integrally formed with the ring support plate 411. The placement shell 42 includes a disc plate 421 and an arc-shaped baffle 422. The arc-shaped baffle 422 is arranged on the upper end surface of the disc plate 421, and the arc-shaped baffle 422 is integrally formed with the disc plate 421. A threaded sleeve 423 is fixedly installed at the center of the disc plate 421. The lifting component 43 includes a driving motor 431 and a threaded rod 432 that cooperates with the threaded sleeve 423. The lower end of the threaded rod 432 is fixedly intercepted at the output end of the driving motor 431. By designing the positioning ring seat 41 into a structure that combines the ring support plate 411, the back arc plate 412, the front arc plate 413, and the inner frame 414, it is ensured that during use, the ring support plate 411 can be fixed to the positioning disc 34. By arranging a set of back arc plates 412 and two sets of front arc plates 413 on the ring support plate 411, the placement shell 42 can be limited by the back arc plate 412 and the front arc plate 413, ensuring that the placement shell 42 can only slide up and down for adjustment in the positioning ring seat 41. By fixedly installing the inner frame 414 in the middle of the ring support plate 411, it is ensured that the lifting component 43 can be fixedly installed through the inner frame 414 during use, facilitating the stable driving of the placement shell 42 by the lifting component 43. By arranging the limiting plate 415 on the upper end surface of the front arc plate 413, it is ensured that the lifting component 43 is limited by the limiting plate 415, preventing the lifting component 43 from rotating during the lifting process. By designing the placement shell 42 into a structure that combines the disc plate 421 and the arc-shaped baffle 422, it is easy to install the test tube ring seat 44 through the cooperation of the disc plate 421 and the arc-shaped baffle 422 during use, facilitating the stable rotational installation of the test tube ring seat 44 in the placement shell 42. By designing the lifting component 43 into a structure that combines the driving motor 431 and the threaded rod 432, it is ensured that during use, the driving motor 431 can drive the threaded rod 432 to rotate, and then drive the placement shell 42 to lift through the cooperation of the threaded rod 432 and the threaded sleeve 423.

[0040] Embodiment 3 Refer to Figure 1 、 Figure 2 and Figure 3As shown in the figure, a culture test bench for cardiomyocyte hypoxia-reoxygenation experiments includes an experimental box group 1 and a sealing shell 2 fixedly installed on the rear end face of the experimental box group 1. The experimental box group 1 includes a bottom box part 11 and a top box part 12 arranged above the bottom box part 11. A vertical partition 14 is fixedly installed in the middle of the top box part 12. The vertical partition 14 divides the space in the top box part 12 into a refrigeration area 121 and an equipment area 122. A moving component 3 is installed in the refrigeration area 121. A number of cell storage mechanisms 4 are evenly installed on the upper end face of the moving component 3. The cell storage mechanism 4 includes a positioning ring seat 41, a placement shell 42, and a lifting component 43. The positioning ring seat 41 is fixedly installed on the upper end face of the positioning disk 34. The placement shell 42 is slidably installed in the positioning ring seat 41. The lifting component 43 is vertically installed at the center of the positioning ring seat 41, and the lifting component 43 is used to adjust the height of the placement shell 42 in the positioning ring seat 41. A test tube ring seat 44 is installed in the placement shell 42, and the test tube ring seat 44 is rotatably connected to the placement shell 42.

[0041] Refer to Figure 16 As shown in the figure, the test tube ring seat 44 includes an annular seat 441, a placement arc plate 442, a vertical plate 443, a threaded push rod 444, and a placement arc plate 445. The annular seat 441 is installed on the upper end face of the disk plate 421, and the annular seat 441 is rotatably connected to the arc-shaped baffle 422. The placement arc plate 442 is fixedly installed on the upper end face of the annular seat 441. The vertical plate 443 is fixedly installed on the upper end face of the annular seat 441. The threaded push rod 444 is threadedly connected to the vertical plate 443. The placement arc plate 445 is arranged opposite to the placement arc plate 442, and the placement arc plate 445 is rotatably installed at one end of the threaded push rod 444. By designing the test tube ring seat 44 into a structure in which the annular seat 441, the placement arc plate 442, the vertical plate 443, the threaded push rod 444, and the placement arc plate 445 cooperate with each other, it is easy to rotate and install it in the arc-shaped baffle 422 through the annular seat 441 during use, and install the threaded push rod 444 through the vertical plate 443 on the annular seat 441. After rotatably installing the placement arc plate 445 at the head of the threaded push rod 444, it can cooperate with the placement arc plate 442. During use, the distance between the placement arc plate 442 and the placement arc plate 445 can be changed by rotating the threaded push rod 444, so as to facilitate the insertion and placement of test tubes of different sizes.

[0042] Example 4 Refer to Figure 1 、 Figure 2 and Figure 3As shown in the figure, a culture test bench for myocardial cell hypoxia-reoxygenation experiments includes an experimental box group 1 and a sealing shell 2 fixedly installed on the rear end face of the experimental box group 1. The experimental box group 1 includes a bottom box part 11 and a top box part 12 arranged above the bottom box part 11. A partition inclined plate 13 is fixedly installed in the middle of the bottom box part 11. The partition inclined plate 13 divides the space in the bottom box part 11 into an equipment area 111 and a storage area 112. A vertical partition plate 14 is fixedly installed in the middle of the top box part 12. The vertical partition plate 14 divides the space in the top box part 12 into a refrigeration area 121 and an equipment area 122. A moving component 3 is installed in the refrigeration area 121. A plurality of cell storage mechanisms 4 are evenly installed on the upper end face of the moving component 3. A placing and taking groove 10 corresponding to the cell storage mechanism 4 is formed on the upper end face of the experimental box group 1, and a corresponding sterilization shell 5 is arranged above the placing and taking groove 10.

[0043] Referring to Figure 12 As shown in the figure, the sterilization shell 5 includes a main shell 51 and a transparent arc cover 52 that can be slid open. The main shell 51 is fixedly installed on the upper end face of the experimental box group 1. The transparent arc cover 52 is slidably installed in the main shell 51. A sterilization lamp is fixedly installed on the inner side surface of the main shell 51. By designing the sterilization shell 5 into a structure in which the main shell 51 and the transparent arc cover 52 cooperate, the transparent arc cover 52 can be slid open during easy placing and taking operations, and can be slid closed when not in use. Moreover, before placing and taking, the main shell 51 can be sterilized by the sterilization lamp on the inner side surface of the main shell 51, which can effectively ensure the safety during the placing and taking process.

[0044] Example 5 Referring to Figure 1 、 Figure 2 and Figure 3 As shown in the figure, a culture test bench for myocardial cell hypoxia-reoxygenation experiments includes an experimental box group 1 and a sealing shell 2 fixedly installed on the rear end face of the experimental box group 1. The experimental box group 1 includes a bottom box part 11 and a top box part 12 arranged above the bottom box part 11. A partition inclined plate 13 is fixedly installed in the middle of the bottom box part 11. The partition inclined plate 13 divides the space in the bottom box part 11 into an equipment area 111 and a storage area 112. A vertical partition plate 14 is fixedly installed in the middle of the top box part 12. The vertical partition plate 14 divides the space in the top box part 12 into a refrigeration area 121 and an equipment area 122. A moving component 3 is installed in the refrigeration area 121. A plurality of cell storage mechanisms 4 are evenly installed on the upper end face of the moving component 3. A placing and taking groove 10 corresponding to the cell storage mechanism 4 is formed on the upper end face of the experimental box group 1, and a corresponding sterilization shell 5 is arranged above the placing and taking groove 10. A plurality of experimental benches 6 are also slidably installed on the upper end face of the experimental box group 1, and a driving member 7 for driving the experimental bench 6 to lift is fixedly installed in the equipment area 122.

[0045] Referring to Figure 17 、 Figure 18 、Figure 19 and Figure 20 As shown in Figure 20 , the test bench 6 includes a clean tabletop 61, a concave bracket 62, and a vertical rod bracket 63. The concave bracket 62 is fixedly installed on the lower end face of the clean tabletop 61, and the vertical rod bracket 63 is fixedly installed at both ends of the concave bracket 62. The lower end of the vertical rod bracket 63 is slidably installed in the equipment second area 122. A mating rack 631 is also fixedly installed on one side of the vertical rod bracket 63. A receiving groove 123 for receiving the concave bracket 62 is provided on the upper end face of the top box part 12. The driving member 7 includes an inner seat 71, a mounting seat 72, and a transmission shaft 73. The inner seat 71 is fixedly installed in the equipment second area 122, and both ends of the inner seat 71 are clamped on the vertical rod bracket 63. The mounting seat 72 is fixed in the middle of the inner seat 71. The transmission shaft 73 is rotatably installed on the mounting seat 72, and driving gears 731 meshing with the mating rack 631 are fixedly installed at both ends of the transmission shaft 73. A driving motor two 74 for driving the transmission shaft 73 to rotate is also fixedly installed on the lower end face of the mounting seat 72. By designing the test bench 6 into a structure in which the clean tabletop 61, the concave bracket 62, and the vertical rod bracket 63 cooperate, when in use, the clean tabletop 61 can be supported by the concave bracket 62 at the lower end, and by installing the vertical rod bracket 63 on the concave bracket 62, it is ensured that when in use, it can be inserted into the equipment second area 122 from the upper end face of the top box part 12. Without adjusting the clean tabletop 61, the vertical rod bracket 63 can be completely inserted into the top box part 12 during the experiment. When the height needs to be adjusted, the driving motor two 74 can be started to drive the transmission shaft 73 to rotate, and then the mating rack 631 can be driven to move upward by the driving gears 731 at both ends of the transmission shaft 73, so as to achieve the purpose of driving the vertical rod bracket 63 and the clean tabletop 61 to move upward synchronously, ensuring that the clean tabletop 61 can be stably raised for use. By providing a receiving groove 123 for receiving the concave bracket 62 on the upper end face of the top box part 12, the concave bracket 62 can be received into the receiving groove 123 when it is not necessary to raise it for use, facilitating the stable use of the clean tabletop 61 by fitting it on the upper end face of the top box part 12.

[0046] Working principle: When the experimental bench 6 is in use, first, the experimenter can start the drive motor two 74 according to personal needs to drive the transmission shaft 73 to rotate. Through the cooperation of the drive gears 731 at both ends of the transmission shaft 73 and the mating rack 631, the vertical rod frame 63 is driven to rise synchronously, thereby facilitating the change of the use height of the experimental bench 6. During the myocardial cell hypoxia-reoxygenation experiment, the experimenter can start the motor group 117 to drive the drive pulley 31 to rotate, so as to adjust different cell storage mechanisms 4 to move to the position of the access slot 10. Then, after opening the cover 223, the placement shell 42 can be lifted from the position of the access slot 10 through the lifting component 43. In this way, after the experimenter slides open the transparent arc cover 52, the items can be directly taken and placed. Different cell storage mechanisms 4 facilitate the experimenter to take and place different items, and the items placed in the refrigeration area 121 can be refrigerated through the cooling component 8, effectively improving the efficiency and safety of the experimenter's access.

[0047] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various modifications and changes. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A culture test bench for myocardial cell hypoxia and reoxygenation experiments, comprising an experimental box set (1) and a sealing shell (2) fixedly mounted on the rear end surface of the experimental box set (1), characterized in that: The experimental box set (1) comprises a bottom box part (11) and a top box part (12) arranged above the bottom box part (11); a partition slant plate (13) is fixedly installed in the middle of the bottom box part (11); the partition slant plate (13) divides the space in the bottom box part (11) into a first equipment area (111) and a storage area (112); a vertical partition plate (14) is fixedly installed in the middle of the top box part (12); the vertical partition plate (14) divides the space in the top box part (12) into a refrigerated area (121) and a second equipment area (122); a movable partition plate (14) is installed in the refrigerated area (121) The upper end surface of the movable component (3) is evenly mounted with a plurality of cell storage mechanisms (4); the upper end surface of the experimental box group (1) is provided with a pick-up and release groove (10) corresponding to the cell storage mechanism (4), and a corresponding sterilization shell (5) is arranged above the pick-up and release groove (10); the upper end surface of the experimental box group (1) is also slidably mounted with a plurality of experimental tables (6), and a driving member (7) for driving the experimental tables (6) to rise and fall is fixedly mounted in the second equipment area (122); and a cooling component (8) for cooling the refrigerated area (121) is fixedly mounted on the sealed shell (2).

2. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 1, characterized in that: A horizontal support (113) is installed in the equipment zone (111), and the horizontal support (113) is fixedly connected to the equipment zone (111). A plurality of partition plates (114) are installed in the storage zone (112), and the partition plates (114) divide the storage zone (112) into a plurality of different storage spaces. A test box door (115) corresponding to each storage space is installed on the front end surface of the storage zone (112), and a plurality of equipment legs (116) are evenly installed on the lower end surface of the storage zone (112).

3. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 2, characterized in that: The sealed housing (2) comprises a back cover (21), a sealing side plate (22), and a buckle cover (23) for sealing the first device zone (111); the sealing side plates (22) are mounted on both sides of the back cover (21), and the sealing side plates (22) and the back cover (21) are integrally formed; the buckle cover (23) comprises a main cover plate (231), a triangular sealing cover (232), and a positioning plate (233) for mounting a cooling component (8); the main cover plate (231) is fixedly mounted on the front end surface of the back cover (21); the triangular sealing cover (232) is 2) are arranged on both sides of the main cover plate (231), the positioning plate (233) is installed on the front end surface of the main cover plate (231), and the main cover plate (231), the triangular cover (232) and the positioning plate (233) are integrally formed, the cooling component (8) comprises a liquid nitrogen bottle (81) and a diverter nozzle (82), the liquid nitrogen bottle (81) is fixedly installed on the positioning plate (233), the diverter nozzle (82) is fixedly installed on the sealing side plate (22), and the diverter nozzle (82) is connected to the gas outlet of the liquid nitrogen bottle (81).

4. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 3, characterized in that: A guide rail (221) is fixedly mounted on the inner side surface of the sealing side plate (22), a horizontal bracket (222) is mounted on the guide rail (221), one end of the horizontal bracket (222) is slidably connected to the guide rail (221), and a blocking cover (223) matching the pick-and-place groove (10) is fixedly mounted on the other end of the horizontal bracket (222), an electric cylinder (224) for driving the front end of the horizontal bracket (222) to slide is also fixedly mounted on the inner side surface of the sealing side plate (22), and a display screen (225) is embedded in the upper end surface of the blocking cover (223).

5. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 4, characterized in that: The moving assembly (3) comprises a driving pulley (31), a driven pulley (32), a transmission broadband (33), and a positioning plate (34) for mounting a cell storage mechanism (4); the driving pulley (31) and the driven pulley (32) are both rotatably mounted in the cold storage area (121); a motor group (117) for driving the driving pulley (31) to rotate is fixedly mounted on the horizontal bracket (113); two ends of the transmission broadband (33) are respectively mounted on the driving pulley (31) and the driven pulley (32); and the positioning plate (34) is evenly fixed on the transmission broadband (33).

6. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 5, characterized in that: The cell storage mechanism (4) comprises a positioning ring seat (41), a placement shell (42) and a lifting assembly (43); the positioning ring seat (41) is fixedly mounted on the upper end surface of the positioning plate (34); the placement shell (42) is slidably mounted in the positioning ring seat (41); the lifting assembly (43) is vertically mounted at the center of the positioning ring seat (41); and the lifting assembly (43) is used to adjust the height of the placement shell (42) in the positioning ring seat (41); a test tube ring seat (44) is mounted in the placement shell (42); and the test tube ring seat (44) is rotatably connected to the placement shell (42).

7. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 6, characterized in that: The positioning ring seat (41) comprises a ring frame plate (411), a back arc plate (412), a front arc plate (413), and an inner frame (414) for mounting the lifting assembly (43); a group of back arc plates (412) and two groups of front arc plates (413) are evenly mounted on the upper end surface of the ring frame plate (411) along the circumferential direction, and the back arc plates (412) and the front arc plates (413) are fixedly connected to the ring frame plate (411); the upper end surface of the front arc plate (413) is also integrally formed with a limiting plate (415); the inner frame (414) is mounted in the middle of the ring frame plate (411), and the inner frame (414) is connected to the ring frame plate (411). The ring frame plate (411) is integrally formed, the placement shell (42) comprises a disc plate (421) and an arc-shaped baffle plate (422), the arc-shaped baffle plate (422) is arranged on the upper end surface of the disc plate (421), and the arc-shaped baffle plate (422) and the disc plate (421) are integrally formed, a threaded sleeve (423) is fixedly installed at the center of the disc plate (421), and the lifting component (43) comprises a driving motor (431) and a threaded rod (432) matched with the threaded sleeve (423), and the lower end of the threaded rod (432) is fixedly intercepted at the output end of the driving motor (431).

8. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 7, characterized in that: The test tube ring seat (44) comprises an annular seat (441), a placement arc plate 1 (442), a vertical plate (443), a threaded push rod (444) and a placement arc plate 2 (445); the annular seat (441) is mounted on the upper end surface of the disc plate (421), and the annular seat (441) is rotatably connected to the arc baffle (422); the placement arc plate 1 (442) is fixedly mounted on the upper end surface of the annular seat (441); the vertical plate (443) is fixedly mounted on the upper end surface of the annular seat (441); the threaded push rod (444) is threadedly connected to the vertical plate (443); the placement arc plate 2 (445) is arranged opposite to the placement arc plate 1 (442), and the placement arc plate 2 (445) is rotatably mounted on one end of the threaded push rod (444).

9. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 8, characterized in that: The sterilization shell (5) comprises a main shell (51) and a transparent arc cover (52) that can be slid open. The main shell (51) is fixedly mounted on the upper end surface of the experimental box group (1), and the transparent arc cover (52) is slidably mounted in the main shell (51). A sterilization lamp is fixedly mounted on the inner side surface of the main shell (51).

10. A culture test bench for myocardial cell hypoxia and reoxygenation experiments according to claim 9, characterized in that: The laboratory bench (6) comprises a clean bench top (61), a concave bracket (62) and a vertical rod frame (63), wherein the concave bracket (62) is fixedly mounted on the lower end surface of the clean bench top (61), the vertical rod frame (63) is fixedly mounted on both ends of the concave bracket (62), and the lower end of the vertical rod frame (63) is slidably mounted on the second equipment zone (122), and a matching rack (631) is also fixedly mounted on one side of the vertical rod frame (63), and a storage groove (123) for accommodating the concave bracket (62) is provided on the upper end surface of the top box portion (12), and the driving member (7) comprises an inner The invention relates to a base (71), a mounting base (72) and a transmission shaft (73), wherein the inner base (71) is fixedly mounted on the second equipment zone (122), and the two ends of the inner base (71) are clamped on the vertical rod frame (63), the mounting base (72) is fixed on the middle part of the inner base (71), the transmission shaft (73) is rotatably mounted on the mounting base (72), and the two ends of the transmission shaft (73) are fixedly mounted with driving gears (731) meshing with the matching rack (631), and the lower end surface of the mounting base (72) is also fixedly mounted with a second driving motor (74) for driving the transmission shaft (73) to rotate.

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