Application of molybdenum disulfide nano material in crop cold damage regulation and control

Abstract

The invention discloses application of a molybdenum disulfide nano material in crop cold damage regulation and control, and belongs to the technical field of anti-cold agents. The preparation method comprises the following steps: dispersing a molybdenum disulfide nano material in water to obtain a suspension; then the suspension is sprayed on corn and soybean leaves, the cold resistance of the corn and the soybean is regulated and controlled in a leaf surface spraying mode, the biomass of the cold-damaged corn and the soybean can be increased, and the application prospect is excellent.

Description

Technical Field

[0001] The present invention belongs to the technical field of novel crop antifreeze agents, and particularly relates to the application of molybdenum disulfide nanomaterials in crop chilling injury regulation. Background Art

[0002] With the continuous increase in the global population, the demand for food has gradually increased. However, low-temperature freezing disasters seriously threaten the global food production safety, and the occurrence frequency and influence range show an increasing trend year by year. Reported cold-resistant reagents include abscisic acid (ABA), salicylic acid (SA), betaine, polyamines, calcium ions, ethephon, proline, chitosan, spermidine, sodium nitroprusside (nitric oxide donor), etc. These substances have applications in plant stress resistance, especially in cold resistance. However, high concentrations of abscisic acid may inhibit plant growth, and it has high costs and is easily affected by environmental degradation; excessive use of salicylic acid may cause oxidative damage, and the effect depends on the application concentration and environmental conditions; betaine has limited effects on some crops and multiple applications are required to increase costs; polyamines (such as putrescine) are easily decomposed at high or low temperatures, and excessive amounts lead to cytotoxicity; calcium ions (Ca 2+ ) need to have an antagonistic effect with other elements, and the concentration needs to be precisely regulated; ethephon (ETH) may accelerate senescence; it has phytotoxicity to sensitive crops (such as tomatoes); the exogenous application efficiency of proline is low, and it needs to be used in combination with other stress-resistant substances synergistically; the molecular weight of chitosan affects the effect and may change the soil microbial community; spermidine has high costs and poor stability and requires special storage conditions; sodium nitroprusside produces toxicity in excess and is easily decomposed under light and needs to be stored away from light.

[0003] Currently, in order to enhance the cold resistance of crops, traditional plant antifreeze agents (containing substances such as amino acids, Cu, Fe, Mn, Zn, B, and Mo) are widely used. However, the excessive application and improper application methods of plant antifreeze agents will cause phytotoxicity to crops, inhibit the normal growth of crops, and damage environmental health. Therefore, there is an urgent need to develop a safe and efficient crop cold-resistant agent to enhance the cold resistance of crops. Summary of the Invention

[0004] [Technical Problem]

[0005] Provide a method for regulating crop chilling injury that is effectively antifreeze and cold-resistant, has no phytotoxicity, and promotes crop growth.

[0006] [Technical Solution]

[0007] The present invention provides an application of molybdenum disulfide nanomaterials in crop chilling injury regulation.

[0008] The present invention also provides an application of molybdenum disulfide nanomaterials in the preparation of crop antifreeze agents.

[0009] In one embodiment of the present invention, the crop antifreeze also includes any one or more of the following: inorganic salts, saccharide substances, organic alcohol substances, etc.

[0010] In one embodiment of the present invention, the inorganic salts include any one or more of the following: potassium dihydrogen phosphate, potassium nitrate.

[0011] In one embodiment of the present invention, the saccharide substances include any one or more of the following: glucose, sucrose, trehalose.

[0012] In one embodiment of the present invention, the organic alcohol substances include any one or more of the following: propylene glycol, ethylene glycol.

[0013] In one embodiment of the present invention, molybdenum disulfide nanomaterials (MoS 2 NMs) are dispersed in water to obtain a suspension; then the suspension is sprayed on the leaves of the crops.

[0014] In one embodiment of the present invention, the crops are vegetables. Specifically, it includes green leafy vegetables such as lettuce, spinach, coriander, and green vegetables.

[0015] In one embodiment of the present invention, the plants are crops, including corn, soybean, rice, etc.

[0016] In one embodiment of the present invention, the concentration of the suspension is 20 - 200 mg / L. Preferably 200 mg / L.

[0017] In one embodiment of the present invention, MoS 2 NMs have a flaky structure with an average size of 173.82 ± 15.20 nm.

[0018] In one embodiment of the present invention, the MoS 2 NMs are prepared by the following method:

[0019] The molybdenum source and the sulfur source are respectively dissolved in water to prepare a molybdenum source solution and a sulfur source solution; the two are mixed evenly and transferred to a hydrothermal reaction kettle for hydrothermal reaction. After the reaction is completed, centrifugation, collection of the precipitate, washing, and drying are carried out to obtain MoS 2 NMs.

[0020] In one embodiment of the present invention, the molybdenum source is sodium molybdate or its hydrate. Specifically, Na 2 MoO 4 ·2H 2 O can be selected.

[0021] In one embodiment of the present invention, the sulfur source is L-cysteine.

[0022] In one embodiment of the present invention, the mass ratio of the molybdenum source to the sulfur source is (3 - 4):4.

[0023] In one embodiment of the present invention, the pH of the molybdenum source solution is 6.5 and the concentration is 5 - 8 mg / mL.

[0024] In one embodiment of the present invention, the concentration of the sulfur source solution is 5 - 8 mg / mL.

[0025] In one embodiment of the present invention, the temperature of the hydrothermal reaction is 180 - 240 °C and the time is 10 - 20 h. Specifically, it can be 200 °C for 15 h.

[0026] In one embodiment of the present invention, the preparation method of the MoS 2 NMs specifically includes:

[0027] Dissolve Na 2 MoO 4 ·2H 2 O in ultrapure water and adjust the pH to 6.5 with HCl, and mix well. At the same time, dissolve L-cysteine in ultrapure water. After complete dissolution, mix the above two solutions and mix well. Then transfer the mixed solution to a stainless steel hydrothermal reaction kettle and react at 200 °C for 15 h. After the reaction, centrifuge, collect the precipitate, wash, and dry to obtain MoS 2 NMs.

[0028] The main content of the present invention includes:

[0029] (1) Prepared molybdenum disulfide nanomaterials (MoS 2 NMs);

[0030] (2) By means of foliar spraying, apply MoS 2 NMs with different concentrations to corn and soybeans to explore the effect of enhancing the cold resistance of corn and soybeans;

[0031] (3) By means of foliar spraying, apply the optimal concentration of MoS 2 NMs, MoS 2 large particles (MoS 2 BPs), sulfur-equivalent sulfates (Na 2 SO 4 ), molybdenum-equivalent molybdates (Na 2 MoO 4 . 2H 2 O), and traditional plant antifreeze agents at agricultural guidance concentrations to corn and soybeans to explore the effect of enhancing the cold resistance of corn and soybeans.

[0032] [Beneficial effects]

[0033] The present invention disperses molybdenum disulfide nanomaterials in water to prepare a suspension, and applies it to corn and soybean plants by foliar spraying. This method effectively enhances the cold resistance of corn and soybeans, and significantly increases the biomass of seedlings suffering from chilling injury. Among them, the above-ground biomass increases by 27.4% and 20.5% respectively, and the below-ground biomass increases by 30.4% and 41.9% respectively, showing broad application prospects. The effects of different materials in enhancing the cold resistance of corn and soybeans are as follows: MoS 2 NMs > Plant antifreeze agent > Na 2 SO 4 ≈Na 2 MoO 4 ≈Na 2 SO 4 +Na 2 MoO 4 . 2H 2 O > MoS 2 BPs. Description of the drawings

[0034] Figure 1 (A) TEM image of MoS 2 NMs (scale bar is 100 nm); (B) TEM photograph of MoS 2 NMs (scale bar is 20 nm); (C) XRD pattern of MoS 2 NMs.

[0035] Figure 2 Effects of foliar application of different concentrations of MoS 2 NMs on (A) above-ground and below-ground biomass of corn, (B) plant height of corn, (C) above-ground and below-ground biomass of soybeans, and (D) plant height of soybeans. S20, S50, S100, and S200 in the figure respectively refer to the application examples of foliar application of 20 mg / L, 50 mg / L, 100 mg / L, and 200 mg / L of MoS 2 NMs in Example 4.

[0036] Figure 3 Effects of foliar application of 200 mg / L MoS 2 NMs, 200 mg / L MoS 2 BPs, 355 mg / L Na 2 SO 4 , 302 mg / L Na 2 MoO 4 , 355 mg / L Na 2 SO 4 + 302 mg / L Na 2MoO 4 The effects of 1667 mg / L of plant antifreeze agent on (A) the aboveground and underground biomass of corn and (B) the aboveground and underground biomass of soybean. Detailed implementation manners

[0037] The present invention will be further described below in conjunction with specific embodiments.

[0038] The embodiments provided below are not intended to limit the scope covered by the present invention, and the described steps are not intended to limit their execution order. Obvious improvements made by those skilled in the art to the present invention in combination with the existing well-known common sense also fall within the protection scope required by the present invention.

[0039] Example 1 Preparation of molybdenum disulfide nanomaterials

[0040] Dissolve 0.35 g of Na 2 MoO 4 ·2H 2 O in 60 mL of ultrapure water, and adjust the pH to 6.5 using 1 mol / L HCl, and stir for 10 minutes. Meanwhile, dissolve 0.4 g of L-cysteine in 80 mL of ultrapure water. After complete dissolution, mix the above two solutions and stir for 40 minutes. Subsequently, transfer the mixed solution to a stainless steel hydrothermal reaction kettle with a 200 mL polytetrafluoroethylene inner liner, and react at 200 °C for 15 hours. After the reaction is completed, centrifuge to collect the black precipitate, and wash it alternately with deionized water and absolute ethanol for multiple times, and finally dry it at 80 °C for 12 hours.

[0041] Material characterization:

[0042] Use a transmission electron microscope (TEM) to characterize the morphology and size of MoS 2 NMs. The results show that MoS 2 NMs exhibit a typical flake structure, and the average size is 173.82 ± 15.20 nm ( Figure 1 A, B). In addition, the characteristic peaks at 9.2°, 18.0°, 32.8° and 57.2° in the XRD spectrum of MoS 2 NMs correspond to the lattice planes 001, 002, 100 and 110 of MoS 2 respectively ( Figure 1 C).

[0043] Example 2 Preparation of molybdenum disulfide nanomaterials

[0044] Dissolve 0.35 g of Na 2 MoO 4 ·2H 2O was dissolved in 60 mL of ultrapure water, and the pH was adjusted to 6.5 using 1 mol / L HCl, followed by stirring for 10 minutes. Meanwhile, 0.4 g of L-cysteine was dissolved in 80 mL of ultrapure water. After complete dissolution, the above two solutions were mixed and stirred for 40 minutes. Subsequently, the mixed solution was transferred to a 200 mL stainless steel hydrothermal reactor with a Teflon liner and reacted at 180 °C for 20 hours. After the reaction, the black precipitate was collected by centrifugation, washed alternately with deionized water and absolute ethanol for several times, and finally dried at 80 °C for 12 hours.

[0045] Example 3 Preparation of Molybdenum Disulfide Nanomaterials

[0046] Dissolve 0.35 g of Na 2 MoO 4 ·2H 2 O in 60 mL of ultrapure water, and adjust the pH to 6.5 using 1 mol / L HCl, then stir for 10 minutes. At the same time, dissolve 0.4 g of L-cysteine in 80 mL of ultrapure water. After complete dissolution, mix the above two solutions and stir for 40 minutes. Then transfer the mixed solution to a 200 mL stainless steel hydrothermal reactor with a Teflon liner and react at 240 °C for 10 hours. After the reaction, collect the black precipitate by centrifugation, wash it alternately with deionized water and absolute ethanol for several times, and finally dry it at 80 °C for 12 hours.

[0047] Example 4 Preparation of Molybdenum Disulfide Nanomaterials

[0048] Dissolve 0.3 g of Na 2 MoO 4 ·2H 2 O in 60 mL of ultrapure water, and adjust the pH to 6.5 using 1 mol / L HCl, then stir for 10 minutes. At the same time, dissolve 0.4 g of L-cysteine in 80 mL of ultrapure water. After complete dissolution, mix the above two solutions and stir for 40 minutes. Then transfer the mixed solution to a 200 mL stainless steel hydrothermal reactor with a Teflon liner and react at 200 °C for 15 hours. After the reaction, collect the black precipitate by centrifugation, wash it alternately with deionized water and absolute ethanol for several times, and finally dry it at 80 °C for 12 hours.

[0049] Example 5 Preparation of Molybdenum Disulfide Nanomaterials

[0050] Dissolve 0.35 g of Na 2 MoO 4 ·2H 2O was dissolved in 50 mL of ultrapure water, and the pH was adjusted to 6.5 using 1 mol / L HCl, followed by stirring for 10 minutes. Meanwhile, 0.4 g of L-cysteine was dissolved in 80 mL of ultrapure water. After complete dissolution, the above two solutions were mixed and stirred for 40 minutes. Subsequently, the mixed solution was transferred to a 200 mL stainless-steel hydrothermal reaction kettle with a polytetrafluoroethylene inner liner and reacted at 200 °C for 15 hours. After the reaction, the black precipitate was collected by centrifugation and washed alternately with deionized water and absolute ethanol multiple times, and finally dried at 80 °C for 12 hours.

[0051] Example 6 Preparation of molybdenum disulfide nanomaterials

[0052] Dissolve 0.35 g of Na 2 MoO 4 ·2H 2 O in 60 mL of ultrapure water, and adjust the pH to 6.5 using 1 mol / L HCl, followed by stirring for 10 minutes. Meanwhile, 0.4 g of L-cysteine was dissolved in 50 mL of ultrapure water. After complete dissolution, the above two solutions were mixed and stirred for 40 minutes. Subsequently, the mixed solution was transferred to a 200 mL stainless-steel hydrothermal reaction kettle with a polytetrafluoroethylene inner liner and reacted at 200 °C for 15 hours. After the reaction, the black precipitate was collected by centrifugation and washed alternately with deionized water and absolute ethanol multiple times, and finally dried at 80 °C for 12 hours.

[0053] Example 7 Regulation of cold resistance of corn and soybean using molybdenum disulfide nanomaterials with different concentrations

[0054] Disperse the MoS 2 NMs obtained in Example 1 in water to prepare MoS 2 NMs suspensions with concentrations of 20, 50, 100, and 200 mg / L.

[0055] Use corn (Zea mays L.) and soybean (Glycine max L.) as the test crops. Disinfect the seeds of corn (Dafeng 30) and soybean (Zhonghuang 57) with 5% sodium hypochlorite solution for 5 min, and then rinse them with deionized water multiple times. After soaking the disinfected corn and soybean seeds in deionized water for 6 h and 3 h respectively, place them on a seedling tray with wet filter paper. Germinate in the dark at a temperature of 25 °C until the heights of the corn and soybean seedlings reach about 3 cm. Select corn and soybean seedlings with consistent growth and transplant them into flower pots containing 600 g of soil. When the corn grows to three leaves and one core, and the two pairs of compound leaves of the soybean are fully developed, apply 5 mL of MoS 2The MoS₂ NMs suspension was sprayed on the leaves of maize and soybean for 5 consecutive days. The healthy control group (Healthy) and the chilling injury control group (Cold) were sprayed with an equal volume of deionized water. Two days later, the maize and soybean were placed in a light incubator for low temperature stress, with a day / night temperature of 10 / 8 °C, a light / dark cycle of 14 / 10 h, a relative humidity of 60% ± 5%, and a light intensity of 16800 Lux. The day / night temperature of the light incubator for the healthy control group was 25 / 20 °C, and the other conditions remained the same. After 1 week, the photosynthetic parameters, chlorophyll fluorescence parameters, and relative chlorophyll content of the maize and soybean leaves were measured. Subsequently, destructive sampling was carried out, and the fresh weight of the crops was recorded.

[0056] The results showed that foliar application of 200 mg / L MoS₂ 2 NMs significantly increased the aboveground fresh weight (27.4%, 20.5%), underground fresh weight (47.1%, 41.9%), and plant height (33.3%, 19.8%) of chilling-injured maize and soybean seedlings, and the promotion effect was better than that of 20, 50, and 100 mg / L MoS₂ 2 NMs ( Figure 2 A-D). Therefore, the optimal application concentration of MoS₂ 2 NMs for enhancing the cold resistance of maize and soybean was 200 mg / L. The specific results are shown in Table 1.

[0057] Table 1 Results of enhancing the cold resistance of maize and soybean by MoS₂ 2 NMs

[0058]

[0059]

[0060] Example 8 Application of molybdenum disulfide nanomaterials to regulate the cold resistance of maize and soybean

[0061] The MoS₂ 2 NMs obtained in Example 1 were dispersed in water to prepare a 200 mg / L MoS₂ 2 NMs suspension.

[0062] 200 mg / L MoS₂ 2 NMs, 200 mg / L MoS₂ 2 large particles (MoS₂ 2 BPs), 355 mg / L Na₂ 2 SO₄ ( 4 equal in S amount to 200 mg / L MoS₂ 2 NMs), 302 mg / L Na₂ 2 MoO₄ 4. 2H 2 O (equivalent to 200 mg / L MoS 2 NMs), 355 mg / L Na 2 SO 4 + 302 mg / L Na 2 MoO 4 . 2H 2 O and 1667 mg / L commercially available plant antifreeze (commercial recommended concentration) were applied to the leaves of corn and soybean, and the experimental process was the same as that in Example 7.

[0063] Among them, MoS 2 large particles (MoS 2 BPs) had a purity of GR, CAS of 1317 - 33 - 5, and could be purchased from Beijing Innochem Science & Technology Co., Ltd.

[0064] The results showed that foliar spraying of MoS 2 NMs significantly increased the aboveground biomass and underground biomass of chilling - injured corn, which was better than the equivalent amount of MoS 2 BPs, Na 2 SO 4 , Na 2 MoO 4 , Na 2 SO 4 + Na 2 MoO 4 and plant antifreeze ( Figure 3 A); foliar spraying of MoS 2 NMs significantly increased the aboveground biomass and underground biomass of chilling - injured soybean, which was better than the equivalent amount of MoS 2 BPs, Na 2 SO 4 , Na 2 MoO 4 , Na 2 SO 4 + Na 2 MoO 4 and plant antifreeze ( Figure 3 B); indicating that MoS 2 NMs had the best effect on enhancing the cold resistance of corn and soybean, and was significantly better than the enhancement effect of traditional plant antifreeze. The specific results are shown in Table 2.

[0065] Table 2 Results of different cold - resistant agents regulating the cold resistance of corn and soybean

[0066]

[0067] Example 9 Application of molybdenum disulfide nanomaterials to regulate the cold resistance of corn and soybean

[0068] Disperse the MoS 2 NMs obtained in Example 1 in water to prepare a 20 mg / L MoS 2 NMs suspension; then apply it to the leaves of corn and soybean, and the experimental process is the same as that in Example 7.

[0069] The results show that MoS 2 NMs has the effect of enhancing the cold resistance of rice.

[0070] Example 10 Application of Molybdenum Disulfide Nanomaterials to Regulate the Cold Resistance of Corn and Soybean

[0071] Disperse the MoS 2 NMs obtained in Example 1 in water to prepare a 50 mg / L MoS 2 NMs suspension; then apply it to the leaves of corn and soybean, and the experimental process is the same as that in Example 7.

[0072] The results show that MoS 2 NMs has the effect of enhancing the cold resistance of rice.

[0073] Example 11 Application of Molybdenum Disulfide Nanomaterials to Regulate the Cold Resistance of Corn and Soybean

[0074] Disperse the MoS 2 NMs obtained in Example 1 in water to prepare a 100 mg / L MoS 2 NMs suspension; then apply it to the leaves of corn and soybean, and the experimental process is the same as that in Example 7.

[0075] The results show that MoS 2 NMs has the effect of enhancing the cold resistance of rice.

[0076] Example 12 Application of Molybdenum Disulfide Nanomaterials to Regulate the Cold Resistance of Rice

[0077] Disperse the MoS 2 NMs obtained in Example 1 in water to prepare a 200 mg / L MoS 2 NMs suspension; then apply it to the leaves of rice, and the experimental process is the same as that in Example 7.

[0078] The results show that MoS 2 NMs has the effect of enhancing the cold resistance of rice.

[0079] Example 13 Application of Molybdenum Disulfide Nanomaterials to Regulate the Cold Resistance of Vegetables

[0080] Disperse the MoS 2The NMs were dispersed in water to prepare a 200 mg / L MoS 2 NM suspension; then it was applied to the leaves of vegetables (green leafy vegetables such as lettuce, spinach, coriander, and green vegetables), and the experimental process was the same as that in Example 7.

[0081] The results showed that MoS 2 NMs had the effect of enhancing the cold resistance of various vegetables.

[0082] Example 14 Application of thermosensitive regulation molybdenum disulfide composite nanomaterials in the preparation of crop antifreeze agents

[0083] A crop antifreeze agent contains the following components: the thermosensitive regulation molybdenum disulfide composite nanomaterials obtained in Example 1 and inorganic salts. Among them: the inorganic salt is potassium dihydrogen phosphate.

[0084] Example 15 Application of thermosensitive regulation molybdenum disulfide composite nanomaterials in the preparation of crop antifreeze agents

[0085] A crop antifreeze agent contains the following components: the thermosensitive regulation molybdenum disulfide composite nanomaterials obtained in Example 1 and saccharide substances. Among them: the saccharide substances are glucose and sucrose.

[0086] Example 16 Application of thermosensitive regulation molybdenum disulfide composite nanomaterials in the preparation of crop antifreeze agents

[0087] A crop antifreeze agent contains the following components: the thermosensitive regulation molybdenum disulfide composite nanomaterials obtained in Example 1, inorganic salts, and saccharide substances. Among them: the inorganic salt is potassium nitrate, and the saccharide substances are glucose and sucrose.

[0088] Example 17 Application of thermosensitive regulation molybdenum disulfide composite nanomaterials in the preparation of crop antifreeze agents

[0089] A crop antifreeze agent contains the following components: the thermosensitive regulation molybdenum disulfide composite nanomaterials obtained in Example 1, inorganic salts, saccharide substances, and organic alcohol substances. Among them: the inorganic salt is potassium dihydrogen phosphate, the saccharide substance is trehalose, and the organic alcohol substance is propylene glycol.

[0090] Example 18 Application of thermosensitive regulation molybdenum disulfide composite nanomaterials in the preparation of crop antifreeze agents

[0091] A crop antifreeze agent contains the following components: the thermosensitive regulation molybdenum disulfide composite nanomaterials obtained in Example 1, inorganic salts, saccharide substances, and organic alcohol substances. Among them: the inorganic salt is potassium nitrate, the saccharide substances are glucose and sucrose, and the organic alcohol substance is ethylene glycol.

[0092] The embodiments provided above are not intended to limit the scope covered by the present invention, nor are the described steps intended to limit the order of their execution. Obvious improvements made by those skilled in the art in combination with the existing well-known general knowledge also fall within the protection scope defined by the claims of the present invention.

Claims

1. Application of a molybdenum disulfide nanomaterial in regulating crop chilling injury.

2. A molybdenum disulfide nanomaterial prepared and used in crop antifreeze.

3. The use according to claim 1 or 2, characterized in that: MoS2 nanomaterials have a flake structure with an average size of 173.82±15.20nm.

4. The use according to claim 1, characterized in that: The method is to disperse molybdenum disulfide nanomaterials in water to obtain a suspension; then the suspension is sprayed on the leaves of crops.

5. The use according to claim 4, characterized in that: The concentration of the suspension is 20-200 mg / L, preferably 200 mg / L.

6. The use according to claim 1 or 2, characterized in that: The molybdenum disulfide nanomaterial is prepared by the following method: The molybdenum source and the sulfur source are respectively dissolved in water to prepare a molybdenum source solution and a sulfur source solution; the two are mixed and transferred to a hydrothermal reactor for hydrothermal reaction. After the reaction is completed, centrifugation is performed, and the precipitate is collected, washed, and dried to obtain a molybdenum disulfide nanomaterial.

7. The use according to claim 6, characterized in that: The molybdenum source is sodium molybdate or its hydrate, and the sulfur source is L-cysteine.

8. The use according to claim 6, characterized in that: The mass ratio of the molybdenum source to the sulfur source is (3-4):

4.

9. The use according to claim 6, characterized in that: The pH of the molybdenum source solution is 6.5, and the concentration is 5-8 mg / mL.

10. The use according to claim 6, characterized in that: The concentration of the sulfur source solution is 5-8 mg / mL.

11. The use according to claim 6, characterized in that: The temperature of the hydrothermal reaction is 180-240°C and the time is 10-20h.

12. The use according to claim 2, characterized in that: The plant antifreeze agent also includes any one or more of the following: inorganic salts, sugar substances, and organic alcohol substances.

13. The use according to claim 12, characterized in that: The inorganic salt includes any one or more of the following: potassium dihydrogen phosphate, potassium nitrate.

14. The use according to claim 12, characterized in that: The carbohydrate substances include any one or more of the following: glucose, sucrose, and trehalose.

15. The use according to claim 12, characterized in that: The organic alcohol substances include any one or more of the following: propylene glycol, ethylene glycol.

16. The use according to claim 1, characterized in that: The plants are crops, including corn, soybeans and rice.

17. The use according to claim 1, characterized in that: The plants are vegetables.

18. The use according to claim 17, characterized in that The vegetables include lettuce, spinach, coriander and green vegetables.

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