Automatic monitoring system for insect anesthesia

Abstract

The invention discloses an automatic monitoring system for insect anesthesia. The automatic monitoring system comprises a test container, an anesthetic gas monitoring device, a counter and a recovery container. The test container is provided with a containing cavity, the top of the containing cavity is provided with an air inlet and a placing opening, the bottom of the containing cavity is provided with a recovery opening, the air inlet is used for being connected with an external anesthetic gas source, the placing opening is used for placing experimental insects, the containing cavity is further provided with a plurality of blocking pieces which are vertically distributed at intervals, and each blocking piece is provided with a guide face which is obliquely arranged. The anesthetic gas monitoring device is used for testing the anesthetic gas concentration at the bottom of the containing cavity, and the counter is installed below the containing cavity. The recycling container is arranged below the recycling opening and used for recycling experimental insects. The automatic monitoring device for insect anesthesia can conveniently count insects, can better distinguish sleeping insects from anesthetized insects, can realize efficient insect anesthesia, meets experimental requirements of sensitivity screening of anesthesia of different genotypes and the like, shortens operation time, and is easy to observe.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technologies, and particularly to an automatic insect anesthesia monitoring system. Background Art

[0002] Insects such as Drosophila melanogaster occupy an irreplaceable position in multiple fields such as genetics, developmental biology, biochemistry, and molecular biology due to their clear genetic background, simple nervous system, delicate information system, and complex behaviors. With the increase in the number of people undergoing major surgeries globally, general anesthesia is indispensable in clinical medicine. General anesthesia can induce reversible loss and recovery of brain consciousness, and its mechanism is one of the most intractable problems in behavioral science and pharmacology. Insects such as Drosophila melanogaster have been proven to be excellent animal models for anesthesia research because gene manipulation can be easily queried and detected at the anesthesia endpoint. It can not only solve the problems of neurotransmitter single molecule and quantum release in larval synapses, but also perform electrophysiology or calcium imaging at the circuit level or whole-brain level under anesthesia.

[0003] When performing anesthesia monitoring, due to factors such as the small size of Drosophila melanogaster, large number of samples, and numerous experimental groups, as well as reasons of the operator, problems such as large data differences and low efficiency are likely to occur. Conventional insect anesthesia monitoring operations are relatively cumbersome, with difficult counting and hard-to-distinguish anesthesia states. Summary of the Invention

[0004] The purpose of the present invention is to propose an automatic insect anesthesia monitoring device that can conveniently count insects, can better distinguish sleeping and anesthetized insects for easy observation, so as to meet experimental requirements such as screening of anesthesia sensitivity of different genotypes.

[0005] To achieve the above technical effects, the technical solution of the present invention is as follows:

[0006] The present invention discloses an automatic insect anesthesia monitoring system, including: a test container having a receiving cavity, the top of the receiving cavity having an air inlet and a placing opening, and the bottom having a recovery opening. The air inlet is used to connect to an external anesthesia gas source, the placing opening is used to place experimental insects, and the receiving cavity is further provided with a plurality of blocking members vertically spaced apart. Each blocking member has an inclined guiding surface, and the guiding directions of the guiding surfaces of adjacent two blocking members are arranged at an angle; an anesthesia gas monitoring device for testing the anesthesia gas concentration at the bottom of the receiving cavity; a counter installed below the receiving cavity and used to record the experimental insects to be tested passing through the recovery opening; and a recovery container provided below the recovery opening and used to recover the experimental insects.

[0007] In some embodiments, the blocking member includes: a fixing ring fixed to the side wall of the accommodating cavity; an arc-shaped plate connected to the fixing ring and defining a blocking cavity with the fixing ring. The bottom wall of the blocking cavity constitutes the guiding surface, and the blocking cavity has an opening facing the side wall of the accommodating cavity.

[0008] In some embodiments, the accommodating cavity includes a gradually expanding section, a first straight section, and a gradually shrinking section connected in sequence. The insertion opening is provided on the circumferential surface of the gradually expanding section, a plurality of the blocking members are provided in the first straight section, and the detection head of the anesthetic gas monitoring device is provided on the circumferential surface of the gradually shrinking section.

[0009] In some specific embodiments, the accommodating cavity further includes a second straight section connected to the small end of the gradually expanding section, and one end of the second straight section away from the gradually expanding section is connected to an external anesthetic gas source through an intake pipe.

[0010] In some embodiments, the accommodating cavity includes a third straight section, and the lower end of the third straight section forms the recovery opening. The counter includes: a first display having a timing display area and a counting display area; a light-emitting transistor provided on the inner side wall of the third straight section; and an infrared light source provided on the inner side wall of the third straight section and corresponding to the light-emitting transistor.

[0011] In some embodiments, the counter is further provided with a timing start button, a timing pause button, and a zeroing button.

[0012] In some embodiments, a control valve is provided above the recovery opening.

[0013] In some embodiments, the accommodating cavity is further provided with an air extraction joint above the recovery opening; the insect anesthesia automatic monitoring system further includes an air extraction device connected to the air extraction joint, and the air extraction device is used to extract the anesthetic gas in the accommodating cavity.

[0014] In some specific embodiments, the air extraction device includes a housing and a vacuum air extraction pump provided in the housing, and activated carbon is provided between the housing and the vacuum air extraction pump.

[0015] In some embodiments, the anesthetic gas monitoring device includes a second display and a detection head. The detection head is provided on the inner side wall of the accommodating cavity and above the recovery opening, and the second display is used to display the concentration of the anesthetic gas detected by the detection head.

[0016] Advantages of the automatic insect anesthesia monitoring system of the present invention: During the actual working process, an external anesthesia gas source sends anesthesia gas into the accommodation cavity from the air inlet, and then blows experimental insects into the accommodation cavity from the loading opening. Since there are multiple blocking members vertically and spaced apart in the accommodation cavity, the experimental insects fall vertically onto the multiple blocking members after being anesthetized, and continue to fall downward under the guiding action of the guiding surface, and finally enter the recovery container from the recovery opening. The experimental insects that are not fully anesthetized will adjust their postures during the falling process and will not immediately fall into the recovery container, which can better distinguish between sleeping and anesthetized insects, is easy to observe, and meets the experimental requirements such as screening for anesthesia sensitivity of different genotypes. At the same time, the added anesthesia gas monitoring device is used to test the anesthesia gas concentration at the bottom of the accommodation cavity, and the added counter is installed below the accommodation cavity and is used to record the experimental insects to be tested passing through the recovery opening, facilitating insect counting and real-time monitoring of the anesthesia gas concentration, and facilitating the monitoring and data recording of anesthesia experiments.

[0017] Additional aspects and advantages of the present invention will be given in part in the following description, will become apparent in part from the following description, or will be understood through the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 is a schematic structural diagram of the automatic insect anesthesia monitoring system according to an embodiment of the present invention;

[0019] Figure 2 is a schematic structural diagram of the test container according to an embodiment of the present invention;

[0020] Figure 3 is a schematic structural diagram of the counter according to an embodiment of the present invention;

[0021] Figure 4 is a schematic structural diagram of the air extraction device according to an embodiment of the present invention.

[0022] Reference numerals:

[0023] 100, test container; 110, accommodation cavity; 111, gradually expanding section; 112, first straight section; 113, gradually contracting section; 114, second straight section; 115, third straight section; 120, air inlet; 130, loading opening; 140, recovery opening; 150, blocking member; 151, fixing ring; 152, arc plate; 160, control valve; 170, air extraction joint;

[0024] 200, anesthesia gas monitoring device; 210, second display; 220, detection head;

[0025] 300. Counter; 310. First display; 311. Timing display area; 312. Counting display area; 313. Timing start button; 314. Timing pause button; 315. Zeroing button; 320. Light-emitting transistor; 330. Infrared light source;

[0026] 400. Recycling container;

[0027] 500. Air extraction device; 510. Housing; 520. Vacuum air pump. Detailed implementation manners

[0028] To make the technical problems solved by the present invention, the technical solutions adopted and the achieved technical effects clearer, the technical solutions of the present invention will be further described below with reference to the accompanying drawings and through specific implementation manners.

[0029] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0030] In addition, features defined with "first" and "second" may explicitly or implicitly include one or more of such features, used to distinguish and describe features, without order or importance. In the description of the present invention, unless otherwise specified, the meaning of "a plurality" is two or more.

[0031] In the description of the present invention, it should be noted that, unless otherwise clearly defined and limited, the terms "installed", "connected", "connected" should be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be directly connected, or indirectly connected through an intermediate medium, and it may be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0032] Next, refer to Figures 1-4 Describe the specific structure of the automatic insect anesthesia monitoring system according to an embodiment of the present invention.

[0033] The present invention discloses an automatic insect anesthesia monitoring system, as Figure 1 and Figure 2As shown, the automatic insect anesthesia monitoring system includes a test container 100, an anesthesia gas monitoring device 200, a counter 300, and a recovery container 400. The test container 100 has a receiving cavity 110. The top of the receiving cavity 110 has an air inlet 120 and a placement opening 130, and the bottom is provided with a recovery opening 140. The air inlet 120 is used to connect to an external anesthesia gas source, the placement opening 130 is used to place experimental insects, and the receiving cavity 110 is further provided with a plurality of blocking members 150 distributed at vertical intervals. Each blocking member 150 has an inclined guiding surface, and the guiding directions of the guiding surfaces of two adjacent blocking members 150 are arranged at an angle. The anesthesia gas monitoring device 200 is used to test the anesthesia gas concentration at the bottom of the receiving cavity 110. The counter 300 is installed below the receiving cavity 110 and is used to record the experimental insects to be tested passing through the recovery opening 140. The recovery container 400 is arranged below the recovery opening 140 and is used to recover the experimental insects.

[0034] It can be understood that during the actual working process, the external anesthesia gas source sends the anesthesia gas into the receiving cavity 110 from the air inlet 120, and then blows the experimental insects into the receiving cavity 110 from the placement opening 130. Since a plurality of blocking members 150 are arranged at vertical intervals in the receiving cavity 110, the experimental insects fall vertically onto the plurality of blocking members 150 after being anesthetized, and continue to fall downward under the guiding action of the guiding surface, and finally enter the recovery container 400 from the recovery opening 140. The experimental insects that are not fully anesthetized will adjust their postures during the falling process and will not immediately fall into the recovery container 400, which can better distinguish between sleeping and anesthetized insects, is easy to observe, and meets the experimental requirements such as screening for anesthesia sensitivity of different genotypes. At the same time, the added anesthesia gas monitoring device 200 is used to test the anesthesia gas concentration at the bottom of the receiving cavity 110, and the added counter 300 is installed below the receiving cavity 110 and is used to record the experimental insects to be tested passing through the recovery opening 140, which facilitates insect counting and real-time monitoring of the anesthesia gas concentration, and is convenient for monitoring the anesthesia experiment and data recording.

[0035] In some embodiments, as Figure 2 shown, the blocking member 150 includes a fixing ring 151 and an arc plate 152. The fixing ring 151 is fixed to the side wall of the receiving cavity 110. The arc plate 152 is connected to the fixing ring 151 and defines a blocking cavity with the fixing ring 151. The bottom wall of the blocking cavity constitutes the guiding surface, and the blocking cavity has an opening facing the side wall of the receiving cavity 110. It can be understood that the fixing ring 151 can ensure that the entire blocking member 150 is stably fixed in the receiving cavity 110, and the arc plate 152 ensures that the experimental insects can slide into the recovery container 400 under the guiding action of the guiding surface.

[0036] In some embodiments, as Figure 2As shown, the accommodation cavity 110 includes a gradually expanding section 111, a first straight section 112, and a gradually contracting section 113 that are connected in sequence. The inlet 130 is provided on the circumferential surface of the gradually expanding section 111. A plurality of blocking members 150 are provided in the first straight section 112. The detection head 220 of the anesthetic gas monitoring device 200 is provided on the circumferential surface of the gradually contracting section 113. It can be understood that the plurality of blocking members 150 are provided in the first straight section 112 to ensure that each experimental insect can first pass through the blocking members 150 and then fall into the recovery container 400, extending the time of the experimental insect in the accommodation cavity 110, ensuring that the experimental insect is completely anesthetized, and facilitating the distinction between sleeping and anesthetized experimental insects. The inlet 130 is provided on the circumferential surface of the gradually expanding section 111, which can provide enough space to blow in the experimental insects and increase the number of experimental insects that can be blown in during one experiment.

[0037] In some specific embodiments, as Figure 2 shown, the accommodation cavity 110 further includes a second straight section 114. The second straight section 114 is connected to the small end of the gradually expanding section 111, and the end of the second straight section 114 away from the gradually expanding section 111 is connected to an external anesthetic gas source through an intake pipe. It can be understood that by providing the second straight section 114 and connecting it to the external anesthetic gas source using an intake pipe, on the one hand, it is convenient to connect the test container 100 to the external anesthetic gas source and facilitate experimental operations. On the other hand, the second straight section 114 can be inserted into the intake pipe, improving the connection seal and avoiding the leakage of anesthetic gas.

[0038] In some embodiments, as Figure 2 shown, the accommodation cavity 110 includes a third straight section 115, and the lower end of the third straight section 115 forms a recovery port 140. As Figure 3 shown, the counter 300 includes a first display 310, a light-emitting transistor 320, and an infrared light source 330. The first display 310 has a timing display area 311 and a counting display area 312. The light-emitting transistor 320 is provided on the inner side wall of the third straight section 115, and the infrared light source 330 is provided on the inner side wall of the third straight section 115 and is arranged corresponding to the light-emitting transistor 320. It can be understood that during the actual working process, when the experimental insect falls, the light emitted by the infrared light source 330 cannot irradiate the light-emitting transistor 320. In this way, the signal received by the control system of the counter 300 from the light-emitting transistor 320 is in the form of pulses, and through an internal conversion program, the pulse signal can be converted into a digital display in the counting display area 312, thus facilitating the experimenter to record the number of experimental insects. The timing display area 311 can display the experimental duration, further facilitating experimental records.

[0039] In some embodiments, as Figure 3As shown, the counter 300 is also provided with a timing start button 313, a timing pause button 314, and a zero reset button 315. It can be understood that during the experiment, the control of the timing start button 313, the timing pause button 314, and the zero reset button 315 can be used to record experimental data within a specified duration, facilitating the user to conduct the experiment.

[0040] It should be supplemented here that the control logic of the control system built into the counter 300, the timing display area 311, the counting display area 312, the timing start button 313, the timing pause button 314, and the zero reset button 315 are all prior arts and will not be described here.

[0041] In some embodiments, as Figure 2 shown, a control valve 160 is provided above the recovery port 140. It can be understood that the control valve 160 can be opened or closed according to the needs of the experiment, thereby realizing the counting function according to actual needs.

[0042] In some embodiments, as Figures 1-2 shown, the accommodation chamber 110 is further provided with an air extraction joint 170, and the air extraction joint 170 is located above the recovery port 140. The insect anesthesia automatic monitoring system further includes an air extraction device 500, and the air extraction device 500 is connected to the air extraction joint 170. The air extraction device 500 is used to extract the anesthetic gas in the accommodation chamber 110. It can be understood that after the experiment, it is necessary to recover the anesthetic gas in the accommodation chamber 110, and during the experiment, it is also necessary to ensure that the anesthetic gas in the accommodation chamber 110 is in a relatively balanced state. The added air extraction device 500 can facilitate the recovery of the anesthetic gas and keep the anesthetic gas in the accommodation chamber 110 at a specified experimental concentration.

[0043] In some specific embodiments, as Figure 4 shown, the air extraction device 500 includes a housing 510 and a vacuum air extraction pump 520 provided in the housing 510. An activated carbon is provided between the housing 510 and the vacuum air extraction pump 520. Thus, the vacuum air extraction pump 520 can achieve air extraction, and the activated carbon can adsorb the anesthetic gas, avoiding the escape of the anesthetic gas into the experimental environment and ensuring the safety of the experiment.

[0044] In some embodiments, as Figure 1 shown, the anesthetic gas monitoring device 200 includes a second display 210 and a detection head 220. The detection head 220 is provided on the inner side wall of the accommodation chamber 110 and above the recovery port 140. The second display 210 is used to display the concentration of the anesthetic gas detected by the detection head 220. It can be understood that the cooperation of the second display 210 and the detection head 220 can facilitate the experimenter to monitor and observe the concentration of the anesthetic gas in real time, facilitating the progress of the experiment.

[0045] Embodiment:

[0046] As Figures 1-4 shown, the automatic insect anesthesia monitoring system includes a test container 100, an anesthesia gas monitoring device 200, a counter 300, and a recovery container 400. The test container 100 has a receiving cavity 110. The receiving cavity 110 includes a second straight section 114, a gradually expanding section 111, a first straight section 112, a gradually contracting section 113, and a third straight section 115 that are connected in sequence. Eight spaced-apart blocking members 150 are provided in the first straight section 112. The blocking member 150 includes a fixing ring 151 and an arc-shaped plate 152. The fixing ring 151 is fixed to the side wall of the first straight section 112. The arc-shaped plate 152 is connected to the fixing ring 151 and defines a blocking cavity with the fixing ring 151. The bottom wall of the blocking cavity constitutes a guiding surface, and the blocking cavity has an opening facing the side wall of the receiving cavity 110. The guiding surfaces of two spaced-apart blocking members 150 are arranged in parallel. A placing opening 130 is provided on the circumferential surface of the gradually expanding section 111. The placing opening 130 is used to place experimental insects. An air extraction joint 170 is provided on the gradually contracting section 113. One end of the second straight section 114 away from the gradually expanding section 111 is connected to an external anesthesia gas source through an intake pipe. The lower end of the third straight section 115 forms a recovery opening 140. The counter 300 includes a first display 310, a light-emitting transistor 320, and an infrared light source 330. The first display 310 has a timing display area 311, a counting display area 312, a timing start button 313, a timing pause button 314, and a zeroing button 315. The light-emitting transistor 320 is provided on the inner side wall of the third straight section 115. The infrared light source 330 is provided on the inner side wall of the third straight section 115 and is arranged corresponding to the light-emitting transistor 320. A control valve 160 is provided above the recovery opening 140. The air extraction device 500 includes a housing 510 and a vacuum air extraction pump 520 provided in the housing 510. An activated carbon is provided between the housing 510 and the vacuum air extraction pump 520. The vacuum air extraction pump 520 is connected to the air extraction joint 170 through an air extraction pipe. The anesthesia gas monitoring device 200 includes a second display 210 and a detection head 220. The detection head 220 is provided on the inner side wall of the gradually contracting section 113. The second display 210 is used to display the concentration of the anesthesia gas detected by the detection head 220. The recovery container 400 is provided below the recovery opening 140 and is used to recover experimental insects.

[0047] The usage method of the automatic insect anesthesia detection system in this embodiment is as follows:

[0048] First: When the experimental purpose is to test the EC50 (concentration for 50% of maximal effect) of the Drosophila anesthetic gas, turn off the vacuum pump 520, open the control valve 160, remove the cap blocking the second straight section 114, and introduce the anesthetic gas into the accommodation chamber 110 through the inlet pipe. Observe the anesthetic gas concentration in the tapered section 113 through the second display 210. Blow each group of Drosophila into the inlet 130, turn on the power switch of the counter 300, press the timing start button 313. The dropped Drosophila interrupts the light beam emitted by the infrared light source 330, and the pulse signal is transmitted into the counter 300 to enable the counter 300 to automatically record the number of anesthetized Drosophila. Press the timing pause button 314 at the specified time, record the number of Drosophila, then press the zero button 315, close the control valve 160, calculate the ratio of the number of anesthetized Drosophila in each group to the total number of Drosophila, plot the dose-response curve, and calculate the EC50.

[0049] Second: When the experimental purpose is to screen the sensitivities of Drosophila with different genotypes, open the control valve 160, turn on the vacuum pump 520, remove the cap blocking the second straight section 114, and introduce the anesthetic gas into the accommodation chamber 110 through the inlet pipe. Observe the anesthetic gas concentration in the tapered section 113 through the second display 210. After the gas concentration in the accommodation chamber 110 is balanced, blow a large number of Drosophila of the tested genotype into the inlet 130, turn on the power switch of the counter 300, press the timing start button 313. The dropped Drosophila interrupts the light beam emitted by the infrared light source 330, and the pulse signal is transmitted into the counter 300 to enable the counter 300 to automatically record the number of anesthetized Drosophila. Press the timing pause button 314 at the specified time (30 minutes), record the number of Drosophila, then press the zero button 315. Replace the Drosophila with different genotypes, press the timing start button 313 again to repeat the experiment, calculate the response indices of different genotypes, and compare the sensitivities.

[0050] In the description of this specification, the descriptions referring to terms such as "some embodiments", "other embodiments", etc. mean that the specific features, structures, materials, or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials, or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0051] The above content is only the preferred embodiment of the present invention. For those of ordinary skill in the art, according to the idea of the present invention, there will be changes in the specific implementation manners and application scopes. The content of this specification should not be construed as a limitation to the present invention.

Claims

1. An automatic insect anesthesia monitoring system, characterized in that, it includes: A test container (100), the test container (100) has a receiving cavity (110), the top of the receiving cavity (110) has an air inlet (120) and a placing port (130), and the bottom is provided with a recovery port (140). The air inlet (120) is used to connect to an external anesthesia gas source, the placing port (130) is used to place experimental insects, and the receiving cavity (110) is also provided with a plurality of blocking members (150) distributed at intervals along the vertical direction. Each of the blocking members (150) has an inclined guiding surface, and the guiding directions of the guiding surfaces of two adjacent blocking members (150) are arranged at an angle; An anesthesia gas monitoring device (200), the anesthesia gas monitoring device (200) is used to test the anesthesia gas concentration at the bottom of the receiving cavity (110); A counter (300), the counter (300) is installed below the receiving cavity (110) and is used to record the experimental insects to be tested passing through the recovery port (140); A recovery container (400), the recovery container (400) is arranged below the recovery port (140) and is used to recover the experimental insects.

2. The automatic insect anesthesia monitoring system according to claim 1, characterized in that, The blocking member (150) includes: A fixing ring (151), the fixing ring (151) is fixed to the side wall of the receiving cavity (110); An arc-shaped plate (152), the arc-shaped plate (152) is connected to the fixing ring (151), and defines a blocking cavity with the fixing ring (151). The bottom wall of the blocking cavity constitutes the guiding surface, and the blocking cavity has an opening facing the side wall of the receiving cavity (110).

3. The automatic insect anesthesia monitoring system according to claim 1, characterized in that, The receiving cavity (110) includes a gradually expanding section (111), a first straight section (112) and a gradually shrinking section (113) connected in sequence. The placing port (130) is arranged on the circumferential surface of the gradually expanding section (111), and a plurality of the blocking members (150) are arranged in the first straight section (112). The detection head (220) of the anesthesia gas monitoring device (200) is arranged on the circumferential surface of the gradually shrinking section (113).

4. The automatic insect anesthesia monitoring system according to claim 3, characterized in that, The receiving cavity (110) further includes a second straight section (114). The second straight section (114) is connected to the small end of the gradually expanding section (111), and one end of the second straight section (114) far from the gradually expanding section (111) is connected to the external anesthesia gas source through an air inlet pipe.

5. The automatic insect anesthesia monitoring system according to claim 1, characterized in that, The receiving cavity (110) includes a third straight section (115). The lower end of the third straight section (115) forms the recovery port (140). The counter (300) includes: A first display (310), the first display (310) having a timing display area (311) and a counting display area (312); A light-emitting transistor (320), the light-emitting transistor (320) being provided on the inner sidewall of the third straight section (115); An infrared light source (330), the infrared light source (330) being provided on the inner sidewall of the third straight section (115) and arranged corresponding to the light-emitting transistor (320).

6. The automatic insect anesthesia monitoring system according to claim 1, characterized in that a timing start button (313), a timing pause button (314), and a zeroing button (315) are further provided on the counter (300).

7. The automatic insect anesthesia monitoring system according to claim 1, characterized in that a control valve (160) is provided above the recovery port (140).

8. The automatic insect anesthesia monitoring system according to claim 1, characterized in that an air extraction joint (170) is further provided in the accommodation cavity (110), and the air extraction joint (170) is located above the recovery port (140); the automatic insect anesthesia monitoring system further includes an air extraction device (500), the air extraction device (500) is connected to the air extraction joint (170), and the air extraction device (500) is used to extract the anesthesia gas in the accommodation cavity (110).

9. The automatic insect anesthesia monitoring system according to claim 8, characterized in that the air extraction device (500) includes a housing (510) and a vacuum air extraction pump (520) provided in the housing (510), and activated carbon is provided between the housing (510) and the vacuum air extraction pump (520).

10. The automatic insect anesthesia monitoring system according to claim 1, characterized in that the anesthesia gas monitoring device (200) includes a second display (210) and a detection head (220), the detection head (220) is provided on the inner sidewall of the accommodation cavity (110) and above the recovery port (140), and the second display (210) is used to display the concentration of the anesthesia gas detected by the detection head (220).

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