Application of composition based on methyl carboline acid in plant disease resistance

Abstract

The invention discloses an application of a composition based on methyl carboline acid in plant disease resistance, and the effective components comprise 1-methyl-1, 2, 3, 4-tetrahydro-beta-carboline-3 carboxylic acid and a bactericide, the bactericide is selected from one or a combination of two or more of 20% of moroxydine hydrochloride, 30% of Dufulin, 50% of chlorobromoisocyanuric acid, 20% of fosthiazate and 41.7% of fluopyram. According to the present invention, after the antiviral compositions are compounded according to the ratio, the disease resistance and the control efficacy can be significantly improved, and the amount of the pesticide used in the farmland can be substantially reduced, such that the environmental pollution and the pesticide residue can be reduced, the production cost can be reduced, and the application range of the pesticide can be widened; and compared with the defect of fewer application objects of a single agent, the compound composition can be well applied to crops such as vegetables, melons and fruits, tobaccos and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of pesticides, and particularly relates to the application of a composition based on methylcarbolinic acid in plant disease resistance. Background Art

[0002] In agricultural production, effective control of diseases is crucial for ensuring the healthy growth of crops. However, even highly efficient fungicides, when used alone for a long time, can cause crops to develop drug resistance, resulting in an increase in the dosage of the agent, a decrease in the drug efficacy, and a shortening of the drug effect period. Therefore, scientifically and reasonably applying fungicides, reducing the dosage, and improving the drug efficacy have become urgent problems to be solved in the field of plant control.

[0003] Chinese Patent Document CN117204433A (Application No.: 201510059660.1) discloses the application and preparation method of compound 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid. This patent document discloses the preparation and application of a compound methylcarbolinic acid obtained by separating and purifying from Pleurotus passeckerianus YXI. This patent document discloses that this compound has the effects of drought resistance, antiviral activity, and promoting plant growth and increasing yield when compounded with fertilizers, but does not conduct in-depth research on the antiviral and anti-nematode effects of compounding this compound with other agents. In 2023, this compound was approved and named by the National Technical Committee for Pesticide Standardization, and the Chinese common name was determined as "methylcarbolinic acid". Summary of the Invention

[0004] To solve the problems existing in the current use of pesticides, such as large pesticide usage and poor effects, the present invention provides the application of a composition based on methylcarbolinic acid in plant disease resistance.

[0005] In order to achieve the above technical objectives, the technical solutions implemented by the present invention are as follows:

[0006] In a first aspect, the present invention provides a composition based on methylcarbolinic acid, the active ingredients of which include 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid and a fungicide; the fungicide is selected from moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid, and one or a combination of two or more components selected from fosthiazate and fluopyram.

[0007] Preferably, the composition based on methylcarbolinic acid contains moroxydine hydrochloride, dufulin, or chlorobromoisocyanuric acid; preferably, the moroxydine hydrochloride is 20% moroxydine hydrochloride, the dufulin is 30% dufulin, and the chlorobromoisocyanuric acid is 50% chlorobromoisocyanuric acid.

[0008] Furthermore, in the composition, the mass ratio of methylcarbolinic acid, 20% moroxydine hydrochloride, 30% dufulin, or 50% chlorobromoisocyanuric acid is 1:50 to 1:1, preferably 1:25 to 1:1, more preferably 1:10 to 1:1.

[0009] In a second aspect, the present invention also provides the use of the composition comprising moroxydine hydrochloride, dufulin or chlorobromoisocyanuric acid in reducing the incidence of bacterial wilt virus disease in crops such as tomatoes.

[0010] In a third aspect, the present invention also provides the use of the composition comprising moroxydine hydrochloride, dufulin or chlorobromoisocyanuric acid in reducing the incidence of peanut mosaic virus disease in crops.

[0011] In a fourth aspect, the present invention also provides the use of the composition comprising moroxydine hydrochloride, dufulin or chlorobromoisocyanuric acid in reducing the incidence of turnip mosaic virus disease in crops.

[0012] Preferably, the methyl carbolic acid-based composition comprises 20% fosthiazate or 41.7% fluxapyroxad.

[0013] Furthermore, in the composition, the mass ratio of methyl carbolic acid, 20% fosthiazate or 41.7% fluxapyroxad is 1:50 to 1:1, preferably 1:25 to 1:1, more preferably 1:10 to 1:1.

[0014] In a fifth aspect, the present invention also provides the use of the composition comprising 20% fosthiazate and 41.7% fluxapyroxad in reducing nematode activity and thus controlling plant diseases and pests. Further, the plant diseases and pests are nematode diseases.

[0015] After adopting this technical solution, 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid (methyl carboline acid) has a good antiviral effect on plants and achieves the disease resistance effect. It has high activity, can be mixed, has obvious synergistic effects, and has great development space. Moroxydine hydrochloride mainly prevents the replication process of the virus by inhibiting or destroying the formation of nucleic acid and lipoprotein, thus playing a role in preventing and controlling the virus. Dofequidar activates the tobacco salicylic acid signal transduction pathway, increases the content of the signal molecule salicylic acid, and thus promotes the expression of downstream pathogenesis-related proteins; it obtains antiviral ability by inducing the activities of crop PAL, POD, and SOD defense enzymes. Chlorobromoisocyanuric acid sprayed on the surface of crops can slowly release hypobromous acid (HOBr) and hypochlorous acid (HOCl). Hypobromous acid is 4 times more active than hypochlorous acid and has a strong ability to kill bacteria and fungi. After the parent body releases hypobromous acid through systemic conduction, it forms triazinedione (DH) and sym-triazine (AdHL), which have a strong virucidal effect. It not only has a strong ability to prevent and kill bacteria, fungi, and viruses, but also has the effect of promoting the vegetative growth of crops. Methyl carboline acid, moroxydine hydrochloride, and dofequidar all belong to the category of inducible resistance agents. Therefore, the compounded agent can not only enhance the drug effect, but also reduce the dosage of single-agent components, save the drug cost. The co-toxicity coefficient of the compounded agent is calculated through indoor toxicity determination tests, and a value higher than 120 indicates a synergistic effect.

[0016] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0017] 1. In the present invention, experiments show that after compounding according to the described ratio, each antiviral composition can significantly improve the resistance to diseases and the control efficacy, and at the same time can greatly reduce the amount of pesticides used in farmland. Therefore, it can also reduce environmental pollution and pesticide residues, and at the same time can reduce production costs and broaden the scope of application of the drug. Compared with the defect that the application object of a single agent is less, this compound combination can be well applied to crops such as vegetables, fruits, and tobacco;

[0018] 2. It is found that the combined application of methyl carboline acid with two nematicides, fosthiazate and fluxapyroxad, has an effect on improving the nematicidal activity of plants;

[0019] 3. The absolute dosage is small. Experiments show that 1 mg of methyl carboline acid compounded with moroxydine hydrochloride, dofequidar, chlorobromoisocyanuric acid, fosthiazate, and fluxapyroxad can be sprayed on 1 mu of crops and has an obvious antiviral and nematicidal effect. Detailed implementation manners

[0020] It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the present invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present invention belongs.

[0021] The technical solution of the present invention will be further described below in conjunction with specific embodiments. The described embodiments are only a part of this application, not all embodiments.

[0022] Example 1 Control effect of the composition of methylcarbolinic acid and various antiviral agents on plants

[0023] 1. Determination of the control effect of the compounding of methylcarbolinic acid with moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid on tomato bacterial wilt virus

[0024] (1) Experimental design:

[0025] For tobacco seedling raising, after two weeks, select seedlings of the same size and transplant them into small powder pots. Put every 5 pots into a tray. After 5 days of slow seedling stage, spray the treatment solution to make the treatment solution evenly adhere to the surface of tobacco leaves. After 24 hours, evenly sprinkle quartz powder on the tobacco leaves, add 10 μL of the disease solution, and gently rub. After 7 days, observe the disease occurrence of tobacco leaves under fluorescence. Measure the control effects of different concentrations of each test agent, and calculate the control effect after taking the average value.

[0026] The test agents include methylcarbolinic acid prepared in the laboratory, 20% moroxydine hydrochloride, 30% dufulin, and 50% chlorobromoisocyanuric acid.

[0027] (2) Calculation method

[0028] Calculate the virulence index and co-toxicity coefficient (CTC) of the agent according to the Sun Yunpei method.

[0029] Actual measured virulence index (ATI) = (standard agent EC50 / test agent EC50) × 100;

[0030] Theoretical virulence index (TTI) = virulence index of agent A × percentage content of A in the mixture + virulence index of agent B × percentage content of B in the mixture;

[0031] Co-toxicity coefficient (CTC) = [actual measured virulence index of the mixture (ATI) / theoretical virulence index of the mixture (TTI)] × 100;

[0032] When CTC ≤ 80, the composition shows an antagonistic effect; when 80 < CTC < 120, the composition shows an additive effect; when CTC ≥ 120, the composition shows a synergistic effect. See Table 1 for the results of the efficacy determination (Note: In Table 1, except that the active ingredients are changed according to the ratio of the agents in Table 1, other conditions are exactly the same).

[0033] Table 1 Indoor virulence determination results of the series of ratios of methylcarbolinic acid, moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid on tomato bacterial wilt virus

[0034]

[0035] (3) Test results

[0036] The test results show that when the ratio of methylcarbolinic acid to moroxydine hydrochloride is between 1:25 and 1:1, the co-toxicity coefficient (CTC) is higher than 120, showing a synergistic effect. When the ratio of methylcarbolinic acid to moroxydine hydrochloride is between 1:10 and 1:1, the EC50 of the composition is less than that of methylcarbolinic acid and moroxydine hydrochloride alone, proving that the use of this composition can reduce the dosage of methylcarbolinic acid and moroxydine hydrochloride; when methylcarbolinic acid and dufulin are compounded at a mass ratio of 1:50 to 1:1 for controlling tomato bacterial wilt virus, the co-toxicity coefficient (CTC) is above 110. When the ratio is between 1:25 and 1:1, the co-toxicity coefficient (CTC) is higher than 120, showing a synergistic effect. When the ratio is between 1:10 - 1:1, the co-toxicity coefficient is higher than 150, and the synergistic effect is significant. When the ratio of methylcarbolinic acid to dufulin is between 1:10 and 1:1, the EC50 of the composition is less than that of methylcarbolinic acid and dufulin alone, proving that the use of this composition can reduce the dosage of methylcarbolinic acid and dufulin; when methylcarbolinic acid and chlorobromoisocyanuric acid are compounded at a mass ratio of 1:50 to 1:1 for controlling tomato bacterial wilt virus, the co-toxicity coefficient (CTC) is above 90, all showing a synergistic effect. When the ratio is between 1:25 and 1:1, the co-toxicity coefficient (CTC) is higher than 120, showing a synergistic effect. When the ratio is between 1:10 - 1:1, the co-toxicity coefficient is higher than 150, and the synergistic effect is significant.

[0037] 2. Efficacy determination of the compounding of methylcarbolinic acid with moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid against peanut yellow mosaic virus

[0038] (1) Test design:

[0039] The above method was used to determine the efficacy of the compounding of methylcarbolinic acid with moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid against turnip mosaic virus.

[0040] (2) Test results

[0041] Table 2 Indoor virulence determination results of the series ratios of methylcarbolinic acid and moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid against peanut yellow mosaic virus

[0042]

[0043]

[0044] (3) Test results

[0045] The test results show that when methylcarbolinic acid and moroxydine hydrochloride are compounded at a mass ratio of 1:50 to 1:1, the co-toxicity coefficient (CTC) for controlling peanut yellow mosaic virus is above 90. When the ratio is 1:50, it shows an additive effect. When the ratio is between 1:25 and 1:1, it has a synergistic effect. When the ratio is 1:1, the co-toxicity coefficient (CTC) is higher than 150, and the synergistic effect is significant. When methylcarbolinic acid and dufulin are compounded at a mass ratio of 1:50 to 1:1, the co-toxicity coefficient (CTC) for controlling peanut yellow mosaic virus is above 110. When the ratio is between 1:25 and 1:1, the co-toxicity coefficient (CTC) is higher than 120, showing a synergistic effect. When methylcarbolinic acid and chlorobromoisocyanuric acid are compounded at a mass ratio of 1:50 to 1:1, the co-toxicity coefficient (CTC) for controlling peanut yellow mosaic virus is above 90. When the ratio is between 1:25 and 1:1, the co-toxicity coefficient is above 120, showing a synergistic effect. When the ratio is between 1:10 and 1:1, the co-toxicity coefficient (CTC) is higher than 150, and the synergistic effect is significant.

[0046] When methylcarbolinic acid is compounded with moroxydine hydrochloride and chlorobromoisocyanuric acid at a ratio of 1:25 to 1:1, the EC50 of the composition is less than that of the single agent of the medicament, which proves that the use of this composition can reduce the usage amounts of methylcarbolinic acid, moroxydine hydrochloride, and chlorobromoisocyanuric acid. When methylcarbolinic acid and dufulin are compounded, the EC50 of all compositions is less than that of the single agents of methylcarbolinic acid and dufulin, which proves that the above ratios can effectively reduce the usage amounts of methylcarbolinic acid and dufulin.

[0047] 3. Determination of the control effect of the compound of methylcarbolinic acid with moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid against turnip mosaic virus

[0048] (1) Test design:

[0049] The above method was used to determine the control effect of the compound of methylcarbolinic acid with moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid against turnip mosaic virus.

[0050] (2) Test results

[0051] Table 3 Indoor toxicity determination results of the series ratios of methylcarbolinic acid and moroxydine hydrochloride, dufulin, and chlorobromoisocyanuric acid against turnip mosaic virus

[0052]

[0053]

[0054] (3) Determination results

[0055] The test results showed that when methylcarbolinic acid and moroxydine hydrochloride were compounded at a ratio of 1:50 to 1:1, the co-toxicity coefficient (CTC) for controlling turnip mosaic virus was above 100. When the ratio was between 1:25 and 1:1, there was a synergistic effect. When the ratio was between 1:10 and 1:1, the co-toxicity coefficient (CTC) was higher than 150, and the synergistic effect was significant. The EC50 of each composition of methylcarbolinic acid and moroxydine hydrochloride was less than that of the single agents of methylcarbolinic acid and moroxydine hydrochloride, indicating that the use of this composition could reduce the usage amounts of methylcarbolinic acid and moroxydine hydrochloride. When methylcarbolinic acid and dufulin were compounded at a mass ratio of 1:50 to 1:1, the co-toxicity coefficient (CTC) for controlling turnip mosaic virus was above 120, and there was a synergistic effect. When the ratio was between 1:25 and 1:1, the co-toxicity coefficient (CTC) was higher than 150, and the synergistic effect was significant. When the ratio of methylcarbolinic acid and dufulin was between 1:25 and 1:1, the EC50 of the composition was less than that of the single agents of methylcarbolinic acid and dufulin, and the usage amounts of methylcarbolinic acid and dufulin could be reduced. When methylcarbolinic acid and chlorobromoisocyanuric acid were compounded at a mass ratio of 1:50 to 1:1, the co-toxicity coefficient (CTC) for controlling turnip mosaic virus was above 100, and there was a synergistic effect. When the ratio was between 1:25 and 1:1, the co-toxicity coefficient (CTC) was higher than 120, and there was a synergistic effect. When the ratio was between 1:10 and 1:1, the co-toxicity coefficient (CTC) was higher than 150, and the synergistic effect was significant.

[0056] Example 2 Control effect of the composition of methylcarbolinic acid and nematicide on cucumber root-knot nematodes

[0057] 1. Screening of the compounding ratio of methylcarbolinic acid with fosthiazate and fluxapyroxad

[0058] (1) Experimental design:

[0059] Cucumbers were sown and raised normally. When they grew to the four-leaf and one-heart stage, cucumber seedlings with uniform growth were selected for transplanting. The experimental soil for transplanting was taken from a solar greenhouse in Dongdawu Village, Dawenkou Town, Daiyue District, Tai'an City, Shandong Province (117°8'21"E, 35°59'7"N) where root-knot nematode disease occurred all year round. The soil samples were taken from the field, sieved (2.5 mm), and the large particles were removed. After sieving, the soil was mixed evenly with the substrate (ratio about 2:1), potted, and cucumbers were transplanted. After transplanting, the seedlings were allowed to recover for 3 days before the experimental treatment. The experiment was set with 7 treatments of different ratios of methylcarbolinic acid, fosthiazate, and fluxapyroxad, and 200 mL of the treatment solution was irrigated into each pot. The experiment was treated once every 7 days (by irrigation), and each treatment had 5 replicates.

[0060] The tested agents included methylcarbolinic acid prepared in the laboratory, 20% fosthiazate, and 41.7% fluxapyroxad.

[0061] (2) Calculation method

[0062] The control effect is statistically analyzed based on the occurrence of root knots; the root knot classification standard is: level 0, no root knots; level 1, 010% root knots; level 2, 11 20% root knots; level 3, 21 30% root knots; level 4, 31 40% root knots; level 5, 41 50% root knots; level 6, 51 60% root knots; level 7, 61 70% root knots; level 8, 71 80% root knots; level 9, 81 90% root knots; level 10, 91 100% root knots;

[0063] Disease index = ∑ (number of diseased plants × corresponding root knot level) / (total number of plants × highest root knot level) × 100

[0064] Control effect (%) = [(disease index of control group - disease index of treatment group) / disease index of control group] × 100

[0065] The toxicity index and co-toxicity coefficient (CTC) of the agent were calculated using the same method as above.

[0066] Table 4 Results of indoor toxicity test of a series of combinations of methylcarboline acid, thiathiazolyl, and fluopyram against root-knot nematodes

[0067]

[0068]

[0069] (3) Measurement results

[0070] The test results show that when methylcarboline acid and thiazolylphos are mixed at a ratio of 1:50 to 1:1, the co-toxicity coefficient (CTC) for the control of southern root-knot nematodes is above 150, and the synergistic effect is significant; and when methylcarboline acid and morpholino hydrochloride are mixed at a weight ratio of 1:50 to 1:1, the half-effective dose is less than 40 mg / L, which can reduce the dosage of single-dose components and save drug costs. When methylcarboline acid and fluopyram are mixed at a ratio of 1:50 to 1:1, the co-toxicity coefficient (CTC) for the control of southern root-knot nematodes is above 120, and both have a synergistic effect. When the ratio is between 1:10 and 1:1, the co-toxicity coefficient (CTC) is higher than 150, and the synergistic effect is significant.

[0071] When the mixing ratio of methylcarbolinic acid and other agents is between 1:25 and 1:1, the median lethal concentration EC50 of the combined agent is lower than that of a single mixed agent, which proves that the application amount of the mixed agent is lower than that of a single agent and can reduce the usage of a single agent.

[0072] Combined with Examples 1 and 2, it can be seen that when the mixing ratio of methylcarbolinic acid to other agents is 1:1, the co-toxicity coefficient (CTC) exceeds 150, showing a very strong synergistic effect. As the addition amount of methylcarbolinic acid decreases, the co-toxicity coefficient (CTC) decreases accordingly. When the mixing ratio of methylcarbolinic acid to other agents is 1:25, the co-toxicity coefficient (CTC) is above 120, indicating that there is still a good synergistic effect at this time; when the mixing ratio of methylcarbolinic acid to other agents is 1:50, the median lethal concentration EC50 of the combined agent is less than that of the single agent of the mixed agent, proving that when in use, the dosage required for each agent in the mixed agent is lower than that when directly applying a single agent. Therefore, the dosage of a single agent can be reduced. The compounding of methylcarbolinic acid with a variety of chemical drugs expands the applicable range of methylcarbolinic acid and chemical drugs, and can control a variety of pests and diseases.

[0073] In summary, the tests show that: the bactericidal pesticide composition provided by the present invention can have obvious (1) synergistic effect; (2) reduce the dosage of drugs and lower the cost; (3) expand the applicable range of the agent.

[0074] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A composition based on methylcarboline acid, characterized in that The active ingredients include 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid and a fungicide; the fungicide is selected from one or a combination of two or more of guanidine hydrochloride, chlorpyrifos and chlorobromoisocyanuric acid, as well as thiazolylphos and fluopyram.

2. The composition based on methylcarbolinic acid according to claim 1, characterized in that The methylcarboline acid-based composition comprises guanidine hydrochloride, chlorfenapyr or chlorobromoisocyanuric acid; preferably, the guanidine hydrochloride is 20% guanidine hydrochloride, the chlorfenapyr is 30% chlorfenapyr, and the chlorobromoisocyanuric acid is 50% chlorobromoisocyanuric acid.

3. The composition based on methylcarbolinic acid according to claim 1, characterized in that The composition comprises methylcarboline acid, 20% morpholine hydrochloride, 30% chloranil or 50% chlorobromoisocyanuric acid in a mass ratio of 1:50 to 1:1, preferably 1:25 to 1:1, and more preferably 1:10 to 1:

1.

4. Use of the methylcarbolinic acid-based composition according to any one of claims 2 to 3 in reducing the incidence of tomato bacterial wilt virus disease in crops.

5. Use of the methylcarbolinic acid-based composition according to any one of claims 2 to 3 in reducing the incidence of peanut yellow mosaic virus disease in crops.

6. Use of the methylcarbolinic acid-based composition according to any one of claims 2 to 3 in reducing the incidence of turnip mosaic virus disease in crops.

7. The composition based on methylcarboline acid according to claim 1, characterized in that The methylcarbolic acid-based composition comprises 20% of thiazolyl or 41.7% of fluopyram.

8. The composition based on methylcarbolinic acid according to claim 1, characterized in that In the composition, the mass ratio of methylcarboline acid to 20% thiazolyl or 41.7% fluopyram is 1:50 to 1:1, preferably 1:25 to 1:1, and more preferably 1:10 to 1:

1.

9. Use of the methylcarbolinic acid-based composition according to any one of claims 7 to 8 in reducing nematode activity.

10. Use of the composition based on methylcarbolinic acid according to any one of claims 7 to 8 in preventing and controlling plant diseases and insect pests, wherein the plant diseases and insect pests are nematode diseases.

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