Rubber tree cut surface wound healing agent as well as preparation method and use method thereof

Abstract

The invention discloses a hevea brasiliensis cut surface wound healing agent as well as a preparation method and a use method thereof, and relates to the technical field of agriculture. Every 1000 mL of the hevea brasiliensis cut surface wound healing agent comprises the following components: 50 to 150 g of carboxymethyl chitosan, 50 to 200 g of clay, 0.1 to 1.2 mg of phytosulfokine, 0.1 to 1.0 g of indole-3-butyric acid potassium, 0.5 to 1.5 ml of eucalyptus citriodora oil, 0.2 to 1.0 g of polyethylene glycol, 1.0 to 10 g of MS dry powder, 2 to 10 g of sucrose and the balance of a solvent. The healing agent disclosed by the invention can effectively promote wound healing of cut surfaces of rubber trees, reduce generation of tree burls, stabilize the yield of the rubber trees, reduce dead skins and increase the utilization rate of regenerated skins.

Description

Technical Field

[0001] The present invention relates to the technical field of agriculture, and particularly relates to a wound healing agent for the tapping surface of rubber trees, a preparation method thereof, and a using method thereof. Background Art

[0002] Natural rubber, together with steel, petroleum, and coal, is known as the four major industrial raw materials, and is a basic industry and strategic material related to the national economy and people's livelihood. As one of the world's four major industrial raw materials, natural rubber is widely used in industries such as industry, agriculture, national defense, transportation, machinery manufacturing, medicine and health, and daily life due to its strong elasticity, good insulation, plasticity, wear resistance, tensile resistance, water and gas barrier properties, etc., and has become one of the indispensable material materials in national defense and industry. There are more than 2,000 rubber-producing plants in the world, but currently, the Hevea brasiliensis that is widely planted and has become the only commercial source of natural rubber.

[0003] The main purpose of commercially cultivating rubber trees is to obtain natural rubber. Tapping is the direct means of obtaining economic benefits from natural rubber and is also the only means at present. Tapping is a highly technical and delicate manual operation, with requirements for the tapping depth (conventional tapping 0.12 - 0.18 cm, stimulated tapping ≥ 0.18 cm), thickness (generally requiring 0.14 - 0.18 cm of bark consumption per cut for the sunny knife and 0.16 - 0.20 cm for the shady knife), and the slope of the tapping line (generally requiring 25 - 30° for sunny line tapping and 40 - 45° for shady line tapping). In particular, the requirement for the tapping depth is the strictest. If the tapping is too shallow, no yield can be obtained; if it is too deep, it is easy to damage the tree. It is required not to cut the phloem with conductive function (referred to as "water sac skin" by rubber tappers), and basically no major injuries, few minor injuries, and the smaller the wound, the better (special injury: wound area 0.4 cm * 1.0 cm, minor injury: wound area 0.25 cm * 0.25 cm, major injury: between special injury and minor injury). After the rubber tree starts tapping, it generally needs to be tapped continuously for decades, and the regenerated bark also needs to be tapped. If the rubber tree is cut, the wound will form a tumor, which will affect the growth of latex ducts, reduce the yield, accelerate the formation of dead bark, and in severe cases, the rubber tree may lose its value for tapping.

[0004] Tapping rubber is a very arduous task, usually carried out from early morning to dawn. The rubber plantations are located in mountainous areas with dense forests, where workers are bitten by mosquitoes and even at risk of being attacked by venomous snakes. The working environment is harsh. Workers need to tap rubber for four or five hours every day, and also collect the latex, transport it and manage the rubber plantation on a daily basis, involving heavy physical labor. Due to the special nature of rubber tapping production work, it has little attraction for young people, making it difficult to replenish the fresh blood in the tapping workforce. The shortage of rubber tappers is becoming increasingly serious, the workforce is aging, and there is a severe lack of skilled rubber tappers. Against this backdrop, it is inevitable that rubber trees are cut and damaged, and the situation is worsening. Especially in private rubber plantations, the tapping skills of rubber farmers are generally poor, resulting in serious damage to the trees, severe dead bark, large-scale reduction in latex production in the plantations, serious regeneration of bark nodules, and the inability to tap rubber after about 10 years of tapping, seriously shortening the economic lifespan of rubber trees. To mitigate the impact of cuts on rubber trees, it is necessary to develop a wound healing agent for the cut surfaces of rubber trees to accelerate wound healing, reduce the formation of nodules, stabilize latex production of rubber trees, reduce dead bark, and at the same time increase the utilization rate of regenerated bark. Summary of the Invention

[0005] One of the objectives of the present invention is to provide a wound healing agent for the cut surfaces of rubber trees, which can effectively promote the healing of the cut surfaces of rubber trees.

[0006] Another objective of the present invention is to provide a preparation method for the wound healing agent for the cut surfaces of rubber trees.

[0007] A further objective of the present invention is to provide a usage method for the wound healing agent for the cut surfaces of rubber trees.

[0008] To achieve the above objectives of the present invention, the following technical solutions are specifically adopted:

[0009] In the first aspect, the present invention provides a wound healing agent for the cut surfaces of rubber trees. Every 1000 mL of the wound healing agent for the cut surfaces of rubber trees comprises the following components: 50 - 150 g of carboxymethyl chitosan, 50 - 200 g of clay, 0.1 - 1.2 mg of phytosulfokine, 0.1 - 1.0 g of potassium indolebutyrate, 0.5 - 1.5 mL of lemon eucalyptus oil, 0.2 - 1.0 g of polyethylene glycol, 1.0 - 10 g of MS powder, 2 - 10 g of sucrose, and the rest is solvent.

[0010] Preferably, every 1000 mL of the wound healing agent for the cut surfaces of rubber trees comprises the following components: 80 - 120 g of carboxymethyl chitosan, 100 - 150 g of clay, 0.5 - 1.0 mg of phytosulfokine, 0.3 - 0.8 g of potassium indolebutyrate, 0.6 - 1.0 mL of lemon eucalyptus oil, 0.3 - 0.8 g of polyethylene glycol, 2.0 - 6 g of MS powder, 4 - 8 g of sucrose, and the rest is solvent.

[0011] More preferably, every 1000 mL of the wound healer for rubber tree tapping surfaces comprises the following components: 100 g of carboxymethyl chitosan, 150 g of clay, 0.8 mg of phytosulfokine, 0.5 g of potassium indolebutyrate, 0.75 mL of lemon eucalyptus oil, 0.67 g of polyethylene glycol, 4.74 g of MS powder, 6 g of sucrose, and the balance is solvent.

[0012] Preferably, the dosage form of the healer is an ointment, and the solvent is water.

[0013] Preferably, the carboxymethyl chitosan is one or more of N-carboxymethyl chitosan, O-carboxymethyl chitosan, and N,O-carboxymethyl chitosan. In order to obtain better water solubility, adhesion, and antibacterial effect, carboxymethyl chitosan with a carboxylation degree of ≥60% is preferably used.

[0014] Preferably, the clay is attapulgite clay.

[0015] Preferably, the molecular weight of the polyethylene glycol is between 200 and 600.

[0016] MS powder generally refers to a mixture containing macroelements, microelements, iron salts, and organic components, and can be purchased commercially.

[0017] In 1000 mL of the wound healer for rubber tree tapping surfaces, the content of carboxymethyl chitosan is, for example, 50 g, 60 g, 70 g, 80 g, 90 g, 100 g, 110 g, 120 g, 130 g, 140 g, 150 g; the content of clay is, for example, 50 g, 60 g, 70 g, 80 g, 90 g, 100 g, 110 g, 120 g, 130 g, 140 g, 150 g, 160 g, 170 g, 180 g, 190 g, 200 g; the content of phytosulfokine is, for example, 0.1 mg, 0.2 mg, 0.4 mg, 0.5 mg, 0.6 mg, 0.8 mg, 1 mg, 1.2 mg; the content of potassium indolebutyrate is, for example, 0.1 g, 0.2 g, 0.3 g, 0.4 g, 0.5 g, 0.6 g, 0.8 g, 0.9 g, 1.0 g; the content of lemon eucalyptus oil is, for example, 0.5 mL, 0.6 mL, 0.8 mL, 1.0 mL, 1.2 mL, 1.4 mL, 1.5 mL; the content of polyethylene glycol is, for example, 0.2 g, 0.3 g, 0.4 g, 0.5 g, 0.6 g, 0.8 g, 0.9 g, 1.0 g; the content of MS powder is, for example, 1 g, 2 g, 3 g, 4 g, 5 g, 6 g, 8 g, 9 g, 10 g; the content of sucrose is, for example, 2 g, 3 g, 4 g, 5 g, 6 g, 8 g, 9 g, 10 g, and the balance is solvent.

[0018] The wound healing agent for cut rubber tree surface of the present invention uses carboxymethyl chitosan as a film-forming agent and clay as a forming agent, and is in a paste state. The wound can be well filled and protected, and is not easy to be lost, not easy to volatilize, and not easy to be washed away by rain. Carboxymethyl chitosan has good water solubility, simple and convenient configuration, and at the same time, carboxymethyl chitosan has good film-forming slow-release property, and has a good slow-release effect on other components in the medicament, thereby improving the utilization rate of the medicament; carboxymethyl chitosan can induce plants to produce broad-spectrum drug resistance, enhance the self-defense ability of plants, and inhibit the growth of various plant pathogenic microorganisms, and has a strong antibacterial effect and good wound healing effect.

[0019] Compared with indolebutyric acid, potassium indolebutyrate is more stable and has longer biological effectiveness; at the same time, its water-soluble properties are more conducive to the configuration of the drug and the absorption of the tree. Potassium indolebutyrate is compounded with plant sulfonyl peptides in this healing agent to form a biological synergistic effect, which is more conducive to wound healing. A certain amount of MS dry powder and sucrose are added to this healing agent to provide a variety of organic matter and nutrients required for wound healing. The composition ratio is relatively reasonable, and the nutrition is rich and comprehensive. Since the healing agent contains certain nutrients and is easy to rot and mold, relatively environmentally friendly and efficient lemon eucalyptus oil is further added. Compared with preservatives such as carbendazim, gentamicin, and dichlorvos, it is safer and more environmentally friendly. Lemon eucalyptus oil also has insect repellent and antioxidant effects, and can delay the aging of rubber trees. Adding a certain amount of polyethylene glycol to this healing agent helps to improve the water-soluble dispersibility of plant sulfonyl peptides and lemon eucalyptus oil, which can not only accelerate the proliferation of wound cells stimulated by plant sulfonyl peptides, but also help the antibacterial effect of lemon eucalyptus oil.

[0020] In a second aspect, the present invention provides a method for preparing a rubber tree cut surface wound healing agent, comprising the following steps:

[0021] (1) Dissolve MS dry powder, sucrose, and potassium indolebutyrate in part of the solvent and fully dissolve;

[0022] (2) adding phytosulfonyl peptide, lemon eucalyptus oil and polyethylene glycol to the solution obtained in step (1) and stirring thoroughly;

[0023] (3) adding carboxymethyl chitosan to the solution obtained in step (2) and obtaining a colloidal solution after the carboxymethyl chitosan is fully dissolved;

[0024] (4) Add clay to the colloidal solution obtained in step (3), stir evenly to obtain a paste mixture, add the remaining solvent, and stir evenly to obtain a paste mixture.

[0025] The method of the present invention does not impose any particular limitation on the amount of solvent (water) used in each step, as long as it can form an ideal solution system with each substance.

[0026] The above preparation method is simple in operation. After dissolving each component separately, according to the characteristics of the solution, the mixing order and addition method are reasonably arranged to form an ideal mixing and blending between the components, so as to ensure that the prepared medicament is evenly dispersed, has ideal stability, and guarantees the application effect after coating.

[0027] In a third aspect, the present invention provides a method for using the above-mentioned wound healing agent for rubber tree tapping surfaces, comprising the following steps:

[0028] After the rubber tree tapping surface is cut, before 9:00 am the next day, remove the dry rubber covering the wound, trim the wound to make the wound edge as smooth as possible. After trimming, apply the healing agent to the wound surface, and the healing agent covers the entire wound.

[0029] Preferably, trim the wound edge with a sharp knife disinfected with alcohol, and trim it according to the specific shape of the wound. On the basis of ensuring that the wound is not enlarged as much as possible, make the wound edge as smooth as possible.

[0030] It is appropriate that the thickness of the healing agent covering the wound is flush with the surrounding tree bark.

[0031] Technical effects:

[0032] The healing agent described in the present invention has good film-forming and sustained-release properties through the mutual cooperation of its components, high absorption and utilization rate of the medicament by the tree body, and the wound healing rate of the rubber tree tapping surface reaches more than 79.20%. The present invention accelerates wound healing, reduces the generation of tree tumors, stabilizes the latex yield of the rubber tree, reduces dead bark, and at the same time increases the utilization rate of the regenerated bark.

[0033] The healing agent described in the present invention is simple to prepare, green and environmentally friendly.

[0034] The present invention has been described in detail above, but the above embodiments are essentially illustrative only and are not intended to limit the present invention. In addition, the present invention is not limited by any theory described in the foregoing prior art or the invention content or the following examples. Description of the drawings

[0035] Figure 1 Typical photos of the wound healing of the tapping surface after 2 months of different treatments. Detailed implementation manners

[0036] The following further illustrates the present invention in conjunction with embodiments. It should be noted that the following embodiments are provided for illustrative purposes only and do not constitute a limitation to the scope of protection required by the present invention.

[0037] Unless otherwise specified, the raw materials, reagents, methods, etc. used in the embodiments are all conventional raw materials, reagents, methods in the art.

[0038] The sources of the raw materials in the embodiments are as follows:

[0039] Carboxymethyl chitosan is N, O-carboxymethyl chitosan with a carboxylation degree of 78%, which is from Zhejiang Jinke Pharmaceutical Co., Ltd.

[0040] The clay is attapulgite clay, which comes from Shijiazhuang Zezhang Mining Co., Ltd.

[0041] Phytosulfopeptides were obtained from Hefei Guopeptide Biotechnology Co., Ltd.

[0042] Potassium indolebutyrate was obtained from Sinopharm Chemical Reagent Co., Ltd.

[0043] Lemon eucalyptus oil comes from Jiangxi Yisenyuan Plant Fragrance Co., Ltd.

[0044] The polyethylene glycol was PEG-400, which was obtained from Sinopharm Chemical Reagent Co., Ltd.

[0045] MS dry powder comes from Sinopharm Chemical Reagent Co., Ltd. and its ingredients include a mixture of macroelements, trace elements, iron salts and organic components.

[0046] Sucrose was from Sinopharm Chemical Reagent Co., Ltd.

[0047] Implementation 1

[0048] Prepare 1L of reagent 1 according to the following components and concentrations:

[0049]

[0050] Preparation method:

[0051] Step 1: Dissolve 4.74g MS dry powder, 6g sucrose, and 0.5g potassium indolebutyrate in 600ml water until fully dissolved;

[0052] Step 2: Add 0.8 mg of phytosulfonyl peptide, 0.75 ml of lemon eucalyptus oil, and 0.67 g of polyethylene glycol to the solution obtained in step 1 in sequence and stir well;

[0053] Step 3: Add 100 g of carboxymethyl chitosan to the solution obtained in step 2, and wait for it to fully dissolve to obtain a colloidal solution;

[0054] Step 4: Add 150g of clay to the colloidal solution obtained in step 3, stir evenly to obtain a paste mixture, continue to add water to make the volume to 1L, stir evenly, and you have it.

[0055] Implementation 2

[0056] Prepare 1L of reagent 2 according to the following components and concentrations:

[0057]

[0058] The preparation method is similar to that of Example 1.

[0059] Example Column 3

[0060] Prepare 1 L of Medicament III according to the following components and concentrations:

[0061]

[0062] The preparation method is similar to that of Example 1.

[0063] Comparative Example 1

[0064] The difference from Example 1 is that it does not contain potassium indolebutyrate.

[0065] Comparative Example 2

[0066] The difference from Example 1 is that it does not contain phyto-sulfokine.

[0067] Comparative Example 3

[0068] The difference from Example 1 is that it does not contain polyethylene glycol.

[0069] Comparative Example 4

[0070] The difference from Example 1 is that it does not contain potassium indolebutyrate and phyto-sulfokine at the same time.

[0071] Comparative Example 5

[0072] The difference from Example 2 is that it does not contain potassium indolebutyrate.

[0073] Comparative Example 6

[0074] The difference from Example 2 is that it does not contain phyto-sulfokine.

[0075] Comparative Example 7

[0076] The difference from Example 2 is that it does not contain polyethylene glycol.

[0077] Comparative Example 8

[0078] The difference from Example 2 is that it does not contain potassium indolebutyrate and phyto-sulfokine at the same time.

[0079] Comparative Example 9

[0080] The difference from Example 3 is that it does not contain potassium indolebutyrate.

[0081] Comparative Example 10

[0082] The difference from Example 3 is that it does not contain phyto-sulfokine.

[0083] Comparative Example 11

[0084] The difference from Example 3 is that it does not contain polyethylene glycol.

[0085] Comparative Example 12

[0086] The difference from Example 3 is that it does not contain potassium indolebutyrate and phytosulfokine at the same time.

[0087] To further verify the application effect of the rubber tree tapping panel wound healer described in the present invention, the present invention further carried out various experimental studies:

[0088] Test Example 1:

[0089] Test variety: Reyan 7-33-97, tapping age 19 years; Method design: This test was set with 7 treatments, 5 replicates, one replicate per tree, for a total of 35 trees. Use a carpenter's knife to dig a wound reaching the xylem (special wound 1.0 cm * 2.0 cm) obliquely along the tapping line above the midpoint of the tapping line.

[0090] Among them:

[0091] Treatment 1: Apply the healer of Example 1;

[0092] Treatment 2: Apply the healer of Comparative Example 1;

[0093] Treatment 3: Apply the healer of Comparative Example 2;

[0094] Treatment 4: Apply the healer of Comparative Example 3;

[0095] Treatment 5: Apply the healer of Comparative Example 4;

[0096] Treatment 6: Apply a commercially available rubber tree tapping panel healing nutrient (this product is produced by Hainan Qianlong Biological Preservation Technology Development Co., Ltd., its main components: sodium alginate, sorbitol, ethanol, sodium benzoate, sodium metasilicate, sodium metabisulfite, boric acid, citric acid);

[0097] Treatment 7: Control, no treatment.

[0098] Method for using the healer: When the tapping panel is cut, before 9 am the next day, remove the dry rubber covering the wound, trim the wound edge with a sharp knife disinfected with alcohol, and trim according to the specific shape of the wound. On the basis of ensuring that the wound is not enlarged as much as possible, make the wound edge as smooth as possible. After trimming, apply the healer on the wound surface, and the healer covers the entire wound, and the thickness should be flush with the surrounding bark.

[0099] Test results: After 2 months of the experiment, the seven treatment groups were investigated, the area of the formed callus was measured, and the rubber tree tapping panel wound healing rate was calculated (healing rate = (wound size before the test - wound size after the test) / wound size before the test), and the results are shown in Table 1.

[0100] The healing rates of the seven treatment groups were as follows: 83.20% (Treatment 1), 57.80% (Treatment 2), 51.20% (Treatment 3), 72.40% (Treatment 4), 44.80% (Treatment 5), 32.20% (Treatment 6), and 25.60% (Treatment 7). Through statistical analysis, significant differences were shown among the treatments (P < 0.05). The healing rate of applying the wound healing agent for rubber trees of the present invention reached 83.20%, which was significantly higher than the 25.60% of the control and the 32.20% of the medicaments sold on the market. Additionally, the healing rates of the treatments without indolebutyric acid (Treatment 2) and without phytosulfokine (Treatment 3) were significantly lower than that of the full formula (Treatment 1), but both were better than the treatment without both indolebutyric acid and phytosulfokine (Treatment 5); the healing rate of Treatment 2 was 13.00% higher than that of Treatment 5, and the healing rate of Treatment 3 was 6.40% higher than that of Treatment 5. However, the healing rate of Treatment 1 was 38.40% higher than that of Treatment 5, which was greater than the sum of the previous two (19.40%). It can be seen that the compounding of potassium indolebutyrate and phytosulfokine can form a biological synergistic effect, which is more conducive to wound healing. The treatment without polyethylene glycol (Treatment 4) was also significantly lower than the full formula treatment (Treatment 1), indicating that the addition of polyethylene glycol significantly improved the healing effect of the healing agent.

[0101] Table 1 Healing rates after 2 months of different treatments

[0102]

[0103]

[0104] Note: The values in the table are mean ± SE (n = 5). Different lowercase letters after the data in the same column indicate significant differences in Duncan's multiple comparisons at the 5% level. The same applies to the following tables.

[0105] Test Example 2:

[0106] Test variety: RRIM600, tapping age 29 years; Method design: This test was set with 7 treatments and 5 replicates, with one replicate for each tree, a total of 35 trees. Use a woodworking knife to dig a wound reaching the xylem along the inclined direction of the tapping line above the midpoint of the tapping line (special wound: 1.0 cm * 2.0 cm).

[0107] Among them:

[0108] Treatment 1: Apply the healing agent of Example 2;

[0109] Treatment 2: Apply the healing agent of Comparative Example 5;

[0110] Treatment 3: Apply the healing agent of Comparative Example 6;

[0111] Treatment 4: Apply the healing agent of Comparative Example 7;

[0112] Treatment Five: Apply the healing agent of Comparative Example 8;

[0113] Treatment Six: Apply the commercially available rubber tree tapping cut surface healing nutrient agent (this product is produced by Hainan Qianlong Biological Preservation Technology Development Co., Ltd., and its main components are: sodium alginate, sorbitol, ethanol, sodium benzoate, sodium metasilicate, sodium metabisulfite, boric acid, citric acid).

[0114] Treatment Seven: Control, without any treatment.

[0115] Usage method of the healing agent: When the tapping cut surface is injured, before 9:00 am the next day, remove the dry rubber covering the wound, trim the wound edge with a sharp knife disinfected with alcohol, and trim according to the specific shape of the wound. On the basis of ensuring that the wound is not enlarged as much as possible, make the wound edge as smooth as possible. After trimming, apply the healing agent on the wound surface, and the healing agent covers the entire wound, and the thickness should be flush with the surrounding bark.

[0116] Test results: After 2 months of the experiment, the seven treatment groups were investigated, the area of the formed callus was measured, and the wound healing rate of the rubber tree tapping cut surface was calculated. The results are shown in Table 2.

[0117] The healing rates of the seven treatment groups are respectively: 80.60% (Treatment One), 58.20% (Treatment Two), 49.80% (Treatment Three), 72.20% (Treatment Four), 43.80% (Treatment Five), 33.60% (Treatment Six), 28.40% (Treatment Seven). Through statistical analysis, it shows that there are significant differences among the treatments (P<0.05). The healing rate of applying the rubber tree wound healing agent of the present invention reaches 80.60%, which is significantly greater than the healing rate of the control of 28.40% and the healing rate of the commercially available agent of 33.60%. In addition, the healing rates of the treatments without adding indolebutyric acid (Treatment Two) and without adding phytosulfokine (Treatment Three) are significantly less than that of the full formula (Treatment One), but are better than the treatment without adding both indolebutyric acid and phytosulfokine (Treatment Five); the healing rate of Treatment Two is 14.40% higher than that of Treatment Five, and the healing rate of Treatment Three is 6.00% higher than that of Treatment Five. However, the healing rate of Treatment One is 36.80% higher than that of Treatment Five, which is greater than the sum of the former two of 20.40%. It can be seen that the compounding of potassium indolebutyrate and phytosulfokine can form a biological synergistic effect, which is more conducive to wound healing. The treatment without adding polyethylene glycol (Treatment Four) is also significantly less than the full formula treatment (Treatment One), indicating that the addition of polyethylene glycol can improve the healing effect of the healing agent.

[0118] Table 2 Healing rates after 2 months of different treatments

[0119]

[0120] Test Example 3:

[0121] Test variety: PR107, tapping age 20 years; Method design: This experiment had 7 treatments, with 5 replicates, one replicate per tree, for a total of 35 trees. Use a woodworking knife to dig a wound reaching the xylem along the inclined direction of the tapping line above the midpoint of the tapping line (special wound: 1.0 cm * 2.0 cm).

[0122] Among them:

[0123] Treatment 1: Apply the healing agent of Example 3;

[0124] Treatment 2: Apply the healing agent of Comparative Example 9;

[0125] Treatment 3: Apply the healing agent of Comparative Example 10;

[0126] Treatment 4: Apply the healing agent of Comparative Example 11;

[0127] Treatment 5: Apply the healing agent of Comparative Example 12;

[0128] Treatment 6: Apply a commercially available rubber tree tapping surface healing nutrient agent (this product is produced by Hainan Qianlong Biological Preservation Technology Development Co., Ltd., its main components: sodium alginate, sorbitol, ethanol, sodium benzoate, sodium metasilicate, sodium metabisulfite, boric acid, citric acid).

[0129] Treatment 7: Control, without any treatment.

[0130] Method of using the healing agent: When the tapping surface is cut, before 9 am the next day, remove the dry rubber covering the wound, trim the wound edge with a sharp knife disinfected with alcohol, and trim according to the specific shape of the wound. On the basis of ensuring that the wound is not enlarged as much as possible, make the wound edge as smooth as possible. After trimming, apply the healing agent to the wound surface, and the healing agent covers the entire wound, with a thickness even with the surrounding bark being appropriate.

[0131] Test results: After 2 months of the experiment, investigate the seven treatment groups, measure the area of the formed callus, and calculate the wound healing rate of the rubber tree tapping surface. The results are shown in Table 3.

[0132] The healing rates of the seven treatment groups were as follows: 79.20% (Treatment 1), 50.40% (Treatment 2), 48.60% (Treatment 3), 69.20% (Treatment 4), 40.00% (Treatment 5), 30.80% (Treatment 6), and 19.40% (Treatment 7). Through statistical analysis, it was shown that there were significant differences among the treatments (P < 0.05). The healing rate of applying the rubber tree wound healer of the present invention reached 79.20%, which was significantly higher than the 19.40% of the control and the 30.80% of the commercially available agents. In addition, the healing rates of the treatments without adding indolebutyric acid (Treatment 2) and without adding phytosulfokine (Treatment 3) were significantly lower than that of the full formula (Treatment 1), but both were better than the treatment without adding both indolebutyric acid and phytosulfokine (Treatment 5); the healing rate of Treatment 2 was 10.40% higher than that of Treatment 5, and the healing rate of Treatment 3 was 8.60% higher than that of Treatment 5. However, the healing rate of Treatment 1 was 39.20% higher than that of Treatment 5, which was greater than the sum of the previous two (19.00%). It can be seen that the compounding of potassium indolebutyrate and phytosulfokine can form a biological synergistic effect, which is more conducive to wound healing. The treatment without adding polyethylene glycol (Treatment 4) was also significantly lower than the full formula treatment (Treatment 1), indicating that the addition of polyethylene glycol significantly improved the healing effect of the healer.

[0133] Table 3 Healing rates after 2 months of different treatments

[0134]

[0135]

[0136] The above test examples illustrate that the agent and the supporting method of the present invention have a good effect on the healing of the rubber tree tapping surface wounds, and among them, the application effect of Example 1 is the best.

[0137] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them. Although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: within the scope of the spirit and essence defined by the claims of the present invention, the technical solutions described in the foregoing embodiments can be modified, or some or all of the technical features can be equivalently replaced; and these modifications or replacements are still within the scope defined by the claims of the present invention.

Claims

1. A wound healing agent for cut surface of rubber tree, characterized in that, Each 1000mL of the rubber tree cut wound healing agent includes the following components: 50-150g of carboxymethyl chitosan, 50-200g of clay, 0.1-1.2mg of plant sulfopeptide, 0.1-1.0g of potassium indolebutyrate, 0.5-1.5ml of lemon eucalyptus oil, 0.2-1.0g of polyethylene glycol, 1.0-10g of MS dry powder, 2-10g of sucrose, and the rest is solvent.

2. The rubber tree cut wound healing agent according to claim 1, characterized in that, Each 1000mL of the rubber tree cut wound healing agent includes the following components: 80-120g of carboxymethyl chitosan, 100-150g of clay, 0.5-1.0mg of plant sulfopeptide, 0.3-0.8g of potassium indolebutyrate, 0.6-1.0ml of lemon eucalyptus oil, 0.3-0.8g of polyethylene glycol, 2.0-6g of MS dry powder, 4-8g of sucrose, and the rest is solvent.

3. The rubber tree cut wound healing agent according to claim 1, characterized in that, Each 1000mL rubber tree cut wound healing agent includes the following components: 100g carboxymethyl chitosan, 150g clay, 0.8mg plant sulfopeptide, 0.5g potassium indolebutyrate, 0.75ml lemon eucalyptus oil, 0.67g polyethylene glycol, 4.74g MS dry powder, 6g sucrose, and the rest is solvent.

4. The rubber tree cut wound healing agent according to any one of claims 1 to 3, characterized in that: The solvent is water.

5. The rubber tree cut wound healing agent according to any one of claims 1 to 3, characterized in that: The carboxymethyl chitosan is one or more of N-carboxymethyl chitosan, O-carboxymethyl chitosan and N,O-carboxymethyl chitosan, and the carboxylation degree of the carboxymethyl chitosan is ≥60%.

6. The rubber tree cut wound healing agent according to any one of claims 1 to 3, characterized in that: The clay is attapulgite clay.

7. The rubber tree cut wound healing agent according to any one of claims 1 to 3, characterized in that: The molecular weight of the polyethylene glycol is between 200 and 600.

8. A method for preparing a rubber tree cut wound healing agent according to any one of claims 1 to 7, characterized in that: The following steps are involved: (1) Dissolve MS dry powder, sucrose, and potassium indolebutyrate in part of the solvent and fully dissolve; (2) adding phytosulfonyl peptide, lemon eucalyptus oil and polyethylene glycol to the solution obtained in step (1) and stirring thoroughly; (3) adding carboxymethyl chitosan to the solution obtained in step (2) and obtaining a colloidal solution after the carboxymethyl chitosan is fully dissolved; (4) Add clay to the colloidal solution obtained in step (3), stir evenly to obtain a paste mixture, add the remaining solvent, and stir evenly to obtain a paste mixture.

9. A method for using the rubber tree cut wound healing agent according to any one of claims 1 to 7, characterized in that: The following steps are involved: After the rubber tree is cut, before 9 am the next day, remove the dry rubber covering the wound and trim the wound to make the wound edge as smooth as possible. After trimming, apply the healing agent to the wound surface so that the healing agent covers the entire wound.

10. The method of use according to claim 9, characterized in that: The healing agent covers the wound to a thickness that is flush with the surrounding bark.

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