Cordyceps sinensis protoplasm freeze-dried powder and preparation method thereof

Abstract

The invention relates to the technical field of cordyceps sinensis food, and discloses cordyceps sinensis protoplasm freeze-dried powder and a preparation method of the cordyceps sinensis protoplasm freeze-dried powder. 10 to 12 parts by weight of lactose; 1 to 2 parts by weight of beta-glucan; 2-3 parts by weight of a ganoderma lucidum extract; 3-5 parts by weight of prebiotics; and 0.5-1 part by weight of a natural antioxidant. The freeze-dried primary pulp disclosed by the invention can effectively preserve active ingredients in fresh cordyceps sinensis fermentation liquor, and has the characteristics of simplicity in preparation, convenience in storage and the like; the cordyceps sinensis oral liquid can preserve the smell and taste of fresh cordyceps sinensis to the greatest extent, and has the advantages of high safety, convenience in taking, high bioavailability and the like; the beta-glucan and the lucid ganoderma extract have a synergistic effect with polysaccharide components in the cordyceps sinensis, so that the immune regulation and antioxidant functions are enhanced.

Description

Technical Field

[0001] The present invention relates to the technical field of Cordyceps sinensis food, and specifically to a Cordyceps sinensis original pulp freeze-dried powder and a preparation method thereof. Background Art

[0002] Cordyceps sinensis is a precious medicinal fungus with various pharmacological effects such as liver and kidney protection, immune regulation, antioxidant, lipid-lowering, and anti-cancer effects. The traditional methods of consuming Cordyceps sinensis (such as eating raw, soaking in water, steaming, etc.) have the following defects: Loss of nutritional components: Drying in the sun or in the shade will cause loss and oxidation of nutritional components.

[0003] Destruction of active ingredients: Heating above 60°C will destroy macromolecular polysaccharides and volatile substances, reducing the efficacy.

[0004] Low bioavailability: Polysaccharide components are insoluble in water, resulting in low bioavailability.

[0005] In recent years, the demand for the pharmacological application of Cordyceps sinensis has been increasing continuously, but its traditional processing methods have restricted its industrial development.

[0006] Therefore, the present invention provides a Cordyceps sinensis original pulp freeze-dried powder and a preparation method thereof. Summary of the Invention

[0007] The purpose of the present invention is to solve at least one technical problem raised in the background art.

[0008] On the one hand, the embodiments of the present application provide a Cordyceps sinensis original pulp freeze-dried powder, and the raw materials of the freeze-dried powder include the following components in parts by weight: Cordyceps sinensis: 4 - 6 parts by weight; Lactose: 10 - 12 parts by weight; β-glucan: 1 - 2 parts by weight; Ganoderma lucidum extract: 2 - 3 parts by weight; Prebiotic: 3 - 5 parts by weight; Natural antioxidant: 0.5 - 1 part by weight.

[0009] Further, the prebiotic is fructooligosaccharide or inulin.

[0010] Further, the natural antioxidant is green tea extract or vitamin C.

[0011] On the other hand, the embodiments of the present application provide a preparation method of a Cordyceps sinensis original pulp freeze-dried powder, including the following steps: S1: Dissolve β-glucan, Ganoderma lucidum extract, prebiotic and natural antioxidant in water respectively to form a uniform solution; S2: Mix cordyceps sinensis, lactose with the above solution, and perform tissue disruption to obtain a mixture. S3: Filter the mixture through a sieve to obtain a preliminary filtrate. S4: Filter the preliminary filtrate through a filter membrane to obtain a refined filtrate. S5: Fill the refined filtrate into a freeze-drying container. S6: Perform freeze-drying on the filled cordyceps sinensis raw pulp to obtain a finished freeze-dried powder.

[0012] Further, the tissue disruption is carried out using a high-speed homogenizer or an ultrasonic crusher, and the disruption time is 5 - 10 minutes.

[0013] Further, the aperture of the sieve is 100 - 200 mesh, which is used to remove large particle impurities.

[0014] Further, the aperture of the filter membrane is 0.22 - 0.45 μm, which is used to remove microorganisms and fine particles.

[0015] Further, the process conditions for the freeze-drying are as follows: Pre-freezing temperature: -40°C to -50°C, pre-freezing time: 2 - 4 hours; Sublimation drying temperature: -20°C to -30°C, sublimation drying time: 10 - 15 hours; Desorption drying temperature: 20°C to 30°C, desorption drying time: 4 - 6 hours.

[0016] Further, the water content of the freeze-dried powder does not exceed 5%.

[0017] Compared with the prior art, the beneficial effects of the technical solution of this application are as follows: 1. The nutritional components of fresh cordyceps sinensis are more abundant than those of dried cordyceps sinensis. Through research, comparison and detection, it is found that the contents of protein polypeptides, trace elements, polysaccharides, etc. in fresh cordyceps sinensis are significantly higher than those of the same dried cordyceps sinensis. Moreover, when taken fresh, without going through high temperature, the nutritional components are not lost and are more easily absorbed by the human body. The freeze-dried raw pulp of the present invention can effectively preserve the active components in the fresh cordyceps sinensis fermentation broth, and has the characteristics of simple preparation and convenient storage; 2. The present invention can preserve the smell and taste of fresh cordyceps sinensis to the greatest extent, and has the advantages of high safety, convenient administration, high biological utilization rate, etc.

[0018] 3. β-glucan and ganoderma extract act synergistically with the polysaccharide components in cordyceps sinensis to enhance the immune regulation and antioxidant functions.

[0019] 4. Natural antioxidants effectively prevent the oxidation of active components and extend the shelf life of the product.

[0020] The present invention not only retains the traditional efficacy of Cordyceps sinensis, but also has multiple functions such as enhancing immunity, improving intestinal health and antioxidant properties. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Other features, objects and advantages of the present invention will become more apparent by reading the following detailed description of non-limiting embodiments with reference to the accompanying drawings: Figure 1 It is a flowchart of the preparation method of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0022] The present invention will be further described below in conjunction with the drawings and embodiments, but it shall not be used as a basis for limiting the present invention.

[0023] As Figure 1 shown, Example 1: A freeze-dried powder of Cordyceps sinensis raw pulp, the raw materials of the freeze-dried powder include the following components in parts by weight: Cordyceps sinensis: 5 parts by weight; Lactose: 11 parts by weight; β-glucan: 1.5 parts by weight; Ganoderma lucidum extract: 2.5 parts by weight; Fructooligosaccharide: 4 parts by weight; Green tea extract: 0.8 parts by weight.

[0024] The preparation steps of a freeze-dried powder of Cordyceps sinensis raw pulp are as follows: Dissolve β-glucan, Ganoderma lucidum extract, fructooligosaccharide and green tea extract in water respectively to form a uniform solution; Mix Cordyceps sinensis, lactose with the above solution, and use a high-speed homogenizer to crush for 8 minutes to obtain a mixture; Filter the mixture with a 150-mesh sieve to obtain a preliminary filtrate; Filter the preliminary filtrate with a 0.45 μm filter membrane to obtain a refined filtrate; Fill the refined filtrate into a freeze-drying container; Perform freeze-drying: Pre-freezing: -45 °C, 3 hours; Sublimation drying: -25 °C, 12 hours; Desorption drying: 25 °C, 5 hours.

[0025] Obtain a freeze-dried powder of Cordyceps sinensis raw pulp with a water content not exceeding 5%.

[0026] Example 2: The raw material ratio of the freeze-dried powder with different β-glucan contents: Cordyceps sinensis: 5 parts by weight; Lactose: 11 parts by weight; β-glucan: 1 part by weight (Example 2A) or 2 parts by weight (Example 2B); Ganoderma lucidum extract: 2.5 parts by weight; Fructooligosaccharide: 4 parts by weight; Green tea extract: 0.8 part by weight.

[0027] Preparation steps: Same as Example 1.

[0028] Test results: Example 2A (β-glucan 1 part by weight): The immunomodulatory function is improved by 15%; Example 2B (β-glucan 2 parts by weight): The immunomodulatory function is improved by 25%.

[0029] Example 3: Raw material ratio for preparing freeze-dried powder with different prebiotics: Cordyceps sinensis: 5 parts by weight; Lactose: 11 parts by weight; β-glucan: 1.5 parts by weight; Ganoderma lucidum extract: 2.5 parts by weight; Prebiotic: Fructooligosaccharide 4 parts by weight (Example 3A) or inulin 4 parts by weight (Example 3B); Green tea extract: 0.8 part by weight.

[0030] Preparation steps: Same as Example 1.

[0031] Test results: Example 3A (fructooligosaccharide): The proliferation effect of intestinal probiotics is significant; Example 3B (inulin): The proliferation effect of intestinal probiotics is more significant, and the taste of the freeze-dried powder is improved.

[0032] Example 4: Raw material ratio for preparing freeze-dried powder with different natural antioxidants: Cordyceps sinensis: 5 parts by weight; Lactose: 11 parts by weight; β-glucan: 1.5 parts by weight; Ganoderma lucidum extract: 2.5 parts by weight; Fructooligosaccharide: 4 parts by weight; Natural antioxidant: Green tea extract 0.8 part by weight (Example 4A) or vitamin C 0.8 part by weight (Example 4B).

[0033] Preparation steps: Same as Example 1.

[0034] Test results: Example 4A (green tea extract): The antioxidant capacity is improved by 20%; Example 4B (Vitamin C): The antioxidant capacity is increased by 25%, and the product has better color.

[0035] Example 5: Raw material ratio for preparing freeze-dried powder with different Ganoderma lucidum extract contents: Cordyceps sinensis: 5 parts by weight; Lactose: 11 parts by weight; β-glucan: 1.5 parts by weight; Ganoderma lucidum extract: 2 parts by weight (Example 5A) or 3 parts by weight (Example 5B); Fructooligosaccharide: 4 parts by weight; Green tea extract: 0.8 parts by weight.

[0036] Preparation steps: Same as Example 1.

[0037] Test results: Example 5A (2 parts by weight of Ganoderma lucidum extract): The immunomodulatory function is increased by 20%; Example 5B (3 parts by weight of Ganoderma lucidum extract): The immunomodulatory function is increased by 30%.

[0038] Example 6: Raw material ratio for preparing freeze-dried powder with different freeze-drying processes: Same as Example 1.

[0039] Preparation steps: Example 6A: Pre-freezing at -40°C, sublimation drying at -20°C, and desorption drying at 20°C; Example 6B: Pre-freezing at -50°C, sublimation drying at -30°C, and desorption drying at 30°C.

[0040] Test results: Example 6A: The water content of the freeze-dried powder is 4.5%, and the preservation rate of active ingredients is 95%; Example 6B: The water content of the freeze-dried powder is 3.8%, and the preservation rate of active ingredients is 98%.

[0041] Example 7: Functional verification of the improved freeze-dried powder - immunomodulatory function: Verified by cell experiments, the improved freeze-dried powder significantly enhanced the phagocytic activity of macrophages.

[0042] Antioxidant capacity: Verified by DPPH free radical scavenging experiment, the antioxidant capacity of the improved freeze-dried powder is increased by more than 20%.

[0043] Intestinal probiotic proliferation effect: Verified by in vitro fermentation experiment, the improved freeze-dried powder significantly promoted the proliferation of Bifidobacterium and Lactobacillus.

[0044] Example 8: Stability Test of Improved Lyophilized Powder Test Conditions: Place the lyophilized powder in an environment of 25°C and 60% relative humidity and store it for 6 months.

[0045] Test Results: The retention rate of the active ingredients (nucleosides, polysaccharides, sterols) exceeds 90%; There are no obvious changes in the color, smell and taste of the product.

[0046] The above are only the preferred embodiments of the present application and are not used to limit the present application. For those skilled in the art, various changes and modifications can be made to the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A Cordyceps sinensis slurry freeze-dried powder, characterized in that: The raw materials of the freeze-dried powder include the following components in parts by weight: Cordyceps sinensis: 4-6 parts by weight; Lactose: 10-12 parts by weight; β-glucan: 1-2 parts by weight; Ganoderma lucidum extract: 2-3 parts by weight; Prebiotics: 3-5 parts by weight; Natural antioxidant: 0.5-1 parts by weight.

2. The Cordyceps sinensis slurry freeze-dried powder according to claim 1, characterized in that The prebiotics are oligofructose or inulin.

3. The Cordyceps sinensis slurry freeze-dried powder according to claim 1, characterized in that The natural antioxidant is green tea extract or vitamin C.

4. A method for preparing a freeze-dried powder of Cordyceps sinensis pulp according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1: dissolving β-glucan, ganoderma lucidum extract, prebiotics and natural antioxidants in water to form a uniform solution; S2: mixing Cordyceps sinensis, lactose and the above solution, breaking the tissues to obtain a mixed solution; S3: filtering the mixed solution through a sieve to obtain a primary filtrate; S4: filtering the primary filtrate through a filter membrane to obtain a refined filtrate; S5: filling the refined filtrate into a freeze-drying container; S6: freeze-drying the filled Cordyceps sinensis slurry to obtain a finished freeze-dried powder.

5. The method for preparing freeze-dried powder of Cordyceps sinensis pulp according to claim 4, characterized in that: The tissue is crushed using a high-speed homogenizer or an ultrasonic crusher, and the crushing time is 5-10 minutes.

6. The method for preparing freeze-dried powder of Cordyceps sinensis pulp according to claim 4, characterized in that: The sieve has an aperture of 100-200 meshes and is used to remove large particle impurities.

7. The method for preparing freeze-dried powder of Cordyceps sinensis pulp according to claim 4, characterized in that: The pore size of the filter membrane is 0.22-0.45 μm, and is used to remove microorganisms and tiny particles.

8. The method for preparing freeze-dried powder of Cordyceps sinensis pulp according to claim 4, characterized in that: The freeze-drying process conditions are: Pre-freezing temperature: -40℃ to -50℃, pre-freezing time: 2-4 hours; Sublimation drying temperature: -20℃ to -30℃, sublimation drying time: 10-15 hours; Drying temperature: 20℃ to 30℃, drying time: 4-6 hours.

9. The method for preparing freeze-dried powder of Cordyceps sinensis pulp according to claim 4, characterized in that: The water content of the lyophilized powder does not exceed 5%.

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