Sparassis crispa American ginseng paste and preparation method thereof

Abstract

The invention discloses sparassis crispa American ginseng paste and a preparation method thereof. The sparassis crispa American ginseng paste is prepared from the following components in percentage by weight: 25 to 35 percent of sparassis crispa, 20 to 30 percent of radix astragali seu hedysari, 15 to 25 percent of American ginseng, 10 to 20 percent of radix codonopsis and 0 to 15 percent of flavoring agent. The traditional Chinese medicine composition has the effects of benefiting qi, nourishing blood and enhancing immunity.

Description

Technical Field

[0001] The present invention relates to the technical field of food processing, and particularly relates to a Sparassis crispa and American ginseng paste and a preparation method thereof. Background Art

[0002] Extract paste is a semi-solid preparation made by decocting traditional Chinese medicine pieces multiple times, filtering the residue and taking the juice for concentration, and then adding auxiliary materials such as honey, sugar or donkey-hide gelatin. It belongs to one of the 8 classic dosage forms in traditional Chinese medicine, namely "pills, powders, extracts, pills, decoctions, dew, wines, and lozenges".

[0003] Chinese patent document CN111870630A discloses a drug formula and preparation process for enhancing resistance and immunity, including astragalus root, American ginseng, schisandra fruit, codonopsis pilosula, chrysanthemum, heterophylly falsestarwort root, and poria cocos. It has the effects of improving immunity and resistance, promoting blood circulation and enhancing physical fitness. This is mainly because among the components of this invention, astragalus root and codonopsis pilosula both enter the lung meridian in traditional Chinese medicine and have the effect of replenishing qi. Both drugs have the effects of tonifying the spleen and lung, replenishing qi and promoting fluid production, and replenishing qi to generate blood. At the same time, heterophylly falsestarwort root has the ability to enhance immune function. When taking the drug, astragalus root and codonopsis pilosula promote blood circulation so that the body can better absorb the drug. At the same time, American ginseng and schisandra fruit can excite the nerves and resist fatigue, effectively regulating and improving the functions of the human body. Chrysanthemum plays a role in dilating coronary arteries, increasing coronary blood flow, and increasing myocardial oxygen consumption, which can further enhance the blood circulation of the body and make the medicinal effect better exerted with the acceleration of blood flow.

[0004] Chinese patent document CN103381249A discloses a preparation process of a cordyceps sinensis and American ginseng preparation for enhancing immunity and its capsule. The weight parts of each component of the cordyceps sinensis and American ginseng preparation are as follows: 240 - 260 parts of American ginseng, 95 - 110 parts of cordyceps militaris mycelium, 140 - 160 parts of astragalus root, 90 - 110 parts of angelica sinensis, 180 - 220 parts of coix seed, 90 - 120 parts of heterophylly falsestarwort root, 90 - 120 parts of codonopsis pilosula, 80 - 100 parts of hawthorn fruit, 75 - 85 parts of dried tangerine peel, 90 - 120 parts of Chinese date, and 55 - 65 parts of hyacinth bean. Starting from the integrity of the basic theory of traditional Chinese medicine, this invention exerts the effects of tonifying the deficiency of qi, blood, yin, and yang of the five zang-organs of the lung, spleen, heart, liver, and kidney by tonifying the deficiency of qi in the heart and lungs, warming and invigorating the spleen yang, strengthening the spleen and promoting digestion, and nourishing the liver and kidneys, so as to achieve the effects of anti-fatigue and improving the body's immune function. This invention improves the medication conditions, the preparation process is simple and feasible, the dosage is small, convenient, and the curative effect is stable, remarkable, and has no side effects.

[0005] After retrieval, although the prior art discloses the compounding of astragalus root, American ginseng, and codonopsis pilosula. However, in the prior art, there is no discovery of the compounding of Sparassis crispa with astragalus root, American ginseng, and codonopsis pilosula, and the prior art has not found that the compounding of Sparassis crispa, astragalus root, American ginseng, and codonopsis pilosula has a synergistic effect in enhancing the immune regulation function. Summary of the Invention

[0006] In view of this, the purpose of the present invention is to provide a Sparassis crispa, American ginseng paste and its preparation method. By compounding Sparassis crispa, Astragalus membranaceus, American ginseng, and Codonopsis pilosula, the immune regulation function can be synergistically enhanced, and the taste can be improved by using flavoring agents and the prebiotic function can be utilized.

[0007] The technical solution adopted is as follows:

[0008] A Sparassis crispa, American ginseng paste of the present invention is composed of the following components in weight ratio: Sparassis crispa 25 - 35%, Astragalus membranaceus 20 - 30%, American ginseng 15 - 25%, Codonopsis pilosula 10 - 20%, flavoring agent 0 - 15%.

[0009] Further, the flavoring agent is isomaltooligosaccharide syrup.

[0010] Further, the Sparassis crispa is the dried fruiting body extracted by CO 2 supercritical extraction, and the content of β-glucan is ≥ 35%.

[0011] Further, the DE value of the isomaltooligosaccharide syrup is ≤ 10%, and it contains one or several of isomaltose, panose, and isomaltotriose.

[0012] Further, the flavoring agent is 5 - 15%.

[0013] Further, the dosage form of the Sparassis crispa, American ginseng paste is paste extract, and the density is 1.2 - 1.3 g / cm 3 , and the pH value is 5.0 - 6.0.

[0014] A preparation method of the Sparassis crispa, American ginseng paste of the present invention includes the following steps:

[0015] S1. Raw material pretreatment: Wash and drain the Sparassis crispa, and cut it into slices with a thickness of 3 - 5 mm; Wash, pulverize Astragalus membranaceus, American ginseng, and Codonopsis pilosula to 60 - 80 meshes respectively;

[0016] S2. Water extraction and concentration: Mix the raw materials in proportion, add 8 - 10 times the weight of water, heat at a temperature of 98°C ± 1°C, and decoct for 1 - 2 hours; After filtering out the medicinal liquid, add 5 - 7 times the weight of water to the medicinal residues, heat at a temperature of 95°C ± 1°C, and the time is 0.5 - 1.5 hours; Combine the filtrates and concentrate under reduced pressure to a clear paste with a relative density of 1.10 - 1.15, wherein the temperature of concentration under reduced pressure is 60°C ± 2°C; The pressure of concentration under reduced pressure is -0.08 to -0.10 MPa;

[0017] S3. Mixing and blending: Add isomaltooligosaccharide syrup, and stir and mix at 80 - 85°C for 30 - 60 minutes;

[0018] S4. Sterilization and filling: Pasteurize and fill while it is hot into a sterile container to obtain Sparassis crispa and American ginseng paste.

[0019] In the above technical solution,

[0020] "Sparassis crispa" is a variety cultivated by Hangzhou Qiandao Xiugu Biotechnology Co., Ltd. under the guidance of the edible and medicinal mushroom team of the Horticultural Research Institute of Zhejiang Academy of Agricultural Sciences, and it is the main Sparassis crispa cultivation variety in Zhejiang Province.

[0021] The fruiting body of "Sparassis crispa" is medium to large in size, fleshy, with many branches growing from a thick stalk base, and the ends of the branches form a huge hydrangea composed of many zigzag lobes, which is more compact, whiter in color, and more uniform in shape than the fruiting bodies of ordinary Sparassis crispa.

[0022] Sparassis crispa is the dried fruiting body extracted by supercritical CO 2 with a β-glucan content of ≥ 35%.

[0023] In the present invention, by compounding Sparassis crispa, Astragalus membranaceus, American ginseng, and Codonopsis pilosula, the immune regulation function is synergistically enhanced, and the taste and prebiotic function can be improved by using a flavoring agent. For example, in the present invention, Sparassis crispa + Astragalus membranaceus form a synergistic combination of β-glucan and astragaloside IV, and isomaltooligosaccharide syrup can be added, which serves as both a flavoring agent and inhibits microorganisms through its low water activity.

[0024] The present invention has the effects of invigorating qi and nourishing blood, and enhancing immunity, and adopts a gradient temperature control (98°C ± 1°C → 95°C ± 1°C) decoction combined with vacuum concentration (-0.08 to -0.10 MPa, 60°C ± 2°C) process, which improves the saponin retention rate to 92.3% and extends the shelf life to 18 months. Brief Description of the Drawings

[0025] The following is a brief description of the drawings:

[0026] Figure 1 It is a phenotype diagram of the number of zebrafish red blood cells (staining intensity).

[0027] Figure 2 It is a phenotype diagram of zebrafish T cells.

[0028] Figure 3 It is a certificate diagram issued by Tongbiao Testing and Certification (Jiangsu) Co., Ltd. through Tongbiao animal experiments, proving that the Sparassis crispa and American ginseng paste of the present invention has the effects of invigorating qi and nourishing blood, and enhancing immunity on living animals.

[0029] Figures 4 - 8 It is an excerpt of the test report issued by Tongbiao Testing and Certification (Jiangsu) Co., Ltd. Figures 1 - 5 . Detailed Description of the Invention

[0030] The present invention will be described in detail below through specific embodiments. However, the uses and purposes of these exemplary embodiments are only used to illustrate the present invention, and do not constitute any form of limitation to the actual protection scope of the present invention, nor will the protection scope of the present invention be limited thereto.

[0031] Example 1

[0032] A Sparassis crispa and American ginseng paste in this example is composed of the following components by weight ratio: 30 kg of Sparassis crispa, 25 kg of Astragalus membranaceus, 20 kg of American ginseng, 15 kg of Codonopsis pilosula, and 10 kg of flavoring agent.

[0033] The Sparassis crispa is the dried fruiting body extracted by supercritical CO 2 supercritical extraction, in which the content of β-glucan is 47.1%, which is higher than that of general Sparassis crispa.

[0034] The preparation method of the Sparassis crispa and American ginseng paste includes the following steps:

[0035] (1) Pretreatment of raw materials:

[0036] Sparassis crispa: Take the dried product, remove impurities, wash it twice with pure water, drain it, and cut it into pieces with a thickness of 3-5 mm.

[0037] Astragalus membranaceus, American ginseng, Codonopsis pilosula: Select the main roots of medicinal materials with a diameter ≥ 1 cm. After screening by an X-ray foreign matter detector, crush them to 60 mesh (particle size ≤ 250 μm) with an ultrafine pulverizer.

[0038] (2) Water extraction and concentration:

[0039] First decoction: Put the pretreated mixed raw materials (30 kg of Sparassis crispa + 25 kg of Astragalus membranaceus + 20 kg of American ginseng + 15 kg of Codonopsis pilosula) into a multi-functional extraction tank, add 800 L of deionized water (weight ratio of material to liquid 1:8), turn on the steam to heat to 98°C ± 1°C, and keep stirring (rotation speed 45 r / min) for 1.5 hours.

[0040] Second decoction: After filtering out the medicinal liquid, add 600 L of water (material to liquid ratio 1:6) to the medicinal residues, and decoct at 95°C ± 1°C for 1 hour.

[0041] Combine the two filtrates, filter through a 200-mesh sieve, transfer to a vacuum concentration tank, control the vacuum degree at -0.09 MPa, and the temperature at 60°C ± 2°C, and concentrate to a relative density of 1.12 (measured at 20°C).

[0042] (3) Mixing and blending:

[0043] Slowly add 10 kg of isomaltooligosaccharide syrup (solid content 75%) to the concentrated solution, turn on the shear emulsifier (rotation speed 3000 rpm) and stir for 30 minutes until the paste is uniform and free of particles.

[0044] (4) Sterilization and filling:

[0045] Use a tubular pasteurizer, set the temperature at 85°C ± 2°C, the flow rate at 500 L / h, and immediately fill it into brown glass bottles (previously sterilized by dry heat at 180°C for 2 hours) after sterilization, and fill with nitrogen and seal.

[0046] I. Conduct performance tests on the Sparassis crispa and American ginseng paste of Example 1, and the test results are shown in Table 1.

[0047] Table 1

[0048]

[0049] II. Efficacy tests on the Sparassis crispa and American ginseng paste of Example 1

[0050] (1). Tonifying qi and nourishing blood

[0051] 1.1 Evaluation principle

[0052] This experiment aims to evaluate the efficacy of Sparassis crispa and American ginseng paste in tonifying qi and nourishing blood using zebrafish as a model.

[0053] 1.2 Experimental materials and methods

[0054] (1) Experimental animals: Randomly select 90 wild-type AB strain zebrafish at 2 dpf and place them in a 6-well plate, with 30 tails in each well.

[0055] (2) Grouping settings: Randomly divide the zebrafish into a normal control group, a model control group, and an experimental group, with an equal number in each group.

[0056] (3) Model construction: Establish a zebrafish anemia model induced by phenylhydrazine.

[0057] (4) Treatment intervention: The experimental group was respectively given Sparassis crispa and American ginseng paste. At the same time, a normal control group and a model control group were set up, with a volume of 3 mL in each well. After treating the zebrafish with Sparassis crispa and American ginseng paste and phenylhydrazine for 18 hours, stain with o-dianisidine. After staining, randomly select 10 zebrafish from each experimental group and take pictures and collect data under a dissection microscope, and analyze and statistically analyze the staining intensity S of cardiac red blood cells to evaluate the improvement effect of Sparassis crispa and American ginseng paste.

[0058] (5) Observation index: Observe the average staining intensity of cardiac red blood cells in zebrafish under a fluorescence microscope and take pictures.

[0059] 1.3 Experimental results

[0060] As shown in the results in Table 1, the staining intensity of red blood cells in the hearts of zebrafish in the model control group was significantly decreased compared with that in the normal control group (P < 0.001), indicating that the model was successfully established. The staining intensity of red blood cells in the hearts of zebrafish in the Sparassis crispa and American ginseng cream group was 4010. The results showed that under the experimental conditions of this study, Sparassis crispa and American ginseng cream had an obvious improvement effect on phenylhydrazine-induced anemia in zebrafish. Figure 1 It is a representative picture of red blood cell staining in the hearts of zebrafish in each experimental group, and the results are consistent with the above description.

[0061] Table 2 Improvement effect of Sparassis crispa and American ginseng cream on anemic zebrafish

[0062] Group Heart red blood cell staining intensity (pixels) Normal control group 4263±162 Model control group 2183±59 Sparassis crispa and American ginseng paste 4010±100***

[0063] Compared with the model control group, ***P < 0.001; n = 10

[0064] See Figure 1 The phenotypic map of the number (staining intensity) of zebrafish red blood cells shown in the figure. The red dotted area is the heart, and the red inside the heart is red blood cells. It can be seen that the number (staining intensity) of red blood cells in the experimental group treated with Sparassis crispa and American ginseng cream is similar to that in the normal control group without phenylhydrazine intake, and there is no obvious decrease in red blood cells. However, the number (staining intensity) of red blood cells in the experimental group is significantly increased compared with the model control group.

[0065]

Evaluation conclusion

[0066] 1. Through the comparative experiment of 30 zebrafish in each group, the number (staining intensity) of red blood cells in the hearts of the Sparassis crispa and American ginseng cream group is similar to that in the normal control group without phenylhydrazine intake, and there is no decrease in the number of red blood cells in the hearts of the model control group.

[0067] 2. This experiment confirmed that Sparassis crispa and American ginseng cream have the effect of significantly replenishing qi and nourishing blood.

[0068] (2). Enhance immunity

[0069] 2.1 Evaluation principle

[0070] Zebrafish with immunosuppression model was established by intravenous injection of vinorelbine. High-dose vinorelbine has obvious bone marrow suppression, resulting in a decrease in the number of platelets, red blood cells and white blood cells (neutrophils, macrophages, T cells, etc.) and anemia, and ultimately leading to immunosuppression. Humans and zebrafish are extremely similar in the cell composition of the immune system, and zebrafish is the smallest in volume among all animal individuals with both specific immunity and non-specific immunity at present, which is suitable for high-throughput evaluation of immunomodulatory efficacy. Immunosuppression can be caused by intravenous injection of high-dose vinorelbine in zebrafish.

[0071] Using genetically modified T-cell red fluorescent zebrafish, it can be observed under a fluorescence microscope that the fluorescence intensity of T cells in zebrafish with low immunity is significantly weakened.

[0072] 2.2 Test protocol

[0073] We divided the tested zebrafish into three groups: a normal control group, a model control group, and a group taking an immunomodulator (Sparassis crispa and American ginseng paste). The normal control group was not injected with vinorelbine, while both the model control group and the group taking the immunomodulator were injected with an equal amount of vinorelbine (vinorelbine was introduced into the zebrafish body by intravenous injection). The group taking the immunomodulator ingested Sparassis crispa and American ginseng paste after injecting vinorelbine.

[0074] After taking the immunomodulator for a period of time, we observed the changes in the fluorescence intensity of zebrafish T cells.

[0075] 2.3 Test results

[0076] See Figure 2 the zebrafish T cell phenotype diagram shown, where the red fluorescent particles indicated by the blue arrow are T cells. It can be seen that the fluorescence intensity of T cells in the immunomodulator group (Sparassis crispa and American ginseng paste) is similar to that of the normal control group without vinorelbine injection, and there is no obvious reduction in T cells.

[0077] 2.4 Evaluation conclusion

[0078] 1. After the comparative experiment with 30 zebrafish in each group, the fluorescence intensity of T cells in the immunomodulator group (Sparassis crispa and American ginseng paste) is similar to that of the normal control group, and there is no reduction in T cells in the model control group.

[0079] 2. This experiment confirmed that Sparassis crispa and American ginseng paste has the effect of enhancing immunity.

[0080] It should be noted that Figure 3 is the certificate diagram issued by Tongbiao Testing and Certification (Jiangsu) Co., Ltd. through Tongbiao animal experiments, which confirmed that the Sparassis crispa and American ginseng paste of the present invention has the effects of supplementing qi and nourishing blood and enhancing immunity on living animals.

[0081] Figures 4 - 8 is the excerpt of the test report issued by Tongbiao Testing and Certification (Jiangsu) Co., Ltd. Figures 1 - 6 .

[0082] The series of detailed descriptions listed above are only specific descriptions of the feasible embodiments of the present invention, and they are not intended to limit the protection scope of the present invention. Any equivalent embodiments or modifications made without departing from the technical spirit of the present invention should be included within the protection scope of the present invention.

Claims

1. A flower ear hydrangea and American ginseng paste, characterized in that: The invention is composed of the following components in weight ratio: 25-35% of Hydrangea auricula, 20-30% of Astragalus, 15-25% of American ginseng, 10-20% of Codonopsis pilosula and 0-15% of flavor corrective.

2. The hydrangea auricula and American ginseng paste according to claim 1, characterized in that The flavoring agent is isomaltooligosaccharide syrup.

3. The hydrangea auricula and American ginseng paste according to claim 1, characterized in that: The hydrangea auricula is a dry fruiting body extracted by CO2 supercritical extraction, wherein the content of β-glucan is ≥35%.

4. The hydrangea auricula and American ginseng paste according to claim 1, characterized in that: The DE value of the isomaltooligosaccharide syrup is ≤10%, and contains one or more of isomaltose, panose and isomaltotriose.

5. The hydrangea auricula and American ginseng paste according to claim 1, characterized in that: The flavoring agent is 5-15%.

6. The hydrangea auricula and American ginseng paste according to claim 1, characterized in that: The dosage form of the flower ear hydrangea and American ginseng paste is a paste, and the density is 1.2-1.3g / cm 3 , pH value is 5.0-6.

0.

7. A method for preparing the hydrangea auricula-American ginseng paste according to any one of claims 1 to 6, characterized in that: The steps include: S1. Raw material pretreatment: Wash and drain the Hydrangea auricula and cut into 3-5 mm slices; wash and crush Astragalus, American ginseng and Codonopsis pilosula to 60-80 mesh; S2. Water extraction and concentration: Mix the raw materials in proportion, add 8-10 times the weight of water, heat to 98℃±1℃, and boil for 1-2 hours; after filtering out the liquid medicine, add 5-7 times the weight of water to the residue, heat to 95℃±1℃, and cook for 0.5-1.5 hours; combine the filtrate and concentrate under reduced pressure to a clear paste with a relative density of 1.10-1.15, wherein the temperature of reduced pressure concentration is 60℃±2℃; the pressure of reduced pressure concentration is -0.08~-0.10MPa; S3. Mixing: Add isomaltooligosaccharide syrup and stir at 80-85°C for 30-60 minutes; S4. Sterilization and filling: pasteurization, filling into sterile containers while hot, to obtain the Hydrangea odorata and American ginseng paste.

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