Sterilizing wet tissue and manufacturing process thereof

Abstract

The invention belongs to the technical field of wet tissues, and particularly relates to a sterilizing wet tissue and a manufacturing process thereof. The sterilizing wet tissue comprises a wet tissue base material and a wet tissue soaking solution, the wet tissue soaking solution comprises the following components in parts by weight: 10-15 parts of a sterilizing component; 0.5-1 part of plant essential oil; 90 to 100 parts of water; the bactericidal component is prepared from modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of (1-2): 1; the sterilization rate of the sterilization wet tissue to staphylococcus aureus, escherichia coli, candida albicans and bacillus subtilis in a short time reaches 98% or above, the sterilization performance is excellent, meanwhile, the good moisturizing effect is achieved, skin irritation is avoided, and safety is high.

Description

Technical Field

[0001] The present invention belongs to the technical field of wet wipes, and particularly relates to a bactericidal wet wipe and its manufacturing process. Background Art

[0002] Wet wipes are a commonly used personal cleaning product, usually made of non-woven fabric, paper towels or cotton cloth, and contain a certain amount of moisture, moisturizing agents, and bactericidal or disinfectant or skin care ingredients, and are widely used in daily life; common types of wet wipes are mainly divided into ordinary cleaning wet wipes, disinfection wet wipes, baby wet wipes, and makeup remover wet wipes. Among them, ordinary cleaning wet wipes are mainly used for daily hand, face or object surface cleaning, usually containing mild surfactants, which can effectively remove stains and grease; disinfection wet wipes, in addition to having a cleaning function, also add disinfectants such as alcohol, quaternary ammonium salts, etc., which can kill or inhibit the growth of microorganisms such as bacteria and viruses; baby wet wipes are mainly used for daily cleaning of babies, such as wiping the buttocks, hands, etc., usually do not contain irritating ingredients such as alcohol and fragrances, and add skin moisturizing ingredients such as glycerin and aloe vera; makeup remover wet wipes contain makeup remover ingredients such as surfactants and solvents, which can easily remove makeup and are suitable for quick makeup removal or use during travel; with the continuous improvement of people's living standards, the functional requirements for wet wipes are also getting higher and higher, and functional wet wipes with bactericidal, moisturizing, and low irritation functions have gradually become the focus of attention.

[0003] Chinese Patent Application No. CN202410399749.1 discloses a disinfection and sterilization wet wipe and its preparation method. The disinfection and sterilization wet wipe contains a disinfection and sterilization agent, and the disinfection and sterilization agent includes 0.4 - 2.4 wt% of lactic acid and 0.02 - 0.1 wt% of chlorhexidine compounds. By adding lactic acid and chlorhexidine compounds into water and stirring evenly, the disinfection and sterilization agent is obtained, and then the non-woven fabric is soaked in the disinfection and sterilization agent to obtain the disinfection and sterilization wet wipe; although the two components can synergistically improve the sterilization effect, the sterilization effect of the disinfection and sterilization wet wipe is insufficient; Chinese Patent Application No. CN202410896513.9 discloses a long-acting disinfection and sterilization wet (towel) paper, which uses functional small molecules or macromolecules or / and transition coupling agents or functional polymer materials as the functional main body, and macromolecules or polymer materials as the structural main body, and is prepared through processing technologies such as spraying, dip coating, soaking, bonding, and blending. However, functional small molecules or macromolecules or / and transition coupling agents are organic chemical components, and some components have relatively large irritation to the human skin, and their use is restricted; therefore, it is necessary to develop a wet wipe with excellent bactericidal performance and low irritation to meet the market demand. Summary of the Invention

[0004] In view of the deficiencies of the prior art, the present invention provides a germicidal wet wipe and its manufacturing process. The germicidal wet wipe has a germicidal rate of over 98% against Staphylococcus aureus, Escherichia coli, Candida albicans, and Bacillus subtilis within a short period of time, excellent germicidal performance, good moisturizing effect, no skin irritation, and high safety.

[0005] The technical solution adopted by the present invention is as follows: A germicidal wet wipe, which includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution includes the following components in parts by weight: 10 - 15 parts of a germicidal component; 0.5 - 1 part of a plant essential oil; 90 - 100 parts of water; the germicidal component is composed of modified carboxymethyl chitosan and modified sodium hyaluronate with a mass ratio of 1 - 2:1.

[0006] Further, the plant essential oil is any one or a mixture of several of rose essential oil, lavender essential oil, chamomile essential oil, and tea tree essential oil.

[0007] In the technical solution of the present invention, the preparation method of the modified carboxymethyl chitosan is: adding a 50 - 60wt% ethanol solution and carboxymethyl chitosan into a reaction vessel, stirring evenly, adding a modifying compound and triethylamine, and stirring and reacting at a temperature of 50 - 60°C for 5 - 6h to obtain the modified carboxymethyl chitosan.

[0008] Further, the mass ratio of the carboxymethyl chitosan, modifying compound, triethylamine, and 50 - 60wt% ethanol solution is 1:0.4 - 0.5:0.08 - 0.1:25 - 30.

[0009] In the technical solution of the present invention, the preparation method of the modified sodium hyaluronate is: adding water, sodium hyaluronate, and sodium hydroxide into a reaction vessel, stirring evenly, adding a modifying compound, and stirring and reacting at a temperature of 60 - 70°C for 8 - 10h to obtain the modified sodium hyaluronate.

[0010] Further, the mass ratio of the sodium hyaluronate, modifying compound, sodium hydroxide, and water is 1:0.5 - 0.6:0.1 - 0.15:15 - 20.

[0011] In the technical solution of the present invention, the preparation method of the modifying compound in the preparation methods of the modified carboxymethyl chitosan and the modified sodium hyaluronate is as follows: S1. Adding ethanol, 4 - chloroaniline, and dodecyl dimethyl tertiary amine into a reaction vessel, and refluxing and reacting at a temperature of 80°C for 10 - 11h to obtain intermediate 1; S2. Adding chloroform and intermediate 1 into a reaction vessel, slowly dropping epichlorohydrin, and after dropping, stirring and reacting at a temperature of 30 - 40°C for 3 - 5h to obtain intermediate 2; S3. Add toluene, intermediate 2, and guanidinoacetic acid into a reaction vessel, dropwise add concentrated sulfuric acid. After dropping, under the condition of a temperature of 110 °C, reflux and react for 6 - 8 h to obtain a modified compound, and the modified compound is: .

[0012] Further, the molar ratio of 4-chloroaniline to dodecyldimethyl tertiary amine in step S1 is 1:1.1 - 1.2.

[0013] Further, the molar ratio of intermediate 1 to epichlorohydrin in step S2 is 1:1 - 1.1.

[0014] Further, the molar ratio of intermediate 2, guanidinoacetic acid, and concentrated sulfuric acid in step S3 is 1:1.1 - 1.2:0.05 - 0.1.

[0015] The present invention also provides a manufacturing process for a bactericidal wet wipe, including the following steps: A1. Preparation of the wet wipe soaking solution: According to the weight ratio, add the bactericidal components of modified carboxymethyl chitosan and modified sodium hyaluronate, and plant essential oil into water, and stir evenly to obtain the wet wipe soaking solution; A2. Preparation of the bactericidal wet wipe: Spray the wet wipe soaking solution onto a wet wipe substrate to obtain the bactericidal wet wipe.

[0016] Further, the stirring speed in step A1 is 500 - 600 r / min, and the stirring time is 20 - 30 min.

[0017] The present invention has the following beneficial effects: In order to obtain a bactericidal wet wipe with excellent bactericidal performance, the present invention modifies the bactericidal components in the wet wipe soaking solution. First, using 4-chloroaniline as a raw material, it reacts with dodecyldimethyl tertiary amine, epichlorohydrin, and guanidinoacetic acid in sequence to prepare a modified compound. The modified compound contains a long-chain quaternary ammonium salt, guanidine group, and chloroalkyl group. The long-chain quaternary ammonium salt and guanidine group endow the modified compound with a dual bactericidal effect; on the one hand, the present invention modifies carboxymethyl chitosan which itself has a bactericidal effect, and uses the amino group in the molecular structure of carboxymethyl chitosan to react with the chloroalkyl group in the modified compound to graft the modified compound onto the carboxymethyl chitosan molecule, further enhancing the bactericidal effect of carboxymethyl chitosan; on the other hand, the present invention modifies sodium hyaluronate, and uses the hydroxyl group in the molecular structure of sodium hyaluronate to react with the chloroalkyl group in the modified compound to graft the modified compound onto the sodium hyaluronate molecule to obtain modified sodium hyaluronate with a bactericidal effect; the present invention uses the modified carboxymethyl chitosan and modified sodium hyaluronate in combination as the bactericidal components of the bactericidal wet wipe. Their synergistic effect can not only improve the moisturizing performance of the bactericidal wet wipe, but also greatly enhance the bactericidal performance of the bactericidal wet wipe.

[0018] The germicidal wet wipes provided by the present invention have a germicidal rate of over 98% against Staphylococcus aureus, Escherichia coli, Candida albicans and Bacillus subtilis within a short time, with excellent germicidal performance. At the same time, they also have good moisturizing effects, no skin irritation, high safety, and can better meet the market needs. Specific embodiments

[0019] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

[0020] In the technical solutions of the present invention, the raw materials used in the following embodiments and comparative examples are all ordinary commercially available products. Sodium hyaluronate, CAS No. 9067-32-7 (purchased from Beijing Wokai Biotechnology Co., Ltd., product number A45224, molecular weight 3000), carboxymethyl chitosan, CAS No. 83512-85-0 (molecular weight 8.5 kDa, deacetylation degree 90%, carboxymethylation degree 85%), sodium hydroxide, CAS No. 1310-73-2, triethylamine, CAS No. 121-44-8, 4-chloroaniline, CAS No. 106-47-8; dodecyldimethyl tertiary amine, CAS No. 112-18-5; epichlorohydrin, CAS No. 106-89-8, guanidinoacetic acid, CAS No. 352-97-6, concentrated sulfuric acid, CAS No. 7664-93-9, ethanol, CAS No. 64-17-5, chloroform, CAS No. 67-66-3, toluene, CAS No. 108-88-3, ether, CAS No. 60-29-7, acetone, CAS No. 67-64-1, are all analytical pure reagents.

[0021] Example 1 This example provides a preparation method of a modified compound, and the synthesis route is as follows: ; S1. Add 500 mL of ethanol, 13.0 g of 4-chloroaniline, and 23.9 g of dodecyldimethyl tertiary amine to a reaction vessel, and reflux for 11 h at a temperature of 80 °C. After the reaction is completed, cool, remove ethanol, add ether for stirring, filter, and dry the solid to obtain 29.5 g of intermediate 1; the molar ratio of 4-chloroaniline to dodecyldimethyl tertiary amine is 1:1.1; intermediate 1: ESI(m / z): 341.3[M+H] + , 1 H-NMR(600MHz, DMSO-d 6, δ ppm): 6.85 (d, J = 8.3 Hz, 2H), 6.80 (d, J = 8.3 Hz, 2H), 5.50 (s, 2H), 3.74 (s, 6H), 3.23 - 3.27 (m, 2H), 1.72 - 1.78 (m, 2H), 1.24 - 1.31 (m, 18H), 0.88 - 0.93 (m, 3H); S2. Add 400 mL of chloroform and 25.0 g of Intermediate 1 to a reaction vessel, slowly dropwise add 7.1 g of epichlorohydrin. After dropping, stir and react at a temperature of 35 °C for 5 h. After the reaction is completed, cool, remove the chloroform, add ether and stir, filter, and dry the solid to obtain 27.2 g of Intermediate 2; the molar ratio of Intermediate 1 to epichlorohydrin is 1:1.05; Intermediate 2: ESI(m / z): 433.3[M + H] + , 1 H-NMR(600 MHz, DMSO-d 6 , δ ppm): 9.53 (s, 1H), 6.90 (d, J = 8.3 Hz, 2H), 6.67 (d, J = 8.3 Hz, 2H), 5.37 (s, 1H), 3.64 - 3.76 (m, 8H), 3.32 - 3.39 (m, 2H), 3.18 - 3.25 (m, 3H), 1.71 - 1.76 (m, 2H), 1.25 - 1.31 (m, 18H), 0.88 - 0.92 (m, 3H); S3. Add 300 mL of toluene, 25.0 g of Intermediate 2, and 7.4 g of guanidinoacetic acid to a reaction vessel, dropwise add 0.3 g of concentrated sulfuric acid. After dropping, reflux and react at a temperature of 110 °C for 8 h. After the reaction is completed, cool, remove the toluene, add ether and stir, filter, and dry the solid to obtain 25.8 g of the modified compound; the molar ratio of Intermediate 2 to guanidinoacetic acid to concentrated sulfuric acid is 1:1.1:0.05; modified compound: ESI(m / z): 532.3[M + H] + , 1 H-NMR(600 MHz, DMSO-d 6 , δ ppm): 9.52 (s, 1H), 7.84 (s, 1H), 6.92 (d, J = 8.3 Hz, 2H), 6.68 (d, J = 8.3 Hz, 2H), 6.63 (s, 2H), 4.93 - 4.98 (m, 1H), 4.32 (s, 2H), 3.70 - 3.76 (m, 7H), 3.40 - 3.48 (m, 2H), 3.15 - 3.23 (m, 3H), 2.50 (s, 1H), 1.72 - 1.78 (m, 2H), 1.23 - 1.30 (m, 18H), 0.87 - 0.92 (m, 3H).

[0022] Example 2 This example provides a bactericidal wet wipe and its manufacturing process.

[0023] A bactericidal wet wipe, which includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution includes the following components in parts by weight: 15 parts of a bactericidal component; 0.8 part of plant essential oil; 100 parts of water; the bactericidal component is composed of modified carboxymethyl chitosan and modified sodium hyaluronate with a mass ratio of 2:1; the plant essential oil is rose essential oil.

[0024] The preparation method of modified carboxymethyl chitosan is as follows: Add 60wt% ethanol solution and carboxymethyl chitosan into a reaction vessel, stir evenly, add a modifying compound and triethylamine, stir and react at a temperature of 55°C for 6h. After the reaction is completed, cool, adjust the pH of the solution to neutral, drop the reaction solution into acetone for precipitation, filter, wash the solid with acetone, and dry to obtain modified carboxymethyl chitosan; the substitution degree of the modifying compound is determined to be 12.6% by elemental analysis; the mass ratio of carboxymethyl chitosan, modifying compound, triethylamine, and 60wt% ethanol solution is 1:0.5:0.1:30.

[0025] The preparation method of modified sodium hyaluronate is as follows: Add water, sodium hyaluronate, and sodium hydroxide into a reaction vessel, stir evenly, add a modifying compound, stir and react at a temperature of 70°C for 9h. After the reaction is completed, cool, drop the reaction solution into absolute ethanol for precipitation, filter, wash the solid with absolute ethanol, and dry to obtain modified sodium hyaluronate; the substitution degree of the structure of the modifying compound is determined to be 15.4% by nuclear magnetic resonance hydrogen spectrum; the mass ratio of sodium hyaluronate, modifying compound, sodium hydroxide, and water is 1:0.6:0.15:20.

[0026] A manufacturing process of a bactericidal wet wipe includes the following steps: A1. Preparation of the wet wipe soaking solution: According to the weight ratio, add the bactericidal component composed of modified carboxymethyl chitosan and modified sodium hyaluronate and plant essential oil into water, stir evenly to obtain the wet wipe soaking solution; the stirring speed is 600r / min, and the stirring time is 20min; A2. Preparation of the bactericidal wet wipe: Spray the wet wipe soaking solution on the wet wipe substrate to obtain the bactericidal wet wipe.

[0027] Example 3 This example provides a bactericidal wet wipe and its manufacturing process.

[0028] A bactericidal wet wipe, which includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution includes the following components in parts by weight: 13 parts of a bactericidal component; 1 part of plant essential oil; 95 parts of water; the bactericidal component is composed of modified carboxymethyl chitosan and modified sodium hyaluronate with a mass ratio of 1.5:1; the plant essential oil is tea tree essential oil.

[0029] The preparation method of modified carboxymethyl chitosan is as follows: Add 55wt% ethanol solution and carboxymethyl chitosan into a reaction vessel, stir evenly, add a modifying compound and triethylamine, stir and react at a temperature of 60°C for 5h. After the reaction is completed, cool, adjust the pH of the solution to neutral, drop the reaction solution into acetone for precipitation, filter, wash the solid with acetone, and dry to obtain modified carboxymethyl chitosan; the substitution degree of the modifying compound is determined to be 12.0% by elemental analysis; the mass ratio of carboxymethyl chitosan, modifying compound, triethylamine, and 55wt% ethanol solution is 1:0.45:0.09:25.

[0030] The preparation method of modified sodium hyaluronate is as follows: Add water, sodium hyaluronate, and sodium hydroxide into a reaction vessel, stir evenly, add a modifying compound, stir and react at a temperature of 60°C for 10h. After the reaction is completed, cool, drop the reaction solution into absolute ethanol for precipitation, filter, wash the solid with absolute ethanol, and dry to obtain modified sodium hyaluronate; the substitution degree of the structure of the modifying compound is determined to be 14.2% by nuclear magnetic resonance hydrogen spectrum; the mass ratio of sodium hyaluronate, modifying compound, sodium hydroxide, and water is 1:0.5:0.1:15.

[0031] A manufacturing process of a germicidal wet wipe includes the following steps: A1. Preparation of the wet wipe soaking solution: According to the weight ratio, add the germicidal components of modified carboxymethyl chitosan and modified sodium hyaluronate and plant essential oil into water, stir evenly to obtain the wet wipe soaking solution; the stirring speed is 500r / min, and the stirring time is 30min; A2. Preparation of the germicidal wet wipe: Spray the wet wipe soaking solution onto the wet wipe substrate to obtain the germicidal wet wipe.

[0032] Example 4 This example provides a germicidal wet wipe and its manufacturing process.

[0033] A germicidal wet wipe includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution includes the following components in parts by weight: 10 parts of germicidal components; 0.5 part of plant essential oil; 90 parts of water; the germicidal components are composed of modified carboxymethyl chitosan and modified sodium hyaluronate with a mass ratio of 1:1; the plant essential oil is chamomile essential oil.

[0034] The preparation method of modified carboxymethyl chitosan is as follows: Add 50wt% ethanol solution and carboxymethyl chitosan into a reaction vessel, stir evenly, add a modified compound and triethylamine, and stir and react at a temperature of 50°C for 5.5 h. After the reaction is completed, cool it, adjust the pH of the solution to neutral, drop the reaction solution into acetone for precipitation, filter, wash the solid with acetone, and dry it to obtain modified carboxymethyl chitosan; the substitution degree of the modified compound is determined to be 11.3% by elemental analysis; the mass ratio of carboxymethyl chitosan, modified compound, triethylamine, and 50wt% ethanol solution is 1:0.4:0.08:28.

[0035] The preparation method of modified sodium hyaluronate is as follows: Add water, sodium hyaluronate, and sodium hydroxide into a reaction vessel, stir evenly, add a modified compound, and stir and react at a temperature of 65°C for 8 h. After the reaction is completed, cool it, drop the reaction solution into absolute ethanol for precipitation, filter, wash the solid with absolute ethanol, and dry it to obtain modified sodium hyaluronate; the substitution degree of the structure of the modified compound is determined to be 14.8% by nuclear magnetic resonance hydrogen spectrum; the mass ratio of sodium hyaluronate, modified compound, sodium hydroxide, and water is 1:0.55:0.13:18.

[0036] A manufacturing process of a bactericidal wet wipe includes the following steps: A1. Preparation of the wet wipe soaking solution: According to the weight ratio, add the bactericidal components of modified carboxymethyl chitosan and modified sodium hyaluronate and plant essential oil into water, stir evenly to obtain the wet wipe soaking solution; the stirring speed is 550 r / min, and the stirring time is 25 min; A2. Preparation of the bactericidal wet wipe: Spray the wet wipe soaking solution onto the wet wipe substrate to obtain the bactericidal wet wipe.

[0037] Example Five This example provides a bactericidal wet wipe and its manufacturing process.

[0038] A bactericidal wet wipe includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution includes the following components in parts by weight: 14 parts of bactericidal components; 0.7 part of plant essential oil; 97 parts of water; the bactericidal components are composed of modified carboxymethyl chitosan and modified sodium hyaluronate with a mass ratio of 1.8:1; the plant essential oil is lavender essential oil.

[0039] The preparation method of modified carboxymethyl chitosan is as follows: Add 60wt% ethanol solution and carboxymethyl chitosan into a reaction vessel, stir evenly, add a modified compound and triethylamine, and stir and react at 60°C for 6 hours. After the reaction is completed, cool it, adjust the pH of the solution to neutral, drop the reaction solution into acetone for precipitation, filter, wash the solid with acetone, and dry it to obtain modified carboxymethyl chitosan; the substitution degree of the modified compound is determined to be 12.4% by elemental analysis; the mass ratio of carboxymethyl chitosan, modified compound, triethylamine, and 60wt% ethanol solution is 1:0.5:0.08:30.

[0040] The preparation method of modified sodium hyaluronate is as follows: Add water, sodium hyaluronate, and sodium hydroxide into a reaction vessel, stir evenly, add a modified compound, and stir and react at 70°C for 8 hours. After the reaction is completed, cool it, drop the reaction solution into absolute ethanol for precipitation, filter, wash the solid with absolute ethanol, and dry it to obtain modified sodium hyaluronate; the substitution degree of the structure of the modified compound is determined to be 15.1% by nuclear magnetic resonance hydrogen spectrum; the mass ratio of sodium hyaluronate, modified compound, sodium hydroxide, and water is 1:0.6:0.1:20.

[0041] A manufacturing process for a germicidal wet wipe includes the following steps: A1. Preparation of the wet wipe soaking solution: According to the weight ratio, add the germicidal components of modified carboxymethyl chitosan and modified sodium hyaluronate and plant essential oil into water, stir evenly to obtain the wet wipe soaking solution; the stirring speed is 580 r / min, and the stirring time is 24 minutes. A2. Preparation of the germicidal wet wipe: Spray the wet wipe soaking solution onto the wet wipe substrate to obtain the germicidal wet wipe.

[0042] Comparative Example 1 This comparative example provides a germicidal wet wipe and its manufacturing process.

[0043] A germicidal wet wipe includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution includes the following components in parts by weight: 15 parts of germicidal components; 0.8 part of plant essential oil; 100 parts of water; the germicidal components are composed of carboxymethyl chitosan and modified sodium hyaluronate with a mass ratio of 2:1; the plant essential oil is rose essential oil.

[0044] The preparation method of modified sodium hyaluronate is the same as that in Example 2.

[0045] A manufacturing process for a germicidal wet wipe is the same as that in Example 2.

[0046] Comparative Example 2 This example provides a germicidal wet wipe and its manufacturing process.

[0047] A bactericidal wet wipe, which includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution includes the following components in parts by weight: 15 parts of a bactericidal component; 0.8 part of plant essential oil; 100 parts of water; the bactericidal component is composed of modified carboxymethyl chitosan and sodium hyaluronate with a mass ratio of 2:1; the plant essential oil is rose essential oil.

[0048] The preparation method of modified carboxymethyl chitosan is the same as that in Example 2.

[0049] The manufacturing process of a bactericidal wet wipe is the same as that in Example 2.

[0050] Comparative Example 3 This example provides a bactericidal wet wipe and its manufacturing process.

[0051] A bactericidal wet wipe, which includes a wet wipe substrate and a wet wipe soaking solution; the wet wipe soaking solution includes the following components in parts by weight: 15 parts of a bactericidal component; 0.8 part of plant essential oil; 100 parts of water; the bactericidal component is composed of carboxymethyl chitosan and sodium hyaluronate with a mass ratio of 2:1; the plant essential oil is rose essential oil.

[0052] The manufacturing process of a bactericidal wet wipe is the same as that in Example 2.

[0053] 1. Bactericidal performance test of bactericidal wet wipes The bactericidal performance of the bactericidal wet wipes prepared in Examples 2 to 5 and Comparative Examples 1 to 3 was tested. Referring to Appendix C of the Hygiene Standard for Disposable Sanitary Products GB / T15979-2002, the test bacteria were Staphylococcus aureus (ATCC6538), Escherichia coli (ATCC 25922), Candida albicans (ATCC 14053), and Bacillus subtilis (ATCC 6633), and the contact time was 2 min; the results are shown in Table 1 below.

[0054] Table 1 Bactericidal test results As can be seen from the results in Table 1, the bactericidal rates of the bactericidal wet wipes prepared in Examples 2 to 4 of the present invention against Staphylococcus aureus, Escherichia coli, Candida albicans, and Bacillus subtilis all reached over 98% in a short time, indicating that the bactericidal wet wipes provided by the present invention have excellent bactericidal performance; compared with Comparative Examples 1 to 3, the bactericidal components in the bactericidal wet wipe of Example 2 of the present invention are modified carboxymethyl chitosan and modified sodium hyaluronate, both of which are grafted with modified compound structures containing bactericidal long-chain quaternary ammonium salts and guanidine groups, and the two act synergistically to exert multiple bactericidal effects, thereby greatly enhancing the bactericidal performance of the bactericidal wet wipe.

[0055] 2. Skin irritation and moisturizing performance test of bactericidal wet wipes 35 volunteers were randomly selected (aged 25 - 30 years old, in good health, and with no wounds on the skin of the right hand back). The bactericidal wet wipes prepared in Examples 2 - 5 and Comparative Examples 1 - 3 were used once in the morning and once in the evening every day to wipe the skin of the right hand back. They were used continuously for one week, and no moisturizing products were used during this period. After the use was completed, the skin irritation of the bactericidal wet wipes was evaluated by observing whether allergic reactions such as erythema, papules, edema, itching, pain, and burning sensation occurred on the skin of the right hand back; in addition, the water content of the epidermis of the skin of the right hand back was measured with a skin moisture pen, and the measurement was taken three times at the same location each time, and the average value was taken. The moisturizing performance of the bactericidal wet wipes was evaluated through the water content values of the skin epidermis before and after use; the results are shown in Table 2 below.

[0056] Table 2 Test Results of Skin Irritation and Moisturizing Performance As can be seen from the results in Table 2, the bactericidal wet wipes prepared in Examples 2 - 4 of the present invention have no skin irritation and have good moisturizing effects; the bactericidal components in the bactericidal wet wipes prepared in the present invention are modified carboxymethyl chitosan and modified sodium hyaluronate, and the two can synergistically enhance the moisturizing performance of the bactericidal wet wipes and increase the water content of the skin epidermis.

[0057] It should be noted that in this article, the term "including", "comprising" or any other variant thereof is intended to cover non - exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements but also includes other elements not explicitly listed, or further includes elements inherent to such a process, method, article or device; although the embodiments of the present application have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A sterilizing wet wipe, characterized in that: The bactericidal wet wipes include a wet wipe base material and a wet wipe soaking liquid; the wet wipe soaking liquid includes the following components by weight: 10-15 parts of bactericidal ingredients; 0.5-1 parts of plant essential oils; 90-100 parts of water; the bactericidal ingredients are composed of modified carboxymethyl chitosan and modified sodium hyaluronate in a mass ratio of 1-2:1; The preparation method of the modified carboxymethyl chitosan is as follows: 50-60wt% ethanol solution and carboxymethyl chitosan are added into a reaction container, stirred evenly, and a modified compound and triethylamine are added, and stirred and reacted for 5-6h at a temperature of 50-60°C to obtain the modified carboxymethyl chitosan; The preparation method of the modified sodium hyaluronate is as follows: water, sodium hyaluronate and sodium hydroxide are added into a reaction container, stirred evenly, and a modified compound is added, and stirred and reacted at a temperature of 60-70° C. for 8-10 hours to obtain the modified sodium hyaluronate.

2. A sterilizing wet wipe according to claim 1, characterized in that: The preparation methods of the modified compounds in the preparation methods of modified carboxymethyl chitosan and modified sodium hyaluronate are: S1, adding ethanol, 4-chloroaniline and dodecyldimethylamine into a reaction vessel, and refluxing for 10-11 hours at 80°C to obtain intermediate 1; S2, adding chloroform and intermediate 1 into a reaction vessel, slowly adding epichlorohydrin dropwise, stirring and reacting at a temperature of 30-40° C. for 3-5 hours to obtain intermediate 2; S3. Add toluene, intermediate 2 and guanidine acetic acid into a reaction container, add concentrated sulfuric acid dropwise, and after the addition is complete, reflux the reaction at 110° C. for 6-8 hours to obtain a modified compound.

3. A bactericidal wet wipe according to claim 2, characterized in that: The molar ratio of 4-chloroaniline to dodecyldimethyl tertiary amine in step S1 is 1:1.1-1.

2.

4. A bactericidal wet wipe according to claim 2, characterized in that: The molar ratio of the intermediate 1 to epichlorohydrin in step S2 is 1:1-1.

1.

5. A sterilizing wet wipe according to claim 2, characterized in that: The molar ratio of the intermediate 2, guanidinoacetic acid and concentrated sulfuric acid in step S3 is 1:1.1-1.2:0.05-0.

1.

6. A sterilizing wet wipe according to claim 1, characterized in that: In the preparation method of modified carboxymethyl chitosan, the mass ratio of the carboxymethyl chitosan, the modified compound, triethylamine and 50-60wt% ethanol solution is 1:0.4-0.5:0.08-0.1:25-30.

7. A bactericidal wet wipe according to claim 1, characterized in that: In the preparation method of modified sodium hyaluronate, the mass ratio of sodium hyaluronate, modified compound, sodium hydroxide and water is 1:0.5-0.6:0.1-0.15:15-20.

8. A bactericidal wet wipe according to claim 1, characterized in that: The plant essential oil is any one of rose essential oil, lavender essential oil, chamomile essential oil and tea tree essential oil or a mixture of several of them.

9. A process for producing a bactericidal wet wipe according to any one of claims 1 to 8, characterized in that: The steps include: A1. Preparation of wet wipes soaking liquid: adding bactericidal components of modified carboxymethyl chitosan and modified sodium hyaluronate and plant essential oil to water according to weight ratio, stirring evenly to obtain wet wipes soaking liquid; A2. Preparation of bactericidal wet wipes: spray the wet wipe soaking liquid onto the wet wipe substrate to obtain the bactericidal wet wipes.

10. The manufacturing process of a bactericidal wet wipe according to claim 9, characterized in that: In step A1, the stirring speed is 500-600 r / min, and the stirring time is 20-30 min.

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