Hypoglycemic composition as well as preparation method and application thereof

Abstract

The invention provides a hypoglycemic composition as well as a preparation method and application thereof, and belongs to the field of medicines. The blood sugar reducing composition is prepared from 20-30 parts of jerusalem artichoke, 10-20 parts of alkaline land dandelion and 5-15 parts of abelmoschus manihot, and the invention further provides a preparation method and application of the blood sugar reducing composition. The composition for reducing blood sugar is simple in raw material composition and low in cost, and the jerusalem artichoke, the alkaline land dandelion and the abelmoschus manihot are matched with one another in a specific proportion and act together, so that the composition has a remarkable effect of reducing blood sugar.

Description

Technical Field

[0001] The invention belongs to the field of medicine, and specifically relates to a blood sugar lowering composition and a preparation method and application thereof. Background Art

[0002] Diabetes is an endocrine metabolic disease caused by absolute or relative insulin deficiency and utilization disorder, characterized by hyperglycemia and protein and fat metabolism disorders as the main clinical manifestations. The disease is mainly divided into three types: type 1 diabetes, type 2 diabetes and gestational diabetes. Type 2 diabetes mellitus (T2DM) is the most common type of diabetes. It is caused by a variety of causes that lead to insufficient insulin secretion or the body's inability to effectively utilize insulin, resulting in a continuous increase in blood sugar levels, often accompanied by various complications such as heart disease, liver disease, and kidney disease. At present, most clinical treatments for diabetes use blood sugar-lowering Western medicine or artificial insulin injections. Although the blood sugar-lowering effect is obvious, the toxic side effects are large. Not only is it easy to develop drug resistance in the long run, but it also increases the risk of cardiovascular and cerebrovascular diseases. While affecting the quality of life of patients, it also imposes a heavy economic burden on families and society.

[0003] Studies have found that the functional components of many medicinal and edible plants have good blood sugar lowering effects and long-term use will not cause damage to the body. Therefore, obtaining effective and safe blood sugar lowering compositions from natural functional plants is of great significance for the prevention and treatment of diabetes and its complications. Summary of the invention

[0004] In order to solve the problems existing in the prior art, the purpose of the present invention is to provide a hypoglycemic composition, which is based on the mutual cooperation and synergistic effect of three medicinal and edible plants, Jerusalem artichoke, alkali dandelion and golden sunflower, to achieve a significant hypoglycemic effect.

[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0006] The invention provides a hypoglycemic composition, which is prepared from the following raw materials by weight: 20-30 parts of Jerusalem artichoke, 10-20 parts of alkali soil dandelion and 5-15 parts of golden sunflower.

[0007] The present invention also provides a method for preparing the above-mentioned hypoglycemic composition, comprising the following steps: mixing raw material powder with ethanol to obtain a mixed system; adding pectinase and cellulase to the mixed system, ultrasonic extraction, centrifuging and filtering the extract to obtain filter residue and filtrate A; hot water extraction of the filter residue, centrifuging and filtering the extract to obtain filtrate B; combining filtrate A and filtrate B, concentrating under reduced pressure, freeze-drying in vacuum, and pulverizing to obtain the hypoglycemic composition.

[0008] Preferably, the material-liquid ratio of the raw material powder to ethanol is 1 g:10-30 mL; and the ethanol is 60%-80% ethanol.

[0009] Preferably, based on the mixed system, the added amount of the pectinase and the cellulase is 1-5g:100mL; the mass ratio of the pectinase and the cellulase is 1-3:1; the enzyme activity of the pectinase is 25000-35000U / g, and the enzyme activity of the cellulase is 5000-15000U / g.

[0010] Preferably, the ultrasonic extraction has a power of 500-700 W, a temperature of 50-70° C., and a time of 0.5-1.5 h.

[0011] Preferably, during the hot water extraction, the solid-liquid ratio of the filter residue to water is 1 g:5-15 mL.

[0012] Preferably, the hot water extraction temperature is 70-90° C. and the time is 1-2 h.

[0013] Preferably, the centrifugal conditions are: 7000-9000 r / min, 10-20 min, 18-25°C.

[0014] Preferably, the conditions for the reduced pressure concentration are: -0.06 to -0.10 MPa, 50 to 60° C.; the reduced pressure concentration is performed to a relative density of 1.25 to 1.35.

[0015] The present invention also provides the use of the above-mentioned hypoglycemic composition or the above-mentioned preparation method in preparing a drug for preventing and / or treating diabetes.

[0016] Compared with the prior art, the technical solution of the present invention has the following beneficial effects:

[0017] The present invention provides a hypoglycemic composition with simple composition, good safety, high efficiency and low cost based on three medicinal and edible plants, namely Jerusalem artichoke, dandelion and sunflower. The optimal extraction scheme is obtained through experimental compounding to obtain a higher yield of effective ingredients, and the hypoglycemic activity of the composition is tested. Compared with a single extract or an extract of a combination of two components, the effects are complementary and the synergistic hypoglycemic effect is better. DETAILED DESCRIPTION

[0018] The present invention provides a hypoglycemic composition, which is made of the following raw materials by weight: 20-30 parts of Jerusalem artichoke, 10-20 parts of dandelion and 5-15 parts of golden sunflower. The hypoglycemic composition is preferably made of 22-25 parts of Jerusalem artichoke, 15-18 parts of dandelion and 10-12 parts of golden sunflower. The present invention is based on three medicinal and edible plants, namely Jerusalem artichoke, dandelion and golden sunflower, which cooperate with each other and work together in a specific ratio to achieve a significant synergistic hypoglycemic effect.

[0019] Jerusalem artichoke (Helianthus tuberosus L.), also known as sunroot, is a perennial herbaceous plant belonging to the genus Helianthus of the Compositae family. It is usually used as medicine with its tuberous roots, stems and leaves. It tastes sweet; slightly bitter; cool in nature. Its functions and indications are: clearing heat and cooling blood, and setting bones. It is mainly used to treat fevers, bloody diarrhea due to intestinal heat, and traumatic injuries. In the present invention, the part of Jerusalem artichoke used is the tuberous root.

[0020] Taraxacum borealisinense Kitam. mainly grows in specific environments such as slightly moist saline-alkali land. There are blackish-brown remaining leaf bases at the root neck. The leaves are narrowly oblanceolate or oblanceolate-elliptic, 4 - 12 cm long and 0.5 - 2 cm wide, and sometimes have undulate teeth or pinnately divided lobes at the edges. Due to the difference in the growth environment from other dandelions, there are differences in the specific proportions and types of active ingredients, resulting in differences in the emphasis of pharmacological effects. In the present invention, the part of Taraxacum borealisinense Kitam. used is the whole plant.

[0021] Aurea helianthus., also known as Hibiscus trionum var. aurantiacus, is an annual herbaceous plant belonging to the genus Abelmoschus of the Malvaceae family. It is recorded in "Compendium of Materia Medica" that it has the effects of clearing away damp-heat and relieving inflammation and pain. Orally, it is mainly used to treat stranguria, edema, and externally for burns caused by hot water. In the present invention, the part of Aurea helianthus. used is the flower.

[0022] The present invention also provides a preparation method of a hypoglycemic composition, which includes the following steps: mixing raw material powder with ethanol to obtain a mixed system; adding pectinase and cellulase to the mixed system, and performing ultrasonic extraction. The extract is centrifuged and filtered to obtain filter residue and filtrate A; the filter residue is subjected to hot water extraction, and the extraction solution is centrifuged and filtered to obtain filtrate B; filtrate A and filtrate B are combined, and after concentration under reduced pressure, vacuum freeze-drying and pulverization, a hypoglycemic composition is obtained.

[0023] The raw material powder in the present invention is obtained by washing, drying and pulverizing Jerusalem artichoke, Taraxacum borealisinense Kitam. and Aurea helianthus.. The pulverization can be a conventional pulverization method, and after pulverization, it is sieved through a 50 - 80 mesh sieve, preferably through a 60 - 70 mesh sieve. As an alternative implementation manner, the present invention pulverizes each raw material separately and then mixes them to obtain the raw material powder; as another alternative implementation manner, the present invention mixes each raw material and pulverizes them simultaneously to obtain the raw material powder.

[0024] The present invention mixes the raw material powder with ethanol, and the material-liquid ratio of the raw material powder to ethanol is 1 g:10 - 30 mL, preferably 1 g:15 - 25 mL, more preferably 1 g:20 mL; the ethanol is 60% - 80% ethanol (the volume ratio of ethanol in the solution is 60% - 80%), preferably 65% - 75% ethanol, more preferably 70% ethanol.

[0025] In the mixed system of raw material powder and ethanol of the present invention, pectinase and cellulase are added. Based on the mixed system, the (total) addition amount of the pectinase and cellulase is 1-5 g:100 mL, preferably 2-4 g:100 mL, more preferably 3 g:100 mL; the mass ratio of the pectinase and cellulase is 1-3:1, preferably 2:1; the enzyme activity of the pectinase is 25000-35000 U / g, preferably 30000 U / g; the enzyme activity of the cellulase is 5000-15000 U / g, preferably 10000 U / g. After adding the pectinase and cellulase in the present invention, it is preferred to maintain the pH of the system at 3.8-4.2, more preferably 4.0. As an alternative implementation, sodium hydroxide or hydrochloric acid is used in the present invention to adjust the pH of the system.

[0026] After adding the pectinase and cellulase in the present invention, ultrasonic extraction is carried out. The power of the ultrasonic extraction is 500-700 W, preferably 600 W; the temperature of the ultrasonic extraction is 50-70 °C, preferably 55-65 °C, more preferably 60 °C; the time of the ultrasonic extraction is 0.5-1.5 h, preferably 1 h.

[0027] The present invention preferably repeats the ultrasonic extraction 2 times, and the method of the second extraction is the same as that of the first time. That is, after the first ultrasonic extraction, the filtrate is collected, the filter residue is mixed with ethanol again, pectinase and cellulase are added, and ultrasonic extraction is carried out, wherein the material-liquid ratio of the filter residue to ethanol, the ethanol concentration, the addition amount of pectinase and cellulase, and the ultrasonic extraction conditions are the same as those of the first time.

[0028] The present invention centrifuges and filters the extract obtained by ultrasonic extraction (the filtrate obtained when ultrasonic extraction is carried out once or the filtrate obtained by combining the filtrates of two ultrasonic extractions) to obtain a filter residue and filtrate A. The conditions of the centrifugation are: 7000-9000 r / min, 10-20 min, 18-25 °C; the centrifugation speed is preferably 8000 r / min; the centrifugation time is preferably 15 min; the centrifugation temperature is preferably 20 °C.

[0029] The present invention carries out hot water extraction on the filter residue after ultrasonic extraction. During the hot water extraction, the material-liquid ratio of the filter residue to water is 1 g:5-15 mL, preferably 1 g:8-12 mL, more preferably 1 g:10 mL. The temperature of the hot water extraction is 70-90 °C, preferably 75-85 °C, more preferably 80 °C; the time of the hot water extraction is 1-2 h, preferably 1.5 h.

[0030] The present invention preferably repeats the hot water extraction 2 times, and the method of the second extraction is the same as that of the first time. That is, after the first hot water extraction, the filtrate is collected, the filter residue is extracted with water again, and the material-liquid ratio of the filter residue to water and the extraction conditions are the same as those of the first time.

[0031] The leaching solution obtained by hot water extraction (the filtrate obtained by hot water extraction once or the filtrate obtained by combining the filtrates of two hot water extractions) of the present invention is centrifuged and filtered to obtain filtrate B. The conditions for centrifugation are: 7000-9000 r / min, 10-20 min, 18-25 °C; the centrifugation speed is preferably 8000 r / min; the centrifugation time is preferably 15 min; the centrifugation temperature is preferably 20 °C.

[0032] The present invention combines filtrate A and filtrate B, and after concentration under reduced pressure, vacuum freeze-drying, and pulverization, a hypoglycemic composition is obtained. The conditions for concentration under reduced pressure are: -0.06 to -0.10 Mpa, 50-60 °C; the temperature for concentration under reduced pressure is preferably 55 °C; the concentration under reduced pressure is to a relative density of 1.25-1.35, preferably 1.30. The vacuum freeze-drying can be: pre-freezing at -80 °C for 24 h, and after pre-freezing, drying is carried out at a temperature of -40 °C to 40 °C under a vacuum degree of 8-12 Pa. The drying process is to dry for 2 h for every 10 °C increase, and the total drying time is 18 h. The pulverization can be a conventional pulverization method, and after pulverization, it is sieved through a 50-80 mesh sieve, preferably through a 60-70 mesh sieve.

[0033] The present invention also provides the use of the hypoglycemic composition or its preparation method in the preparation of a drug for preventing and / or treating diabetes, and the diabetes is preferably type 2 diabetes. When preparing a drug for preventing and / or treating diabetes according to the present invention, the hypoglycemic composition described in the present invention is used as the main medicinal ingredient, and pharmaceutically acceptable excipients are added to prepare a medicament, and the medicament includes but is not limited to tablets, pills, and powders.

[0034] Next, the technical solutions in the present invention will be clearly and completely described in conjunction with the embodiments in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0035] In the specific embodiments of the present invention, Jerusalem artichoke, Taraxacum borealisinense Kitag., and Abelmoschus manihot are all obtained through regular commercial channels. The origin of Taraxacum borealisinense Kitag. is Tangshan City, Hebei Province; the other dandelion is Taraxacum mongolicum Hand.-Mazz., and the origin is Anguo City, Baoding City, Hebei Province.

[0036] In the following embodiments, unless otherwise specified, they are all conventional methods.

[0037] The materials, reagents, etc. used in the following embodiments can all be obtained from commercial channels unless otherwise specified.

[0038] Example 1

[0039] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke, 10 parts of Taraxacum sinicum Kitag. and 5 parts of Abelmoschus manihot (L.) Medic. by mass.

[0040] The preparation method is as follows:

[0041] (1) Weigh each raw material in the composition according to the proportion, wash, dry, pulverize and pass through a 60-mesh sieve to obtain a mixed powder S1.

[0042] (2) Add 70% ethanol to S1 according to the solid-liquid ratio of 1 g:20 mL, add pectinase and cellulase according to the solid-liquid ratio of 2 g:100 mL (the mass ratio of pectinase and cellulase is 2:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), and perform ultrasonic extraction (power 600 W) at 60 °C for 1 h, filter, and collect the filtrate; repeat the above steps for the filter residue (the parameter settings are exactly the same) to obtain a filtrate. Combine the filtrates extracted twice, centrifuge (8000 r / min, 15 min, 20 °C) and filter after cooling, collect the supernatant to obtain filtrate A and filter residue.

[0043] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction according to the solid-liquid ratio of 1 g:10 mL, keep the hot water temperature at 70 °C, extract for 1.5 h, filter, and collect the filtrate; repeat the above steps for the filter residue (the parameter settings are exactly the same) to obtain a filtrate. Combine the filtrates extracted twice, centrifuge (8000 r / min, 15 min, 20 °C) and filter after cooling, collect the supernatant to obtain filtrate B.

[0044] (4) Combine filtrate A and filtrate B, keep the pressure at -0.08 Mpa, and concentrate under reduced pressure at 55 °C to a relative density of 1.25 and without alcohol smell, and perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, under a vacuum of 10 Pa, dry at a temperature of -40 °C to 40 °C, and the drying process is 2 h for every 10 °C rise, and the total drying time is 18 h) to obtain a mixed extract S2, pulverize and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0045] Example 2

[0046] A hypoglycemic composition is made of 25 parts of Jerusalem artichoke, 15 parts of Taraxacum sinicum Kitag. and 10 parts of Abelmoschus manihot (L.) Medic. by mass.

[0047] The preparation method is as follows:

[0048] (1) Weigh each raw material in the composition according to the proportion, wash, dry, pulverize and pass through a 60-mesh sieve to obtain a mixed powder S1.

[0049] (2) Add 70% ethanol to S1 at a solid-liquid ratio of 1 g:30 mL, and add pectinase and cellulase at a solid-liquid ratio of 1 g:100 mL (the mass ratio of pectinase to cellulase is 1:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4). Ultrasonically extract (power 600 W) at 50 °C for 1 h, filter, collect the filtrate, and repeat the above steps for the filter residue (with the same parameter settings) to obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (8000 r / min, 15 min, 20 °C), filter, collect the supernatant to obtain filtrate A and the filter residue.

[0050] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at a solid-liquid ratio of 1 g:5 mL, keep the hot water temperature at 70 °C, extract for 1.5 h, filter, collect the filtrate, and repeat the above steps for the filter residue (with the same parameter settings) to obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (8000 r / min, 15 min, 20 °C), filter, collect the supernatant to obtain filtrate B.

[0051] (4) Combine filtrate A and filtrate B, keep the pressure at -0.06 Mpa, and concentrate under reduced pressure at 55 °C to a relative density of 1.35 and without alcohol smell, then conduct vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, conduct drying at a vacuum degree of 8 Pa and a temperature range of -40 °C to 40 °C, the drying process is to dry for 2 h for every 10 °C increase, and the total drying time is 18 h) to obtain the mixed extract S2, pulverize and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0052] Example 3

[0053] A hypoglycemic composition is made of 30 parts of Jerusalem artichoke, 20 parts of Taraxacum sinicum Kitag. and 15 parts of Abelmoschus manihot (L.) Medic. by mass.

[0054] The preparation method is as follows:

[0055] (1) Weigh each raw material in the composition according to the proportion, wash, dry, pulverize and pass through a 60-mesh sieve to obtain the mixed powder S1.

[0056] (2) Add 80% ethanol to S1 at a solid-liquid ratio of 1 g:10 mL, and add pectinase and cellulase at a solid-liquid ratio of 1 g:100 mL (the mass ratio of pectinase to cellulase is 3:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4). Ultrasonically extract (power 600 W) at 60 °C for 1 h, filter, collect the filtrate, and repeat the above steps for the filter residue (with the same parameter settings) to obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (8000 r / min, 15 min, 20 °C), filter, collect the supernatant to obtain filtrate A and the filter residue.

[0057] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at a material-liquid ratio of 1 g: 15 mL, keep the hot water temperature at 70 °C, extract for 1.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (8000 r / min, 15 min, 20 °C), filter, collect the supernatant to obtain filtrate B.

[0058] (4) Combine filtrate A and filtrate B, keep the pressure at -0.08 Mpa, concentrate under reduced pressure at 60 °C to a relative density of 1.3 and without alcohol smell, and perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, under a vacuum degree of 12 Pa, dry at a temperature of -40 °C to 40 °C, and the drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h) to obtain the mixed extract S2, pulverize and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0059] Example 4

[0060] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke, 15 parts of Taraxacum sinicum Kitag. and 5 parts of Abelmoschus manihot (L.) Medic. by mass.

[0061] The preparation method is as follows:

[0062] (1) Weigh each raw material in the composition according to the ratio, wash, dry, pulverize and pass through a 60-mesh sieve to obtain the mixed powder S1.

[0063] (2) Add 60% ethanol to S1 according to the material-liquid ratio of 1 g: 20 mL, add pectinase and cellulase according to the material-liquid ratio of 1 g: 100 mL (the mass ratio of pectinase to cellulase is 2:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), perform ultrasonic extraction (power 600 W) at 50 °C for 1.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (8000 r / min, 15 min, 20 °C), filter, collect the supernatant to obtain filtrate A and the filter residue.

[0064] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at a material-liquid ratio of 1 g: 10 mL, keep the hot water temperature at 80 °C, extract for 1 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (8000 r / min, 15 min, 20 °C), filter, collect the supernatant to obtain filtrate B.

[0065] (4) Combine filtrate A and filtrate B, maintain the pressure at -0.10 Mpa, and concentrate under reduced pressure at 50°C to a relative density of 1.25 and without alcohol smell. Then perform vacuum freeze-drying (pre-freeze at -80°C for 24 h, and after pre-freezing, conduct drying at a temperature ranging from -40°C to 40°C under a vacuum of 10 Pa. The drying process is to dry for 2 h for every 10°C increase, and the total drying time is 18 h), to obtain the mixed extract S2. Crush and sieve through a 60-mesh sieve to obtain the hypoglycemic composition.

[0066] Example 5

[0067] A hypoglycemic composition is made from 25 parts of Jerusalem artichoke, 10 parts of Taraxacum sinicum Kitag, and 10 parts of Abelmoschus manihot (L.) Medic. by mass.

[0068] The preparation method is as follows:

[0069] (1) Weigh each raw material in the composition according to the proportion, wash, dry, crush, and sieve through a 60-mesh sieve to obtain the mixed powder S1.

[0070] (2) Add 70% ethanol to S1 according to the solid-liquid ratio of 1 g:30 mL, and add pectinase and cellulase according to the solid-liquid ratio of 2 g:100 mL (the mass ratio of pectinase to cellulase is 1:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, and pH = 4). Ultrasonic extract (power 600 W) at 60°C for 1 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (8000 r / min, 15 min, 20°C) and filter, collect the supernatant to obtain filtrate A and the filter residue.

[0071] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction according to the solid-liquid ratio of 1 g:15 mL, keep the hot water temperature at 90°C, extract for 2 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (8000 r / min, 15 min, 20°C) and filter, collect the supernatant to obtain filtrate B.

[0072] (4) Combine filtrate A and filtrate B, maintain the pressure at -0.08 Mpa, and concentrate under reduced pressure at 60°C to a relative density of 1.25 and without alcohol smell. Then perform vacuum freeze-drying (pre-freeze at -80°C for 24 h, and after pre-freezing, conduct drying at a temperature ranging from -40°C to 40°C under a vacuum of 8 Pa. The drying process is to dry for 2 h for every 10°C increase, and the total drying time is 18 h), to obtain the mixed extract S2. Crush and sieve through a 60-mesh sieve to obtain the hypoglycemic composition.

[0073] Example 6

[0074] A hypoglycemic composition is made of 30 parts of Jerusalem artichoke, 10 parts of Taraxacum sinicum Kitag. and 10 parts of Abelmoschus manihot (L.) Medic. by mass.

[0075] The preparation method is as follows:

[0076] (1) Weigh each raw material in the composition according to the proportion, wash, dry, crush and pass through a 70-mesh sieve to obtain a mixed powder S1.

[0077] (2) Add 70% ethanol to S1 according to the solid-liquid ratio of 1 g:20 mL, add pectinase and cellulase according to the solid-liquid ratio of 3 g:100 mL (the mass ratio of pectinase to cellulase is 2:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), ultrasonically extract (power 600 W) at 70 °C for 0.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool and centrifuge (7000 r / min, 20 min, 25 °C) and filter, collect the supernatant to obtain filtrate A and filter residue.

[0078] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction according to the solid-liquid ratio of 1 g:15 mL, keep the hot water temperature at 80 °C, extract for 1 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool and centrifuge (7000 r / min, 20 min, 25 °C) and filter, collect the supernatant to obtain filtrate B.

[0079] (4) Combine filtrate A and filtrate B, keep the pressure at -0.06 Mpa, concentrate under reduced pressure at 50 °C to a relative density of 1.25 and without alcohol smell, and perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, under the condition of a vacuum degree of 12 Pa, dry at a temperature of -40 °C to 40 °C, and the drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h) to obtain a mixed extract S2, crush and pass through a 70-mesh sieve to obtain the hypoglycemic composition.

[0080] Example 7

[0081] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke, 10 parts of Taraxacum sinicum Kitag. and 10 parts of Abelmoschus manihot (L.) Medic. by mass.

[0082] The preparation method is as follows:

[0083] (1) Weigh each raw material in the composition according to the proportion, wash, dry, crush and pass through a 70-mesh sieve to obtain a mixed powder S1.

[0084] (2) Add 80% ethanol to S1 according to the solid-liquid ratio of 1 g:10 mL, add pectinase and cellulase at the solid-liquid ratio of 2 g:100 mL (the mass ratio of pectinase to cellulase is 2:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), ultrasonically extract (power 600 W) at 50 °C for 1.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (7000 r / min, 20 min, 25 °C), filter, collect the supernatant to obtain filtrate A and the filter residue.

[0085] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at the solid-liquid ratio of 1 g:10 mL, keep the hot water temperature at 90 °C, extract for 1.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (7000 r / min, 20 min, 25 °C), filter, collect the supernatant to obtain filtrate B.

[0086] (4) Combine filtrate A and filtrate B, keep the pressure at -0.08 Mpa, concentrate under reduced pressure at 50 °C to a relative density of 1.35 and without alcohol smell, and perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, under a vacuum of 8 Pa, dry at a temperature of -40 °C to 40 °C, and the drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h) to obtain the mixed extract S2, pulverize and pass through a 70-mesh sieve to obtain the hypoglycemic composition.

[0087] Example 8

[0088] A hypoglycemic composition is made of 25 parts of Jerusalem artichoke, 10 parts of Taraxacum sinicum Kitag. and 15 parts of Abelmoschus manihot (L.) Medic. by mass.

[0089] The preparation method is as follows:

[0090] (1) Weigh each raw material in the composition according to the proportion, wash, dry, pulverize and then pass through a 70-mesh sieve to obtain the mixed powder S1.

[0091] (2) Add 60% ethanol to S1 according to the solid-liquid ratio of 1 g:30 mL, add pectinase and cellulase at the solid-liquid ratio of 1 g:100 mL (the mass ratio of pectinase to cellulase is 2:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), ultrasonically extract (power 600 W) at 70 °C for 0.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (7000 r / min, 20 min, 25 °C), filter, collect the supernatant to obtain filtrate A and the filter residue.

[0092] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at a solid-liquid ratio of 1 g:10 mL, keep the hot water temperature at 70 °C, extract for 2 h, filter, collect the filtrate, repeat the above steps for the filter residue (with the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (7000 r / min, 20 min, 25 °C), filter, collect the supernatant to obtain filtrate B.

[0093] (4) Combine filtrate A and filtrate B, keep the pressure at -0.06 Mpa, concentrate under reduced pressure at 55 °C to a relative density of 1.25 and without alcohol smell, and perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, under a vacuum of 12 Pa, dry at a temperature of -40 °C to 40 °C, and the drying process is to dry for 2 h for every 10 °C increase, and the total drying time is 18 h) to obtain the mixed extract S2, pulverize, and pass through a 70-mesh sieve to obtain the hypoglycemic composition.

[0094] Example 9

[0095] A hypoglycemic composition is made of 30 parts of Jerusalem artichoke, 10 parts of Taraxacum sinicum Kitag, and 15 parts of Abelmoschus manihot (L.) Medic. by mass.

[0096] The preparation method is as follows:

[0097] (1) Weigh each raw material in the composition according to the ratio, wash, dry, pulverize, and pass through a 70-mesh sieve to obtain the mixed powder S1.

[0098] (2) Add 80% ethanol to S1 according to a solid-liquid ratio of 1 g:20 mL, add pectinase and cellulase at a solid-liquid ratio of 3 g:100 mL (the mass ratio of pectinase to cellulase is 3:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), perform ultrasonic extraction (power 600 W) at 70 °C for 1 h, filter, collect the filtrate, repeat the above steps for the filter residue (with the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (7000 r / min, 20 min, 25 °C), filter, collect the supernatant to obtain filtrate A and the filter residue.

[0099] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at a solid-liquid ratio of 1 g:10 mL, keep the hot water temperature at 70 °C, extract for 1 h, filter, collect the filtrate, repeat the above steps for the filter residue (with the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool, centrifuge (7000 r / min, 20 min, 25 °C), filter, collect the supernatant to obtain filtrate B.

[0100] (4) Combine filtrate A and filtrate B, maintain the pressure at -0.08 Mpa, and concentrate under reduced pressure at 50 °C until the relative density reaches 1.35 and there is no alcohol smell. Then, perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, and after pre-freezing, carry out drying at a vacuum degree of 10 Pa and a temperature range of -40 °C to 40 °C. The drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h) to obtain the mixed extract S2. Crush it and pass through a 70-mesh sieve to obtain the hypoglycemic composition.

[0101] Example 10

[0102] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke, 20 parts of Taraxacum sinicum Kitag., and 5 parts of Abelmoschus manihot (L.) Medic. by mass.

[0103] The preparation method is as follows:

[0104] (1) Weigh each raw material in the composition according to the ratio, wash, dry, crush, and then pass through a 70-mesh sieve to obtain the mixed powder S1.

[0105] (2) Add 60% ethanol to S1 according to the solid-liquid ratio of 1 g:10 mL, and add pectinase and cellulase according to the solid-liquid ratio of 3 g:100 mL (the mass ratio of pectinase to cellulase is 3:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, and pH = 4). Ultrasonic extract (power 600 W) at 60 °C for 1 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool, and then centrifuge (7000 r / min, 20 min, 25 °C) and filter to collect the supernatant to obtain filtrate A and the filter residue.

[0106] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction according to the solid-liquid ratio of 1 g:15 mL, keep the hot water temperature at 80 °C, extract for 2 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool, and then centrifuge (7000 r / min, 20 min, 25 °C) and filter to collect the supernatant to obtain filtrate B.

[0107] (4) Combine filtrate A and filtrate B, maintain the pressure at -0.10 Mpa, and concentrate under reduced pressure at 60 °C until the relative density reaches 1.35 and there is no alcohol smell. Then, perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, and after pre-freezing, carry out drying at a vacuum degree of 8 Pa and a temperature range of -40 °C to 40 °C. The drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h) to obtain the mixed extract S2. Crush it and pass through a 70-mesh sieve to obtain the hypoglycemic composition.

[0108] Example 11

[0109] A hypoglycemic composition is made of 25 parts of Jerusalem artichoke, 20 parts of Taraxacum sinicum Kitag. and 10 parts of Abelmoschus manihot (L.) Medic. by mass.

[0110] The preparation method is as follows:

[0111] (1) Weigh each raw material in the composition according to the ratio, wash, dry, pulverize and pass through a 60-mesh sieve to obtain a mixed powder S1.

[0112] (2) Add 70% ethanol to S1 according to the solid-liquid ratio of 1 g:10 mL, add pectinase and cellulase according to the solid-liquid ratio of 1 g:100 mL (the mass ratio of pectinase and cellulase is 1:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), ultrasonically extract (power 600 W) at 60 °C for 0.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, centrifuge (9000 r / min, 10 min, 15 °C) after cooling, filter, collect the supernatant to obtain filtrate A and filter residue.

[0113] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction according to the solid-liquid ratio of 1 g:5 mL, keep the hot water temperature at 70 °C, extract for 2 h, filter, collect the filtrate, repeat the above steps for the filter residue (with the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, centrifuge (9000 r / min, 10 min, 15 °C) after cooling, filter, collect the supernatant to obtain filtrate B.

[0114] (4) Combine filtrate A and filtrate B, keep the pressure at -0.06 Mpa, concentrate under reduced pressure at 60 °C to a relative density of 1.35 and without alcohol smell, vacuum freeze-dry (pre-freeze at -80 °C for 24 h, after pre-freezing, under the condition of a vacuum degree of 12 Pa, dry at a temperature of -40 °C to 40 °C, and the drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h) to obtain a mixed extract S2, pulverize and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0115] Example 12

[0116] A hypoglycemic composition is made of 30 parts of Jerusalem artichoke, 15 parts of Taraxacum sinicum Kitag. and 15 parts of Abelmoschus manihot (L.) Medic. by mass.

[0117] The preparation method is as follows:

[0118] (1) Weigh each raw material in the composition according to the ratio, wash, dry, pulverize and pass through a 60-mesh sieve to obtain a mixed powder S1.

[0119] (2) Add 80% ethanol to S1 according to the solid-liquid ratio of 1 g: 30 mL, add pectinase and cellulase at the solid-liquid ratio of 3 g: 100 mL (the mass ratio of pectinase to cellulase is 3:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), and perform ultrasonic extraction (power 600 W) at 50 °C for 1.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, centrifuge after cooling (9000 r / min, 10 min, 15 °C), filter, collect the supernatant, and obtain filtrate A and the filter residue.

[0120] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at the solid-liquid ratio of 1 g: 10 mL, keep the hot water temperature at 70 °C, extract for 1.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, centrifuge after cooling (9000 r / min, 10 min, 15 °C), filter, collect the supernatant, and obtain filtrate B.

[0121] (4) Combine filtrate A and filtrate B, keep the pressure at -0.08 Mpa, concentrate under reduced pressure at 60 °C to a relative density of 1.35 and without alcohol smell, and perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, under the condition of a vacuum degree of 10 Pa, dry at a temperature of -40 °C to 40 °C, and the drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h), obtain the mixed extract S2, pulverize and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0122] Example 13

[0123] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke, 20 parts of Taraxacum sinicum Kitag. and 15 parts of Abelmoschus manihot (L.) Medic. by mass.

[0124] The preparation method is as follows:

[0125] (1) Weigh each raw material in the composition according to the proportion, wash, dry, pulverize and pass through a 60-mesh sieve to obtain the mixed powder S1.

[0126] (2) Add 60% ethanol to S1 according to the solid-liquid ratio of 1 g: 20 mL, add pectinase and cellulase at the solid-liquid ratio of 2 g: 100 mL (the mass ratio of pectinase to cellulase is 1:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), and perform ultrasonic extraction (power 600 W) at 50 °C for 0.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, centrifuge after cooling (9000 r / min, 10 min, 15 °C), filter, collect the supernatant, and obtain filtrate A and the filter residue.

[0127] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at a solid-liquid ratio of 1 g: 15 mL, keep the hot water temperature at 80 °C, extract for 1 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (9000 r / min, 10 min, 15 °C) and filter, collect the supernatant to obtain filtrate B.

[0128] (4) Combine filtrate A and filtrate B, keep the pressure at -0.10 Mpa, concentrate under reduced pressure at 50 °C to a relative density of 1.3 and without alcohol smell, and conduct vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, under a vacuum degree of 8 Pa, conduct drying at a temperature of -40 °C to 40 °C, and the drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h) to obtain the mixed extract S2, pulverize and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0129] Example 14

[0130] A hypoglycemic composition is made of 25 parts of Jerusalem artichoke, 20 parts of Taraxacum sinicum Kitag. and 15 parts of Abelmoschus manihot (L.) Medic. by mass.

[0131] The preparation method is as follows:

[0132] (1) Weigh each raw material in the composition according to the proportion, wash, dry, pulverize and then pass through a 60-mesh sieve to obtain the mixed powder S1.

[0133] (2) Add 80% ethanol to S1 according to a solid-liquid ratio of 1 g: 10 mL, add pectinase and cellulase at a solid-liquid ratio of 2 g: 100 mL (the mass ratio of pectinase to cellulase is 1:1, the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4), conduct ultrasonic extraction (power 600 W) at 70 °C for 1.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (9000 r / min, 10 min, 15 °C) and filter, collect the supernatant to obtain filtrate A and the filter residue.

[0134] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at a solid-liquid ratio of 1 g: 5 mL, keep the hot water temperature at 90 °C, extract for 1 h, filter, collect the filtrate, repeat the above steps for the filter residue (with exactly the same parameter settings), and obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (9000 r / min, 10 min, 15 °C) and filter, collect the supernatant to obtain filtrate B.

[0135] (4) Combine filtrate A and filtrate B, maintain the pressure at -0.08 Mpa, and concentrate under reduced pressure at 60 °C until the relative density reaches 1.35 and there is no alcohol smell. Then perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, and after pre-freezing, conduct drying at a temperature ranging from -40 °C to 40 °C under a vacuum of 12 Pa. The drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h), to obtain the mixed extract S2. Crush it and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0136] Example 15

[0137] A hypoglycemic composition is made of 30 parts of Jerusalem artichoke, 10 parts of Taraxacum sinicum Kitag, and 5 parts of Abelmoschus manihot (L.) Medic. by mass.

[0138] The preparation method is as follows:

[0139] (1) Weigh each raw material in the composition according to the proportion, wash, dry, crush, and then pass through a 60-mesh sieve to obtain the mixed powder S1.

[0140] (2) Add 60% ethanol to S1 according to the solid-liquid ratio of 1 g:30 mL, and add pectinase and cellulase according to the solid-liquid ratio of 3 g:100 mL (the mass ratio of pectinase to cellulase is 3:1, and the enzyme activities are 30000 U / g and 10000 U / g respectively, pH = 4). Ultrasonic extract (power 600 W) at 70 °C for 1.5 h, filter, collect the filtrate, repeat the above steps for the filter residue (with the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (9000 r / min, 10 min, 15 °C) and filter, collect the supernatant to obtain filtrate A and the filter residue.

[0141] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction according to the solid-liquid ratio of 1 g:15 mL, keep the hot water temperature at 90 °C, extract for 2 h, filter, collect the filtrate, repeat the above steps for the filter residue (with the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool and then centrifuge (9000 r / min, 10 min, 15 °C) and filter, collect the supernatant to obtain filtrate B.

[0142] (4) Combine filtrate A and filtrate B, maintain the pressure at -0.10 Mpa, and concentrate under reduced pressure at 50 °C until the relative density reaches 1.25 and there is no alcohol smell. Then perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, and after pre-freezing, conduct drying at a temperature ranging from -40 °C to 40 °C under a vacuum of 10 Pa. The drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h), to obtain the mixed extract S2. Crush it and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0143] Comparative Example 1

[0144] (1) Weigh 1 kg of Jerusalem artichoke, wash, dry, pulverize it, and sieve it through a 60-mesh sieve to obtain Jerusalem artichoke powder.

[0145] The preparation methods of steps (2) to (4) are the same as those in Example 1, except that Jerusalem artichoke powder is used to replace the mixed powder S1.

[0146] Comparative Example 2

[0147] (1) Weigh 1 kg of Taraxacum sinicum Kitag., wash, dry, pulverize it, and sieve it through a 60-mesh sieve to obtain Taraxacum sinicum Kitag. powder.

[0148] The preparation methods of steps (2) to (4) are the same as those in Example 1, except that Taraxacum sinicum Kitag. powder is used to replace the mixed powder S1.

[0149] Comparative Example 3

[0150] (1) Weigh 1 kg of Abelmoschus manihot (L.) Medic., wash, dry, pulverize it, and sieve it through a 60-mesh sieve to obtain Abelmoschus manihot (L.) Medic. powder.

[0151] The preparation methods of steps (2) to (4) are the same as those in Example 1, except that Abelmoschus manihot (L.) Medic. powder is used to replace the mixed powder S1.

[0152] Comparative Example 4

[0153] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke and 10 parts of Taraxacum sinicum Kitag. by mass.

[0154] The preparation method is the same as that in Example 1.

[0155] Comparative Example 5

[0156] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke and 5 parts of Abelmoschus manihot (L.) Medic. by mass.

[0157] The preparation method is the same as that in Example 1.

[0158] Comparative Example 6

[0159] A hypoglycemic composition is made of 10 parts of Taraxacum sinicum Kitag. and 5 parts of Abelmoschus manihot (L.) Medic. by mass.

[0160] The preparation method is the same as that in Example 1.

[0161] Comparative Example 7

[0162] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke, 10 parts of dandelion and 5 parts of Abelmoschus manihot (L.) Medic. by mass.

[0163] The preparation method is the same as that in Example 1.

[0164] Comparative Example 8

[0165] A hypoglycemic composition is made of 20 parts of Jerusalem artichoke, 10 parts of Taraxacum sinicum Kitag. and 5 parts of Abelmoschus manihot (L.) Medic. by mass.

[0166] The preparation method is the same as that of Example 1, except that the enzymatic hydrolysis process is removed:

[0167] (1) Weigh each raw material in the composition according to the ratio, wash, dry, crush and pass through a 60-mesh sieve to obtain a mixed powder S1.

[0168] (2) Add 70% ethanol to S1 according to the solid-liquid ratio of 1 g:20 mL, and perform ultrasonic extraction (power 600 W) at 60 °C for 1 h, filter, and collect the filtrate; repeat the above steps for the filter residue (with exactly the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool and centrifuge (8000 r / min, 15 min, 20 °C), filter, and collect the supernatant to obtain filtrate A and the filter residue.

[0169] (3) Weigh the filter residue finally obtained in step (2), add hot water for extraction at a solid-liquid ratio of 1 g:10 mL, keep the hot water temperature at 70 °C, extract for 1.5 h, filter, and collect the filtrate; repeat the above steps for the filter residue (with exactly the same parameter settings), and extract to obtain the filtrate. Combine the filtrates extracted twice, cool and centrifuge (8000 r / min, 15 min, 20 °C), filter, and collect the supernatant to obtain filtrate B.

[0170] (4) Combine filtrate A and filtrate B, keep the pressure at -0.08 Mpa, and concentrate under reduced pressure at 55 °C to a relative density of 1.25 and without alcohol smell, and perform vacuum freeze-drying (pre-freeze at -80 °C for 24 h, after pre-freezing, under a vacuum degree of 10 Pa, dry at a temperature of -40 °C to 40 °C, and the drying process is to dry for 2 h every time the temperature rises by 10 °C, and the total drying time is 18 h) to obtain a mixed extract S2, crush and pass through a 60-mesh sieve to obtain the hypoglycemic composition.

[0171] Test Example 1 Determination of the change in the content of functional components

[0172] Determination object: Take equal amounts of the samples obtained in Examples 1 to 15 and Comparative Examples 1 to 8.

[0173] Determination method: The content of phenolic acid compounds is determined according to the method of NY / T 3949-2021, the content of flavonoid compounds is determined according to the method of NY / T3950-2021, the total sugar content is determined by colorimetry using the phenol-sulfuric acid method, and the reducing sugar content is determined by the 3,5-dinitrosalicylic acid method. The difference between the total sugar content and the reducing sugar content is used to characterize the polysaccharide content in the sample, and it is expressed as a mass ratio (%).

[0174] Polysaccharide mass ratio (%) = total sugar mass ratio - reducing sugar mass ratio.

[0175] The test results are shown in Table 1.

[0176] Table 1 Test results of the content of functional components in different treatment groups

[0177] Group Phenolic acid content (%) Flavonoid content (%) Polysaccharide content (%) Example 1 15.87±1.50a 12.98±1.14a 45.22±1.04a Example 2 13.75±1.61a 10.52±0.85bc 42.52±1.74ab Example 3 14.21±1.64a 12.10±1.73ab 42.68±2.08ab Example 4 13.48±2.23a 11.65±0.98ab 42.62±1.05ab Example 5 13.86±1.14a 10.68±0.98bc 41.45±2.24ab Example 6 14.03±1.61a 10.45±1.32bc 41.07±2.55b Example 7 13.28±2.06a 10.64±1.12bc 41.95±1.02ab Example 8 13.52±1.60a 11.59±1.35ab 41.13±2.53b Example 9 12.92±2.49a 10.55±1.36bc 42.12±1.91ab Example 10 13.12±1.39a 11.04±1.39ab 41.28±2.37ab Example 11 14.61±1.88a 11.28±1.55ab 43.10±2.87ab Example 12 13.57±1.24a 11.50±1.34ab 42.08±2.25ab Example 13 13.24±2.26a 11.49±0.92ab 42.35±4.22ab Example 14 13.15±1.40a 11.34±1.12ab 41.26±1.98ab Example 15 13.57±1.75a 12.02±0.93ab 42.00±2.27ab Control Example 1 8.61±0.92b 7.12±1.15d 37.13±1.43c Control Example 2 8.54±1.38b 8.71±0.26cd 34.53±1.33cd Control Example 3 9.06±0.04b 8.24±0.31d 33.12±2.98d Control Example 4 8.99±0.81b 8.20±0.94d 37.25±0.64c Control Example 5 7.22±1.00b 8.49±0.47d 37.51±1.54c Control Example 6 9.49±0.46b 8.33±1.17d 35.15±0.88cd Control Example 7 10.06±0.95b 8.51±0.45d 36.29±1.16cd Control Example 8 8.44±0.89b 7.04±0.30d 33.96±1.52cd

[0178] Note: Different letters in the same column indicate significant differences at the 0.05 level, P < 0.05.

[0179] The results show that the content of phenolic acids in the composition prepared by the method of the present invention can reach up to 15.87%, the flavonoid content can reach 12.98%, and the polysaccharide content can reach 45.22%.

[0180] Test Example 2

[0181] Using the hypoglycemic composition obtained in Example 1, study the hypoglycemic effect of the composition of the present invention on type 2 diabetic rats.

[0182] (1) 140 SPF-grade male SD rats at 8 weeks of age, with a body weight of (200 ± 20) g, were provided by Beijing Huafukang Biotechnology Co., Ltd., and the animal production license number: SCXK (Jing) 2019 - 0008. This experiment was approved by the Experimental Animal Ethics Committee of North China University of Science and Technology (201521). The animals were housed in the animal house of North China University of Science and Technology, and the breeding conditions were: room temperature (22 ± 2) °C, relative humidity 40% - 60%, and illuminated from 8:00 to 16:00 every day. Every 6 rats were placed in a cage, with free access to water and fed a normal diet. After one week of adaptive feeding, the experiment was carried out.

[0183] (2) Modeling and grouping

[0184] Randomly select 10 rats as the normal control group (i.e., the blank control group) and feed them a normal diet. The remaining 130 rats were fed a high-sugar and high-fat diet as the model group. After feeding for 7 days and fasting for 12 hours, the rats in the model group were injected with 50 mg / kg STZ solution by intraperitoneal injection, and the rats in the blank control group were intraperitoneally injected with an equal volume of distilled water. The rats were closely observed for 7 days, and the fasting blood glucose value (FBG) of the rats was measured by tail tip blood sampling on the 3rd and 7th days. If the FBG was ≥ 11.1 mmol / L in both measurements, it was considered that the modeling was successful. For rats with FPG < 11.1 mmol / L, they were intraperitoneally injected with STZ (50 mg / kg) again. The successfully modeled rats were randomly divided into 13 groups: model control group, positive control group, experimental low-dose group, experimental medium-dose group, experimental high-dose group, comparative example 1 group, comparative example 2 group, comparative example 3 group, comparative example 4 group, comparative example 5 group, comparative example 6 group, comparative example 7 group, comparative example 8 group, with 10 rats in each group.

[0185] During the experiment, the blank control group was intragastrically administered with distilled water and fed with normal feed; the model control group was intragastrically administered with distilled water and fed with high-sugar and high-fat feed; the remaining groups were intragastrically administered with the corresponding solvents at 10 mL / kg to the rats in each modeling group and fed with high-sugar and high-fat feed. For 28 days, the rats were allowed to eat and drink freely. Each group was intragastrically administered according to Table 2.

[0186] Table 2 Grouping of animal experiments

[0187]

[0188]

[0189] (3) Data detection and analysis

[0190] Body weight measurement: The body weights of all rats were weighed on a balance on the 0th, 7th, 14th, and 28th days of drug administration.

[0191] Fasting blood glucose measurement: Rats were randomly selected regularly to detect fasting blood glucose. On the 0th, 7th, 14th, and 28th days of drug administration, after fasting for 12 h, blood was taken from the tail vein of the rats, and the fasting blood glucose value was detected by the blood glucose meter test strip method.

[0192] Determination of blood lipid metabolism indexes: After 28 days of drug administration, the rats in each experimental group were anesthetized, blood was taken from the abdominal aorta, left to stand for 60 min, and centrifuged at 3000 r / min for 15 min to separate the serum. The contents of total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL-C), and low-density lipoprotein (LDL-C) were determined by the kit method.

[0193] SPSS 23.0 software was used for data processing. One-way analysis of variance was used for the differences between groups. A significant difference was considered when P < 0.05, and no significant difference was considered when P > 0.05. The data results were expressed as mean ± standard deviation (x±s).

[0194] The effects of each group on the body weight of type 2 diabetic rats are shown in Table 3, the effects on the fasting blood glucose of type 2 diabetic rats are shown in Table 4, and the effects on the lipid metabolism of type 2 diabetic rats are shown in Table 5.

[0195] Table 3 Effects of the composition of the present invention on the body weight of type 2 diabetic rats

[0196]

[0197]

[0198] Table 4 Effects of the composition of the present invention on the fasting blood glucose of type 2 diabetic rats

[0199]

[0200] Table 5 Effects of the composition of the present invention on lipid metabolism in type 2 diabetic rats

[0201]

[0202]

[0203] Note: # indicates P < 0.05 compared with the blank control group, and * indicates P < 0.05 compared with the model control group.

[0204] As can be seen from Table 3, before the intervention, there was no significant difference in the body weights of the rats in the model control group, positive control group, experimental group, and comparative example group (P > 0.05). After the intervention, the body weight of the rats in the blank control group continued to increase steadily. Compared with the blank control group, the body weights of the model control group, positive control group, experimental group, and comparative example group decreased, and with the continuous presence of hyperglycemia, significant differences were shown among the groups (P < 0.05). Compared with the model control group, the high, medium, and low dose groups of the experiment had a significant effect on the body weight of the rats (P < 0.05), indicating that the composition described in the present invention can significantly improve the weight loss of experimental diabetic rats.

[0205] As can be seen from Table 4, compared with the blank control group, the fasting blood glucose level of the diabetic rats after modeling increased significantly (P < 0.05) and remained at a high blood glucose level, indicating that the diabetic rat model was successfully established. During the entire administration period, the average blood glucose level of the blank control group was stable at about 5 mmol / L, while the blood glucose of the rats in the model control group gradually increased (P < 0.05). Compared with the model control group, the high, medium, and low doses of the experiment could all reduce the blood glucose level of the rats, and the hypoglycemic effects of the medium and high dose groups of the experiment were the most significant, with the blood glucose reduction rates being 17.76% and 20.65% respectively, and there were significant differences among the groups (P < 0.05). This indicates that the blood glucose-lowering composition of the present invention can effectively reduce the blood glucose level of rats. Compared with the model control group, the blood glucose of the rats in Comparative Example Groups 1-8 decreased to a certain extent, but the blood glucose reduction effect was not as significant as that of the experimental group. It shows that at the same dose, the effect of the three-component mixed extract is better than that of the Jerusalem artichoke extract, Taraxacum sinicum Kitag. extract, Abelmoschus manihot extract, or the mixed extracts of any two of them.

[0206] As can be seen from Table 5, compared with the blank control group, the levels of serum TC, TG, and LDL-C in the model control group of rats were significantly increased (P<0.05), while the level of HDL-C was significantly decreased (P<0.05). Compared with the model control group, the levels of TC and TG in the positive control group and the high, medium, and low-dose experimental groups were significantly decreased (P<0.05), the level of HDL-C was significantly increased (P<0.05), and the levels of LDL-C in the positive control group and the high-dose experimental group were significantly decreased (P<0.05). It shows that the hypoglycemic composition of the present invention can effectively reduce the levels of total cholesterol, triglyceride, and low-density lipoprotein in the serum of rats, and increase the level of high-density lipoprotein. Among them, the high-dose experimental group has the most significant effect on regulating the lipid metabolism level of rats.

[0207] In summary, the hypoglycemic composition of the present invention can not only improve the weight loss of experimental diabetic rats, but also effectively reduce the blood glucose level of rats and regulate the lipid metabolism level, which has significant significance for the prevention and treatment of diabetes and its complications.

[0208] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and modifications can be made, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A hypoglycemic composition, characterized in that: The invention is prepared from the following raw materials by weight: 20-30 parts of Jerusalem artichoke, 10-20 parts of alkali soil dandelion and 5-15 parts of golden sunflower.

2. The method for preparing the hypoglycemic composition according to claim 1, characterized in that: The following steps are involved: The raw material powder is mixed with ethanol to obtain a mixed system; pectinase and cellulase are added to the mixed system, ultrasonic extraction is performed, the extract is centrifuged and filtered to obtain filter residue and filtrate A; the filter residue is subjected to hot water extraction, the extract is centrifuged and filtered to obtain filtrate B; the filtrate A and the filtrate B are combined, and the results are concentrated under reduced pressure, freeze-dried in vacuum, and pulverized to obtain a hypoglycemic composition.

3. The preparation method according to claim 2, characterized in that: The material-liquid ratio of the raw material powder to ethanol is 1g:10-30mL; the ethanol is 60%-80% ethanol.

4. The preparation method according to claim 2, characterized in that: In terms of the mixed system, the added amount of the pectinase and the cellulase is 1-5 g:100 mL; the mass ratio of the pectinase and the cellulase is 1-3:1; the enzyme activity of the pectinase is 25000-35000 U / g, and the enzyme activity of the cellulase is 5000-15000 U / g.

5. The preparation method according to claim 2, characterized in that: The power of the ultrasonic extraction is 500-700W, the temperature is 50-70°C, and the time is 0.5-1.5h.

6. The preparation method according to claim 2, characterized in that: During the hot water extraction, the material-liquid ratio of the filter residue to water is 1g:5-15mL.

7. The preparation method according to claim 2, characterized in that: The hot water extraction temperature is 70-90° C. and the time is 1-2 hours.

8. The preparation method according to claim 2, characterized in that: The centrifugal conditions are: 7000-9000 r / min, 10-20 min, 18-25°C.

9. The preparation method according to claim 2, characterized in that: The conditions of the reduced pressure concentration are: -0.06 to -0.10 MPa, 50 to 60° C.; the reduced pressure concentration is performed to a relative density of 1.25 to 1.

35.

10. Use of the hypoglycemic composition according to claim 1 or the preparation method according to any one of claims 2 to 9 in preparing a drug for preventing and / or treating diabetes.

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