Traditional Chinese medicine compound composition as well as preparation method and application thereof

Abstract

The invention discloses a traditional Chinese medicine compound composition as well as a preparation method and application thereof, and belongs to the technical field of veterinary traditional Chinese medicine preparations. The traditional Chinese medicine compound composition comprises the following raw materials: 20-60 parts of jasminum elongatum, 10-30 parts of ovateleaf holly bark, 30-60 parts of szechwan chinaberry fruit, 10-30 parts of folium artemisiae argyi, 20-40 parts of Chinese pulsatilla root, 20-60 parts of hairyvein agrimony, 10-50 parts of acanthopanax root and 10-50 In addition, the invention further provides a preparation method of the traditional Chinese medicine compound composition, and the preparation method comprises the following steps: mixing the raw materials, further mixing with water, and soaking; decocting after soaking, and filtering to obtain decoction and dregs; and concentrating the decoction to obtain extract. In addition, the invention further provides application of the traditional Chinese medicine compound composition in preparation of medicines for preventing and / or treating coccidiosis or poultry breeding. The traditional Chinese medicine compound composition provided by the invention can effectively prevent and treat coccidiosis.

Description

Technical Field

[0001] The present invention relates to the technical field of veterinary traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine compound composition, a preparation method thereof and an application thereof. Background Art

[0002] Chicken coccidiosis is a protozoal disease caused by one or more Eimeria spp. parasitizing in intestinal epithelial cells. This disease is widely distributed, has a complex life cycle, and is difficult to eradicate. It can cause varying degrees of diarrhea, bloody stools, listlessness, lethargy, slow weight gain, reduced feed conversion rate, and decreased production performance in poultry. The incidence rate is as high as 50% - 70%, the mortality rate is 20% - 30%, and in severe cases, it can reach 80%, causing serious economic losses to the global poultry industry.

[0003] Currently, chemical drugs are still an important means for preventing and treating chicken coccidiosis, but the problems of drug resistance and drug residues caused by long-term use are becoming increasingly serious. There is an urgent need to develop new anti-coccidial drugs. However, the research and development cycle of a new chemically synthesized anti-coccidial drug with high safety, high efficiency and low toxicity is long and the upfront investment is huge. Seeking natural and safe alternatives has gradually become the research focus for preventing and treating this disease.

[0004] Traditional Chinese herbs have the advantages of low drug residues, small toxic and side effects, and being less likely to produce drug resistance. They have played an important role in promoting animal growth, inhibiting bacteria, killing bacteria, antiviral, improving product quality, anti-stress, and improving the livestock house environment, etc., which conforms to the concept of green and healthy breeding and has broad prospects.

[0005] According to the theory of evidence-based medicine, the subordinate syndrome of coccidiosis is the dysfunction of the spleen after the accumulation of dampness and heat in the large intestine, manifested as bloody diarrhea. Currently, most traditional Chinese herbs for treating coccidiosis are mainly composed of traditional Chinese herbs with the effects of expelling parasites and killing insects, clearing heat and drying dampness, cooling blood and detoxifying, and are combined with traditional Chinese herbs with the effects of replenishing qi and nourishing blood, promoting qi circulation and relieving pain, stopping bleeding, strengthening the spleen and promoting digestion, antibacterial and antiviral, etc. They treat both the symptoms and the root causes, achieving a balance between ascending and descending. They not only achieve the anti-coccidial effect, but also can promote animal growth, improve immunity, repair the damage to the cecum tissue caused by coccidiosis, and accelerate the recovery of intestinal tissue, etc.

[0006] Although some existing traditional Chinese medicine compositions can treat coccidiosis, the treatment effect is not ideal yet, and there is still a need to develop a traditional Chinese medicine composition that can effectively prevent or treat coccidiosis. Summary of the Invention

[0007] The purpose of the present invention is to overcome the above technical deficiencies, provide a traditional Chinese medicine compound composition, a preparation method thereof and an application thereof, and solve the technical problem of how to effectively prevent or treat coccidiosis in the prior art.

[0008] In order to achieve the above technical purpose, the technical solution of the present invention provides a Chinese medicine compound composition. The raw materials are calculated by weight and include: 20-60 parts of Gnaphalium wilfordii, 10-30 parts of Scutellaria baicalensis, 30-60 parts of Toosendan fruit, 10-30 parts of Artemisia argyi, 20-40 parts of Pulsatilla scutellariae, 20-60 parts of Agrimoniae scabra, 10-50 parts of Acanthopanax senticosus and 10-50 parts of Astragalus membranaceus.

[0009] In any embodiment, the raw materials are calculated by weight and include: 40 parts of Gnaphalium wilfordii, 20 parts of Rhizoma Cibotii, 60 parts of Toosendan fruit, 20 parts of Artemisia argyi, 30 parts of Pulsatilla striata, 30 parts of Agrimoniae scabra, 10 parts of Acanthopanax senticosus and 10 parts of Astragalus membranaceus.

[0010] In addition, the present invention also provides a method for preparing the above-mentioned Chinese medicine compound composition, comprising the following steps: grinding each raw material into powder, and then sieving and mixing to obtain a Chinese medicine compound composition powder.

[0011] In addition, the present invention also provides a method for preparing the above-mentioned Chinese medicine compound composition, comprising the following steps:

[0012] S1, mixing the raw materials, and then further mixing with water and soaking;

[0013] S2, decocting after soaking in step S1, filtering to obtain the decoction and the residue;

[0014] S3, concentrating the decoction to obtain an extract.

[0015] In any embodiment, before step S3, the method further includes: decocting the medicinal residue obtained in step S2 for 1 to 2 times, mixing the liquid obtained in each decocting with the decoction of step S2, and then performing the concentration treatment of step S3.

[0016] In any embodiment, in step S1, the amount of water used is 7-9 times the total weight of the raw materials; and / or, in step S1, the soaking time is 40-50 minutes; and / or, in step S2, the decoction time is 1-1.5 hours.

[0017] In any embodiment, in step S2, the dregs are decocted for 30 to 40 minutes.

[0018] In any embodiment, in step S3, the extract is concentrated to a relative density of 1.20 to 1.30 at 60°C.

[0019] In any embodiment, after step S3, the method further includes adding a proper amount of dextrin to the extract, and mixing the mixture to form granules.

[0020] In addition, the present invention also provides an application of the above-mentioned traditional Chinese medicine compound composition or the traditional Chinese medicine compound composition prepared by the above-mentioned preparation method in the preparation of drugs for preventing and / or treating coccidiosis, or an application in poultry farming.

[0021] Compared with the prior art, the beneficial effects of the present invention include: through the design of an experimental model, the present invention explores the prevention and treatment effects of drugs such as Jasminum elongatum and Ilex rotunda on chicken coccidiosis, and finally develops a traditional Chinese medicine compound composition made of Jasminum elongatum, Ilex rotunda, Melia toosendan, Artemisia argyi, Pulsatilla chinensis, Agrimonia pilosa, Eleutherococcus senticosus, and Astragalus membranaceus. When combined, these herbs have a strong effect of killing and expelling parasites and also have the effect of strengthening the healthy qi and eliminating pathogenic factors, and can effectively prevent and treat coccidiosis. Detailed implementation manners

[0022] The "range" disclosed in the present application is defined in the form of a lower limit and an upper limit. A given range is defined by selecting a lower limit and an upper limit, and the selected lower limit and upper limit define the boundary of a specific range. The range defined in this way can include or not include the end values, and can be combined arbitrarily, that is, any lower limit can be combined with any upper limit to form a range. For example, if ranges of 60-120 and 80-110 are listed for a specific parameter, ranges of 60-110 and 80-120 are also contemplated. In addition, if the minimum range values 1 and 2 are listed, and if the maximum range values 3, 4, and 5 are listed, the following ranges are all contemplated: 1-3, 1-4, 1-5, 2-3, 2-4, and 2-5. In the present application, unless otherwise specified, the numerical range "a-b" represents an abbreviated representation of any real number combination between a and b, where a and b are both real numbers. For example, the numerical range "0-5" means that all real numbers between "0-5" have been fully listed herein, and "0-5" is only an abbreviated representation of these numerical combinations. In addition, when it is stated that a certain parameter is an integer ≥2, it is equivalent to disclosing that the parameter is, for example, the integer 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, etc.

[0023] If there is no special description, the "including" and "comprising" mentioned in the present application mean open-ended or can also be closed-ended. For example, the "including" and "comprising" can mean that other components not listed can also be included or comprised, or can only include or comprise the listed components.

[0024] If there is no special description, in the present application, the term "or" is inclusive. For example, the phrase "A or B" means "A, B, or both A and B". More specifically, any of the following conditions satisfies the condition "A or B": A is true (or exists) and B is false (or does not exist); A is false (or does not exist) while B is true (or exists); or both A and B are true (or exist).

[0025] This specific embodiment provides a traditional Chinese medicine compound composition. Calculated by weight parts, the raw materials include: 20-60 parts of Jasminum elongatum, 10-30 parts of Ilex rotunda, 30-60 parts of Melia toosendan, 10-30 parts of Artemisia argyi, 20-40 parts of Pulsatilla chinensis, 20-60 parts of Agrimonia pilosa, 10-50 parts of Acanthopanax senticosus, and 10-50 parts of Astragalus membranaceus.

[0026] In some embodiments, calculated by weight parts, the raw materials include: 40 parts of Jasminum elongatum, 20 parts of Ilex rotunda, 60 parts of Melia toosendan, 20 parts of Artemisia argyi, 30 parts of Pulsatilla chinensis, 30 parts of Agrimonia pilosa, 10 parts of Acanthopanax senticosus, and 10 parts of Astragalus membranaceus.

[0027] This specific embodiment also provides a preparation method of the above traditional Chinese medicine compound composition, including the following steps: grinding each raw material into powder, and then passing through a sieve and mixing to obtain a powder of the traditional Chinese medicine compound composition. In some embodiments, pass through a No. 5 sieve and mix evenly in a mixing tank.

[0028] In addition, this specific embodiment also provides a preparation method of the above traditional Chinese medicine compound composition, including the following steps:

[0029] S1. Mix each raw material, and then further mix with water and soak for 40-50 min; the amount of water used is 7-9 times the total weight of each raw material;

[0030] S2. After soaking in step S1, decoct for 1-1.5 h, and filter to obtain a decoction and medicinal residues;

[0031] S3. Concentrate the decoction to an extract with a relative density of 1.20-1.30 at 60 °C.

[0032] In some embodiments, decoct the medicinal residues obtained in step S2 for 1-2 times, and mix the liquid obtained by decocting for 30-40 min each time with the decoction in step S2, and then perform the concentration treatment in step S3.

[0033] In some embodiments, after step S3, it further includes: adding dextrin to the extract according to a mass ratio of the extract to dextrin of 1:2, and mixing to make granules.

[0034] This specific embodiment also provides an application of the traditional Chinese medicine compound composition or the traditional Chinese medicine compound composition prepared by the above preparation method in the preparation of drugs for preventing and / or treating coccidiosis, or its application in poultry farming.

[0035] In order to make the purpose, technical solution and advantages of the present invention clearer, the following further elaborates on the present invention in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0036] In the present invention, reference is made to "some embodiments", "the present embodiment", and examples, etc., which describe subsets of all possible embodiments. However, it is understood that "some embodiments" can be the same subset or different subsets of all possible embodiments, and can be combined with each other without conflict.

[0037] If similar descriptions such as "first / second" appear in the application documents, the following explanation shall be added. In the following description, the terms "first / second / third" only distinguish similar objects and do not represent a specific order for the objects. It is understood that "first / second / third" can be interchanged in a specific order or sequence when permitted, so that the embodiments described herein can be implemented in an order other than that described herein.

[0038] In the present embodiment, the term "and / or" only describes the association relationship of associated objects, indicating that there can be three relationships. For example, object A and / or object B can represent three situations: object A exists alone, object A and object B exist simultaneously, and object B exists alone.

[0039] The following describes the embodiments of the present application. The embodiments described below are exemplary and are only used to explain the present application and should not be construed as a limitation of the present application. For those embodiments where specific technologies or conditions are not indicated, they shall be carried out according to the technologies or conditions described in the literature in this field or according to the product specifications. For reagents or instruments whose manufacturers are not indicated, they are all conventional products that can be obtained through commercial purchase.

[0040] Embodiment 1

[0041] In this embodiment, a traditional Chinese medicine composite composition is proposed. Weigh each drug raw material according to the weight ratio: 40 g of Jasminum elongatum, 20 g of Ilex rotunda, 60 g of Toosendan fruit, 20 g of Artemisia argyi, 30 g of Pulsatilla chinensis, 30 g of Agrimonia pilosa, 10 g of Eleutherococcus senticosus, and 10 g of Astragalus membranaceus.

[0042] Preparation method of powder: Weigh each weight portion of the traditional Chinese medicine raw materials, remove impurities respectively, pulverize them into powder, pass through a No. 5 sieve, and fully mix them evenly in a mixing tank to make a powder.

[0043] Preparation method of granule:

[0044] Weigh each weight portion of the traditional Chinese medicine raw materials, soak them in 8 times the total weight of all the medicinal materials in water for 40 min, then decoct them for 1 hour after soaking, filter to obtain the first decoction and the medicinal residues;

[0045] Add 7 times the total weight of all the medicinal materials in water to the medicinal residues and decoct them for 30 min, filter to obtain the second decoction;

[0046] Add 7 times the total weight of all the medicinal materials in water to the medicinal residues and decoct them for 30 min, filter to obtain the third decoction;

[0047] Combine all the decoctions after three - time decocting, and carry out filtration treatment. Concentrate the filtrate to an extract with a relative density of 1.20 at 60 °C.

[0048] Add dextrin to the extract according to the mass ratio of extract to dextrin of 1:2. After mixing evenly, make granules, and after drying, package them according to 1000 g / bag to obtain the granule agent.

[0049] The usage method of the drug in this example is as follows: for the prevention and treatment of chicken coccidiosis, for powder, mix it with feed: add 10 kg of the drug of the present invention to every 1 ton of feed and use it continuously for 7 days; for granule, mix it with drinking water: add 1 kg of the drug of the present invention to every 1 ton of water and use it continuously for 7 days.

[0050] Example 2

[0051] This example provides a traditional Chinese medicine composite composition. Weigh each drug raw material according to the weight ratio: 20 g of Jasminum elongatum, 30 g of Ilex rotunda Thunb., 30 g of Melia toosendan Sieb. et Zucc., 30 g of Artemisia argyi Levl. et Vant., 40 g of Pulsatilla chinensis (Bunge) Regel, 60 g of Agrimonia pilosa Ledeb., 50 g of Acanthopanax senticosus (Rupr. et Maxim.) Harms, 50 g of Astragalus membranaceus (Fisch.) Bunge.

[0052] Prepare and use according to the method of Example 1 to obtain powder and granule agents respectively.

[0053] Example 3

[0054] This example provides a traditional Chinese medicine composite composition. Weigh each drug raw material according to the weight ratio: 50 g of Jasminum elongatum, 10 g of Ilex rotunda Thunb., 40 g of Melia toosendan Sieb. et Zucc., 10 g of Artemisia argyi Levl. et Vant., 20 g of Pulsatilla chinensis (Bunge) Regel, 20 g of Agrimonia pilosa Ledeb., 20 g of Acanthopanax senticosus (Rupr. et Maxim.) Harms, 30 g of Astragalus membranaceus (Fisch.) Bunge.

[0055] Prepare and use according to the method of Example 1 to obtain powder and granule agents respectively.

[0056] Example 4

[0057] This example provides a traditional Chinese medicine composite composition. Weigh each drug raw material according to the weight ratio: 60 g of Jasminum elongatum, 10 g of Ilex rotunda Thunb., 50 g of Melia toosendan Sieb. et Zucc., 20 g of Artemisia argyi Levl. et Vant., 20 g of Pulsatilla chinensis (Bunge) Regel, 40 g of Agrimonia pilosa Ledeb., 40 g of Acanthopanax senticosus (Rupr. et Maxim.) Harms, 40 g of Astragalus membranaceus (Fisch.) Bunge.

[0058] Prepare and use according to the method of Example 1 to obtain powder and granule agents respectively.

[0059] Comparative Example 1

[0060] Weigh each drug raw material according to the weight ratio: 60 g of Melia toosendan Sieb. et Zucc., 20 g of Artemisia argyi Levl. et Vant., 60 g of Agrimonia pilosa Ledeb., 40 g of Acanthopanax senticosus (Rupr. et Maxim.) Harms, 40 g of Astragalus membranaceus (Fisch.) Bunge. Prepare and use according to the method of Example 1 to obtain powder and granule agents respectively.

[0061] Comparative Example 2

[0062] Weigh each drug raw material according to the weight ratio: 60 g of Toosendan Fruit, 20 g of Artemisia Argyi Leaf, 30 g of Pulsatilla Root, 30 g of Agrimony Herb, 40 g of Acanthopanax Senticosus, 40 g of Astragalus Root, and prepare and use them according to the method of Example 1 to obtain powder and granule respectively.

[0063] Comparative Example 3

[0064] Weigh each drug raw material according to the weight ratio: 40 g of Jasminum Lanceolarium Root, 20 g of Ilex Rotunda Thunb., 60 g of Toosendan Fruit, 20 g of Artemisia Argyi Leaf, 30 g of Agrimony Herb, 25 g of Acanthopanax Senticosus, 25 g of Astragalus Root, and prepare and use them according to the method of Example 1 to obtain powder and granule respectively.

[0065] Comparative Example 4

[0066] Weigh each drug raw material according to the weight ratio: 40 g of Jasminum Lanceolarium Root, 20 g of Ilex Rotunda Thunb., 20 g of Artemisia Argyi Leaf, 30 g of Pulsatilla Root, 30 g of Agrimony Herb, 40 g of Acanthopanax Senticosus, 40 g of Astragalus Root, and prepare and use them according to the method of Example 1 to obtain powder and granule respectively.

[0067] Comparative Example 5

[0068] Weigh each drug raw material according to the weight ratio: 40 g of Jasminum Lanceolarium Root, 20 g of Ilex Rotunda Thunb., 60 g of Toosendan Fruit, 30 g of Pulsatilla Root, 30 g of Agrimony Herb, 20 g of Acanthopanax Senticosus, 20 g of Astragalus Root, and prepare and use them according to the method of Example 1 to obtain powder and granule respectively.

[0069] Comparative Example 6

[0070] Weigh each drug raw material according to the weight ratio: 40 g of Jasminum Lanceolarium Root, 20 g of Ilex Rotunda Thunb., 30 g of Pulsatilla Root, 30 g of Agrimony Herb, 50 g of Acanthopanax Senticosus, 50 g of Astragalus Root, and prepare and use them according to the method of Example 1 to obtain powder and granule respectively.

[0071] Experimental Example 1 Verification of the Prevention and Treatment Effect of Traditional Chinese Medicine Compound on Artificially Induced Coccidiosis in Chickens

[0072] 1.1 Experimental Animals

[0073] SPF White Leghorn chickens hatched from SPF eggs and raised in an avian isolator until 12 days old can freely eat and drink, and are given the same photoperiod, relative humidity and room temperature.

[0074] 1.2 Experimental Methods and Judgment Criteria

[0075] The experimental chicks were raised until 12 days old and randomly divided into 23 groups, with 10 chicks in each group. Among them, groups S1 - S14 and S18 - S23 were the groups administered with different formula drugs and challenged with parasites; group S15 was the group not challenged with parasites and not administered with drugs; group S16 was the group challenged with parasites and not administered with drugs; group S17 was the diclazuril control group. The traditional Chinese medicine compound experimental groups S1 - S7 and the powder groups S18 - S20 were administered by mixing into the feed at a dose of 10 g / kg for 7 consecutive days; the traditional Chinese medicine compound experimental groups S8 - S14 and the granule groups S21 - S23 were administered by drinking water at a dose of 1 g / L for 7 consecutive days; the diclazuril control group S17 was administered by mixing into the feed at a dose of 0.2 g / kg for 7 consecutive days; 2 days after drug administration, except for group S15 (the group not challenged with parasites and not administered with drugs), each chicken in the remaining groups was orally inoculated with 4.0×10 4 sporulated oocysts of Eimeria tenella HB - 10 strain. Among them, the sporulated oocysts of Eimeria tenella HB - 10 strain were preserved by Wuhan Keqian Biology Co., Ltd.

[0076] Table 1 Experimental grouping and scheme

[0077] Serial number Group Number of chickens / each Dosing dose <![CDATA[Inoculum size (10 4 )]]> S1 Powder of Example 1 10 10 g / kg feed 4 S2 Powder of Comparative Example 1 10 10 g / kg feed 4 S3 Powder of Comparative Example 2 10 10 g / kg feed 4 S4 Powder of Comparative Example 3 10 10 g / kg feed 4 S5 Powder of Comparative Example 4 10 10 g / kg feed 4 S6 Powder of Comparative Example 5 10 10 g / kg feed 4 S7 Powder of Comparative Example 6 10 10 g / kg feed 4 S8 Granule of Example 1 10 1 g / L water 4 S9 Granule of Comparative Example 1 10 1 g / L water 4 S10 Granule of Comparative Example 2 10 1 g / L water 4 S11 Granule of Comparative Example 3 10 1 g / L water 4 S12 Granule of Comparative Example 4 10 1 g / L water 4 S13 Granule of Comparative Example 5 10 1 g / L water 4 S14 Granule of Comparative Example 6 10 1 g / L water 4 S15 Group without attacking insects and without medicating 10 / / S16 Group with attacking insects and without medicating 10 / 4 S17 Diclazuril control group 10 0.2 g / kg feed 4

[0078] Table 2 Experimental grouping and scheme of Examples 2 - 4

[0079]

[0080]

[0081] During the experiment, the mental state of the experimental chickens in each group, the death situation and bloody stool situation of the chickens caused by coccidiosis, etc. were observed and recorded. On the 8th day after challenging with parasites, the surviving chickens in each group were weighed and then all dissected.

[0082] According to the relative weight gain rate, survival rate, lesion value and oocyst value of the experimental chickens in each group, the anti - coccidial index (ACI) was calculated according to the calculation formula of Merck Company, USA.

[0083] Relative weight gain rate: The fasting weight of the chickens at the time of challenging with parasites was used as the initial weight, and the weight at the end of the experiment was used as the final weight to calculate the relative weight gain rate. The specific calculation method was: average weight gain = average final weight - average initial weight, relative weight gain rate (%) = (average weight gain of the experimental chickens in each group / average weight gain of the negative control group) × 100%.

[0084] Survival rate: The number of dead chickens after infection with coccidia in each group was counted, and the survival rate was calculated, that is, survival rate = (number of surviving chickens in each group at the end of the experiment / total number of chickens in this group at the start of the experiment) × 100%.

[0085] Lesion value: After the experiment, the chickens were dissected and the ceca were taken out. The cecal lesions were scored according to the scoring criteria of Johnson and Reid, and the lesion value was calculated. Lesion value = average cecal lesion score of each group × 10.

[0086] Oocyst value: Fecal samples of chickens in each experimental group were collected on the 6th, 7th, and 8th days after inoculation with parasites. The fecal oocysts were counted using a hemocytometer, and the oocyst ratio was calculated based on the number of oocysts in each experimental group. Oocyst ratio (%) = number of oocysts in each infected group / number of oocysts in the group inoculated with parasites without drug administration × 100%. When the oocyst ratio is 0 - 1%, the oocyst value is 0; when it is 2% - 25%, the oocyst value is 5; when it is 26% - 50%, the oocyst value is 10; when it is 51% - 75%, the oocyst value is 20; when it is 76% - 100%, the oocyst value is 40.

[0087] ACI = (relative weight gain rate + survival rate) - (cecal lesion value + oocyst value). ACI ≤ 120 is ineffective, ACI = 120 - 160 is low - effective, ACI = 160 - 180 is medium - effective, and ACI ≥ 180 is high - effective.

[0088] Among them, deaths caused by non - coccidiosis need to be excluded, that is, dead chickens within 1 - 3 days after inoculation. The lesions were examined by dissection. Those without specific coccidiosis lesions were excluded and defined as non - coccidiosis deaths. Deaths may be caused by factors such as stress and improper inoculation.

[0089] 1.3 Test results

[0090] 1.3.1 Clinical symptoms

[0091] Clinical observation found that the experimental chickens in the group without inoculation and without drug administration had normal food intake, water intake, activity level, mental state and feces. The experimental chickens in the group inoculated with parasites without drug administration showed abnormal mental state, decreased food intake, huddled together without moving, messy feathers, and bloody stools. The mental state, food intake and bloody stools in the diclazuril control group, each administration group of examples and each administration group of comparative examples were improved to varying degrees.

[0092] 1.3.2 Pathological autopsy conditions

[0093] Through the autopsy of the experimental chickens, it was found that the organs of the group without inoculation and without drug administration were normal and there were no macroscopic lesions.

[0094] In the group inoculated with parasites without drug administration, the ceca were thickened and shortened, with a large number of bleeding points and bloody contents, and some had cecal cores. The cecal lesions in the diclazuril control group, each administration group of examples and each administration group of comparative examples were reduced, the bloody contents decreased, and no obvious macroscopic lesions were found in other organs.

[0095] 1.3.3 Results of each index

[0096] Record and process the data of the average weight gain, relative weight gain rate, survival rate, lesion value, and oocyst value of each group of experimental chickens. The test results are shown in Tables 3 and 4.

[0097] Table 3 Test Results

[0098]

[0099]

[0100] Table 4

[0101]

[0102] The results in Tables 3 and 4 show that: compared with the group challenged with parasites without medication, the ACI values of the diclazuril control group and all experimental groups were significantly increased, confirming the effectiveness of the treatment effects of each experimental group. The following important findings can be obtained through comparison between groups: (1) Among the comparative groups containing heat-clearing drugs, the ACI value of the combination of Jasminum elongatum and Ilex rotunda (comparative example 3 group) was increased by 8.1% compared with that of traditional Pulsatilla chinensis (comparative example 2 group), reflecting the synergistic advantage of the new heat-clearing compatibility. And from comparative example 1, it can be seen that heat-clearing drugs play an important role in the treatment of chicken coccidiosis; (2) Through comparison with comparative example 6, it can be seen that anthelmintic and insecticidal drugs play an important role in the treatment of chicken coccidiosis, and the ACI value increased by 14.3% - 41.0% after the combined use of Toosendan fruit and Artemisia argyi, with better effects; (3) The ACI value of the granule group in Example 1 can reach as high as 193.79, and its curative effect reaches the high-efficiency level standard (ACI≥180), which is significantly higher than the other comparative examples, indicating that the components show a synergistic effect after being prepared according to a specific ratio and can effectively prevent and treat chicken coccidiosis. In addition, the relative bioavailability of the granule of Example 1 was significantly improved compared with the traditional powder. The granule and powder of Example 4 also had good treatment effects, but were weaker than Example 1.

[0103] The specific implementation manners of the present invention described above do not constitute a limitation to the protection scope of the present invention. Any other corresponding changes and deformations made according to the technical concept of the present invention should be included in the protection scope of the claims of the present invention.

Claims

1. A Chinese medicine compound composition, characterized in that: The raw materials are calculated by weight and include: 20-60 parts of Radix Gnaphalii, 10-30 parts of Rhizoma Cibotii, 30-60 parts of Toosendan fruit, 10-30 parts of Artemisia argyi, 20-40 parts of Pulsatilla striata, 20-60 parts of Herba Agrimoniae, 10-50 parts of Acanthopanax senticosus and 10-50 parts of Radix Astragali.

2. The Chinese medicine compound composition according to claim 1, characterized in that: The raw materials are calculated by weight and include: 40 parts of Radix Gnaphalii, 20 parts of Rhizoma Cibotii, 60 parts of Fructus Toosendan, 20 parts of Folium Artemisiae Argyi, 30 parts of Pulsatillae Scutellariae, 30 parts of Herba Agrimoniae, 10 parts of Acanthopanax Senticosus and 10 parts of Radix Astragali.

3. A method for preparing the Chinese medicine compound composition according to any one of claims 1 to 2, characterized in that: The following steps are involved: The raw materials are ground into powder, and then sieved and mixed to obtain a Chinese medicine compound composition powder.

4. A method for preparing the Chinese medicine compound composition according to any one of claims 1 to 2, characterized in that: The following steps are involved: S1, mixing the raw materials, and then further mixing with water and soaking; S2, decocting after soaking in step S1, filtering to obtain the decoction and the residue; S3, concentrating the decoction to obtain an extract.

5. The method for preparing the Chinese medicine compound composition according to claim 4, characterized in that: Before step S3, the method further includes: decocting the medicinal residue obtained in step S2 for 1 to 2 times, mixing the liquid obtained in each decocting with the decoction in step S2, and then performing concentration treatment in step S3.

6. The method for preparing the Chinese medicine compound composition according to claim 4, characterized in that: In step S1, the amount of water used is 7-9 times the total weight of the raw materials; and / or, in step S1, the soaking time is 40-50 minutes; and / or, in step S2, the decoction time is 1-1.5 hours.

7. The method for preparing the Chinese medicine compound composition according to claim 5, characterized in that: In step S2, the dregs are boiled for 30 to 40 minutes.

8. The method for preparing the Chinese medicine compound composition according to claim 4, characterized in that: In step S3, the extract is concentrated to a relative density of 1.20 to 1.30 at 60°C.

9. The method for preparing the Chinese medicine compound composition according to claim 4, characterized in that: After step S3, the method further includes adding dextrin to the extract and mixing the mixture to form granules.

10. Use of the Chinese medicinal compound composition according to any one of claims 1 to 2 or the Chinese medicinal compound composition prepared by the preparation method according to any one of claims 4 to 9 in preparing a drug for preventing and / or treating coccidiosis, or in poultry breeding.

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