Plaster for promoting circulation and relieving fatigue and preparation method thereof

Abstract

The invention belongs to the field of plaster preparation, and particularly relates to a plaster for promoting circulation and relieving fatigue and a preparation method thereof. A viscous compound solution formed by waterborne polyurethane and polyvinyl alcohol through hydrogen bond interaction is crosslinked with silk fibroin to obtain a gel base material, the circulation-promoting extract is adsorbed, and the prepared circulation-promoting pain-relieving micelles have the effects of promoting circulation and relieving pain; amide groups on molecular chains of polyacrylamide and carboxyl of sodium chenodeoxycholate are subjected to electrostatic interaction, the formed compound solution and the fatigue relieving solution are subjected to freeze spray drying, the compound solution forms a film with certain mechanical strength and flexibility, and the obtained penetration-promoting fatigue relieving particles can be quickly and efficiently absorbed by skin; the soothing anti-allergy agent is mixed with the circulation-promoting pain-relieving micelles and the permeation-promoting fatigue-relieving particles, so that the prepared circulation-promoting fatigue-relieving plaster is remarkable in effect, stable and lasting in drug effect after being used and stored for a long time, free of irritation to skin and not prone to falling off.

Description

Technical Field

[0001] The present invention belongs to the field of plaster preparation, and specifically refers to a plaster for promoting blood circulation and relieving fatigue and its preparation method. Background Art

[0002] Plaster patches are a traditional Chinese medicine external preparation with a long history and wide application. By mixing drugs with suitable matrices to form pastes and coating them on backing materials for skin application, they can play local or systemic therapeutic effects. The drug components in the plaster can penetrate through the skin into the subcutaneous tissue to exert anti-inflammatory and analgesic effects. For example, plasters containing blood-activating and stasis-dispelling components can be used for traumatic injuries, joint pains, etc., and can also penetrate through the skin to dispel wind and cold, warm the meridians and relieve pain.

[0003] When using plaster patches, it is necessary to pay attention to cleaning the skin before application. If necessary, it can be wiped with alcohol or ginger slices. Select the appropriate type of plaster according to the condition to avoid allergic reactions. The application time should not be too long, generally 6 - 12 hours. If skin redness, itching or other discomforts occur, the use should be stopped immediately. In short, as a traditional Chinese medicine external preparation, plaster patches have the advantages of simple preparation, convenient use, and remarkable curative effects, and are widely used in the treatment of diseases such as pain, inflammation, and rheumatism.

[0004] Currently, the existing plaster preparation technologies have the following problems: First, the drug components of existing plaster patches are difficult to be effectively released and absorbed, resulting in poor efficacy in promoting blood circulation and relieving physical fatigue; Second, after long-term storage or use, the drug components of existing plaster patches may become ineffective and the efficacy is unstable; Third, some plaster patches have insufficient breathability and are prone to cause skin discomfort during use. Due to the complex composition of the plaster, some products have allergic and irritating reactions. Summary of the Invention

[0005] In view of the above situation, to overcome the defects of the existing technology, the present invention provides a plaster for promoting blood circulation and relieving fatigue and its preparation method. To solve the problems of poor efficacy, unstable efficacy, invalidation after storage, and irritation to the skin of existing plasters, the present invention uses a blood circulation-promoting and pain-relieving micelle, a penetration-promoting and fatigue-relieving particle, and a soothing and anti-allergic agent. The prepared plaster for promoting blood circulation and relieving fatigue has remarkable efficacy, stable and long-lasting drug effect, and does not cause irritation to the skin after long-term use.

[0006] To achieve the above object, the technical solution adopted by the present invention is as follows. The present invention provides a plaster for promoting blood circulation and relieving fatigue, and the preparation raw materials of the plaster for promoting blood circulation and relieving fatigue specifically include the following components in parts by weight: 45 - 55 parts of blood circulation-promoting and pain-relieving micelle, 22 - 28 parts of penetration-promoting and fatigue-relieving particle, 1.8 - 2.2 parts of anhydrous betaine, 3.4 - 4.1 parts of cornus officinalis extract, 1.5 - 2 parts of bisabolol.

[0007] Preferably, the raw materials for preparing the blood circulation-promoting and pain-relieving micelles include the following components in parts by weight: 11-14 parts of succinimide, 12-15 parts of 2,6-dimethylpyridine, 8-10 parts of waterborne polyurethane, 11-13 parts of polyvinyl alcohol, 9-14 parts of fibroin, 1.2-1.4 parts of tannic acid, 4-7 parts of apocynum venetum, 8-9 parts of schisandra chinensis, 7-10 parts of notoginseng, 2.3-2.7 parts of eicosapentaenoic acid, and 2.6-2.9 parts of boswellic acid.

[0008] Preferably, the raw materials for preparing the penetration-promoting and fatigue-relieving particles include the following components in parts by weight: 6-9 parts of chenodeoxycholic acid sodium, 11-13 parts of polyacrylamide, 8-10 parts of parsley, 12-16 parts of spinach, 2-2.5 parts of N-acetyl-L-carnitine, 1.5-1.8 parts of salidroside, and 1.4-1.7 parts of theanine.

[0009] Preferably, the method for preparing the blood circulation-promoting and pain-relieving micelles specifically includes the following steps: S1. Put succinimide, 2,6-dimethylpyridine, and 50% ethanol solution into a stirrer with a power of 1.5-1.8 kW, stir at a temperature of 25-33 °C, a stirring speed of 300-320 r / min, and a stirring duration of 10-15 min for mixing and dissolving to obtain a vasodilating solution; S2. Put waterborne polyurethane, polyvinyl alcohol, and ultrapure water into a constant-temperature stirrer with a power of 2.2-2.6 kW, stir at a constant temperature of 80-90 °C for a constant-temperature stirring duration of 3-4.5 h, and a constant-temperature stirring speed of 1000 r / min for stirring reaction to form a viscous composite solution; S3. Put the viscous composite solution prepared in S2, fibroin, tannic acid, and dimethyl sulfoxide into a reaction kettle with a power of 2.2-2.6 kW, react at a temperature of 40 °C for a reaction duration of 4-6 h, and a reaction speed of 300-500 r / min. After cross-linking reaction, a gel substrate is obtained; S4. Put apocynum venetum, schisandra chinensis, and notoginseng into a crusher with a power of 1.5-1.8 kW, crush at a temperature of 23-28 °C for a crushing duration of 15-20 min, and a crushing speed of 3500-4500 r / min. After crushing, put it into an ultrasonic extractor with a power of 1.3-1.6 kW together with 70% ethanol solution. The extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40-45 °C, and the extraction duration is 8-13 min for extraction to obtain a blood circulation-promoting extract; S5. Put the circulation-promoting extract, eicosapentaenoic acid, and boswellic acid prepared in S4 into the vasodilating solution prepared in S1, stir at a temperature of 25 - 33°C, a stirring speed of 300 - 320 r / min, and a stirring duration of 15 - 20 min to obtain a circulation-promoting and pain-relieving solution by mixing evenly. S6. Add the gel substrate prepared in S3 to the circulation-promoting and pain-relieving solution prepared in S5, stir at a temperature of 25 - 33°C, a stirring speed of 300 - 320 r / min, and a stirring duration of 25 - 30 min to obtain a circulation-promoting and pain-relieving micelle by sufficient adsorption.

[0010] Further, in S1, the mass fraction of succinimide in the 50% ethanol solution is 3 - 5%, and in S2, the mass fraction of waterborne polyurethane in ultrapure water is 23 - 30%.

[0011] Further, in S3, the mass fraction of fibroin in dimethyl sulfoxide is 10%, and in S4, the material-liquid ratio of notoginseng to 70% ethanol solution is 1:3 g / mL.

[0012] Preferably, the preparation method of the penetration-promoting and fatigue-relieving particles specifically includes the following steps: L1. Put sodium chenodeoxycholate, polyacrylamide, and ultrapure water into a constant-temperature stirrer with a power of 1.6 - 1.9 kW, stir at a constant temperature of 30 - 40°C, a constant stirring speed of 300 - 500 r / min, and a constant stirring duration of 4 - 6 h to carry out a reaction to form a complex solution. L2. Put parsley, spinach, and ultrapure water into a grinder with a power of 1.8 - 2.4 kW, grind at a temperature of 85 - 90°C, a grinding duration of 35 - 40 min, and a grinding speed of 18000 - 20000 r / min. After grinding, filter with a sterile filter cloth, and put it together with N-acetyl-L-carnitine, salidroside, and theanine into a stirrer with a power of 1.5 - 1.8 kW, stir at a temperature of 25 - 33°C, a stirring speed of 300 - 320 r / min, and a stirring duration of 10 - 15 min to obtain a fatigue-relieving solution. L3. Put the fatigue-relieving solution prepared in L2 into the complex solution prepared in L1, stir at a constant temperature of 25 - 30°C, a constant stirring speed of 300 - 500 r / min, and a constant stirring duration of 20 - 30 min. After mixing evenly, put it into a vacuum freeze nano spray dryer with a power of 2.3 - 2.8 kW, a vacuum degree of -0.05 MPa, a freeze nano spray drying temperature of -40°C, a freeze nano spray drying particle size of 100 nm, and a freeze nano spray drying duration of 2.5 - 3 h. After freeze spray drying, obtain the penetration-promoting and fatigue-relieving particles.

[0013] Further, in L1, the mass fraction of sodium chenodeoxycholate in ultrapure water is 15-18%, and the mass fraction of polyacrylamide in ultrapure water is 17-21%.

[0014] Further, in L2, the feed ratio of parsley to ultrapure water is 5:1.5 g / mL.

[0015] The present invention also provides a method for preparing a promoting blood circulation and relieving fatigue plaster, which specifically includes the following steps: Step 1: Put anhydrous betaine, cornus officinalis extract, bisabolol, and 50% ethanol solution into a stirrer with a power of 1.5-1.8 kW, stir at a temperature of 30-35 °C, a stirring speed of 400-600 r / min, and a stirring duration of 10-15 min to fully dissolve and obtain a soothing and anti-allergic agent. Step 2: Put the promoting blood circulation and pain-relieving micelle into the soothing and anti-allergic agent prepared in Step 1, stir at a temperature of 32-36 °C, a stirring speed of 400-600 r / min, and a stirring duration of 20-30 min. After sufficient adsorption, obtain a gel substrate. Step 3: Put the promoting penetration and relieving fatigue particles into the gel substrate prepared in Step 2, use an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20000 r / min, a stirring temperature of 25 °C, and a stirring duration of 10 min. After uniform dispersion, obtain a promoting blood circulation and relieving fatigue mixture. Step 4: Coat the promoting blood circulation and relieving fatigue mixture prepared in Step 3 on a medical adhesive tape with a coating thickness of 1-1.3 mm to obtain a promoting blood circulation and relieving fatigue plaster.

[0016] Preferably, in Step 1, the mass fraction of anhydrous betaine in 50% ethanol solution is 2.3%.

[0017] The beneficial effects achieved by the present invention are as follows: The present invention uses polyvinyl alcohol and aqueous polyurethane with good biocompatibility, which are dissolved in ultrapure water. The urethane groups in the aqueous polyurethane and the hydroxyl groups in the polyvinyl alcohol interact through hydrogen bonds to form a stable network structure. The resulting viscous complex has good flexibility and strong adhesion. The viscous complex solution is mixed with fibroin, and under the catalytic action of tannic acid, the hydroxyl groups in the polyvinyl alcohol react with the amino or carboxyl groups in the protein to form cross-links through esterification or amidation reactions. The isocyanate groups in the aqueous polyurethane can react with the amino or hydroxyl groups in the protein to form stable covalent bonds, obtaining a gel substrate with a high specific surface area. Apocynum venetum, Schisandra chinensis, and Panax notoginseng are pulverized and then extracted to obtain a circulation-promoting extract that can enhance myocardial contractility and slow down the heart rate. Succinimide and 2,6-dimethylpyridine block the influx of extracellular calcium ions into cells by binding to L-type and T-type calcium channels on the cell membrane, reducing the intracellular calcium ion concentration, which leads to smooth muscle relaxation and blood vessel dilation. The two act synergistically to improve the heart's pumping efficiency, improve blood fluidity, and promote blood circulation. Eicosapentaenoic acid promotes skeletal muscle growth and regulates glucose metabolism by inhibiting the production of inflammatory mediators through activating the PI3K / AKT signaling pathway, thereby accelerating muscle recovery. Boswellic acid can inhibit the activation of inflammatory cells and the release of inflammatory factors, reduce muscle inflammation, and relieve muscle pain. The gel substrate fully adsorbs the circulation-promoting and pain-relieving solution, and the resulting circulation-promoting and pain-relieving micelles have a significant effect of promoting blood circulation and relieving pain, with strong adhesion and comfortable use. Sodium chenodeoxycholate has an amphiphilic structure, which can reduce the lipid orderliness of the skin stratum corneum and increase the permeability of the stratum corneum, thereby promoting the transdermal absorption of active ingredients. Polyacrylamide has good film-forming properties. The amide groups on the polyacrylamide molecular chain can undergo electrostatic interactions with the carboxyl groups of sodium chenodeoxycholate to form a complex. Parsley and spinach contain B vitamins and coenzyme Q10, which can reduce the free radicals generated during exercise, reduce the damage of oxidative stress to muscles and nerves, reduce lactic acid accumulation by regulating energy metabolism, and thus relieve muscle fatigue. Synergistic with N-acetyl-L-carnitine, salidroside, and L-theanine, the resulting fatigue-relieving solution can regulate neurotransmitter levels and the body's stress response, effectively relieve nerve fatigue, enhance the body's tolerance to fatigue, reduce the damage of oxidative stress to muscle and nerve cells. The fatigue-relieving solution is mixed with the complex solution and subjected to freeze spray drying. The complex solution forms a film with certain mechanical strength and flexibility, maintaining the stability of the active components in the fatigue-relieving solution. The resulting penetration-promoting and fatigue-relieving particles can be rapidly and efficiently absorbed by the skin;Betaine anhydrous, Cornus officinalis extract, and Bisabolol are fully dissolved to obtain a soothing and anti-allergic agent that promotes the repair of skin cells, enhances the skin barrier function, reduces skin inflammatory reactions, and decreases the occurrence of allergic reactions. It is mixed with a circulation-promoting pain-relieving micelle and a penetration-promoting fatigue-relieving particle, and coated on a medical adhesive tape to obtain a circulation-promoting and fatigue-relieving plaster with remarkable efficacy. After long-term use and storage, the drug effect is stable and lasting. Long-term use does not cause irritation to the skin and does not easily fall off. Brief Description of the Drawings

[0018] To more clearly illustrate the technical solutions in the present invention or the prior art, the following will be described in a clear and understandable manner in combination with the drawings. Obviously, the drawings in the following description are only those of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0019] Figure 1 Scanning electron micrograph of the internal structure of the gel substrate described in the present invention; Figure 2 Graph showing the difference in microcirculation blood flow in the auricles of mice in Experimental Example 1 of the present invention Figure 3 Graph showing the number of wheel-drop times of mice in Experimental Example 1 of the present invention; Figure 4 Graph showing the difference in microcirculation blood flow in the auricles of mice after storage in Experimental Example 2 of the present invention; Figure 5 Graph showing the number of wheel-drop times of mice after storage in Experimental Example 2 of the present invention; Figure 6 Finished product diagram of the circulation-promoting and fatigue-relieving plaster described in the present invention. Detailed Description of the Invention

[0020] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in combination with the drawings in the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0021] Unless otherwise defined, all professional and scientific terms used herein have the same meaning as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to the described content can be applied to the present invention. The preferred implementation methods and materials described herein are only for demonstration purposes, but do not limit the content of this application.

[0022] In the experimental methods of the following examples, unless otherwise specified, they are all conventional methods; the test materials and test strains used in the following examples, unless otherwise specified, are all purchased from commercial channels.

[0023] Example 1: This example provides a blood circulation-promoting and fatigue-relieving plaster and its preparation method. The blood circulation-promoting and fatigue-relieving plaster contains the following components in parts by weight: 45 parts of blood circulation-promoting and pain-relieving micelles, 22 parts of penetration-promoting and fatigue-relieving particles, 1.8 parts of anhydrous betaine, 3.4 parts of cornel extract, and 1.5 parts of bisabolol.

[0024] The raw materials for preparing the blood circulation-promoting and pain-relieving micelles include the following components in parts by weight: 11 parts of succinimide, 12 parts of 2,6-dimethylpyridine, 8 parts of waterborne polyurethane, 11 parts of polyvinyl alcohol, 9 parts of fibroin, 1.2 parts of tannic acid, 4 parts of apocynum venetum, 8 parts of schisandra chinensis, 7 parts of notoginseng, 2.3 parts of eicosapentaenoic acid, and 2.6 parts of boswellic acid.

[0025] The raw materials for preparing the penetration-promoting and fatigue-relieving particles include the following components in parts by weight: 6 parts of chenodeoxycholic acid sodium, 11 parts of polyacrylamide, 8 parts of parsley, 12 parts of spinach, 2 parts of N-acetyl-L-carnitine, 1.5 parts of salidroside, and 1.4 parts of L-theanine.

[0026] The preparation method of the blood circulation-promoting and pain-relieving micelles specifically includes the following steps: S1. Put succinimide, 2,6-dimethylpyridine, and 50% ethanol solution into a stirrer with a power of 1.5 kW. The stirring temperature is 25 °C, the stirring speed is 300 r / min, and the stirring time is 10 min for mixing and dissolving to obtain a vasodilating solution. S2. Put waterborne polyurethane, polyvinyl alcohol, and ultrapure water into a constant-temperature stirrer with a power of 2.2 kW. The constant-temperature stirring temperature is 80 °C, the constant-temperature stirring time is 3 h, and the constant-temperature stirring speed is 1000 r / min for stirring reaction to form a viscous complex solution. S3. Put the viscous complex solution prepared in S2, fibroin, tannic acid, and dimethyl sulfoxide into a reaction kettle with a power of 2.2 kW. The reaction temperature is 40 °C, the reaction time is 4 h, and the reaction speed is 300 r / min. After cross-linking reaction, a gel substrate is obtained. S4. Put apocynum venetum, schisandra chinensis, and notoginseng into a crusher with a power of 1.5 kW, with a crushing temperature of 23°C, a crushing duration of 15 min, and a crushing rotation speed of 3500 r / min. After crushing, put it into an ultrasonic extractor with a power of 1.3 kW together with a 70% ethanol solution. The extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 40°C, and the extraction duration is 8 min for extraction to obtain a circulation-promoting extract; S5. Put the circulation-promoting extract prepared in S4, eicosapentaenoic acid, and boswellic acid into the vasodilating solution prepared in S1. The stirring temperature is 25°C, the stirring rotation speed is 300 r / min, and the stirring duration is 15 min to mix evenly to obtain a circulation-promoting and pain-relieving solution; S6. Add the gel substrate prepared in S3 to the circulation-promoting and pain-relieving solution prepared in S5. The stirring temperature is 25°C, the stirring rotation speed is 300 r / min, and the stirring duration is 25 min to fully adsorb to obtain a circulation-promoting and pain-relieving micelle.

[0027] In S1, the mass fraction of succinimide in a 50% ethanol solution is 3%. In S2, the mass fraction of aqueous polyurethane in ultrapure water is 23%.

[0028] In S3, the mass fraction of fibroin in dimethyl sulfoxide is 10%. In S4, the material-liquid ratio of notoginseng to a 70% ethanol solution is 1:3 g / mL.

[0029] The preparation method of the penetration-promoting and fatigue-relieving particles specifically includes the following steps: L1. Put sodium chenodeoxycholate, polyacrylamide, and ultrapure water into a constant-temperature stirrer with a power of 1.6 kW. The constant-temperature stirring temperature is 30°C, the constant-temperature stirring rotation speed is 300 r / min, and the constant-temperature stirring duration is 4 h for reaction to form a complex solution; L2. Put parsley, spinach, and ultrapure water into a pulper with a power of 1.8 kW. The pulping temperature is 85°C, the pulping duration is 35 min, and the pulping rotation speed is 18000 r / min for pulping. Filter it with a sterile filter cloth, and put it into a stirrer with a power of 1.5 kW together with N-acetyl-L-carnitine, salidroside, and theanine. The stirring temperature is 25°C, the stirring rotation speed is 300 r / min, and the stirring duration is 10 min to obtain a fatigue-relieving solution; L3. Put the anti-fatigue solution prepared in L2 into the composite solution prepared in L1. The constant-temperature stirring temperature is 25 °C, the constant-temperature stirring speed is 300 r / min, and the constant-temperature stirring duration is 20 min. After mixing evenly, put it into a vacuum freeze-drying nano spray dryer with a power of 2.3 kW, a vacuum degree of -0.05 MPa, a freeze-drying nano spray drying temperature of -40 °C, a freeze-drying nano spray drying particle size of 100 nm, and a freeze-drying nano spray drying duration of 2.5 h. After freeze spray drying, pro-permeation anti-fatigue particles are obtained.

[0030] In L1, the mass fraction of sodium chenodeoxycholate in ultrapure water is 15%, and the mass fraction of polyacrylamide in ultrapure water is 17%.

[0031] In L2, the material-liquid ratio of parsley to ultrapure water is 5:1.5 g / mL.

[0032] This example also provides a preparation method of a blood circulation-promoting anti-fatigue plaster, which specifically includes the following steps: Step 1. Put anhydrous betaine, cornus officinalis extract, bisabolol, and 50% ethanol solution into a stirrer with a power of 1.5 kW. The stirring temperature is 30 °C, the stirring speed is 400 r / min, and the stirring duration is 10 min. After fully dissolving, a soothing anti-allergy agent is obtained. Step 2. Put the blood circulation-promoting pain-relieving micelle into the soothing anti-allergy agent prepared in Step 1. The stirring temperature is 32 °C, the stirring speed is 400 r / min, and the stirring duration is 20 min. After full adsorption, a gel substrate is obtained. Step 3. Put the pro-permeation anti-fatigue particles into the gel substrate prepared in Step 2. Use an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20000 r / min, a stirring temperature of 25 °C, and a stirring duration of 10 min. After uniform dispersion, a blood circulation-promoting anti-fatigue mixture is obtained. Step 4. Coat the blood circulation-promoting anti-fatigue mixture prepared in Step 3 on a medical adhesive tape with a coating thickness of 1 mm to obtain a blood circulation-promoting anti-fatigue plaster.

[0033] In Step 1, the mass fraction of anhydrous betaine in 50% ethanol solution is 2.3%.

[0034] Example 2: This example provides a blood circulation-promoting anti-fatigue plaster and its preparation method. The blood circulation-promoting anti-fatigue plaster contains the following components in parts by weight: 50 parts of blood circulation-promoting pain-relieving micelle, 25 parts of pro-permeation anti-fatigue particles, 2 parts of anhydrous betaine, 3.8 parts of cornus officinalis extract, and 1.8 parts of bisabolol.

[0035] The raw materials for preparing the blood circulation-promoting and pain-relieving micelles include the following components in parts by weight: 12 parts of succinimide, 13 parts of 2,6-dimethylpyridine, 9 parts of waterborne polyurethane, 12 parts of polyvinyl alcohol, 11 parts of fibroin, 1.3 parts of tannic acid, 5 parts of apocynum venetum, 8.5 parts of schisandra chinensis, 8 parts of notoginseng, 2.5 parts of eicosapentaenoic acid, and 2.7 parts of boswellic acid; The raw materials for preparing the penetration-promoting and fatigue-relieving particles include the following components in parts by weight: 7 parts of chenodeoxycholic acid sodium, 12 parts of polyacrylamide, 9 parts of parsley, 14 parts of spinach, 2.3 parts of N-acetyl-L-carnitine, 1.6 parts of salidroside, and 1.5 parts of L-theanine.

[0036] The preparation method of the blood circulation-promoting and pain-relieving micelles specifically includes the following steps: S1. Put succinimide, 2,6-dimethylpyridine, and 50% ethanol solution into a stirrer with a power of 1.6 kW, stir at a temperature of 28°C, a stirring speed of 310 r / min, and a stirring time of 13 min for mixing and dissolution to obtain a vasodilating solution; S2. Put waterborne polyurethane, polyvinyl alcohol, and ultrapure water into a constant-temperature stirrer with a power of 2.4 kW, stir at a constant temperature of 85°C for 3.8 h, at a constant stirring speed of 1000 r / min for stirring reaction to form a viscous complex solution; S3. Put the viscous complex solution prepared in S2, fibroin, tannic acid, and dimethyl sulfoxide into a reaction kettle with a power of 2.4 kW, react at a temperature of 40°C for 5 h, at a reaction speed of 400 r / min, and obtain a gel substrate after cross-linking reaction; S4. Put apocynum venetum, schisandra chinensis, and notoginseng into a crusher with a power of 1.7 kW, crush at a temperature of 26°C for 18 min, at a crushing speed of 4000 r / min, and after crushing, put them into an ultrasonic extractor with a power of 1.4 kW together with 70% ethanol solution, with an extraction pressure of 1.5 MPa, an extraction frequency of 25 kHz, and an extraction temperature of 43°C for 11 min for extraction to obtain a blood circulation-promoting extract; S5. Put the blood circulation-promoting extract prepared in S4, eicosapentaenoic acid, and boswellic acid into the vasodilating solution prepared in S1, stir at a temperature of 31°C, a stirring speed of 310 r / min, and a stirring time of 18 min, and mix evenly to obtain a blood circulation-promoting and pain-relieving solution; S6. Add the gel substrate prepared in S3 to the blood circulation-promoting and pain-relieving solution prepared in S5, stir at a temperature of 28°C, a stirring speed of 310 r / min, and a stirring time of 26 min, and fully adsorb to obtain the blood circulation-promoting and pain-relieving micelles.

[0037] In S1, the mass fraction of the succinimide in a 50% ethanol solution is 4%. In S2, the mass fraction of the aqueous polyurethane in ultrapure water is 26%.

[0038] In S3, the mass fraction of the fibroin in dimethyl sulfoxide is 10%. In S4, the material-liquid ratio of the pseudo-ginseng and a 70% ethanol solution is 1:3 g / mL.

[0039] The preparation method of the penetration-promoting and fatigue-relieving particles specifically comprises the following steps: L1. Put sodium chenodeoxycholate, polyacrylamide, and ultrapure water into a constant-temperature stirrer with a power of 1.7 kW. The constant-temperature stirring temperature is 35°C, the constant-temperature stirring speed is 400 r / min, and the constant-temperature stirring duration is 5 h for reaction to form a complex solution. L2. Put parsley, spinach, and ultrapure water into a grinder with a power of 2.2 kW. The grinding temperature is 88°C, the grinding duration is 36 min, and the grinding speed is 19000 r / min for grinding. Filter with a sterile filter cloth, and put it together with N-acetyl-L-carnitine, salidroside, and theanine into a stirrer with a power of 1.7 kW. The stirring temperature is 31°C, the stirring speed is 310 r / min, and the stirring duration is 13 min to obtain a fatigue-relieving solution. L3. Put the fatigue-relieving solution prepared in L2 into the complex solution prepared in L1. The constant-temperature stirring temperature is 26°C, the constant-temperature stirring speed is 400 r / min, and the constant-temperature stirring duration is 25 min. After mixing evenly, put it into a vacuum freeze nano spray dryer with a power of 2.6 kW. The vacuum degree is -0.05 MPa, the freeze nano spray drying temperature is -40°C, the freeze nano spray drying particle size is 100 nm, and the freeze nano spray drying duration is 2.8 h. After freeze spray drying, penetration-promoting and fatigue-relieving particles are obtained.

[0040] In L1, the mass fraction of the sodium chenodeoxycholate in ultrapure water is 17%, and the mass fraction of the polyacrylamide in ultrapure water is 19%.

[0041] In L2, the material-liquid ratio of the parsley and ultrapure water is 5:1.5 g / mL.

[0042] This embodiment also provides a preparation method of a blood circulation-promoting and fatigue-relieving plaster, which specifically comprises the following steps: Step 1. Put anhydrous betaine, the extract of Cornus officinalis, bisabolol, and a 50% ethanol solution into a stirrer with a power of 1.7 kW. The stirring temperature is 33°C, the stirring speed is 500 r / min, and the stirring duration is 14 min for sufficient dissolution to obtain a soothing and anti-allergic agent. Step 2: Put the blood circulation-promoting and pain-relieving micelles into the soothing and anti-allergic agent prepared in Step 1. Stir at a temperature of 33°C, a rotation speed of 500 r / min, and a stirring duration of 26 min. After sufficient adsorption, a gel substrate is obtained. Step 3: Put the penetration-enhancing and fatigue-relieving particles into the gel substrate prepared in Step 2. Use an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring rotation speed of 20,000 r / min, a stirring temperature of 25°C, and a stirring duration of 10 min. After uniform dispersion, a blood circulation-promoting and fatigue-relieving mixture is obtained. Step 4: Coat the blood circulation-promoting and fatigue-relieving mixture prepared in Step 3 on a medical adhesive tape with a coating thickness of 1.2 mm to obtain a blood circulation-promoting and fatigue-relieving plaster.

[0043] In Step 1, the mass fraction of anhydrous betaine in the 50% ethanol solution is 2.3%.

[0044] Example 3: This example provides a blood circulation-promoting and fatigue-relieving plaster and its preparation method. The blood circulation-promoting and fatigue-relieving plaster contains the following components in parts by weight: 55 parts of blood circulation-promoting and pain-relieving micelles, 28 parts of penetration-enhancing and fatigue-relieving particles, 2.2 parts of anhydrous betaine, 4.1 parts of cornel extract, and 2 parts of bisabolol.

[0045] The raw materials for preparing the blood circulation-promoting and pain-relieving micelles include the following components in parts by weight: 14 parts of succinimide, 15 parts of 2,6-dimethylpyridine, 10 parts of waterborne polyurethane, 13 parts of polyvinyl alcohol, 14 parts of fibroin, 1.4 parts of tannic acid, 7 parts of apocynum venetum, 9 parts of schisandra chinensis, 10 parts of notoginseng, 2.7 parts of eicosapentaenoic acid, and 2.9 parts of boswellic acid; The raw materials for preparing the penetration-enhancing and fatigue-relieving particles include the following components in parts by weight: 9 parts of chenodeoxycholic acid sodium, 13 parts of polyacrylamide, 10 parts of parsley, 16 parts of spinach, 2.5 parts of N-acetyl-L-carnitine, 1.8 parts of salidroside, and 1.7 parts of theanine.

[0046] The preparation method of the blood circulation-promoting and pain-relieving micelles specifically includes the following steps: S1: Put succinimide, 2,6-dimethylpyridine, and 50% ethanol solution into a stirrer with a power of 1.8 kW. Stir at a temperature of 33°C, a rotation speed of 320 r / min, and a stirring duration of 15 min for mixing and dissolution to obtain a vasodilating solution; S2: Put waterborne polyurethane, polyvinyl alcohol, and ultrapure water into a constant-temperature stirrer with a power of 2.6 kW. Stir at a constant temperature of 90°C for a constant-temperature stirring duration of 4.5 h and a constant-temperature stirring rotation speed of 1000 r / min for stirring reaction to form a viscous complex solution; S3. Put the viscous composite solution prepared in S2, silk fibroin, tannic acid, and dimethyl sulfoxide into a reaction kettle with a power of 2.6 kW, a reaction temperature of 40 °C, a reaction duration of 6 h, and a reaction rotation speed of 500 r / min. After cross-linking reaction, a gel substrate is obtained; S4. Put Apocynum venetum, Schisandra chinensis, and Panax notoginseng into a crusher with a power of 1.8 kW, a crushing temperature of 28 °C, a crushing duration of 20 min, and a crushing rotation speed of 4500 r / min. After crushing, put them into an ultrasonic extractor with a power of 1.6 kW together with 70% ethanol solution. The extraction pressure is 1.5 MPa, the extraction frequency is 25 kHz, the extraction temperature is 45 °C, and the extraction duration is 13 min for extraction to obtain a circulation-promoting extract; S5. Put the circulation-promoting extract prepared in S4, eicosapentaenoic acid, and boswellic acid into the vasodilating solution prepared in S1. The stirring temperature is 33 °C, the stirring rotation speed is 320 r / min, and the stirring duration is 20 min to mix evenly to obtain a circulation-promoting and pain-relieving solution; S6. Add the gel substrate prepared in S3 to the circulation-promoting and pain-relieving solution prepared in S5. The stirring temperature is 33 °C, the stirring rotation speed is 320 r / min, and the stirring duration is 30 min to fully adsorb to obtain a circulation-promoting and pain-relieving micelle.

[0047] In S1, the mass fraction of succinimide in 50% ethanol solution is 5%. In S2, the mass fraction of aqueous polyurethane in ultrapure water is 30%.

[0048] In S3, the mass fraction of silk fibroin in dimethyl sulfoxide is 10%. In S4, the material-liquid ratio of Panax notoginseng to 70% ethanol solution is 1:3 g / mL.

[0049] The preparation method of the penetration-promoting and fatigue-relieving particles specifically includes the following steps: L1. Put sodium chenodeoxycholate, polyacrylamide, and ultrapure water into a constant-temperature stirrer with a power of 1.9 kW, a constant-temperature stirring temperature of 40 °C, a constant-temperature stirring rotation speed of 500 r / min, and a constant-temperature stirring duration of 6 h for reaction to form a composite solution; L2. Put parsley, spinach, and ultrapure water into a grinder with a power of 2.4 kW, a grinding temperature of 90 °C, a grinding duration of 40 min, and a grinding rotation speed of 20000 r / min for grinding. Filter with a sterile filter cloth and put it into a stirrer with a power of 1.8 kW together with N-acetyl-L-carnitine, salidroside, and theanine. The stirring temperature is 33 °C, the stirring rotation speed is 320 r / min, and the stirring duration is 15 min to obtain a fatigue-relieving solution; L3. Put the anti-fatigue solution prepared in L2 into the composite solution prepared in L1. The constant-temperature stirring temperature is 30 °C, the constant-temperature stirring speed is 500 r / min, and the constant-temperature stirring duration is 30 min. After mixing evenly, put it into a vacuum freeze nano spray dryer with a power of 2.8 kW, a vacuum degree of -0.05 MPa, a freeze nano spray drying temperature of -40 °C, a freeze nano spray drying particle size of 100 nm, and a freeze nano spray drying duration of 3 h. After freeze spray drying, pro-penetration anti-fatigue particles are obtained.

[0050] In L1, the mass fraction of chenodeoxycholic acid sodium in ultrapure water is 15 - 18%, and the mass fraction of polyacrylamide in ultrapure water is 21%.

[0051] In L2, the material-liquid ratio of parsley to ultrapure water is 5:1.5 g / mL.

[0052] This embodiment also provides a preparation method of a promoting blood circulation and anti-fatigue plaster, which specifically includes the following steps: Step 1. Put anhydrous betaine, cornus officinalis extract, bisabolol, and 50% ethanol solution into a stirrer with a power of 1.8 kW. The stirring temperature is 35 °C, the stirring speed is 600 r / min, and the stirring duration is 15 min. After fully dissolving, a soothing and anti-allergic agent is obtained. Step 2. Put the promoting blood circulation and pain-relieving micelle into the soothing and anti-allergic agent prepared in Step 1. The stirring temperature is 36 °C, the stirring speed is 600 r / min, and the stirring duration is 30 min. After full adsorption, a gel substrate is obtained. Step 3. Put the pro-penetration anti-fatigue particles into the gel substrate prepared in Step 2. Use an ultrasonic stirrer with a power of 1.8 kW, an ultrasonic frequency of 15 kHz, a stirring speed of 20000 r / min, a stirring temperature of 25 °C, and a stirring duration of 10 min. After uniform dispersion, a promoting blood circulation and anti-fatigue mixture is obtained. Step 4. Coat the promoting blood circulation and anti-fatigue mixture prepared in Step 3 on a medical adhesive tape with a coating thickness of 1.3 mm to obtain a promoting blood circulation and anti-fatigue plaster.

[0053] In Step 1, the mass fraction of anhydrous betaine in 50% ethanol solution is 2.3%.

[0054] Comparative Example 1: This comparative example provides a plaster patch and its preparation method. The difference from Example 1 is only that the added promoting blood circulation and pain-relieving micelle does not contain a vasodilating solution, and the other components, component contents, and method steps are the same as those in Example 1.

[0055] Comparative Example 2: This comparative example provides a plaster patch and a preparation method thereof. The difference from Example 1 is only that the penetration-enhancing and fatigue-relieving particles added do not contain the complex solution, and the other components, component contents, and method steps are the same as those in Example 1.

[0056] Experimental Example 1: Determination test for significant effect on promoting blood circulation and relieving fatigue.

[0057] The steps for the determination test of the effect of promoting blood circulation and relieving fatigue of the plaster for promoting blood circulation and relieving fatigue prepared in Examples 1-3 of the present invention are as follows: (1) Select 60 SPF-grade mice at 8 weeks of age and weighing 25 g (purchased from Chengdu Dashuo Animal Co., Ltd.). Divide them into 6 groups with 10 mice in each group randomly. Cage the mice separately, keep the cages clean and dry, and feed them at a temperature of 24°C and a humidity of 50% with free access to food and water. (2) After anesthetizing the mice in step (1), use a laser Doppler microcirculation analyzer to measure the microcirculation blood flow volume (PU) of the mouse auricle, and record it as the initial microcirculation blood flow volume of the mouse auricle. (3) Cut the plasters for promoting blood circulation and relieving fatigue prepared in Examples 1-3 into small round pieces with a diameter of 8 mm, and stick them on the auricles of the mice in groups of Examples 1-3 respectively. Cut the plaster patches prepared in Comparative Examples 1-2 into small round pieces with a diameter of 8 mm, and stick them on the auricles of the mice in groups of Comparative Examples 1-2 respectively. Cut the commercially available ordinary blood-activating plaster (purchased from Henan Jushitang Pharmaceutical Co., Ltd.) into small round pieces with a diameter of 8 mm, and stick it on the auricles of the control group mice. Measure the blood flow volume of the mice 20 minutes after sticking, record the data, and calculate the difference in microcirculation blood flow volume of the mouse auricle according to the formula. The difference in microcirculation blood flow volume of the mouse auricle = the microcirculation blood flow volume of the mouse auricle after sticking - the initial microcirculation blood flow volume of the mouse auricle. (4) Select 60 SPF-grade mice at 8 weeks of age and weighing 25 g (purchased from Chengdu Dashuo Animal Co., Ltd.). Divide them into 6 groups with 10 mice in each group randomly. Cage the mice separately, keep the cages clean and dry, and feed them at a temperature of 24°C and a humidity of 50% with free access to food and water. (5) Place the mice in step (4) on a roller with a rotation speed of 18 r / min to make the mice start to pedal the wheel, and record the number of times the mice fall off the wheel due to muscle fatigue within 20 minutes, which is recorded as the number of wheel falls before sticking. (6) On the 2nd day, the blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3 were cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in Groups 1-3 of Examples respectively. The plaster patches prepared in Comparative Examples 1-2 were cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in Groups 1-2 of Comparative Examples respectively. The commercially available ordinary blood-activating plaster (purchased from Henan Jushitang Pharmaceutical Co., Ltd.) was cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in the control group. 30 minutes after pasting, the mice in each group were placed on a roller with a rotation speed of 18 r / min to make the mice start to pedal the wheel, and the number of times the mice fell off the wheel due to muscle fatigue within 20 minutes was recorded and recorded as the number of times of falling off the wheel after pasting.

[0058] Result analysis: Figure 1 This is the scanning electron micrograph of the internal structure of the gel substrate described in the present invention. Figure 2 This is the result graph of the difference in microcirculation blood flow volume of the auricles of the mice described in Experimental Example 1 of the present invention. As shown in the figure, after the blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3 were cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in Groups 1-3 of Examples for 20 minutes, the differences in microcirculation PU of the auricles of the mice in Groups 1-3 of Examples were 40.5, 41.43, and 43.5 respectively. After the plaster patches prepared in Comparative Examples 1-2 were cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in Groups 1-2 of Comparative Examples for 20 minutes, the differences in microcirculation PU of the auricles of the mice in Groups 1-2 of Comparative Examples were 21.37 and 33.23 respectively. After the commercially available ordinary blood-activating plaster (purchased from Henan Jushitang Pharmaceutical Co., Ltd.) was cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in the control group for 20 minutes, the difference in microcirculation PU of the auricles of the mice in the control group was 15.07. Figure 3 This is the result graph of the number of times of falling off the wheel of the mice described in Experimental Example 1 of the present invention. As shown in the figure, after the mice in Groups 1-3 of Examples used the blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3 for 30 minutes and started to pedal the wheel on a roller with a rotation speed of 18 r / min, the number of times of falling within 20 minutes decreased significantly. After the mice in the control group used the commercially available ordinary blood-activating plaster for 30 minutes and started to pedal the wheel on a roller with a rotation speed of 18 r / min, the difference in the number of times of falling within 20 minutes before and after comparison was basically small. It shows that the blood circulation-promoting and fatigue-relieving plaster prepared by the present invention has the effects of promoting blood circulation and anti-fatigue on mice, and the effect is remarkable.

[0059] Experimental Example 2: Determination test of stable and lasting efficacy.

[0060] The steps of the efficacy stability determination test of the blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3 of the present invention are as follows: (1) Under the conditions of a temperature of 36 °C and a humidity of 85%, the blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3, the plaster patches prepared in Comparative Examples 1-2, and the commercially available ordinary activating blood circulation plasters prepared in the control group (purchased from Henan Jushitang Pharmaceutical Co., Ltd.) were stored for 48 d, and then the blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3 after storage, the plaster patches prepared in Comparative Examples 1-2 after storage, and the commercially available ordinary activating blood circulation plasters prepared in the control group after storage were obtained; (2) Sixty 8-week-old SPF mice with a body weight of 25 g (purchased from Chengdu Dashuo Animal Co., Ltd.) were selected. They were divided into 6 groups with 10 mice in each group and randomly. The mice were caged separately, and the cages were kept clean and dry. They were fed at a temperature of 24 °C and a humidity of 50% with free access to food and water; (2) After anesthetizing the mice in step (1), the microcirculation blood flow volume (PU) of the auricles of the mice was measured with a laser Doppler microcirculation analyzer, and the initial microcirculation blood flow volume of the auricles of the mice was recorded; (3) The blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3 after storage were cut into small round pieces with a diameter of 8 mm and respectively pasted on the auricles of the mice in groups 1-3 of Example 1. The plaster patches prepared in Comparative Examples 1-2 after storage were cut into small round pieces with a diameter of 8 mm and respectively pasted on the auricles of the mice in groups 1-2 of Comparative Example 1. The commercially available ordinary activating blood circulation plasters (purchased from Henan Jushitang Pharmaceutical Co., Ltd.) after storage were cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in the control group. Twenty minutes after pasting, the blood flow volume of the mice was measured, the data were recorded, and the difference in the microcirculation blood flow volume of the auricles of the mice was calculated according to the formula. The difference in the microcirculation blood flow volume of the auricles of the mice after storage = the microcirculation blood flow volume of the auricles of the mice after pasting - the initial microcirculation blood flow volume of the auricles of the mice; (4) Sixty 8-week-old SPF mice with a body weight of 25 g (purchased from Chengdu Dashuo Animal Co., Ltd.) were selected. They were divided into 6 groups with 10 mice in each group and randomly. The mice were caged separately, and the cages were kept clean and dry. They were fed at a temperature of 24 °C and a humidity of 50% with free access to food and water; (5) The mice in step (4) were placed on a roller with a rotation speed of 18 r / min to make the mice start to pedal the wheel, and the number of times the mice fell off the wheel due to muscle fatigue within 20 min was recorded and recorded as the number of wheel falls before pasting; (6)On the 2nd day, the promoting blood circulation and relieving fatigue plasters prepared in Examples 1-3 after storage were cut into small round pieces with a diameter of 8 mm, and were respectively pasted on the auricles of the mice in Groups 1-3 of Example 1-3. The plaster patches prepared in Comparative Examples 1-2 after storage were cut into small round pieces with a diameter of 8 mm, and were respectively pasted on the auricles of the mice in Groups 1-2 of Comparative Examples 1-2. The commercially available ordinary blood-activating plaster (purchased from Henan Jushitang Pharmaceutical Co., Ltd.) after storage was cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in the control group. 30 minutes after pasting, the mice in each group were placed on a roller with a rotation speed of 18 r / min to make the mice start to pedal the wheel, and the number of times the mice fell off the wheel due to muscle fatigue within 20 minutes was recorded, which was recorded as the number of times of falling off the wheel after pasting after storage.

[0061] Result analysis: Figure 4 This is the result graph of the difference in microcirculation blood flow in the auricles of mice after storage described in Experimental Example 2 of the present invention. As shown in the figure, after the promoting blood circulation and relieving fatigue plasters prepared in Examples 1-3 after storage were cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in Groups 1-3 of Example 1-3 for 20 minutes, the differences in microcirculation PU in the auricles of the mice in Groups 1-3 of Example 1-3 were 37.65, 38.60, and 40.81 respectively. After the plaster patches prepared in Comparative Examples 1-2 after storage were cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in Groups 1-2 of Comparative Examples 1-2 for 20 minutes, the differences in microcirculation PU in the auricles of the mice in Groups 1-2 of Comparative Examples 1-2 were 17.88 and 29.45 respectively. After the commercially available ordinary blood-activating plaster after storage was cut into small round pieces with a diameter of 8 mm and pasted on the auricles of the mice in the control group for 20 minutes, the difference in microcirculation PU in the auricles of the mice in the control group was 3.31; Figure 5 This is the result graph of the number of times of falling off the wheel of mice after storage described in Experimental Example 2 of the present invention. As shown in the figure, after 30 minutes of using the promoting blood circulation and relieving fatigue plasters prepared in Examples 1-3 after storage by the mice in Groups 1-3 of Example 1-3, they started to pedal the wheel on a roller with a rotation speed of 18 r / min, and the number of times of falling within 20 minutes still decreased significantly. After 30 minutes of using the commercially available ordinary blood-activating plaster by the mice in the control group after storage, they started to pedal the wheel on a roller with a rotation speed of 18 r / min, and the difference in the number of times of falling within 20 minutes before and after comparison was basically small. This shows that the promoting blood circulation and relieving fatigue plaster prepared by the present invention has a significant effect on promoting blood circulation and relieving fatigue, and the drug effect is stable and lasting after long-term use and storage.

[0062] Experimental Example 3: Determination test on non-irritation to skin after long-term use.

[0063] The steps for the determination test on the irritation of the promoting blood circulation and relieving fatigue plaster prepared in Examples 1-3 of the present invention to the skin after long-term use are as follows: (1)Select 60 healthy white male guinea pigs with a body weight of 700 g, 10 in each group, and randomly divide them into 6 groups; (2)Routinely depilate the two sides of the back of white guinea pigs. The depilation area is 3 cm × 3 cm. After 24 h of depilation, observe that the local skin is intact without damage. In the experiment, the left and right sides of the same guinea pig are compared with each other. On the left side of the back of the guinea pigs in Example Groups 1-3, apply the blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3 respectively. On the left side of the back of the guinea pigs in Comparative Example Groups 1-2, apply the plaster patches prepared in Comparative Examples 1-2 respectively. On the left side of the back of the guinea pigs in the control group, apply the commercially available ordinary promoting blood circulation plaster (purchased from Henan Jushitang Pharmaceutical Co., Ltd.). After 24 h of application, observe the adhesion conditions of the blood circulation-promoting and fatigue-relieving plasters prepared in Examples 1-3, the plaster patches prepared in Comparative Examples 1-2, and the commercially available ordinary promoting blood circulation plaster on the back of the guinea pigs, and observe the treated parts after cleaning with warm water; (3)At 24, 48, and 72 h after cleaning with warm water, observe and record with the naked eye whether there are erythema and edema on the left side of the back of the guinea pigs. Calculate the average score of each group of guinea pigs at each observation time point according to Table 1, and evaluate the stimulation intensity according to Table 2, and record the results.

[0064] Table 1. Scoring criteria for skin irritation reaction

[0065] Table 2. Evaluation criteria for skin irritation intensity

[0066] Table 3. Table of skin irritation reaction results

[0067] Result analysis: Figure 6 This is the finished product diagram of the blood circulation-promoting and fatigue-relieving plaster of the present invention. As shown in Figure 6 and Table 3, the skin physiological structure of guinea pigs is similar to that of humans. No erythema or edema appeared on the back of the white guinea pigs in Example Groups 1-3, while mild irritation appeared on the back of the white guinea pigs in the control group, and the commercially available ordinary promoting blood circulation plaster in the control group fell off on the back of the white guinea pigs. This shows that the blood circulation-promoting and fatigue-relieving plaster prepared by the present invention has excellent biocompatibility, is non-toxic and non-irritating to the skin, and has strong adhesion ability.

[0068] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

[0069] The above description of the present invention and its embodiments is not restrictive. What is shown in the drawings is only one of the embodiments of the present invention, and the actual application is not limited thereto. In general, if those of ordinary skill in the art are inspired by it and, without departing from the spirit of the present invention, design in a non-creative manner similar ways and embodiments to this technical solution, they shall fall within the protection scope of the present invention.

Claims

1. A circulation-promoting and fatigue-relieving plaster, characterized in that: The raw materials for preparing the circulation-promoting fatigue-relieving plaster specifically include the following components in parts by weight: 45-55 parts of circulation-promoting analgesic micelles, 22-28 parts of penetration-promoting fatigue-relieving particles, 1.8-2.2 parts of anhydrous betaine, 3.4-4.1 parts of cornus officinalis extract, and 1.5-2 parts of bisabolol; The raw materials for preparing the circulation-promoting and analgesic micelles include the following components in parts by weight: 11-14 parts of succinimide, 12-15 parts of 2,6-dimethylpyridine, 8-10 parts of waterborne polyurethane, 11-13 parts of polyvinyl alcohol, 9-14 parts of silk fibroin, 1.2-1.4 parts of tannic acid, 4-7 parts of apocynum venetum, 8-9 parts of schisandra chinensis, 7-10 parts of notoginseng, 2.3-2.7 parts of eicosapentaenoic acid, and 2.6-2.9 parts of boswellic acid; The raw materials for preparing the penetration-promoting fatigue-relieving particles include the following components in parts by weight: 6-9 parts of sodium chenodeoxycholate, 11-13 parts of polyacrylamide, 8-10 parts of parsley, 12-16 parts of spinach, 2-2.5 parts of N-acetyl-L-carnitine, 1.5-1.8 parts of salidroside, and 1.4-1.7 parts of theanine.

2. The circulation-promoting fatigue-relieving plaster according to claim 1, characterized in that: The preparation method of the circulation-promoting analgesic micelle specifically comprises the following steps: S1. Mix and dissolve succinimide, 2,6-lutidine, and 50% ethanol solution to obtain a vasodilator solution; S2, stirring and reacting waterborne polyurethane with polyvinyl alcohol and ultrapure water to form a viscous composite solution; S3, cross-linking the viscous complex solution prepared in S2 with silk fibroin, tannic acid, and dimethyl sulfoxide to obtain a gel substrate; S4, crushing Apocynum venetum, Schisandra chinensis, and Panax notoginseng, and extracting them with 70% ethanol solution to obtain a circulation-promoting extract; S5, adding the circulation-promoting extract, eicosapentaenoic acid and boswellic acid prepared in S4 into the vasodilating solution prepared in S1, and mixing well to obtain a circulation-promoting analgesic solution; S6. Add the gel base material prepared in S3 to the circulation-promoting and analgesic solution prepared in S5, and fully adsorb to obtain the circulation-promoting and analgesic micelles.

3. The circulation-promoting fatigue-relieving plaster according to claim 2, characterized in that: The preparation method of the penetration-promoting fatigue-relieving particles specifically comprises the following steps: L1, stirring sodium chenodeoxycholate, polyacrylamide and ultrapure water to react to form a complex solution; L2. Mix parsley, spinach and ultrapure water, grind them into a pulp, filter them with a sterile filter cloth, and mix them with N-acetyl-L-carnitine, salidroside and theanine to obtain a fatigue-relieving solution; L3. Add the fatigue-relieving solution prepared in L2 into the complex solution prepared in L1, stir and mix, and freeze-spray dry to obtain penetration-promoting fatigue-relieving particles.

4. A method for preparing the circulation-promoting fatigue-relieving plaster according to any one of claims 1 to 3, characterized in that: The specific steps include: Step 1: fully dissolve anhydrous betaine, cornus officinalis extract, bisabolol, and 50% ethanol solution to obtain a soothing anti-allergic agent; Step 2, placing the circulation-promoting analgesic micelle into the soothing anti-allergic agent prepared in step 1, and obtaining a gel matrix after sufficient adsorption; Step 3, uniformly dispersing the penetration-promoting fatigue-relieving particles in the gel matrix prepared in step 2 to obtain a circulation-promoting fatigue-relieving mixture; Step 4: Apply the circulation-promoting and fatigue-relieving mixture prepared in step 3 on a medical adhesive tape with a coating thickness of 1-1.3 mm to obtain a circulation-promoting and fatigue-relieving plaster.

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