Device and method for extracting selenoprotein from selenium-rich green tea

Abstract

The invention discloses a selenium-rich green tea selenoprotein extraction device and method, and relates to the technical field of selenoprotein extraction.The selenium-rich green tea selenoprotein extraction device comprises a driving motor and a driving rod connected to the output end of the driving motor and further comprises a top base, a collecting groove and a clamping groove are formed in the lower surface of the top base, and one end of the driving rod is connected with a driving assembly; the exterior of the driving assembly is connected with a stirring rod and a fixing cylinder, and the driving rod is arranged in the stirring rod. According to the selenium-rich green tea selenoprotein extraction device and method, the release assembly is arranged to be matched with the driving assembly to stir mixed liquid, the driving assembly can drive the trigger assembly to stretch out and draw back when moving, and when the trigger assembly stretches to the longest, the locking assembly is triggered to lock the trigger assembly, so that the selenium protein is extracted, and the extraction efficiency is improved. And the release assembly is synchronously driven to release the connection between the driving assembly and the stirring rod so as to stop stirring of the mixed liquid, so that the mixed liquid is subjected to centrifugal treatment.

Description

Technical Field

[0001] The present invention relates to the technical field of selenium protein extraction, and particularly relates to an extraction device and an extraction method for selenium protein in selenium-rich green tea. Background Art

[0002] Selenium proteins are a class of proteins containing selenocysteine residues, and they are key biomolecules for trace element selenium to achieve physiological functions. Selenium proteins play important biological functions in the human body, including antioxidant, immunomodulatory, and toxin antagonistic effects.

[0003] Selenium proteins in tea account for an important proportion in terms of content. The existence of these selenium proteins makes tea an economical and excellent selenium supplement source. Most of the selenium proteins in tea are alkali-soluble and are not easily dissolved out by water soaking, and the proportion remaining in tea dregs is as high as 23.18%. Therefore, extracting selenium protein from tea by an extraction device is the current common method.

[0004] In the prior art, when extracting selenium protein from tea, various devices are required for different treatments during extraction, and the raw materials need to be continuously transferred from one device to another during different steps. When the raw materials are in an aqueous solution state, this transfer is extremely likely to cause solution pollution or excessive solution residue and waste. Therefore, it is necessary to minimize the transfer of the solution and make the extraction integrated and continuously processed on one device. Summary of the Invention

[0005] The purpose of the present invention is to provide an extraction device and an extraction method for selenium protein in selenium-rich green tea to solve the above deficiencies in the prior art.

[0006] To achieve the above purpose, the present invention provides the following technical solutions:

[0007] An extraction device for selenium protein in selenium-rich green tea includes a driving motor and a driving rod connected to the output end of the driving motor, and further includes a top seat. A converging groove and a clamping groove are provided on the lower surface of the top seat. One end of the driving rod is connected to a driving component, and a stirring rod and a fixed cylinder are connected to the outside of the driving component. The driving rod is arranged inside the stirring rod.

[0008] A locking component is arranged in the converging groove, a triggering component is arranged in the fixed cylinder, a releasing component is arranged inside the stirring rod, a limiting groove is provided at the lower end of the fixed cylinder, and the releasing component passes through the limiting groove and is connected to the triggering component. When the driving component moves, it drives the triggering component to expand and contract. When the triggering component extends to the longest, it triggers the locking component to lock the triggering component, and simultaneously drives the releasing component to disconnect the connection between the driving component and the stirring rod.

[0009] As a further preferred solution in the embodiments of the present invention, the driving assembly includes a first screw rod connected to the upper end of the driving rod, and one end of the first screw rod is sleeved with a second screw rod. A first slot is provided on the surface of the first screw rod, a protruding slot is provided inside the second screw rod, the protruding slot is slidably connected to the first slot, and a second slot is provided on the surface of the second screw rod.

[0010] As a further preferred solution in the embodiments of the present invention, the triggering assembly includes a multi-stage telescopic cylinder sleeved inside a fixed cylinder. A first inner slot is provided on the inner surface of the fixed cylinder. A first sliding strip is fixedly connected to the surface of the multi-stage telescopic cylinder. The first sliding strip is slidably matched with the first inner slot. A threaded hole is provided at the lower end of the multi-stage telescopic cylinder. A second inner slot is provided on the inner surface of the multi-stage telescopic cylinder. A locking cylinder is sleeved at the upper end of the multi-stage telescopic cylinder. A second sliding strip is fixedly connected to the surface of the locking cylinder. The second sliding strip is slidably matched with the second inner slot. The lower end of the second screw rod is rotationally matched with the threaded hole.

[0011] As a further preferred solution in the embodiments of the present invention, the locking assembly includes a top contact seat slidably sleeved on the surface of the converging tube. A return spring is connected between the top contact seat and the upper surface inside the converging groove. A first locking seat is connected to the surface of the top contact seat. One end of the first locking seat is rotatably connected to a locking rod. One end of the locking rod is rotatably connected to a second locking seat. One end of the second locking seat is connected to a locking claw.

[0012] As a further preferred solution in the embodiments of the present invention, a top contact groove is provided at the upper end of the locking cylinder. A locking groove is provided on the side surface of the locking cylinder. A converging tube is fixedly connected inside the converging groove. The return spring is provided on the surface of the converging tube. The converging tube is slidably matched with the top contact groove. The locking claw is located in the clamping groove and is rotatably matched with the two side surfaces of the clamping groove. The locking claw is clamped and matched with the locking groove.

[0013] As a further preferred solution in the embodiments of the present invention, stirring blades are fixedly connected to the surface of the stirring rod. A driving groove is provided inside the stirring rod. The releasing assembly includes a first connecting seat connected to the surface of the first screw rod. A connecting strip is rotatably connected to the lower end of the first connecting seat. A second connecting seat is rotatably connected to the lower end of the connecting strip. A driving sliding strip is slidably connected in the driving groove. One end of the second connecting seat is fixedly connected to one side surface of the driving sliding strip.

[0014] As a further preferred solution in the embodiments of the present invention, it further includes a base. A support rod is connected to the upper surface of the base by bolts. The upper end of the support rod is fixedly connected to a base. The base is provided with a through hole. The driving motor is arranged on the upper surface of the base. The output end of the driving motor passes through the through hole and is connected to the lower end of the driving rod.

[0015] As a further preferred solution in the embodiments of the present invention, an outer shell is rotatably connected to the upper surface of the base, the upper end of the outer shell is fixedly connected to the top seat, a feed pipe is communicated with the upper surface of the top seat, and an inner shell, a filter frame and a mask expansion plate are sequentially arranged in the outer shell from outside to inside. The inner shell and the filter frame are rotatably connected to the base, and the upper end of the mask expansion plate is fixedly connected to the lower surface of the top seat.

[0016] As a further preferred solution in the embodiments of the present invention, a heating coil is arranged between the outer shell and the inner shell, a circuit pipe is arranged on the surface of the heating coil, a heater is arranged on the upper surface of the base, and one end of the circuit pipe penetrates through the outer shell and is connected to the heater.

[0017] A method for extracting selenium-rich green tea selenium protein is extracted by the above-mentioned extraction device for selenium-rich green tea selenium protein, and comprises the following steps:

[0018] S1. Feeding: After adding tea powder into the device through the feed pipe, add 0.1 mol / L sodium hydroxide solution, and ensure that the material-liquid ratio of the tea powder to the solution is 1:60 g / mL;

[0019] S2. Stirring extraction: After starting the heater to heat the temperature of the mixed solution to 69 °C, start the driving motor to stir the mixed solution for 3 h;

[0020] S3. Centrifugal treatment: After stirring, perform centrifugal treatment on the mixed solution for 10 min, and the centrifugal rate is 3600 r / min;

[0021] S4. Adjusting pH: After centrifugation, add acid solution through the feed pipe to adjust the pH to 2.0, and let it stand for a period of time to precipitate proteins;

[0022] S5. Secondary centrifugation: Centrifuge the mixed solution again for 10 min, and the centrifugal rate is 3600 r / min;

[0023] S6. Completion of extraction: Suck out the upper clear liquid and discard it, take out the lower solid mass and put it into a vacuum dryer for drying to obtain a selenium protein sample.

[0024] In the above technical solution, the beneficial effects of the extraction device and extraction method for selenium-rich green tea selenium protein provided by the present invention are as follows:

[0025] In the present invention, a release component is arranged to cooperate with a driving component to stir the mixed liquid, and when the driving component moves, it will also drive a trigger component to expand and contract. When the trigger component extends to its longest length, a locking component is triggered to lock the trigger component, and at the same time, the release component is driven to release the connection between the driving component and the stirring rod, so as to stop stirring the mixed liquid, thereby performing centrifugal treatment on the mixed liquid, enabling the device to automatically switch to the centrifugal state after passive stirring, and performing stirring and then centrifugation on the mixed liquid without transfer and automatically.

[0026] It should be understood that the foregoing general description and the following detailed description are merely exemplary and explanatory and are not intended to limit the present disclosure.

[0027] This application document provides an overview of various implementations or examples of the technology described in the present disclosure, and is not a full disclosure of the entire scope or all features of the disclosed technology. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the following will briefly introduce the drawings required to be used in the embodiments. Obviously, the drawings in the following description are only some embodiments recorded in the present invention, and those of ordinary skill in the art can also obtain other drawings based on these drawings.

[0029] Figure 1 Schematic diagram of the overall structure provided by an embodiment of the present invention;

[0030] Figure 2 Exploded view of the housing provided by an embodiment of the present invention;

[0031] Figure 3 Schematic diagram of the internal structure of the housing provided by an embodiment of the present invention;

[0032] Figure 4 Schematic diagram of the structure of the fixed cylinder and the stirring rod provided by an embodiment of the present invention;

[0033] Figure 5 Provided by an embodiment of the present invention Figure 4 Enlarged schematic diagram of part A;

[0034] Figure 6 Schematic diagram of the internal structure of the fixed cylinder provided by an embodiment of the present invention;

[0035] Figure 7 Provided by an embodiment of the present invention Figure 6 Enlarged schematic diagram of part B;

[0036] Figure 8 Schematic diagram of the internal structure of the stirring rod provided by an embodiment of the present invention;

[0037] Figure 9 Internal structure schematic diagram of the top seat provided by the embodiment of the present invention.

[0038] Explanation of reference numerals:

[0039] 1. Base; 101. Support rod; 102. Base; 103. Driving motor; 2. Heater; 201. Circuit tube; 202. Heating coil; 3. Outer shell; 301. Top seat; 302. Converging groove; 303. Converging tube; 304. Clamping groove; 305. Feed pipe; 306. Inner shell; 307. Filter frame; 308. Mask expansion plate; 4. Driving rod; 401. First screw rod; 402. First slot; 403. Second screw rod; 404. Second slot; 5. Fixed cylinder; 501. Fixed strip; 502. First inner slot; 503. Multistage telescopic cylinder; 504. First sliding strip; 505. Threaded hole; 506. Second inner slot; 507. Locking cylinder; 508. Second sliding strip; 509. Locking groove; 510. Top contact groove; 511. Connecting rod; 6. Stirring rod; 601. Driving groove; 602. First connecting seat; 603. Connecting strip; 604. Second connecting seat; 605. Driving sliding strip; 606. Stirring blade; 7. Top contact seat; 701. Return spring; 702. First locking seat; 703. Locking rod; 704. Second locking seat; 705. Locking claw. Detailed implementation manners

[0040] To make the objectives, technical solutions, and advantages of the embodiments of the present disclosure clearer, the technical solutions of the embodiments of the present disclosure will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present disclosure. Obviously, the described embodiments are part of the embodiments of the present disclosure, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the described embodiments of the present disclosure without creative efforts shall fall within the scope of protection of the present disclosure.

[0041] Please refer to Figure 1 - Figure 9 , an extraction device for selenium-rich green tea selenium protein, including a driving motor 103 and a driving rod 4 connected to the output end of the driving motor 103, further including a top seat 301. A converging groove 302 and a clamping groove 304 are provided on the lower surface of the top seat 301. One end of the driving rod 4 is connected with a driving assembly, and a stirring rod 6 and a fixed cylinder 5 are connected to the outside of the driving assembly. The driving rod 4 is arranged inside the stirring rod 6;

[0042] In a further embodiment provided by the present invention, a trigger assembly is provided inside the fixed cylinder 5, a release assembly is provided inside the stirring rod 6, a limiting groove is provided at the lower end of the fixed cylinder 5, the release assembly passes through the limiting groove and is connected to the trigger assembly. When the driving assembly moves, it drives the trigger assembly to expand and contract. When the trigger assembly extends to the longest, the locking assembly is triggered to lock the trigger assembly, and simultaneously drives the release assembly to release the connection between the driving assembly and the stirring rod 6.

[0043] In the present invention, by providing a release assembly to cooperate with the driving assembly to stir the mixed liquid, and when the driving assembly moves, it will also drive the trigger assembly to expand and contract. When the trigger assembly extends to the longest, the locking assembly is triggered to lock the trigger assembly, and simultaneously drives the release assembly to release the connection between the driving assembly and the stirring rod 6 to stop stirring the mixed liquid, so as to perform centrifugal treatment on the mixed liquid, enabling the device to automatically switch to the centrifugal state after passive stirring, and perform stirring and then centrifugation on the mixed liquid without transfer and automatically.

[0044] In a further embodiment provided by the present invention, the driving assembly includes a first screw rod 401 connected to the upper end of the driving rod 4, and a second screw rod 403 is sleeved at one end of the first screw rod 401. A first slot 402 is provided on the surface of the first screw rod 401, a protruding groove is provided inside the second screw rod 403, the protruding groove is slidably connected to the first slot 402, and a second slot 404 is provided on the surface of the second screw rod 403.

[0045] Furthermore, the protruding groove is a long strip-shaped protrusion, the upper half of the driving rod 4 is a screw rod structure, and the first screw rod 401 is sleeved outside the upper half of the driving rod 4.

[0046] Specifically, the rotation of the driving rod 4 drives the rotation of the first screw rod 401, the rotation of the first screw rod 401 synchronously drives the rotation of the second screw rod 403, and slides under the action of the first slot 402 and the second slot 404, so that the first screw rod 401 and the second screw rod 403 move upward and extend out.

[0047] In a further embodiment provided by the present invention, the trigger assembly includes a multi-stage telescopic cylinder 503 sleeved inside the fixed cylinder 5. A first inner groove 502 is provided on the inner surface of the fixed cylinder 5. A first sliding strip 504 is fixedly connected to the surface of the multi-stage telescopic cylinder 503, and the first sliding strip 504 is slidably matched with the first inner groove 502. A threaded hole 505 is provided at the lower end of the multi-stage telescopic cylinder 503. A second inner groove 506 is provided on the inner surface of the multi-stage telescopic cylinder 503. A locking cylinder 507 is sleeved at the upper end of the multi-stage telescopic cylinder 503. A second sliding strip 508 is fixedly connected to the surface of the locking cylinder 507, and the second sliding strip 508 is slidably matched with the second inner groove 506. The lower end of the second screw rod 403 is rotationally matched with the threaded hole 505.

[0048] Furthermore, the multi-stage telescopic cylinder 503 can be set with multiple stages according to actual requirements. In this embodiment, two stages are set.

[0049] Furthermore, a fixing strip 501 is connected to the surface of the fixed cylinder 5, and a connecting rod 511 is connected to the surface of the multi-stage telescopic cylinder 503.

[0050] Specifically, when the first screw rod 401 and the second screw rod 403 rotate, they drive the two stages in the multi-stage telescopic cylinder 503 to move synchronously, and under the action of the first inner groove 502, the movement is converted into a telescopic movement. That is, when the first screw rod 401 and the second screw rod 403 move, they drive the multi-stage telescopic cylinder 503 to perform telescopic movement synchronously, and under the action of the threaded hole 505 and the second inner groove 506, the locking cylinder 507 is further driven to perform telescopic movement.

[0051] In an embodiment further provided by the present invention, the locking assembly includes a top seat 7 slidably sleeved on the surface of the converging tube 303, and a return spring 701 is connected between the top seat 7 and the upper surface inside the converging groove 302. A first locking seat 702 is connected to the surface of the top seat 7. One end of the first locking seat 702 is rotatably connected to a locking rod 703, one end of the locking rod 703 is rotatably connected to a second locking seat 704, and a locking claw 705 is connected to one end of the second locking seat 704.

[0052] In an embodiment further provided by the present invention, a top contact groove 510 is provided at the upper end of the locking cylinder 507, a locking groove 509 is provided on the side surface of the locking cylinder 507, a converging tube 303 is fixedly connected inside the converging groove 302, the return spring 701 is provided on the surface of the converging tube 303, the converging tube 303 is slidably matched with the top contact groove 510, the locking claw 705 is located in the clamping groove 304 and is rotationally matched with the two side surfaces of the clamping groove 304, and the locking claw 705 is clamped and matched with the locking groove 509.

[0053] Specifically, when the locking cylinder 507 telescopically moves upward into the converging groove 302, the converging tube 303 enters the top contact groove 510, abuts against the top seat 7 and drives the top seat 7 to move upward, thereby driving the locking rod 703 to rotate, so as to drive the locking claw 705 to rotate, so that the claw-shaped structure at the front end of the locking claw 705 enters the locking groove 509 to lock the locking cylinder 507, thereby fixedly connecting the driving rod 4 and the top seat 301.

[0054] In a further embodiment provided by the present invention, a stirring blade 606 is fixedly connected to the surface of the stirring rod 6. A driving groove 601 is provided inside the stirring rod 6. The release assembly includes a first connecting seat 602 connected to the surface of the first screw rod 401. The lower end of the first connecting seat 602 is rotatably connected to a connecting bar 603. The lower end of the connecting bar 603 is rotatably connected to a second connecting seat 604. A driving slide bar 605 is slidably connected in the driving groove 601. One end of the second connecting seat 604 is fixedly connected to one side surface of the driving slide bar 605.

[0055] Further, the connecting bar 603 passes through the limiting groove and is slidably matched with the limiting groove.

[0056] Specifically, when the driving rod 4 moves, the first screw rod 401 drives the stirring rod 6 to rotate, so that the stirring blade 606 rotates to stir the mixed liquid. And during the continuous movement of the driving rod 4, the first screw rod 401 will also continuously move upward, thereby driving the first connecting seat 602 to move upward, so that the connecting bar 603 moves, pulling out the driving slide bar 605 from the driving groove 601, so that the driving rod 4 is no longer connected to the stirring rod 6, thereby stopping the stirring movement.

[0057] In a further embodiment provided by the present invention, it further includes a base 1. A support rod 101 is bolted to the upper surface of the base 1. The upper end of the support rod 101 is fixedly connected to a base 102. The base 102 is provided with a through hole. A driving motor 103 is arranged on the upper surface of the base 1. The output end of the driving motor 103 passes through the through hole and is connected to the lower end of the driving rod 4.

[0058] Further, a sensor is provided at the top of the converging groove 302. After the locking cylinder 507 contacts the sensor, the sensor controls the driving motor 103 to increase the speed to 3600 r / min.

[0059] In a further embodiment provided by the present invention, an outer shell 3 is rotatably connected to the upper surface of the base 1. The upper end of the outer shell 3 is fixedly connected to a top seat 301. A feed pipe 305 communicates with the upper surface of the top seat 301. Inside the outer shell 3, an inner shell 306, a filter frame 307 and a mask expansion plate 308 are arranged in sequence from outside to inside. The inner shell 306 and the filter frame 307 are rotatably connected to the base 1. The upper end of the mask expansion plate 308 is fixedly connected to the lower surface of the top seat 301.

[0060] Further, the mask expansion plate 308 is divided into two parts. The lower half can be received inside the upper half. The lower half is provided with a first installation groove, and the upper half is provided with a second installation groove.

[0061] Further, the fixing bar 501 passes through the first installation groove and is fixedly connected to the filter frame 307, and the fixing bar 501 is slidably engaged with the first installation groove. The connecting rod 511 passes through the second installation groove and is fixedly connected to the top end of the lower half of the mask expansion plate 308, and the connecting rod 511 is slidably engaged with the second installation groove.

[0062] Specifically, when the connecting rod 511 moves upward, it synchronously slides along the second installation groove to drive the lower half of the mask expansion plate 308 to slide and be received into the upper half. While scraping the mixed liquid adhered thereto, the filter frame 307 is exposed. After the driving rod 4 is fixedly connected to the top seat 301, since the inner shell 306 and the filter frame 307 are rotatably connected to the base 1, after locking, the driving rod 4 drives the inner shell 306 and the filter frame 307 to rotate, thereby driving the mixed liquid inside to rotate rapidly and performing centrifugal treatment through the filter frame 307.

[0063] In an embodiment further provided by the present invention, a heating coil 202 is provided between the outer shell 3 and the inner shell 306. A circuit tube 201 is provided on the surface of the heating coil 202, and a heater 2 is provided on the upper surface of the base 1. One end of the circuit tube 201 penetrates through the outer shell 3 and is connected to the heater 2.

[0064] A method for extracting selenium protein from selenium-rich green tea is extracted by the above-mentioned extraction device for selenium protein from selenium-rich green tea, and includes the following steps:

[0065] S1. Feeding: After adding tea powder into the device through the feed pipe 305, add 0.1 mol / L sodium hydroxide solution, and ensure that the material-liquid ratio of the tea powder to the solution is 1:60 g / mL;

[0066] S2. Stirring extraction: After starting the heater 2 to heat the temperature of the mixed liquid to 69 °C, start the driving motor 103 to stir the mixed liquid for 3 h;

[0067] S3. Centrifugal treatment: After stirring, perform centrifugal treatment on the mixed liquid for 10 min, and the centrifugal rate is 3600 r / min;

[0068] S4. pH adjustment: After centrifugation, add acid solution through the feed pipe 305 to adjust the pH to 2.0, and let it stand for a period of time to precipitate proteins;

[0069] S5. Secondary centrifugation: Perform centrifugal treatment on the mixed liquid again for 10 min, and the centrifugal rate is 3600 r / min;

[0070] S6. Extraction completion: Suck out the upper clear liquid and discard it. Take out the lower solid mass and put it into a vacuum dryer for drying to obtain a selenium protein sample.

[0071] In the present invention, tea powder is added into the mask telescopic plate 308 through the feed pipe 305, and then a 0.1 mol / L sodium hydroxide solution is added, ensuring that the material-liquid ratio of the tea powder to the solution is 1:60 g / mL. Then, the heater 2 is started. Under the action of the heating coil 202, the temperature of the mixed liquid is heated to 69 °C. After that, the driving motor 103 is started to drive the driving rod 4 to rotate, so that the first spiral rod 401 drives the stirring rod 6 to rotate, and the stirring blade 606 rotates to stir the mixed liquid. During the continuous movement of the driving rod 4, the first spiral rod 401 will also continuously move upward, driving the first connecting seat 602 to move upward, so that the connecting bar 603 moves, pulling the driving slider 605 out of the driving groove 601, disconnecting the driving rod 4 from the stirring rod 6, and thus stopping the stirring motion. Synchronously, the rotation of the driving rod 4 drives the first spiral rod 401 to rotate. The rotation of the first spiral rod 401 synchronously drives the second spiral rod 403 to rotate and move upward. When the first spiral rod 401 and the second spiral rod 403 rotate, they drive the multi-stage telescopic cylinder 503 and the locking cylinder 507 to extend. When the locking cylinder 507 extends, it drives the connecting rod 511 to move upward. Synchronously, the connecting rod 511 slides along the second installation groove, driving the lower half of the mask telescopic plate 308 to slide and be received into the upper half. While scraping the adhered mixed liquid on it, the filter frame 307 is exposed. When the locking cylinder 507 continuously extends upward into the converging groove 302, the converging pipe 303 enters the abutting groove 510 and abuts against the abutting seat 7, driving the abutting seat 7 to move upward, thereby driving the locking rod 703 to rotate, driving the locking claw 705 to rotate, so that the claw-shaped structure at the front end of the locking claw 705 enters the locking groove 509 to lock the locking cylinder 507, and thus fixedly connecting the driving rod 4 and the top seat 301. After the driving rod 4 and the top seat 301 are fixedly connected, since the inner shell 306 and the filter frame 307 are rotatably connected to the base 1, after locking, the driving rod 4 drives the inner shell 306 and the filter frame 307 to rotate, driving the mixed liquid inside to rotate rapidly and performing centrifugal treatment through the filter frame 307. That is, the complete movement stroke is: the driving motor 103 first drives the stirring blade 606 to move. After the first spiral rod 401 extends to a certain length, the connection between the driving motor 103 and the stirring blade 606 is released, the stirring stops, and synchronously, the locking cylinder 507 is driven to extend into the converging groove 302, connecting the driving rod 4 and the top seat 301, and performing centrifugal treatment.

[0072] The above only describes certain exemplary embodiments of the present invention by way of illustration. Undoubtedly, for those of ordinary skill in the art, without departing from the spirit and scope of the present invention, the described embodiments can be modified in various different ways. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. A device for extracting selenoprotein from selenium-rich green tea, comprising a driving motor (103) and a driving rod (4) connected to the output end of the driving motor (103), characterized in that: It also comprises a top seat (301), the lower surface of which is provided with a gathering groove (302) and a clamping groove (304), one end of the driving rod (4) is connected to a driving assembly, the outside of the driving assembly is connected to a stirring rod (6) and a fixing cylinder (5), and the driving rod (4) is arranged inside the stirring rod (6); A locking assembly is provided in the convergence groove (302), a trigger assembly is provided in the fixed cylinder (5), a release assembly is provided inside the stirring rod (6), a limiting groove is provided at the lower end of the fixed cylinder (5), the release assembly passes through the limiting groove and is connected to the trigger assembly, and when the driving assembly moves, the trigger assembly is driven to extend and retract, and when the trigger assembly is extended to the longest, the locking assembly is triggered to lock the trigger assembly, and the release assembly is synchronously driven to release the connection between the driving assembly and the stirring rod (6).

2. The device for extracting selenium-enriched green tea selenoprotein according to claim 1, characterized in that: The driving assembly comprises a first screw rod (401) connected to the upper end of the driving rod (4), and a second screw rod (403) is sleeved on one end of the first screw rod (401), and a first groove (402) is provided on the surface of the first screw rod (401), and a protruding groove is provided inside the second screw rod (403), and the protruding groove is slidably connected to the first groove (402), and a second groove (404) is provided on the surface of the second screw rod (403).

3. The extraction device of selenium-enriched green tea selenoprotein according to claim 2, characterized in that: The trigger assembly includes a multi-stage telescopic cylinder (503) sleeved inside a fixed cylinder (5), and a first inner groove (502) is provided on the inner surface of the fixed cylinder (5), and a first slide bar (504) is fixedly connected to the surface of the multi-stage telescopic cylinder (503), and the first slide bar (504) is slidably matched with the first inner groove (502), and a threaded hole (505) is provided at the lower end of the multi-stage telescopic cylinder (503), and a second inner groove (506) is provided on the inner surface of the multi-stage telescopic cylinder (503), and a locking cylinder (507) is sleeved on the upper end of the multi-stage telescopic cylinder (503), and a second slide bar (508) is fixedly connected to the surface of the locking cylinder (507), and the second slide bar (508) is slidably matched with the second inner groove (506), and the lower end of the second screw rod (403) is rotationally matched with the threaded hole (505).

4. The device for extracting selenium-enriched green tea selenoprotein according to claim 3, characterized in that: The locking assembly comprises a top-connecting seat (7) slidably sleeved on the surface of the convergence tube (303), and a return spring (701) is connected between the top-connecting seat (7) and the inner upper surface of the convergence groove (302), and the surface of the top-connecting seat (7) is connected to a first locking seat (702), and one end of the first locking seat (702) is rotatably connected to a locking rod (703), and one end of the locking rod (703) is rotatably connected to a second locking seat (704), and one end of the second locking seat (704) is connected to a locking claw (705).

5. The device for extracting selenium-enriched green tea selenoprotein according to claim 4, characterized in that: The upper end of the locking cylinder (507) is provided with a top connection groove (510), and the side surface of the locking cylinder (507) is provided with a locking groove (509). The convergence groove (302) is fixedly connected with a convergence tube (303), and the return spring (701) is provided on the surface of the convergence tube (303). The convergence tube (303) is slidably matched with the top connection groove (510). The locking claw (705) is located in the clamping groove (304) and is rotationally matched with the two side surfaces of the clamping groove (304). The locking claw (705) is clamped and matched with the locking groove (509).

6. The device for extracting selenium-enriched green tea selenoprotein according to claim 5, characterized in that: A stirring blade (606) is fixedly connected to the surface of the stirring rod (6), and a driving groove (601) is provided inside the stirring rod (6). The release component includes a first connecting seat (602) connected to the surface of the first spiral rod (401), and the lower end of the first connecting seat (602) is rotatably connected to a connecting strip (603), and the lower end of the connecting strip (603) is rotatably connected to a second connecting seat (604), and a driving slide bar (605) is slidably connected in the driving groove (601), and one end of the second connecting seat (604) is fixedly connected to the surface of one side of the driving slide bar (605).

7. The device for extracting selenium-enriched green tea selenoprotein according to claim 6, characterized in that: It also comprises a base (1), the upper surface of the base (1) being connected to a support rod (101) by bolts, the upper end of the support rod (101) being fixedly connected to a base (102), the base (102) being provided with a through hole, the drive motor (103) being arranged on the upper surface of the base (1), the output end of the drive motor (103) passing through the through hole and connected to the lower end of the drive rod (4).

8. The device for extracting selenium-enriched green tea selenoprotein according to claim 7, characterized in that: The upper surface of the base (1) is rotatably connected to an outer shell (3), the upper end of the outer shell (3) is fixedly connected to the top seat (301), the upper surface of the top seat (301) is connected to a feed pipe (305), the inner shell (306), a filter frame (307) and a shield telescopic plate (308) are arranged in sequence inside the outer shell (3) from the outside to the inside, the inner shell (306) and the filter frame (307) are rotatably connected to the base (1), and the upper end of the shield telescopic plate (308) is fixedly connected to the lower surface of the top seat (301).

9. The device for extracting selenium-enriched green tea selenoprotein according to claim 8, characterized in that: A heating coil (202) is arranged between the outer shell (3) and the inner shell (306), a circuit tube (201) is arranged on the surface of the heating coil (202), a heater (2) is arranged on the upper surface of the base (1), and one end of the circuit tube (201) passes through the outer shell (3) and is connected to the heater (2).

10. A method for extracting selenium-enriched green tea selenoprotein, using the selenium-enriched green tea selenoprotein extraction device according to any one of claims 1 to 9, characterized in that: The steps include: S1, feeding: after tea powder is added into the device through the feeding pipe (305), 0.1 mol / L sodium hydroxide solution is added, and the material-liquid ratio of tea powder to solution is ensured to be 1:60 g / mL; S2, stirring and leaching: start the heater (2) to heat the mixed solution to 69° C., then start the drive motor 103 to stir the mixed solution for 3 hours; S3, centrifugal treatment: after the stirring is completed, the mixed solution is centrifuged for 10 minutes at a centrifugal speed of 3600 r / min; S4, pH adjustment: After centrifugation, acid solution is added through the feed tube (305) to adjust the pH to 2.0, and the mixture is allowed to stand for a period of time to precipitate protein; S5, secondary centrifugation: centrifuge the mixed solution again for 10 min at a centrifugal speed of 3600 r / min; S6. Extraction is completed: the upper clear liquid is sucked out and discarded, and the lower solid matter is taken out and placed in a vacuum dryer for drying to obtain a selenoprotein sample.

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