Hawthorn spider mite control agent taking CGG as carrier and preparation device of hawthorn spider mite control agent

Abstract

The invention relates to the technical field of pest mite biopesticide prevention and control, in particular to a hawthorn tetranychid mite prevention and control agent with CGG as a carrier and a preparation device.The hawthorn tetranychid mite prevention and control agent with CGG as the carrier comprises a base, a metering pump, a supporting table, a batching structure, a defoaming structure and a mixing structure, and defoaming barrels are symmetrically and fixedly connected to one side of the top of the base; the other side of the top of the base is fixedly connected with a mixing barrel, the top of the partition plate is symmetrically and fixedly connected with metering pumps, the two sides of the base are fixedly connected with supporting tables, the tops of the supporting tables are fixedly connected with a batching barrel, a batching structure used for batching is installed in the batching barrel, and a defoaming structure used for defoaming is installed in a supporting plate. The hawthorn tetranychus mite prevention and control agent with the CGG as the carrier and the preparation device of the hawthorn tetranychus mite prevention and control agent have the advantages that the prevention and control effect of hawthorn tetranychus mites is improved, efficient automatic production is achieved, efficient defoaming treatment is achieved, and cleaning and maintenance are easy.

Description

Technical Field

[0001] The present invention relates to the technical field of biological pesticide control of harmful mites, and particularly relates to a Tetranychus viennensis control agent with CGG as a carrier and a preparation device thereof. Background Art

[0002] With the continuous growth of the global population and the intensification of climate change, agricultural production is facing unprecedented challenges, especially the effective prevention and control of pests and pathogens. Finding environmentally friendly and efficient prevention and control technologies has become an urgent need for the sustainable development of agriculture. RNA interference (RNAi) technology, with its high efficiency, specificity, and non-residual nature, has opened up a new path for the prevention and control of agricultural pests and pathogens. This technology specifically degrades the mRNA of target pests or pathogens, thereby blocking the expression of their key proteins, and then effectively controlling their growth, reproduction, or pathogenicity. However, in practical applications, the core molecule of RNAi technology, double-stranded RNA (dsRNA), has encountered problems of low delivery efficiency and poor stability, which have greatly restricted the wide application and popularization of RNAi technology. To solve these problems, researchers have continuously explored various novel delivery carriers, including liposomes, polymer nanoparticles, and inorganic nanoparticles, etc. Although these carriers have shown certain advantages in some aspects, there are still limitations such as insufficient stability, high cost, complex preparation, or poor control effects. In this context, guar gum (GG), as a natural high-molecular polysaccharide, has gradually entered the research field of dsRNA delivery carriers due to its excellent biocompatibility, biodegradability, and low toxicity. Especially cationic guar gum (CGG) obtained through chemical modification has strong electrostatic interactions and can efficiently bind to dsRNA. The cationic polymer carries a positive charge, while the phosphate backbone of dsRNA carries a negative charge. The electrostatic attraction between the two can achieve rapid and stable binding, improving the loading efficiency of the carrier. This binding can reduce the degradation of dsRNA in the in vitro environment, especially reducing the degradation risk of nucleases and prolonging the effective time of dsRNA. The cationic polymer can promote the passage of dsRNA through the cell membrane, mainly due to the interaction between the cationic polymer and the negative charge of the cell membrane, thus promoting the endocytosis of dsRNA. Guar gum (CGG) has natural adhesiveness and can form a thin film on the leaf surface, which helps to improve the delivery efficiency and stability of dsRNA in agricultural applications. Cationic guar gum (CGG) has low toxicity, environmental friendliness, high binding ability, and economy in the application of dsRNA carriers, and is a very promising material, especially suitable for large-scale spraying applications in the agricultural field. A new control agent based on cationic guar gum (CGG) hydrogel encapsulating V-ATPase A dsRNA is proposed for the agricultural pest Tetranychus viennensis. V-ATPase A is a key protein in the growth and reproduction process of Tetranychus viennensis. The specific degradation of its mRNA can effectively inhibit the growth and reproduction of Tetranychus viennensis. As a delivery carrier, the cationic guar gum (CGG) hydrogel can not only efficiently deliver V-ATPase A dsRNA into Tetranychus viennensis, but also achieve controlled release of dsRNA through its gel properties, thereby prolonging the action time of RNAi and improving the control effect; When producing this control agent, air bubbles will be generated inside both the cationic guar gum (CGG) solution and the V-ATPase A dsRNA aqueous solution, which will affect the accuracy of subsequent quantitative mixing of the cationic guar gum (CGG) solution and the V-ATPase A dsRNA aqueous solution. In addition, after each process, the inner wall of the batching tank often adheres to the solution. When preparing a cationic guar gum (CGG) solution and a V-ATPase A dsRNA aqueous solution with a certain concentration, the solution adhering to the inner wall of the batching tank often affects the concentration of the cationic guar gum (CGG) solution and the V-ATPase A dsRNA aqueous solution.

[0003] Therefore, it is necessary to provide a new preparation device for the control agent of Tetranychus viennensis with CGG as the carrier to solve the above technical problems. Summary of the Invention

[0004] In order to solve the above technical problems, the present invention provides a hawthorn spider mite control agent using CGG as a carrier and a preparation device thereof.

[0005] The device for preparing a hawthorn spider mite control agent with CGG as a carrier provided by the present invention comprises: a base, a metering pump, a support platform, a batching structure, a defoaming structure and a mixing structure, a partition is fixedly connected to the top of the base, a defoaming barrel is symmetrically fixedly connected to the top of the base located on one side of the partition, a mixing barrel is fixedly connected to the top of the base located on the other side of the partition, a metering pump is symmetrically fixedly connected to the top of the partition, an input end of the metering pump is connected to the bottom of the corresponding defoaming barrel through a pipeline, an output end of the metering pump is connected to the top of the mixing barrel through a pipeline, support platforms are fixedly connected to both sides of the base, a batching barrel is fixedly connected to the top of the support platform, the bottom of the batching barrel is connected to the top of the defoaming barrel through a pipeline, a support plate is fixedly connected to the top of the support platform between the two batching barrels, a T-shaped support column is fixedly connected to the top of the support plate, a batching structure for batching is installed inside the batching barrel, a defoaming structure for defoaming is installed inside the support plate, and a mixing structure for mixing is installed inside the mixing barrel.

[0006] Preferably, the dosing structure includes: a turntable, a dosing worm wheel, a dosing worm, a first motor, an electric push rod, a scraper and a three-way valve. The bottom of the two dosing barrels are rotatably connected to the turntable, the bottom of the turntable is fixedly connected to the dosing worm wheel, one side of the dosing barrel is provided with an opening, the top of the support table is rotatably connected to the dosing worm, the dosing worm is meshingly connected to the dosing worm wheel, the inside of one side of the support table is fixedly connected to the first motor, the output end of the first motor is fixedly connected to one end of the dosing worm, both ends of the T-shaped support column are fixedly connected to the electric push rod, the inner wall of the dosing barrel is slidably connected to the scraper, the output end of the electric push rod is fixedly connected to the top of the scraper, and the bottom of the dosing barrel is provided with a three-way valve for controlling the flow direction of the solution.

[0007] Preferably, the defoaming structure includes: a third motor, a defoaming worm, a defoaming worm wheel, a gear, a first connecting arm, a connecting rod, a second connecting arm, a driving pulley, a driven pulley, a cylindrical rack and a defoaming column. The third motor is fixedly connected inside the support plate, and the output end of the third motor is fixedly connected to the defoaming worm. The support plate is symmetrically and rotatably connected to the defoaming worm wheel, and the defoaming worm wheel is meshed with the defoaming worm. The support plate is symmetrically and rotatably connected to the gear. One side of the defoaming worm wheel is fixedly connected to the first connecting arm, one end of the first connecting arm is rotatably connected to the connecting rod, one side of the gear is fixedly connected to the second connecting arm, one end of the connecting rod is rotatably connected to the second connecting arm, the outer wall of the defoaming worm is fixedly connected to the driving pulley, and the support plate is symmetrically and rotatably connected to the driven pulley. The driving pulley is connected to the driven pulley through a belt, the inner wall of the driven pulley is slidably connected to a cylindrical rack, the cylindrical rack is meshed with the gear, and a plurality of groups of defoaming columns are equidistantly fixedly connected to the bottom of the cylindrical rack.

[0008] Preferably, the mixing structure includes: a fourth motor, a mixing column, and a switching valve. The fourth motor is fixedly connected inside the base. The output end of the fourth motor is fixedly connected to the mixing column. The bottom of the mixing barrel is fixedly connected to a discharge pipe, and a switching valve is installed in the middle of the discharge pipe.

[0009] Preferably, the batching worm gear and the turntable are provided with through holes at the axis. The diameter of the through hole is the same as the diameter of the pipe communicating with the bottom of the batching barrel.

[0010] Preferably, both of the three-way valves are composed of a housing, a fluid guide body, and a rotating rod. The housing is provided at the bottom of the batching barrel. The housing is fixedly connected to the pipe. The inner wall of the housing is rotatably connected to the fluid guide body. One side of the fluid guide body is fixedly connected to the rotating rod. The end of the rotating rod away from the fluid guide body is fixedly connected to a transmission belt pulley. The two transmission belt pulleys are connected by a belt drive. The second motor is fixedly connected to the side of the support platform away from the first motor. The output end of the second motor is fixedly connected to the transmission belt pulley away from the first motor.

[0011] Preferably, the outer wall of the cylindrical rack is provided with external splines, and the inner wall of the driven belt pulley is provided with internal splines that are fitted and installed with the external splines.

[0012] Preferably, the second connecting arm is longer than the first connecting arm.

[0013] Preferably, a plurality of defoaming needles are equidistantly fixedly connected to one side of the plurality of defoaming columns, and the plurality of defoaming columns are installed in a staggered manner.

[0014] The present invention also discloses a preparation method of a fracturing crosslinking agent, and the method includes the following steps: S1: The present invention provides a Tetranychus viennensis Zacher control agent using cationic guar gum (CGG) as a carrier. The control agent uses cationic guar gum (CGG) as a carrier to encapsulate V-ATPase A dsRNA. Cationic guar gum (CGG) and water are added to one of the batching barrels at a mass ratio of 1% to 2%. A solution containing V-ATPase A dsRNA and water are added to the other batching barrel at a mass ratio of 0.3% to 0.7%. S2: Start the first motor. The first motor drives the batching worm to rotate. The batching worm gear drives the batching worm gear to rotate, and then drives the turntable to rotate to stir the solution in the batching barrel. S3: Start the second motor. The second motor drives the transmission belt pulley to rotate, and then drives the rotating rod and the fluid guide body to rotate, so that the batching barrel is communicated with the defoaming barrel, and the solution is sent into the defoaming barrel. S4: Start the third motor. The third motor drives the defoaming worm to rotate, which in turn drives the driving pulley and the defoaming worm gear to rotate. The driving pulley drives the driven pulley to rotate through the belt. The driven pulley drives the cylindrical rack to rotate. The cylindrical rack drives the defoaming column to rotate. The defoaming worm gear drives the first connecting arm to rotate. During the rotation of the first connecting arm, the second connecting arm and the gear are driven to reciprocate through the connecting rod. The gear drives the cylindrical rack to move reciprocally in the vertical direction. The cylindrical rack drives the defoaming column to move reciprocally in the vertical direction; S5: Start the metering pump. The metering pump pumps the cationic guar gum (CGG) and the dsRNA aqueous emulsion from the batching tank into the mixing tank. Start the fourth motor. The fourth motor drives the mixing column to rotate to stir the solution in the mixing tank; S6: Start the second motor. The second motor drives the transmission pulley to rotate, which in turn drives the rotating rod and the deflector to rotate, connecting the batching tank with the waste pipe. Start the electric push rod. The electric push rod drives the scraping disc to move downward and scrape off the solution adhering to the inner wall of the batching tank.

[0015] Compared with the related technologies, the preparation device of the hawthorn spider mite control agent with CGG as the carrier provided by the present invention has the following beneficial effects: Improve the control effect on hawthorn spider mites: The preparation obtained by the production method mentioned in the present invention has good stability, significantly improves the control effect on the target hawthorn spider mites, and at the same time improves the stability of dsRNA, extends the degradation time of dsRNA in nature, and has simple loading and simple and easy-to-obtain carriers; High-efficiency and automated production: The device integrates multiple functions such as batching, defoaming, and mixing, realizing a fully automated process from raw material input to finished product output, significantly improving production efficiency. Through the precise control of equipment such as motors and metering pumps, the accuracy and stability of each step in the preparation process are ensured; High-efficiency defoaming treatment: The defoaming structure adopts designs such as defoaming worm, defoaming worm gear, gear, cylindrical rack, and defoaming column, enabling the defoaming column to rotate in the defoaming tank while reciprocating vertically, achieving efficient and rapid elimination of bubbles in the solution, avoiding the influence of bubbles on the effect of the control agent. The misaligned installation and reciprocating movement design of the defoaming column further improve the defoaming efficiency; Easy to clean and maintain: The scraping disc structure installed inside the batching tank can scrape off the solution adhering to the inner wall of the batching tank through the drive of the electric push rod, avoiding equipment pollution and reducing maintenance costs; Ensure accurate proportioning: Through the precise control of the electric push rod, the scraper structure can closely fit the inner wall of the batching barrel, effectively scraping off the residual adhered solution. This function ensures the cleanliness of the barrel after each batching, avoiding the interference of the residual solution on the subsequent ratio, thus guaranteeing the precise proportion and stability of the components of the control agent.

[0016] Efficient delivery and enhanced stability: CGG forms a stable complex through electrostatic adsorption, protecting dsRNA from degradation and enhancing its absorption efficiency in Tetranychus viennensis. The biological activity is increased by more than 30%; Low cost and convenience: CGG is a natural renewable material, with the raw material cost only 1 / 5 of that of synthetic carriers, and the preparation process is simplified, without the need for complex equipment, suitable for large-scale production; Broad application prospects: This technology can be extended to other agricultural pest control fields, such as aphids, spider mites, etc., providing reliable carrier support for the field application of RNAi technology. Description of the drawings

[0017] Figure 1 It is a schematic structural diagram of the preparation device of the control agent for Tetranychus viennensis with CGG as the carrier provided by the present invention; Figure 2 For Figure 1 The schematic back structure diagram of the preparation device of the control agent for Tetranychus viennensis with CGG as the carrier shown; Figure 3 For Figure 2 The schematic internal structure diagram of the support platform shown; Figure 4 For Figure 2 The schematic sectional structure diagram of the batching barrel shown; Figure 5 For Figure 2 The schematic sectional structure diagram of the three-way valve shown; Figure 6 For Figure 2 One of the schematic sectional structure diagrams of the support plate shown; Figure 7 For Figure 2 The other schematic sectional structure diagram of the support plate shown; Figure 8 For Figure 1 The schematic sectional structure diagram of the base shown.

[0018] Reference Numerals in the Figures: 1, base; 2, partition; 3, defoaming barrel; 4, mixing barrel; 5, metering pump; 6, support platform; 7, batching barrel; 8, support plate; 9, T-shaped support column; 10, turntable; 11, batching worm gear; 12, batching worm; 13, first motor; 14, electric push rod; 15, scraping disc; 16, three-way valve; 17, third motor; 18, defoaming worm; 19, defoaming worm gear; 20, gear; 21, first connecting arm; 22, connecting rod; 23, second connecting arm; 24, driving pulley; 25, driven pulley; 26, cylindrical rack; 27, defoaming column; 28, fourth motor; 29, mixing column; 30, switching valve; 31, housing; 32, fluid guide; 33, rotating rod; 34, driving belt pulley; 35, second motor. Detailed Implementation Manner

[0019] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0020] The following describes the specific implementation of the present invention in detail in conjunction with specific embodiments.

[0021] Please refer to Figures 1 to 8 , a preparation device for a hawthorn spider mite control agent with CGG as a carrier. The preparation device for a hawthorn spider mite control agent with CGG as a carrier includes: a base 1, a metering pump 5, a support platform 6, a batching structure, a defoaming structure, and a mixing structure. A partition 2 is fixedly connected to the top of the base 1. Defoaming barrels 3 are symmetrically and fixedly connected to one side of the partition 2 on the top of the base 1. A mixing barrel 4 is fixedly connected to the other side of the partition 2 on the top of the base 1. Metering pumps 5 are symmetrically and fixedly connected to the top of the partition 2. The input end of the metering pump 5 is communicated with the bottom of the corresponding defoaming barrel 3 through a pipeline, and the output end of the metering pump 5 is communicated with the top of the mixing barrel 4 through a pipeline. Support platforms 6 are fixedly connected to both sides of the base 1. A batching barrel 7 is fixedly connected to the top of the support platform 6. The bottom of the batching barrel 7 is communicated with the top of the defoaming barrel 3 through a pipeline. A support plate 8 is fixedly connected between the two batching barrels 7 on the top of the support platform 6. A T-shaped support column 9 is fixedly connected to the top of the support plate 8. A batching structure for batching is installed inside the batching barrel 7. A defoaming structure for defoaming is installed inside the support plate 8. A mixing structure for mixing is installed inside the mixing barrel 4. The mixing structure includes: a fourth motor 28, a mixing column 29, and a switching valve 30. A fourth motor 28 is fixedly connected inside the base 1. The output end of the fourth motor 28 is fixedly connected to a mixing column 29. A discharge pipe is fixedly connected to the bottom of the mixing barrel 4, and a switching valve 30 is installed in the middle of the discharge pipe; It should be noted that: the metering pump 5 accurately extracts and transports the solution in the defoaming barrel 3 into the mixing barrel 4; Please refer toFigures 2 to 5 The batching structure includes: a turntable 10, a batching worm wheel 11, a batching worm 12, a first motor 13, an electric push rod 14, a scraper 15 and a three-way valve 16. The bottoms of the two batching barrels 7 are rotatably connected with the turntable 10, the bottom of the turntable 10 is fixedly connected with the batching worm wheel 11, one side of the batching barrel 7 is provided with an opening, the top of the support platform 6 is rotatably connected with the batching worm 12, the batching worm 12 is meshed with the batching worm wheel 11, one side of the support platform 6 is fixedly connected with the first motor 13, the output end of the first motor 13 is fixedly connected to one end of the batching worm 12, both ends of the T-shaped support column 9 are fixedly connected with the electric push rod 14, the inner wall of the batching barrel 7 is slidably connected with the scraper 15, the output end of the electric push rod 14 is fixedly connected to the top of the scraper 15, and the bottom of the batching barrel 7 is provided with A three-way valve 16 for controlling the flow direction of the solution, a through hole is provided at the axis of the dosing worm wheel 11 and the turntable 10, the diameter of the through hole is the same as the diameter of the pipeline connected to the bottom of the dosing barrel 7, and the two three-way valves 16 are composed of a shell 31, a guide body 32 and a rotating rod 33. The bottom of the dosing barrel 7 is provided with a shell 31, the shell 31 is fixedly connected to the pipeline, the inner wall of the shell 31 is rotatably connected to the guide body 32, and one side of the guide body 32 is fixedly connected to the rotating rod 33, and the end of the rotating rod 33 away from the guide body 32 is fixedly connected to a driving pulley 34, and the two driving pulleys 34 are connected by belt transmission, and the side of the support platform 6 away from the first motor 13 is fixedly connected to the second motor 35, and the output end of the second motor 35 is fixedly connected to the driving pulley 34 away from the first motor 13; It should be noted that the solution can flow into the pipeline through the through hole opened at the axis of the dosing worm wheel 11 and the turntable 10, and then flow into the defoaming barrel 3. The first motor 13 and the second motor 35 are respectively located on both sides of the support platform 6, which is conducive to the heat dissipation of the first motor 13 and the second motor 35. See also Figure 2 , Figure 6 and Figure 7, the defoaming structure includes: a third motor 17, a defoaming worm 18, a defoaming worm gear 19, a gear 20, a first connecting arm 21, a connecting rod 22, a second connecting arm 23, a driving pulley 24, a driven pulley 25, a cylindrical rack 26 and defoaming columns 27. A third motor 17 is fixedly connected inside the support plate 8. The output end of the third motor 17 is fixedly connected with a defoaming worm 18. Two defoaming worm gears 19 are symmetrically and rotatably connected inside the support plate 8. The defoaming worm gears 19 are meshed with the defoaming worm 18. Two gears 20 are symmetrically and rotatably connected inside the support plate 8. One side of the defoaming worm gear 19 is fixedly connected with a first connecting arm 21. One end of the first connecting arm 21 is rotatably connected with a connecting rod 22. One side of the gear 20 is fixedly connected with a second connecting arm 23. One end of the connecting rod 22 is rotatably connected with the second connecting arm 23. The outer wall of the defoaming worm 18 is fixedly connected with a driving pulley 24. Two driven pulleys 25 are symmetrically and rotatably connected inside the support plate 8. The driving pulley 24 is drivingly connected with the driven pulley 25 through a belt. A cylindrical rack 26 is slidably connected to the inner wall of the driven pulley 25. The cylindrical rack 26 is meshed with the gear 20. Multiple groups of defoaming columns 27 are equidistantly fixedly connected to the bottom of the cylindrical rack 26. External splines are provided on the outer wall of the cylindrical rack 26, and internal splines for mating installation with the external splines are provided on the inner wall of the driven pulley 25. The second connecting arm 23 is longer than the first connecting arm 21. Multiple defoaming needles are equidistantly fixedly connected to one side of the multiple groups of defoaming columns 27. The multiple groups of defoaming columns 27 are staggeredly installed; It should be noted that: the mating installation of the internal splines and the external splines enables the cylindrical rack 26 to rotate as the driven pulley 25 rotates when sliding on the inner wall of the driven pulley 25. Driven by the first connecting arm 21, the second connecting arm 23 and the connecting rod 22, the defoaming worm gear 19 drives the gear 20 to rotate reciprocally through its own rotation. The design of the staggered installation of the defoaming columns 27 further improves the defoaming efficiency; Please refer to Figure 2 and Figure 8 , the present invention provides a preparation device for a Tetranychus viennensis control agent using CGG as a carrier. The components of the control agent are as follows: a cationic guar gum (CGG) aqueous emulsion with a mass ratio of 1% - 2%, and a V - ATPaseA dsRNA aqueous emulsion with a mass ratio of 0.3% - 0.7%; the preparation method is as follows: S1: The present invention provides a Tetranychus viennensis control agent using cationic guar gum (CGG) as a carrier. The control agent uses cationic guar gum (CGG) as a carrier to encapsulate V - ATPase A dsRNA. Cationic guar gum (CGG) and water are added to one of the ingredient barrels 7 at a mass ratio of 1% - 2%, and a solution containing V - ATPase A dsRNA and water are added to another ingredient barrel 7 at a mass ratio of 0.3% - 0.7%; S2: Start the first motor 13. The first motor 13 drives the batching worm 12 to rotate. The batching worm wheel 11 drives the batching worm wheel 11 to rotate, and then drives the turntable 10 to rotate to stir the solution in the batching barrel 7. S3: Start the second motor 35. The second motor 35 drives the transmission pulley 34 to rotate, and then drives the rotating rod 33 and the defoaming guide 32 to rotate, so that the batching barrel 7 is connected to the defoaming barrel 3, and the solution is sent into the defoaming barrel 3. S4: Start the third motor 17. The third motor 17 drives the defoaming worm 18 to rotate, and then drives the driving pulley 24 and the defoaming worm wheel 19 to rotate. The driving pulley 24 drives the driven pulley 25 to rotate through the belt. The driven pulley 25 drives the cylindrical rack 26 to rotate. The cylindrical rack 26 drives the defoaming column 27 to rotate. The defoaming worm wheel 19 drives the first connecting arm 21 to rotate. During the rotation of the first connecting arm 21, the second connecting arm 23 and the gear 20 are driven to rotate reciprocally through the connecting rod 22. The gear 20 drives the cylindrical rack 26 to perform reciprocating movement in the vertical direction. The cylindrical rack 26 drives the defoaming column 27 to perform reciprocating movement in the vertical direction. S5: Start the metering pump 5. The metering pump 5 pumps the cationic guar gum (CGG) and the dsRNA aqueous emulsion from the batching barrel 7 into the mixing barrel 4. Start the fourth motor 28. The fourth motor 28 drives the mixing column 29 to rotate to stir the solution in the mixing barrel 4. S6: Start the second motor 35. The second motor 35 drives the transmission pulley 34 to rotate, and then drives the rotating rod 33 and the defoaming guide 32 to rotate, so that the batching barrel 7 is connected to the waste pipe. Start the electric push rod 14. The electric push rod 14 drives the scraping disc 15 to move downward and scrape off the solution adhering to the inner wall of the batching barrel 7.

[0022] It should be noted that: Through the electrostatic binding of CGG and dsRNA, the stability and delivery efficiency of dsRNA are significantly improved. The cationic property of CGG can efficiently bind to negatively charged dsRNA to form a stable complex, effectively resisting nuclease degradation and prolonging the action time. The natural adhesiveness of CGG can form a uniform film on the leaf surface, promoting the absorption of dsRNA through the body wall of spider mites and enhancing biological activity. Compared with traditional liposomes or nanocarriers, CGG has low cost, easy availability of raw materials, and simple preparation process, and is suitable for large-scale agricultural applications.

[0023] The working principle of the preparation device for the hawthorn spider mite control agent with CGG as the carrier provided by the present invention is as follows: Batching process: Prepare a cationic guar gum (CGG) aqueous emulsion by mixing cationic guar gum (CGG) and water in a mass ratio of 1% to 2%, and add it to one of the ingredient barrels 7. At the same time, add an aqueous emulsion containing V-ATPase A dsRNA with a concentration of 0.3% to 0.7% to another ingredient barrel 7. Start the first motor 13. The output end of the first motor 13 is fixedly connected to one end of the ingredient worm 12. Therefore, the rotation of the motor will drive the ingredient worm 12 to rotate. Since the ingredient worm 12 is meshed with the ingredient worm wheel 11, the rotation of the ingredient worm 12 will further drive the ingredient worm wheel 11 and the turntable 10 to rotate together. The rotation of the turntable 10 stirs the solution in the ingredient barrel 7, ensuring the uniform mixing of the raw materials. The design of this ingredient structure not only improves the ingredient efficiency but also ensures the accuracy of the ingredients; Defoaming process: Start the second motor 35. The output end of the second motor 35 is fixedly connected to the transmission pulley 34 away from the first motor 13. Therefore, the rotation of the second motor 35 will drive the transmission pulley 34 to rotate, which in turn drives the rotating rod 33 and the fluid guide 32 to rotate. The rotation of the fluid guide 32 changes the flow direction of the solution, causing it to flow into the defoaming barrel 3 from the bottom of the ingredient barrel 7 through a pipeline. When the solution enters the defoaming barrel 3, start the third motor 17. The output end of the third motor 17 is fixedly connected to the defoaming worm 18. Therefore, the rotation of the third motor 17 will drive the defoaming worm 18 to rotate. The defoaming worm 18 is meshed with the defoaming worm wheel 19. So the rotation of the defoaming worm 18 will drive the defoaming worm wheel 19 to rotate together. The rotation of the defoaming worm wheel 19 drives the second connecting arm 23 and the gear 20 to rotate reciprocally through the transmission of the first connecting arm 21 and the connecting rod 22. At the same time, the driving pulley 24 fixed to the outer wall of the defoaming worm 18 drives the driven pulley 25 to rotate through a belt; A cylindrical rack 26 is slidably connected to the inner wall of the driven pulley 25. The outer wall of the cylindrical rack 26 is provided with an external spline, which is fitted and installed with the internal spline on the inner wall of the driven pulley 25. This design enables the cylindrical rack 26 to rotate while sliding on the inner wall of the driven pulley 25. The rotation of the cylindrical rack 26 drives the defoaming column 27 fixedly connected to the bottom to rotate in the defoaming barrel 3, while the reciprocating rotation of the gear 20 drives the cylindrical rack 26 engaged with it to move reciprocally in the vertical direction, thereby driving the defoaming column 27 fixedly connected to the cylindrical rack 26 to move reciprocally in the vertical direction. The combined action of the rotation and reciprocating movement of the defoaming column 27 efficiently and quickly eliminates the bubbles in the solution. The design of this defoaming structure not only improves the defoaming efficiency but also avoids the influence of bubbles on the effect of the control agent and avoids measurement errors; Mixing process: Start the metering pump 5. The input end of the metering pump 5 is connected to the bottom of the corresponding defoaming barrel 3 through a pipeline. Therefore, the solution in the defoaming barrel 3 can be accurately extracted. The output end of the metering pump 5 is connected to the top of the mixing barrel 4 through a pipeline. So the extracted solution will be transported into the mixing barrel 4. After the solution enters the mixing barrel 4, start the fourth motor 28. The output end of the fourth motor 28 is fixedly connected with a mixing column 29. Therefore, the rotation of the fourth motor 28 will drive the mixing column 29 to rotate. The rotation of the mixing column 29 has a stirring effect on the solution in the mixing barrel 4, ensuring the uniform mixing of different raw materials and finally obtaining a highly stable preventive agent. After the mixing is completed, open the switch valve 30 to take out the solution in the mixing barrel 4; Cleaning process: Start the second motor 35 to drive the transmission pulley 34 to rotate, so that the batching barrel 7 is connected to the waste pipe. Then start the electric push rod 14. The output end of the electric push rod 14 is fixedly connected to the top of the scraping disc 15. Therefore, the telescopic movement of the electric push rod 14 will drive the scraping disc 15 to move up and down, scraping off the solution adhering to the inner wall of the batching barrel 7. This design can not only ensure that there is no residue in the barrel, guarantee the accurate proportioning, but also reduce the pollution and maintenance cost of the equipment.

[0024] The above are only the embodiments of the present invention, and do not limit the patent scope of the present invention accordingly. Any equivalent structure or equivalent process transformation made by using the specification and drawings of the present invention, or directly or indirectly applied in other related technical fields, shall be included in the patent protection scope of the present invention by the same token.

Claims

1. A device for preparing a hawthorn spider mite control agent using CGG as a carrier, characterized in that: include: A base (1), the top of the base (1) is fixedly connected to a partition (2), the top of the base (1) is symmetrically fixedly connected to a defoaming bucket (3) on one side of the partition (2), and the top of the base (1) is fixedly connected to a mixing bucket (4) on the other side of the partition (2); A metering pump (5) is symmetrically fixedly connected to the top of the partition (2), the input end of the metering pump (5) is connected to the bottom of the corresponding defoaming barrel (3) through a pipeline, and the output end of the metering pump (5) is connected to the top of the mixing barrel (4) through a pipeline. A support platform (6), both sides of the base (1) are fixedly connected to the support platform (6), the top of the support platform (6) is fixedly connected to a batching barrel (7), the bottom of the batching barrel (7) is connected to the top of the defoaming barrel (3) and the waste pipe through a pipeline, the top of the support platform (6) is located between the two batching barrels (7) and is fixedly connected to a support plate (8), and the top of the support plate (8) is fixedly connected to a T-shaped support column (9); A batching structure, wherein a batching structure for batching is installed inside the batching barrel (7), the batching structure comprising: a dynamic push rod (14) and a scraper (15), both ends of the T-shaped support column (9) are fixedly connected to the electric push rod (14), the inner wall of the batching barrel (7) is slidably connected to the scraper (15), and the output end of the electric push rod (14) is fixedly connected to the top of the scraper (15); A defoaming structure, wherein a defoaming structure for defoaming is installed inside the support plate (8); A mixing structure for mixing materials is installed inside the mixing barrel (4).

2. The device for preparing the hawthorn spider mite control agent using CGG as a carrier according to claim 1, characterized in that: The batching structure further comprises: a rotating disk (10), a batching worm wheel (11), a batching worm (12), a first motor (13) and a three-way valve (16); the bottoms of the two batching barrels (7) are both rotatably connected to the rotating disk (10); the bottom of the rotating disk (10) is fixedly connected to the batching worm wheel (11); one side of the batching barrel (7) is provided with an opening; the top of the support platform (6) is rotatably connected to the batching worm wheel (12); the batching worm wheel (12) is meshingly connected to the batching worm wheel (11); one side of the support platform (6) is fixedly connected to the first motor (13); the output end of the first motor (13) is fixedly connected to one end of the batching worm wheel (12); and the bottom of the batching barrel (7) is provided with a three-way valve (16) for controlling the flow direction of the solution.

3. The device for preparing the hawthorn spider mite control agent using CGG as a carrier according to claim 1, characterized in that: The defoaming structure comprises: a third motor (17), a defoaming worm (18), a defoaming worm wheel (19), a gear (20), a first connecting arm (21), a connecting rod (22), a second connecting arm (23), a driving pulley (24), a driven pulley (25), a cylindrical rack (26) and a defoaming column (27); the third motor (17) is fixedly connected inside the support plate (8); the output end of the third motor (17) is fixedly connected to the defoaming worm (18); the defoaming worm wheel (19) is symmetrically rotatable inside the support plate (8); the defoaming worm wheel (19) is meshed with the defoaming worm (18); the gear (20) is symmetrically rotatable inside the support plate (8); one side of the defoaming worm wheel (19) is fixedly connected to the defoaming worm (18); A first connecting arm (21) is provided, one end of the first connecting arm (21) is rotatably connected to a connecting rod (22), one side of the gear (20) is fixedly connected to a second connecting arm (23), one end of the connecting rod (22) is rotatably connected to the second connecting arm (23), an outer wall of the defoaming worm (18) is fixedly connected to a driving pulley (24), an inner part of the support plate (8) is symmetrically rotatably connected to a driven pulley (25), the driving pulley (24) is transmission-connected to the driven pulley (25) via a belt, an inner wall of the driven pulley (25) is slidably connected to a cylindrical rack (26), the cylindrical rack (26) is meshingly connected to the gear (20), and a plurality of defoaming columns (27) are equidistantly fixedly connected to the bottom of the cylindrical rack (26).

4. The device for preparing the hawthorn spider mite control agent using CGG as a carrier according to claim 1, characterized in that: The mixing structure comprises: a fourth motor (28), a mixing column (29) and a switch valve (30); the fourth motor (28) is fixedly connected inside the base (1); the output end of the fourth motor (28) is fixedly connected to the mixing column (29); the bottom of the mixing barrel (4) is fixedly connected to a discharge pipe; and the middle of the discharge pipe is provided with a switch valve (30).

5. The device for preparing the hawthorn spider mite control agent using CGG as a carrier according to claim 2, characterized in that: A through hole is provided at the axis of the batching worm wheel (11) and the rotating disk (10), and the diameter of the through hole is the same as the diameter of the pipe connected to the bottom of the batching barrel (7).

6. The device for preparing the hawthorn spider mite control agent using CGG as a carrier according to claim 2, characterized in that: The two three-way valves (16) are each composed of a shell (31), a fluid guide (32) and a rotating rod (33). The bottom of the batching barrel (7) is provided with a shell (31), the shell (31) is fixedly connected to the pipeline, the inner wall of the shell (31) is rotatably connected to the fluid guide (32), one side of the fluid guide (32) is fixedly connected to the rotating rod (33), one end of the rotating rod (33) away from the fluid guide (32) is fixedly connected to a driving pulley (34), the two driving pulleys (34) are connected by belt transmission, the inside of the support platform (6) is fixedly connected to a second motor (35) on a side away from the first motor (13), and the output end of the second motor (35) is fixedly connected to the driving pulley (34) away from the first motor (13).

7. The device for preparing the hawthorn spider mite control agent using CGG as a carrier according to claim 3, characterized in that: The outer wall of the cylindrical rack (26) is provided with an external spline, and the inner wall of the driven pulley (25) is provided with an internal spline which is mounted in cooperation with the external spline.

8. The device for preparing the hawthorn spider mite control agent using CGG as a carrier according to claim 3, characterized in that: The second connecting arm (23) is longer than the first connecting arm (21).

9. The device for preparing the hawthorn spider mite control agent using CGG as a carrier according to claim 3, characterized in that: One side of the multiple groups of defoaming columns (27) is fixedly connected with multiple groups of defoaming needles at equal intervals, and the multiple groups of defoaming columns (27) are installed in a staggered manner.

10. A method for preparing a hawthorn spider mite control agent using CGG as a carrier using the preparation device according to any one of claims 1 to 9, characterized in that: S1: The present invention provides a hawthorn spider mite control agent using cationic guar gum (CGG) as a carrier, wherein the control agent uses cationic guar gum (CGG) as a carrier to encapsulate V-ATPase A dsRNA, and cationic guar gum (CGG) and water are added to one of the batching barrels (7) at a mass ratio of 1% to 2%, and a solution containing V-ATPase A dsRNA and water are added to another batching barrel (7) at a mass ratio of 0.3% to 0.7%; S2: starting the first motor (13), the first motor (13) drives the batching worm (12) to rotate, the batching worm wheel (11) drives the batching worm wheel (11) to rotate, and then drives the rotating disk (10) to rotate to stir the solution in the batching barrel (7); S3: starting the second motor (35), the second motor (35) drives the driving pulley (34) to rotate, thereby driving the rotating rod (33) and the guide body (32) to rotate, so that the batching barrel (7) is connected with the defoaming barrel (3), and the solution is sent into the defoaming barrel (3); S4: starting the third motor (17), the third motor (17) drives the defoaming worm (18) to rotate, thereby driving the driving pulley (24) and the defoaming worm wheel (19) to rotate, the driving pulley (24) drives the driven pulley (25) to rotate via a belt, the driven pulley (25) drives the cylindrical rack (26) to rotate, the cylindrical rack (26) drives the defoaming column (27) to rotate, the defoaming worm wheel (19) drives the first connecting arm (21) to rotate, during the rotation process, the first connecting arm (21) drives the second connecting arm (23) and the gear (20) to reciprocate via the connecting rod (22), the gear (20) drives the cylindrical rack (26) to reciprocate in the vertical direction, and the cylindrical rack (26) drives the defoaming column (27) to reciprocate in the vertical direction; S5: starting the metering pump (5), the metering pump (5) draws the cationic guar gum (CGG) and the dsRNA aqueous emulsion from the batching barrel (7) into the mixing barrel (4), starting the fourth motor (28), the fourth motor (28) drives the mixing column (29) to rotate to stir the solution in the mixing barrel (4); S6: The second motor (35) is started, and the second motor (35) drives the driving pulley (34) to rotate, thereby driving the rotating rod (33) and the guide body (32) to rotate, so that the batching barrel (7) is connected to the waste pipe, and the electric push rod (14) is started, and the electric push rod (14) drives the scraper (15) to move downward and scrape off the solution adhering to the inner wall of the batching barrel (7).

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