Polygonatum sibiricum-containing composition as well as preparation process and application thereof

Abstract

The invention relates to the technical field of traditional Chinese medicine extraction, and provides a composition containing rhizoma polygonati as well as a preparation process and application thereof. The extraction method of the polygonatum sibiricum polysaccharide extract comprises the following steps: S1, mixing polygonatum sibiricum powder with petroleum ether, extracting and separating to obtain a first solid mixture; s2, mixing the first solid mixture with a solvent, and extracting to obtain a first mixed solution; s3, adding ethanol into the first mixed solution, uniformly mixing, standing and separating to obtain a second solid mixture; s4, mixing the second solid mixture with a Sevage reagent, oscillating, separating to obtain a second mixed solution, concentrating, adding ethanol, standing, separating, and drying to obtain a polygonatum polysaccharide extract; the solvent comprises an auxiliary agent and water; and the auxiliary agent comprises 2-hydroxyethyl trimethyl ammonium chloride and hydroxyacetamide. According to the technical scheme, the problems of low extraction rate of the rhizoma polygonati polysaccharide extract and low purity of the rhizoma polygonati polysaccharide in the rhizoma polygonati polysaccharide extract in the prior art are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine extraction, and specifically, to a composition containing polygonatum, its preparation process and application. Background Art

[0002] Polygonatum is a common medicinal plant belonging to the genus Polygonatum of the family Liliaceae. Its rhizome is used as a medicinal material, having various effects such as moistening the lungs and tonifying the kidneys, and can play an important role in treating diseases such as spleen and stomach qi deficiency. It is also one of the key raw materials for many traditional prescriptions and modern Chinese patent medicines. Polygonatum contains various active substances such as polysaccharides, saponins, and flavonoids. Among them, polygonatum polysaccharide is the main active ingredient of polygonatum, which can be extracted from the rhizomes of Liliaceae plants such as Polygonatum kingianum, Polygonatum sibiricum, or Polygonatum cyrtonema, showing multiple pharmacological activities such as anti-aging, anti-tumor, hypoglycemic, antiviral, and regulating the body's immunity. In addition, when polygonatum or its polysaccharide extract is used in combination with other traditional Chinese medicines, more significant pharmacological effects can be exerted.

[0003] In the extraction process of polygonatum polysaccharide, the traditional method mostly uses solvent extraction, such as extracting with hot water, dilute acid or dilute alkali solution. Although this method is simple to operate, however, the extraction time is relatively long, and the extraction rate and purity of polygonatum polysaccharide are relatively low. At the same time, the acid-base environment may also reduce the activity of the polysaccharide, thereby affecting its pharmacological effects.

[0004] In view of this, an extraction method that can improve the extraction rate and purity of polygonatum polysaccharide is proposed, which can better play a compatibility role with other traditional Chinese medicine components and enhance its pharmacological effects. Summary of the Invention

[0005] The present invention provides a composition containing polygonatum, its preparation process and application, which solves the problems of low extraction rate of polygonatum polysaccharide extract and low purity of polygonatum polysaccharide in the related art.

[0006] The technical solution of the present invention is as follows: The present invention provides an extraction method for polygonatum polysaccharide extract, comprising the following steps: S1. Mix polygonatum powder with petroleum ether, extract and separate to obtain a first solid mixture; S2. Mix the first solid mixture with a solvent, extract to obtain a first mixed solution; S3. Add ethanol to the first mixed solution, mix evenly, stand and separate to obtain a second solid mixture; S4. Mix the second solid mixture with Sevage reagent, shake and separate to obtain a second mixed solution. After concentrating the second mixed solution, add ethanol, stand, separate and dry to obtain the polygonatum polysaccharide extract; The solvent includes an auxiliary agent and water; The auxiliary agent includes 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide.

[0007] As a further technical solution, the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:1 to 2.

[0008] When the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:1 to 2, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in the polygonatum polysaccharide extract can be further improved.

[0009] As a further technical solution, the mass ratio of the auxiliary agent to the water is 8:2.4 to 4.

[0010] As a further technical solution, the preparation method of the solvent includes the following steps: after mixing the components of the auxiliary agent, add water and mix evenly to obtain the solvent.

[0011] As a further technical solution, the mixing evenly means stirring until evenly mixed. When stirring, the temperature is 70 to 80 °C, the stirring speed is 300 to 500 r / min, and stir until the solvent is a transparent solvent.

[0012] As a further technical solution, the auxiliary agent further includes methyl glycolate.

[0013] When the auxiliary agent further includes methyl glycolate, through the combined action of 2-hydroxyethyltrimethylammonium chloride, hydroxyacetamide and methyl glycolate, the polygonatum polysaccharide extract can be dissolved more stably and rapidly, thereby further improving the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in the polygonatum polysaccharide extract.

[0014] As a further technical solution, the mass ratio of the sum of the masses of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide to methyl glycolate is 3 to 4:1.

[0015] When the mass ratio of the sum of the masses of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide to methyl glycolate is 3 to 4:1, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in the polygonatum polysaccharide extract can be further improved.

[0016] As a further technical solution, the mass-volume ratio of the polygonatum powder to the petroleum ether is 15 g:100 to 140 mL; The mass-volume ratio of the polygonatum powder to the solvent is 15 g:350 to 550 mL; The mass-volume ratio of the polygonatum powder to the Sevage reagent is 15 g:100 to 130 mL, and the Sevage reagent is composed of chloroform and n-butanol with a volume ratio of 4:1.

[0017] As a further technical solution, in step S1, during the extraction, the temperature is 40 - 50 °C and the time is 2 - 3 h.

[0018] As a further technical solution, in step S3, the addition amount of ethanol is 5 - 6 times the volume of the first mixture.

[0019] As a further technical solution, in step S3, during the standing, the temperature is 5 - 8 °C and the standing time is 8 - 10 h.

[0020] As a further technical solution, in step S4, during the concentration, it is concentrated to 20% - 30% of the volume of the Sevage reagent.

[0021] As a further technical solution, in step S4, the addition amount of ethanol is 1 - 2 times the volume of the Sevage reagent.

[0022] As a further technical solution, in step S4, during the standing, the standing time is 3 - 4 h.

[0023] The present invention also provides a composition containing polygonatum, including the polygonatum polysaccharide extract obtained by the extraction method of the polygonatum polysaccharide extract described above.

[0024] As a further technical solution, it includes the following components in parts by weight: 20 - 40 parts of polygonatum polysaccharide extract, 10 - 16 parts of dried tangerine peel, 8 - 15 parts of poria cocos, 8 - 12 parts of honeysuckle flower, 3 - 8 parts of cassia seed.

[0025] The present invention also provides a preparation process of the composition containing polygonatum, including the following steps: A1. Weigh the components except the polygonatum polysaccharide extract, mix, pulverize, and sieve to obtain a mixed powder; A2. Add the mixed powder into water, decoct, separate to obtain a first filtrate and residues, decoct the residues twice to obtain a second filtrate, combine the first filtrate and the second filtrate, and concentrate to obtain a paste - like mixture; A3. Add the paste - like mixture into water, add the polygonatum polysaccharide extract, and decoct to obtain the composition containing polygonatum.

[0026] As a further technical solution, in step A2, the addition amount of water is 6 - 8 times the mass of the mixed powder, and during the decoction, the temperature is 60 - 70 °C and the time is 2 - 3 h.

[0027] As a further technical solution, in step A3, the addition amount of water is 3 - 4 times the mass of the paste - like mixture, and during the decoction, the temperature is 50 - 60 °C and the time is 1 - 2 h.

[0028] The present invention also provides the use of the composition containing polygonatum or the composition containing polygonatum prepared by the preparation process in promoting digestion of the body.

[0029] The working principle and beneficial effects of the present invention are as follows: In the present invention, the extraction method of polygonatum polysaccharide extract is designed with reasonable steps. By treating polygonatum powder with different solvents, a polygonatum polysaccharide extract with high extraction rate and high purity of polygonatum polysaccharide can be obtained. In step S1, treating the polygonatum powder with petroleum ether can effectively remove fat-soluble impurities, which helps the subsequent better dissolution of the polygonatum polysaccharide extract. In step S2, the solvent is a mixture of an auxiliary agent formed by 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide and water. By using 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide together, it can effectively act on the polygonatum polysaccharide extract, increasing the dissolution degree of the polygonatum polysaccharide extract, thereby significantly improving the extraction rate of the polygonatum polysaccharide extract and the purity of polygonatum polysaccharide in the polygonatum polysaccharide extract. Specific Embodiments

[0030] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without making creative efforts fall within the scope of the present invention.

[0031] Example 1 A method for extracting polygonatum polysaccharide extract, comprising the following steps: S1. Mix 15 g of polygonatum powder with 100 mL of petroleum ether, extract at 40 °C for 3 h, and separate to obtain a first solid mixture; S2. Mix the first solid mixture with 350 mL of solvent, extract to obtain a first mixed solution; S3. Add ethanol (the added amount of ethanol is 5 times the volume of the first mixed solution) to the first mixed solution, mix evenly, stand at 5 °C for 10 h, and separate to obtain a second solid mixture; S4. Mix the second solid mixture with 100 mL of Sevage reagent (Sevage reagent is composed of chloroform and n-butanol with a volume ratio of 4:1), shake, separate to obtain a second mixed solution. After concentrating the second mixed solution to 20% of the volume of the Sevage reagent, add ethanol (the added ethanol has the same volume as the Sevage reagent), stand for 3 h, separate, and dry to obtain the polygonatum polysaccharide extract; The solvent includes an auxiliary agent and water; The auxiliary agent includes 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide; The preparation method of the solvent comprises the following steps: After blending the auxiliary agents composed of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide, add water, and stir at 70 °C and a stirring speed of 300 r / min until the solvent becomes transparent to obtain the solvent; wherein, the mass ratio of the auxiliary agent to water is 8:2.4, and the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:0.5; A preparation process of a composition containing polygonatum sibiricum, comprising the following steps: A1. Weigh 10 parts of dried tangerine peel, 8 parts of poria cocos, 8 parts of honeysuckle flower, and 3 parts of cassia seed, mix, pulverize, and sieve to obtain a mixed powder; A2. Add the mixed powder into water (the added amount of water is 6 times the mass of the mixed powder), decoct at 60 °C for 3 h, separate to obtain a first filtrate and filter residue, decoct the filter residue twice more to obtain a second filtrate, combine the first filtrate and the second filtrate, and concentrate to obtain a paste-like mixture; A3. Add the paste-like mixture into water (the added amount of water is 3 times the mass of the paste-like mixture), then add 20 parts of polygonatum sibiricum polysaccharide extract, and decoct at 50 °C for 2 h to obtain the composition containing polygonatum sibiricum.

[0032] Example 2 An extraction method of polygonatum sibiricum polysaccharide extract, comprising the following steps: S1. Mix 15 g of polygonatum sibiricum powder with 120 mL of petroleum ether, extract at 45 °C for 2.5 h, and separate to obtain a first solid mixture; S2. Mix the first solid mixture with 450 mL of solvent and extract to obtain a first mixed solution; S3. Add ethanol (the added amount of ethanol is 5.5 times the volume of the first mixed solution) into the first mixed solution, mix evenly, let stand at 8 °C for 8 h, and separate to obtain a second solid mixture; S4. Mix the second solid mixture with 115 mL of Sevage reagent (Sevage reagent is composed of chloroform and n-butanol with a volume ratio of 4:1), shake, separate to obtain a second mixed solution, concentrate the second mixed solution to 30% of the volume of the Sevage reagent, then add ethanol (the added amount of ethanol is 2 times the volume of the Sevage reagent), let stand for 4 h, separate, and dry to obtain the polygonatum sibiricum polysaccharide extract; The solvent includes an auxiliary agent and water; The auxiliary agent includes 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide; The preparation method of the solvent comprises the following steps: After blending the auxiliary agents composed of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide, add water, and stir at 75 °C and a stirring speed of 400 r / min until the solvent becomes transparent to obtain the solvent; wherein, the mass ratio of the auxiliary agent to water is 8:3, and the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:0.5; The preparation process of a composition containing polygonatum sibiricum redoute comprises the following steps: A1. Weigh 13 parts of dried tangerine peel, 12 parts of poria cocos, 10 parts of honeysuckle flower, and 5.5 parts of cassia seed, mix them, pulverize them, and sieve them to obtain a mixed powder; A2. Add the mixed powder into water (the added amount of water is 8 times the mass of the mixed powder), decoct at 70 °C for 2 h, separate to obtain a first filtrate and residues, decoct the residues twice again to obtain a second filtrate, combine the first filtrate and the second filtrate, and concentrate to obtain a paste-like mixture; A3. Add the paste-like mixture into water (the added amount of water is 4 times the mass of the paste-like mixture), then add 30 parts of polygonatum sibiricum polysaccharide extract, and decoct at 60 °C for 1 h to obtain the composition containing polygonatum sibiricum redoute.

[0033] Example 3 An extraction method of polygonatum sibiricum polysaccharide extract comprises the following steps: S1. Mix 15 g of polygonatum sibiricum powder with 140 mL of petroleum ether, extract at 50 °C for 2 h, and separate to obtain a first solid mixture; S2. Mix the first solid mixture with 550 mL of solvent and extract to obtain a first mixed solution; S3. Add ethanol (the added amount of ethanol is 6 times the volume of the first mixed solution) into the first mixed solution, mix evenly, stand at 8 °C for 8 h, and separate to obtain a second solid mixture; S4. Mix the second solid mixture with 130 mL of Sevage reagent (Sevage reagent is composed of chloroform and n-butanol with a volume ratio of 4:1), shake, separate to obtain a second mixed solution, concentrate the second mixed solution to 30% of the volume of the Sevage reagent, then add ethanol (the added amount of ethanol is 2 times the volume of the Sevage reagent), stand for 4 h, separate, and dry to obtain the polygonatum sibiricum polysaccharide extract; The solvent comprises an auxiliary agent and water; The auxiliary agent comprises 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide; The preparation method of the solvent comprises the following steps: After blending the auxiliary agents composed of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide, add water, and stir at 80 °C and a stirring speed of 500 r / min until the solvent becomes transparent to obtain the solvent; wherein, the mass ratio of the auxiliary agent to water is 8:4, and the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:0.5; A preparation process of a composition containing polygonatum, comprising the following steps: A1. Weigh 16 parts of dried tangerine peel, 15 parts of poria cocos, 12 parts of honeysuckle flower, and 8 parts of cassia seed, mix, pulverize, and sieve to obtain a mixed powder; A2. Add the mixed powder to water (the added amount of water is 8 times the mass of the mixed powder), decoct at 70 °C for 2 h, separate to obtain a first filtrate and residue, decoct the residue twice more to obtain a second filtrate, combine the first filtrate and the second filtrate, and concentrate to obtain a paste-like mixture; A3. Add the paste-like mixture to water (the added amount of water is 4 times the mass of the paste-like mixture), then add 40 parts of polygonatum polysaccharide extract, and decoct at 60 °C for 1 h to obtain a composition containing polygonatum.

[0034] Example 4 The difference between this example and Example 2 is only that in this example, the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:2.5.

[0035] Example 5 The difference between this example and Example 2 is only that in this example, the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:2.

[0036] Example 6 The difference between this example and Example 2 is only that in this example, the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:1.

[0037] Example 7 The difference between this example and Example 6 is only that in this example, the preparation method of the solvent comprises the following steps: After blending the auxiliary agents composed of 2-hydroxyethyltrimethylammonium chloride, hydroxyacetamide, and methyl glycolate, add water, and stir at 75 °C and a stirring speed of 400 r / min until the solvent becomes transparent to obtain the solvent; wherein, the mass ratio of the auxiliary agent to water is 8:3, the mass ratio of the sum of the mass of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide to the mass of methyl glycolate is 2:1, and the mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:1.

[0038] Example 8 The difference between this example and Example 7 is only that in this example, the mass ratio of the sum of the mass of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide to the mass of methyl glycolate is 5:1.

[0039] Example 9 The difference between this example and Example 7 is only that in this example, the mass ratio of the sum of the mass of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide to the mass of methyl glycolate is 3:1.

[0040] Example 10 The difference between this example and Example 7 is only that in this example, the mass ratio of the sum of the masses of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide to the mass of methyl glycolate is 4:1.

[0041] Comparative Example 1 The difference between this comparative example and Example 1 is only that in this comparative example, the auxiliary agent only includes 2-hydroxyethyltrimethylammonium chloride.

[0042] Comparative Example 2 The difference between this comparative example and Example 1 is only that in this comparative example, the auxiliary agent only includes hydroxyacetamide.

[0043] Comparative Example 3 The difference between this comparative example and Example 1 is only that in this comparative example, 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide are replaced with an equal amount of methyl glycolate.

[0044] The extraction rates of the polygonatum polysaccharide extracts and the purities of the polygonatum polysaccharides in the polygonatum polysaccharide extracts prepared in Examples 1 to 10 and Comparative Examples 1 to 3 are shown in Table 1 below. Among them, the extraction rate (%) = mass of polygonatum polysaccharide extract / mass of polygonatum powder × 100%, and the purity of the polygonatum polysaccharide in the polygonatum polysaccharide extract is determined by the sulfuric acid - anthrone colorimetric method.

[0045] Table 1 Results of the extraction rate of polygonatum polysaccharide extract and the purity of polygonatum polysaccharide

[0046] As can be seen from Table 1, compared with Comparative Examples 1 to 3, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in Example 1 are significantly improved, indicating that in step S2, when the auxiliary agent in the solvent consists of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide, 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide have a synergistic effect, which can significantly improve the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in the polygonatum polysaccharide extract.

[0047] Compared with Examples 2 and 4, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in Examples 5 to 6 are improved, indicating that when the mass ratio of 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide is 3:1 to 2, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in the polygonatum polysaccharide extract can be further improved.

[0048] Compared with Example 6, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in Examples 7 to 10 are improved, indicating that when the auxiliary agent also includes methyl glycolate, through the combined action of 2-hydroxyethyltrimethylammonium chloride, hydroxyacetamide and methyl glycolate, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in the polygonatum polysaccharide extract can be further improved.

[0049] Compared with Examples 7 - 8, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in Examples 9 - 10 are increased, indicating that when the mass sum of 2 - hydroxyethyltrimethylammonium chloride and hydroxyacetamide and the weight ratio to methyl glycolate is 3 - 4:1, the extraction rate of the polygonatum polysaccharide extract and the purity of the polygonatum polysaccharide in the polygonatum polysaccharide extract can be further increased.

[0050] Test Example: Test on Promoting the Digestive Function of the Organism Forty SPF - grade Wistar rats (weighing 120 - 150 g) were taken and divided into a blank control group, Test Group 1, Test Group 2, and Test Group 3, a total of 4 groups, with 10 rats in each group. The test time was 30 days. Blank control group: Each rat was intragastrically administered purified water by gavage for 30 consecutive days, with a dosage of 0.1 mL / 10 g·BW. Test Group 1: Each rat was intragastrically administered the polygonatum - containing composition (prepared in Example 1) by gavage for 30 consecutive days, with a dosage of 0.1 mL / 10 g·BW. Test Group 2: Each rat was intragastrically administered the polygonatum - containing composition (prepared in Example 2) by gavage for 30 consecutive days, with a dosage of 0.1 mL / 10 g·BW. Test Group 3: Each rat was intragastrically administered the polygonatum - containing composition (prepared in Example 3) by gavage for 30 consecutive days, with a dosage of 0.1 mL / 10 g·BW. After 30 days, referring to the determination method of digestive enzymes in the method for testing and evaluating the functions of health foods (2022 edition) of the Ministry of Health, the gastric juice volume, pepsin activity, and pepsin excretion volume of the rats were detected. The detection results are shown in Table 2 below.

[0051]

[0052] As can be seen from Table 2, the gastric juice volume, pepsin activity, and pepsin excretion volume of Test Group 1, Test Group 2, and Test Group 3 are greater than those of the blank control group, and the increase in gastric acid secretion and pepsin activity helps to improve the digestive function of the organism, indicating that the polygonatum - containing compositions prepared in Examples 1 - 3 have the effect of promoting the digestion of the organism.

[0053] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included within the protection scope of the present invention.

Claims

1. A method for extracting polygonatum polysaccharide extract, characterized in that: The following steps are involved: S1, mixing polygonatum powder with petroleum ether, extracting, and separating to obtain a first solid mixture; S2, mixing the first solid mixture with a solvent, extracting, and obtaining a first mixed liquid; S3, adding ethanol to the first mixed solution, mixing evenly, letting stand, and separating to obtain a second solid mixture; S4, mixing the second solid mixture with Sevage reagent, shaking, separating, to obtain a second mixed solution, concentrating the second mixed solution, adding ethanol, standing, separating, and drying to obtain the polygonatum sibiricum polysaccharide extract; The solvent includes an auxiliary agent and water; The auxiliary agents include 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide.

2. The method for extracting a polygonatum polysaccharide extract according to claim 1, characterized in that: The mass ratio of 2-hydroxyethyltrimethylammonium chloride to hydroxyacetamide is 3:1-2; The mass ratio of the auxiliary agent to the water is 8:2.4~4.

3. The method for extracting polygonatum polysaccharide extract according to claim 1, characterized in that: The preparation method of the solvent comprises the following steps: after blending the components of the auxiliary agent, adding water, and mixing evenly to obtain the solvent.

4. The method for extracting a polygonatum polysaccharide extract according to claim 1, characterized in that: The auxiliary agent also includes methyl glycolate.

5. The method for extracting polygonatum polysaccharide extract according to claim 4, characterized in that: The mass ratio of the 2-hydroxyethyltrimethylammonium chloride and hydroxyacetamide to the methyl glycolate is 3-4:

1.

6. The method for extracting polygonatum polysaccharide extract according to claim 1, characterized in that: The mass volume ratio of the polygonatum powder to the petroleum ether is 15g:100-140mL; The mass volume ratio of the polygonatum powder to the solvent is 15g:350~550mL; The mass volume ratio of the polygonatum powder to the Sevage reagent is 15g:100-130mL, and the Sevage reagent is composed of chloroform and n-butanol in a volume ratio of 4:

1.

7. A composition containing Polygonatum sibiricum, characterized in that: The invention relates to a polygonatum polysaccharide extract obtained by extracting the polygonatum polysaccharide extract according to any one of claims 1 to 6.

8. A composition containing polygonatum according to claim 7, characterized in that: The composition comprises the following components in parts by weight: 20-40 parts of polygonatum polysaccharide extract, 10-16 parts of dried tangerine peel, 8-15 parts of tuckahoe, 8-12 parts of honeysuckle, and 3-8 parts of cassia seed.

9. The process for preparing a composition containing polygonatum according to any one of claims 7 to 8, characterized in that: The following steps are involved: A1. Weigh the components except the polygonatum polysaccharide extract, mix, crush, and sieve to obtain a mixed powder; A2, adding the mixed powder into water, decocting, separating, obtaining a first filtrate and a filter residue, decocting the filter residue twice to obtain a second filtrate, combining the first filtrate and the second filtrate, concentrating, and obtaining a paste mixture; A3. Add the paste mixture into water, then add the polygonatum polysaccharide extract, and boil them to obtain the polygonatum-containing composition.

10. Use of the composition containing polygonatum according to any one of claims 7 to 8 or the composition containing polygonatum prepared by the preparation process according to claim 9 in promoting digestion.

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